CsIREH1 phosphorylation regulates DELLA protein affecting plant height in cucumber (Cucumis sativus)

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-12-13 DOI:10.1111/nph.20309

Hongjiao Zhao, Piaoyun Sun, Can Tong, Xiangbao Li, Tongwen Yang, Yanxin Jiang, Bosi Zhao, Junyang Dong, Biao Jiang, Junjun Shen, Zheng Li

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引用次数: 0

Abstract

Plant height is a critical agronomic trait that affects crop yield, plant architecture, and environmental adaptability. Gibberellins (GAs) regulate plant height, with DELLA proteins acting as key repressors in the GA signaling pathway by inhibiting GA-induced growth. While DELLA phosphorylation is essential for regulating plant height, the precise mechanisms underlying this process remain incompletely understood.
In this study, we identified a cucumber mutant with delayed growth, which exhibited reduced sensitivity to GA treatment. Through bulked segregant analysis (BSA-seq) combined with molecular marker linkage analysis, we successfully identified and cloned the gene responsible for the dwarf phenotype, CsIREH1 (INCOMPLETE ROOT HAIR ELONGATION 1), which encodes an AGC protein kinase.
Further research revealed that CsIREH1 interacts with and phosphorylates DELLA proteins, specifically targeting CsGAIP and CsGAI2. We propose that IREH1-dependent phosphorylation of DELLA proteins prevents their excessive accumulation, thereby maintaining normal plant growth.
Therefore, investigating the role of IREH1-mediated DELLA phosphorylation provides valuable insights and theoretical foundations for understanding how plants regulate growth mechanisms.

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CsIREH1磷酸化调控影响黄瓜株高的DELLA蛋白

株高是影响作物产量、植株结构和环境适应性的重要农艺性状。赤霉素（giberellins, GAs）调节植株高度，其中DELLA蛋白作为GA信号通路的关键抑制因子，通过抑制GA诱导的生长。虽然DELLA磷酸化对调节植物高度至关重要，但这一过程的确切机制尚不完全清楚。在这项研究中，我们鉴定了一个生长延迟的黄瓜突变体，它对GA处理的敏感性降低。通过大量分离分析（BSA-seq）结合分子标记连锁分析，我们成功地鉴定并克隆了导致矮化表型的基因CsIREH1 (INCOMPLETE ROOT HAIR伸长1)，该基因编码AGC蛋白激酶。进一步研究发现，CsIREH1与DELLA蛋白相互作用并磷酸化，特异性靶向CsGAIP和CsGAI2。我们认为ireh1依赖的DELLA蛋白磷酸化阻止了它们的过度积累，从而维持了植物的正常生长。因此，研究ireh1介导的DELLA磷酸化的作用为了解植物如何调节生长机制提供了有价值的见解和理论基础。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.