Development of a toolbox to study Phytophthora cryptogea - lettuce interactions: Characterization and detection of P. cryptogea in hydroponic lettuce.

IF 4.4 2区 农林科学 Q1 PLANT SCIENCES
Liese Vlasselaer, Joram Moons, Tim De Clercq, Bart Lievens, Barbara De Coninck
{"title":"Development of a toolbox to study <i>Phytophthora cryptogea</i> - lettuce interactions: Characterization and detection of <i>P. cryptogea</i> in hydroponic lettuce.","authors":"Liese Vlasselaer, Joram Moons, Tim De Clercq, Bart Lievens, Barbara De Coninck","doi":"10.1094/PDIS-09-24-2018-RE","DOIUrl":null,"url":null,"abstract":"<p><p>Climate change poses a major threat to crop production, resulting in the emergence of new pests and diseases. <i>Phytophthora cryptogea</i> has recently emerged as a major concern in hydroponic lettuce cultivation, causing substantial yield and economic losses. This oomycete pathogen thrives in elevated water temperatures induced by warmer weather conditions (e.g., heatwaves), facilitating rapid pathogen propagation. Although the disease is already present for several decades in chicory cultivation, where it originates from the field, its origin in lettuce cultivation remains unclear. To get a better understanding of its origin, we conducted a multilocus sequence analysis (MLSA) using five reference genes (ITS, β-tub, COI, EF1α and HSP90) and 33 <i>P. cryptogea</i> isolates from various hosts, including chicory and lettuce. Results revealed a clear separation between lettuce and chicory isolates. Furthermore, we developed and implemented a robust disease bioassay and qPCR assay to investigate the interaction between <i>P. cryptogea</i> strains and lettuce. Our findings revealed that while lettuce isolates exhibited the highest virulence, some chicory isolates also caused disease in lettuce, suggesting a potential evolutionary link between <i>P. cryptogea</i> in lettuce and chicory. Our experiments also revealed that even a low concentration of zoospores (100 zoospores/L) can elicit severe symptoms, underscoring the pathogen's high virulence. Therefore, effective disease management strategies are needed for controlling (the spread of) the disease. Together, this research provides several tools that can be used to enhance our understanding of the interaction between <i>P. cryptogea</i> and its host plants, including the development of proper disease management strategies.</p>","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PDIS-09-24-2018-RE","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Climate change poses a major threat to crop production, resulting in the emergence of new pests and diseases. Phytophthora cryptogea has recently emerged as a major concern in hydroponic lettuce cultivation, causing substantial yield and economic losses. This oomycete pathogen thrives in elevated water temperatures induced by warmer weather conditions (e.g., heatwaves), facilitating rapid pathogen propagation. Although the disease is already present for several decades in chicory cultivation, where it originates from the field, its origin in lettuce cultivation remains unclear. To get a better understanding of its origin, we conducted a multilocus sequence analysis (MLSA) using five reference genes (ITS, β-tub, COI, EF1α and HSP90) and 33 P. cryptogea isolates from various hosts, including chicory and lettuce. Results revealed a clear separation between lettuce and chicory isolates. Furthermore, we developed and implemented a robust disease bioassay and qPCR assay to investigate the interaction between P. cryptogea strains and lettuce. Our findings revealed that while lettuce isolates exhibited the highest virulence, some chicory isolates also caused disease in lettuce, suggesting a potential evolutionary link between P. cryptogea in lettuce and chicory. Our experiments also revealed that even a low concentration of zoospores (100 zoospores/L) can elicit severe symptoms, underscoring the pathogen's high virulence. Therefore, effective disease management strategies are needed for controlling (the spread of) the disease. Together, this research provides several tools that can be used to enhance our understanding of the interaction between P. cryptogea and its host plants, including the development of proper disease management strategies.

气候变化对作物生产构成重大威胁,导致新病虫害的出现。隐杆线虫最近已成为水培生菜种植中的一个主要问题,造成了巨大的产量和经济损失。这种卵菌病原体在天气变暖(如热浪)引起的水温升高时茁壮成长,有利于病原体的快速繁殖。虽然这种病菌在菊苣种植中已经存在了几十年,而且是从田间传入的,但它在莴苣种植中的起源仍不清楚。为了更好地了解其起源,我们使用五个参考基因(ITS、β-tub、COI、EF1α 和 HSP90)和 33 个来自不同寄主(包括菊苣和莴苣)的隐球菌分离物进行了多焦点序列分析(MLSA)。结果显示,莴苣和菊苣分离物之间存在明显的差异。此外,我们还开发并实施了一种可靠的疾病生物测定和 qPCR 分析方法,以研究隐翅虫菌株与莴苣之间的相互作用。我们的研究结果表明,虽然莴苣分离株的毒力最强,但一些菊苣分离株也会导致莴苣发病,这表明隐翅虫在莴苣和菊苣中存在潜在的进化联系。我们的实验还发现,即使是低浓度的子孢子(100 个子孢子/升)也能引起严重的症状,这凸显了病原体的高致病性。因此,需要有效的疾病管理策略来控制该疾病的(传播)。总之,这项研究提供了几种工具,可用于加深我们对隐翅虫与其寄主植物之间相互作用的了解,包括制定适当的病害管理策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant disease
Plant disease 农林科学-植物科学
CiteScore
5.10
自引率
13.30%
发文量
1993
审稿时长
2 months
期刊介绍: Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信