Effects of Gamma Irradiation on Structural, Chemical, Bioactivity and Biocompatibility Characteristics of Bioactive Glass–Polymer Composite Film

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part A Pub Date : 2024-12-13 DOI:10.1002/jbm.a.37842

Mamun Khan Sujon, Siti Fatimah Samsurrijal, Ruzalina Baharin, Naurah Mat Isa, Muhammad Azrul Zabidi, Siti Noor Fazliah Mohd Noor

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引用次数: 0

Abstract

Gamma irradiation is an effective technique for biocomposite films intended for application in tissue engineering (TE) to ensure sterility and patient safety prior to clinical applications. This study proposed a biocomposite film composed of natural polymer chitosan (CS) and synthetic polymer poly-Ɛ-caprolactone (PCL) reinforced with sol–gel-derived bioactive glass (BG) for potential application in TE. The BG/PCL/CS biocomposite film was sterilized using 25 kGy gamma rays, and subsequent changes in its characteristics were analyzed through mechanical and physical assessment, bioactivity evaluation via immersion in simulated body fluid (SBF) and biocompatibility examination using human primary dermal fibroblasts (HPDFs). Results indicated a homogeneous distribution of BG particles within the BG/PCL/CS polymer matrix which enhanced bioactivity, and the polymer blend provide a structurally stable film. Gamma irradiation induced an increase in the film's surface roughness due to photo-oxidative degradation; however, this did not adversely affect the integrity of glass particles and polymer chains. In vitro assessments demonstrated hydroxyapatite formation on the film's surface, suggesting bioactivity. Biocompatibility testing confirmed enhanced cell adhesion and proliferation. These multifunctional properties highlight the potential of the fabricated BG/PCL/CS biocomposite film for TE and regenerative medicine applications.

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伽马辐照是应用于组织工程（TE）的生物复合膜的有效技术，可确保临床应用前的无菌性和患者安全。本研究提出了一种由天然聚合物壳聚糖（CS）和合成聚合物聚己内酯（PCL）以及溶胶凝胶衍生生物活性玻璃（BG）增强的生物复合膜，有望应用于组织工程（TE）。使用 25 kGy 伽马射线对 BG/PCL/CS 生物复合薄膜进行灭菌，并通过机械和物理评估、浸泡在模拟体液 (SBF) 中进行生物活性评估以及使用人类原生真皮成纤维细胞 (HPDF) 进行生物相容性检查，分析其随后的特性变化。结果表明，BG 颗粒在 BG/PCL/CS 聚合物基质中分布均匀，增强了生物活性，而且聚合物混合物提供了结构稳定的薄膜。由于光氧化降解，伽马射线照射导致薄膜表面粗糙度增加；但这并没有对玻璃微粒和聚合物链的完整性产生不利影响。体外评估显示，薄膜表面形成了羟基磷灰石，这表明薄膜具有生物活性。生物相容性测试证实，细胞粘附和增殖能力增强。这些多功能特性凸显了所制造的 BG/PCL/CS 生物复合薄膜在 TE 和再生医学应用方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part A 工程技术-材料科学：生物材料

CiteScore

10.40

自引率

2.00%

发文量

135

审稿时长

3.6 months

期刊介绍： The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.