The immune defense response and immune-related genes expression in Odontobutis potamophila infected by Aeromonas salmonicida.

Xinhai Zhu, Qi Dong, Xiaoyu Cai, Jia Yin, Yan Liu, Xiaojian Gao, Qun Jiang, Guoxing Liu, Xiaojun Zhang
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Abstract

Aeromonas salmonicida belongs to the Aeromonas family, which could widely infect economic fish, causing diseases and huge economic losses. Recently, A. salmonicida was also detected in diseased Odontobutis potamophila. Transcriptomic model of A. salmonicida-infected O. potamophila was analyzed to reveal immune response. A total of 113,282 unigenes were obtained and annotated in six databases. After 12 h of infection with A. salmonicides, a total of 614 differentially expressed genes (DEGs) (355 up-regulated genes and 259 down-regulated genes) were identified in the head kidney tissues. Following 24 h of infection, a total of 1689 DEGs were detected in the head kidney tissues, including 313 up-regulated genes and 1376 down-regulated genes. GO and KEGG pathway analyses were conducted to provide functional insights and a clearer understanding of the signal transduction pathways associated with the DEGs. Further analysis of the complement and coagulation cascades pathway and PPAR signaling pathway exhibited that the expression of immune genes was widely activated at the beginning of A. salmonicides infection. Additionally, six DEGs were randomly selected and validated using quantitative real-time PCR, showing expression patterns consistent with the high-throughput sequencing data. These results offer important insights that enhance the understanding of immune response in O. potamophila against A. salmonicida infection.

沙门氏气单胞菌感染嗜马铃薯齿虫的免疫防御反应及免疫相关基因表达。
沙门氏气单胞菌属于气单胞菌科,可广泛感染经济鱼类,造成疾病和巨大的经济损失。最近,在患病的嗜黄齿蟾中也发现了沙门氏菌。本研究分析了沙门氏菌感染嗜马铃薯弧菌的转录组学模型,以揭示免疫应答。在6个数据库中共获得并标注了113282个unigenes。感染沙门氏菌12 h后,在头肾组织中共鉴定出614个差异表达基因(deg),其中上调基因355个,下调基因259个。感染24 h后,在头肾组织中共检测到1689个deg,其中上调基因313个,下调基因1376个。进行GO和KEGG通路分析,以提供功能见解,并更清楚地了解与deg相关的信号转导通路。进一步对补体、凝血级联通路和PPAR信号通路的分析表明,在沙门氏菌感染初期,免疫基因的表达被广泛激活。此外,随机选择6个deg并使用定量实时PCR进行验证,显示出与高通量测序数据一致的表达模式。这些结果提供了重要的见解,增强了对嗜马铃薯菌对沙门氏菌感染的免疫反应的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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