An alternate route for cellulose microfibril biosynthesis in plants

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2024-12-13 DOI:10.1126/sciadv.adr5188

Eric M. Roberts, Kai Yuan, Arielle M. Chaves, Ethan T. Pierce, Rosalie Cresswell, Ray Dupree, Xiaolan Yu, Richard L. Blanton, Shu-Zon Wu, Magdalena Bezanilla, Paul Dupree, Candace H. Haigler, Alison W. Roberts

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引用次数: 0

Abstract

Similar to cellulose synthases (CESAs), cellulose synthase–like D (CSLD) proteins synthesize β-1,4-glucan in plants. CSLDs are important for tip growth and cytokinesis, but it was unknown whether they form membrane complexes in vivo or produce microfibrillar cellulose. We produced viable CESA-deficient mutants of the moss Physcomitrium patens to investigate CSLD function without interfering CESA activity. Microscopy and spectroscopy showed that CESA-deficient mutants synthesize cellulose microfibrils that are indistinguishable from those in vascular plants. Correspondingly, freeze-fracture electron microscopy revealed rosette-shaped particle assemblies in the plasma membrane that are indistinguishable from CESA-containing rosette cellulose synthesis complexes (CSCs). Our data show that proteins other than CESAs, most likely CSLDs, produce cellulose microfibrils in P. patens protonemal filaments. The data suggest that the specialized roles of CSLDs in cytokinesis and tip growth are based on differential expression and different interactions with microtubules and possibly Ca 2+ , rather than structural differences in the microfibrils they produce.

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与纤维素合成酶（CESAs）类似，纤维素合成酶样 D（CSLD）蛋白也能在植物体内合成 β-1,4-葡聚糖。CSLD 对顶端生长和细胞分裂很重要，但它们在体内是否形成膜复合物或产生微纤维素尚不清楚。我们在不干扰 CESA 活性的情况下，培育出了藓类 Physcomitrium patens 的 CESA 缺失突变体，以研究 CSLD 的功能。显微镜和光谱分析显示，CESA缺陷突变体合成的纤维素微纤维与维管植物中的纤维素微纤维无异。相应地，冷冻断裂电子显微镜显示质膜上的莲座状颗粒集合体与含有 CESA 的莲座状纤维素合成复合物（CSCs）无异。我们的数据表明，除 CESA 外，还有其他蛋白质（最有可能是 CSLD）在 P. patens 原丝中产生纤维素微纤维。这些数据表明，CSLDs 在细胞分裂和顶端生长过程中的特殊作用是基于不同的表达以及与微管和 Ca 2+ 的不同相互作用，而不是它们产生的微纤维在结构上的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.