Product-induced catalytic amplification strategy based on DNA tetrahedron for detection of miRNA-21 in colorectal cancer

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2024-12-12 DOI:10.1016/j.talanta.2024.127354

Kaihang Yu, Zhiyi Wu, Lizhu Yang

引用次数: 0

Abstract

A product-induced catalytic amplification (PICA) strategy had been developed for miRNA-21 detection based on DNA tetrahedron module (DTM). The produced DNA fragment could open hairpin structure and increase the concentration of catalyst, accelerating the circular cleavage reaction on DTM by DNAzyme cleavage. The continuously cleavage of DNAzyme on DTM resulted the greatly enhancement of signal. A favorable linear range was achieved from 20 pM to 5 nM with a limit of detection of 7 pM. Furthermore, through the implementation of the PICA strategy, the overall reaction time experienced a noticeable decrease to 30 min. The assessments of the amplification rate and kinetic constant of the PICA strategy were also conducted. These results highlighted the promising potential of the PICA strategy for practical utilization in serum samples.

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基于DNA四面体的产物诱导催化扩增策略检测结直肠癌miRNA-21。

建立了一种基于DNA四面体模块（DTM）的产物诱导催化扩增（PICA）检测miRNA-21的方法。产生的DNA片段可以打开发夹结构，增加催化剂的浓度，加速DTM被DNAzyme切割的环形切割反应。DNAzyme在DTM上的不断裂解导致信号的显著增强。在20 pM ~ 5 nM范围内具有良好的线性关系，检测限为7 pM。此外，通过实施PICA策略，总体反应时间明显缩短至30 min，并对PICA策略的扩增率和动力学常数进行了评估。这些结果突出了异食异黄酮策略在血清样本中实际应用的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.