Adaptively spatial PSF removal enables contrast enhancement for multi-layer image fusion in photoacoustic microscopy.

IF 3.1 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2024-12-15 DOI:10.1364/OL.538299

Ting Feng, Hang Li, Haigang Ma

引用次数: 0

Abstract

Optical-resolution photoacoustic microscopy enables cellular-level biological imaging in deep tissues. However, acquiring high-quality spatial images without knowing the point spread function (PSF) at multiple depths or physically improving system performance is challenging. We propose an adaptive multi-layer photoacoustic image fusion (AMPIF) approach based on blind deconvolution and registration. Our findings indicate that the AMPIF method rapidly achieves optimized multi-layer focused fused images with superior resolution and contrast without relying on prior knowledge of the PSF. This method holds significant potential for fast imaging of living biological tissues with enhanced contrast at multiple imaging depths.

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自适应空间PSF去除实现了光声显微镜多层图像融合的对比度增强。

光学分辨率光声显微镜使细胞水平的生物成像在深层组织。然而，在不知道多深度点扩展函数（PSF）或物理改善系统性能的情况下获取高质量的空间图像是具有挑战性的。提出了一种基于盲反卷积和盲配准的自适应多层光声图像融合（AMPIF）方法。我们的研究结果表明，AMPIF方法无需依赖于PSF的先验知识，即可快速获得具有优越分辨率和对比度的优化多层聚焦融合图像。该方法具有在多个成像深度下增强对比度的活体生物组织快速成像的巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.