Photodynamic antibacterial evaluation of polydopamine nanoparticle optimised Curcumin Longa against endodontic biofilm—An in-vitro study

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2024-12-13 DOI:10.1111/aej.12910

Gokul Gunasekaran, Manavalan Madhana Madhubala MDS, G. S. Nayanthara, Sekar Mahalaxmi

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Abstract

This study aims to evaluate the effect of antimicrobial photodynamic therapy(aPDT) with polydopamine nanoparticle functionalised with Curcuma longa(nPD-Cur) against root canal biofilm. nPD-Cur was prepared and characterised using Scanning Electron Microscopy(SEM), dynamic light scattering(DLS), Fourier-transform infrared spectroscopy(FTIR) and Ultraviolet visual(UV/Vis) spectrophotometry. Root sections (10 mm length) were obtained from 53 single-rooted human premolars and chemo-mechanically prepared followed by inoculation with E. faecalis. All the specimens were randomly divided into five groups(n = 10) and irrigated (Group 1—Saline; Group 2—2.5% Sodium hypochlorite(NaOCl); Group 3—Cur; Group 4—nPD; and Group 5—nPD-Cur) followed by diode irradiation and analysed for reduction in colony-forming units(CFU)/mL, bacterial viability using Confocal Laser Scanning Microscopy(CLSM) and SEM for biofilm disruption. Results were analysed using one-way ANOVA followed by post hoc Tukey's test for pairwise comparison (p < 0.05). nPD-Cur revealed the characteristic absorption patterns. The antimicrobial potency was highest for NaOCl followed by nPD > Cur-nPD > Cur.

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聚多巴胺纳米颗粒优化姜黄素龙骨对牙髓生物膜的光动力抗菌效果研究。

本研究旨在评价姜黄功能化聚多巴胺纳米颗粒（nPD-Cur）抗菌光动力治疗（aPDT）对根管生物膜的作用。采用扫描电子显微镜（SEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和紫外分光光度法（UV/Vis）对nPD-Cur进行了表征。从53个单根人前磨牙中获得10 mm长的根切片，接种粪肠杆菌后进行化学机械制备。所有标本随机分为5组（n = 10），分别灌胃(1组：生理盐水；2-2.5%次氯酸钠（NaOCl）组；组3-Cur;组4-nPD;和5-nPD-Cur组)，然后进行二极管照射，并使用共聚焦激光扫描显微镜（CLSM）和扫描电镜（SEM）分析生物膜破坏情况，分析菌落形成单位(CFU)/mL的减少情况。结果采用单因素方差分析，然后采用事后Tukey检验进行两两比较（p Cur- npd > Cur）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.