Temporal shifts in the phosphate mobilising bacterial community reveal contrasting succession patterns in response to different phosphorus sources.

IF 7.7 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Research Pub Date : 2024-12-14 DOI:10.1016/j.envres.2024.120599

Zengwei Feng, Hong Gao, Ling Ding, Yongqiang Qin, Yang Zhou, Guangda Feng, Qing Yao, Honghui Zhu

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引用次数: 0

Abstract

Background: Phosphate mobilising bacteria (PMB), such as phoD- and pqqC-harbouring bacteria, play a crucial role in mobilising insoluble phosphorus (P) in soil through the secretion of alkaline phosphatase and organic acids.

Objectives: To evaluate the succession pattern of PMB communities in response to different insoluble P sources.

Methods: Five P source treatments, including the addition of KH₂PO₄ (PDP), FePO₄ (FEP), Ca₃(PO₄)₂ (TCP), lecithin (LEC), and a negative control, were established in the soil in a microcosmic system. Subsequently, phoD- and pqqC-harbouring bacterial communities were investigated by sequential sampling and high-throughput sequencing. In addition, PMB strains were isolated from these five treatments, and their phosphate mobilising activity was further analysed.

Results: The effect of the insoluble organic P source (LEC) on the succession of PMB communities consistently exceeded that of insoluble inorganic P (Pi) sources (FEP and TCP). A successively enhanced succession and a successively diminishing succession were observed in PMB communities in FEP and TCP, respectively. Furthermore, the soil AP content significantly increased with incubation time in LEC and FEP. Most of the variation in phoD- and pqqC-harbouring bacterial communities was explained by all P fractions, respectively, while Fe-P and stable organic P fractions determined the PMB communities. Insoluble Pi sources, particularly FEP, tended to enrich more high-performance PMB strains for Fe-P.

Conclusion: This study firstly reveals the dynamic response of PMB communities to different insoluble P sources at the community level in a short time scale and identifies key PMB taxa that respond to different insoluble P sources, particularly Fe-P. It is also the first to provide community evidence and a feasible method for obtaining high-performance PMB strains for Fe-P in subtropical acidic red soils through directed enrichment culture.

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来源期刊

Environmental Research 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

12.60

自引率

8.40%

发文量

2480

审稿时长

4.7 months

期刊介绍： The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.