{"title":"Bioconversion efficiency and chemical composition of Hermetia illucens larvae fed spent mushroom substrates.","authors":"Anjani Nayak, Martin Rühl, Patrick Klüber","doi":"10.1186/s13568-024-01802-4","DOIUrl":null,"url":null,"abstract":"<p><p>Spent mushroom substrate (SMS) is a by-product remaining after harvesting mushrooms. We evaluated the effect of substituting chicken feed with 0-100% of Pleurotus eryngii and Lentinula edodes SMS at different stocking densities (200-1000 larvae/box) on development, composition, and substrate reduction of black soldier fly (Hermetia illucens) larvae. Although the survival rate was not significantly different, feeding pure SMS led to a low growth rate. The substitution level of SMS negatively correlated with individual larval weight, total harvested biomass, larval growth rate (LGR), feed conversion ratio (FCR), substrate reduction, and waste reduction index (WRI) except for the 20% substitution. Feeding 40% SMS resulted in the highest number of prepupae. In the density experiment, the heaviest larvae (220-239 mg fresh weight) were obtained at 200 larvae/box in the 0% SMS group. The frass residue and FCR decreased with increased density. Remarkably, when feeding 20% SMS at 250 larvae/box, the harvested biomass, LGR, and FCR did not differ significantly from the 0% SMS control, whereas some of the higher densities led to a deterioration. In fact, the frass residue, substrate reduction, and WRI were even improved at 250 larvae/box in the 20% SMS group. The chemical analyses of larvae reared on 20% SMS at 250 larvae/box showed comparable ash and fat contents and a higher protein content compared to the 0% SMS group. Accordingly, up to 20% of a standard diet such as chicken feed can be replaced by low-cost SMS without disadvantages for breeding.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"133"},"PeriodicalIF":3.5000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645357/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-024-01802-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Spent mushroom substrate (SMS) is a by-product remaining after harvesting mushrooms. We evaluated the effect of substituting chicken feed with 0-100% of Pleurotus eryngii and Lentinula edodes SMS at different stocking densities (200-1000 larvae/box) on development, composition, and substrate reduction of black soldier fly (Hermetia illucens) larvae. Although the survival rate was not significantly different, feeding pure SMS led to a low growth rate. The substitution level of SMS negatively correlated with individual larval weight, total harvested biomass, larval growth rate (LGR), feed conversion ratio (FCR), substrate reduction, and waste reduction index (WRI) except for the 20% substitution. Feeding 40% SMS resulted in the highest number of prepupae. In the density experiment, the heaviest larvae (220-239 mg fresh weight) were obtained at 200 larvae/box in the 0% SMS group. The frass residue and FCR decreased with increased density. Remarkably, when feeding 20% SMS at 250 larvae/box, the harvested biomass, LGR, and FCR did not differ significantly from the 0% SMS control, whereas some of the higher densities led to a deterioration. In fact, the frass residue, substrate reduction, and WRI were even improved at 250 larvae/box in the 20% SMS group. The chemical analyses of larvae reared on 20% SMS at 250 larvae/box showed comparable ash and fat contents and a higher protein content compared to the 0% SMS group. Accordingly, up to 20% of a standard diet such as chicken feed can be replaced by low-cost SMS without disadvantages for breeding.
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.