Modified Cajal's trichrome stain in oral squamous cell carcinoma.

Q3 Medicine
S S Vidya Srinidhi, Ambika Murugesan, Sekar Balakrishnan, Maya Ramesh, V Varrshana, Mathew Jacob
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引用次数: 0

Abstract

Background: Modified Cajal's trichrome stain (MCTS) is a good differential stain that allows one to visibly distinguish between connective tissue and epithelial elements with different tonalities of colour.

Aim: Our study aims to evaluate and analyse the effectiveness of oral squamous cell carcinoma (OSCC) using MCTS.

Materials and methods: A study was conducted retrospectively with 30 tissue blocks embedded in paraffin from cases of OSCC that have been confirmed by histopathology. Both standard haematoxylin and eosin (H&E) and MCTS were applied to each section. Then all the sections were analysed by two observers for nucleus cytoplasmic intensity, break in the basement membrane, and advancing front of the tumour, muscle, and surrounding stroma. The efficacy of the stain was assessed and was graded as 1, poor; 2, fair; and 3, good based on the staining intensity.

Statistical analysis: The parameters were graded for H&E and modified Cajal's stain. The results were subjected to the Chi-square test.

Result: The above-mentioned parameters analysed showed a uniformly significant P value of 0.001 for comparing modified Cajal's trichrome stain to H&E stain. Measurement of the agreement was done based on Kappa statistics between two observers, and the values for each expression show that there was good agreement between the two for all the parameters.

Conclusion: MCTS can also be used as a diagnostic aid to pathologists for better distinction of cellular components and easier identification, thereby solving difficulties in diagnosis at earlier stages.

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来源期刊
Journal of Oral and Maxillofacial Pathology
Journal of Oral and Maxillofacial Pathology Medicine-Otorhinolaryngology
CiteScore
1.40
自引率
0.00%
发文量
115
期刊介绍: The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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