Detection of uranyl ions by single-hairpin based self-hybridization chain reaction.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-13 DOI:10.1016/j.talanta.2024.127374

Fengyi Lin, Yuxin Cheng, Min Li, Zhi Li, Jianyuan Dai

引用次数: 0

Abstract

Uranium is a toxic radionuclide, and its most stable and common ionic form is water-soluble uranyl ions (UO₂²⁺), which migrates into the environment easily and causes adverse effects on environment and human health. Herein, by cleverly designing the stem of DNA hairpin with palindromic sequence, a self-hybridization chain reaction (SHCR) system was developed for sensitive UO₂²⁺ detection. This detection system showed a good linear correlation between the ratio of fluorescence intensities and UO₂²⁺ concentration within the range of 0.05 nM-20 nM, and the detection limit was calculated to be 0.017 nM. Unlike the traditional HCR system which involves two hairpins, this proposed SHCR system only needs one DNA hairpin, which reduces the complexity of sequence design and experimental operation. And it can be used for the detection of other non-nucleic acid targets by simply changing the target molecule recognition module.

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基于单发夹的自杂交链式反应检测铀酰离子。

铀是一种有毒的放射性核素，其最稳定和最常见的离子形式是水溶性铀酰离子（UO22+），易迁移到环境中，对环境和人体健康造成不利影响。本文通过巧妙地设计带有回文序列的DNA发夹茎，建立了一种灵敏检测UO22+的自杂交链反应（SHCR）体系。该检测系统在0.05 nM-20 nM范围内，荧光强度比与UO22+浓度呈良好的线性相关关系，计算出检测限为0.017 nM。与传统HCR系统需要两个发夹不同，该系统只需要一个DNA发夹，降低了序列设计和实验操作的复杂性。通过简单改变靶分子识别模块，也可用于其他非核酸靶标的检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.