Temporal shifts in the phosphate mobilising bacterial community reveal contrasting succession patterns in response to different phosphorus sources

IF 7.7 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Research Pub Date : 2025-02-01 DOI:10.1016/j.envres.2024.120599

Zengwei Feng , Hong Gao , Ling Ding , Yongqiang Qin , Yang Zhou , Guangda Feng , Qing Yao , Honghui Zhu

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引用次数: 0

Abstract

Background

Phosphate mobilising bacteria (PMB), such as phoD- and pqqC-harbouring bacteria, play a crucial role in mobilising insoluble phosphorus (P) in soil through the secretion of alkaline phosphatase and organic acids.

Objectives

To evaluate the succession pattern of PMB communities in response to different insoluble P sources.

Methods

Five P source treatments, including the addition of KH₂PO₄ (PDP), FePO₄ (FEP), Ca₃(PO₄)₂ (TCP), lecithin (LEC), and a negative control, were established in the soil in a microcosmic system. Subsequently, phoD- and pqqC-harbouring bacterial communities were investigated by sequential sampling and high-throughput sequencing. In addition, PMB strains were isolated from these five treatments, and their phosphate mobilising activity was further analysed.

Results

The effect of the insoluble organic P source (LEC) on the succession of PMB communities consistently exceeded that of insoluble inorganic P (Pi) sources (FEP and TCP). A successively enhanced succession and a successively diminishing succession were observed in PMB communities in FEP and TCP, respectively. Furthermore, the soil AP content significantly increased with incubation time in LEC and FEP. Most of the variation in phoD- and pqqC-harbouring bacterial communities was explained by all P fractions, respectively, while Fe-P and stable organic P fractions determined the PMB communities. Insoluble Pi sources, particularly FEP, tended to enrich more high-performance PMB strains for Fe-P.

Conclusion

This study firstly reveals the dynamic response of PMB communities to different insoluble P sources at the community level in a short time scale and identifies key PMB taxa that respond to different insoluble P sources, particularly Fe-P. It is also the first to provide community evidence and a feasible method for obtaining high-performance PMB strains for Fe-P in subtropical acidic red soils through directed enrichment culture.

Abstract Image

查看原文本刊更多论文

磷酸盐动员细菌群落的时间变化揭示了不同磷源响应的不同演替模式。

背景：磷酸盐动员细菌（PMB）通过分泌碱性磷酸酶和有机酸，在动员土壤中不溶性磷(P)方面发挥着重要作用，如phoD-和pqqc -窝藏细菌。目的：评价不同不溶性磷源对PMB群落演替的影响。方法：在土壤微观系统中建立添加KH2PO4 （PDP）、FePO4 （FEP）、Ca3(PO4)2 （TCP）、卵磷脂（LEC）和阴性对照5个磷源处理。随后，通过序贯采样和高通量测序研究了phoD-和pqqc -的细菌群落。此外，从这5种处理中分离出PMB菌株，并进一步分析其磷酸动员活性。结果：不溶性有机磷源（LEC）对PMB群落演替的影响始终超过不溶性无机磷源（FEP和TCP）。FEP和TCP中PMB群落的演替依次增强，演替依次减弱。在LEC和FEP条件下，随着培养时间的延长，土壤AP含量显著增加。phoD-和pqqc -细菌群落的大部分变化分别由所有P组分解释，而Fe-P和稳定有机P组分决定PMB群落。不溶性磷源，特别是FEP，倾向于培养更高效的Fe-P PMB菌株。结论：本研究首次揭示了短时间尺度下植物群落对不同不溶性磷源的动态响应，确定了植物群落对不同不溶性磷源响应的关键类群，尤其是Fe-P。这也首次为在亚热带酸性红壤中定向富集培养获得Fe-P高性能PMB菌株提供了群落证据和可行方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Research 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

12.60

自引率

8.40%

发文量

2480

审稿时长

4.7 months

期刊介绍： The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.