{"title":"Poster Presentations","authors":"","doi":"10.1111/jgh.16780","DOIUrl":null,"url":null,"abstract":"<p><b>PP-01-001</b></p><p><b>Long-term prognosis after ESD for superficial spreading esophageal squamous cell carcinoma of ≥50 mm</b></p><p><b>Satoshi Masuda</b><sup>1</sup>, Yuji Urabe<sup>1</sup>, Takahiro Uda<sup>1</sup>, Tomoyuki Gurita<sup>1</sup>, Yoshiki Hatsushika<sup>1</sup>, Yukiko Sako<sup>1</sup>, Takeo Nakamura<sup>1</sup>, Kazuki Ishibashi<sup>1</sup>, Hirona Konishi<sup>1</sup>, Junichi Mizuno<sup>1</sup>, Yuichi Hiyama<sup>1</sup>, Akira Ishikawa<sup>2</sup>, Toshio Kuwai<sup>1</sup> and Shiro Oka<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Hiroshima University Hospital;</i> <sup>2</sup><i>Department of Molecular Pathology, Graduate School of Biomedical and Health Sciences, Hiroshima University</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To evaluate the outcomes and prognosis after ESD for superficial spreading esophageal squamous cell carcinoma (SESCC).</p><p><b><i>Subjects and Methods:</i></b> We examined treatment outcomes (en bloc resection rate, tumor depth, lymphovascular invasion, and complications) in 79 patients with SESCC of 50 mm or more diameter and 79 lesions who underwent ESD between 2011 and 2018 at our department (observation period 86.9±33.2 months).</p><p><b><i>Results:</i></b> En bloc resection rate was 99% (78/79). Tumor depth was pT1a-EP/LPM 45 (57%), pT1a-MM 16 (20%), pT1b-SM1 5 (6%), pT1b-SM2 13 (16%), lymphovascular invasion was observed in 12 (15%). No postoperative bleeding, intraoperative perforation in 4 (5%), and refractory stenosis (≥5 times endoscopic balloon dilatation) in 32 (41%) were observed. No local recurrence was observed, and 16 patients received additional treatment (CRT in 12, and operation in 4). Prognosis was 91%/84% for overall survival, 87%/80% for recurrence-free survival, and 77%/67% for disease-free surviva (3/5 years). Lymph node or other organ metastasis was observed in 6 patients (pT1a-MM,Ly0,V0/pT1b-SM1/pT1b-SM2 3/1/2). Death due to the primary disease was observed in 4 patients (pT1a-MM,Ly0,V0/pT1b-SM1/pT1b-SM2 2/1/1).</p><p><b><i>Conclusion:</i></b> Although ESD for SESCC could be safely performed with en bloc resection, metastasis and primary death were observed in pT1a-MM lymphovascular invasion-negative cases that did not receive any additional treatment.</p><p><b>PP-01-002</b></p><p><b>A rare case of mycobacterium avium complex enteritis in advanced HIV</b></p><p><b>Isaac Chan</b><sup>1</sup>, Alyssa Sim<sup>2</sup>, Raymond Liang<sup>2</sup>, Hwei Yee Lee<sup>2</sup> and Stephanie Sutjipto<sup>3</sup></p><p><sup>1</sup><i>National Healthcare Group, Singapore, Singapore;</i> <sup>2</sup><i>Tan Tock Seng Hospital, Singapore, Singapore;</i> <sup>3</sup><i>National Centre for Infectious Diseases, Singapore, Singapore</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Mycobacterium Avium Complex (MAC) infection is an AIDS-defining opportunistic infection seen in advanced human immunodeficiency virus (HIV). However, it rarely involves the gastrointestinal (GI) tract and endoscopic findings may be non-specific. We report a case of disseminated MAC infection with GI involvement in a patient with advanced HIV.</p><p><b><i>Case Description:</i></b> A 43-year-old male with untreated HIV since diagnosis in 2016 presented with chronic, refractory diarrhoea and significant weight loss. His CD4 count was< 20 cells/uL. Evaluation of diarrhoea was negative for C.difficile toxin, stool cultures, microscopy and parasites. Computed tomography (CT) revealed hepatomegaly, diffuse lymphadenopathy and small-bowel thickening. Oesophagogastroduodenoscopy (OGD) revealed non-specific gastric erosions but showed diffuse, scattered white nodules involving the entire second part of duodenum with atrophic mucosa. However, normal mucosa was seen on sigmoidoscopy. Biopsies from abnormal duodenal mucosa, random gastric and rectal biopsies revealed diffuse foamy macrophages in the lamina propria, duodenal villous blunting and the presence of acid-fast bacillus organisms, without evidence of cytomegalovirus. This was also seen on lymph node biopsies. A diagnosis of disseminated MAC with GI involvement was made and treatment with rifabutin, azithromycin and ethambutol was initiated.</p><p><b><i>Discussion:</i></b> 20-40% of patients with CD4 count < 50 will develop disseminated MAC infection. Rarely, there will be GI involvement with manifestations of diarrhea, abdominal pain or non-specific systemic symptoms. Endoscopic findings may not be typical. Vigilance and early recognition to obtain tissue for histology is crucial for diagnosis in suspected patients. Currently, the role of repeat endoscopy for treatment follow-up is unclear.</p><p><b>PP-01-003</b></p><p><b>An unusual case of rectal pain: migration of a decade-old IUCD, successfully managed endoscopically</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Githma Wimalasena<sup>1</sup>, Duminda Subasinghe<sup>1,2</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital Sri Lanka, Colombo, Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Intrauterine contraceptive device (IUCD) is an effective and safe contraceptive method with few known complications which includes uterine perforation and migration. Here we present a unique case of an IUCD migrating across the vagina to the rectum and causing rectal pain and per-vaginal gas discharge, 10 years after insertion.</p><p><b><i>Case Description:</i></b> A 38-year-old female presented with rectal pain and passing gas through vagina for 4 months. An IUCD was inserted 10 years back. Digital rectal examination revealed protruding rod like object anteriorly. Sigmoidoscopy showed a plastic tubular stump protruding through the anterior rectal wall. The loop was removed through the scope using a snare and the 3mm defect which appeared epithelialized was coagulated and closed with 3 endo-clips (Resolution 360TM Clip). The passage of gas from the vagina and rectal pain subsided and she remains asymptomatic 6 months post-intervention.</p><p><b><i>Discussion:</i></b> Migration is a rare but recognized complication of IUCDs and should be suspected when the threads are not palpable on examination. Migration can occur to the bladder, omentum, intestines and rectum. They may cause significant complications such as peritonitis, bowel perforation, fistula and abscess formation. Retrieval can be done via hysteroscopy, endoscopy or laparoscopically depending on the location and complications. Endoscopic closure is successful in accessible sites like the intestines. Regular self-examination and follow-up are recommended for early detection. Endoscopic retrieval and closure of defects is a successful non-invasive method when the IUCD limbs are visible through the intestines.</p><p><b>PP-01-004</b></p><p><b>Beaver tail liver –Laparoscopic and computed tomographic views of a rare hepatic morphology</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Prabhath Madhuwantha<sup>3</sup>, Uditha Kodithuwakku<sup>4</sup>, Sivasooriya Siwaganesh<sup>2,3</sup> and Duminda Subasinghe<sup>2,3</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, National Hospital Sri Lanka, Colombo;</i> <sup>4</sup><i>Department of Radiology, National Hospital of Sri Lanka, Colombo</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Beaver tail of the liver(BTL) is a rare morphological lobar anatomical variant. It is characterized by extension of liver segment II/III beyond anterior margin of spleen. It is difficult to differentiate from spleen in imaging as echogenicity or density is similar and can lead to unnecessary investigations. This can mimic other pathologies like splenic subcapsular haematoma, peri-splenic fluid collections.</p><p><b><i>Case Description:</i></b> A 62-year-old, ASA II male presented with anorexia, evening pyrexia and weight loss for one year. His serum biochemistry was normal except for an elevated erythrocyte sedimentation rate. Ultrasound abdomen showed splenomegaly and para-aortic lymph nodes suggestive of lymphoma. His CT abdomen incidentally detected BTL with the left lobe of liver encircling the spleen. He underwent a diagnostic laparoscopy for tissue diagnosis and revealed an additional extension liver parenchyma from left lateral segment of the liver encircling the spleen resembling the tail of a beaver. He had laparoscopic liver wedge biopsy for confirmation of the diagnosis.</p><p><b><i>Discussion:</i></b> There can be ectopic, accessory lobes of the liver. One of the most commonly found accessory lobes corresponds to hypertrophied segment V/VI, best known as Riedel’s lobe. It is important to emphasize how the unexpected presence of the left hepatic lobe in the upper left quadrant of the abdomen can lead to misinterpretations in cross-sectional imaging. This case highlights the rare and unique challenges BTL presents to surgeons and radiologists and the importance of knowledge of rare hepatic anatomical variations in interpreting imaging studies.</p><p><b>PP-01-005</b></p><p><b>Hepatic tuberculosis mimicking hepatic metastases of a lymphoma –A rare case diagnosed with laparoscopy</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Githma Wimalasena<sup>1</sup>, Sivasooriya Siwaganesh<sup>2,3</sup> and Duminda Subasinghe<sup>2,3</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, National Hospital Sri Lanka, Colombo</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hepatic tuberculosis(TB) without active pulmonary TB is quite rare. An estimate of the incidence is around 1% of all active TB cases. It manifests as thickened liver capsule with subcapsular nodular lesions and is referred to as “sugar-coated” or “frosted” liver.</p><p><b><i>Case Description:</i></b> A 62-year-old, ASA II male presented with anorexia, evening pyrexia, and loss of weight for one year, with USS findings of splenomegaly and para-aortic lymph nodes and mediastinal lymphadenopathy on HRCT. His basic serum biochemistry was normal except for an elevated ESR of 100. His LDH was 105U/L (125-220). A CT abdomen was performed revealing multiple small non-enhancing hypoattenuating ill-defined lesions noted in both lobes of the liver, suggestive of lymphoma with involvement of the liver. A diagnostic laparoscopy was performed, and multiple bi-lobar liver lesions were found suggestive of secondary liver involvement of lymphoma. A wedge liver biopsy was performed which revealed granulomatous inflammation of the liver suggesting tuberculosis. Although the Acid-Fast stain was negative the patient was started on anti-TB regimen and the patient is currently asymptomatic and being followed up.</p><p><b><i>Discussion:</i></b> This case report highlights the significance of taking TB into account as a potential cause in patients with lower gastrointestinal symptoms in TB-endemic areas by emphasizing the diagnostic challenges posed by hepatic tuberculosis with isolated liver involvement. To prevent serious complications of abdominal TB, early diagnosis and timely treatment is crucial. It also shows the importance of diagnostic laparoscopy and biopsy in view of early diagnosis.</p><p><b>PP-01-006</b></p><p><b>Clinicopathological features of colorectal cancer: A retrospective cross sectional study from tertiary referral hospital of Karachi</b></p><p>Nazish Butt, <b>Nimrah Mehak</b>, Ghulam Mohiuddin and Ghulam Haider</p><p><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Colorectal cancer (CRC) is a global health problem. It is cancer of colon, appendix and rectum. According to GLOBOCON 2018, It is the 4th most common cancer in the world and the third leading cause of cancer mortality.</p><p><b><i>Objective:</i></b> To evaluate the clinicopathological features of CRC. Method: This was cross-sectional retrospective study conducted at the Gastroenterology Department of JPMC, Karachi from Jan. 2018 till Dec. 2022. All patients with CRC were included in the study. The data obtained was analyzed on the statistical software SPSS version 23.</p><p><b><i>Results:</i></b> Total 1149 patients were enrolled with CRC and 566 patients were analyzed. The most common age group was 40 -60 years of age. The majority of patients were male 382 (64.7% ) Smoking had a strong association 51(9%) were smokers followed by Gutka 21 (3.7%). The common clinical presentation was abdominal pain 255(45% ), Bleeding per Rectum 178(31%), constipation 64( 28%), altered bowel habits 15.5% (n=88), diarrhea 8.3%(n=47), weight loss 64 (11.%). Colonoscopy revealed polypoidal growth in 387 (68.3%) of patients. The most common site of CRC was Rectum followed by Sigmoid colon 219(8.6%) and the least common site was Ascending colon 7(1.2%). Moderately differentiated Adenocarcinoma was the most common histological type found in 468(82.5%).</p><p><b><i>Conclusion:</i></b> CRC is the leading cause of mortality. Most commonly involving the left side of colon. We need more studies to make guidelines for screening and surveillance of CRC in our population.</p><p><b>PP-01-007</b></p><p><b>Frequency and clinical characteristics of inflammatory bowel disease : A multicentre study from Karachi ,Pakistan</b></p><p>Nazish Butt and <b>Omar Idris</b></p><p><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Inflammatory bowel diseases (IBD), including Ulcerative colitis (UC) and Crohn's disease (CD), are chronic gastrointestinal disorders influenced by genetic, environmental, immunological, and microbiological factors. Despite limited epidemiological studies in Pakistan, evidence indicates that IBD is increasingly prevalent in the region.</p><p><b><i>Aims:</i></b> The aim of this study is to determine frequency and multifaceted clinicopathological features of IBD</p><p><b><i>Methods:</i></b> This is an ongoing observational, cross-sectional study which is being conducted at, Jinnah Post Graduate medical Centre and National medical center, Karachi . All adult patients newly diagnosed with IBD were included in the study between January 2023 till date.</p><p><b><i>Results:</i></b> The majority of our patients had UC (214, 97%) with a mean age of 38±18 years, equally prevalent in females 112(51%) and males 107(48%). The highest frequency was among the Sindhi community 38(17%), followed by Pathan 27(12%) and Punjabi 15(6%). A positive family history of IBD was seen in 89 (40%) patients, with 77 (35%) reporting per rectal bleed, and 66 (30%) experiencing abdominal pain and weight loss. Extensive colitis (E3) was present in 130 (54%) patients, severe disease in 120 (54%), with 42 (19%) having arthritis and 27 (12.3%) oral ulcers; 83 (37%) responded to Mesalazine and oral steroids, and 4 (1.8%) were referred to surgery</p><p><b><i>Conclusion:</i></b> Ulcerative colitis, affecting adults aged 15-60 with no gender preference, often presents severely with extensive colitis and common extraintestinal symptoms like arthritis. Prompt diagnosis and early gastroenterologist involvement are crucial to prevent progression to colorectal cancer</p><p><b>PP-01-008</b></p><p><b>Colorectal carcinoma in young patients: Single centre study from Karachi, Pakistan</b></p><p>Nazish Butt and <b>Ghulam Mohiuddin</b> and Nimrah Mehak</p><p><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Colorectal cancer (CRC) is rising among young adults. According to GLOBOCON 2018, It is the 4th most common cancer in the world and the third leading cause of cancer mortality.</p><p><b><i>Objective:</i></b> To evaluate the clinicopathological features of CRC among young adults.</p><p><b><i>Method:</i></b> This was cross-sectional Retrospective study conducted at the Gastroenterology and Oncology Department of JPMC, Karachi from Jan. 2018 till Dec. 2022. All patients with CRC under age 30 were included in the study. Diagnosis was made on Colonoscopy and histopathology confirming CRC. The data obtained was analyzed on the statistical software SPSS version 23. Descriptive statistics were obtained by frequencies and percentages.</p><p><b><i>Results:</i></b> Out of 566 patients of 117 young CRC patients were analyzed. The majority, 67 (57.3%) patients were male and 50 (42.7%) were female. Most common ethnic group were in Urdu speaking 47(40%). Smoking had a strong association 13(9%) were smokers. The common clinical presentation was abdominal pain 64 (53%) constipation 45 (38.5%) and Bleeding per Rectum 42 (36%).</p><p>Colonoscopy revealed polypoidal growth in 79 (67%) of patients followed by ulcerated lesions in 23 (19.7%). The most common site of CRC was Rectum affecting 48 (41%) followed by Sigmoid colon 46 (39.3%) and Ascending colon 7(2.6%). Moderately differentiated Adenocarcinoma was the most common histological type found in 96 (82.5%).</p><p><b><i>Conclusion:</i></b> CRC is on rising among young adults, most commonly involving the left side of the colon. We need more studies to make guidelines for screening and surveillance of CRC in our population.</p><p><b>PP-01-009</b></p><p><b>Clinical and endoscopic features of solitary rectal ulcer syndrome: Multicenter study in Karachi, Pakistan</b></p><p>Nazish Butt<sup>1</sup>, <b>Ghulam Mohiuddin</b><sup>1</sup> and Amanullah Abbasi<sup>2</sup></p><p><sup>1</sup><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan;</i> <sup>2</sup><i>National Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Solitary rectal ulcer syndrome (SRUS) is a chronic benign disorder characterized by the presence of an abnormality of the rectum in persons who have a long history of straining during defecation.</p><p><b><i>Methods:</i></b> This was a retrospective observational longitudinal study, conducted in the department of Gastroenterology at Jinnah Post graduate Medical Center and National Medical Center, Karachi, from 1st March 2017 to till July 2024. All adult patients of either gender diagnosed with SRUS on basis of clinical history and endoscopic features were included in the study. The data obtained was analyzed on the statistical software SPSS version 26.</p><p><b><i>Results:</i></b> There were total 85 patients in our study out of which 49(58%) were female and 36(42%) were male. Mean age was 35 ±17 years. Most common presentation was per rectal bleeding 68(80%). The most common predisposing risk factor was 85 (100%) self-digitation, 51(60%) prolong stay in toilet and rectal prolapse 5 (7.1%). Endoscopically most common finding was of single ulcer 80(95.7%). Histology revealed mucosal ulceration 75(88%) extension of muscular fibers 65 (77%), fibromuscular obliteration of lamina propria 59(70%) and crypt distortion 54 (64.3%). Associated psychological condition among patients were obsessive compulsive disorder 51 (60%) and anxiety disorder 46(52%). Most of the patients were treated through lifestyle modification and biofeedback therapy. Only 4 patients were referred to surgical intervention.</p><p><b><i>Conclusion:</i></b> SRUS is a rare disorder with variable clinical presentation. This study revealed rectal self-digitation and psychological illness was the main risk factor for SRUS.</p><p><b>PP-01-010</b></p><p><b>Spectrum of colon polyps in patients presented to tertiary care hospital: Multicenter study in Karachi, Pakistan</b></p><p>Nazish Butt<sup>1</sup>, <b>Ghulam Mohiuddin</b><sup>1</sup>, Ashfaque Ahmed<sup>2</sup> and Amanullah Abbasi<sup>2</sup></p><p><sup>1</sup><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan;</i> <sup>2</sup><i>National Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Colonic polyps are considered as premalignant conditions. Early detection and endoscopic removal of these precancerous lesions are very effective in reducing the incidence and mortality rate of colorectal cancer.</p><p><b><i>Methods:</i></b> This was a prospective observational study, conducted in the department of Gastroenterology , Jinnah Post graduate Medical Center and National Medical Center, Karachi, from 1st March 2014 July 2024. Patients with either gender of any age were included in the study while patient with history of Colonic resection and pregnant were excluded. Polypectomy was done either by EMR technique, Hot/Cold snare or by Biopsy Forceps.</p><p><b><i>Results:</i></b> There were total 210 patients in our study out of which 129(61%) were male and 81(39 %) were female. Mean age was 42 ±16 years. The most common type on gross examination was of large polyps 115(55.7%).Most commonly located in the sigmoid colon. Most common presentation was abdominal pain 145(69%) followed by per rectal bleed 105 (50%) and Constipation 70 (30%).Most common histological diagnosis were tubular adenoma 90(43%), followed by hyperplastic polyp 38 (18%), juvenile retention polyp 32 (15%), 9 (4.3%) Peutz Jaghers Syndrome and malignant polyps 21(10%).</p><p><b><i>Conclusion:</i></b> Tubular adenoma were found in mostly colonoscopies followed by hyperplastic polyp which can develop into the cancer. Patients with adnominal pain and bleeding per rectum should undergo colonoscopies to rule out polyps.</p><p><b>PP-01-011</b></p><p><b>Inverted colonic diverticula-potential pitfalls in colonoscopy</b></p><p>Tsz Yui Chan</p><p><i>Department Of Surgery, New Territories West Cluster, Hospital Authority, Hong Kong, Hong Kong, Hong Kong</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Inverted colonic diverticula (ICD) are rare colonoscopy findings and occur in approximately 0.7% of people. This could possibly be under reporting due to the lack of awareness and limited cases presented as case reports only. This article aims to evaluate the incidence from a personal series prospective, features of inverted colonic diverticulum and possible methods that can help diagnose ICD.</p><p><b><i>Materials and Methods:</i></b> Patients with colonoscopy findings of ICD were reviewed from 2015 to 2024 in the department of surgery, New Territories West Cluster, Hospital Authority, HKSAR.</p><p><b><i>Results:</i></b> Total 2604 colonoscopies were performed by a single endoscopist in this time period and 5 patients were diagnosed with ICD (0.19%).</p><p>The mean age of the patients was 64.2 years old (ranging from 56 years old to 71 years old), with 3/2 male/female ratio. All (5/5) of the ICD were in the area of multiple colonic diverticula, and 60% (3/5) were located in the right-hemi colon. The features of ICD include elevated sessile ‘bubble wrap like’ appearance with fine concentric folds surrounding the lesion which enhanced with Methylene Blue. Gentle pressure with biopsy forceps can revert ICDs into typical diverticula. ICD cases were also reviewed by using Artificial Intelligence (AI) endoscopy system, sometimes failures in differentiating ICDs from polyps by AI were still encountered.</p><p><b><i>Conclusion:</i></b> ICD is a rare intraluminal lesion that can be misinterpreted as elevated polypoid lesion. Meanwhile endoscopists’ experience may still be the most important factor in diagnosing ICD to prevent serious complications from ‘polypectomy’ for misdiagnosed ICDs.</p><p><b>PP-01-012</b></p><p><b>Optimized endoscopic submucosal dissection for colorectal lesions: A retrospective study of a prospective database</b></p><p><b>Jian Chen</b><sup>1</sup> and Ruihua Shi<sup>1,2</sup></p><p><sup>1</sup><i>Southest University, Nanjing, China;</i> <sup>2</sup><i>Zhongda Hospital affiliated to Southeast University, Nanjing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Early diagnosis and treatment are crucial in reducing related mortality of Colorectal cancer, but technical difficulties have limited the application of Endoscopic Submucosal Dissection (ESD) in Colorectal. Hybrid ESD is a simplified method, but has been reported with low en bloc resection rate and higher recurrence risk. Therefore, a modified method known as Optimized ESD was utilized to treat colorectal lesions.</p><p>The study aimed to evaluate the effectiveness and safety of Optimized ESD in treating colorectal lesions compared to Conventional ESD and Rescue Hybrid ESD.</p><p><b><i>Materials and Methods:</i></b> The study included 277 colorectal mucosal lesions of ≥20 mm size treated with Optimized ESD, Conventional ESD, and Rescue Hybrid ESD. The primary outcomes included operation time and speed, while secondary outcomes included en bloc resection rate, postoperative complications rate, and recurrence rate.</p><p><b><i>Results:</i></b> Optimized ESD was significantly better than Conventional ESD and Rescue Hybrid ESD in terms of operation time and speed(38.39 vs 60.19 vs 51.61min; P<0.001; 17.86 vs 14.68 vs 16.52 mm2/min; P=0.045). Optimized ESD was comparable to Conventional ESD and superior to Rescue Hybrid ESD in terms of en bloc resection rate(92.9% vs 96.3% vs 69.7; P<0.001). There was no significant difference in postoperative complications and local recurrence among the three groups.</p><p><b><i>Conclusion:</i></b> Optimized ESD is a safe and effective technique for treating colorectal lesions, with a fast operation speed, short duration, satisfactory en bloc resection rate and recurrence rate.</p><p><b>PP-01-013</b></p><p><b>Study of colonic stent placement for ileocecal obstruction</b></p><p><b>Hirofumi Chiba</b>, Ryo Moriyama, Ittetsu Akiba, Ko Matsumoto, Rina Torigoe, Tomohiko Amano, Yu Obara, Hideaki Itami, Hirohiko Shinkai, Fumitake Ishiyama and Shoichi Kayaba</p><p><i>Iwate Prefectural Isawa Hospital, Oshu, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colonic stent placement in the ileocecal region is technically challenging, and clinical indications should be carefully considered. With only a few cases reported in the literature, we present the results of stent placement in the ileocecal region at our hospital.</p><p><b><i>Materials and Methods:</i></b> We retrospectively reviewed 14 patients who underwent colonic stent placement for ileocecal obstruction at our hospital between September 2019 and April 2024.</p><p><b><i>Results:</i></b> Among the 14 patients, five were bridged to surgery (BTS) and nine received palliative care. All patients experienced prompt relief of obstruction following stent placement and were able to resume eating. In all the five BTS cases, a stent of φ18 × 60 mm was used. No early complications such as perforation or migration were observed. Late complications included subileus before surgery, necessitating early hospitalization in three patients. The average time to surgery was 35 days, with all procedures performed laparoscopically in one-stage.</p><p>In the nine patients who received palliative care, stents of φ18 × 60 mm were used in four, φ18 × 100 mm in three, and φ18 × 120 mm in two. In one patient, microperforation was observed as an early complication; however, the patient’s condition improved with conservative care. As a late complication, two patients required stent replacement due to re-obstruction. The remaining seven patients experienced no stent-related complications before death.</p><p><b><i>Conclusion:</i></b> Stent placement in the ileocecal region carries a significant risk of reocclusion. Prompt surgical intervention should be considered for patients undergoing BTS, as demonstrated in our patient cohort.</p><p><b>PP-01-014</b></p><p><b>Association between longer cecal intubation time and detection and miss rates of colorectal neoplasms</b></p><p><b>Ji Min Choi</b>, Seon Hee Lim, Yoo Min Han, Jooyoung Lee, Eun Hyo Jin, Ji Yeon Seo and Jung Kim</p><p><i>Seoul National University Hospital, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> A longer cecal intubation time (CIT) occurs during colonoscopy under difficult insertion conditions, which may hinder meticulous mucosal observation. However, whether a longer CIT has detrimental effects on the detection of adenomas remains unclear. We aimed to evaluate the effects of CIT on the detection and miss rate of colorectal neoplasms in asymptomatic subjects.</p><p><b><i>Materials and Methods:</i></b> Healthy examinees who underwent colonoscopy between March and July 2011 and August 2015 and December 2016 were retrospectively included. The primary outcome was the adenoma detection rate (ADR) across CIT quartiles; the secondary outcomes included the mean number of adenomas, advanced ADR (AADR), clinically significant serrated lesion (CSSP) detection, adenoma miss rate (AMR), miss rate of CSSPs and any colorectal neoplasms, and mean number of missed colorectal neoplasms in relation to CIT.</p><p><b><i>Results:</i></b> Overall, 12,402 colonoscopy cases were classified into quartiles based on the CIT. The longer the CIT, the lower the ADR (P<0.001), AADR (P=0.004), and mean number of adenomas (P<0.001). The CSSP detection rate was not associated with CIT. In the follow-up colonoscopy, the AMR increased with increasing CIT (P=0.065). The missed rate of CSSPs (P=0.002) and any colorectal neoplasms (P=0.001) also increased with increasing CIT. In multivariate analysis, CIT was significantly associated with ADR, AADR, and AMR.</p><p><b><i>Conclusion:</i></b> A longer CIT is associated with a lower ADR and higher AMR. Therefore, meticulous inspection is important for high-quality colonoscopies, especially in subjects requiring a longer CIT.</p><p><b>PP-01-015</b></p><p><b>Effect of ethyl acetate fraction from andaliman fruit extract on colorectal cancer cell line HT29</b></p><p><b>Darmadi Darmadi</b>, Imelda Rey, Taufik Sungkar, Ilhamd Ilhamd and Masrul Lubis</p><p><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Me, Medan, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective</i></b>: To evaluate the effect of the ethyl acetate fraction of andaliman (Zanthoxylum acanthopodium) fruit extract on colorectal adenocarcinoma cell line HT29.</p><p><b><i>Materials and methods</i></b>: The extract of andaliman fruit was obtained through maceration of its simplicia. Chemical compound identification and antioxidant testing were performed, continued by assay of cytotoxicity by the MTT method, inhibition of G1 phase and G1-S phase cell cycle using Cyclin D1 and Cyclin E, and apoptosis induction using Caspase 3. These examinations were performed using flow cytometry.</p><p><b><i>Results</i></b>: The IC value from the cytotoxicity test of andaliman fruit was 274,850 μg/mL. Administration of 1IC50 (274.85 μg/ml), ¾IC50 (206.14 μg/ml) from ethyl acetate fraction of andaliman fruit extract could decrease expression of Cyclin D1 or stopped the G1 phase cell cycle and could reduce the expression of cyclin E or inhibit the G1-S phase cell cycle. The IC50 from ethyl acetate fraction of andaliman fruit extract could increase Caspase 3 activity or could trigger apoptosis.</p><p><b><i>Conclusion</i></b>: The ethyl acetate fraction of Andaliman fruit extract has potential as an anticancer agent by reducing cyclin D1, cyclin E expression and having the ability to induce apoptosis by increasing the activity of the Caspase 3 enzyme.</p><p><b><i>Keywords:</i></b> Andaliman, extract, ethyl acetate, colorectal cancer</p><p><b>PP-01-016</b></p><p><b>The recurrence rate of advanced colorectal adenomas after polypectomy: A retrospective cohort study</b></p><p><b>Ma. Regina Dimaculangan</b> and Marianne Linley Sy-Janairo</p><p><i>St. Luke's Medical Center - Global City, Taguig, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colorectal cancer is the third most commonly diagnosed malignancy worldwide. It develops in a multi-step manner from normal epithelium, to a pre-malignant adenoma, and into a malignant carcinoma, hence surveillance colonoscopy is necessary. This study aims to determine the association of high-grade adenoma features and its risk of recurrence. This is critical in early diagnosis and treatment, thereby increasing the chance for good clinical outcomes.</p><p><b><i>Materials and Methods:</i></b> Eligible adult subjects are selected from the pathology data bank, and daily in-hospital census, who have advanced polyps detected during their first colonoscopy, and with at least 2 colonoscopies done from January 2019 to December 2023 in St. Luke's Medical Center - Global City, a tertiary hospital in the Philippines.</p><p><b><i>Results:</i></b> This study included 71 adult patients, with a mean age of 63 years old, and are mostly males. Among the high risk features, the following showed statistically significant p-values that contribute to the recurrence rate of advanced polyps: Family history of colon cancer (HR 1.59, 95% p=0.003), Polyps more than 4mm in size (HR 1.80, p=0.04), More than one high polyp identified (HR 1.26, p=0.04), BPPS with a score 1 (HR 1.78, p=0.03), Tubulovillous morphology (HR 1.59, p=0.03), and Severe dysplasia (HR 1.98, p=0.02). The recurrence years show that the risk is significantly higher within the first 1-2 years post-polypectomy (HR 1.16, p=0.003).</p><p><b>PP-01-017</b></p><p><b>Comparison of in-hospital and home-administration of bowel preparation agent before outpatient colonoscopy in elderly patients</b></p><p><b>Yutaro Fujimura</b><sup>1</sup>, Daisuke Yamaguchi<sup>1,2</sup>, Kasumi Gondo<sup>2</sup>, Naomi Saito<sup>3</sup>, Miwa Takeuchi<sup>3</sup>, Masatoshi Daian<sup>3</sup>, Hitomi Soda<sup>3</sup>, Fumiko Furukawa<sup>3</sup>, Meiko Tonai<sup>3</sup>, Izumi Takamori<sup>3</sup>, Tadahiro Nomura<sup>2</sup>, Shota Fukami<sup>2</sup>, Shunichiro Kimura<sup>2</sup>, Yuichiro Tanaka<sup>2</sup>, Naoyuki Hino<sup>2</sup>, Keisuke Ario<sup>2</sup>, Seiji Tsunada<sup>2</sup>, Ryo Shimoda<sup>1</sup> and Motohiro Esaki<sup>1</sup></p><p><sup>1</sup><i>Division Of Gastroenterology, Department Of Internal Medicine, Faculty Of Medicine, Saga University, Saga City, Japan;</i> <sup>2</sup><i>Department of Gastroenterology, Ureshino Medical Center, Ureshino, Japan;</i> <sup>3</sup><i>Nursing Department, Ureshino Medical Center, Ureshino, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Most patients come to the hospital after taking a bowel preparation agent at home before an outpatient colonoscopy. However, some elderly patients take a bowel preparation agent in the hospital after coming to the hospital for a colonoscopy. This study aimed to compare in-hospital and home use of a bowel preparation agent in elderly patients.</p><p><b><i>Materials and Methods:</i></b> Patients aged 70 years or older who took bowel preparation agent (Moviprep®) before outpatient colonoscopy either in-hospital or at home from September 2022 to September 2023 were prospectively enrolled in Ureshino Medical Center. The primary endpoint was the bowel preparation time before the start of colonoscopy.</p><p><b><i>Results:</i></b> One hundred fifty-one patients (51 in group A and 101 in group B) were enrolled. The mean age was 82.6 ± 5.3 years in Group A and 75.6 ± 3.8 years in Group B (P<0.001). The percentage of patients with ASA-PS:0 was significantly lower in Group A (84.3%) and Group B (98.0%) (P=0.003). The bowel preparation time was 203.9 ± 73.8 minutes in Group A and 270.3 ± 61.5 minutes in Group B (P<0.001), significantly shorter in Group A. Colonoscopy time was similar in both groups (Group A: 29.3 ± 13.7 min vs. Group B: 29.1 ± 12.4 min; P=0.943), and there was no significant difference in adverse events between the two groups.</p><p><b><i>Conclusions:</i></b> In elderly patients with low PS, in-hospital administration of a bowel preparation agent can shorten the bowel preparation time and ensure the same safety as home administration.</p><p><b>PP-01-018</b></p><p><b>Deep neural networks for confirming cecum achievement during screening colonoscopy</b></p><p><b>Sabina Guseinova</b></p><p><i>Yaroslavl Cancer Hospital, Yaroslavl, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Screening colonoscopy is the gold standard for preventing colorectal cancer. An AI approach can be helpful for confirming cecal achievement and monitoring colonoscopy quality.</p><p><b><i>Materials and Methods:</i></b> A digital database was created with 2,671 hand-labeled images from SC collected from more than 250 patients. 2,294 images were negative (without the appendiceal orifice) and 377 were positive (containing the AO). The database was randomly divided into a validation set (80%) and a training set (20%). The validation base had 2,136 images, including 311 positive and 1,825 negative. The training set had 535 images, including 66 positive and 469 negative. For training, we used a ResNet50 convolutional neural network pre-trained on the ImageNet dataset.</p><p><b><i>Results:</i></b> The following results were obtained on a test dataset in the process of the study - the best result on the validation set was AUC = 0.97, and the best value is F1-score = 0.85, when a threshold is th = 0.608. Then the trained model was checked on a test set the area under the curve is equal to AUC = 0.95, F1-score equal 0.9 with a threshold th = 0.462. (table 2) The average analysis time of one image is 29 ms, which allows to process up to 40 images per second.</p><p><b><i>Conclusion:</i></b> We have developed and clinically tested an algorithm based on a DNN for object classification on endoscopic images to confirm cecum achievement with high accuracy. These results can be integrated into a quality control system, reducing subjective medical errors during SC.</p><p><b>PP-01-019</b></p><p><b>Application of automated deep learning for diagnosing high-risk adenomas in colorectal polyps ≤10mm</b></p><p><b>Da Yeon Ham</b>, M.D. Hyun Joo Jang, M.D. Sea Hyub Kae and Jae Gon Lee</p><p><i>Division of Gastroenterology, Department of Internal Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to develop a computer-aided diagnosis (CADx) model using an automated deep learning program to classify low- and high-risk adenomas among colorectal polyps ≤10 mm with standard white-light imaging (WLI) endoscopy.</p><p><b><i>Methods:</i></b> Still images of colorectal adenomas ≤10 mm were extracted. High-risk adenomas were defined as high-grade dysplasia or adenomas with villous histology. Neuro-T version 3.2.1 (Neurocle Inc., Seoul, Korea), an automated deep learning software, was used for deep learning. Accuracy, precision, recall, and F1 score of the deep learning model were calculated. Endoscopy expert and trainee were invited to diagnose endoscopic images to compare their diagnostic accuracy with that of the deep learning models.</p><p><b><i>Results:</i></b> A total of 2,696 endoscopic images (2,460 low-grade and 236 high-grade adenomas) were used for training of the deep learning model. In classifying high- and low-risk adenomas in the validation dataset (323 low-grade and 35 high-grade adenomas), the model demonstrated 95% accuracy, 85.2% precision, 87% recall, and 86.1% F1 score overall. The area under the receiver operating characteristic value for classifying high- and low-risk adenomas was 0.936 and 0.925, respectively. The expert endoscopist and endoscopy trainee showed an overall accuracy of 96.6% and 89.7%, respectively, for discriminating high- and low-risk adenomas in the validation dataset.</p><p><b><i>Conclusion:</i></b> A CADx model established by an automated deep learning program showed high diagnostic performance in differentiating high- and low-risk adenomas among colorectal polyps ≤10 mm using only WLI. The performance of the model was comparable to the expert and superior to the trainee.</p><p><b>PP-01-020</b></p><p><b>The risk stratification of delayed postpolypectomy bleeding in patients with and without chronic kidney disease</b></p><p><b>Hye Kyung Hyun</b>, Hae-Ryong Yun and Cheal Wung Huh</p><p><i>Yonsei University College Of Medicine, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The association between colonoscopic delayed post-polypectomy bleeding (DPPB) and chronic kidney disease (CKD) remains unclear. We aimed to elucidate whether CKD population are at increased risk of DPPB compared with non-CKD population.</p><p><b><i>Methods:</i></b> A large cohort study involved patients who underwent colonoscopy and polypectomy in Korea between 2005 and 2022. We extensively collected various covariates, including patient-related, polyp-related, and procedure-related factors. We conducted large propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) to determine whether CKD affects the occurrence of DPPB. The primary outcome was the association CKD and risk of DPPB, and the secondary outcomes were the risk of DPPB depending on the stage of CKD.</p><p><b><i>Results:</i></b> Among whom 16,885 patients and their respective 41,994 polyps were included in the analysis. Of these, 2,351 individuals (13.9%) were diagnosed with CKD, categorized as follows: 894 (38.0%) in early stage (stages 1,2), 1,457 (68.0%) in advanced stage (stage 3-5); the number of non-CKD population was 14,534. The risk of DPPB in patients with CKD was significantly higher compared to those in the non-CKD group (OR 1.532, CI 1.040-2.257, P=0.031). After large-scale PSM and IPTW, the risk of DPPB was significantly increased according to the stage of CKD (OR 2.375, 95% CI 1.001–5.636 for early stage and OR 2.797, 95% CI 1.201–6.512 for advanced stage, all P<0.05).</p><p><b><i>Conclusions:</i></b> CKD independently increased the risk of DPPB, even in early stages, and was associated with progression to advanced CKD. Careful monitoring during polypectomy is essential at all CKD stages.</p><p><b>PP-01-021</b></p><p><b>Impact of procedure sequence on cecum insertion time in same-day bidirectional endoscopy: Trainee versus expert</b></p><p><b>Hyeong Ho Jo</b>, Eun Young Kim, Joong Goo Kwon, June Hwa Bae, Kang Ho Lee, Jae Jin Lee, Juseok Lee and Gwanghyo Yim</p><p><i>Daegu Catholic University School of Medicine, Daegu, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The optimal sequence for same-day bidirectional endoscopy remains controversial. This study aims to further investigate the impact of procedure sequence on cecum insertion time, with a particular focus on differences between experts (≥10 years) and trainees (<1 year). We hypothesized that abdominal gas insufflation from esophagogastroduodenoscopy (EGD) could impact colonoscopy (CF), specifically prolonging cecal insertion time in trainees.</p><p><b><i>Materials and Methods:</i></b> This retrospective cohort study was conducted at Daegu Catholic University Medical Center from March 2023 to February 2024. A total of 1095 CF cases were included, with 655 performed by experts and 440 by trainees. Among the expert cases, 269 followed EGD first, and 386 followed CF first. In the trainee group, 236 followed EGD first, and 204 followed CF first. Cecum insertion times were compared between these groups.</p><p><b><i>Results:</i></b> In the expert group, the cecum insertion time was 8.6 minutes for the EGD-first group and 8.0 minutes for the CF-first group, indicating no significant difference (p = 0.127). In the trainee group, the cecum insertion time was 13.8 minutes for the EGD-first group and 12.2 minutes for the CF-first group, showing a statistically significant difference (p = 0.011).</p><p><b><i>Conclusion:</i></b> This study demonstrates a significant difference in cecum insertion times between the EGD-first and CF-first procedures in the trainee group but not in the expert group. These findings suggest that performing EGD before CF can increase cecum insertion time for trainees, highlighting the need for protocol considerations in training programs. No significant impact was observed for experienced practitioners.</p><p><b>PP-01-022</b></p><p><b>Meteospasmyl® (alverine citrate plus simethicone) improving the tolerability of bowel preparation for colonoscopy</b></p><p><b>Anastasiia Kasikhina</b>, Sergei Kashin and Dmitrii Zavyalov</p><p><i>Yaroslavl State Cancer Hospital, Yaroslavl, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Colonic dysmotility and spasm are often causing not only difficulties in scope insertion, patient’s discomfort and pain during colonoscopy but also affecting stool transit and patient tolerability during bowel preparation.</p><p>A prospective, single-center, comparative, randomized study was conducted to assess the effect of Meteospasmyl® on the tolerability and effectiveness of preparation for colonoscopy in increased risk of poor-quality bowel cleansing patients. 65 patients aged 32-69 years were randomized in two groups: group I–osmotic sulphate-based laxative Eziсlen® split-regimen preparation (n-32); group II-Eziсlen® split-regimen preparation+Meteospasmyl®(n-33) administered 1 capsule 2 times on the day before colonoscopy and 1 capsule in the morning the day of the exam.</p><p>Spontaneous peristalsis and spasm during colonoscopy prevent the endoscope from moving forward. Spasm aggravates bowel transit, which reduces the quality of preparation and increases the total time of colonoscopy. We found a decrease in the total time of colonoscopy in Meteospasmyl® group (12.4min vs 14.8min (p = 0.04) as well as the time of colonoscope insertion. \"Excellent\" bowel preparation in Meteospasmyl® group was in 43.8%. Bloating during preparation was observed in 12% compared with 3.1% in Meteospasmyl® group(p<0.001). The overall tolerability of preparation using five-point Likert scale was assessed as excellent in 91% of Meteospasmyl® group and in 76% without Meteospasmyl®(p = 0.02).</p><p>Novel approach for overcoming colonic spasm is peroral antispasmodic agent Meteospasmyl®, which improve tolerability of bowel preparation, reduce bowel dysmotility, time of colonoscope insertion and provide high-quality colonoscopy.</p><p><b>PP-01-023</b></p><p><b>Effectiveness of colonoscopy on colorectal cancer incidence among persons 40-49 years</b></p><p><b>Hyun-soo Kim</b> and Su Young Kim and Hyunil Kim</p><p><i>Yonsei Wonju Severance Christian Hospital, Wonju, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aim:</i></b> There are few data on the preventive effect of colonoscopy at ages 40-49 years at the population level. In this study, the risk of CRC according to the presence or absence of CSP in their 40s was evaluated in a retrospective cohort design.</p><p><b><i>Methods:</i></b> Using the Korean National Health Insurance System data between 2004 and 2006, we constructed 1:5 colonoscopy cohort (CsC) and non-colonoscopy cohort (NCsC) at ages 40-49 years and followed through 2020. A total of 2,940,792 subjects were enrolled and CRC risk was evaluated after propensity score matching between CsC and NCsC based on age, sex, smoking, metabolic syndrome (MetS) and potentially relevant variables.</p><p><b><i>Results:</i></b> Among 490,132 CsC subjects and 2,450,660 NCsC subjects at baseline periods in 40-49 ages, 689 (0.14%) and 9,440 (0.39%) cases of CRC were found during 10 years follow up period, respectively. According to age group, cumulative CRC incidences in early 40’s (40-44) were 0.10% in CsC and 0.25% in NCsC respectively. For late 40’s (45-49), those were 0.17% in CsC group and 0.49% in NCsC group, respectively. Compared with NCsC, the adjusted hazard ratios (aHRs) for CRC development in CsC group were 0.42 (men, 95% CI: 0.35-0.50) and 0.37 (women, 95% CI: 0.30-0.47) in early 40’s and 0.33 (men, 95% CI: 0.29-0.37) and 0.39 (women, 95% CI: 0.33-0.45) in late 40’s.</p><p><b><i>Conclusion:</i></b> Overall, colonoscopy in their 40s significantly lowered the risk of colorectal cancer development by 58-67%, suggesting the rationales for optimization of age and gender to start colonoscopy.</p><p><b>PP-01-024</b></p><p><b>A Comparative study of oral sulphate solution and polyethylene glycol in bowel preparation for colonoscopy</b></p><p><b>Ji Liu</b>, Yanjun Chen, Fujuan Luan and Jianying Lu</p><p><i>The First Affiliated Hospital Of Soochow University, Suzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study compared the efficacy of 3 liters of Oral sulfate solution (OSS) and 3 liters of Polyethylene glycol (PEG) in bowel preparation for colonoscopy.</p><p><b><i>Materials and Methods:</i></b> This study retrospectively included 1140 participants who underwent colonoscopy at the First Affiliated Hospital of Soochow University from June 9 to October 26, 2023. Participants were divided into OSS group and PEG group(570 participants per group). The efficacy of bowel preparation was evaluated based on the Boston Bowel Preparation Scale (BBPS).</p><p><b><i>Results:</i></b> There were no significant difference in baseline information between two groups (P>0.05). The bowel preparation success rate was correlated with the type of colonoscopy prep laxatives, gender and diabetes(P<0.05). Bowel preparation success rate in OSS group was higher than that in PEG group (92.3% vs 84.4%, P<0.001). The total BBPS score and scores of each colon segment in OSS group were higher than those in PEG group (P<0.05). There were no difference in bowel movement frequency and adverse reaction incidence rate between both(P >0.05). Additionally, in PEG group,the main factors of the bowel preparation success rate were gender and diabetes,and the bowel preparation success rate of women was 2.025 times that of men (P=0.018),and the bowel preparation success rate in diabetic patients was reduced by 58.8% compared to non-diabetic patients (P= 0.044). There were no significant factors of bowel preparation success rate in OSS group (P > 0.05).</p><p><b><i>Conclusion:</i></b> 3L OSS has more advantages over 3L PEG in bowel preparation for colonoscopy, with a higher bowel preparation success rate.</p><p><b>PP-01-025</b></p><p><b>Explainable AI model for colorectal polyp detection and classification based on wasp and attention mechanism</b></p><p><b>先生 Haoxiang Ni</b></p><p><i>The First Affiliated Hospital of Soochow University, Suzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To develop an interpretable classification model for colorectal lesions to assist endoscopists in learning and clinical decision-making.</p><p><b><i>Materials and Methods:</i></b> We created a Fast RCNN_Resnet50v2_ATT model for detecting and classifying colorectal polyps. The model integrates an attention mechanism to boost precision. It addresses the WASP classification's seven binary questions, synthesizes responses with a Random Forest algorithm for final pathological judgment (HP/AD/SSL), and outputs findings to aid endoscopist comprehension.</p><p>We also trained models using direct pathological typing to emulate non-interpretable models, comparing their accuracy with our model and Suzhou endoscopists on an external test set.</p><p>We utilized the POLAR dataset from Amsterdam for training and validation, applying Chinese endoscopic images for testing.</p><p><b><i>Results:</i></b> The model excels in adenoma with high accuracy (0.89), recall (0.85), and F1 score (0.87). However, it struggles with differentiating hyperplastic polyps and serrated lesions, showing respective scores of 0.55/0.86/0.67 and 0.85/0.56/0.68. These challenges mirror past issues with WASP classification by endoscopists. We plan to refine the model, focusing on the attention module's contribution.</p><p><b>PP-01-026</b></p><p><b>Development of a smartphone application by artificial intelligence to predict bowel preparation quality for colonoscopy</b></p><p><b>Shoko Ono</b><sup>1</sup>, Yuji Ono<sup>2</sup>, Mio Matsumoto<sup>3</sup>, Erina Ishibe<sup>4</sup> and Masaki Inoue<sup>1</sup> and Naoya Sakamoto</p><p><sup>1</sup><i>Hokkaido University Hospital, Sapporo, Japan;</i> <sup>2</sup><i>Sapporo City General Hospital, Sapporo, Japan;</i> <sup>3</sup><i>Sapporo Cancer Screening Center, Public Interest Foundation Hokkaido Cancer Society, Sapporo, Japan;</i> <sup>4</sup><i>Hokkaido University Graduate School of Medicine, Sapporo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The quality of a colonoscopy (CS) is heavily dependent on the quality of bowel preparation. This study aimed to develop an artificial intelligence (AI) system within a cloud computing environment to automatically assess bowel preparation adequacy in real time.</p><p><b><i>Materials and Methods:</i></b> Images of stools taken by patients during preparation for CS were collected and annotated as either complete or incomplete. An AI system was trained on this annotated dataset and integrated into a smartphone application. The application was configured to activate the camera upon scanning a unique QR code assigned to each patient. Patients then photographed the stool in the toilet bowl, and the AI immediately determined the adequacy of the bowel preparation, displaying the result on the screen. The accuracy of the AI system was evaluated using a test set of images and compared with visual judgments made by blinded endoscopists.</p><p><b><i>Results:</i></b> The training set consisted of 107 images with complete preparation and 69 images with incomplete preparation. The test set consisted of 135 images. The application demonstrated an accuracy of 90.4% (95% CI: 84.2−94.3). The sensitivity and specificity were 91% (95% CI: 84.2−94.3) and 89.5% (95% CI: 82.2−94.1), respectively.</p><p><b><i>Conclusion:</i></b> An AI-driven application for the real-time evaluation of bowel preparation quality for colonoscopy has been developed. A clinical trial is currently ongoing to further assess its efficacy.</p><p><b>PP-01-027</b></p><p><b>Investigation for the clinicopathological characteristics of post-colonoscopy of colorectal cancer(PCCRC) at our hospital</b></p><p><b>Motoyuki Onodera</b>, Yuichiro Sato, Takamasa Sato, Masahiro Okada, Rina Kumada, Ryoto Sasaki, Takuya Hara, Hirotaka Ito, Takehiko Igarashi and Yoshitaka Sakai</p><p><i>Osaki Citizen Hospital, Osaki, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The purpose of this study was to investigate the clinicopathological characteristics of PCCRC at our hospital.</p><p><b><i>Methods:</i></b> Among 880 colorectal cancer cases (excluding Tis) diagnosed at our hospital from January 2018 to December 2022, PCCRC was defined as cases detected after endoscopy without colorectal cancer diagnosis within the past 3 years.</p><p><b><i>Results:</i></b> Eight cases of PCCRC were identified during the observation period. The mean age at diagnosis was 72.3 years, and the male-to-female ratio was 6:2. The localization was 2:1:1:0:4:0 in C:A:T:D:S:R. The mean lesion size was 25.3 mm, and the gross appearance were Isp, IIa, IIa+IIc, and IIc+IIa in one case each, and type2 in four cases. The main histological types were tub1 and tub2 in 4 cases each, with mixed por and muc in 1 case each. The depth of the lesions was 2:1:2:2:1 for T1a, T1b, T2, T3, and T4b, and the stage classification was 5:1:2 for I, IIIb, and IV. The mean time from the previous endoscopy to the diagnosis of PCCRC was 24.8 months. Two cases were referred from other hospitals for polyp resection, and multiple polyps were resected. The average colonoscopic withdrawal time was 19.3 minutes, but in 2 cases the withdrawal time was shorter at 3 minutes.</p><p><b><i>Conclusion:</i></b> Some PCCRC cases at our hospital were found in advanced cancers including metastatic lesions. The results suggest that it may be necessary to pay attention to the cases referred for endoscopic treatment, especially involving treatment of multiple lesions, and withdrawal time.</p><p><b>PP-01-028</b></p><p><b>The Effectiveness of artificial intelligence-based colonoscopy: Propensity score matching study</b></p><p><b>Min-suk Park</b><sup>1</sup>, Seung-Jung Yu<sup>1</sup>, Jun-Sik Yoon<sup>1</sup>, Dong-Hoon Baek<sup>2</sup> and Hong-sub Lee<sup>1</sup></p><p><sup>1</sup><i>Inje University Busan Paik Hospital, Busan, South Korea;</i> <sup>2</sup><i>Pusan National University Hospital, Busan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Adenomas, accounting for over 70% of colon cancer origins, make their detection during colonoscopy a critical procedure in colon cancer prevention. Artificial intelligence based on deep learning system has been developed and is under study in countries such as the United States, Japan, and China. This study aims to evaluate the effectiveness of the domestically developed deep learning system-based artificial intelligence, Endoscopy as AI-powered Device (ENAD).</p><p>This study evaluates ENAD's efficacy by retrospectively analyzing 654 colonoscopy cases, excluding 197. Among them, 104 cases used ENAD, and 353 did not. Propensity score matching was used to reduce selection bias.</p><p>Baseline characteristics such as age, gender ratio, Body Mass Index (BMI), obesity ratio, American Society of Anesthesiologists (ASA) score, Boston Bowel Preparation Scale, indications for colonoscopy, and practitioner experience did not significantly differ between the ENAD-assisted and non-assisted groups post-matching. Post-colonoscopy outcomes revealed that the ENAD-assisted group showed higher average polyp detection rates and adenoma detection rates compared to the non-assisted group, although these differences were not statistically significant before or after matching.</p><p>Although the ENAD-assisted group exhibited higher average polyp and adenoma detection rates post-colonoscopy, these differences were not statistically significant. Consequently, the study concludes that ENAD did not significantly improve adenoma or polyp detection rates compared to conventional colonoscopy. Further research with a larger sample size and detailed analysis of medical histories and colonoscopy indications is needed to fully understand the potential benefits of AI-assisted technologies in colon cancer detection and prevention.</p><p><b>PP-01-029</b></p><p><b>A comparison between 1 L polyethylene glycol and oral sodium sulfate tablet for bowel preparation</b></p><p><b>Jin Hwa Park</b><sup>1</sup>, Jeong-Sik Byeon<sup>2</sup> and Su Hyun Park<sup>2</sup></p><p><sup>1</sup><i>Hanyang University, Seoul, South Korea;</i> <sup>2</sup><i>Asan Medical Center, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> A low-volume (1 L) polyethylene glycol plus ascorbic acid (PEG-A) solution and oral sodium sulfate tablet (OST) are recently introduced agents for colonoscopy bowel preparation. This study investigated the efficacy, safety, and tolerability of 1 L PEG-A versus OST.</p><p><b><i>Methods:</i></b> This single-center, prospective, randomized, endoscopist-blinded study randomly assigned patients into 2 groups: 1 L PEG-A (group A); and OST (group B). Efficacy of bowel preparation was assessed using the Boston Bowel Preparation Scale (BBPS). Tolerability and safety were investigated using a standardized questionnaire.</p><p><b><i>Results:</i></b> A total of 174 patients were included in the final analysis (group A, n=92; group B, n=82). Successful bowel preparation was achieved in 91.3% and 95.1% of patients in groups A and B, respectively (p=0.324). Overall mean satisfaction with bowel preparation was greater among those in group B versus vs. those in group A (8.2±1.7 vs. 6.8 ± 2.0, respectively; p<0.001). Although abdominal distention was less common in group A than group B (3/92 [3.3%] vs. 9/82 [11.0%], respectively; p=0.045), overall adverse events developed similarly in both groups (27/92 [29.3%] vs. 21/82 [25.6%], p=0.583). In subgroup analysis of older patients (≥ 65 years of age), efficacy, overall satisfaction, and safety profiles were not different between groups A and B.</p><p><b><i>Conclusions:</i></b> Both 1 L PEG-A and OST demonstrated efficacy, tolerability, and safety for colonoscopy bowel preparation. OST was slightly better tolerated, whereas 1 L PEG-A resulted in less abdominal distention. Both agents were effective and safe in older patients.</p><p><b>PP-01-030</b></p><p><b>Endoscopic stricturotomy of anastomotic strictures following ileo-caecal resection in Crohn’s: First New Zealand Case Series</b></p><p><b>Rajan Patel</b> and Mehul Lamba</p><p><i>Christchurch Hospital, Christchurch, New Zealand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Endoscopic stricturotomy (EST) is a novel endoscopic technique for the management of IBD-related strictures. There are no published reports of this procedure from New Zealand.</p><p><b><i>Materials and Methods:</i></b> We describe the demographics, stricture criteria and early outcomes of 6 cases that underwent EST at a large tertiary hospital in NZ.</p><p><b><i>Results:</i></b> 6 patients with colonoscopy or radiologically confirmed anastomotic strictures at the site of previous ileo-caecal resection for Crohn’s disease have been included. 2 endoscopists performed the procedures. Mean age 49.1 (range 25 to 72), Female 40%. Mean interval between most recent surgery and endoscopic intervention 12.2 years (range 2 to 30 years). No immediate or delayed complications, surgery or hospital admissions at 3 months follow up. No repeat endoscopic interventions were required during the follow up period.</p><p><b><i>Conclusion:</i></b> EST appears to be a safe and efficacious approach to short anastomotic strictures in Crohn’s disease. Longer range follow up and further work to investigate optimised medical management following EST is required.</p><p><b>PP-01-031</b></p><p><b>Bowel preparation quality for colonoscopy - Does distance make the colon grow dirtier?</b></p><p>Callum Hill, Harry White, <b>Rajan Patel</b> and Jonty Morreau</p><p><i>Christchurch Hospital, Christchurch, New Zealand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Aotearoa has an important mix of urban and rural populace resulting in challenging deliverance of healthcare. Increased distance to health services negatively affects patient outcomes, including international literature suggesting poorer bowel preparation quality. We aimed to investigate the effect of distance to an endoscopy unit on the quality of bowel preparation in an Aotearoa population.</p><p><b><i>Materials and Methods:</i></b> A retrospective study was undertaken of the colonoscopies conducted at a large tertiary hospital between August 2023 and October 2023. The distance and expected travel time between patient’s home and endoscopy unit was calculated. Quality of bowel preparation, assessed with the Boston bowel preparation scale (BBPS), at the time of colonoscopy, was recorded. Adequate bowel preparation was defined as BBPS 6 or above. Data was also collected on gender, ethnicity, age, comfort score, indication and number of prior procedures.</p><p><b><i>Results:</i></b> 440 colonoscopies were included in the data analysis. Procedures were stratified into three groups with reference to local geography: urban population (less than 15 minute commute), regional population (15 to 30 minute commute) and rural population (greater than 30 minute commute). The mean commute was 26.9km or 39.1 minutes. Analysis revealed that there was inadequate quality of bowel preparation in 11.8%, 12.4% and 11.8%, respectively.</p><p><b><i>Conclusion:</i></b> About 1 in 10 patients have inadequate bowel preparation. In contrast to international literature, this study suggests there is no negative correlation between distance from endoscopy unit and quality of bowel preparation.</p><p><b>PP-01-032</b></p><p><b>Factor screening to improve the quality of bowel preparation: A prospective single-centre study from China</b></p><p><b>Yu Peng</b> and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To screen the factors that affect the quality of bowel preparation and design a predictive model for improving the quality of bowel preparation.</p><p><b><i>Materials and Methods:</i></b> A total of 1,000 patients who underwent electronic colonoscopy at the Digestive Endoscopy Centre of Xiangya Hospital Central South University were randomly selected. The model was built from 600 consecutive patients (development cohort) who were prospectively scheduled for colonoscopy between September and November 2023. The validation cohort included 400 patients who underwent colonoscopy between December 2023 and January 2024. Bowel preparation was classified as adequate or inadequate. Cleansing was evaluated using the Boston Bowel Preparation Scale (BBPS).</p><p><b><i>Results:</i></b> In the development cohort, BBPS score was adequate in 355 patients (59.2%). In the multivariate analysis, dietary restriction (odds ratio [OR] 2.10, 95% confidence interval [CI] 1.49 –2.96), time interval from the last dose of preparation and colonoscopy (OR 0.40, 95% CI 0.33–0.48), and number of stools after bowel preparation (OR 1.65, 95% CI 1.28 – 2.12) were independent predictors for inadequate cleansing. The model built with these variables showed an area under the curve of 0.76 in the development cohort and 0.70 in the validation cohort. A cut-off of 1.50 predicted inadequate bowel preparation with a sensitivity, specificity, positive predictive value, and negative predictive value of 61.6%, 78.6%, 24.6%, and 94.2%, respectively in the development cohort, 61.2%, 75.1%, 27.3%, and 87.1%, respectively in the validation cohort.</p><p><b><i>Conclusion:</i></b> The model could assist clinicians in predicting which patients are at high risk of inadequate bowel cleanliness.</p><p><b>PP-01-033</b></p><p><b>Factors that affect a patient's decision to undergo anesthetic colonoscopy: Prospective single-center study from China</b></p><p><b>Yu Peng</b> and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To identify the factors that affect a patient’s decision to undergo anesthetic colonoscopy and establish a predictive model to decide whether sedation or analgesia is appropriate before the examination.</p><p><b><i>Materials and Methods:</i></b> A total of 1,000 patients who underwent electronic colonoscopy in the Digestive Endoscopy Center of Xiangya Hospital at Central South University from September to December 2023 were randomly selected. Cleansing was evaluated using the Boston Bowel Preparation Scale (BBPS) by uniformly trained endoscopists. A questionnaire on the factors influencing the choice of anesthetic colonoscopy was completed by all patients after the examination, and a risk model for predicting pain during the colonoscopy was established.</p><p><b><i>Results:</i></b> In the study, 439 patients chose anesthetic colonoscopy. In multivariate analysis, age (odds ratio [OR] 1.45, 95% confidence interval [CI] 1.08 –1.96), the pain tolerance threshold (OR 0.55, 95% CI 0.43 – 0.71), the degree of the worst pain (OR 2.45, 95% CI 1.66 – 3.60), and the duration of pain (OR 9.25, 95% CI 4.58 –18.70) were independent predictors affecting whether patients chose anesthetic colonoscopy. The model built with these variables showed an area under the curve of 0.833 for the cohort, with a sensitivity, specificity, positive predictive value, and negative predictive value of 86.5%, 62.4%, 87.6%, and 56.8%, respectively.</p><p><b><i>Conclusions:</i></b> Patients who are older, have a low pain tolerance threshold, have previously experienced a high degree and longtime of pain in colonoscopy should choose anesthetic colonoscopy.</p><p><b>PP-01-034</b></p><p><b>Argon plasma coagulation in the management of chronic radiation proctitis at Jakarta tertiary referral hospital</b></p><p><b>Adiatmo Pratomo</b></p><p><i>Cipto Mangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>ABSTRACT</p><p><b><i>Introduction:</i></b> Argon Plasma Coagulation (APC) is a non-tactile ablative therapy that helps to stop rectal bleeding in patients who have developed actinic proctitis after exposure to radiotherapy. Chronic Radiation Proctitis (CRP) is a complication that occurs in patients who receive radiation therapy for pelvic malignancies.</p><p><b><i>Objectives:</i></b> This study evaluates the endoscopic features and clinical outcome of patients with Chronic Radiation Proctitis (CRP) after Argon Plasma Coagulation (APC) treatment at Digestive and Pancreatobilioary Endoscopy Department Cipto Mangunkusumo Hospital Jakarta.</p><p><b><i>Materials and Methods:</i></b> The study is a descriptive cross sectional study with total sampling method from secondary data medical record that includes 81 patients who were diagnosed with CRP in the management of APC at Digestive and Pancreatobilioary Endoscopy Department Cipto Mangunkusumo Hospital Jakarta in 2020-2024.</p><p><b><i>Observation:</i></b> The CRP in the management of APC were performed mostly in female patients (81,5%) and at aged range of 34 to 84 years old, with cervix cancer as the main cause of actinic proctitis (87,5%). Control of the bleeding after APC treatment was achieved in 98% of the cases.</p><p><b><i>Conclusion:</i></b> APC treatment was successful in the treatment of CRP patient at Gastroenterology, Pancreatobiliary and Gastrointestinal Endoscopy Department, Cipto Mangunkusumo hospital, Jakarta.</p><p><b><i>Keywords:</i></b> Chronic Radiation Proctitis, Argon Plasma Coagulation</p><p><b>PP-01-035</b></p><p><b>Predictors of adenoma detection rate in colonoscopies for patients with positive fecal immunochemical test</b></p><p><b>R Rajesh</b>, Kimwei Lim, Carlos Paolo Francisco, Christopher Khor Jen Lock and Ravishankar Asokkumar</p><p><i>Singapore General Hospital, SINGAPORE, Singapore</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Adenoma detection rate (ADR) is an established quality measure for colonoscopy. Professional societies recommend an overall ADR of 25% for screening colonoscopy and an overall ADR of 40% for patients who are faecal immunochemical test(FIT) positive. In this study, we evaluated the ADR in our patients who underwent FIT positive colonoscopy(FITC) and identified predictors that increased it.</p><p><b><i>Methods:</i></b> We retrospectively reviewed the records of patients who underwent FITC between November 2016 to November 2020 at a tertiary medical centre in Singapore.</p><p><b><i>Results:</i></b> We analysed 742 patients of which 382 (51%) underwent colonoscopy by gastroenterologists and 360 (49%) by non-gastroenterologists. The mean ±SD age was 66 ±8 years, and majority were females (52%). The mean Boston bowel preparation score was 6.6 ±1.6, the caecal intubation rate was 99%, and the withdrawal time was 14 ±11 minutes. The overall ADR, advanced adenoma detection rate(AADR), and adenoma per colonoscopy (APC) were 57%, 17%, and 1.24 respectively. We found the ADR was significantly higher for gastroenterologists than non-gastroenterologists (69% vs. 44%, p<0.001). Age (OR 1.03), endoscopists' years of experience (OR 2.82), performance of chromoendoscopy (OR 8.18), and withdrawal time (OR 1.22) were significantly predictive of higher ADR. The serrated adenoma and cancer detection rate was 2.8% and 4.6%.</p><p><b><i>Conclusion:</i></b> FITC are held to more stringent quality metrics but this can be surpassed in routine clinical practise with appropriate training and experience. Specifically chromoendoscopy can be an important adjunct in improving adenoma detection in these patients.</p><p><b>PP-01-036</b></p><p><b>Efficacy of endoscopic direct diverticulitis therapy for acute diverticulitis</b></p><p><b>Jianzhen Ren</b>, Silin Huang, Jun Cai, Bo Li, Guang Yang and Xiaolong Xian</p><p><i>Shenzhen University, South China Hospital, Shenzhen, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Acute diverticulitis is mainly treated through medication and surgical intervention. Digital single-operator cholangioscopy (DSOC) has proven effective for managing inflammations in natural conduits such as the bile duct, pancreatic duct, and appendix. This study aimed to investigate the diagnostic and therapeutic value of endoscopic direct diverticulitis therapy (EDDT) of acute diverticulitis.</p><p><b><i>Materials and Methods:</i></b> We performed a retrospective study of patients who underwent EDDT from May 2023 to May 2024 at South China Hospital, Shenzhen University. In the study period, 6 patients with acute diverticulitis underwent EDDT using DSOC. The diagnosis of acute diverticulitis was confirmed by direct colonoscopy imaging and cholangioscope. Fecaliths were meticulously fragmented, extracted, and removed using a disposable basket following repeated lavages with metronidazole and sodium chloride, rendering the mucosa cleansed yet, without evidence of perforation. The success rate of DSOC-assisted EDDT, the procedure time, postoperative length of hospital stay, complications, and recurrence rate were recorded.</p><p><b><i>Results:</i></b> Technical success rate was 100%, with high quality imaging of the diverticular cavity achieved using DSOC in all 6 patients. The mean procedure time was 95.17 (standard deviation [SD] =23.81 ) minutes. All patients experienced immediate relief from abdominal pain after the procedure. Mean postoperative hospitalization was 4.17 (SD=1.17) days. No recurrence occurred during 1–12 months of follow-up.</p><p><b><i>Conclusion:</i></b> EDDT provided a feasible, safe and effective alternative approach for diagnosis and management of acute diverticulitis. This novel approach could reshape the management of acute diverticulitis, emphasizing the importance of technological integration in endoscopic practices.</p><p><b>PP-01-037</b></p><p><b>Inflammatory Cloacogenic Polyps in adolescents with chronic constipation. Can this be endoscopically managed?</b></p><p><b>Jayendra Seetharaman</b>, Arul Premanand Lionel, Leenath Thomas, Sudipta Dhar Chowdury and Amit Kumar Dutta</p><p><i>Christian Medical College, Vellore, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Inflammatory Cloacogenic polyps/polyposis (ICP) are rare kind of polyps arising from anal transitional zone which can spread out in the distal rectum. ICP is one of the components of mucosal prolapse syndromes [1,2]. The diagnosis and management of ICP are poorly defined in literature, especially in the setting of constipation. We present two adolescents with constipation presented with ICP being endoscopically managed.</p><p><b><i>Case report:</i></b> Case 1: 14-years old boy with four years of constipation with the feeling of ineffective evacuation presented with mass descending PR for three months. Colonoscopy showed multiple polypoidal lesions just above the anal verge. Endoscopic mucosal resection done (EMR) for large polypoidal lesion (4 cm) and hot snare polypectomy (HSP) in multiple settings done for the rest. Histopathology is consistent with ICP. 12 months follow-up showed polyp recurrence that was removed by HSP. Anorectal manometry (ARM) revealed type-1 dyssynergia. 6 months follow-up post biofeedback training did not show recurrence. Case 2: 16-years old boy presented with 10 years of constipation with mass descending PR for 2 months. HSP done in 3 sessions for the polypoidal lesions (0.5- 2cms) revealed ICP in histology (Figure 1). Bio-feedback sessions improved the symptoms after ARM showed type-1 dyssynergia. There was no pain or bleeding post-procedure in both the children</p><p><b><i>Conclusions:</i></b> Polyps in the anal verge with inflammatory histology can be ICP. Endoscopic clearance in multiple sessions is required due to its minimal malignant potential. In the setting of constipation, dyssynergia needs to identified and treated to prevent recurrence.</p><p><b>PP-01-038</b></p><p><b>Improvement of colonoscopy insertion technique with the mikoto colonoscopy model: An international multicenter study protocol</b></p><p><b>Ryo Shimoda</b><sup>1</sup>, Daisuke Yamaguchi<sup>2</sup>, Masashi Fujii<sup>3</sup>, Tomohiko Ohya<sup>4</sup>, Naoyuki Tominaga<sup>5</sup>, Hidenori Hidaka<sup>6</sup>, Hiroharu Kawakubo<sup>7</sup>, Yosuke Minoda<sup>8</sup>, Kosuke Maehara<sup>9</sup>, Shoko Imamura<sup>10</sup>, Hiroko Fukuda<sup>11</sup>, Takuma Okamura<sup>12</sup>, Kiwamu Tanaka<sup>13</sup>, Masakuni Kobayashi<sup>4</sup>, Tetsuto Muranaka<sup>14</sup>, Han-Mo Chiu<sup>15</sup>, Shuichi Miyamoto<sup>16</sup>, Mitsuru Esaki<sup>17</sup>, Taro Akashi<sup>18</sup>, Naoyuki Yamaguchi<sup>19</sup>, Yuichiro Ikebuchi<sup>3</sup>, Ayako Takamori<sup>20</sup>, Masaru Ueki<sup>21</sup>, Hajime Isomoto<sup>3</sup> and Motohiro Esaki<sup>2</sup></p><p><sup>1</sup><i>Department Of Endoscopic Diagnostics And Therapeutics, Saga University Hospital, Saga City, Japan;</i> <sup>2</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga City, Japan;</i> <sup>3</sup><i>Department of Gastroenterology and Nephrology, Tottori University Hospital, Yonago City, Japan;</i> <sup>4</sup><i>Department of Endoscopy, The Jikei University School of Medicine, Tokyo, Japan;</i> <sup>5</sup><i>Department of Gastroenterology, Saga-Ken Medical Centre Kosikan, Saga City, Japan;</i> <sup>6</sup><i>Department of Gastroenterology, Saiseikai Karatsu Hospital, Karatsu City, Japan;</i> <sup>7</sup><i>Department of Gastroenterology, Imari Arita Kyouritsu Hospital, Nishimatsuura-gun, Japan;</i> <sup>8</sup><i>Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka City, Japan;</i> <sup>9</sup><i>Department of Gastroenterology, Kitakyushu Municipal Medical Center, Kitakyushu City, Japan;</i> <sup>10</sup><i>Department of Gastroenterology, National Hospital Organization Nagasaki Medical Center, Omura City, Japan;</i> <sup>11</sup><i>Department of Gastroenterology, Sasebo City General Hospital, Sasebo City, Japan;</i> <sup>12</sup><i>Department of Gastroenterology, Nagasaki Harbor Medical Center, Nagasaki City, Japan;</i> <sup>13</sup><i>Department of Gastroenterology, Tottori Prefectural Central Hospital, Tottori City, Japan;</i> <sup>14</sup><i>Department of Gastroenterology, Wakkanai City General Hospital, Wakkanai City, Japan;</i> <sup>15</sup><i>Department of Internal Medicine, National Taiwan University Hospital, Taipei City, Taiwan;</i> <sup>16</sup><i>Division of Endoscopy, Hokkaido University Hospital, Sapporo City, Japan;</i> <sup>17</sup><i>Division of Gastroenterology and Hepatology, Mayo Clinic Arizona, Scottsdale, the United States of America;</i> <sup>18</sup><i>Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Japan;</i> <sup>19</sup><i>Department of Endoscopy and Gastroenterology, Nagasaki University Hospital, Nagasaki City, Japan;</i> <sup>20</sup><i>Department of Clinical Research Center, Saga University Hospital, Saga City, Japan;</i> <sup>21</sup><i>Advanced Medicine Innovation and Clinical Research Center, Tottori University Hospital, Yonago City, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> It is essential for beginning colonoscopists (trainees) to improve the quality of colonoscopy and accelerate their progress, and there are various training methods for this purpose. Furthermore, colonoscopy is more difficult than upper gastrointestinal endoscopy, and colonoscopy insertion training is important for the trainee. This prospective study (imikoto study) was designed with the hypothesis that training using the mikoto colonoscopy model will improve colonoscopy insertion technique in trainees.</p><p><b><i>Materials and Methods:</i></b> This was an international, multicenter, randomized, controlled trial. Twelve trainees with limited colonoscopy experience (<50 cases) were eligible to participate in the study. One trainee per institution will be assumed, and institutions will be assigned to either the study group (mikoto training group) or the standard group (mikoto non-training group) with institution-based randomization (cluster randomization). The primary outcome will be cecal intubation time, and secondary outcomes will include adenoma detection rate (ADR), polyp detection rate (PDR), and ACE tool score. The sample size was calculated to be 35 cases each institution, 420 cases.</p><p><b><i>Results:</i></b> The study is ongoing. (UMIN000054647)</p><p>Conclusions: Our study aims to provide evidence that training with the mikoto colonoscopy model improves colonoscopy insertion technique in trainees.</p><p><b>PP-01-039</b></p><p><b>Applying machine learning to predict bowel preparation adequacy in elderly patients for colonoscopy</b></p><p><b>Dalong Sun</b></p><p><i>Department Of Gastroenterology, Zhongshan Hospital, Fudan University(Xiamen Branch), Xiamen, China;</i> <i>Department Of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to develop and validate a machine learning model to predict bowel preparation adequacy in elderly patients before colonoscopy.</p><p>Materials and Methods: This study targeted elderly patients undergoing colonoscopy, employing the Boruta algorithm for feature selection. Predictive models were constructed using Logistic Regression (LR), Light Gradient Boosting Machines (LightGBM), Support Vector Machines (SVM), Decision Trees (DT), Random Forests (RF), and Extreme Gradient Boosting (XGBoost). Multiple performance metrics were used to compare predictive performance. The SHAP algorithm was utilized to rank feature importance and explain model outputs. A web-based application was developed using the Streamlit framework.</p><p><b><i>Results:</i></b> The study included clinical data from 471 elderly patients with a 23.14% bowel preparation failure rate. The Boruta algorithm identified 7 key features. Among the models constructed, the SVM model performed optimally with an AUC of 0.895 (95% CI: 0.822-0.969), and accuracy, sensitivity, and specificity of 0.889, 0.739, and 0.932, respectively. SHAP analysis revealed significant impacts of these features on model decision processes, with contributions from factors like the last bowel movement being clear liquid, daily bowel movement conditions, and activity levels. The SVM model was transformed into a user-friendly web prediction tool to enhance its practical utility in clinical settings.</p><p><b><i>Conclusion:</i></b> This study successfully developed and validated an interpretable machine learning model for predicting bowel preparation adequacy in elderly patients prior to colonoscopy, culminating in a practical web-based application. This model is expected to accurately identify high-risk patients, allowing for early interventions to significantly improve bowel preparation outcomes.</p><p><b>PP-01-040</b></p><p><b>Investigating the effects of music therapy on patient anxiety during colonoscopy</b></p><p><b>Takuya Takahama</b>, Satoshi Ono, Masahide Ebi, Akane Sugimura, Kazuhiro Yamamoto, Tomoya Sugiyama, Yoshiharu Yamaguchi, Kazunori Adachi, Yasuhiro Tamura, Shinya Izawa, Yasushi Funaki, Naotaka Ogasawara, Makoto Sasaki and Kunio Kasugai</p><p><i>Aichi Medical University Hospital, 1-1 Karimata Yazako Nagakute, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Music therapy has shown promise in reducing anxiety during medical procedures. However, most studies focused on colonoscopies with sedation. We investigated the effects of music therapy on anxiety during colonoscopies performed by same-sex endoscopists without sedation.</p><p><b><i>Methods</i></b>: This was a single-center, randomized controlled trial conducted at Aichi Medical University Hospital between August 2020 and October 2021. We enrolled 160 patients scheduled for colonoscopies and compared their clinical outcomes between music and non-music groups using the Stait-Trait Anxiety Inventory-Form JYZ (STAI) and the Visual Analog Scale.</p><p><b><i>Results</i></b>: Overall, music therapy did not significantly reduce anxiety scores (STAI) compared to the non-music group (p=0.2674). However, female patients and those with a shorter insertion time (<10 minutes) in the music group showed significantly lower STAI scores compared to the non-music group (p=0.0275 and p=0.036, respectively). Additionally, the univariate analysis identified age, presence of polyps, and being a beginner endoscopist as potential risk factors for increased anxiety (p=0.004, 0.043, and 0.049, respectively). Multivariate analysis confirmed the presence of polyps (odds ratio=2.76, 95% confidence interval [CI] 1.2-6.39, p=0.017) and being a beginner endoscopist (odds ratio=2.98, 95%CI 1.21-7.38, p=0.018) were independent risk factors for higher anxiety.</p><p><b><i>Conclusion</i></b>: While music therapy did not universally reduce anxiety during colonoscopy, it may be beneficial for female patients and those experiencing shorter procedures.</p><p><b>PP-01-041</b></p><p><b>Usefulness and safety of new ultrasmall-diameter colonoscope for cases with difficult insertion: A retrospective study</b></p><p><b>Rie Terada</b>, Haruhisa Suzuki, Ryoji Ryoji Ichijima, Kanako Ogura, Akiko Haruta, Yu Takahashi and Hirofumi Kogure</p><p><i>Division Of Gastroenterology And Hepatology, Department Of Medicine, Nihon University School Of Medicine, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colonoscopies are widely available, but there are cases where insertion can be difficult, even for experienced endoscopists. EC-760XP/L (FUJIFILM Corporation, Tokyo, Japan), a new ultrasmall-diameter long scope, may be useful in such cases. The objective of this study is to examine the effectiveness and safety of this new ultrasmall-diameter long scope.</p><p><b><i>Materials and Methods:</i></b> This single-center retrospective study included 39 cases where colonoscope insertion was previously difficult. Endoscope insertion difficulties were defined as cases where cecal intubation could not be achieved by endoscopists of our hospital, or where the cecal intubation time was 15 minutes or longer despite an experienced endoscopist. The primary outcome was the cecum intubation rate using EC-760XP/L compared to a previous examination with a standard scope. The secondary outcomes included the cecal intubation time, sedative use rate, occurrence of adverse events, and pain experience.</p><p><b><i>Results:</i></b> The standard scope achieved cecal intubation in 30 cases (76.9%), whereas EC-760XP/L reached the cecum in all cases (p < 0.01). Cecal intubation times with the EC-760XP/L (9.5 minutes) were significantly shorter compared to the standard scope (19 minutes) (p < 0.01). There was no significant difference in sedative use between the two groups. No adverse events associated with the examination were observed in either group. Pain was observed in 3 cases (8.3%) with the EC-760XP/L, significantly lower than the 22 cases (56.4%) with the standard scope (p < 0.01).</p><p><b><i>Conclusion:</i></b> EC-760XP/L proved to be useful in cases where colonoscope insertion was difficult.</p><p><b>PP-01-043</b></p><p><b>Ultra early needle-knife fistulotomy versus conventional cannulation methods for biliary access during ERCP</b></p><p>João Fernandes<sup>1,2,3</sup>, Rita Ribeiro<sup>1</sup>, Alda Andrade<sup>1</sup>, Isabel Tarrio<sup>1</sup>, Tarcísio Araújo<sup>1</sup>, Patrício Costa<sup>2</sup>, <b>Jorge Canena</b><sup>4,5</sup> and Luís Lopes<sup>1,2</sup></p><p><sup>1</sup><i>ULS Alto Minho, Viana do Castelo, Portugal;</i> <sup>2</sup><i>ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal;</i> <sup>3</sup><i>ULS Cova da Beira, Covilhã, Portugal;</i> <sup>4</sup><i>University Department of Gastroenterology - CUF Tejo Hospital - Nova Medical School, Lisbon, Portugal;</i> <sup>5</sup><i>Amadora Sintra Hospital, Amadora, Portugal</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Primary needle-knife fistulotomy (NKF) was proposed as a superior method for biliary access compared to conventional cannulation methods (CCM), showing higher success rates in biliary cannulation and lower incidence of post-ERCP pancreatitis (PEP), even without specific PEP prevention measures. In clinical practice, endoscopists typically attempt CCM 2-3 times before considering alternative methods. We introduced the concept of ultra-early NKF (UE-NKF), involving one or two attempts lasting less than 2 minutes each before proceeding to NKF. We compared the outcomes of CCM versus UE-NKF in terms of biliary access success and adverse event rates.</p><p><b><i>Materials and Methods:</i></b> A total of 373 patients were prospectively enrolled and randomly assigned to either the CCM or UE-NKF group based on their biliary access technique. We assessed PEP rates, overall adverse events, risk factors, procedural times, and successful cannulation rates between the two groups. All patients received PEP prevention measures.</p><p><b><i>Results:</i></b> Both groups exhibited similar mean risk factors for PEP (CCM 1.33 ± 1.02; UE-NKF 1.31 ± 1.03). Mean cannulation times were 3 minutes and 13 seconds for CCM and 5 minutes and 10 seconds for UE-NKF. The cannulation success rate was 71.36% for CCM compared to 94.25% for UE-NKF (p < 0.001). The overall adverse event rate was 16.90% for CCM versus 13.33% for UE-NKF. PEP occurred in 9.15% of CCM cases and 6.06% of UE-NKF cases (p < 0.01).</p><p><b><i>Conclusion:</i></b> UE-NKF demonstrates significantly higher rates of successful biliary access and lower complication rates, particularly PEP.</p><p><b>PP-01-044</b></p><p><b>Primary needle knife fistulotomy in a large population with different risks of post-ERCP pancreatitis</b></p><p>João Fernandes<sup>1,2,3</sup>, Henrique Coelho<sup>4</sup>, Rita Ribeiro<sup>1</sup>, Fábio Correia<sup>4</sup>, Alda Andrade<sup>1</sup>, Isabel Tarrio<sup>1</sup>, Tarcísio Araújo<sup>1</sup>, Gonçalo Alexandrino<sup>4</sup>, Luís Lourenço<sup>4,5</sup>, David Horta<sup>4,5</sup>, Patrício Costa<sup>2</sup>, Luís Lopes<sup>1,2</sup> and <b>Jorge Canena</b><sup>4,5</sup></p><p><sup>1</sup><i>ULS Alto Minho, Viana do Castelo, Portugal;</i> <sup>2</sup><i>ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal;</i> <sup>3</sup><i>ULS Cova da Beira, Covilhã, Portugal;</i> <sup>4</sup><i>Amadora Sintra Hospital, Amadora, Portugal;</i> <sup>5</sup><i>University Department of Gastroenterology - CUF Tejo Hospital - Nova Medical School, Lisboa, Portugal</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Needle-knife fistulotomy (NKF) as an initial method of cannulation has emerged as a technique associated with a high success rate and virtually no cases of post-ERCP pancreatitis, even in a population at high risk for PEP, without PEP prevention. The objective of this study was to assess the feasibility of performing primary NKF in a large group of consecutive patients with varying risks for PEP.</p><p><b><i>Materials and Methods:</i></b> Multicenter prospective study including all consecutive ERCP patients who underwent primary NKF for biliary access. PEP prevention measures were employed in all cases. We evaluated the success rate, adverse event rate, and PEP rate in the overall population and in subgroups: low risk (0 risk factors for PEP) and high-risk (>1 risk factors). Procedural times and factors associated with PEP were also investigated.</p><p><b><i>Results:</i></b> We included 302 patients (41 with low risk and 251 at high risk, with a mean risk factor of 1.5). The successful biliary cannulation rate was 97.4% (294/302). The mean time to achieve success was 4.54 ± 2.13 minutes. The adverse event rate was 2.6% (n=8), with a PEP rate of 1.65% (n=5) and no severe complications. PEP occurred only in patients with three or more risk factors (p < 0.001).</p><p><b><i>Conclusion:</i></b> Primary NKF is feasible in consecutive patients with a high success rate and a low rate of PEP (although not zero). Having three or more risk factors for PEP increases the risk of PEP when using primary NKF.</p><p>ClinicalTrials.gov ID: NCT06452875</p><p><b>PP-01-044A</b></p><p><b>Recurrence of choledocholithiasis in patients submitted to cholangioscopy-guided lithotripsy-long-term follow-up</b></p><p>Rita Ribeiro<sup>1</sup>, Jorge Canena<sup>2,4</sup>, Tarcísio Araújo<sup>1</sup> and Luís Lopes<sup>1,5</sup></p><p><sup>1</sup><i>ULS Alto Minho, Viana do Castelo;</i> <sup>2</sup><i>University Department of Gastroenterology, CUF Tejo Hospital - Nova Medical;</i> <sup>3</sup><i>School, Lisbon;</i> <sup>4</sup><i>Amadora Sintra Hospital, Amadora;</i> <sup>5</sup><i>ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Cholangioscopy-guided lithotripsy (CGL) is a safe and effective therapy in the treatment of difficult biliary stones. However, there is a lack of information about the long-term recurrence of choledocholithiasis after this technique. This study aimed to evaluate, in the long-term, the possible recurrence of choledocholithiasis in patients with and without gallbladder.</p><p><i><b>Materials and Methods</b>:</i> Single-tertiary center prospective study of all patients submitted to CGL between 2017 and 2023. The recurrence of choledocholithiasis, average time until its occurrence, therapies used during the recurrence, number and size of stones as well as factors associated with recurrence were evaluated.</p><p><b><i>Results:</i></b> CGL was performed in 76 patients (51.3% female), 27 of whom had previously undergone cholecystectomy. The mean number of stones removed was 2, with a mean size of 19.4 mm and 92.1% achieved complete stone removal in the first session, with a 100% succeed rate after the second procedure. The mean follow-up time post CGL was 24 ± 18 months. There was recurrence of choledocholithiasis in 11/76 (14,5%) patients, of which 5 presented with acute cholangitis, with a mean time of 5.5 ± 6.6 months after CGL. In patients with recurrence, 10/11 (90.9%) were female and 8/11 (72.7%) had previous cholecystectomy (both P < 0.01 on multivariate analysis). The mean number of stones removed was 2.4 with a mean size of 21.1 mm. All recurrences were treated with conventional endoscopic techniques.</p><p><b><i>Conclusion:</i></b> CGL is associated with a high success rate and with low rates of choledocholithiasis recurrence during follow-up. Previous cholecystectomy and female gender were independent predictors of choledocholithiasis recurrence.</p><p><b>PP-01-045</b></p><p><b>ERCP to the Rescue: From Iatrogenic Biloma to Recovery</b></p><p><b>Helen Engelina</b>, Tirta Setiawan Tjokroprawiro, Hendra Koncoro and Maria Mayasari</p><p><i>Sint Carolus Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Percutaneous drainage is the treatment of choice for large bilomas. However, it may not always result in complete resolution in some patients. ERCP has emerged as both a diagnostic and therapeutic tool with a high clinical success rate</p><p><b><i>Case:</i></b> A 30-year-old woman presented to the Emergency Unit with right upper abdominal pain, jaundice, and bloating. These symptoms began a month after a laparoscopic cholecystectomy, suggesting a probable iatrogenic bile duct injury leading to bilomas. Physical examination revealed a palpable mass and tenderness in the RUQ. Laboratory results showed anemia (Hb 8.2 g/dL) and elevated direct bilirubin (4.15 mg/dL). A CT scan revealed large cyst-like lesions near the liver and spleen. Percutaneous drainage was performed, which reduced the size but didn't completely resolve the issue, as confirmed by MRCP. ERCP revealed a bile leak (Hannover Classification type C1-C2) in the mid and proximal CBD. Subsequently, stent placement was performed to facilitate the healing of the leak. One day after the ERCP, the patient developed acute pancreatitis, which resolved with supportive treatment. The direct bilirubin level decreased to 1.47 mg/dL. She was discharged and reassessed a week later, reporting no complaints. An upper abdominal ultrasound showed significant reduction of fluid collection in the hepatic region.</p><p><b><i>Discussion:</i></b> Percutaneous drainage of biloma is commonly used as an initial or supplementary approach to provide symptom relief. While ERCP becomes essential to address the underlying issue.</p><p><b><i>Conclusion:</i></b> Combining percutaneous drainage with ERCP enables clinicians to manage bilomas more effectively thereby enhancing patient outcomes.</p><p><b>PP-01-046</b></p><p><b>Treatment Strategies for Pancreatic Stones and Appropriate Timing of Surgical Conversion</b></p><p><b>Yoshiyuki Harada</b>, Arata Sakai and Yuzo Kodama</p><p><i>Kobe University, Kobe, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Minimally invasive endoscopic treatment is commonly used for symptomatic pancreatic stone disease. However, there is no clear consensus on when to transition to surgery if endoscopic procedures are unsuccessful. This study aims to determine the optimal timing for surgical intervention in cases of pancreatic stone disease.</p><p><b><i>Materials and Methods:</i></b> We retrospectively reviewed the outcomes and prognoses of 122 patients who underwent endoscopic treatment for pancreatic stone disease at our hospital between November 2006 and October 2022.</p><p><b><i>Results:</i></b> The median age of the patients was 61 years (range 6-84 years). Of the 122 patients, 77 (72%) underwent ESWL and 11 (9%) EHL, with 97 (80%) achieving complete removal of the pancreatic stones. Surgery was performed in 16 patients (13%). Among these, 2 patients were suspected of having pancreatic cancer and thus underwent surgery. Four patients who had surgery within one year of the initial treatment did not require pancreatectomy. In contrast, among those who had surgery more than one year after the initial treatment, 5 out of 10 patients required pancreatectomy (including 2 cases of PD). Moreover, one patient, who had portal vein obstruction, had difficulty with PD and subsequently underwent gastric jejunal bypass and bile duct jejunal anastomosis.</p><p><b><i>Conclusion:</i></b> While endoscopic treatment for pancreatic stones generally yields favorable results, early surgical intervention should be considered for patients who are difficult to treat endoscopically.</p><p><b>PP-01-047</b></p><p><b>Ice water spray for the prevention of bleeding in precut sphincterotomy</b></p><p><b>Toshinobu Izumi</b> and Mai Sorachi</p><p><i>NHO Himeji Medical Center, Himeji, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Although bleeding is a frequent complication of precut sphincterotomy, it should not be disregarded. Even a small amount of blood makes it difficult to obtain clear endoscopic view of the cutting site, resulting in likelihood of perforation. Ice water spray could cool down the duodenal papilla and cause vasoconstriction that is the same mechanism of hemostasis as epinephrine. We tried to prevent precut bleeding using ice water spray and evaluated efficacy and safety compared with conventional precut.</p><p><b><i>Materials and Methods:</i></b> Between October 2019 and June 2024, 32 patients underwent precut sphincterotomy for biliary access after standard cannulation failed. 18 patients had conventional needle-knife precut by October 2021 and after that, 14 patients underwent ice water spray method. To cool down the papilla, 20ml of ice water was sprayed on the papilla through a needle-knife lumen immediately before starting precut. A small incision of precut was made repeatedly and 5ml of ice water was sprayed every interval of incisions.</p><p><b><i>Results:</i></b> Success rate of biliary access in the ice water spray group and the conventional precut group was 93.0% and 94.4%, respectively. The bleeding rate that required hemostatic procedure including spray or injection of dilute solution of epinephrine and monopolar cautery was lower in the ice water spray group (6.70%) than the conventional precut group (22.2%). No complications of pancreatitis and perforation were found in both the groups.</p><p><b><i>Conclusion:</i></b> Ice water spray during precut sphincterotomy might lower bleeding rate and contribute to performing precut sphincterotomy safely.</p><p><b>PP-01-048</b></p><p><b>Feasibility study of the usefulness using Bullet-type biliary metallic stents</b></p><p><b>Yuki Kawasaki</b></p><p><i>Showa University Koto Tosyosu Hospital, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The usefulness of biliary metallic stents for malignant biliary obstruction is already reported. Metallic stents are thought to provide longer stent patency due to their wider lumen compared to plastic stents. However, metallic stents, due to their wide lumen, increase the risk of retroreflux cholangitis. In the present study, the usefulness of a novel metallic stent (Niti-S○c, Biliary S-type Stent Bullet-type) was evaluated, which may reduce the risk of retroreflux cholangitis by tapering the distal end of the stent.</p><p><b><i>Materials and Methods:</i></b> The technical and clinical success rate, adverse events, recurrent biliary obstruction (RBO) and time to RBO (TRBO) were evaluated in 15 patients who had Bullet-type metal stents placed between April 2023 and June 2024.</p><p><b><i>Results:</i></b> The patient background was a median age of 84 years (50-93) and the primary disease was pancreatic cancer/biliary tract cancer/lymph node metastasis of other cancers 10/3/2. The technical and clinical success rates were 100%. No adverse events, including retroreflux cholangitis, were observed. RBO was observed in one patient (6.7%) and was due to migration. TRBO was 112 days (29-172).</p><p><b><i>Conclusion:</i></b> Due to the short observation period, TRBO is shorter than for previously reported metallic stents, but the RBO rate is lower, and no adverse events have been observed. Bullet-type metallic stents may reduce the risk of retroreflux cholangitis better than conventional metallic stents.</p><p><b>PP-01-049</b></p><p><b>Risk factors of ascending cholangitis developing after endoscopic biliary stenting</b></p><p><b>Raja Taha Yaseen Khan</b> and Hina Ismail and Abbas Ali Tasneem and Nasir Hassan Luck</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ascending cholangitis is associated with increased morbidity and mortality in patients with history of ERCP. We aimed to identify the risk factors of ascending cholangitis following endoscopic biliary stent placement.</p><p><b><i>Materials and Methods:</i></b> In this cross-sectional study, all the patients presenting at hepatobiliary clinic at least two weeks after biliary stenting were included in the study. While, the patients who subsequently found to have cholangitis due to causes e.g. stent occlusion, stent migration, sepsis, urosepsis, pneumosepsis and patients whose biliary drainage was achieved through percutaneous biliary drain (PTBD) placement were excluded. Presence or absence of ascending cholangitis was recorded. Univariate followed by multivariate cox regression analysis was performed to identify independent predictors of ascending cholangitis.</p><p><b><i>Results:</i></b> A total of 128 patients were included in the study. Among them, 70(54.7%) were males. Most common indication for ERCP was CBD stricture noted in 87(68%) patients. Sixty six (51.6%) patients underwent two or more ERCP sessions. Papillotomy was performed in 69(53.9%) patients while sphincteroplasty was done in 38(29.7%) patients. Post ERCP, 53(41.4%) patients had a hospital stay of more than 3 days. Ascending cholangitis developed in 27(21%) patients. The presence of diabetes, prior history of multiple ERCP, papillotomy performed during ERCP and antibiotics given for short duration after ERCP were associated with increased risk of ascending cholangitis.</p><p><b><i>Conclusion:</i></b> The risk factors associated with development of ascending cholangitis were recorded. Prolonged usage of antibiotics after ERCP in patients with these risk factors can decrease the risk of developing ascending cholangitis.</p><p><b>PP-01-050</b></p><p><b>Measurement of sliding force of covered metal stents for migration risk assessment in EUS-guided hepaticogastrostomy</b></p><p><b>Takehiko Koga</b><sup>1</sup>, Hiroshi Yamada<sup>2</sup>, Yusuke Ishida<sup>1</sup>, Naoaki Tsuchiya<sup>1</sup>, Takanori Kitaguchi<sup>1</sup>, Keisuke Matsumoto<sup>1</sup>, Makoto Fukuyama<sup>1</sup> and Fumihito Hirai<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Medicine, Fukuoka University, Fukuoka, Japan;</i> <sup>2</sup><i>Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Migration of covered self-expandable metal stent (CSEMS) during endoscopic ultrasound-guided hepatogastrostomy (EUS-HGS) is a serious complication. The risk of migration is assumed to be influenced by the mechanical properties of the CSEMS, such as radial force (RF) and wire structure (braided or laser-cut types). However, the specific impact of these factors remains unclear. This experimental study aimed to identify the mechanical properties of the CSEMS that influence migration by measuring the sliding force (SF) generated when the CSEMS migrates from the gastric wall.</p><p><b><i>Materials and Methods:</i></b> Seven types of CSEMS were evaluated, including four braided and three laser-cut types. An experimental model using porcine gastric walls and a universal testing machine was used to measure the SF of CSEMS. The CSEMSs were inserted through the porcine gastric wall, and traction was applied to simulate CSEMS migration. The maximum SF (SFmax) for each CSEMS was recorded. Additionally, the RF of each CSEMS was measured, and the correlation between SFmax and RF was analyzed.</p><p><b><i>Results:</i></b> A very strong positive correlation (r>0.9) was found between SFmax and RF. The SFmax values of the laser-cut and braided type CSEMSs exhibited positive and negative residuals, respectively, when compared to the regression line predictions in scatter plots of SFmax and RF.</p><p><b><i>Conclusion:</i></b> The RF of CSEMS is strongly correlated with SF, which together with the wire structure, influences the risk of migration during EUS-HGS.</p><p><b>PP-01-051</b></p><p><b>An unusual case of bowel obstruction following biliary stent migration</b></p><p><b>Chee Lim</b></p><p><i>SCUH, Sunshine Coast, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Endoscopic biliary stenting is a common bridging treatment for difficult to remove choledocholithiasis. 10% of stents migrate, but bowel obstruction from plastic stents is rare.</p><p><b><i>Case Description:</i></b> We present a case of a 80-year-old male presenting with painful jaundice and fevers, on a background of cholelithiasis with intact gallbladder, and low anterior resection for rectal cancer. Workup revealed obstructive liver tests (bilirubin 220umol/L), gram negative bacteremia, and CT showing dilated common bile duct with large choledocholithiasis and an incidental 97x82mm cyst at the appendiceal tip.</p><p>During emergency endoscopic retrograde cholangiopancreatography (ERCP), a 7Fr x 5cm double pigtail stent (DPS) within a partially covered metal stent (SEMS) were deployed due to remnant choledocholithiasis and haemostasis. Despite an initial clinical improvement, patient developed worsening jaundice, vomiting and abdominal pain the next day. DPS was not seen endoscopically and on scout films, so SEMS was replaced with a 7Fr x 7cm DPS after biliary clearance. Follow up CT showed initial DPS lodged proximal to the appendiceal cyst with upstream obstruction. Laparotomy revealed widespread adhesions, and an ileocolic resection was performed to remove the cyst and DPS - histology showed a pT4apN0 appendiceal mucinous neoplasm.</p><p>Patient recovered, and 2 months later underwent laparoscopic cholecystectomy. Subsequent ERCP revealed a clear occlusion cholangiogram after retrieval of remaining DPS.</p><p><b><i>Discussion:</i></b> While distal plastic biliary stent migration is commonly regarded as a fairly benign event, this case highlights the potential for significantly morbid complications in the setting of risk factors for bowel obstruction.</p><p><b>PP-01-052</b></p><p><b>CT Volumetry: A guide for optimal biliary drainage in unresectable malignant hilar biliary obstruction</b></p><p><b>Rishikesh Malokar</b><sup>1</sup>, Shubham Jain<sup>1</sup>, Manisha Joshi<sup>1</sup>, Prasanta Debnath<sup>1</sup>, Sanjay Chandnani<sup>1</sup>, Harsh Gandhi<sup>1</sup>, Jay Chudasama<sup>1</sup>, Sridhar Sundaram<sup>2</sup> and Pravin Rathi<sup>1</sup></p><p><sup>1</sup><i>Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India;</i> <sup>2</sup><i>TATA Memorial Hospital, Mumbai, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Background and Aim:</i></b> Outcomes of drainage in hilar biliary obstruction may depend on the volume of liver accessed and drained. The aim of this study was to determine the effect of draining more than 50% of liver volume on clinical success after intervention.</p><p><b><i>Material and methods</i></b>: Advanced unresectable malignant hilar biliary obstruction (UMHBO) with Bismuth Corlette type II blocks and above were recruited prospectively. Patients underwent CT abdomen, and volumetric analysis was performed manually using TeraReconTM software. Patients were subjected to endoscopic or percutaneous drainage. The primary outcome was clinical success (a fall in baseline bilirubin of ≥50% on day 7), and secondary outcomes were complete drainage, cholangitis, reinterventions, and survival. Patients were categorized into those achieving≥ 50% and <50% drainage groups and outcomes were compared.</p><p><b><i>Results:</i></b> A total of 196 UMHBO were screened, and 80 patients were analyzed after exclusion. Sixty-seven patients (83.75%) underwent ≥ 50% drainage. Clinical success was achieved in 47 (58.75%). Clinical success was significantly higher in the≥ 50% group (OR 3.411; 95% CI, 1.164-9.996; P =0.025), with lesser cholangitis (15%vs 26.3%; P=0.001), reinterventions (12.5%vs23.8%; P=0.001) with improved 90-day survival (58.8% vs 10%; P=0.013). On multivariate analysis, clinical success was a significant predictor against the development of cholangitis, and reinterventions and associated with higher complete drainage.</p><p><b><i>Conclusion</i></b>: Preprocedural CT volumetry may guide the drainage approach with prospective validation of the concept of draining ≥50 % of liver volume. Higher volume drainage leads to better clinical outcomes, lesser cholangitis, reinterventions and 90-day survival.</p><p><b>PP-01-053</b></p><p><b>Pattern of bile cultures and antibiotic sensitivity tests in patients diagnosed with obstructive jaundice</b></p><p><b>Pratiwi Retnaningsih</b></p><p><i>Sebelas Maret University, Sukoharjo, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Background:</i></b> Biliary obstruction causes bacteriobilia and significant morbidity and high mortality, To indentify the characteristics of microbial isolates and from bile cultures taken from obstructive jaundice patients.</p><p><b><i>Methods:</i></b> This was a prospective study conducted between January 2020 and December 2023 at Dr Moewardi Hospital, Surakarta, on 45 patients diagnosed with obstructive jaundice who an ERCP procedure electively. Bile samples were taken intraoperatively, then cultured and tested for antibiotic sensitivity.</p><p><b><i>Result:</i></b> Sixty percent of patients diagnosed with obstructive jaundice who underwent ERCP were women with a ratio of (3:2). Thirty-two percent of patients were between 60 and 75 years old (elderly). The most common cause of obstructive jaundice is gallstones (43% of cases). The dominant isolated pathogenic bacteria in this study was Escherichia coli (40% of cases). This biliary pathogen is sensitive to Amikacin in 40% of cases and meropenem in 40% of cases. Finally, in all patients in this study, biliary pathogenic bacteria were found to be resistant to broad-spectrum antibiotics.</p><p><b><i>Conclusions:</i></b> Microbiological analysis may become a diagnostic as it leads to more accurate selection of antibiotics for the treatment of cholangitis. Selection of empiric antibiotic therapy based on routine bile culture monitoring in patients at high risk of bacteriobilia will potentially help improve outcomes and optimize treatment of acute cholangitis, which is associated with a high mortality rate.</p><p><b><i>Keywords:</i></b> Bile culture, Bacterial Sensitivity Test, Obstructive Jaundice</p><p><b>PP-01-054</b></p><p><b>Precut procedure using FlushKnife for endoscopic minor papilla sphincterotomy</b></p><p><b>Tsuyoshi Sanuki</b>, Seiji Fujigaki, Misaki Yokoi, Katsuhide Tanaka, Yuuta Sumida, Teruhisa Morikawa, Tomomitsu Matono, Atsushi Yamamoto, Kota Tabuchi, Naoki Shinmaru, Makoto Yoshiji and Yoshikazu Kinoshita</p><p><i>Department Of Gastroenterology, Hyogo Prefectural Harima-himeji General Medical Center, Himeji, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Patients of pancreatic divisum may develop recurrent acute pancreatitis (RAP) due to dysfunction of the minor papilla. Therefore, endoscopic minor papilla sphincterotomy is sometimes performed for RAP patients diagnosed by pancreatic divisum. However, some cases are very challenging to insert not only catheter and papillotomy knife but even tip of guidewire to the accessory pancreatic duct (APD) through the poorly formed minor papilla.</p><p><b><i>Case Description:</i></b> We report an 80s female case achieved the cannulation to the APD after the minor papilla precutting procedure using FlushKnife 1.5mm (FUJIFILM, Tokyo, Japan). After cannulation of the APD was obtained, an additional sphincterotomy (Clevercut papillotomy knife; Olympus, Tokyo, Japan) was performed and an endoscopic pancreatic stent was placed and removed after one week to prevent post-ERCP pancreatitis. RAP did not develop 24 months after EMPST.</p><p><b><i>Discussion:</i></b> A precut procedure using a needle electric knife is sometimes performed by an expert endoscopist for difficult biliary cannulation through the main duodenal papilla; however, few cases have reported a precut procedure to cannulate the APD through the minor papilla. We usually perform the precut procedure for the main duodenal papilla in difficult biliary cannulation case using FlushKnife which is usually used for endoscopic submucosal dissection. A characteristic of FlushKnife is that the needle length is short. therefore, precutting procedures for the minor papilla can be performed safely without adverse events. The precut procedure for minor papilla using FlushKnife was useful and safe.</p><p><b>PP-01-055</b></p><p><b>Endoscopic retrograde cholangiopancreatography training model using a silicone simulator using a 3D printing technique</b></p><p><b>Suk Pyo Shin</b><sup>1</sup>, Kyong Joo Lee<sup>2</sup> and Chang-Il Kwon Kwon<sup>1</sup></p><p><sup>1</sup><i>Cha Bundang Medical Center, Seongnam, South Korea;</i> <sup>2</sup><i>Hallym University Dongtan Sacred Heart Hospital, Dongtan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aimed to determine whether endoscopy trainees acquired proficiency in ERCP techniques and assess any improvements in their skill levels from using this ERCP training model.</p><p><b><i>Materials and Methods:</i></b> An ERCP training model was generated using 3D printing techniques, including five distinct interchangeable and transparent ampullar–common bile duct (CBD) modules. A prospective study using this model was conducted with ten trainees. The technical success rate and examination times for duodenoscope insertion and biliary cannulation were evaluated. In addition, the successful plastic-stent insertion rate and trainee satisfaction were measured.</p><p><b><i>Results:</i></b> These training models simulated all steps of the ERCP procedure; fluoroscopic guidance was not required because of the transparency of the ampulla-CBD module. The success rates for duodenoscopy, cannulation, and plastic stent insertion were 94, 100, and 92%, respectively. The mean satisfaction scores for duodenoscope insertion, cannulation, and plastic stent insertion were 4.4, 4.7, and 4.6 on a 5-point scale, respectively. Five attempts decreased the insertion time (R = −0.591, P < 0.001) and cannulation time (R = −0.424, P = 0.002).</p><p><b><i>Conclusion:</i></b> This ERCP-training silicon model is durable, simulates ERCP techniques easily, and helps trainees improve their ERCP techniques.</p><p><b>PP-01-056</b></p><p><b>Lemmel's syndrome: Case report of a successful endoscopic management</b></p><p><b>Dedy Sudrajat</b></p><p><i>Ciputra Hospital Citra Garden City Jakarta, Jakarta, Barat, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Lemmel's syndrome is a rare cause of obstructive jaundice caused by a periampullary duodenal diverticulum compressing common bile duct with resultant bile duct dilatation. We present a 56-year-old man came with worsening heartburn since 2 days before admission. The patient felt bloated, nauseous, and had decreased appetite. Physical examination showed epigastric tenderness and jaundice. Leucocytosis, elevated of transaminase enzymes, amilase, lipase, and bilirubine were detected. MRCP resulted bile stone in CBD, but ERCP found narrowing of distal CBD but didn't found any stone in CBD. A sphincterotomy was performed succesfully. Two month follow up, no recurrence jaundice was observed. Imaging is pivotal to diagnose Lemmel's syndrome accurately. Ultrasound could reveal biliary tract dilatation but cannot identify duodenal diverticula, which could be assessed by CT, MRI or ERCP. MRCP has very low sensitivity in the detection of duodenum diverticula, but is the method of choice to evaluate their relationship with the distal part of the CBD. The initial management is endoscopic retrograde cholangiopancreatography (ERCP), but in case of failure, interventional radiology and surgery can be an option.</p><p><b>PP-01-057</b></p><p><b>The influence of papilla morphology on biliary cannulation: A nationwide multicenter cohort study in Japan</b></p><p><b>Mamoru Takenaka</b> and Koichi Fujita and Shujiro Yazumi and Toru Maruo and Kazuya Matsumoto and Masanori Asada and Hiroko Nebiki and Keiji Hanada and Toshiharu Ueki and Hirofumi Kawamoto</p><p><i>Kindai University, osaka-sayama, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and objectives:</i></b> Although the impact of Vater papilla’s morphology on biliary cannulation has been reported, multicenter, multi-case analyses are limited. This study investigated the nationwide multicenter prospective cohort study in Japan.</p><p><b><i>Methods:</i></b> The study enrolled 3647 patients with naïve Vater papilla undergoing selective biliary cannulation at 35 Japanese centers between April 2017 and March 2018.</p><p>The macroscopic appearance of the Vater papillae was classified as either separate or non-separate, and the length of the oral ridge, presence of diverticulum, and size of the papillae were also assessed to evaluate the impact of each factor on difficult biliary cannulation.</p><p><b><i>Result:</i></b> The rate of the case in which cannulation times required longer than 5 minutes was significantly higher in the non-separated group (55.9(1657/2964) vs 46.0(314/683) %, P<.0001), the long oral protrusions group (58.7(612/1043) vs 52.2(1359/2604) %, P=0.0004) and the periampullary diverticulum non-exist group (55.9(1523/2724) vs 48.3(442/915), P<.0001).</p><p>The rate of the case with unexpected pancreatography was significantly higher in the non-separated group (41.3(1224/2964) vs 30.8(210/683) %, P<.0001), and the long oral protrusions group (42.9(447/1043) vs 37.9(987/2604) %, P=0.0062).</p><p>The rate of the case with unexpected guidewire insertion into the pancreatic duct was significantly higher in the non-separated group (35.5(1053/2964) vs 26.1(178/683) %, P<.0001), the long oral protrusions group (38.1(397/1043) vs 32.0(834/2604) %, P=0.0006) and the periampullary diverticulum non-exist group (35.0 (953/2724) vs 30.1 (275/915) %, P=0.0068).</p><p><b><i>Conclusion:</i></b> In a nationwide multicenter analysis, Vater’s papilla’s morphology apparently influenced the difficulty of biliary cannulation.</p><p><b>PP-01-058</b></p><p><b>Effect of TXI assessments of Vater's papilla morphology on selective biliary cannulation: A multicenter study</b></p><p><b>Masayuki Kurimoto</b> and Mamoru Takenaka and Yuki Tanisaka and Akihiko Yoshida and Hidekazu Tanaka and Tomohiro Fukunaga and Akashi Fujita and Masafumi Mizuide and Shomei Ryozawa and Masatoshi Kuso</p><p><i>Kindai University, osaka-sayama, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> Texture and color enhancement imaging (TXI) is considered superior to white light imaging (WLI) for performing endoscopic assessments of the orifice of the papilla of Vater. This improved ability to evaluate the orifice of the papilla of Vater using TXI may contribute to improved first-attempt cannulation (FAC) rates and reduced procedure times. This multicenter study aimed to determine whether the evaluation of the morphology of the papilla of Vater using TXI improves FAC rates and reduces biliary cannulation and procedure times.</p><p><b><i>Methods:</i></b> Patients with naïve papilla of Vater who underwent selective biliary cannulation between June 2022 and October 2022 were retrospectively analyzed. The primary outcome of this study was the contribution of TXI to FAC.</p><p><b><i>Results:</i></b> Biliary cannulation was performed in 57 and 56 patients using TXI and WLI, respectively. The biliary cannulation success rates with TXI and WLI were 98.3% and 98.2%, respectively (P=1.00). The FAC success rates with TXI and WLI were 45.6% and 25.0%, respectively (P=0.03). The time required for biliary cannulation and total procedure time of the FAC and non-FAC groups were 2 min and 12 min, respectively (P<0.0001) and 30.5 min and 44 min, respectively (P=0.0017). A multivariate linear regression analysis indicated that using TXI and separate-type papilla of Vater was associated with successful FAC.</p><p><b>PP-01-059</b></p><p><b>Development and evaluation of a self-expandable and easily removable biliary hydrogel stent</b></p><p><b>Takayoshi Tsuchiya</b><sup>1</sup>, Yoshiyasu Nagakawa<sup>2</sup>, Ryosuke Tonozuka<sup>1</sup>, Shuntaro Mukai<sup>1</sup>, Yukitoshi Matsunami<sup>1</sup> and Takao Itoi<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Hepatology, Tokyo Medical University, Tokyo, Japan;</i> <sup>2</sup><i>Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The study aimed to develop a novel biliary stent, the \"Hydrogel Stent (HGS),\" which combines the advantages of plastic stents (PS) and metallic stents (MS), offering self-expandability, long-term patency, removability, and replaceability. This was achieved using the swelling properties of polyvinyl alcohol (PVA) hydrogel as the driving force for expansion.</p><p><b><i>Methods:</i></b> Building on previous research, we confirmed that the swelling and mechanical strength of PVA hydrogel can coexist. We further developed a high-strength HGS that initially resembles a PS and self-expands upon absorbing bile, akin to MS. We evaluated the stent's long-term patency and ease of removal through animal experiments.</p><p><b><i>Results:</i></b> In its dry state, the cylindrical PVA gel exhibited flexibility similar to PS. Upon swelling, it expanded approximately 2.0 times in diameter and length, transitioning into an elastic material with a storage modulus and tear strength of 260 ± 20 kPa and 5.9 ± 0.5 N mm-¹, respectively. The stent demonstrated sufficient strength and flexibility for endoscopic delivery and could be removed endoscopically even after softening due to swelling. In vivo experiments on five pigs showed successful stent placement, with two cases of stent dislodgement and one case of successful endoscopic removal after 4 weeks. Post-removal evaluations revealed minimal bacterial adhesion.</p><p><b><i>Conclusion:</i></b> The novel HGS developed using PVA offers promising features for endoscopic biliary stenting, including self-expandability, long-term patency, and easy removability.</p><p><b>PP-01-060</b></p><p><b>Significance of short guide wires in ercp-related procedures in Japan</b></p><p><b>Jun Ushio</b>, Hisaki Kato, Yuki Kawasaki, Kazuya Sumi and Haruhiro Inoue</p><p><i>Showa University Koto-toyosu Hospital Digestive Disease Center, Koto-ku/Toyosu, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In Japan and some Asian countries, long guide wire of 0.025 inch is often used for ERCP. On the other hand, in Western countries, short guide wire of 0.035 inch is mainly used, and therefore, there are differences in the devices used. In recent years, excellent instruments for ERCP have been developed from Asian countries, but they cannot be spread to Western countries without modification. Therefore, we examined whether short guidewires are appropriate for Japanese medicine.</p><p><b><i>Materials and Methods:</i></b> The instruments were 0.035inch, 260cm Acrobat2TM guidewire (Cook Medical) and Fusion OMNI sphincterotome TM for bile duct cannulation, and various types of extraction balloon and monorail basket catheters were used for stone removal. Stone was removed in a single procedure, and the success rate, procedure time, and adverse events of the procedure were evaluated in 32 patients with common bile duct stones who had no previous papillary procedure were included in the study.</p><p><b><i>Results:</i></b> All patients underwent successful bile duct cannulation, but 2 patients had difficulty with bile duct cannulation and underwent pancreatic sphincter pre-cutting. 7 patients had large stones larger than 10 mm, 25 patients had small stones, and 3 patients underwent lithotripsy. All patients were removed stones completely. Total procedure time averaged 8.7 minutes (6-19 minutes). There was one case of mild pancreatitis.</p><p><b><i>Conclusion:</i></b> The success rate and safety of the short guide wire technique were satisfactory. The development of a device compatible with the short guide wire technique would be useful in Japanese medicine.</p><p><b>PP-01-061</b></p><p><b>Intraductal radiofrequency ablation for neoplasms of the major duodenal papilla with intraductal spread. first results</b></p><p><b>Ayubkhan Vagapov</b> and Yury Starkov and Seda Dzhantukhanova and Rodion Zamolodchikov and Amina Badakhova</p><p><i>Vishnevsky National Medical Research Center for Surgery, Moscow, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The introduction of intraductal radiofrequency ablation into clinical practice provides new opportunities for minimally invasive treatment of patients with adenomas of the major duodenal papilla (MDP), including those with extensive extension to the bile ducts. The aim of our study was to evaluate the feasibility, effectiveness and safety of intraductal RFA in the treatment of patients with type III and IV of MDP adenomas with extensive intraductal spread of more than 10 mm.</p><p><b><i>Materials and Methods:</i></b> From 2022 to 2023, 14 patients with adenomas of the MDP with extension to the CBD and/or МPD underwent intraductal radiofrequency ablation (Table 2). The extent of spread to the common bile duct ranged from 10 to 30 mm, to the main pancreatic duct - from 5 to 11 mm.</p><p><b><i>Results:</i></b> Technical success was achieved in all observations. Complications after performing intraductal RFA were noted in 4 cases: in 2 cases, post-manipulation pancreatitis developed, and in another 2 cases, during control cholangioscopy, residual adenomatous growths were revealed, and therefore these patients required a repeat session of intraductal RFA. Technical implementation of stenting of the main pancreatic duct and common bile duct was achieved in all observations.</p><p><b><i>Conclusion:</i></b> The use of intraductal radiofrequency ablation in the treatment of patients with adenomas of the MDP, characterized by extensive spread to the ducts, made it possible in all cases to ensure complete destruction of the intraductal component of the tumor with a lasting clinical effect without the need for highly traumatic surgical intervention.</p><p><b>PP-01-062</b></p><p><b>ERCP vs EUS for primary biliary drainage in malignant distal biliary obstruction: Systematic review andmeta-analysis</b></p><p><b>Praveen Reddy Vasepalli</b>, Zaheer Nabi and Manu Tandon and Nageshwar Reddy</p><p><i>Asian Institute Of Gastroenterology, Hyderabad, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Objectives:</i></b> Endoscopic ultrasound-guided biliary drainage (EUS-BD) is a viable alternative in cases with failed ERCP transpapillary drainage (ERCP-TPD). This systematic review and meta-analysis aims to compare the efficacy and safety of EUS-BD and ERCP-TPD for primary biliary drainage in patients with distal malignant biliary obstruction (DMBO).</p><p><b><i>Methods:</i></b> We searched Embase, PubMed, and Medline databases for studies comparing EUS-BD and ERCP-TPD in DMBO, from inception until September 2023. The primary endpoint was clinical success and secondary endpoints included technical success, procedure duration, and adverse events.</p><p><b><i>Results:</i></b> Eight studies (815 patients, 56.1% male) were included in this analysis. Indications for biliary drainage were pancreatic carcinoma (75.1%), followed by cholangiocarcinoma (10.1%). Clinical success was comparable between EUS-BD and ERCP-TPD groups (OR 1.34, 95%CI 0.75 to 2.40; p=0.32). Technical success was similar between the two groups (OR 2.09, 95%CI 0.83 to 5.25; p=0.12). There was a trend towards fewer adverse events in the EUS-BD group (OR 0.65 [95%CI 0.40, 1.07]; p=0.09), with significantly lower odds of post-procedure pancreatitis (OR 0.17 [95%CI 0.06, 0.50]; p=0.001).</p><p><b><i>Conclusion:</i></b> EUS-BD is comparable to ERCP for primary biliary drainage in DMBO patients with shorter procedural time and a significantly reduced risk of post-procedure pancreatitis.</p><p><b><i>Key words:</i></b> Malignant biliary obstruction; ERCP; Metal stent; EUS</p><p><b>PP-01-063</b></p><p><b>Predictive factors for spontaneous passage of common bile duct stone</b></p><p><b>Wasuwit Wanchaitanawong</b><sup>1</sup>, Phichayut Phinyo<sup>2,3</sup>, Phuripong Kijdamrongthum<sup>1</sup> and Nithi Thinrungroj<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;</i> <sup>2</sup><i>Center for Clinical Epidemiology and Clinical Statistics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;</i> <sup>3</sup><i>Department of Biomedical Informatics and Clinical Epidemiology (BioCE), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To explore predicting factors associated with spontaneous passage of common bile duct stone (CBDS).</p><p><b><i>Materials and Methods:</i></b> A retrospective observational cohort study of patients diagnosed with CBDS from January 2020 to December 2021 at Chiang Mai University Hospital was conducted. The confirmation of spontaneous passage of CBDS was performed by either endoscopic ultrasonography (EUS) or endoscopic retrograde cholangiopancreatography (ERCP).</p><p><b><i>Results:</i></b> Of 172 patients with CBDS, 44 (25.6%) patients had spontaneous passage of CBDS, which was confirmed by ERCP in 34 patients and by EUS in 10 patients. In multivariable analysis, presentation with pancreatitis (RR 1.20, 95% CI 1.01 - 1.43), CBDS size ≤ 3.5 mm (RR 1.41, 95% CI 1.09 - 1.84), single CBDS (RR 1.21, 95% CI 1.08 - 1.35), distal location of CBDS (RR 1.11, 95% CI 1.01 - 1.22), and alkaline phosphatase (ALP) resolution (RR 1.13, 95% CI 1.00 - 1.28) were associated with the likelihood of spontaneous passage of CBDS. There was no significant difference in post-ERCP complications between patients who had spontaneous passage of CBDS and those with non-passage of CBDS (26.5% vs 19.8%, p = 0.479).</p><p><b>PP-01-064</b></p><p><b>Survival benefit of pancreatic stents in patients with pancreatic cancer: a retrospective cohort study</b></p><p><b>Weng Fai Wong</b><sup>1</sup>, Yu Ting Kuo<sup>1</sup>, Ming Lun Han<sup>1</sup> and Hsiu Po Wang<sup>2</sup></p><p><sup>1</sup><i>Department of Integrated Diagnostics & Therapeutics, National Taiwan University Hospital, Taipei, Taiwan;</i> <sup>2</sup><i>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Malnutrition is common in patients with pancreatic ductal adenocarcinoma (PDAC), reducing their tolerance to anti-cancer treatments. Tumor-induced pancreatic duct obstruction blocks the outflow of pancreatic juice, causing maldigestion and poor nutritional status. We aimed to determine if pancreatic stents provide benefits in terms of survival and nutrition for patients with pancreatic cancer.</p><p><b><i>Materials and Methods:</i></b> This retrospective study included patients with PDAC and obstructive jaundice who underwent endoscopic retrograde cholangiopancreatography (ERCP) for biliary stenting between 2007 and 2021 at a medical center in Taiwan. Pancreatic stents were placed following incidental pancreatic duct cannulation for the prevention of post ERCP pancreatitis (PEP), as determined by the endoscopists. Survival and nutritional status were compared between patients with and without pancreatic stents.</p><p><b><i>Results:</i></b> Five hundred and sixty-two patients were enrolled, with 39 patients (7%) having pancreatic stents. The basic characteristics and the proportion of anti-cancer treatments were comparable between the two groups. The incidence of PEP was similar (stent vs. no stent, 5.1% vs. 2.1%, P = 0.227). However, patients with pancreatic stents demonstrated higher severity when pancreatitis occurred (BISAP score, median [IQR], 2.5 [2-3] vs. 1 [1-2], P = 0.045). The pancreatic stent group had shorter overall survival (median [IQR], 170 [95-306] vs. 217 [118-414] days, P = 0.077) and a lower one-year survival rate (HR 1.44, P = 0.048). The nutritional markers revealed no significant difference between the two groups.</p><p><b><i>Conclusion:</i></b> Pancreatic stents provide no benefit in improving nutritional status or prolonging survival in patients with PDAC.</p><p><b>PP-01-065</b></p><p><b>Role of ampullary morphology in assessing difficult biliary cannulation and its impact on ERCP outcomes</b></p><p><b>Raghavendra Yarlagadda</b></p><p><i>American oncology institute/ Citizens hospitals, Hyderabad, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Biliary cannulation involves engaging of accessory with papillary orifice and traversing the intraduodenal part.Role of type of papillary orifice and intraduodenal part of CBD in the successful deep cannulation forms main objective of present study.</p><p><b><i>Material & Methods:</i></b> Retrospective study of prospectively registered ERCPcases for biliary indications was performed.Papillae were classified-normal(Type I),small(Type II),bulging(Type IIIa),pendulous(Type IIIb),creased(Type IV) or peridiverticular(Type D)according to Mohamed etal(modified Haraldsson).Papillary orifice was classified according to Inomata classification,Separate type:isolated(papilla I),gyrate (papilla G)and septal type:Annular(Papilla A),Villous(Papilla V)and unstructured(papilla U).Difficult cannulation is defined as per ESGE guidelines.Any salvage techniques,adverse outcomes following the procedure were recorded according to ASGE-LEXICON.</p><p><b><i>Results:</i></b> 72 naïve papilla from Oct2022-June2024 were included.Malignant etiology were 15,benign cases were 57(cbd stones50,distal CBD stricture4,bile leaks3).Periampullary diverticulum(type D)noted in 7 cases.Papillary type is not significantly associated with etiology.Type II and IIIb papillae and papillary orifice types A and U were associated with significantly longer cannulation times of 4.87(95%CI 2.18-7.32)and 3.11 (95% CI 1.36-7.24)minutes,3.82(95% CI 1.45 – 6.32),4.57(95% CI 3.13 – 7.24)respectively compared to regular papilla(type I) and separate papillary orifices.Type IIIb and U type orifice were associated with lower deep cannulation success.Precut was needed in 3 cases (type A and IIIb).PD stent is placed in 5 cases (septate type)due to inadvertent PD cannulation(typeU 4,typeA 1 and I,II,IV-2,2,1).No difference in cannulation failure between different papillae.Post ERCP pancreatitis in 5cases(typeU and typeII).No difference in the post-ERCP bleeding and infection rates between the different papillae.</p><p><b><i>Conclusions:</i></b> Pendulous papilla(type IIIb)with unstructured(type U)were more difficult to cannulate compared to regular papilla.Identifying papilla type is useful because it may influence the success of cannulation.</p><p><b>PP-01-066</b></p><p><b>Effective combination therapy with full-covered metallic stent and self-assembling peptide for biliary bleeding</b></p><p><b>博士 Dan Zennyoji</b><sup>1</sup>, Takehiro Shimizu<sup>1</sup>, Aoi Kita<sup>1</sup>, Hiroko Hosaka<sup>1</sup>, Yoji Takeuchi<sup>2</sup> and Toshio Uraoka<sup>1</sup></p><p><sup>1</sup><i>Department Of Gastroenterology And Hepatology, Gunma University Graduate School Of Medicine, Showa-machi, Maebashi, Gunma, Japan;</i> <sup>2</sup><i>Department of Endoscopy and Endoscopic Surgery Gunma University Hospital, Showa-machi, Maebashi, Gunma, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Biliary bleeding caused by malignant tumors is difficult to manage, with endoscopic hemostasis using a full-covered metallic stent (FCMS) normally being the treatment of choice. PuraStat® (3-D Matrix, Tokyo), a self-assembling peptide, was developed as an absorbable local hemostatic agent and has been launched in Japan since December 2021 for gastrointestinal endoscopic procedures for oozing bleeding.</p><p><b><i>Case Report:</i></b> A man in his 60s was diagnosed with acute cholangitis due to distal bile duct cancer (T3, N0, M1(HEP), Stage IV), and underwent ENBD. A 10Fr×70mm plastic stent was placed, followed by chemotherapy. Four months after the procedure, the plastic stent was replaced with an 8mm×60mm FCMS. Five months after the replacement, he was hospitalized for cholangitis. ERCP revealed the previous FCMS had dislodged, with continuous bleeding from the tumor. A 10mm×60mm FCMS was placed, however bleeding persisted from the gap between the papilla and the FCMS. PuraStat® (3ml) was applied around the FCMS, achieving hemostasis. After endoscopic hemostasis, no further bleeding was observed until the patient's death two months later</p><p><b><i>Discussion:</i></b> FCMS alone failed to achieve hemostasis, but the application of PuraStat® effectively sealed the bleeding site. PuraStat® is easy to apply as it can be easily combined with other hemostasis methods.</p><p><b>PP-01-067</b></p><p><b>Predictive modeling of 30-day mortality in carbapenem-resistant enterobacterales sepsis Post-ERCP: A retrospective cohort study</b></p><p><b>Hongchen Zhang</b><sup>1</sup>, Xiaochen Zhang<sup>1,2,3</sup>, Hongzhang Shen<sup>1,2,3</sup>, Hangbin Jin<sup>1,2,3</sup>, Jianfeng Yang<sup>1,2,3</sup> and Xiaofeng Zhang<sup>1,2,3</sup></p><p><sup>1</sup><i>Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University, Hangzhou, China;</i> <sup>2</sup><i>Key Laboratory of Integrated Traditional Chinese and Western Medicine for Biliary and Pancreatic Diseases of Zhejiang Province, Hangzhou, China;</i> <sup>3</sup><i>Hangzhou Institute of Digestive Disease, Hangzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to examine the risk factors for 30-day mortality in patients with carbapenem-resistant Enterobacterales (CRE) sepsis following endoscopic retrograde cholangiopancreatography (ERCP) and to develop a nomogram for accurately predicting 30-day mortality risk.</p><p><b><i>Materials and Methods:</i></b> Data from 235 patients who experienced post-ERCP CRE sepsis between January 2005 and December 2022 were analyzed. Variable selection was optimized via the least absolute shrinkage and selection operator (LASSO) regression model. Multivariate logistic regression analysis was then employed to develop a predictive model, which was evaluated in terms of discrimination, calibration, and clinical utility. Internal validation was achieved through bootstrapping.</p><p><b><i>Results:</i></b> The nomogram included predictors such as age >80 years, ICU admission within 90 days prior to ERCP, hypoproteinemia, quick Pitt bacteremia score ≥2, post-ERCP pancreatitis, inappropriate empirical therapy, delayed definitive therapy, and short treatment duration (<10 days). The model demonstrated strong discrimination and calibration.</p><p><b><i>Conclusion:</i></b> This study identified significant risk factors associated with 30-day mortality in patients with post-ERCP CRE sepsis and developed a nomogram to accurately predict this risk. This tool enables healthcare practitioners to provide personalized risk assessments and promptly administer appropriate therapies against CRE, thereby reducing mortality rates.</p><p><b>PP-01-068</b></p><p><b>Safety and necessity of pre-stenting endoscopic sphincterotomy in preventing severe pancreatitis</b></p><p><b>Toru Zuiki</b>, Jun Oki, Takashi Ui, Ns Kaori Nakajima and Ns Mitsuko Sakairi</p><p><i>Yuki Hospital, Yuki, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Endoscopic retrograde biliary drainage (ERBD), involving plastic stent placement post-endoscopic retrograde cholangiopancreatography (ERCP), is crucial for acute suppurative cholangitis or obstructive jaundice due to tumors. However, post-ERCP pancreatitis following ERBD stenting presents a serious concern, attributed to pancreatic duct compression or obstruction by the stent tube, or heightened intrapancreatic duct pressure. In patients with severe pain and hyperamylasemia post-ERBD stenting, immediate stent tube removal prevents severe pancreatitis. Since 2020, procedural modifications included pre-ERBD stenting endoscopic sphincterotomy (EST) to mitigate pancreatic duct compression. The study aims to evaluate whether pre-stenting EST reduces severe post-ERCP pancreatitis.</p><p><b><i>Materials and Methods:</i></b> This retrospective study involved 163 patients (91 men, 72 women) undergoing ERBD stenting for cholangitis or jaundice from January 2016 to May 2024. Outcomes were compared between 57 patients without EST and 106 with EST.</p><p><b><i>Results:</i></b> No EST patients required stent tube removal, whereas three non-EST patients did. Among those requiring stent removal, post-procedure serum amylase levels exceeded 2000 IU/L in three non-EST patients, while none of the EST patients surpassed 1500 IU/L. Neither group experienced gastrointestinal perforation; two EST patients had manageable gastrointestinal bleeding. EST did not significantly affect pain or analgesic use, but no EST patients reported severe pain.</p><p><b><i>Conclusion:</i></b> Pre-stenting EST appears effective in preventing severe post-ERCP pancreatitis without serious complications.</p><p><b>PP-01-069</b></p><p><b>Retractable robotic device for colorectal endoscopic submucosal dissection</b></p><p>Sang Hyun Kim and <b>Hyuk Soon Choi</b></p><p><i>Korea University, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> Appropriate tissue tension and clear visibility of the dissection area using traction are essential for effective and safe endoscopic submucosal dissection (ESD). In this study, we developed a retractable robot-assisted traction device and evaluated its performance in colorectal ESD.</p><p><b><i>Methods:</i></b> An experienced endoscopist performed ESD 18 times on an ex vivo porcine colon using the robot and 18 times using the conventional method. The outcome measures were procedure time, dissection speed, procedure-related adverse events, and blind dissection rate.</p><p><b><i>Results:</i></b> Thirty-six colonic lesions were resected from ex vivo porcine colon samples. The total procedure time was significantly shorter in robot-assisted ESD (RESD) than in conventional ESD (CESD) (20.1±4.1 minutes vs 34.3±8.3 minutes, p<0.05). The submucosal dissection speed was significantly faster in the RESD group than in the CESD group (36.8±9.2 mm2/min vs 18.1±4.7 mm2/min, p<0.05). The blind dissection rate was also significantly lower in the RESD group (12.8%±3.4% vs 35.1%±3.9%, p<0.05). In an in vivo porcine feasibility study, the robotic device was attached to a colonoscope and successfully inserted into the proximal colon without damaging the colonic wall, and ESD was successfully performed.</p><p><b><i>Conclusions:</i></b> The dissection speed and safety profile improved significantly with the retractable RESD. Thus, our robotic device has the potential to provide simple, effective, and safe multidirectional traction during colonic ESD.</p><p><b>PP-01-070</b></p><p><b>Treatment Outcomes of ESD for Rectal pT1 Cancer: Single-center retrospective observational study</b></p><p><b>Yu Ebisawa</b>, HIDEYUKI CHIBA, MASAKI KOGA, Akimichi Hayashi, Jun Arimoto, Hiroki Kuwabara and Michiko Nakaoka</p><p><i>OMORI RED CROSS HOSPITAL, Otaku, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Rectal cancer is suggested to have a higher potential for malignancy and recurrence compared to colon cancer. On the other hand, surgical treatment for lower rectal cancer (Rb cancer) is highly invasive, leading to an increase in diagnostic ESD for lesions suspected of submucosal invasion. However, the safety and efficacy of this approach remains unclear.</p><p><b><i>Material and Methods:</i></b> A retrospective observational study was conducted on cases diagnosed with pT1 rectal cancer from April 2012 to March 2024, among 1,611 lesions treated with colorectal ESD at our hospital. The outcomes of ESD were compared between the Rb group and the non-Rb (Ra or Rs) group.</p><p><b><i>Results:</i></b> Among 37 cases of rectal pT1 cancer, 16 were in the Rb group and 21 in the non-Rb group. Postoperative bleeding occurred in one case in the Rb group. Pathological evaluation showed the depth of invasion (pT1a/pT1b) as 4/12 in the Rb group and 9/12 in the non-Rb group. Only one case in the Rb group had a positive vertical margin. Among the 27 non-curative resections for rectal T1 cancer, 20 cases underwent additional surgical procedures, and lymph node metastasis was found in 4 cases (14.8%).</p><p><b><i>Conclusion:</i></b> Rectal ESD was generally safe. However, for rectal T1 cancer as a whole, there were many non-curative resections. It is suggested that challenging ESD procedures were performed considering the location of the lesions. In this study, there was only one case with a positive vertical margin. However, for accurate pathological diagnosis, complete en bloc resection is expected.</p><p><b>PP-01-071</b></p><p><b>Using a novel hemostatic peptide solution to prevent bleeding after ESD of a gastric tumor</b></p><p><b>Kuniyo Gomi</b>, Erika Yoshida, Misako Tohata and Masatsugu Nagahama</p><p><i>Showa University Fujigaoka Hospital, Yokohama, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Bleeding from an ESD ulcer occurs in 4.4% of post-ESD patients. We wondered if applying PuraStat® (PS) to post-ESD ulcers might reduce post-ESD bleeding. We investigated the preventive potential of PS, a hemostatic formulation, against bleeding in post-ESD gastric ulcers.</p><p><b><i>Materials and Methods:</i></b> From May 2022 to March 2023, 101 patients (Group P) who underwent ESD for gastric diseases at our hospital received PS (2 mL) applied to their post-ESD ulcer. We retrospectively compared this group with a control group (Group C) comprising 297 patients who underwent ESD for gastric diseases at our hospital between April 2017 and March 2021. Post-ESD bleeding was the primary endpoint, while the secondary endpoints included the number of days from ESD to post-ESD bleeding and adverse events associated with PS administration.</p><p><b><i>Results:</i></b> Post-ESD bleeding occurred in 6 (5.9%) (95%CI, 2.8-12.4) and 20 (6.7%) (95%CI, 4.4-10.2) patients in Groups P and C, respectively, with no significant between-group difference. Relative risk was 1.01 (95%CI, 0.95-1.07). No adverse events were observed with PS application. In addition, the median number of days between when ESD was performed and when post-ESD bleeding started was 2 (1–12) and 7.5 (1–14) days in Groups P and C, respectively, with no significant differ between the groups. The lesser curvature or anterior wall was the bleeding site in all 5 patients who experienced postoperative bleeding in Group P.</p><p><b><i>Conclusion:</i></b> PS application is not associated with post-ESD bleeding. However, we infer that gravitational forces affect the effectiveness of applied PS.</p><p><b>PP-01-072</b></p><p><b>Post-ESD wounds closure using mantis clip: Experience with challenging cases</b></p><p><b>Taisuke Inada</b>, Yorinobu Sumida, Nobuyoshi Kodama, Tatsuya Matsumoto, Kousuke Maehara, Shin-ichiro Fukuda and Hirotada Akiho</p><p><i>Kitakyushu Municipal Medical Center, Kitakyushu-city, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Endoscopic submucosal dissection (ESD) is commonly used for gastrointestinal tumors but carries risks such as bleeding and delayed perforation. Effective wound closure after ESD is crucial, yet conventional clips often struggle in areas with thick gastric mucosa and muscle layers. The Mantis clip, with its strong anchoring and gripping ability, may allow for complete wound closure using clips alone. We report a case where the Mantis clip was used in a challenging closure scenario.</p><p><b><i>Case:</i></b> A male in his 60s underwent ESD for an 8 cm type 0-IIc lesion on the greater curvature of the antrum near the pylorus, achieving en bloc resection. Due to the large wound and high bleeding risk, closure was performed using Mantis clips. To prevent stricture formation, clips were placed to create a fold in the muscular layer, and complete closure was achieved with 11 Mantis clips and 9 Sure clips. No bleeding or perforation occurred, and the patient was discharged on day 8 post-treatment without complications. Follow-up endoscopy on day 85 showed complete scar formation with minor narrowing but smooth passage of the endoscope.</p><p><b><i>Discussion:</i></b> The Mantis clip enables complete wound closure in cases where conventional methods are challenging. This technique shows promise in high-risk gastric cases and may prevent post-ESD complications. Further studies comparing its cost-effectiveness and preventive benefits are needed.</p><p><b>PP-01-073</b></p><p><b>Novel insulated tip knife combined with injector can decrease the burden of assistant during procedure</b></p><p><b>Sujin Kim</b> and Jin Ook Jang</p><p><i>Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Procedure of endoscopic submucosal dissection (ESD) is composed of submucosal injection, precut, and dissection. One-step knife (OSK) is a novel knife that combines a knife for ESD and an injection needle into one sheath. In this study, we aimed to evaluate the safety, efficacy, endoscopist and assistant satisfaction of OSK during ESD.</p><p><b><i>Methods:</i></b> We prospective collected medical records of consecutive patients with ESD using OSK from Sep. 2021 to Sep. 2023. We also checked the satisfaction of endoscopists and assistants using questionnaire (5-point scale; 1 very satisfactory, 2 satisfactory, 3 not so satisfactory, 4 dissatisfactory, and 5 very dissatisfactory) about overall procedure and submucosal injection.</p><p><b><i>Results:</i></b> In the study period, a total of 203 patients were analyzed. The total procedure time was 11 min. The procedure time for each location was 14 min in the body, 10 min in antrum. Post-ESD bleeding and perforation rates were 3.9% and 1.0%. Regarding responses to the questionnaire, endoscopists rather than assistants yielded a very satisfactory trend. Rates of very satisfactory or satisfactory were 72% in endoscopists and 87% in assistants.</p><p><b><i>Conclusions:</i></b> OSK can be a feasible knife for gastric ESD. Especially, it could increase the satisfaction of ESD procedure for endoscopists and assistants.</p><p><b>PP-01-074</b></p><p><b>The endoscopic resection for low grade gastric neuroendocrine tumors: A retrospective analysis</b></p><p><b>Wonjun Jang</b> and Cheoul Woong Choi</p><p><i>yangsanpusan university, pusan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The recent surge in screening endoscopy has led to increased detection of gastric neuroendocrine tumors (G-NETs). The selection between radical surgical resection and local excision, including endoscopic resection, for the management of G-NETs remains controversial. Currently, endoscopic resection is recommended for G-NETs < 10 mm. We aimed to evaluate the feasibility of diagnostic endoscopic resection of G-NETs.</p><p><b><i>Methods:</i></b> We retrospectively analyzed 31 patients diagnosed with grade 1 or 2 G-NETs at a single tertiary referral center between January 2009 and December 2023. Outcomes, including histopathology, complete resection, and metastasis rates, were analyzed.</p><p><b><i>Results:</i></b> The mean follow-up period was 38.9 ± 38.4 months. The mean size of G-NET was 4.9 ± 3.4 mm, and most patients’ NETs were <10 mm (87.1%). The maximal NET diameter of NETs was 16 mm. During the study period, most NETs were grade 1 (type 1: 90.9%; type 3: 85.0%). R1 resection patients (19.4%) showed no evidence of metastasis during follow-up without additional surgical management. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Recurrent or multiple G-NETs were observed only in the patients with type 1 NETs (27.2%, 3/11). Modified endoscopic mucosal resection (EMR) (precut, ligation assisted, cap-assisted, and underwater), and endoscopic submucosal dissection (ESD) showed a 100% complete resection rate.</p><p><b><i>Conclusions:</i></b> We observed that G-NETs 1 or 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection is recommended for gastric SETs ≤16 mm in size. Modified EMR or ESD is preferred to conventional EMR.</p><p><b>PP-01-075</b></p><p><b>Vonoprazan or proton-pump inhibitor for gastric endoscopic submucosal dissection in patients under antithrombotic therapy</b></p><p><b>Noboru Kawata</b><sup>1</sup>, Hiroyuki Shibata<sup>2</sup>, Toshihisa Fujiyoshi<sup>3</sup>, Tomohiko Obayashi<sup>4</sup>, Masaya Esaki<sup>5</sup>, Masakazu Kikuchi<sup>6</sup>, Naohiro Yoshida<sup>7</sup>, Tsuguo Kamioka<sup>8</sup>, Yoji Sasaki<sup>9</sup>, Makoto Kobayashi<sup>10</sup>, Kazuhiro Furukawa<sup>2</sup> and Naomi Kakushima<sup>11</sup></p><p><sup>1</sup><i>Division of endoscopy, Shizuoka Cancer Center, Sunto-gun Nagaizumi-cho, Japan;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya city, Japan;</i> <sup>3</sup><i>Department of Gastroenterology, Japanese Red Cross Aichi Medical Center Nagoya Daiichi Hospital, Nagoya, Japan;</i> <sup>4</sup><i>Department of Gastroenterology, Meitetsu Hospital, Nagoya city, Japan;</i> <sup>5</sup><i>Department of Gastroenterology, Handa City Hospital, Handa city, Japan;</i> <sup>6</sup><i>Department of Gastroenterology, Yamashita Hospital, Nagoya city, Japan;</i> <sup>7</sup><i>Department of Gastroenterology, Ishikawa Prefectural Central Hospital, Kanazawa city, Japan;</i> <sup>8</sup><i>Department of Gastroenterology, Kariya Toyota General Hospital, Kariya city, Japan;</i> <sup>9</sup><i>Department of Gastroenterology, Konan Kosei Hospital, Konan city, Japan;</i> <sup>10</sup><i>Department of Gastroenterology, Yokkaichi Municipal Hospital, Yokkaichi city, Japan;</i> <sup>11</sup><i>Department of Endoscopy and Endoscopic Surgery, The University of Tokyo Hospital, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The aim of this study was to evaluate the superiority of vonoprazan (VPZ) over proton pump inhibitor (PPI) in preventing delayed bleeding after gastric endoscopic submucosal dissection (ESD) in patients under antithrombotic therapy.</p><p><b><i>Materials and Methods:</i></b> A multicenter, open-label, randomized controlled trial was conducted at 10 tertiary hospitals in Japan between September 2020 and October 2023. Patients with gastric neoplasms on antithrombotic agents scheduled for ESD were enrolled and randomly allocated in a 1:1 ratio to either the VPZ group (VPZ 20mg once a day) or the PPI group (Esomeprazole 20mg once a day). Antithrombotic agents were managed according to Japanese guidelines, and adjusted factors were institution and interruption of antithrombotic agents. The primary endpoint was the delayed bleeding rate within 8 weeks after ESD, evaluated using intention-to-treat analysis.</p><p><b><i>Results:</i></b> A total of 125 patients were randomized, and 119 patients (median age 78 years, male/female 93/26) were included in this study (59 in the VPZ group and 60 in the PPI group). The delayed bleeding rates were 13.6% (8/59) in the VPZ group and 8.3% (5/60) in the PPI group, with no significant difference (P=0.36). Other adverse events included intraoperative perforation (two in the VPZ group, one in the PPI group), cerebral infarction (one in the VPZ group), drug rash (one in the PPI group), and aspiration pneumonia (one in the PPI group).</p><p><b><i>Conclusion:</i></b> The present study did not demonstrate the superiority of VPZ over PPI in preventing delayed bleeding after gastric ESD in patients under antithrombotic therapy. (UMIN000040641/jRCTs041200045)</p><p><b>PP-01-076</b></p><p><b>A study of forceps resection for small esophageal squamous cell carcinoma</b></p><p><b>Yosuke Kishi</b><sup>1</sup>, Toshiyuki Yoshio<sup>1</sup>, Hiroyuki Yamamoto<sup>1</sup>, Shoichi Yoshimizu<sup>1</sup>, Yusuke Horiuchi<sup>1</sup>, Akiyoshi Ishimaya<sup>1</sup>, Toshiaki Hirasawa<sup>1</sup> and Kaoru Nakano<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan;</i> <sup>2</sup><i>Department of Pathology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> During surveillance endoscopy with iodine staining after endoscopic resection for superficial esophageal cancer, it is common to detect small esophageal squamous cell carcinomas (ESCCs). These ESCCs sometimes disappear upon biopsy as initial diagnosis, however, persistent cases pose challenges in determining the appropriate course of action. At our hospital, we occasionally perform excision using biopsy forceps for these ESCCs. This study aimed to evaluate the efficacy of forceps resection for small ESCCs.</p><p><b><i>Materials and Methods:</i></b> From January 2019 to December 2023, we performed forceps resection on ESCC via endoscopic biopsy forceps at our hospital. We conducted a retrospective analysis on 23 lesions in 21 patients who were followed for more than 6 months.</p><p><b><i>Results:</i></b> The median age was 74 years (range 49-96), with a male to female ratio of 19:4. The median lesion diameter before forceps resection was 4 mm (range 2-7). All patients were diagnosed with squamous cell carcinoma on initial biopsy and had endoscopically diagnosed as cT1a-EP/LPM; none showed signs of deep invasion. The median number of forceps samples taken per lesion was 2 (range 1-4), and the median follow-up period by endoscopy was 19 months (range 6-46). No instances of local recurrence were observed.</p><p><b><i>Conclusion:</i></b> Short-term outcomes of forceps resection for small ESCCs were promising. Although further research is needed, forceps resection appears to be an effective, immediate, and minimally invasive treatment option that does not require hospitalization.</p><p><b>PP-01-077</b></p><p><b>Effective training methods for colorectal endoscopic submucosal dissection (ESD) using outside video and strategy note</b></p><p><b>Mikio Kobayashi</b>, Hideyuki Chiba, Masaki Koga, Akimichi Hayashi, Yu Ebisawa, Jun Arimoto, Hiroki Kuwabara and Michiko Nakaoka</p><p><i>Omori Red Cross Hospital, Ota-ku, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> When a trainee performs ESD at our institution, the trainee is generally instructed under the backup of the supervisor, emphasizing on angle manipulation and stability of the scope operation. We not only take inside but also outside video of endoscopic manipulations during ESD, including the voice of the supervisor providing advice and the sounds of the high-frequency device. After synchronizing inside and outside video, two screens video of the case is made and the trainee can take detailed feedback during review. Before ESD, the trainee also simulates the target lesion in as much detail as possible and prepare strategy note.</p><p><b><i>Materials and Methods:</i></b> 1498 cases of colorectal ESD were performed at our institution from April 2012 to April 2024, and all 1495 cases had en bloc resection except for 3 cases in which treatment was interrupted.</p><p><b><i>Results:</i></b> The resected lesion size was 30 mm (median: 10 mm - 220 mm), and the procedure time was 29 minutes (median: 3-600). 740 cases were performed by 9 trainees (started with ≥30 gastric ESD cases and up to <100 colorectal ESD cases). Trainee's results were excellent with a lesion size of 28 mm (median: 10 mm - 150 mm), a treatment time of 33 minutes (median 7-480), and a self-completion rate of 89.6%.</p><p><b><i>Conclusion:</i></b> The training methods for colorectal ESD at our institution using a combination of outside video and strategy note were safe and effective.</p><p><b>PP-01-078</b></p><p><b>Efficasy of purastat after endoscopic submucosal dissection for gastric neoplasm.</b></p><p><b>Sanshiro Kobayashi</b> and Yuu Takahashi and Tomomitsu Tahara and Naohiro Nakamura and Takuya Shijimaya and Yasushi Matsumoto and Makoto Naganuma</p><p><i>Kansai Medical University, Osaka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Post-ESD bleeding for gastric neoplasm is occurred in about 5%.</p><p>Purastat is a synthetic petptide-based hemostatic agent. Upon contact with acid or bloo, d, it rapidly forms nanofibers and gels, creating p physical barrier at the bleeding site.</p><p>Therefore, we examined the efficacy of Purastat on post-ESD Bleeding.</p><p><b><i>Matrials and Methods:</i></b> We retrospectively compared Purastat group which applied to the post-ESD ulcer of stomach at our hospital from September 2023 to February 2024 and thrombin group from September 2022 to February 2023.</p><p>We defined Post-ESD bleeding cases as decreasing Hb of 2 g/dl or more.</p><p><b><i>Results:</i></b> Purastat/Thrombin group were 63/66 cases. Age 75 (40-90)/75.5 (55-96) years old.</p><p>Procedure time 60 (10-298)/53.5 (10-363) minutes. Tumor resection size 824.3 (175.8-4239)/686.8 (211.6 -5004.3) cm2. Under oral anticoagulantion 18/16 cases.</p><p>Diabetes mellitus 21/10 cases. Median tumor resection size tended to be larger in the PuraStat group. There were significantly more cases of diabetes in the Purestat group. PuraStat group had 2 cases of post-ESD bleeding. One case took aspirin. 4 cases were seen in the thrombin group, one case took apixaban and the other case took clopidogrel.</p><p><b><i>Conclusion:</i></b> Despite the larger resection size and significantly more diabetic patients, there was a trend toward less post-ESD bleeding in the PuraStat group.</p><p><b>PP-01-079</b></p><p><b>Management of intraoperative perforation during colorectal underwater endoscopic submucosal dissection.</b></p><p><b>Shoma Murata</b><sup>1</sup>, Teppei Masunaga<sup>2</sup>, Motoki Sasaki<sup>2</sup>, Yuri Imura<sup>1</sup>, Daisuke Minezaki<sup>2</sup>, Anna Tojo<sup>1</sup>, Hinako Sakurai<sup>1</sup>, Kentaro Iwata<sup>1</sup>, Kurato Miyazaki<sup>1</sup>, Mari Mizutani<sup>2</sup>, Michiko Nishikawa<sup>2</sup>, Teppei Akimoto<sup>2</sup>, Yusaku Takatori<sup>2</sup>, Shintaro Kawasaki<sup>3</sup>, Noriko Matsuura<sup>2</sup>, Hideomi Tomida<sup>2</sup>, Atsushi Nakayama<sup>2</sup>, Tomohisa Sujino<sup>3</sup>, Kaoru Takabayashi<sup>3</sup>, Takanori Kanai<sup>1</sup>, Naohisa Yahagi<sup>2</sup> and Motohiko Kato<sup>3</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;</i> <sup>2</sup><i>Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan;</i> <sup>3</sup><i>Center for Diagnostic and Therapeutic Endoscopy, Keio University School of Medicine, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The underwater technique facilitates the creation of a field of view in colorectal endoscopic submucosal dissection (ESD). Although there are concerns about abdominal pollution with intestinal contents when intraoperative perforation occurs, little is known about the actual clinical course of perforation during colorectal underwater ESD (UESD) in the real world. We aimed to clarify the clinical course of patients with perforations during colorectal UESD.</p><p><b><i>Materials and Methods:</i></b> This was a retrospective observational study. We reviewed cases with intraoperative perforations during colorectal UESD in our institution between June 2020 and May 2024. We evaluated the clinical characteristics, ESD outcomes, management of perforation, and clinical course after ESD.</p><p><b><i>Results:</i></b> Among 534 cases of colorectal UESD, intraoperative perforation occurred in 25 cases (4.7%). The median age was 64 [range, 43−86], the median lesion size was 25 mm [range, 12−80], and the most common location was the ascending colon (30%). En-bloc resection was achieved in 24 cases (96%). Only the perforation site was closed with clips in 15 cases (60%), while the entire wound was completely closed with clips in the remaining cases. After the procedure, fever (>37.5°C) and localized abdominal pain were seen in 8 cases (29%) and 11 cases (46%), respectively. Antibiotics were administered in 21 cases (84%). The median length of hospital stay was 3 days [range,3-9] after colorectal UESD, and there were no cases requiring emergent surgery.</p><p><b><i>Conclusion:</i></b> Intraoperative perforations during colorectal UESD could be controlled only by conservative treatment.</p><p><b>PP-01-080</b></p><p><b>Comparisons cold snare and hot snare endoscopic mucosal resection for intermediate sized colorectal polyps</b></p><p><b>Chang Kyo Oh</b><sup>1</sup>, Young Wook Cho<sup>2</sup>, Jin Bae Kim<sup>1</sup> and Young-Seok Cho<sup>2</sup></p><p><sup>1</sup><i>Hallym University Kangnam Sacred Heart Hospital, Hallym University College Of Medicine, Seoul, South Korea;</i> <sup>2</sup><i>Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> Endoscopic mucosal resection (EMR) is a standard method for the resection of Intermediate sized colorectal polyps. However, several studies are currently conducting on the feasibility of cold snare EMR (CS-EMR) for polyps larger than 10 mm based on the safety of cold resection. Even aside from the cauterization effect, EMR is still widely used because of the benefit of submucosal injection (making the margin of the lesion easier visible and manipulating the lesion into a form that is easier to remove). We aimed to assess the efficacy of CS-EMR, compared with hot snare EMR (HS-EMR), for 10-15 mm sized colorectal polyps.</p><p><b><i>Methods:</i></b> 10-15 mm sized colorectal polyps were randomly allocated to either the CS-EMR group or the HS-EMR group. Primary outcome was recurrence and residual adenoma (RRA) rate.</p><p><b><i>Results:</i></b> A total of 70 and 70 polyps were resected using CS-EMR or HS-EMR, respectively. In the intention-to-treat population, the RRA rate was 1.4% in the CS-EMR group and 1.4% in the HS-EMR group (p=1.000). En bloc resection rate for the CS-EMR and HS-EMR group was 72.9% vs 91.4% (p=0.004) and R0 resection rate was 47.1% vs 78.6% (p=0.001), respectively. Delayed bleeding was 2.9% vs 4.3% (P =0.681) in CS-EMR group and in HS-EMR group, respectively.</p><p><b><i>Conclusion:</i></b> CS-EMR was non-inferior to HS-EMR for the RAA of 10-15 mm sized colorectal polyps. CS-EMR can be considered one of the preferred methods for the removal of 10-15 mm sized benign colorectal polyps.</p><p><b>PP-01-081</b></p><p><b>Usefulness of strip biopsy for small gastric neoplasia</b></p><p><b>Tomomi Sugita</b>, HARUHISA SUZUKI, HIROSHI KINEBUCHI, KEITA KASAMAKI, RIE TERADA, KANAKO OGURA, Yu Takahashi, AKIKO HARUTA and Hirofumi Kogure</p><p><i>Nihon University School Of Medicine, Itabashi-ku, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Endoscopic submucosal dissection (ESD) is a standard treatment for early gastric cancer without lymph node metastasis, but is technically challenging, particularly for lesions in the upper (U) and middle (M) third of the stomach. Strip biopsy, a simpler endoscopic mucosal resection (EMR) method, is used for small gastric neoplasia. This study investigates the efficacy and safety of strip biopsy for small gastric neoplasia.</p><p><b><i>Materials and Methods:</i></b> We retrospectively compared the therapeutic outcomes in 54 patients who underwent ESD (ESD group) and 6 patients who underwent strip biopsy (strip biopsy group) for small gastric lesions (≤15 mm) at our hospital from April 2022 to March 2024.</p><p><b><i>Results:</i></b> The median lesion size was 10 (2-15) mm in the ESD group vs. 4.5 (2-15) mm in the strip biopsy group (p = 0.04). The median resected specimen size was 29.5 (17-40) mm vs. 11 (9-23) mm (p < 0.01). The lesion in U and M/lower stomach was 33/21 vs. 5/1 (p = 0.40). The median procedure time was 43 (10-120) vs. 6.5 (3-8) minutes (p < 0.01). Both groups had a 100% en bloc resection rate. The R0 resection rate was 96.3% vs. 100%, with no significant difference. There were 3 cases of delayed perforation (5.6%) and 0 cases of delayed bleeding in the ESD group, while the strip biopsy group had no such cases.</p><p><b><i>Conclusion:</i></b> Strip biopsy is a useful treatment for small gastric lesions, offering a shorter procedure time without inferior treatment outcomes compared to ESD.</p><p><b>PP-01-082</b></p><p><b>Effective closure of large duodenal esd defect using mantis clip with suction: Case report</b></p><p><b>Yorinobu Sumida</b>, 氏 Nobuyoshi Kodama, Tatsuya Matsumoto, Taisuke Inada, Kosuke Maehara, Shinichiro Fukuda and Hirotada Akiho</p><p><i>Department of gastroenterology, Kitakyushu Municipal Medical Center, Kitakyushu City, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Endoscopic submucosal dissection (ESD) for duodenal tumors is technically challenging and high-risk. Secure closure of the mucosal defect is crucial to prevent delayed perforation. The Mantis clip (Boston Scientific) enables closure of large post-ESD defects with clips alone.</p><p><b><i>Case:</i></b> A 70-year-old male underwent ESD for a duodenal tumor. An 8 cm mucosal defect was closed using Mantis clips. The closure technique involved:1. Water immersion to prevent defect expansion; 2. Long hood attachment with suction; 3. Tissue grasping and inversion with Mantis clips; 4. Closure using 8 Mantis clips and 5 Sure Clips; No postoperative complications occurred. The patient was discharged after one week.</p><p><b><i>Discussion:</i></b> The Mantis clip offers advantages over conventional clips:</p><p>1. Strong grasping power;</p><p>2. Large tissue retention capacity;</p><p>3. Minimal positional shift;</p><p>This technique allows simple procedure completion using clips alone. Combining suction with a long hood enables effective closure of large mucosal defects. Further studies are needed to evaluate its effectiveness in preventing postoperative complications.</p><p><b>PP-01-083</b></p><p><b>Japanese Gastric Cancer Treatment Guidelines can be safely applied for endoscopic resection in the west</b></p><p><b>Edward Young</b><sup>1,2</sup>, Louisa Edwards<sup>2,3</sup>, Andrew Ruszkiewicz<sup>1,4</sup> and Rajvinder Singh<sup>1,2</sup></p><p><sup>1</sup><i>Lyell McEwin Hospital, Northern Adelaide Local Health Network, Adelaide, Australia;</i> <sup>2</sup><i>Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia;</i> <sup>3</sup><i>Royal Adelaide Hospital, Central Adelaide Local Health Network, Adelaide, Australia;</i> <sup>4</sup><i>SA Pathology, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There is considerable disparity in the incidence of gastric cancer between eastern and western countries, with many eastern countries now participating in nationwide gastric cancer screening programs. This has led to the development of risk-stratification models to identify appropriate lesions for endoscopic resection, included in the Japanese Gastric Cancer Treatment Guidelines (2021). We sought to assess whether these guidelines can be safely applied in a western population.</p><p><b><i>Materials and Methods:</i></b> Data was retrospectively recorded for all patients who underwent any form of gastrectomy in four Australian Public Hospitals between 2000 and 2021. Demographic data, lesion characteristics (size, differentiation, invasion depth, lymphovascular invasion and ulceration) as well as the presence and number of lymph node metastases was recorded.</p><p><b><i>Results:</i></b> A total of 1,465 patients were included, 558 of whom underwent gastrectomy for gastric adenocarcinoma (median age 70, 64.2% male). Of these, only 18.4% (n=101, CI 15.4-21.9%) were T1 lesions. Based on the Japanese Gastric Cancer Treatment Guidelines, 11.5% (n=64, CI 9.1-14.4%) met absolute criteria for endoscopic resection, with 7.8% (n=5, CI 3.4-17%) having positive lymph nodes at gastrectomy. According to the eCura system, 9.9% (n=55, CI 7.6-12.6%) would have been considered eCura A or B based on their histology, with none of these having positive lymph nodes at gastrectomy.</p><p><b><i>Conclusion:</i></b> The eCura system for endoscopic curability could have been safely applied in this western population. Even in western countries, patients with early gastric cancer meeting Japanese guidelines for endoscopic resection should where possible undergo en bloc endoscopic resection.</p><p><b>PP-01-084</b></p><p><b>EG-840TP with a dedicated hood can safely resect an early esophageal cancer with esophageal stricture</b></p><p><b>Hiroki Hayashi</b>, Kazuaki Akahoshi, Takashi Ueno, Yoshie Nomoto, Hisashi Fukuda, Haruo Takahashi, Yuji Ino and Hironori Yamamoto</p><p><i>Jichi Medical University, Shimotsuke, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The EG-840TP (Fujifilm Co, Tokyo, Japan) has a tip diameter of 7.9 mm, which is thinner than conventional therapeutic endoscopes. We have used it for endoscopic procedures with a dedicated tapered hood with a 4 mm tip. We present a case in which ESD was successfully completed using these devices in a patient with early esophageal cancer and cervical esophageal stricture.</p><p><b><i>Case report:</i></b> The patient is a 75-year-old man with cervical esophageal stricture caused by chemoradiotherapy for the right pyriform sinus carcinoma. An EGD was performed to screen for metachronous cancer, using an ultrathin 5.8 mm tip diameter endoscope due to the stricture. There was a semi-circumferential 0-IIb lesion in the mid-thoracic esophagus, which was suspicious for squamous cell carcinoma on biopsy. We planned ESD for this lesion and the EG-840TP was inserted with the dedicated hood. The tapered side of the hood wedged into the stricture and bougie dilatation was performed without problems. We were able to reach the lesion and complete the resection by ESD. The pathology results showed a curative resection.</p><p><b><i>Discussion:</i></b> Endoscopic treatment of a lesion located beyond a stricture requires ingenuity. Balloon dilation of the stricture carries a risk of bleeding and perforation. Bleeding makes ESD difficult due to poor visualization. Although it is possible to perform the procedure with an ultrathin endoscope, the small channel diameter limits the devices available. The approach using the thin therapeutic endoscope and the dedicated hood is useful for lesions on the deeper side of a stricture.</p><p><b>PP-01-085</b></p><p><b>Conventional endoscopic mucosal resection as a cost-effective and potent option for diminutive rectal neuroendocrine tumors</b></p><p><b>Dong Jun Oh</b></p><p><i>Dongguk University Ilsan Hospital, Goyang, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Endoscopic mucosal resection (EMR) is being conducted for the treatment of small (≤10mm) rectal neuroendocrine tumors (NETs). Several studies suggest utilizing modified EMR to achieve complete resection. However, in resource-limited settings, modified EMR may be challenging to perform. And few studies have compared conventional EMR and modified EMR in the treatment of diminutive (≤5mm) rectal NETs. This study aims to compare the clinical outcome and cost-effectiveness of conventional EMR (cEMR) and cap assisted EMR (EMR-C) for diminutive rectal NETs.</p><p><b><i>Materials and Methods:</i></b> This single-center retrospective study included patients who underwent endoscopic treatment for diminutive rectal NETs between March 2022 and March 2024. All EMR procedures were performed by experienced endoscopists.</p><p><b><i>Results:</i></b> A total of 68 patients were enrolled. Seventeen patients underwent cEMR, while 48 patients underwent EMR-C. The mean histologic size of NETs was 3.5 ± 1.2 mm in the cEMR and 3.4 ± 1.4 mm in the EMR-C. The complete resection rate was 94.1% (16/17) in the cEMR and 93.8% (45/48) in the EMR-C, with no significant difference (p = 0.999). There were 3 cases of delayed bleeding in the EMR-C, while no complications were observed in the cEMR (p = 0.702). The mean hospitalization period for patients who underwent EMR-C was 2.1 days, while all EMR patients were managed on an outpatient setting.</p><p><b><i>Conclusion:</i></b> Based on the available endoscopic equipment and expertise, cEMR can be considered a completely resectable and cost-effective treatment option for diminutive rectal NETs. Further multicenter prospective studies are needed.</p><p><b>PP-01-086</b></p><p><b>Argon plasma coagulation versus endoscopic resection to treat gastric adenoma: A systematic review and meta-analysis</b></p><p><b>Jin Hwa Park</b><sup>1</sup>, Jae Gon Lee<sup>2</sup>, Sang Pyo Lee<sup>1</sup> and Kang Nyeong Lee<sup>1</sup></p><p><sup>1</sup><i>Hanyang University, Seoul, South Korea;</i> <sup>2</sup><i>Hallym University, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Endoscopic resection (ER) is regarded as the best treatment for gastric adenoma. Argon plasma coagulation (APC) is also widely used to treat gastric adenomas, but its efficacy and safety have not been fully established. We performed a systematic review and meta-analysis to determine the efficacy and safety of APC compared with ER in treating gastric adenoma.</p><p><b><i>Materials and Methods:</i></b> PubMed, EMBASE, and the Cochrane Library were searched up to April 2024. All studies that evaluated the clinical outcomes of APC or ER for treating gastric adenomas were included. Outcomes included local recurrence rate, procedure time, length of hospital stay, and complications.</p><p><b><i>Results:</i></b> A total of 7 studies were included, of which 3 were retrospective single-arm studies that reported only outcomes of APC, and 4 were retrospective studies that compared the outcomes of APC vs ER. APC showed a higher local recurrence rate in treating gastric adenoma than ER (risk ratio [RR] 4.378, 95% CI 1.995-9.607), but resulted in shorter procedure times (MD -45.228, 95% CI -49.436 to -41.021), shorter hospital stays (MD -2.684, 95% CI -2.932 to -2.437), and fewer complications (RR 0.329, 95% CI 0.124 to 0.869).</p><p><b><i>Conclusions:</i></b> APC was associated with a higher local recurrence rate, but a lower risk of complications than ER. APC can be considered an alternative to ER in treating gastric adenomas.</p><p><b>PP-01-087</b></p><p><b>Cold versus hot endoscopic mucosal resection of large non-pedunculated colorectal polyps: A meta-analysis</b></p><p><b>Federico Iv Peralta</b>, Nikko Theodore Raymundo and Enrik John Aguila</p><p><i>St. Luke's Medical Center - Global City, Taguig, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Large non-pedunculated colorectal polyps (>20mm) can be effectively managed with hot snare EMR (H-EMR); however, electrocautery-related complications may incur substantial morbidity. It has been suggested that cold snare EMR (C-EMR) may be better than H-EMR because of fewer adverse events. This meta-analysis aims to compare the outcomes of H-EMR and C-EMR.</p><p><b><i>Materials and Methods:</i></b> Relevant studies were identified through systematic searches of PubMed, MEDLINE, Cochrane, and Google Scholar databases. Out of 173, four eligible studies (2 randomized controlled trials and 2 retrospective studies) were included in the analysis. Quality assessment was performed using the Cochrane Risk of Bias tool for the RCTs and the Newcastle-Ottawa scale for the retrospective studies.</p><p><b><i>Results:</i></b> A total of 1293 large non-pedunculated colorectal polyps resected using EMR were included (367 C-EMR vs. 906 H-EMR). There is no statistically significant difference in the technical success rate of C-EMR compared to H-EMR (OR 2.11, 95% CI [0.14-31]; I²=67%). Additionally, there is no significant difference in the risk of perforation between the two methods (OR 0.23, 95% CI [0.04-1.30]; I²=0%). H-EMR has a higher risk of delayed bleeding compared to C-EMR (OR 0.2, 95% CI [0.07-0.56]; I²=0%). In contrast, C-EMR has been shown to have a higher rate of polyp recurrence compared to H-EMR (OR 2.56, 95% CI [1.40-4.70]; I²=43%).</p><p><b><i>Conclusion:</i></b> C-EMR is non-inferior to H-EMR in the resection of large nonpedunculated colonic polyp. C-EMR appears considerably safer with a lower risk of delayed bleeding than H-EMR; however, at the cost of a higher polyp recurrence rate.</p><p><b>PP-01-088</b></p><p><b>Pancreatic toxoplasmosis based on endoscopic ultrasound evaluation: A rare case report</b></p><p><b>Cosmas Rinaldi A Lesmana</b><sup>1,2</sup>, Randy Adiwinata<sup>1</sup> and Jennifer Simsca<sup>1</sup></p><p><sup>1</sup><i>Gastrointestinal Cancer Center, MRCCC Siloam Hospital Semanggi, Jakarta, Indonesia;</i> <sup>2</sup><i>Division of Hepatobiliary, Department of Internal Medicine, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine Universitas Indonesia, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Toxoplasmosis can present as systemic disease affecting many organs, especially in immunocompromised patients. Most cases of toxoplasmosis present as encephalitis, while extracerebral toxoplasmosis is rare, particularly in gastrointestinal tract. Here we reported 48-year-old HIV infected male patients with toxoplasmosis manifesting as pancreatic nodule, chronic pancreatitis and encephalitis</p><p><b><i>Case description:</i></b> A 48-year-old male HIV patient was referred to our hospital for evaluation of pancreatic nodules. He had been hospitalized in previous hospital due to hemiparesis and dizziness. Brain MRI with contrast revealed solid-occupying-lesion in cerebellum with surrounding edema. PET scan indicated focal metabolic sign in the head and body of pancreas suggesting for primary pancreatic tumor. Lipase was elevated below 2 times of upper-limit-normal. Amylase, Ca 19-9, and CEA was normal. We performed endoscopic ultrasound (EUS) and we found multiple hypoechoic nodules in pancreatic head and body. EUS fine needle biopsy (FNB) using 22-G acquire needle (Boston Scientific, USA) was then performed for further evaluation. Histopathology examination showed chronic pancreatitis, no malignant cell, and bradyzoite-containing tissue cysts suggestive of toxoplasmosis. He was treated for toxoplasmosis by infectious disease specialist.</p><p><b><i>Discussion:</i></b> Pancreatic Toxoplasmosis is rarely reported due to its uncommon occurrence, even in HIV patients. Ahuja et al. reported a case of necrotizing pancreatitis and multisystem organ failure associated with toxoplasmosis in AIDS patient. Toxoplasma may directly invade pancreatic organ and induce inflammation. Differential diagnosis for hypoechoic pancreatic lesion includes chronic pancreatitis, neuroendocrine tumor, lymphoma, or metastatic tumor. EUS FNB has emerged as valuable diagnostic procedure for evaluating pancreatic lesion.</p><p><b>PP-01-089</b></p><p><b>Diagnostic yields and clinical impact of endoscopic ultrasound-guided tissue acquisition for biliary tract lesions</b></p><p><b>Daiki Agarie</b>, Susumu Hijioka and Takuji Okusaka</p><p><i>National Cancer Center Japan, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed at clarifying the efficacy and safety of endoscopic ultrasound-guided tissue acquisition (EUS-TA) for diagnosing biliary tract lesions.</p><p><b><i>Materials and Methods:</i></b> Puncture targets included patients with biliary tract lesions. Cases in which lymph nodes or liver metastases were punctured were excluded. Diagnostic yields of EUS-TA and adverse event rates were evaluated for each lesion site. Information on cases in which Endoscopic retrograde cholangiography (ERC) guided biopsy/cytology was performed was also collected.</p><p><b><i>Results:</i></b> EUS-TA, attempted in 56 patients with biliary tract lesions, 45 were malignancy and 11 were benign respectively, had a sensitivity, specificity, and diagnostic accuracy of 95.6%, 100%, and 96.4% (54/56), respectively. The diagnostic accuracy for hilar bile duct lesions (90.5%; 20/22) was lower than that for the other sites (100%; 34/34; p=0.15). Procedure-related adverse events were observed in one case (1.8%) of mild bile leakage peritonitis with punctured hilar bile duct lesion.</p><p>Moreover, 217 cases of ERC guided biopsy/cytology were performed on suspected malignant biliary tract lesions in the study period, with diagnostic accuracy of 82.0% (178/217); the addition of endoscopic ultrasound-guided tissue acquisition for suspected false-negative cases resulted in an elevated diagnostic accuracy (93.1%; 202/217; p<0.001).</p><p><b><i>Conclusion:</i></b> EUS-TA is an accurate and safe test for the diagnosis of biliary lesions. And it is also effective for the cases that are difficult to diagnose by ERC, thus the addition of EUS-TA should be considered when false negative ERC results are suspected.</p><p><b>PP-01-090</b></p><p><b>Efficacy EUS-BD in the treatment of malignant distal biliary and gastric/duodenal outlet obstruction</b></p><p><b>Catarina Budyono</b> and Rabbinu Rangga Pribadi</p><p><i>Fellow of Gastroenterohepatology Department of Internal Medicine Faculty of Medicine Universitas Indonesia / Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia;</i> <i>Division of Gastroenterology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Endoscopic ultrasonography-guided biliary drainage (EUS-BD) is one of the alternative methods in treating malignant biliary obstruction (MBO) when endoscopic retrograde cholangiopancreatography (ERCP) cannot be performed due to inaccessibility. This method is classified into three techniques, i.e. EUS-guided choledocoduodenostomy (EUS-CDS), EUS-guided hepatogastrostomy (EUS-HGS) and EUS-guided antegrade stenting (EUS-AGS).</p><p><b><i>Case Report:</i></b> A 44-year-old female was consulted from digestive surgeon with chief complaints of jaundice since a month ago and fever since 1 week ago. Physical examination showed icteric sclera and skin. Laboratory examination showed increased leukocytes, decreased hemoglobin and hematocrit. In addition, total bilirubin 14.92 mg/dL, direct bilirubin 10.5 mg/dL, and indirect bilirubin 4.42 mg/dL. MRI-MRCP examination showed solid mass at pancreatic head and body, with CBD stricture and upstream dilatation of CHD and bilateral intrahepatic bile duct. When ERCP was performed, there was stenosis and duodenal bulb ulcer, so the coverage could not reach D-2. Five days post EUS-AGS and EUS-HGS, there was a decrease in total, direct, and indirect bilirubin levels to 4.8 mg/dL, 3.41 mg/dL, and 1.39 mg/dL respectively. In addition, acute cholangitis resolved and the patient underwent biliodigestive bypass.</p><p><b><i>Discussion:</i></b> EUS-BD is expected to be the main alternative in managing biliary drainage. When compared to percutaneus transhepatic biliary drainage (PTBD), which is also an alternative to ERCP, EUS-BD has a higher success rate and lower complication rate. EUS-BD also provides better comfort and nutrient absorption, and avoids electrolyte loss. The technical and clinical success of EUS-BD is higher than ERCP in pancreatic patients with indwelling gastro-duodenal stents.</p><p><b>PP-01-091</b></p><p><b>Paradigm shift: Case report of synergistic use of eus-guided gastrojejunostomy(EUS-GJ) and Hepaticogastrostomy(EUS-HGS) for malignant obstruction</b></p><p><b>Wah Loong Chan</b>, Farahani Khamis, Khairiah Ahmad and Stanley Khoo</p><p><i>University Of Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To report the successful use of endoscopic ultrasound-guided gastrojejunostomy (EUS-GJ) and hepaticogastrostomy (EUS-HGS) in managing gastric outlet and biliary obstructions in a patient with metastatic pancreatic adenocarcinoma.</p><p><b><i>Case Description:</i></b> A 47-year-old female with metastatic neck of pancreas adenocarcinoma, previously treated with FOLFIRINOX, presented with post-prandial vomiting, abdominal pain, and lethargy. Clinical examination revealed jaundice and epigastric tenderness. Past interventions included duodenal stenting and EUS-guided biopsy confirming adenocarcinoma.</p><p><b><i>Results:</i></b> Laboratory tests showed worsening liver function with hyperbilirubinemia (126 μmol/L), Alanine Transaminase (ALT) 449 U/L, Aspartate Aminotransferase (AST) 214 U/L, Gamma-glutamyl Transferase (GGT) 721 U/L, and Alkaline Phosphatase (ALP) 363 U/L. CA 19-9 had increased to 5882 U/mL. Gastroscopy revealed a displaced and disintegrated duodenal stent with tumoral invasion. Positron emission tomography–computed tomography (PET-CT) demonstrated disease progression with worsening metastases and biliary obstruction. EUS-GJ was performed using a 20mm x 10mm electrocauterylumen-apposing metal stent (LAMS). EUS-HGS was completed using a 10cm partially covered hybrid self-expandable metal stent (SEMS). Post-procedure, the patient’s liver function improved and she resumed a soft diet.</p><p><b><i>Discussion and Conclusion:</i></b> This case demonstrates the success of combined EUS-GJ and EUS-HGS in managing complex malignant obstructions. Some studies demonstrated that EUS-GJ to be superior to enteral stenting, with lower reintervention rates and higher clinical success rates. EUS-HGS has emerged as a viable alternative to percutaneous drainage. The positive outcome in this case highlight the importance of learning these advanced techniques from local and international experts to elevate the standard of care in managing patients with advanced malignancies.</p><p><b>PP-01-092</b></p><p><b>Comparing safety, clinical success, and re-intervention in EUS-gastroenterostomy vs. Enteral metal stents in malignant GOO</b></p><p>Arun Arora Pagadapelli and <b>Amit Daphale</b> and Rohan Yewale and Amol Bapaye</p><p><i>Deenanath Mangeshkar Hospital, Pune, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>This study aimed to compare the safety, clinical outcomes, and rates of re-intervention between two palliative treatments for malignant gastric outlet obstruction(GOO): EUS-guided gastroenterostomy(EUS-GE) and enteral self-expanding metal stents(SEMS). Over 3.5 years(December 2019–August 2023), a retrospective analysis was conducted on 50 patients(20 EUS-GE, 30 enteral SEMS). Both groups, had mean ages around 62 years, predominantly male. Technical success rates were 90% for EUS-GE and 100% for enteral SEMS, with comparable clinical success rates. Mean hospital stays were 4.8 days ± 2.9 days for EUS-GE and 3.5 days ± 3.0 days for enteral SEMS (p=0.133). Most common cause for GOO in both groups was Carcinoma head of pancreas. Location of GOO was Proximal duodenum in 75% of patients in EUS-GE group and 66.7% in Enteral SEMS group(p=0.529), antrum in 25% of patients in EUS-GE group and 33.7% in Enteral SEMS group. Re-intervention was not required in the EUS-GE group, while two patients in the enteral SEMS group needed it(p=0.16). Median survival was similar in both groups, approximately 5.4 ± 4.6 months for EUS-GE and -5.5 ± 4.2 months for enteral SEMS(p=0.976).</p><p>Study concluded that both EUS-GE and enteral SEMS showed comparable efficacy in terms of technical success, clinical outcomes, safety profiles, and re-intervention rates. However, the limitation of a median survival of less than 6 months for both groups might have influenced outcomes. For patients with expected survival less than 6 months, enteral SEMS appeared non-inferior. Future studies focusing on long-term survival are necessary to determine if one method is superior.</p><p><b>PP-01-093</b></p><p><b>EUS guided Hepatic Solid mass (HSM) biopsy: The New Paradigm?</b></p><p><b>Pritam Das</b> and Samir Mohindra and Dhruv Thakur and Gourav Borah and Naganath K Wodeyar and S Rakesh Kumar</p><p><i>KGMU, Lucknow, India, Lucknow, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The advent of EUS guided procedures expanded the horizon of diagnostic and therapeutic procedures with real-time imaging and greater sensitivity of detection of smaller lesions (< 1 cm). EUS guided Hepatic Solid mass (HSM) biopsy had advantages over the traditional method (Percutaneous Ultrasound Guided) with ability to improved detection rate of smaller lesions (< 1 cm), diagnosis of nature of the lesion as well as in accordance to surrounding structures, performing therapeutic procedures (e.g., biliary drainage).</p><p><b><i>Method:</i></b> This was a hospital based, observational prospective pilot study conducted in a tertiary centre in a period of one year. We included all patients of age ≥ 18 years old referred for liver biopsy for HSM. The aim of the study was assessment of the diagnostic yield and safety of Endoscopic Ultrasound Guided liver biopsy (FNB) in HSM. The primary outcome Measures included diagnostic adequacy of the tissue sample.</p><p><b><i>Results:</i></b> The study included a total of 34 patients, of which FNB was done in 32 patients. Diagnostic Adequacy was present in 29/32 (90.6%) patients, inability to diagnose 3/32 (9.4%). The total number of core samples taken were 3.46 ± 1.29. The mean size of longest intact core was 6.625 ± 4.399 mm. The total sample length was 12.531 ± 6.968 mm.</p><p><b><i>Conclusion:</i></b> Although the use of 16G needle by percutaneous method increases the diagnostic accuracy of the pathology specimen, EUS guided method (19G, 22G) yielded good diagnostic yield with minimal complication rates inspite of relatively smaller longest intact core sample.</p><p><b>PP-01-094</b></p><p><b>Evaluation of cryoablation using a prototype cryoablation needle in swine liver</b></p><p>Jonghyun Lee<sup>1,2</sup>, Hyunjoon Son<sup>3</sup>, Tae In Kim<sup>1,2</sup>, Dong Uk Kim<sup>4</sup>, Daejin Kim<sup>3</sup>, Gun-Ho Kim<sup>3</sup> and <b>Sung Yong Han</b><sup>1,2</sup></p><p><sup>1</sup><i>Division of Gastroenterology, Department of Internal Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, South Korea;</i> <sup>2</sup><i>Department of Internal Medicine, School of Medicine, Pusan National University, Busan, South Korea;</i> <sup>3</sup><i>Department of Mechanical Engineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea;</i> <sup>4</sup><i>Department of Internal Medicine, CHA Gumi Medical Center, CHA University, Gumi -si, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> Pancreatic cancer presents a significant challenge due to its late-stage diagnosis and high mortality rate. Cryoablation, a method employed for treating several cancer types, has shown promise in combination with other therapies for improving the prognosis of pancreatic cancer; however, its application is hindered by limitations such as lengthy procedures and specialized equipment. This study focused on developing a cryoablation needle suitable for endoscopic ultrasonography that could facilitate easier application in pancreatic cancer treatment.</p><p><b><i>Methods:</i></b> This study involved cryoablation experiments on swine liver tissue using cryo-needles to assess the extent of cell death at various cryoablation temperatures and durations.</p><p><b><i>Results:</i></b> The cryoablation system utilized liquid carbon dioxide and achieved rapid cooling, reaching temperatures below -60°C within 30 s and sustaining cryoablation for 200 s. These conditions induced liver tissue necrosis. Significant cell changes were observed up to 15 mm from the cryoablation needle.</p><p><b><i>Conclusions:</i></b> This experimental study demonstrated the effectiveness of cryoablation with a cryo-needle in the swine liver. Further trials on pancreatic tissue are anticipated to confirm its effectiveness, with ongoing research crucial to establishing its value as an adjunct therapy.</p><p><b>PP-01-095</b></p><p><b>EUS provocation test to identify the origin of unexplained upper abdominal pain</b></p><p><b>Hyun Don Joo</b></p><p><i>Hanyang University, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Identifying the source of abdominal discomfort can be challenging, necessitating direct stimulation of the affected organ. However, achieving accurate stimulation is sometimes difficult. This study proposes a new method using endoscopic ultrasound (EUS) to pinpoint the origin of unclear upper abdominal pain.</p><p><b><i>Materials and Methods:</i></b> Patients with unexplained upper abdominal pain, despite undergoing extensive medical evaluations, were enrolled. They underwent an EUS provocation test (EPT), which involved endoscopic inspection and mechanical stimulation such as stretching, torsion, and compression. A positive EPT result was determined if the patient experienced pain similar to their usual discomfort.</p><p><b><i>Results:</i></b> From January 2015 to December 2020, EPT identified the origin of pain in 15 out of 17 patients (88.2%). Gastric stimulation yielded positive results in nine patients, cholecystic stimulation in three, and pancreatic stimulation in three. Two patients experienced pain that was not localized and resembled their usual discomfort.</p><p><b><i>Conclusion:</i></b> This study demonstrates that EPT, a novel application of EUS, has a high sensitivity for identifying the origin of ambiguous abdominal pain. Targeting the organs identified through EPT could enhance treatment effectiveness.</p><p><b>PP-01-096</b></p><p><b>Outcome of ultrasound endoscopic gallbladder drainage for acute cholecystitis at single institution</b></p><p><b>Fumio Kakizaki</b></p><p><i>Cancer and Infectious Disease Center Komagome Hospital, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> EUS-guided gallbladder drainage (EUS-GBD) has been reported in patients with poor surgical tolerance. However, indications, techniques, equipment, and efficacy of EUS-GBD procedure are inconsistent and are equivocal in Japan. Therefore, this study aimed to evaluate the results and safety of EUS-GBD cases.</p><p><b><i>Materials and Methods:</i></b> This study retrospectively evaluated the clinical characteristics of 10 patients who underwent EUS-GBD at Komagome Hospital between July 2018 and May 2024.</p><p><b><i>Results:</i></b> (Patient Background) Median age was 84.5 years, male/female: 4/6, ASA-PS 0/1/2/3: 3/3/2/2, mean hospital stay was 30.4 days, and background diseases were dementia/degraded ADL/terminated (including benign and malignant)/other (failure of other treatments, comorbidities): 3/1/3/3. (Procedure details)Approach site: duodenal bulb/gastric: 9/1, stent type: plastic stent /ENBD: 9/1, fistula dilation rate: 90% (9/10), fistula dilation type: electric current/electric current + mechanical/mechanical: 7/1/1, procedure time: insertion to puncture/puncture to stent placement: 10 min /14.5 min. The procedural success rate was 90%, and the reason for failure was stent migration. The clinical success rate was 89% (8/9), and the reason for failure was prolonged fever. The complication rate was 20% (2/10), including two complications (perforation and stent migration) observed in the early induction stage of the EUS-GBD procedure, but have been treated without complications since then.</p><p><b><i>Conclusion:</i></b> The EUS-GBD procedure is a relatively safe and effective EUS technique for patients with acute cholecystitis in the late stages of dementia or cancer.</p><p><b>PP-01-097</b></p><p><b>Atypical endosonographic features of pancreatic neuroendocrine tumors</b></p><p><b>Valeriia Kamalova</b>, Evgeniy Solonitsyn, Dmitriy Baranov and Evgeniy Lebedev</p><p><i>Almazov Nmrc, Saint Petersburg, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> It is generally accepted that pancreatic neuroendocrine tumors (PNETs) on endosonography always appear as solid hypoechoic tumors, often multiple, with clear smooth edges, and hypervascular on Dopler scan. As experience with such patients accumulated, we noted that the appearance of PNETs can be highly variable; this study is dedicated to the descriptions of these cases.</p><p><b><i>Materials and Methods:</i></b> This is a retrospective single-center study conducted from 2020 to 2024. Patients with PNETs verified by EUS-FNA (Endoscopic Ultrasound-Guided Fine Needle Aspiration) were included in the study. The study included 55 patients.</p><p><b><i>Results:</i></b> Among the 55 patients, the male-to-female ratio was 1:1.66, with an average age of 62 (range 18-81). Neuroendocrine tumors were located with equal frequency in the head, body, and tail of the pancreas; in 23.2% of cases, multiple lesions were noted. The average size of the tumors was 30 mm (range 5-80 mm). Among the 55 tumors, 7.2% appeared as cystic-solid lesions, and 5.4% as cystic. In some cases, the PNETs had an unclear irregular border (5.4%), and a small portion were avascular on Doppler imaging (3.6%). All patients underwent EUS-FNA for diagnostic verification, and in 19.8% of cases, the presumed diagnosis based on the EUS image was not confirmed.</p><p><b><i>Conclusion:</i></b> When performing EUS, it is important to remember the characteristic appearance of tumors; in some cases, to establish the correct diagnosis, it is necessary to correlate clinical data, visual characteristics, and perform EUS-FNA.</p><p><b>PP-01-098</b></p><p><b>Endoscopic ultrasound liver biopsy (EUS-LB) vs trans jugular liver biopsy (TJ-LB), in a special population</b></p><p><b>Shivam Khare</b> and Anil Arora</p><p><i>Sir Ganga Ram Hospital, Delhi, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Gold standard route for liver biopsy is percutaneous-liver-biopsy(PC-LB) in absence of coagulopathy, thrombocytopenia and ascites.Trans-jugular-liver-biopsy(TJ-LB) is consider main route,in presence of coagulopathy,thrombocytopenia and ascites.Now,there is enough data available which showed non-inferior efficacy and safety of EUS-guided-liver-biopsy(EUS-LB) in comparison to PC-LB.EUS-LB offers certain advantages over traditional methods such as,one can obtain good sample in presence of ascites by getting ascites free window and similarly,in early coagulopathy by choosing vessel free approach on doppler.TJ-LB is a cumbersome procedure,therefore, Aim of the study is to compare the EUS-LB and TJ-LB in terms of adequacy and safety,in a special population where PC-LB is contraindicated but EUS-LB can perform(ascites/mild coagulopathy{INR between 1.5-2}) instead of TJ-LB.</p><p><b><i>Methods and material:</i></b> This is a retrospective study. Adequacy of tissue diagnosis and Adverse events were compared between the two groups.</p><p><b><i>Results:</i></b> 50 patients were enrolled in TJ-LB group, 40 in EUS-LB. Both groups were propensity matched in terms of age,gender,ascites,INR and child status. Total specimen length(TSL) and number of complete portal tract(CPT) were more in EUS-LB group than in TJ-LB group(3.5±0.9cm vs 1.7±1cm with p-value 0.000 and 19±11 vs 10.6±5.7 with p-value 0.000, respectively).Fragmentation was higher in EUS-LB vs TJ-LB(4.5±2.3 vs 2.7±1.3, p<0.05),However, longest fragment length were significantly higher in EUS-LB vs TJ-LB(mean 1.9cm vs 0.7cm).Adequate biopsy rate was 100% in EUS-LB and 96% in TJ-LB.No major complications occurred in the both group.</p><p><b><i>Conclusion:</i></b> This first unique study from Asia is comparing EUS-LB and TJ-LB in special population.TSL,CPT,Adequate biopsy rate,Conclusive diagnosis and longest fragment length were more in EUS-LB than TJ-LB group.</p><p><b>PP-01-099</b></p><p><b>EUS-guided cystgastrostomy in pancreatic pseudocyst using electrocautery-enhanced lumen apposing metal stent delivery system</b></p><p><b>Hannah Angelica Lacar</b>, Bin Chin, Josef Carlo Lazaro and Angela Djajakusuma</p><p><i>The Medical City, Pasig, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pancreatic pseudocyst is an encapsulated collection of homogeneous fluid with little or no necrotic tissue located near the pancreas in patients with a history of pancreatitis. It occurs when the damaged pancreatic ducts cause extravasation and collection of the pancreatic fluid.</p><p><b><i>Case Description:</i></b> The patient is a 44-year-old-female, diabetic, who experienced a 2-week period of intermittent crampy epigastric pain radiating to the back which progressed to persistent severe boring pain. On contrast CT scan, she had acute pancreatitis with peripancreatic fluid collection and was discharged. In the interim, she had abdominal fullness and on follow-up CT scan showed an increase in size of the well-defined fluid collections anterior to the pancreas, previously ascribed to a pseudocyst or walled off necrosis. A dilated pancreatic duct with abrupt cut off at the region of the pancreatic head. On EUS, there was a large well-encapsulated fluid collection posterior to the stomach measuring 15cm x 11cm. EUS-guided cystgastrostomy using Electrocautery-enhanced Lumen Apposing Metal Stent Delivery System was done and was able to drain 2 liters of pancreatic fluid. After 4 weeks, abdominal MRI MRCP showed no pancreatic ductal obstruction and on repeat EUS, there was a significant decrease in the pancreatic fluid collection. The stent was then successfully removed.</p><p><b>PP-01-100</b></p><p><b>Recurrent hematemesis unveiling gastric tuberculosis: A case report emphasizing the diagnostic value of endoscopic ultrasound</b></p><p><b>Ronell Lee</b>, Mary Anne Go and Michael Louie Lim</p><p><i>Chinese General Hospital And Medical Center, Manila, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>In 2021, the Philippines ranked fourth globally in tuberculosis cases, with 650 individuals affected per 100,000, designating it as a high TB burden country. Gastric tuberculosis, though rare, poses diagnostic challenges due to clinical manifestations resembling more prevalent gastric pathologies. Gastrointestinal bleeding, typically associated with peptic ulcers, esophageal varices, or gastric malignancies, can occasionally indicate infectious processes like tuberculosis, especially in high-prevalence regions.</p><p>This report details the diagnostic journey of a 35-year-old male with recurrent hematemesis. Initial investigations, including upper gastrointestinal (GI) endoscopy and CT scans, failed to yield a definitive diagnosis. Subsequent GI endoscopy revealed a 2.0cm mucosal protrusion with a clean-based ulcer, emphasizing the importance of advanced diagnostic techniques like endoscopic ultrasound (EUS). EUS identified a hypoechoic lesion at the muscularis mucosa, with multiple hypoechoic lymph nodes. The fine-needle biopsy during EUS confirmed Mycobacterium tuberculosis (MTB) infection.</p><p><b>PP-01-101</b></p><p><b>Lower esophageal sphincter of achalasia patients under endoscopic ultrosongraphy : genesis and therapeutic outcomes</b></p><p><b>Cong Liu</b>, Lili Zhang, Wei Zhao, Bin Whang, Hong Jin, Shujin Li, Shiwei Ru and Xuechai Liu</p><p><i>Tianjin Medical University General Hospital, Tianjin, CN, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the thickness and histological properties of lower esophageal sphincter (LES) in achalasia (AC) patients as well as its relationship with the prognosis of per-oral endoscopic myotomy (POEM).</p><p><b><i>Materials and Methods:</i></b> Fifteen AC patients preparing for POEM as preliminary therapy and sixteen patients with submucosal tumor of stomach who accepted EUS as control group I were enrolled. Thickness of LES was measured and compared between groups. Ten patients who underwent distal esophagectomy for gastric cancer as control group II were also included, and the similar muscle was taken for histological evaluation. According to thickness of LES, AC patients were subdivided into thicker and thinner subgroups. The pathological features were assessed by H&E staining and compared between groups. In addition, the prognosis of AC were also compared between groups.</p><p><b><i>Results:</i></b> The LES, internal circular muscle and outer longitudinal muscle of AC patients were thicker than that of control group I, respectively. AC patients showed severer atrophy and fibrosis than control group II. Furthermore, AC patients were subdivided to thicker and thinner LES group (with 3mm as standard), severer fibrosis was found in thicker group. There was no significant difference in response rate, Eckardt score and reflux rate between the thinner and thicker LES groups, while the Demesster score 2 years after POEM was higher in thicker group.</p><p><b><i>Conclusion:</i></b> There was thicker LES of AC patients, which was associated with atrophy and fibrosis. AC patients with thicker LES under EUS was predictive of a severer GER two years after POEM.</p><p><b>PP-01-102</b></p><p><b>Comparison of EUS PV versus intra-operative direct pressure measurements in pre-transplant cirrhotic- pilot study</b></p><p><b>Singh Aniruddha Pratap Haripal Lnu</b>, SHUJAATH ASIF, Azimudin Haja, PRADEV INAVOLU, HARDIK RUGHWANI, Mithun Sharma, Sana Fathima Memon, GV Prem Kumar, P Kumaraswamy, Balachandra Menon, Duvvur Nageshwar Reddy and SUNDEEP LAKHTAKIA</p><p><i>Aig Hospitals, Hyderabad, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Direct portal pressure measurement under EUS-guidance (EUS-PP) measurement is a promising alternative to conventional indirect Hepatic Venous Pressure Gradient (HVPG). In patients undergoing liver transplantation for cirrhosis, high pre-operative portal vein (PV) pressure requires intra-operative portal vein inflow modulation (PIM). This pilot study aims to assess correlation between EUS-PP with direct intra-operative portal pressure measurement (IO-PP).</p><p><b><i>Methods:</i></b> Cirrhosis patients scheduled for liver transplant within next 48 hours were included. EUS-PPM was performed using a 22G EUS-FNA needle to puncture PV. Both, compact manometer (CM) and arterial pressure transducer (PT) were used to measure the intra-vascular pressure, pre- and intra-operatively. All adverse events were recorded. IO-PP was done just soon after laparotomy using PT.</p><p><b><i>Results:</i></b> 25 patients, 23/25 were males. 19, 5 & 1 patients were CHILD PUGH C, B and A respectively.</p><p>Procedural technical success was 100 %. EUS guided pre-transplant PP using novel CM & PT was 27.12 +/- 6.2 mmHg and 31.68 +/- 10.2 mmHg, respectively. Intraoperative measurements just after laparotomy using CM & PT were 22.76 +/- 6.09 mmHg & 22.4 +/- 5.10 mmHg, respectively. 24-hour post liver transplant portal pressure measurements using CM & AT were 9.44 +/- 1.70 mmHg & 8.8 +/- 1.77 mmHg, respectively. Only 3/25 patients had mild hematomas and did not require any additional surgical intervention.</p><p><b><i>Conclusion:</i></b> EUS guided direct PV puncture & PP measurement using compact manometer is a safe and correlates well with PT, both preoperatively & intra-operatively. Multi-centric validation with higher numbers study are needed.</p><p><b>PP-01-103</b></p><p><b>Endoscopic Ultrasound(EUS)-Guided Biopsy; A Diagnostic Tool in Lymphadenopathy Surrounding Gastrointestinal Tract: A tertiary center experience</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Githma Wimalasena<sup>1</sup>, Mithushan Jesuthasan<sup>1</sup>, Duminda Subasinghe<sup>1,2</sup>, Sivasooriya Sivaganesh<sup>1,2</sup>, Harshima Wijesinghe<sup>3</sup>, Amarathunga Priyani<sup>3</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital, Sri Lanka, Colombo;</i> <sup>3</sup><i>Department of Pathology, Faculty of Medicine, University of Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> EUS with transluminal biopsy has revolutionized the diagnosis of patients with enlarged lymph nodes (LN) of the mediastinum, para-aortic and hilar regions where traditional more invasive surgical approaches were the gold standard. This pioneering study in Sri Lanka highlights the effective use of EUS in obtaining histology of LNs in these sites with difficult access.</p><p><b><i>Materials and Methods:</i></b> From March 2018 to March 2024, 27 patients with LN masses requiring EUS-guided biopsy were included in this study. A single endoscopist (ANR) performed the procedures.</p><p><b><i>Results:</i></b> The mean age was 49.44 years (SD 15.52) with 16 (59.3%) male patients. LN locations were as follows: 11 (40.7%) para-aortic, 5 (18.5%) porta hepatis, 4 (14.8%) mediastinal, 4 (14.8%) celiac, and 3 (11.1%) para-oesophageal. The LN sizes ranged from 10 to 47 mm. Diagnoses were available in 21 (77.8%) cases, in 6 the diagnosis remained inconclusive. Among the positive diagnoses, 11 (52.4%) were malignant, 7 (33.3%) benign, and 3 (14.28%) infective. The diagnoses included 4 (18.5%) Adenocarcinoma, 3 (11.1%) Hodgkin lymphoma, 2 (7.4%) non-Hodgkin lymphoma, 2 (7.4%) poorly differentiated carcinoma, and 3 (11.1%) tuberculosis cases. No complications occurred during the procedure.</p><p><b><i>Conclusion:</i></b> EUS-guided biopsy is a safe and effective diagnostic tool for LN masses accessible via the upper gastrointestinal tract, providing a non-invasive alternative to surgical methods.</p><p><b>PP-01-104</b></p><p><b>Endoscopic Ultrasound(EUS) guided biopsy as a diagnostic tool in upper-gastrointestinal(UGI) lesions, A tertiary center experience</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Githma Wimalasena<sup>1</sup>, Mithushan Jesuthasan<sup>1</sup>, Duminda Subasinghe<sup>1,2</sup>, Sivasooriya Siwaganesh<sup>1,2</sup>, Harshima Wijesinghe<sup>3</sup>, Amarathunga Priyani<sup>3</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital, Colombo, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, Faculty of Medicine, University of Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Despite its widespread use internationally, EUS-guided transluminal biopsy of UGI lesions is rarely requested and therefore no data is available in Sri Lanka. Without histology, these patients may have had to undergo surgery or follow-up with prolonged anxious periods. This study outlines the experience of a tertiary care referral center.</p><p><b><i>Materials and Methods:</i></b> From March 2018 to March 2024, EUS-guided biopsy was performed on 46 patients with UGI tract lesions by a single endoscopist (ANR).</p><p><b><i>Results:</i></b> The mean age was 57.93 years (SD=16.375) with 30 (65.2%) males. Lesion sites were, stomach (n=17,37.0%), gastro esophageal junction (GOJ) (n=10, 21.7%), duodenum (n=10, 21.7%) and esophagus (n=9, 19.6%). The majority were solid (n=42, 91.35%), sizes ranging from 12 -34mm. No complications were seen.</p><p>A diagnosis was made in 91.3% (n=42) cases with only 2 (4.3%) inconclusive and 2 (4.3%) inadequate tissue. 16 (34.8%) were malignant. Following are location specific diagnoses. Esophageal: Gastrointestinal Stromal Tumors (GIST) (n=2), spindle cell tumour (n=2) and normal tissue (n=2). GOJ: Leiomyoma (n=4), squamous cell carcinoma (n=2), GIST/leiomyoma (n=2), normal tissue (n=1). Stomach: GIST (n=6), poorly cohesive carcinoma (n=1), GIST/leiomyoma (n=2), spindle cell tumour (n=2), gastric adenoma (n=2). Duodenum: Adenocarcinoma (n=3), GIST (n=2), Neuro Endocrine Tumor (n=2), Poorly cohesive carcinoma (n=2).</p><p><b><i>Conclusion:</i></b> EUS guided biopsy is an effective and safe diagnostic tool for UGI lesions which can be utilized in Sri Lanka to provide histological diagnosis and determine appropriate treatment.</p><p><b>PP-01-105</b></p><p><b>EUS-guided coil in the management of gastric varices- A single center case series in Malaysia</b></p><p><b>Ram Prasad Sinnanaidu</b> and Khoo Stanley</p><p><i>University Of Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Aims:</i></b> Gastric varices (GV) have high rates of rebleeding and mortality. Current endoscopic therapies are premised on endoscopic cyanoacrylate injection, a non-targeted therapy and carries a higher risk of rebleeding. Endoscopic Ultrasound (EUS) guided therapy in lately has gain much attraction in the management of GV. We present a case series of EUS guided coiling in combination with cyanoacrylate (CYA) injection for the treatment of GV.</p><p><b><i>Methods:</i></b> Six patients with GV underwent EUS guided coiling and CYA injection from July 2021 to December 2023. Embolization coils (size 12mm to 16mm) were inserted under EUS guidance followed by CYA injection. Obliteration of GV was confirmed by doppler imaging and endoscopic visualization. All patients were given prophylactic antibiotics. The baseline characteristics, technical success, clinical success, rebleeding rates and adverse events were recorded.</p><p><b><i>Results:</i></b> Among the 6 patients included, the most common etiology of GV was cirrhosis (83%), with MAFLD being the most common cause (80%). The main indication for therapy was primary prophylaxis (50%). 67% had GOV-2 with mean GV size of 18±6.0mm and the mean number coils used were 2±1.1. All patients achieved technical and clinical success. There were no intraprocedural adverse events, however 3 patients developed low grade fever post procedure requiring antibiotics and hospital admission. Only one patient had delayed rebleeding after 6 weeks.</p><p><b><i>Conclusions:</i></b> Combination of EUS coil and CYA therapy is an effective management for gastric varices with acceptable safety profile. Transient fever seen in half of the patients, was an interesting observation which needs further exploration.</p><p><b>PP-01-106</b></p><p><b>Replacement technique for dislocated lumen-apposing metal stent in endoscopic necrosectomy by the forceps</b></p><p><b>氏 Hiroshi Takihara</b></p><p><i>Uji Tokushukai Hospital/ Gastroenterology, kyoto, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Lumen-Apposing Metal Stents(LAMS) are essential for endoscopic necrosectomy(EN) in severe pancreatitis. However, LAMS often drops out during EN. We present a simple method to replace a dislocated LAMS by a forseps.</p><p><b><i>Case Description:</i></b> A 52-year-old man suffered from severe pancreatitis caused by gallstones. He was treated with endoscopic sphincterotomy(EST) and recovered well. But 1 week later,He developed septic shock and abdominal compertment syndrom with massive ascites. We urgently performed percutaneous drainage and placed LAMS(Hot-AxiosTMstent20mm) in posterior wall of gastric antrum.</p><p>3weeks later,we performed EN in the walled-off necrosis(WON). There was massive necrotic tissue and we reperted EN 3times.</p><p>During necrosectomy, LAMS interfered with snare operations. then we removed the LAMS through the esophageal overtube and after EN, we replaced the LAMS by a forseps.</p><p>8th days later necrosectomy, there were delayed bleeding of pseudoaneurysm. So we plosedured percutaneous vascular embolization. After that, He recovered well at all.</p><p><b><i>Discussion:</i></b> It is a simple method to replace a dislocated LAMS by the forseps. This case had a large cavity, it was easy to replace the LAMS. If the cavity is not enough to replace it, we have to plosedure another method like “through the channel replacement”.</p><p><b>PP-01-107</b></p><p><b>The diagnostic performance of endoscopic ultrasound guided fine-needle biopsy of solid pancreatic lesion</b></p><p><b>Nicholas Tee</b>, Weicong Lin, Weiquan Li, Andrew Boon Eu Kwek and Tiing Leong Ang</p><p><i>Changi General Hospital, Singapore, Singapore</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Endoscopic ultrasound (EUS) - guided tissue acquisition is important to establish a histological diagnosis of solid pancreatic lesions. The objective of this study is to evaluate the diagnostic performance of EUS-guided fine needle biopsy (FNB) of solid pancreatic lesions performed in our endoscopy center compared to the recommended societal standards.</p><p><b><i>Materials and Methods:</i></b> Data from patients who underwent EUS-guided FNB for solid pancreatic lesions, were retrospectively retrieved from January 2020 to December 2023. The overall sensitivity, specificity, accuracy of diagnosis and sample adequacy were analyzed. The diagnostic performance between 20G, 22G and 25G FNB needles were also analyzed. The FNB needles primarily used in our center were EchoTip ProCore needles, Cook Medical. All EUS procedures were performed by credentialed endosonographers.</p><p><b><i>Results:</i></b> A total of 167 patients were analyzed. The overall tissue acquisition rate was 92.8%. The overall sensitivity, specificity and diagnostic accuracy were 90.8%, 100% and 92.2% respectively. With regards to tissue sample adequacy, the tissue acquisition rates for 20G (n=114), 22G (n=37) and 25G (n=16) FNB needles were 92.9%, 89% and 100% respectively. The 20G needle had a 90.7% sensitivity and a diagnostic accuracy of 91.2%, compared to 22G (sensitivity, 90%; accuracy, 91.9%) and 25G (sensitivity, 93.8%; accuracy 93.8%) needles.</p><p><b><i>Conclusion:</i></b> The diagnostic performance of EUS-guided FNB of solid pancreatic lesions performed at our endoscopic center has met the standards for both tissue acquisition and sensitivity rate ≥ 85%. Further studies are required to evaluate any significant difference in diagnostic accuracy between the different needle sizes.</p><p><b>PP-01-108</b></p><p><b>Diagnostic yield and Safety for Pancreatic Cystic Lesions: Comparison of EUS-FNA and EUS-FNB</b></p><p>Mingmei Ye<sup>1</sup>, Xiaoyu Yu<sup>1</sup>, Yawen Ni<sup>2</sup>, Qianqi Liu<sup>1</sup>, Pan Gong<sup>1</sup>, Yuanyuan Huang<sup>1</sup>, Xiaoyan Wang<sup>1</sup> and <b>Li Tian</b><sup>1</sup></p><p><sup>1</sup><i>The Third Xiangya Hospital of Central South University, Changsha, China;</i> <sup>2</sup><i>Nanchang University Queen Mary School, Nanchang, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives</i></b>: The incidence and detection rate of pancreatic cystic lesions (PCLs) is increasing in recent years. Endoscopic ultrasound (EUS) plays an irreplaceable role in the diagnosis and differential diagnosis of PCLs, but the evidence on the comparative diagnostic performance of EUS-guided fine-needle aspiration (EUS-FNA) and fine-needle biopsy (FNB) is limited. So, we aimed to compare the diagnostic yield, specimen adequacy, and safety of EUS-FNA and FNB in PCLs to help guide current clinical practice.</p><p><b><i>Materials and Methods</i></b>: Between January 2014 and August 2021, patients with PCLs who underwent EUS-FNA or FNB were retrospectively enrolled. The primary outcome was the diagnostic yield of EUS-FNA and FNB. The secondary outcomes were specimen adequacy and adverse events.</p><p><b><i>Results:</i></b> A total of 90 patients were included (52 in FNA and 38 in FNB). The diagnostic yield was similar in the FNA group compared with FNB (94.2% vs 94.7%, P>0.05). The specimen adequacy was 71.2% and 81.6% in FNA and FNB, respectively (P>0.05). In terms of adverse events, there was no statistical difference(P>0.05).</p><p><b><i>Conclusion:</i></b> EUS-FNA and EUS-FNB showed equally high diagnostic rate and specimen adequacy in PCLs with an excellent safety profile. Both FNA and FNB may be good and safe diagnostic tools for PCLs.</p><p><b>PP-01-109</b></p><p><b>Cold water infusion can improve the image quality of the miniprobe endoscopic ultrasonography under sedation</b></p><p>Xiaoyu Yu<sup>1</sup>, Mingmei Ye<sup>1</sup>, Jiangtao Liao<sup>2</sup>, Yang Hu<sup>3</sup>, Min Guo<sup>4</sup>, Bin Zeng<sup>5</sup>, Juan Li<sup>2</sup>, Liu Peng<sup>3</sup>, Wenfang Hu<sup>4</sup>, Han Liu<sup>5</sup>, Pan Gong<sup>1</sup>, Yu Long<sup>1</sup>, Xiaoyan Wang<sup>1</sup> and <b>Li Tian</b><sup>1</sup></p><p><sup>1</sup><i>The Third Xiangya Hospital of Central South University, Changsha, China;</i> <sup>2</sup><i>the Hunan Provincial People’s Hospital, Changsha, China;</i> <sup>3</sup><i>the Affiliated Nanhua Hospital of University of South China, Hengyang, China;</i> <sup>4</sup><i>the First People’s Hospital of Changde City, Changde, China;</i> <sup>5</sup><i>the First Affiliated Hospital of University of South China, Hengyang, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Miniprobe endoscopic ultrasonography (mEUS) plays an increasingly significant role in gastrointestinal (GI) tumors. Previous studies showed the water temperature could affect patients’ GI peristalsis and safety, however, the optimal water temperature for mEUS remains uncertain. We aimed to investigate the effects of different water temperatures on mEUS under sedation.</p><p><b><i>Materials and Methods:</i></b> We conducted a prospective, multicentre, double-blind, randomized study. Patients were randomly assigned to the cold (6–10 °C, CG), warm (20–24 °C, WG), and hot (35–39 °C, HG) water groups. The primary outcome was the image quality. Secondary outcomes included diagnostic accuracy, peristaltic grade, haemodynamics (T0–T5), comfort and satisfaction scores, and adverse events.</p><p><b><i>Results:</i></b> 240 patients were randomly assigned, with 80 patients in each group, between June 2022 and March 2023. The percentage of images with a quality score of 5 in the CG, WG, and HG was 88.8%, 75.0%, and 65.0%, respectively (P = 0.002); the diagnostic accuracy of the CG was higher than that of the WG and HG, although not statistically different (97.0% vs 81.5% vs 81.1%, P = 0.083). The CG had significantly lower peristaltic scores after water infusion than the WG and HG (P<0.001). No serious adverse events were observed. The comfort and satisfaction scores were similar.</p><p><b><i>Conclusion:</i></b> The CG was more advantageous than the WG and HG in reducing GI peristalsis, further improving image quality with excellent safety. Cold water (6–10 °C) may be the optimal water temperature for mEUS under sedation, with the potential to improve the diagnostic accuracy.</p><p><b>PP-01-110</b></p><p><b>Mastering Excellence: Learners' Insights into Deliberate Practice and Simulation-Based Learning</b></p><p>Tonya Kaltenbach<sup>1,2,3</sup>, Roy Soetikno<sup>1,2,3</sup>, Hasan Maulahela<sup>1,4</sup>, <b>Carlos Paolo Francisco</b><sup>1,5</sup>, Mark Anthony De Lusong<sup>1,6</sup>, Eric Yasay<sup>1,6</sup>, Enrik John Aguila<sup>1,5</sup>, Yung Ka Chin<sup>1,7</sup>, Wen-Feng Hsu<sup>1,8</sup>, Ruter Maralit<sup>6</sup>, Jonard Co<sup>5</sup>, Yasuhisa Abe<sup>1</sup> and Patricia Anne Cabral-Prodigalidad<sup>1,5,6</sup></p><p><sup>1</sup><i>Academy of Endoscopy, Woodside, United States;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, San Francisco VA Medical Center, San Francisco, United States;</i> <sup>3</sup><i>Department of Medicine, University of California, San Francisco, United States;</i> <sup>4</sup><i>Faculty of Medicine University of Indonesia - Cipto Mangunkusumo General Central National Hospital, Jakarta, Indonesia;</i> <sup>5</sup><i>Institute of Digestive and Liver Diseases, St. Luke’s Medical Center - Global City, Taguig City, Philippines;</i> <sup>6</sup><i>Department of Gastroenterology, Department of Medicine, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines;</i> <sup>7</sup><i>Mount Elizabeth Hospital, Singapore;</i> <sup>8</sup><i>Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction and Objectives:</i></b> Deliberate Practice (DP) is the most effective method to develop expertise in all disciplines. This approach allows learners to practice specific skills repeatedly until they reach a predetermined level of mastery. Simulation-Based Mastery Learning (SBML) is a stringent form of competency-based education that leverages the principles of DP. By using case-based simulations, SBML creates a structured and controlled learning environment, ensuring that learners achieve a higher level of proficiency.</p><p>Traditional training methods often involve passive learning, where learners acquire theoretical knowledge without ample opportunity to practice the skills. In contrast, SBML engages learners in simulated scenarios, enabling them to gain hands-on experience in a risk-free setting. We have used SBML to provide a significantly more effective endoscopy training. During the training, learners focus primarily on mastering the content of the course. However, they are not explicitly taught the underlying principles of DP that make SBML so effective. Herein, we aim to share the learners' understanding of the details of the SBML method.</p><p><b><i>Methods and Results:</i></b> We asked gastroenterology trainees (n=32) to provide reflections on their learning experience during a colonoscopy course. These reflections were recorded and analyzed using qualitative analysis to document their understanding of the SBML method used to learn the principles of colonoscopy. From this data, we created a video to showcase their insights.</p><p><b><i>Conclusion:</i></b> Trainees of this SBML course exhibit a great understanding and appreciation of its principles. They expressed how they applied these principles effectively, allowing them to maximize their learning opportunities.</p><p><b>PP-01-111</b></p><p><b>Does artificial intelligence improves the quality of screening colonoscopy?</b></p><p><b>Sabina Guseinova</b><sup>1</sup>, Sergey Kashin<sup>1,2</sup> and Dmitry Zavialov<sup>1,2</sup></p><p><sup>1</sup><i>Yaroslavl Regional Cancer Hospital, Yaroslavl, Russian Federation;</i> <sup>2</sup><i>Yaroslavl State Medical University, Yaroslavl, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> According to WHO colorectal cancer is the third most common diagnosis in the world in 2022. The detection rates of polyps and adenomas are inversely proportional to the risk of interval colorectal cancer. They also assess the competence of an endoscopist. Deep neural network algorithms is a promising way to improve colonoscopy quality.</p><p><b><i>Materials and Methods:</i></b> In prospective single-center study it was assessed the impact of AI-program for polyp detection during the screening colonoscopy (SC), which was performed by an experienced doctor from May to December 2023. Patients who had not undergone colonoscopy before were randomly assigned into 2 groups. All detected lesions were sent for histological examination. Then it was evaluated the colonoscopy quality indicators.</p><p><b><i>Results:</i></b> The control group included 54 patients (mean age 61.7), the group of patients who had a SC with AI-program — 56 (mean age 60.9). The cecal intubation rate (CIR) was 100% both. All patients had bowel preparation more than 7 points on the BBPS; Respectively in control and study groups PDR was 0.52 and 0.56; ADR — 0.26 and 0.29; APC — 0.39 and 0.41; APP — 1.5 and 1.6; CWT — 8.39 and 11.48.</p><p><b><i>Conclusion:</i></b> The use of the AI-software insignificantly improved colonoscopy quality indicators by qualified doctor, but significantly increased the colonoscopic withdrawal time. A comparative study will be conducted with the participation of endoscopists with less than 3 years of experience. This will reveal whether the AI-program can enhance the quality of colonoscopy performed by inexperienced endoscopists.</p><p><b>PP-01-112</b></p><p><b>Endoscopic interventions for malignant afferent loop syndrome: Which is the best treatment?</b></p><p><b>Tomohiro Kaketani</b></p><p><i>Tokyo Medical University Hospital, Shinjuku, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Afferent loop syndrome (ALS) is a life-threatened adverse event after gastrointestinal surgery requiring appropriate early decompression treatment. Several endoscopic interventions have been attempted for treatments such as endoscopic enteral metal stent placement (EMSP), endoscopic ultrasound (EUS)-guided entero- enterostomy (EUS-EE), and EUS-guided hepaticogastrostomy (EUS- HGS). However, there are limited data on outcomes, including duration of stent patency. Therefore, we evaluated the usefulness of each endoscopic intervention for malignant ALS.</p><p><b><i>Materials and Methods:</i></b> We retrospectively collected data of 3 ALS patients each whom underwent EMSP, EUS-EE, and EUS-HGS. Information on technical success, clinical efficacy, adverse events, stent dysfunction, and overall survival was gathered and analyzed.</p><p><b><i>Results:</i></b> All of the stent placement has done successfully, without any fatal event. Abdominal pain and cholangitis were the most frequent symptoms. Throughout the study, 2 patients treated by EUS-HGS suffered from stent dysfunction, 8 patients died of primary disease during a median follow-up of 157 days.</p><p><b><i>Conclusions:</i></b> Choosing any of the endoscopic interventions for malignant ALS can be expected to produce similar outcomes, including duration of stent patency. The choice of endoscopic intervention should be made based on the characteristics of each treatment.</p><p><b>PP-01-113</b></p><p><b>Management of Chronic Hemorrhagic Radiation Proctitis: From Six Cases Treated with Endoscopic Argon Plasma Coagulation</b></p><p><b>Ida Mujahidah Kamaruddin</b><sup>1,2</sup>, Muhammad Luthfi Parewangi<sup>1,2</sup>, Fardah Akil<sup>1,2</sup>, Nu'man AS Daud<sup>1,2</sup>, Rini Rachmawarni Bachtiar<sup>1,2</sup>, Susanto Hendra Kusuma<sup>1,2</sup>, Amelia Rifai<sup>1,2</sup> and Resha Dermawansyah Rusman<sup>1,2</sup></p><p><sup>1</sup><i>Division of Gastroentero-hepatology, Department of Internal medicine, Faculty of medicine, Hasanuddin University, makassar, Indonesia;</i> <sup>2</sup><i>Centre of Gastroenterology-Hepatology HAM Akil, DR. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> A common treatment for gynecological cancers is radiation therapy, it can be helpful in treating cancers, but also cause collateral GI tract damage. While acute toxicity can manifest as proctitis or enteritis, the more concerning sign is the development of a chronic hemorrhagic radiation proctitis (CHRP). The hallmarks of CHRP included mucus discharge, tenesmus, hematochezia, and occasionally fecal incontinence. Endoscopic argon plasma coagulation (APC) therapy stops or significantly reduces rectal bleeding in 79% to 100% of patients whose rectal bleeding is caused by CHRP.</p><p><b><i>Case Illustration:</i></b> Six cases that were initially evaluated at Dr Wahidin Sudirohusodo Hospital, Makassar, Indonesia. Each patient had pelvic radiation treatment for their malignancy, and they were all referred for ongoing transfusion-dependent hemorrhage due to radiation proctitis. The rectum has telangiectasis caused by radiation. After one to three APC Erbe Vio 200 S therapy sessions with a gas flow of 2.0 l/min and a temperature range of 40–60 W, all patients saw a significant reduction in their symptoms. After treatments, neither patient needed blood transfusions, and their hemoglobin levels improved. At the 3- and 6-month follow- up, they were both doing well.</p><p><b><i>Discussion:</i></b> Radiation therapy, while effective against cancers like gynecological cancers, can lead to collateral damage in the GI tract, such as CHRP. This case illustration demonstrated that APC therapy successfully reduced rectal bleeding in patients with radiation-induced proctitis and eliminating the need for transfusions, with sustained benefits observed at follow-up appointments. We recommend colonoscopy screening for patient who underwent pelvic radiation therapy.</p><p><b>PP-01-114</b></p><p><b>LOCALLY ADMINISTERED PHOTODYNAMIC THERAPY FOR CANCER USING NANO=ADHESIVE PHOTOSENSITIZER</b></p><p><b>Yoshiki Komatsu</b><sup>1</sup> and Toru Yoshitomi<sup>2</sup> and Hirofumi Matsui</p><p><sup>1</sup><i>University of Tsukuba Hospital, Tsukuba, Japan;</i> <sup>2</sup><i>National Institute for Materials Science, Tsukuba, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Photodynamic therapy (PDT) is a great potential anti-tumor therapy owing to its non-invasiveness and high spatiotemporal selectivity. However, systemically administered photosensitizers diffuse in the skin and the eyes for a long duration, which cause phototoxicity to bright light and sunlight. Therefore, following PDT, patients must avoid exposure of to light and sunlight to avoid this phototoxicity. In this study, we have developed a locally administered PDT using nano-adhesive porphyrin with polycations consisting of quaternary ammonium salt groups (aHP) as a photosensitizer. The aHP, approximately 3.0 nm in diameter, adhered the negatively charged cell membrane via electrostatic interaction. The aHP localized to the endosome via cell adhesion and induced apoptosis upon 635 nm light irradiation. On being administered subcutaneously on the tumor, 30% of the injected aHP remained in the administered sites. However, low-molecular-weight hematoporphyrin dihydrochloride (HP) disappeared due to rapid diffusion. PDT with locally administered aHP showed a higher anti-tumor effect after light irradiation at 635 nm for three days compared to low-molecular-weight HP. Intraperitoneal administration of HP caused severe phototoxicity upon irradiation with ultraviolet A at 10 J cm-2, whereas aHP did not cause phototoxicity because its diffusion into the skin could be suppressed, probably due to the high-molecular weight of aHP. Therefore, locally administered PDT with aHP is a potential PDT having high therapeutic efficacy without phototoxicity.</p><p><b>PP-01-115</b></p><p><b>A Meta-analysis on Endoscopic Gastric Balloon Placement versus Endoscopic Sleeve Gastroplasty for Management of Obesity</b></p><p><b>Natasha Bernadine Mapa</b> and Marc Julius Navarro</p><p><i>Makati Medical Center, Makati City, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Managing obesity that does not respond to lifestyle change or pharmacotherapy poses significant challenges, especially in determining the best intervention. The situation emphasizes the challenge of maintaining weight loss over time and the importance of using comprehensive strategies to effectively address obesity. In the recent years, bariatric surgery, such as gastric bypass and sleeve gastroplasty, offers significant weight loss and metabolic advantages, especially for those with severe obesity or obesity-related health conditions.</p><p><b><i>Methodology:</i></b> A meta-analysis was performed using 4 studies which directly compared the effectiveness of endoscopic gastric balloon (EGB) placement versus endoscopic sleeve Gastroplasty (ESG) for management of obesity. Electronic databases such as The Cochrane Library, Pubmed, Google Scholar, and Science Direct were searched for relevant studies on May 01 2024 using the search strategy: (gastric balloon placement) AND (sleeve Gastroplasty) AND (obesity). Random effects meta-analysis was performed to compute for mean difference and risk ratio to pool individual study data.</p><p><b><i>Results:</i></b> The pooled mean difference showed that ESG is associated with higher %TBWL than EGB at 1 month (MD=-2.3, 95%CI=-4.1 to -0.6, p=0.009) and at 12 months (MD=-7.6, 95%CI=-10.8 to -4.5, p<0.00001). No significant difference was observed in the 6 months period (MD=-2.3, 95%CI=-5.3 to 0.7) and in terms of adverse events (RR=1.6, 95%CI= 0.2 to 12.8).</p><p><b><i>Conclusion:</i></b> We observed a higher benefit of ESG for weight loss compared to EGB among obese individuals. Both interventions are equally safe and not associated with serious adverse events.</p><p><b>PP-01-116</b></p><p><b>Acute pancreatitis from compressive effect of intragastric balloon auto-inflation due to Candida infection</b></p><p><b>Yan Ling Ong</b><sup>1</sup>, R Rajesh<sup>1,2</sup>, Carlos Paolo Francisco<sup>3</sup> and Ravishankar Asokkumar<sup>1,2</sup></p><p><sup>1</sup><i>Gastroenterology and Hepatology, Singapore General Hospital, Singapore;</i> <sup>2</sup><i>DUKE- NUS Graduate Medical School, Singapore;</i> <sup>3</sup><i>Institute of Digestive and Liver Diseases, St Luke’s Medical Centre-Global City, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Obesity is a metabolic disorder with considerable health implications affecting populations worldwide. Intragastric balloon (IGB) placement has gained popularity in the recent years as a less invasive and reversible management option for Obesity in comparison to bariatric surgery. IGBs are safe but can have rare adverse outcomes.</p><p><b><i>Case Description:</i></b> We present a rare case of IGB auto-inflation due to Candida infection that resulted in local compressive effects on the pancreas and resulted in pancreatitis. Urgent endoscopic removal of the balloon resulted in complete resolution of the pancreatitis and symptoms.</p><p><b><i>Discussion:</i></b> Spontaneous balloon hyperinflation resulting in local compression on the pancreas has been described to be related to bacterial and fungal overgrowth. The management of complications related to IGB placement including IGB-induced acute pancreatitis typically involves early recognition and balloon removal to prevent further progression.</p><p><b>PP-01-117</b></p><p><b>Post procedural pain management following endoscopic sleeve gastroplasty</b></p><p><b>Yan Ling Ong</b><sup>1</sup>, Charlene Tan Hui Ru<sup>1</sup>, R Rajesh<sup>1,2</sup>, Carlos Paolo Francisco<sup>3</sup> and Ravishankar Asokkumar<sup>1,2</sup></p><p><sup>1</sup><i>Gastroenterology and Hepatology, Singapore General Hospital, Singapore;</i> <sup>2</sup><i>DUKE- NUS Graduate Medical School, Singapore;</i> <sup>3</sup><i>Institute of Digestive and Liver Diseases, St Luke’s Medical Centre-Global City, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There is growing concern that patients undergoing bariatric surgery are exposed to excessive opioids post-procedure with studies showing 6-10% of opioid naïve patients developing chronic dependence post-operatively. Endoscopic sleeve gastroplasty (ESG) is a minimally invasive alternative to bariatric surgery that is gaining popularity but currently, there is no established guideline on post-procedural pain management. The data on opioid use and dependance post-ESG is scant.</p><p><b><i>Methods:</i></b> This was a retrospective study of patients who underwent ESG or revision-ESG in a tertiary medical center in Singapore from January 2020 to July 2024. The primary outcome was the number of patients requiring post-operative opioid therapy. Secondary outcomes included frequency and dosage of post-operative pain medications and post-operative pain scores.</p><p><b><i>Results:</i></b> Of the 194 patients who underwent ESG or R-ESG, 62 patients (31.9%) developed post-procedural abdominal pain with a mean pain score of 3.4. These patients required low dose intravenous pethidine and paracetamol limited to one day while inpatient. They did not require post-operative patient-controlled analgesia (PCA) or further opioids. On discharge, they were given only one week of per requirement panadeine (paracetamol + codeine) and none required chronic analgesia thereafter. The mean amount of opioids prescribed expressed as morphine milligram equivalents (MME) is 10 MME on day 1 and 3.6 MME for 1 week, considerably less than post-bariatric surgery. None of the patients developed chronic opioid dependence.</p><p><b><i>Conclusion:</i></b> ESG is a safe procedure with minimal post-procedure pain requiring limited use of analgesia including opioids, thereby mitigating the risk of chronic opioid dependence.</p><p><b>PP-01-118</b></p><p><b>Collagenous Ileitis: Rare Condition with Diagnostic Challenges</b></p><p><b>Fei Yang Pan</b><sup>1,2</sup> and Ahmed Alrubaie<sup>3</sup></p><p><sup>1</sup><i>Macquarie University Hospital, Sydney, Australia;</i> <sup>2</sup><i>Macquarie University, Sydney, Australia;</i> <sup>3</sup><i>Bankstown-Lidcombe Hospital, Sydney, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Collagenous ileitis (CI) is a rare condition characterized by the thickening of the subepithelial collagen band in the ileum, presenting with symptoms such as diarrhea, altered bowel habits, and abdominal pain. CI is often under-recognized and challenging to diagnose due to its rarity and the need for advanced diagnostic procedures.</p><p><b><i>Case Description:</i></b> A 77-year-old female presented with recurrent melena and iron deficiency anaemia. Anti-gliadin and anti-tissue transglutaminase IgA, IgG and stool MCS tests were negative. Faecal calprotectin was elevated at 213 μg/g, with negative ANCA and ASCA. Video capsule endoscopy and antegrade double-balloon endoscopy revealed abnormal nodular mucosa in the distal jejunum and proximal ileum. Biopsies of the proximal ileum showed mildly oedematous ileal mucosa with broadening of the villi and a patchy increase in the subepithelial collagen plate between 10 and 20 μm, establishing a diagnosis of CI. Treatment with 9 mg of budesonide daily led to symptom improvement. Follow-up colonoscopy is planned to reassess the condition and determine the duration of steroid therapy.</p><p><b><i>Discussion:</i></b> CI remains a rare and under-recognized condition, often requiring double-balloon endoscopy for diagnosis. This case highlights the difficulty in diagnosing CI, particularly when the terminal ileum is not involved. Given that intermittent diarrhea is a common symptom, collagenous disorders of the small bowel should be considered in cases of refractory diarrhea. The patient’s improvement with steroids suggests a better prognosis compared to other collagenous disorders like collagenous sprue. Further research is essential to understand CI's pathogenesis and establish diagnostic and treatment guidelines.</p><p><b>PP-01-119</b></p><p><b>Fluoroscopy-Guided Endoscopic Balloon Dilatation for Corrosive Esophageal Stricture with Distal-to-Proximal Technique: A Prospective Case-Series Study</b></p><p><b>Sri Rahayu Paputungan</b><sup>1,2</sup>, Muhammad Luthfi Parewangi<sup>1,2</sup>, Fardah Akil<sup>1,2</sup>, Nu'man AS Daud<sup>1,2</sup>, Rini Rachmawarni Bachtiar<sup>1,2</sup>, Susanto Hendra Kusuma<sup>1,2</sup> and Amelia Rifai<sup>1,2</sup></p><p><sup>1</sup><i>Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>2</sup><i>Centre of Gastroenterology-Hepatology HAM Akil, DR Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Background:</i></b> The corrosive esophageal stricture is the most common sequela of caustic ingestion, significantly impairing the physical and socioeconomic abilities of patients and posing a major therapeutic challenge. Endoscopic dilatation is the first-line management option. Corrosive strictures can affect every esophageal segments, often being multiple, long, irregular, and more resistant to dilation compared to other causes of benign stricture. Fluoroscopic-guided esophageal balloon dilation is safe and easy to perform, provided the operators are aware of potential complications and the procedure is properly tailored to the patient's underlying condition. Among the therapeutic options for enhancing the effects of endoscopic dilation, one novel technique is the intralesional injection of corticosteroids.</p><p><b><i>Case report:</i></b> This case series study involves three patients with esophageal strictures due to corrosive injury who underwent fluoroscopic endoscopic balloon dilation at Wahidin Sudirohusodo Hospital. The procedure involved four to eight weekly dilations using a fluoroscopy-guided distal-to-proximal technique with a 15-20 mm balloon dilator, followed by a 40 mg intralesional injection of triamcinolone, totalling 26 procedures. The clinical targets were the relief in dysphagia and increasing body weight. All esophageal strictures were successfully dilated with no significant adverse events.</p><p><b><i>Discussion:</i></b> Fluoroscopic endoscopic balloon dilatation using a distal-to-proximal technique was performed on three patients with long esophageal strictures due to corrosive injury. This technique reduced the procedure time, and can be performed in long, multiple strictures. Steroid injection may be helpful in reduced of required dilatation session.</p><p><b>PP-01-120</b></p><p><b>Comparative study protocol on the efficacy of remimazolam and midazolam in endoscopic sedation in Japan</b></p><p><b>Daisuke Yamaguchi</b><sup>1</sup>, Ryoji Ichijima<sup>2</sup>, Hisatomo Ikehara<sup>3</sup>, Yosuke Minoda<sup>4</sup>, Mitsuru Esaki<sup>4</sup>, Ayako Takamori<sup>5</sup>, Yutaro Fujimura<sup>1</sup>, Ryo Shimoda<sup>6</sup> and Motohiro Esaki<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan;</i> <sup>2</sup><i>Department of Gastroenterology, Saitama Saiseikai Kawaguchi General Hospital, Saitama, Japan;</i> <sup>3</sup><i>Department of Gastroenterology, Internal Medicine, Kitasato University School of Medicine, Sagamihara, Japan;</i> <sup>4</sup><i>Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan;</i> <sup>5</sup><i>Clinical Research Center, Saga University Hospital, Saga, Japan;</i> <sup>6</sup><i>Department of Endoscopic Diagnostics and Therapeutics, Saga University, Saga, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The need for sedation in gastrointestinal endoscopy is increasing and has become a highly important medical procedure in daily practice. Remimazolam is a newly developed, ultra-short-acting benzodiazepine and has a shorter pharmacokinetic half-life than midazolam. It is expected to shorten the time to awaken after an endoscopy and the time to leave the recovery room, making it useful for sedation in the endoscopy field. This prospective study (RECOVER study) was designed based on the hypothesis that remimazolam would be useful as a sedative agent in gastrointestinal endoscopy in Japanese patients.</p><p><b><i>Materials and Methods:</i></b> This multicenter, randomized, controlled trial in Japan randomized patients aged 18-80 years undergoing upper gastrointestinal endoscopy or colonoscopy with sedation to either the remimazolam or midazolam group. The primary outcome will be the percentage of study patients who are ambulatory 5 minutes after the endoscopy. The secondary outcomes will be the success rate of sedation with a Modified Observer's Assessment of Alertness/Sedation (MOAA/S) score ≤ 4 before endoscopy and the dose of remimazolam and midazolam required to achieve sedation (MOAA/S score ≤ 4), etc. The sample size was calculated to be 80 cases (40 upper gastrointestinal endoscopies and 40 colonoscopies) with reference to the pilot study.</p><p><b><i>Results:</i></b> The study is ongoing. (jRCT pending)</p><p><b><i>Conclusions:</i></b> This study aimed to design a study comparing the sedative effects of remimazolam and midazolam in gastrointestinal endoscopy in Japanese patients and to provide evidence that remimazolam is useful for sedation in the endoscopy field.</p><p><b>PP-01-121</b></p><p><b>Revisiting duodenal mucosal resurfacing in treatment of type 2 diabetes melllitus: An updated meta-analysis</b></p><p><b>Ahmad Fariz Malvi Zamzam Zein</b><sup>1,2</sup>, Ayu Oktaviana<sup>3</sup>, Edi Mulyana<sup>1</sup>, Nikko Darnindro<sup>1</sup>, Annela Manurung<sup>1</sup>, Marina Epriliawati<sup>4</sup>, Muhammad Ikhsan Mokoagow<sup>4</sup>, Jerry Nasarudin<sup>4</sup> and Anak Agung Arie Widiastuti<sup>4</sup></p><p><sup>1</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;</i> <sup>3</sup><i>Faculty of Medicine, Trisakti University, Jakarta, Indonesia;</i> <sup>4</sup><i>Division of Endocrinology, Metabolism, and Diabetes, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This meta-analysis was performed to emphasize the benefit and safety of duodenal mucosal resurfacing (DMR) among patients with type 2 diabetes mellitus (T2DM) based on the most current evidence.</p><p><b><i>Materials and Methods:</i></b> Cochrane Library, MEDLINE, Proquest, Pubmed, Scopus, and ScienceDirect were systematically searched reporting the outcome of DMR among patients with T2DM and published up to April 15th, 2024. Data extraction was performed independently by authors. The primary outcome was metabolic improvement including HbA1c, body weight (BW), and HOMA-IR. The secondary outcome was safety profile of DMR encompassing severe adverse event (SAE). Heterogeneity was examined using I² statistics and a random-effects model was employed. Review Manager 5.4.1 was used for statistical analysis.</p><p><b><i>Results:</i></b> There were 7 studies recruiting a total 298 patients. All studies were prospective clinical studies. Six studies reported change in HbA1c in six months and one study reported at three months. There was significant HbA1c reduction in patients with DMR compared to baseline by 5.18% (95% CI -6.90, - 3.46, p <0.001). Additionally, DMR was associated with BW reduction by 0.28 kg (95% CI -0.47, -0.09, p <0.001) and decreased HOMA-IR by 0.91 (95% CI -1.19, -0.63). There were three out of 118 patients (2.54%) had SAE with an effect size of 1.67.</p><p><b><i>Conclusion:</i></b> Performing DMR provides beneficial metabolic outcomes in management of T2DM. It is also a harmless endoscopic approach for patients with T2DM.</p><p><b>PP-01-122</b></p><p><b>Obscure bleeding from a metastatic small bowel tumor diagnosed using motorized spiral enteroscopy: case report</b></p><p><b>Christian Banciu</b> and Andreea Munteanu</p><p><i>University of Medicine and Pharmacy TImisoara/IV Department of Internal Medicine, Timisoara, Romania</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Small bowel tumors are relatively rare, representing only around 5% of all gastrointestinal neoplasms, with a progressively increasing incidence. Currently, there are no established guidelines for diagnostic approaches, screening procedures, or management strategies for small bowel tumors.</p><p><b><i>Case desciption:</i></b> We present here the case of a patient with a rare type of metastatic tumor of the small bowel originating from primary lung adenocarcinoma who presented with abdominal pain, severe iron-deficiency anemia, and melena. The initial investigations, gastroscopy and colonoscopy, failed to identify the bleeding source. The obscure bleeding source and diagnosis were achieved through power motorized spiral enteroscopy (MSE), which allowed the visualization and biopsy of the tumor. Histopathological examination established the presence of a poorly differentiated non-mucinous adenocarcinoma originating from the lung.</p><p><b><i>Discussion:</i></b> although the MSE was abandoned world wide, we report this case to provide evidence of the efficiency of MSE in the diagnosis of small bowel tumors, with the method providing higher insertion depth in a reduced amount of time. In experienced hands we suggest that MSE still represents a safe and more time saving method than other types of enteroscopy, allowing to perform diagnostic and therapeutic procedures on the small bowel. Off course, further technical improvements can and must be obtain on this endoscopy method (MSE) in order to make it more safe and reliable.</p><p><b>PP-01-123</b></p><p><b>Clinical Usefulness and Acceptability of Small-bowel Capsule Endoscopy with Panoramic Imaging Compared with Axial Imaging</b></p><p><b>Issei Hirata</b>, Akiyoshi Tsuboi, Shuya Shigenobu, Yuka Matsubara, Takeshi Takasago, Tomoyuki Nishimura, Hidenori Tanaka, Ken Yamashita, Yuichi Hiyama, Hidehiko Takigawa, Yuji Urabe, Toshio Kuwai and Shiro Oka</p><p><i>Hiroshima University Hospital, Hiroshima, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate the usefulness and acceptability of CapsoCam Plus® (CapsoCam).</p><p><b><i>Materials and Methods:</i></b> This retrospective single-center study enrolled 930 patients with suspected small-bowel bleeding (SSBB) who underwent capsule endoscopy. Thirty-three patients using CapsoCam and PillCamTM SB3 (SB3) were matched using the propensity score matching. The diagnostic yield was evaluated by comparing both groups and the acceptability of CapsoCam use.</p><p><b><i>Results:</i></b> There was no SSBB case where capsule endoscopy was performed within 48 h of bleeding. CapsoCam had a significantly higher observation rate of the entire small-bowel (97% vs. 73%, P = 0.006) and Vater’s papilla (82% vs. 15%, P < 0.001) than SB3. The reading time of CapsoCam was significantly longer than that of SB3 (30 vs. 25 min, P < 0.001), and CapsoCam’s time from the capsule endoscopy swallowing to read completion was also longer than that of SB3 (37 vs. 12 h, P < 0.001). The two groups showed no difference in the capsule endoscopy findings according to P classification. Notably, 85% of the patients using CapsoCam reported examination distress as \"not at all\" or \"almost not,\" and 94% reported swallowing difficulty as \"very easy\" or \"easy.”</p><p><b><i>Conclusion:</i></b> CapsoCam took time to read; however, it is a well-tolerated examination with a high observation rate of Vater’s papilla and entire small-bowel mucosa. Detectability of bleeding sources was comparable in both modalities for cases of occult SSBB and overt SSBB more than 48 hours after bleeding. CapsoCam is a useful modality for patients with SSBB.</p><p><b>PP-01-124</b></p><p><b>Clinical Outcomes of Delayed Capsule Endoscopy in Inpatients with Small Bowel Bleeding:Propensity Score Matching Analysis</b></p><p><b>Seung Min Hong</b><sup>1</sup>, Dong Hoon Baek<sup>1</sup>, Cheolung Kim<sup>1</sup>, Hong Sub Lee<sup>2</sup>, Geun Am Song<sup>1</sup>, Hyeon Tae Cho<sup>3</sup>, Jeong Gil Park<sup>4</sup>, Gwang Ha Kim<sup>1</sup>, Bong Eun Lee<sup>1</sup>, Moon Won Lee<sup>1</sup> and Dong Chan Joo<sup>1</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea;</i> <sup>2</sup><i>Inje University Busan Paik Hospital, Busan, South Korea;</i> <sup>3</sup><i>Centum Medihill Hospital, Busan, Republic of Korea;</i> <sup>4</sup><i>Kimsan Internal Medicine Clinic, Sacheon, Republic of Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This retrospective study analyzes the clinical outcomes of performing delayed capsule endoscopy in patients hospitalized for small bowel bleeding.</p><p><b><i>Materials and Methods:</i></b> All patients were divided into two groups: the Early-48 group (n = 46), who underwent capsule endoscopy within 48 hours of the bleeding episode, and the Late-48 group (n = 54), who underwent capsule endoscopy more than 48 hours after the bleeding episode. Using 1:1 propensity score matching (PSM) analyses, 34 pairs were made.</p><p><b><i>Results:</i></b> After the 1:1 PSM, there was no difference in yield for both groups to detect positive findings on capsule endoscopy, the need for intensive care unit (ICU) care, the amount of blood transfusion, and the number of recurrent bleeding episodes (Table 1). When the patients were divided into two groups based on 72 hours and after performing 1:1 PSM, the group that underwent capsule endoscopy within 72 hours of a bleeding episode had a significantly higher yield in detecting positive findings compared to the group that underwent capsule endoscopy more than 72 hours after a bleeding episode. (95.5% vs. 68.2%, p = 0.046). However, there were no differences in other clinical outcomes between the two groups.</p><p><b><i>Conclusion:</i></b> Although performing delayed capsule endoscopy on inpatients with small bowel bleeding may reduce the yield in detecting positive findings, it is considered feasible as it does not affect patient survival and the outcomes of inpatient treatment.</p><p><b>PP-01-125</b></p><p><b>Significance of Surveillance for Small bowel Tumor with balloon-assisted enteroscopy in Familial Adenomatous polyposis (FAP)</b></p><p><b>Kentaro Iwata</b><sup>1</sup>, Kaoru Takabayashi<sup>3</sup>, Motoki Sasaki<sup>1</sup>, Yuri Imura<sup>2</sup>, Shoma Murata<sup>2</sup>, Daisuke Minezaki<sup>1</sup>, Kurato Miyazaki<sup>1</sup>, Teppei Masunaga<sup>3</sup>, Mari Mizutani<sup>3</sup>, Teppei Akimoto<sup>1</sup>, Yusaku Takatori<sup>1</sup>, Michiko Nishikawa<sup>1</sup>, Hideomi Tomida<sup>1</sup>, Noriko Matsuura<sup>1</sup>, Atsushi Nakayama<sup>1</sup>, Takanori Kanai<sup>2</sup>, Naohisa Yahagi<sup>1</sup> and Motohiko Kato<sup>3</sup></p><p><sup>1</sup><i>Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan;</i> <sup>2</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;</i> <sup>3</sup><i>Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The pathophysiology of small bowel (SB) tumors in patients with familial adenomatous polyposis (FAP) remains unclear, and surveillance methods have not yet been established. Therefore, the purpose of this study was to investigate the significance of SB surveillance with balloon-assisted enteroscopy (BAE).</p><p><b><i>Materials and Methods:</i></b> We analyzed the severity of SB tumors and their correlation with disease severity in other organs from 30 patients associated with FAP who underwent BAE for SB from October 2023 to June 2024. The severity of SB tumors was recorded every 30 cm from the Treitz ligament in the upper SB and from the ileocecal valve or the postoperative anastomosis in the lower SB.</p><p><b><i>Results:</i></b> The mean age of the patients was 49 years, and 78% of the patients had undergone total colectomy. The average of insertion depth of trans-oral enteroscopy was 102 cm from Treitz ligament, and lesions were observed in 55% of the patients. The lesions were presented 48%/23%/19% in each part of the upper SB (30 cm/30-60 cm/60-90 cm from Treitz ligament). Large lesions of more than 15 mm were observed in 16% of cases. Trans-anal enteroscopy was performed in 7 patients, and lesions were observed in 13% of patients. No correlation was found between the presence or absence of upper SB lesions and the severity of gastric or duodenal polyposis.</p><p><b><i>Conclusion:</i></b> SB surveillance with BAE should be performed in patients with FAP, regardless of the severity of other gastrointestinal tumors.</p><p><b>PP-01-126</b></p><p><b>Evaluating the role of small intestine dual energy CT and double-balloon endoscopy in Crohn’s disease</b></p><p><b>Yanjun Chen</b>, Ji Liu and Fujuan Luan</p><p><i>The First Affiliated Hospital Of Soochow University, Suzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study seeks to evaluate the role of small intestine DECT and DBE in the diagnosis of CD.</p><p><b><i>Materials and Methods:</i></b> The study included 72 CD patients (4 patients underwent both DECT and DBE twice, totally, 76 CD patients’ data) who underwent both small intestine DECT and DBE within 3 months and were hospitalized in the First Affiliated Hospital of Soochow University from July 1, 2016, to November 1, 2023.</p><p><b><i>Results:</i></b> Among 76 CD patients’ data, there were 51 males and 25 females, with an age of (38.86 ± 12.05) years old. CD detection rate by DBE was 80.3%, higher than 65.8% by DECT (P = 0.044). The combined CD detection rate was 89.5%, higher than DECT alone (P < 0.05). The detection rate of stenosis by DBE was 46.1%, higher than 13.2% by DECT (P < 0.05). The combined detection rate of stenosis was 52.6%, higher than DECT alone (P < 0.05). The detection rate of fistula by DBE was 3.9%, by DECT was 2.6%, and by the combined method was 5.3%. For ulcers, DBE had a higher detection rate of 73.7% compared to 7.9% of DECT (P < 0.05). The combined ulcer detection rate was 76.3%, higher than DECT alone (P < 0.05). The detection rate of long ulcers and non-ileocecal ulcers by DBE were both 17.9%.</p><p><b><i>Conclusion:</i></b> For the detection of CD, stenosis, and ulcers, DBE or combined method has an advantage over DECT. Notably, DBE has an advantage in detecting long ulcers and non-ileocecal ulcers.</p><p><b>PP-01-127</b></p><p><b>Clinical case: Small intestinal capsule endoscopy and histopathology of Patients with Eosinophilic Enteritis</b></p><p><b>Tiến sĩ Phu Pham Quang</b><sup>1</sup>, Thuan Nghiem Duc<sup>2</sup>, Thuong Nguyen Hoai<sup>1</sup>, Nhuong Duong Xuan<sup>1</sup>, Hong Nguyen Phuong<sup>1</sup>, Chi Dinh Minh<sup>1</sup>, Chung Le Ngoc<sup>1</sup>, Huong Huynh Thi Thu<sup>1</sup>, Lai Nguyen Van<sup>1</sup>, Linh Nguyen Thuy<sup>1</sup>, Thanh Thanh Nguyen Hong<sup>1</sup> and Kim Nguyen Huu<sup>1</sup></p><p><sup>1</sup><i>Military Hospital 103, Hanoi, Vietnam;</i> <sup>2</sup><i>Vietnam Military Medical University, Hanoi, Vietnam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Eosinophilic gastroenteritis (EGE), which demonstrates pathological dense eosinophil infiltration in the gastrointestinal tract irrespective of esophageal involvement. We would like to report a clinical case of eosinophilic enteritis with capsule endoscopy and histopathology.</p><p><b><i>Case Description:</i></b> 20-year-old male presented with fatigue, anorexia, abdominal pain. Hemoglobin level, 113 g/L; mean corpuscular volume, 76,7 fL), and peripheral eosinophilia (665 cells/mL). Stool examinations were negative for parasites. The diagnosis of tuberculosis infection was ruled out by the quantiferon test and on the biopsy.</p><p>Upper endoscopy were grossly and histologically unremarkable. Colonoscopy showed nothing unremarkable histologically and grossly in the colon. Inserting the scope through the Bauhin valve into the ileum detects erosive inflammation and ulceration. A biopsy of the lesion is performed showed jejunal eosinophilia with over 50 eosinophils per high-power field. (Figure A)</p><p>The image of the lesion detected through capsule endoscopy continuously from the capsule location at 3 hours 35 minutes to the end of the ileum at 9 hours 4 minutes. Redness or erythema was the most common endoscopic finding in the small bowel, followed by villous atrophy, erosion, ulceration, and edema. Other findings included mucosal congestion, whitish exudate and short-rounded edematous villi. (Figure B)</p><p><b><i>Discussion:</i></b> The characteristics of small bowel endoscopic findings on EGE remain unclear. It is hoped that this report will provide a valuable source for further clarification of the endoscopic findings of the small bowel and further elucidation of the pathogenesis of EGE through more detailed studies with a larger number of cases.</p><p><b>PP-01-128</b></p><p><b>Per-oral Endoscopic Myotomy (Z-POEM) As An Effective Treatment for Zenker's Diverticulum: A Case Report</b></p><p><b>Saskia Nursyirwan</b> and Achmad Fauzi</p><p><i>Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Zenker's diverticulum (ZD) arises from impaired cricopharyngeal (CP) muscle distensibility during deglutition (swallowing), leading to a clinical presentation characterised by dysphagia, regurgitation, aspiration, cough, and potential weight loss. The advent of flexible endoscopic ZD treatment has revolutionized the therapeutic landscape, rapidly becoming the preferred first-line modality for management of small to moderate-sized diverticula.</p><p><b><i>Case Presentation:</i></b> In this medical illustration, we report a woman, 64th years old with Zenker’s diverticulum treated with Z-POEM. She came to our clinic with dysphagia and regurgitation since 6 months prior. Diagnostic gastroscopy showed ZD of 3 cm, located 18 cm from incisors with thick septal muscle. A triangle-shaped knife created a 2-cm mucosal incision, and submucosal tunneling was made by spray coagulation. The gastroscope was advanced through the submucosal space of the esophageal lumen and the diverticulum site until the bottom of the diverticulum. The septal muscle was completely cut, immediately allowing the gastroscope to pass through easily, and the mucosal defect was closed with hemoclips.</p><p><b><i>Discussion:</i></b> Z-POEM offers a precise approach to treating Zenker's diverticulum by providing a complete visualization of the entire septal muscle. This comprehensive view minimizes the risk of incomplete myotomy, ensuring a more effective treatment.</p><p><b><i>Keywords:</i></b> Zenker's diverticulum, myotomy, third-space, Z-POEM, peroral endoscopic myotomy</p><p><b>PP-01-129</b></p><p><b>Association between the symptoms and the UGIE findings in a cohort of patients</b></p><p><b>Nirodha Abeywardhana</b>, Sanka Abeynayake and Chaminda Rajakaruna</p><p><i>Base hospital Gampola, Kandy, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Upper gastrointestinal endoscopy (UGIE) is a diagnostic as well as a therapeutic endoscopic procedure which is used to visualize from oropharynx to the 2nd part of the duodenum. It is commonly done to evaluate upper GI symptoms. This study was done to describe the association between the symptoms and the findings.</p><p><b><i>Materials and Methods:</i></b> Cross sectional descriptive study was conducted at Base Hospital Gampola, Sri Lanka using patients who had undergone UGIE over a six months period. Findings were recorded and analyzed using standard software.</p><p><b><i>Results:</i></b> 152 patients were in the sample. Mean age was 59+/-15.3 years. 86 (57%) were females. The most common symptom was dyspeptic symptoms 70 (46%), followed by dysphagia (24%), upper GI bleeding 23 (15%). Most common findings were gastritis 40 (26%) and hiatal hernia 22 (15%). Dyspeptic symptoms were associated with 37% gastritis, 22% hiatal hernia and 5% gastric masses. Dysphagia is 22% associated with esophageal masses, 16% hiatal hernia and 11% gastritis. Eleven percent of patients with dyspeptic symptoms and 16% of patients with dysphagia had normal studies. Six patients (4%) had gastric masses and 8 patients (5%) had oesophageal masses. Half of the patients with oesophageal masses and 1/3 of patients with gastric masses are from Tamil population. 16% of Tamil patients who had undergone UGIE had malignant lesions (p=0.1).</p><p><b><i>Conclusions:</i></b> The Symptoms dyspepsia, dysphagia and Upper GI bleeding have significant association with UGIE findings. Being a Tamil increases the risk of having an upper GI malignancy</p><p><b>PP-01-130</b></p><p><b>The Association between Drug Induced Gastropathy and Endoscopic Gastroduodenal Mucosal Lesions</b></p><p><b>Abida Abida</b>, Budi Widodo, Titong Sugihartono and Herry Purbayu</p><p><i>Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Drug induced gastropathy (DIG) is lesions of the gastric mucosa asscociated with negative manifestation either of drug or its metabolites as NSAIDs, aspirin, anticoagulants, glucocorticosteroids, anticancer drugs and oral iron preparations. The aim of this study to determine association between DIG and Endoscopic Gastroduodenal (EGD) mucosal lesions in patiens using NSAIDs, Glucocorticosteroids, Single / Double Antiplatelets (SAPT/DAPT) and Herbal Medicine.</p><p><b><i>Material and Methods:</i></b> The Study was a Cross-sectional Study conducted in Dr. Soetomo General Academic Hospital Surabaya period 2023-2024. Total 99 Subjects with dyspepsia symptoms were enrolled, medications history, EGD and histopathological examination were performed. Gastric mucosal erosion and ulcer were classified using modified LANZA Score. We analyzed data with CHI-SQUARE Test and p value < 0.05 was statistically significant.</p><p><b><i>Results:</i></b> EGD mucosal lesions were observed in 62 patients (63.6%) and 37 patients (37.4%) had ulcers. The modified Lanza Score Mean ±SD was 3.39 ±1.5. There were statistically significant association among Single Therapy (NSAID/ SAPT/ Corticosteroids), Dual Therapy (NSAID+Corticosteroids/DAPT) and Triple Therapy (NSAID + Corticosteroids + Herbal Medicine/NSAID+DAPT) with EGD mucosal lesions (p value = 0.0001, p value = 0.0001 and p value = 0.003 respectively). Duration of DIG administration, alcohol habit, severity subjective symptoms were contributing factors associated with EGD mucosal lesions showed statistically significant (p value = 0.0001, p value= 0.0001, p value = 0.001 respectively).</p><p><b><i>Conclusion:</i></b> Our finding suggested that Single Therapy, Dual Therapy, Triple Therapy, duration of DIG administration, Alcohol habit and severity subjective symptoms were contributing factors associated with EGD mucosal lesions.</p><p><b>PP-01-131</b></p><p><b>Duodenal tumor finding in patient with renal carcinoma</b></p><p><b>Budiman Syaeful Anwar</b> and Muhammad Firhat Idrus</p><p><i>RSCM, Central Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Renal cell carcinoma (RCC) is the most common malignant tumor of the kidney and constitutes over 90 percent of all renal malignancies. A third of the patients with RCC have metastatic disease at presentation1. Duodenal tumor is a rare case in patient with renal RCC. Most patients of duodenal metastasis from RCC present with upper gastrointestinal bleeding or obstructive symptoms, and other signs include anemia, melena, fatigue and early satiety2. Imaging remains the mainstay in RCC for diagnosis, screening, follow-up, and treatment monitoring1. Diagnosis of duodenal metastases as a cause of GI bleeding is a challenge due to its rarity and thus low index of suspicion for diagnosis3.</p><p><b><i>Case Report:</i></b> A 51-year-old male came with heavy weakness. 7 days before hospital admission patient had hematuria with blood clots. Patient diagnosed with renal tumor in 2018, but have not done any surgery due to economic and distance to referral hospital. This patient just performed a renal biopsy around two weeks ago. Patient was consulted to Gastroenterology because of melena since two days ago with hemoglobin levels only 5,5 g/dL. History of hematemesis and hematochezia was denied. EGD results revealed a duodenal mass pars d1-d2 junction.</p><p><b><i>Conclusion:</i></b> Patient with renal carcinoma who experienced digestive bleeding, iron deficiency anemia, a mass in the GI tract, or a history of RCC has possibility of gastrointestinal metastasis. Therefore, the patient should be suspected and get complete evaluation, such as biopsy and abdominal CT scan with contras can be option to confirm the definite diagnosis.</p><p><b>PP-01-132</b></p><p><b>Factors Influencing Endoscopic Treatment of Children with Esophageal Atresia after Esophagoplasty</b></p><p><b>Dmitriy Baranov</b>, Suhotskaya Anna, Valeria Kamalova, Malysheva Daria, German Goryaev, Evgeniy Solonitsyn, Vladislav Tutunnik, Sabina Seifedinova, Dmitriy Chernyh and Anna Poddymova</p><p><i>Almazov NMRC, Saint-Petersburg, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Factors Influencing Endoscopic Treatment of Children with Esophageal Atresia after Esophagoplasty.</p><p><b><i>Introduction:</i></b> The aim of this study was to investigate various aspects of treating patients with a particular pathology, including gender differences, the impact of the type of surgery, and the presence of tracheoesophageal fistula (TEF) on the duration of bougienage and treatment outcomes.</p><p><b><i>Case Description:</i></b> The study included 18 childrens (9 male and 8 female). The average duration of bougienage was 10 months, with an average of 7 sessions. Both single-stage (n = 12) and multi-stage surgeries (n = 6) were performed. The mortality rate for surgical treatment was 5%. The diameter of the stricture ranged from 2.5 mm to 7.3 mm. TEF was present in 4 out of 18 patients (78%).</p><p>Statistical analysis revealed significant differences in the duration of bougienage between male and female patients (p = 0,006, Student's t-test). Males required longer treatment.</p><p>There were no statistically significant differences in bougienage duration between patients undergoing single-stage and multi-stage surgeries (p = 0,820, Mann-Whitney U-test).</p><p>No correlation was found between the initial stricture diameter and the duration of bougienage.</p><p>The presence of TEF did not significantly affect the duration of bougienage.</p><p><b><i>Discussion:</i></b> The study identified gender differences in the duration of bougienage, with males requiring longer treatment. However, the type of surgery did not significantly influence the treatment duration. Further research is needed to better understand the impact of various factors on treatment outcomes.</p><p><b>PP-01-133</b></p><p><b>Injectable pH-responsive bioadhesive with robust and stable wet adhesion for gastric ulcer healing</b></p><p><b>Dake Chu</b></p><p><i>Xian Jiaotong University, Xi'an, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Development of bioadhesives that can be facilely delivered by endoscope and exhibit instant and robust adhesion with gastric tissues to promote artificial gastric ulcer healing remains challenging.</p><p><b><i>Materials and Methods:</i></b> In this study, an advanced bioadhesive is prepared through free radical polymerization of ionized N-acryloyl phenylalanine (iAPA) and N-[tris (hydroxymethyl) methyl] acrylamide (THMA).</p><p><b><i>Results:</i></b> The precursory polymer solution exhibits low viscosity with the capability for endoscope delivery, and the hydrophilic-hydrophobic transition of iAPA upon exposure to gastric acid can trigger gelation through phenyl groups assisted multiple hydrogen bonds formation and repel water molecules on tissue surface to establish favorable environment for interfacial interactions between THMA and functional groups on tissues. The in-situ formed hydrogel features excellent stability in acid environment (14 days) and exhibits firm wet adhesion to gastric tissue (33.4 kPa), which can efficiently protect the wound from the stimulation of gastric acid and pepsin. In vivo studies reveal that the bioadhesive can accelerate the healing of ulcers by inhibiting inflammation and promoting capillary formation in the acetic acid-induced gastric ulcer model in rats.</p><p><b><i>Conclusion:</i></b> Our work may provide an effective solution for the treatment of gastric ulcers or artificial ulcers via endoscopic injection clinically.</p><p><b>PP-01-134</b></p><p><b>Efficacy of draid endo in detecting upper gastrointestinal tract landmarks and lesions on endoscopy videos</b></p><p><b>Viet Hang Dao</b> and Duc Tran and Hoang Nguyen and Kien Dao and Giap Duong and Binh Nguyen and Tung Nguyen and Hoa Lam and Trang Nguyen and Steven Truong and Long Dao</p><p><i>Hanoi Medical University, Hanoi, Viet Nam;</i> <i>Institute of Gastroenterology and Hepatology, Hanoi, Vietnam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The application of artificial intelligence (AI) in endoscopy is increasing, especially in developing commercialized products for real-time lesions detection. However, data in developing countries is limited.</p><p><b><i>Objectives:</i></b> Evaluate the accuracy of the DrAid Endo model in detecting anatomical landmarks and lesions on upper gastrointestinal (UGI) endoscopy images and videos.</p><p><b><i>Methods:</i></b> DrAid Endo's algorithm was trained on a dataset comprising 46,524 endoscopic images without lesions and 13,342 endoscopic images of five lesions: reflux esophagitis, esophageal cancer, gastritis, gastric cancer, and duodenal ulcer. The dataset includes four light modes: white light and 3 image-enhanced modes (FICE, LCI, BLI). All images were labeled and verified by endoscopists with ≥5 years of experience. The EfficientNet-B5 model was used for anatomical landmark detection and YOLOv8 model for lesions detection. Accuracy of the DrAid Endo model was evaluated on 2583 images for UGI anatomical landmark detection and 47 endoscopic videos for UGI lesions detection. The endoscopic videos were divided into 284 positive sections (containing lesions) and 296 negative sections (containing no lesions) with a total time of 87.55 minutes and 75.23 minutes, respectively.</p><p><b><i>Results:</i></b> The anatomical landmark detection algorithm achieved 95% sensitivity and 99% specificity. As for lesions detection, sensitivity and specificity ranged from 91% to 96% for most lesions, except for gastritis. Sensitivity and specificity were highest in gastric cancer (95.6%, 95.9%, respectively) and lowest in gastritis (65.3%, 73.9%, respectively).</p><p><b><i>Conclusion:</i></b> DrAid Endo shows efficacy in detecting anatomical landmarks and some UGI lesions, especially malignant lesions.</p><p><b>PP-01-135</b></p><p><b>Hookworm infection presenting as acute massive upper gastrointestinal bleeding : A case report</b></p><p>Aryanti Ambarsari and <b>Muhamad Yugo Hario Sakti Dua</b></p><p><i>Gastroenterology Hepatology RSUD Provinsi Banten, Serang, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Chronic occult bleeding is well known in cases of hookworm infection, but acute gastrointestinal bleeding is rarely reported.1 A challenging evaluation is needed since soil-transmitted helminths are among the neglected tropical diseases.2 Hookworm infection is a curable condition and has a good prognosis of complete recovery.4</p><p><b><i>Case:</i></b> A 53-years old male, presented to ER with multiple large episodes of melena since one day prior admission. Hemoglobin dropped to 3,2 mg/dL from the previous 6,4 mg/dL. He was in hypovolemic shock, thus he admitted to the ICU and kept on an intravenous PPI and somatostatin. An upper GI endoscopy is performed after he received a 5-unit of blood transfusion. It revealed esophagitis LA grade B, antrum gastritis with multiple ulcer Forrest III, and duodenitis with an adult worm in the duodenum. The worm was removed, then 400 mg of albendazole were administered once daily for the first five days. No further massive bleeding was observed and he was stable clinically and hemodynamically.</p><p><b><i>Discussion:</i></b> An evaluation of duodenum using an endoscopy result in a more frequent diagnosis of worm infection as a cause of acute gastrointestinal bleeding.3 The multiple ulceration seen on endoscopy as the mechanical trauma of the intestinal mucosa due to the worms’ attachment and abrasion. This was contributed to blood loss in the absence of NSAID consumption. Hookworm infection that manifests clinically as acute digestive hemorrhage is infrequent. In tropical country, it is important to consider this pathology within the differential diagnosis in cases of bleeding.</p><p><b>PP-01-136</b></p><p><b>Photodocumentation protocol for UGI endoscopy as the Quality Indicator in the National Cancer Screening Program</b></p><p><b>Kseniya Filimendikova</b>, Sergey Kashin and Anastasiia Anastasiia</p><p><i>Yaroslavl Regional Cancer Hospital, Yaroslavl, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Esophageal and gastric cancer occupies one of the leading places in the structure of cancer morbidity (1,24 % and 5,3%, in 2022, respectively) and mortality (2,28% and 8,8% in 2022, respectively) in Russia. The use of a standardized photodocumentation protocol for EGD can help reduce mortality and increase the diagnosis of precancerous lesions, early esophageal and gastric cancer, and other conditions.</p><p><b><i>Materials and Methods:</i></b> A cohort of 211 random patients, who underwent an endoscopic examination, (66% female, 34% male), average age 59,6 years (18-84), diagnosed in 2023 in a referral center, was analyzed. The number of photos taken during the study, the time taken to perform the EGD were calculated and also the concordance of the referring institution's diagnosis with the study result was evaluated.</p><p><b><i>Results:</i></b> Average number of photographs in the protocol - 9, average time of EGD - 5.8 minutes. The number of matches between the referring institution's diagnosis and the endoscopic examination reports – 27%. The number of diagnoses, that did not match – 31,8%. The number of cases where the data cannot be evaluated — 41,2%.</p><p><b><i>Conclusion:</i></b> The data presented demonstrate the need for a photodocumentation protocol for quality control of endoscopic examination.</p><p><b>PP-01-137</b></p><p><b>Clinically significant endoscopic findings in outpatients with dyspepsia without alarm features</b></p><p><b>Taufik Hidayat</b><sup>1</sup>, Putut Bayupurnama<sup>2</sup>, Neneng Ratnasari<sup>2</sup>, Fahmi Indrarti<sup>2</sup> and Catharina Triwikatmani<sup>2</sup></p><p><sup>1</sup><i>Trainee in Division of Gastroenterology and Hepatology, Internal Medicine Department, Faculty of Medicine, Public Health and Nursing Gadjah Mada University; Dr. Sardjito Hospital, Yogyakarta, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology and Hepatology, Internal Medicine Department, Faculty of Medicine, Public Health and Nursing Gadjah Mada University; Dr. Sardjito Hospital, Yogyakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Some guidelines recommend a non-endoscopic approach in dyspepsia patients without alarm features, but several studies have shown significant endoscopic findings (SEF) in this group of patients with varying prevalence.</p><p><b><i>Objective:</i></b> This study aims to determine the prevalence and factors associated with SEF in outpatients with dyspepsia without alarm features.</p><p><b><i>Material and Methods:</i></b> We did retrospective study for outpatients with dyspepsia without alarm features who underwent endoscopy at Sardjito General Hospital Yogyakarta, between January 2023-April 2024. Demographic, symptom, BMI, and endoscopy data were collected from medical records. We defined SEF as peptic ulcer, erosive esophagitis (LA grade B and higher), malignancy, stricture, or findings required specific therapy.</p><p><b><i>Results:</i></b> A total of 110 outpatients with dyspepsia without alarm features were included in this study. The median age of participants was 43 (18-72), male 66 (60%), female 44 (40%). We had SEF in 21 (19.1%) of which peptic ulcer was 16, hiatal hernia was 5, erosive esophagitis LA grade B was 2 patients. The ≥ 50 years was statistically associated with SEF (OR = 3,196; p=0.033).</p><p><b><i>Conclusion:</i></b> The prevalence of SEF in outpatients with dyspepsia without alarm features was 19%, with peptic ulcer being the most common finding. The ≥ 50 years are associated with higher risk of SEF.</p><p><b><i>Keywords:</i></b> Dyspepsia, alarm features, significant endoscopic findings</p><p><b>PP-01-138</b></p><p><b>Successful closure with Four Tracheal and Esophageal Stents for Esophagobronchial Fistula caused by Esophageal Cancer</b></p><p><b>Ryusuke Kato</b><sup>1</sup>, Yohei Yamamoto<sup>1</sup>, Masamitsu Sotozono<sup>1</sup> and Miku Sato<sup>2</sup></p><p><sup>1</sup><i>Sapporo Teishinkai Hospital, Sapporo-shi, Japan;</i> <sup>2</sup><i>Obihiro Kousei Hospital, Obihiro-shi, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Esophagobronchial fistulas caused by esophageal cancer in the cervical esophagus are notoriously refractory, presenting significant treatment challenges.</p><p><b><i>Case Description:</i></b> A man in his 50s was diagnosed with stage IVa esophageal cancer with tracheal invasion and cervical lymph node metastasis. Despite chemoradiotherapy and systemic chemotherapy with re-irradiation, disease progression resulted in an esophagobronchial fistula, necessitating the placement of two airway stents. One month later, esophageal stenosis occurred due to compression from the airway stents, requiring an additional esophageal stent. A fully covered self-expandable metal stent with short flares, designed for the cervical esophagus, was placed. The patient maintained a stable condition for nearly six months, but the progression of the esophageal cancer led to the expansion of the esophagobronchial fistula, necessitating additional airway stents. Ultimately, four stents were used, allowing the patient to maintain quality of life for nearly a year despite the high-risk location and persistent stent treatment challenges in the cervical esophagus.</p><p><b><i>Discussion:</i></b> Despite the refractory nature of the esophagobronchial fistula due to esophageal cancer progression, persistent stent treatment proved to be a viable therapeutic option. The high-risk location in the cervical esophagus typically poses a risk for reduced quality of life, yet this patient maintained a relatively stable condition and quality of life for an extended period. This case underscores the potential benefits of aggressive stent therapy in managing complex esophageal cancer complications, even in high-risk anatomical regions.</p><p><b>PP-01-139</b></p><p><b>ASD Closure Devices: An Effective Way of Managing Leakage Following Laparoscopic Sleeve Gastrectomy</b></p><p><b>Jayamini Kaushalya</b>, Anjana Abayasinghe, Samitha Senevirathne and Ishan De Zoysa and Nilesh Fernandopulle</p><p><i>Professorial Surgical Unit, National Hospital, Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Obesity is a major healthcare burden. Laparoscopic sleeve gastrectomy (LSG) is a widely used bariatric procedure that achieves substantial weight loss and improves obesity-related comorbidities. Even though rare, leakages from the stapler line occur in 1–3% of patients, posing a severe complication. Such leaks can lead to serious, life-threatening consequences if not managed promptly and effectively.</p><p><b><i>Case report:</i></b> A 58-year-old female with grade 2 obesity, complicated by metabolic syndrome, obstructive sleep apnea, and bilateral knee osteoarthritis, underwent LSG. Post-operatively, on day 15, she was diagnosed with a leakage from the stapler line at the gastric fundic region. Initial management involved endoscopic clipping of the defects and placement of a gastric mega stent. Following the failure of those interventions, subsequently, endoscopic application of two ASD (Atrial Septal Defect) closure devices was employed to manage the leak. This approach proved successful, effectively sealing the defect and allowing for resolution of the leakage.</p><p><b><i>Discussion:</i></b> Management of leaks following LSG requires a tailored approach based on the patient's hemodynamic stability and the extent of the leak. Initial management strategies involve patient resuscitation, followed by endoscopic evaluation and interventions such as stent placement, clipping, or endoluminal wound vacuum. This case demonstrates the potential of repurposing ASD closure devices in managing post-LSG leaks. While the literature on this application is limited, it offers a promising alternative in the failure of conventional treatments. Further, this highlights the importance of innovative approaches to complex surgical complications.</p><p><b>PP-01-140</b></p><p><b>Endoscopic resection for low grade duodenal neuroendocrine tumors: A single center-retrospective analysis</b></p><p>Jin Ook Jang, Cheol Woong Choi, Eun Jeung Choi, Su Bin Sin and Cheol Min Lee and <b>Woojin Kim</b></p><p><i>Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan-si, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> High resolution videoendoscopic system and increased number of screening endoscopic examination enable us to detect incidental small duodenal neuroendocrine tumor (D-NET). The selection between radical surgical resection and local excision, including endoscopic resection for D-NET remains controversial. Recent guidelines recommend endoscopic resection for D-NET < 10 mm. Considering recent advancements in therapeutic endoscopic procedures, we aimed to evaluate the feasibility of diagnostic endoscopic resection of D-NETs grade 1 or 2.</p><p><b><i>Methods:</i></b> We retrospectively analyzed forty D-NETs diagnosed with grade 1 or 2 at a single tertiary referral center between January 2009 and July 2023.</p><p><b><i>Results:</i></b> The mean follow-up period was 59.0 ± 6.8 months. The mean NET size was 7.4 ± 0.8 mm, and most patients’ NETs were <10 mm (80.0%). The maximal NET diameter of NETs was 25 mm. During the study period, most NETs were grade 1 (n=36, 90%). Only 17.5% of patients were treated surgically at first. After endoscopic treatment, 2 additional surgical resection were performed, which revealed no evidence of lymph node metastasis. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Among endoscopic resections, ligation assisted endoscopic mucosal resection (EMR) showed a 100% complete resection rate.</p><p><b><i>Conclusions:</i></b> We observed that small-sized D-NETs grade 1 and 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection could be recommended for D-SETs with grade 1 or 2. Ligation assisted EMR is preferred for less than 10 mm in size.</p><p><b>PP-01-141</b></p><p><b>The endoscopic resection for low grade gastric neuroendocrine tumors: A retrospective analysis</b></p><p>Won Jun Jang, Cheol Woong Choi, Eun Jeung Choi, <b>Woojin Kim</b> and Su Bin Sin</p><p><i>Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan-si, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The recent surge in screening endoscopy has led to increased detection of gastric neuroendocrine tumors (G-NETs). The selection between radical surgical resection and local excision, including endoscopic resection, for the management of G-NETs remains controversial. Currently, endoscopic resection is recommended for G-NETs < 10 mm. We aimed to evaluate the feasibility of diagnostic endoscopic resection of G-NETs.</p><p><b><i>Methods:</i></b> We retrospectively analyzed 31 patients diagnosed with grade 1 or 2 G-NETs at a single tertiary referral center between January 2009 and December 2023. Outcomes, including histopathology, complete resection, and metastasis rates, were analyzed.</p><p><b><i>Results:</i></b> The mean follow-up period was 38.9 ± 38.4 months. The mean size of G-NET was 4.9 ± 3.4 mm, and most patients’ NETs were <10 mm (87.1%). The maximal NET diameter of NETs was 16 mm. During the study period, most NETs were grade 1 (type 1: 90.9%; type 3: 85.0%). R1 resection patients (19.4%) showed no evidence of metastasis during follow-up without additional surgical management. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Recurrent or multiple G-NETs were observed only in the patients with type 1 NETs (27.2%, 3/11). Modified endoscopic mucosal resection (EMR) (precut, ligation assisted, cap-assisted, and underwater), and endoscopic submucosal dissection (ESD) showed a 100% complete resection rate.</p><p><b><i>Conclusions:</i></b> We observed that G-NETs 1 or 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection is recommended for gastric SETs ≤16 mm in size. Modified EMR or ESD is preferred to conventional EMR.</p><p><b>PP-01-142</b></p><p><b>Characteristics and predictive scoring system for hemorrhagic duodenal ulcer with difficult endoscopic hemostasis</b></p><p><b>Marie Kurebayashi</b>, Akira Hashimoto, Shoma Sawai, Takahiro Ono, Yuichi Tahara, Naoki Kuroda, Masatoshi Aoki, Hiroyuki Fuke, Hiroyuki Kawabata, Yoshihiro Wakita and Atsuya Shimizu</p><p><i>Saiseikai Matsusaka General Hospital, Matsusaka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To clarify the characteristics of hemorrhagic duodenal ulcer (HDU) with difficult endoscopic hemostasis and whether existing scoring systems can be used to predict difficult-to-treat cases.</p><p><b><i>Materials and Methods:</i></b> Between January 2008 to June 2024, we retrospectively enrolled 91 patients. HDU was defined as ulcer that required some kind of hemostatic treatments. <Study1> We classified the patients into two groups, group A (GA), difficult endoscopic hemostasis) and group B (GB), successful hemostasis, and compared the patient backgrounds, ulcer locations and findings. <Study2> We evaluated which scoring systems for upper gastrointestinal bleeding (Glasgow-Blatchford Score (GBS), AIMS65 score, clinical Rockall score (CRS)) can predict difficult endoscopic hemostasis using the area under the ROC curve (AUC).</p><p><b><i>Results:</i></b> <Study1> GA had 12 cases, and GB had 79 cases. History of duodenal ulcer was more common in GA than in GB (55 vs 19%, p=0.01). There were no differences in age, gender, NSAIDs/antithrombotic medication or ulcer locations. At the time of bleeding, GA had significantly lower systolic blood pressure compared with GB(93 vs 110mmHg, p=0.04), lower hemoglobin(6.0 vs 8.2 g/dl, p=0.03), lower albumin(2.5 vs 3.3 g/dl, p=0.02)and prolonged PT-INR(1.39 vs 1.13, p=0.007). <Study2> AUC [95%CI]: GBS 0.75[0.58-0.90]/AIMS65 0.80[0.64-0.95]/CRS 0.73[0.60-0.85]. AIMS65 was most useful, and the cutoff value for endoscopic hemostatic difficulty was score 3. All cases with AIMS65 score 0 had endoscopic hemostasis.</p><p><b><i>Conclusion:</i></b> HDU with hypotension, severe anemia, hypoalbuminemia, and prolonged PT-INR had difficulty with endoscopic hemostasis. AIMS65 was useful in predicting HDU with difficult endoscopic hemostasis.</p><p><b>PP-01-143</b></p><p><b>Comparison of EUS-FNB and Artificial Intelligence-based EUS Imaging in the Diagnosis of Subepithelial Lesions <20mm</b></p><p><b>Yosuke Minoda</b>, Shuzaburo Nagatomo, Yusuke Kimura, Haruei Ogino and Eikichi Ihara</p><p><i>Kyushu University Hospital, Fukuoka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Although Endoscopic ultrasound fine-needle biopsy (EUS-FNB) is the gold standard for diagnosing gastric subepithelial lesions (SELs), its accuracy is low for SELs <20mm. With advancements in endoscopic techniques allowing for full-thickness resection of SELs, accurate and simple diagnosis of small SELs could offer significant healthcare benefits. Therefore, leveraging rapidly developing artificial intelligence (AI) technology, we created an AI system for gastric SELs (referred to as EUS-AI) and compared its diagnostic accuracy with EUS-FNB for SELs < 20mm.</p><p><b><i>Materials and Methods:</i></b> From April 2010 to March 2020, we evaluated the diagnostic accuracy of EUS-AI using 250 cases of gastric SELs assessed by EUS and pathologically confirmed via EUS-FNAB/surgical intervention. We trained the AI using a deep learning system with neural networks on EUS images of SELs. For validation, we trimmed EUS images of 30 consecutive cases with gastric SELs under 20mm to include only the lesion areas and had AI diagnose these, comparing it to EUS-FNB. Diagnostic capabilities were measured by accuracy, sensitivity, and specificity for GIST/non-GIST.</p><p><b><i>Results:</i></b> EUS-FNB had a tissue acquisition rate of 66.7% for gastric SELs under 20mm, with an accuracy of 66.7%, sensitivity of 66.7%, and specificity of 100%. EUS-AI achieved an accuracy of 76.7%, sensitivity of 78.6%, and specificity of 75%. There were 11 cases where EUS-FNB could not obtain tissue, and EUS-AI correctly diagnosed 82% of these.</p><p><b><i>Conclusion:</i></b> EUS-AI demonstrated comparable diagnostic accuracy to EUS-FNB for lesions under 20mm and could potentially aid future clinical practices.</p><p><b>PP-01-144</b></p><p><b>Impact of intra-tumoral injection of Phosphorus-32 microparticles on vascularity in locally advanced pancreatic carcinoma</b></p><p>Amanda Lim<sup>1,2</sup>, Joshua Zobel<sup>1</sup>, Madison Bills<sup>4</sup>, William Hsieh<sup>4</sup>, Benjamin Crouch<sup>4</sup>, Rohit Joshi<sup>5</sup>, John-Edwin Thomson<sup>6</sup>, Eu Ling Neo<sup>6</sup>, Li Lian Kuan<sup>6</sup>, Ms Romina Safaeian<sup>1</sup>, Edmund Tse<sup>1,2</sup>, Christopher Rayner<sup>1,2</sup>, Andrew Ruszkiewicz<sup>2,8,9</sup>, Singhal Nimit<sup>3</sup>, Dylan Bartholomeusz<sup>1,4</sup> and <b>Nam Nguyen</b><sup>1,2</sup></p><p><sup>1</sup><i>Gastroenterology and Hepatology, Royal Adelaide Hospital, Adelaide, Australia;</i> <sup>2</sup><i>The University of Adelaide, Adelaide, Australia;</i> <sup>3</sup><i>Department of Oncology, Royal Adelaide Hospital, Adelaide, Australia;</i> <sup>4</sup><i>Department of Nuclear Medicine, Royal Adelaide Hospital, Adelaide, Australia;</i> <sup>5</sup><i>Department of Oncology, Lyell McEwin Hospital, Adelaide, Australia;</i> <sup>6</sup><i>Department of Hepatobiliary Surgery, Royal Adelaide Hospital, Adelaide, Australia;</i> <sup>7</sup><i>Department of Upper Gastrointestinal Surgery, Queen Elizabeth Hospital, Adelaide, Australia;</i> <sup>8</sup><i>Surgical Pathology, SA Pathology, Adelaide, Australia;</i> <sup>9</sup><i>Centre of Cancer Biology, University of South Australia, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Dense stroma and poor vascularity are potential causes for chemo-resistance in pancreatic cancer. Gemcitabine/Nab-Paclitaxel chemotherapy has no impact on vascularity. This study aimed to assess vascularity changes within pancreatic tumours following implantation of 32P microparticles (OncoSil) in addition to standard chemotherapy.</p><p><b><i>Materials and Methods:</i></b> Clinico-pathological outcomes and contrast-enhanced harmonic EUS (CH-EUS) was prospectively assessed in locally advanced pancreatic cancer (LAPC) patients who received OncoSil and FOLFIRINOX chemotherapy. CH-EUS were performed before, 4 weeks and 12 weeks after OncoSil implantation. Time intensity curve (TIC) and peak intensity analyses were markers of microvascular flow and reflect the vascularity of the mass lesion.</p><p><b><i>Results:</i></b> 20 patients were recruited, with 15 completing 12-week follow-up. OncoSil implantation was successful in all patients with no procedural complications. At 12 weeks, median tumour size was significantly reduced (32mm to 24mm; p<0.001), with 5 patients (25%) had tumour downstaging for resections. There was significant intensity gain of contrast enhancement within the tumour after 4 and 12 weeks of OncoSil-implantation (baseline = 32.15 (IQR 18.08-54.35) vs.46.85 (IQR 35.05-76.6; p=0.007) vs. 66.3 (IQR 54.7-76.3; p=0.001, respectively). Estimated median survival was 16.0 months, with 80% of patients still alive and only 3/20 (15%) demonstrating both local- and distant-disease progression.</p><p><b><i>Conclusion:</i></b> OncoSil implantation in addition to chemotherapy is not only associated with promising clinic-pathological outcomes, but also increased vascularity within pancreatic cancer. These findings suggest that potential impact of OncoSil on the tumour micro-environment and allow more delivery of chemotherapy agents to the cancer. A larger comparative trial is warranted.</p><p><b>PP-01-145</b></p><p><b>Endoscopic removal of foreign bodies from the gastrointestinal tract in children</b></p><p><b>Stanislaw Pieczarkowski</b>, Kinga Kowalska-Duplaga, Malgorzata Sladek, Zofia Grzenda-Adamek, Bartosz Bogusz, Dariusz Chmiel and Krzysztof Fyderek</p><p><i>University Childrens Hospital Jagiellonian University Faculty of Medicine, Krakow, Poland</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Aim:</i></b> Assessment of factors that may affect the success of the procedure.</p><p><b><i>Material and methods:</i></b> Between 2012 and 2024, Ingested foreign bodies (IFB) was diagnosed in 171 children, 87 boys and 84 girls aged 0.4 to 16 years.</p><p>The patients were divided into two groups: Group 1 (128patients) - in which the removal of IFB with the first tool used was effective and group 2 (43 patients) - in which it was NOT effective. Both groups were analyzed in relation to the location of the IFB, the type of IFB and the tool used.</p><p><b><i>Results:</i></b> The most frequently removed IFB were coins (n = 84; including 68 in group 1 and 16 in group 2) and button batteries (n = 19, including 15 in group 1 and 4 in group 2). There were no statistically significant differences between the study groups in terms of children's age, gender, time since IFB, or endoscopist. The effectiveness of the first tool used was 100% for magnets, 80.95% for coins, 79% for button batteries. The following were also analyzed: dependence between time from IFB and the number of tools used (p=0.8514), dependence between location of IFB and the number of tools used, (p=0.0541).</p><p><b><i>Conclusions:</i></b> For coins, rat teeth are the most effective tool, while for button batteries and other types of IFBs, a Roth net is more useful. The problem is the type of IFB and its location, not the instrument used, the age, gender of the child or the endoscopist performing the procedure</p><p><b>PP-01-146</b></p><p><b>Differences in monocyte to lymphocyte ratio levels in gastritis and peptic ulcer cases</b></p><p><b>Tatag Primiawan</b></p><p><i>Gadjah Mada University, Magelang, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Peptic ulcers are abnormalities that are often found on endoscopic examination. The monocyte lymphocyte ratio examination is a simple examination that can be used to determine the prognosis of upper gastrointestinal disorders.</p><p>The aim of this study was to compare Monocyte-to-lymphocyte ratio (MLR) between gastritis patients and peptic ulcer patients.</p><p><b><i>Method:</i></b> We carried out a retrospective analysis of patients who underwent endoscopy from July 2023 to January 2024. Demographic data, endoscopy results, laboratory parameters were taken from medical records. The monocyte to lymphocyte ratio was analyzed using statistical analysis.</p><p><b><i>Result:</i></b> Based on endoscopy results, a total of 94 patients were included in this study, with 69 (73.4%) diagnosed with gastritis and 25 (26.6%) with peptic ulcers. The cohort comprised 40 (42.6%) males and 54 (57.4%) females.</p><p>The median MLR for gastritis patients was 0,3 (range 0,1 – 0,7), whereas for peptic ulcer patients, it was 0,28 (Range 0,13 – 0,79). A statistically significant difference in MLR was observed between patients with gastritis and those with peptic ulcers (p=0.022).</p><p>The ROC curve analysis identified a cut-off value of 0,37 forMLR. The odds ratio for the association between MLR and the occurrence of peptic ulcers is 8,14.</p><p><b><i>Conclusion:</i></b> Our study revealed a statistically significant difference in (MLR) between patients with gastritis and those with peptic ulcers. The MLR value Lower than 0,37 is a significant risk factor for peptic ulcer.But this difference was not at a level that could be used in clinical practice.</p><p><b>PP-01-147</b></p><p><b>Endoscopic Hemostasis Procedures in Non Variceal upper gastrointestinal Hemorrhage at Jakarta Tertiary hospital</b></p><p><b>Tri Ferry Rachmatullah</b> and Hasan Maulahela</p><p><i>Cipto Mangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Non variceal upper gastroinstestinal hemorrhage (NVUGIH) is a common gastrointestinal condition encountered in daily practice. The causes are gastroduodenal ulcers, vascular abnormalities, tumours, iatrogenic and Mallory Weiss syndrome. Several hemostasis endoscopic methods are being used to treat the hemorrhage.</p><p><b><i>Objectives:</i></b> To find out causes and hemostasis procedures in NVUGIH patient at digestive endoscopy department Cipto Mangunkusumo hospital Jakarta.</p><p><b><i>Materials and Methods:</i></b> This is a descriptive cross sectional study from medical record data that include 79 NVUGIH patient underwent endoscopic hemostasis procedure at digestive endoscopy department from January 2020 to June 2024.</p><p><b><i>Results:</i></b> Endoscopic hemostasis were most performed in male patient (77,2%) at aged range of 41 to 60 years (39,2%). Melena is the most common indication (64,5%). The hemorrhage were caused by peptic ulcer disease (37,9%), gastroduodenal tumours (29,1%), iatrogenic (29,1%), arteriovenous malformation (2,5%) and foreign body (1,26%). Adrenaline injection (AI) combine with hypertonic dextrose spray (HDS) is the most common hemostasis procedure (34,1%). The others are HDS (20,2%), hemoclip (12,6%), Argon plasma coagulation (APC) with HDS (5,06%), hemoclip with AI (3,79%), hemoclip with HDS (3,79%), APC with AI and HDS (3,79%), APC (2,5%), APC with AI (2,5%), hemoclip with APC and AI (1,26%), hemoclip with APC (1,26%), hemostatic forcep with HDS (1,26%) and hemostatic forcep with APC, AI and HDS (1,26%).</p><p><b><i>Conclusion:</i></b> Peptic ulcer disease and combination between adrenaline injection with hypertonic dextrose spray is the most common cause and hemostasis procedure on NVUGIH patient at digestive endoscopy department Cipto Mangunkusumo hospital Jakarta.</p><p><b>PP-01-148</b></p><p><b>Transnasal versus conventional gastroscopy: comparison of tolerability and diagnostic outcome</b></p><p><b>Kimchhay Ro</b><sup>1</sup>, Chakravuth Oung<sup>2</sup> and Sereyvathana Chhut<sup>2</sup></p><p><sup>1</sup><i>Calmette Hospital, Phnom Penh, Cambodia;</i> <sup>2</sup><i>University of health sciences, Cambodia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The main objective of this study was to assess tolerability and diagnostic outcome of transnasal esophagogastroduodenoscopy (T-EGD) in comparison with conventional esophagogastroduodenoscopy (C-EGD).</p><p><b><i>Materials and methods:</i></b> This was a retrospective, descriptive, analytical, monocenter study and was conducted from 2010 to 2017 which included 3,637 patients over the age of 18 years old who underwent endoscopy without sedation using 5.5 mm and 9.2 mm endoscope. Patient data were noted regarding tolerability, acceptance and endoscopy performance using visual analogue scale (VAS) and diagnostic outcome.</p><p><b><i>Result:</i></b> In 3637 patients underwent unsedated gastroscopy (T-EGD 1738, C-EGD 1899), benign and malignant findings are comparable in both group. VAS scores for patient tolerability were significantly better in the T-EGD group (7.09 vs 6.07, P < 0.001). T-EGD was more comfortable and better tolerated than previous endoscopy in 87% of C-EGD experienced patient (34 out of 39). 89.8% of T-EGD group preferred the same procedure for their future endoscopy, compare to 70.9% in C-EGD group.</p><p><b><i>Conclusion:</i></b> Transnasal endoscopy is superior to C-EGD in terms of comfort and patient acceptance while maintaining an efficient diagnosis and acceptable performance. T-EGD is suitable as an alternative endoscopy exam for patients who are at high risk of sedation-related complications and can't tolerate conventional gastroscopy.</p><p><b>PP-01-149</b></p><p><b>The Evolution of Double Pylorus Associated With NSAIDs-Induced Gastric Ulcer</b></p><p><b>Rasco Sandy Sihombing</b><sup>1</sup>, Taolin Agustinus<sup>1</sup> and Ari Fahrial Syam<sup>2</sup></p><p><sup>1</sup><i>RSUD Mgr Gabriel Manek, SVD, Atambua, Atambua, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia / Cipto Mangunkusumo General National Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Double pylorus (DP) is an uncommon condition when two communicating channels between the gastric antrum and duodenal bulb exists. According to the etiology, DP can be categorized as acquired and congenital. Acquired DP is usually preceded by gastric ulcer.</p><p><b><i>Case Report:</i></b> A 77-year old male patient with the history of frequent using of non steroidal anti-inflammatory drugs (NSAIDs) was admitted because of hematemesis melena. The esofagogastroduodenoscopy (EGD) showed the two openings separated by bridging tissue at the end of the antrum. Both openings were led the scope to the duodenal bulb. There was also an ulcer at small curvature side of the antrum. Nine months later, EGD showed one large opening connecting the antrum and duodenal bulb suggested as fusion between the two channels.</p><p><b><i>Discussion:</i></b> The prevalence of DP is reported about 0,001-0,4%. The acquired DP is possibly formed by the advanced erosion between gastric and duodenal ulcer. Another mechanism is the gastric scarring which attracts the lesser curvature and later perforated into the duodenal bulb. The congenital type of DP is less often and usually occurs in young patients with other gastrointestinal congenital abnormalities. The single large opening in the third endoscopy is the fusion between the two channels caused by septal destruction that may be related to fistulous tract ulceration or mechanical damage by food bolus. The treatment of acquired DP is just focused on factors that influence the gastric ulcer like stopping the NSAIDs and eradicating the H. pylori. Surgery is extremely rare to perform.</p><p><b>PP-01-150</b></p><p><b>Massive obscure lower gastrointestinal bleeding from predominant ectopic pancreatic tissue in a Meckel’s diverticulum</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Ashan Jayawickrama<sup>2</sup>, Omar Ozaal<sup>2</sup>, Gayani Ranaweera<sup>3</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, University of Colombo, Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Obscure gastrointestinal bleeding(OGIB) represents about 5% of all gastrointestinal haemorrhages which is characterized by continuous or recurrent bleeding from an undetermined source after an initial negative bidirectional endoscopy. Meckel's diverticulum(MD) is a rare but recognized cause of OGIB which usually contain ectopic gastric mucosa. Here we present a patient with MD contained predominant ectopic pancreatic tissue resulted in massive OGIB.</p><p><b><i>Case Description:</i></b> A 35-year-old female with recurrent rectal bleeding over 7 years and severe anemia underwent multiple inconclusive investigations. Previous episodes were resolved with blood transfusions. On admission, she was pale, tachycardic, and hypotensive. Initial tests, including a CT angiogram, bidirectional gastrointestinal endoscopies and a small bowel capsule endoscopy, did not provide a clear cause. She experienced massive bleeding, requiring emergency laparotomy which revealed a MD with thickened mucosa. An improvised intraoperative endoscopy via appendix confirmed no colon bleeding, leading to resection of the diverticulum. Her post-operative period was uneventful with stable haemoglobin levels. Histology showed predominant ectopic pancreatic mucosa with a focus of gastric tissue in the resected MD.</p><p><b><i>Discussion:</i></b> MD and its complications, especially gastrointestinal bleeding, are more common in children than in adults. This case discusses the rarity of histological presentation of MD with predominant pancreatic tissue leading to massive OGIB. It also discusses the importance of intraoperative decision-making and intra operative enteroscopy in cases of OGIB when other tests are negative before surgical resection of symptomatic MD.</p><p><b>PP-01-151</b></p><p><b>A UK hospital experience on feasibility of transnasal endoscopy (TNE) service in outpatients setting</b></p><p><b>Cheh Kuan Tai</b></p><p><i>Royal Free NHS Trust, London, London, United Kingdom</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>During the height of the COVID-19 pandemic in 2020, elective endoscopy services within the UK National Health Service came to a standstill for 4 months. With subsequent waves, the redeployment of endoscopy staff further compounded the endoscopy backlog, with surveillance procedures being impacted the most.</p><p>In the UK, endoscopy services are delivered in dedicated endoscopy units. Gastroscopies are generally performed with local anaesthetic with or without sedation. Each endoscopic list requires 2 assistants-one trained nurse and one health care assistant.</p><p>In 2022, our gastric ulcer rescope audit showed that 70% of patients did not receive their repeat procedure within 12 weeks. We developed a transnasal endoscopy (TNE) service to increase capacity. TNE cause less haemodynamic instability. It is generally better tolerated and obviate the need for sedation. The nursing provision could also be reduced to 1 trained nurse.</p><p>We began a pilot TNE service in October 2023 in the outpatient clinic setting. In the 9 months between October and June 2023, we have delivered 31 TNE lists and 172 cases. The numbers of cases completed per list was initially low at 4 to allow for training of staff. There has not been any complications encountered thus far. 4% of patients did not tolerated the procedure unsedated and had to be rebooked.</p><p>This service has allowed us to clear the surveillance backlog for gastric ulcer and oesophagitis rescopes and we are currently performing prospective diagnostic requests. This pilot demonstrates the feasibility of a TNE service based in outpatients.</p><p><b>PP-01-152</b></p><p><b>Sloughing Esophagitis In A 84-Year-Old Patient: A Case Report</b></p><p><b>Pham Phuong Thu Tran</b> and Ngoc Luu Phuong Tran</p><p><i>Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Viet Nam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Sloughing esophagitis is characterized by sloughing of large vertical fragments on the superficial esophageal mucosa without inflammation. Due to the absence of specific features indicative of esophageal disorders, this disease has a very low incidence and is often unexpectedly diagnosed by esophagogastroduodenoscopy (EGD). Risk factors involve medications, toxic chemicals, or immune system disorders, but the primary cause remains unknown. In this case report, we describe the patient’s characteristics and compare them with the previous case reports to find out appropriate diagnosis and treatment approach for subsequent cases.</p><p><b><i>Case description:</i></b> A 84-years-old man was admitted to hospital with chief complain of two-day melena and a notable symptom was hiccups for two weeks leading to malabsorption. His medical history included hypertension and atopic dermatitis, and unremarkable signs at diagnosis. Therefore, urgent EGD was performed, revealing a peptic ulcer with the Forrest type III and suspected sloughing esophagitis. Further examination reminded him that he had been experiencing heartburn, retrosternal pain, dysphasia with solid and liquid food for the past month, but these symptoms had been not noteworthy. Finally, he was partially recovered by intravenous proton pump inhibitor (PPI) after 10-day treatment.</p><p><b><i>Discussion:</i></b> Sloughing esophagitis occurring in the middle – distal esophagus is a benign and self-limited illness. In cases of severe erosion reflux disease or nonvariceal upper gastrointestinal bleeding, PPI has shown efficacy. However, refractory esophagitis may require additional treatment with intravenous glucocorticoids. Given the nonspecific symptoms, patients with sloughing esophagitis should be re-evaluated by repeat EGD to monitor recovery and complications.</p><p><b>PP-01-153</b></p><p><b>Long-term PPI users should avoid alcohol consumption and receive endoscopy follow up regularly</b></p><p><b>Yu-Ching Tsai</b><sup>1,2</sup>, Hsiao-Bai YANG<sup>3,5</sup>, Ming-Tsung HSIEH<sup>2</sup>, Chun-Te LEE<sup>2</sup>, LO Yi-Ning<sup>1,2</sup>, Hsiu-Chi CHENG<sup>2,4,6</sup> and Bor-Shyang SHEU<sup>2,4</sup></p><p><sup>1</sup><i>Tainan hospital ministry of health and welfare, Tainan, Taiwan;</i> <sup>2</sup><i>Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;</i> <sup>3</sup><i>Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;</i> <sup>4</sup><i>Institute of Clinical Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;</i> <sup>5</sup><i>Department of Pathology, Ton Yen General Hospital, Hsin-Chu, Taiwan;</i> <sup>6</sup><i>Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to identify risk factors associated with the presence/progression of pre-cancerous lesions such as advanced gastric atrophy/intestinal metaplasia (OLGA/OLGIM stage III/IV) or corpus-predominant gastritis index (CGI) and whether endoscopic features of precancerous lesions were associated with pathological diagnosis among long-term PPI users.</p><p><b><i>Materials and methods:</i></b> 73 long-term PPI users were enrolled to receive gastroscopy and topographic biopsies and assess the presence of OLGA/OLGIM stage III/IV and CGI. With comparisons with their previous endoscopic studies before PPI use, changes of OLGA/OLGIM and CGI were compared to risk factors and NBI endoscopy images.</p><p><b><i>Results:</i></b> We found consistencies between the previous and current high-risk IM features (OLGIM III/IV+corpus IM) (P<0.05). Alcohol consumption was associated with the presence of CGI (P=0.002). Under NBI endoscopy, the Kimura-Takemoto classification, EGGIM, and corpus mucosa with groove pattern/dilated sub-epithelial capillaries were associated with OLGA stage III/IV (P=0.048), OLGIM stage III/IV (P<0.001), high corpus inflammation (P<0.001), and CGI (P=0.05). As compared with previous biopsies, the progression rates of OLGA III/IV, OLGIM III/IV/corpus IM, and CGI were 20.3%, 3.4% and 10.5%, respectively. The progression/regression of IM and CGI based on pathology was moderately associated with the progression/regression based on endoscopic images, including EGGIM (Pearson’s R=0.609, P<0.001) and corpus inflammation (Pearson’s R=0.396, P=0.003) after long-term PPI use. Alcohol drinking was associated with the progression of precancerous lesions (P=0.023).</p><p><b><i>Conclusion:</i></b> Alcohol was a risk factor of CGI and the progression of precancerous lesions among long-term PPI users. Endoscopic features after long-term PPI use were correlated with pathological diagnosis.</p><p><b>PP-01-154</b></p><p><b>Gastric epithelial neoplasm of fundic-gland mucosa lineage</b></p><p><b>Hiroya Ueyama</b> and Takashi Yao and Yoichi Akazawa and Takuo Hayashi and Tsutomu Taeda and Kumiko Ueda and Mariko Hojo and Akihito Nagahara</p><p><i>Department Of Gastroenterology, Juntendo University School Of Medicine, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We previously proposed a histopathological classification of gastric epithelial neoplasm of fundic-gland mucosa lineage (GEN-FGML) [1,2]. However, the clinicopathological features in a large number of cases have not been well investigated. The aim of this study is to clarify the clinicopathological features of GEN-FGML according to the histopathological classification.</p><p><b><i>Materials and Methods:</i></b> We performed a retrospective study to investigate the clinicopathological and endoscopic features of GEN-FGML. GEN-FGML was classified into 3 major types, oxyntic gland adenoma (OGA), gastric adenocarcinoma of fundic-gland type (GA-FG), and gastric adenocarcinoma of fundic-gland mucosa type (GA-FGM).</p><p><b><i>Results:</i></b> A total of 150 GEN-FGML lesions in 113 patients were retrospectively collected between July 2008 and March 2024. The average of tumor size was OGA 3.8mm, GA-FG 7.9mm, and GA-FGM 9.5mm. The depth of submucosal invasion (μm, GA-FG 275.7, GA-FGM 555.9, p<0.05) were significantly greater and the rates of lymphatic invasion (GA-FG 1/70, GA-FGM 3/26, p<0.05) were significantly higher in GA-FGM than in GA-FG. The rate of non-curative endoscopic resection was significantly higher in GA-FGM than in GA-FG (GA-FG 8/69, GA-FGM 7/15, p<0.01). OGA and GA-FG demonstrated low-grade epithelial neoplasm, and GA-FGM should be categorized as an aggressive variant of GEN-FGML that demonstrated high-grade epithelial neoplasm as reported previously.</p><p><b><i>Conclusion:</i></b> This classification is useful to estimate its malignant potential and establish a standard therapeutic approach for GEN-FGML.</p><p><b>References:</b></p><p>\n [1] <span>Ueyama, H</span> et al. <i>J Gastroenterol.</i> <span>2021</span>. <span>56</span>(<span>9</span>): <span>814</span>-<span>828</span>.</p><p>\n [2] <span>Ueyama, H</span> et al. <i>Am J Surg Pathol.</i> <span>2010</span>. <span>34</span>(<span>5</span>): <span>609</span>-<span>19</span>.</p><p><b>PP-01-155</b></p><p><b>A Novel Suturing Technique for the Endoscopic Full-Thickness Resection Defects Using a Long Transparent Cap</b></p><p><b>Saif Ullah</b>, Nan Dai and Xinguang Cao</p><p><i>The First Affiliated Hospital Of Zhengzhou University, Zhengzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To evaluate the safety and efficacy of a novel long transparent cap suturing technique for the closure of large endoscopic full-thickness resection (EFTR) defects.</p><p><b><i>Methods:</i></b> A total of 62 patients who underwent EFTR for their large gastric submucosal tumors at the First Affiliated Hospital of Zhengzhou University between January 2017 and March 2023 were included in this study. All the EFTR defects was closed using the long transparent cap suturing technique (Graphical abstract). The procedure success rate, tumor size, defect closure time, length of postoperative hospital stay, complications and recurrence rate were recorded and analyzed.</p><p><b><i>Results:</i></b> The procedure technical success rate was 100%. The median tumor size was 1.5 cm (range: 0.6-6.5 cm), and the median number of metal clips used was 7 (range: 4-14). The median EFTR procedure time was 53.5 minutes (range: 22-136 minutes), and the median suturing time was 14 minutes (range: 3-45 minutes). The median length of postoperative hospital stay was 3.5 days (range: 3-6 days). Postoperative complications including bleeding occurred in 1 patient, abdominal pain in 2 patients, and fever observed in three patients. During the median 29 months (range: 7-79 months) of follow up, no residual lesions, tumor recurrence, or metastasis were detected, and no formation of digestive tract fistulas or sinuses was observed.</p><p><b><i>Conclusion:</i></b> The long transparent cap suturing technique is a simple, safe, and effective method for large defects closure after EFTR. Its advantages includes, affordability, simplicity, and widespread availability, making it suitable for widespread clinical application.</p><p><b>PP-01-156</b></p><p><b>The smart approach to surgical treatment for gastric and duodenal gists based on preoperative eus-typing</b></p><p><b>Seda Dzhantukhanova</b> and Yury Starkov and Ayubkhan Vagapov and Rodion Zamolodchikov and Amina Badakhova</p><p><i>Vishnevsky National Medical Research Center for Surgery, Moscow, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To develop and demonstrate different surgical techniques of laparoscopic or endoscopic resection for GISTs based on classification of EUS-typing for optimal choice of treatment</p><p><b><i>Materials and Methods:</i></b> The EUS-classification of GISTs was created based on the analysis of treatment of 80 patients with gastric and duodenal GISTs. The principles of classification included the following criteria: location of tumor base in relation to GI layer Size of the tumor base (“growing point”) Type of growth in relation to GI lumen The EUS-typing includes Type I, Type II, Type III (a, b, c, d) tumors (table)</p><p><b><i>Results:</i></b> Patients with gastric and duodenal GISTs underwent laparoscopic resection – 62, endoscopic intraluminal resections – 18 patients (tunneling resection – 7, endoscopic submucosal dissection or endoscopic enucleation of tumor after resection of covering mucosa – 11) Median operation time was 150 min. Recovery was uneventful and median post-op hospital stay was 5 ± 2,4 (2-8) days. The pathology showed R0 resection in all cases. Histology and immunohistochemistry confirmed GIST. Four patients received adjuvant target therapy and were prescribed Gleevec for 1-2 years. The mean follow-up period was 42 months (range 3-74 months) with no local or distant recurrence or stenosis at the site of surgery</p><p><b><i>Conclusion:</i></b> The classification of GISTs based on EUS-typing allows to select the optimal approach individually for each patient to perform surgery more accurate and less invasive</p><p><b>PP-01-157</b></p><p><b>EXPLORE AI-BO Study: Exploring Endoscopists’ Knowledge and Perception Towards Artificial-Intelligence in Surveillance for Barrett’s Oesophagus</b></p><p><b>Nicholas Wan</b><sup>1,2</sup>, Jin Tan<sup>1,2</sup>, Celine Chan<sup>1</sup>, Asif Chinnaratha<sup>1,2</sup> and Rajvinder Singh<sup>1,2</sup></p><p><sup>1</sup><i>Gastroenterology Department, Lyell McEwin Hospital, Elizabeth Vale, Australia;</i> <sup>2</sup><i>Adelaide Medical School, University of Adelaide, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Artificial Intelligence (AI) has been shown to assist endoscopists in detecting early neoplasia in Barrett’s Oesophagus (BO). We aim to explore endoscopists’ knowledge and perception towards AI in BO, providing insights into factors impacting its clinical implementation.</p><p><b><i>Methods:</i></b> A questionnaire consisting of six domains was disseminated to 140 endoscopists across South Australia.</p><p><b><i>Results:</i></b> Twenty participants completed the questionnaire. 1) Knowledge and Perception Towards AI in Endoscopy: 15% reported having adequate knowledge of AI-application in BO, compared to colorectal-polyp-detection (60%). Majority (85%) were willing to use AI for BO-surveillance. 2) Knowledge and Perception Towards AI in BO-Surveillance: White-Light-Imaging (60%) and Narrow-Band-Imaging (55%) were modalities where respondents had some knowledge of AI in BO-surveillance. Participants perceived that AI will improve the detection rates of low-grade-dysplasia (90%), high-grade-dysplasia (95%) and early-oesophageal-cancer (85%). 3) Impact on Patients: Half anticipated that AI would reduce the frequency of endoscopic surveillance. 4) Impact on Endoscopists: 75% indicated that AI could extend procedural duration. While some suggested an increase in fatigue (25%), others believed it decreases fatigue (50%). 5) Impact on Clinical Practice: All respondents felt that AI would enhance their accuracy in dysplasia detection. 70% opted to perform oesophageal biopsies even if AI had >95%sensitivity for detecting early-Barrett’s-dysplasia. 6) Medico-legal Concerns: Without regulation, only 50% were open to using AI. Accountability of missed-lesions was felt to be the responsibility of endoscopists (70%) vs AI (5%).</p><p><b><i>Conclusion:</i></b> Endoscopists showed positive attitudes towards integrating AI in BO-surveillance. Potential concerns include liability, procedural duration, and lack of regulation.</p><p><b>PP-01-158</b></p><p><b>Effect of GLP-1RA on residual gastric content during esophagogastroduodenoscopy:Systematic Review and Meta-Analysis of Cohort studies</b></p><p><b>Pamela Bianca Yap</b>, Mabel Angela Sarita and Sarah Jean Bellido</p><p><i>St.lukes medical center, Quezon city, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Glucagon-Like Peptide–1 Receptor Agonists(GLP-1 RA) have been used for the treatment of diabetes and management of obesity. Recent data shows increased incidence of endoscopically visible residual gastric contents (RGC) even with adequate fasting, predisposing sedated patients to adverse events. The American Society of Anaesthesiologists released a consensus guideline, holding GLP-1 RAs in the perioperative period though evidences were limited to several case reports. The objective of this study is to assess the effect of GLP-1 RA on RGC during upper endoscopy and risk for bronchopulmonary aspiration.</p><p><b><i>Materials and Methods:</i></b> A comprehensive search through different online data base was done which included studies determining the association of GLP-1 RA on RGC and bronchopulmonary risk during upper endoscopy. The quality of included studies was assessed using the Newcastle-Ottawa Scale(NOS) and GRADE Pro. Data was analyzed using Revman5.4. The primary outcome was presence of RGC and the effect measure used was odds ratio with confidence interval of 95%. Secondary outcome was bronchopulmonary risk</p><p><b><i>Results:</i></b> The search yielded 209 articles and 4 studies were included in this review. The studies included were assessed to have low risk of bias. Pooled results from the 4 studies showed increased RGC with GLP-1 RA use (OR 6.36, CI 5.20,7.78, p-value <0.00001). Bronchopulmonary risk increased with GLP RA use(OR 6.57, CI 1.35, 31.98, p-value <0.02). The studies, however, had very low to low certainty of evidence due to issues on study design and inconsistency.</p><p><b><i>Conclusion:</i></b> GLP-1 RA was associated with increased RGC in patients undergoing upper endoscopy.</p><p><b>PP-01-159</b></p><p><b>High-magnification NBI is an accurate tool for diagnosis of gastric neoplasia in a western population</b></p><p><b>Edward Young</b><sup>1,2</sup>, Nicholas Wan<sup>1</sup>, Andrew Ruszkiewicz<sup>1,3</sup> and Rajvinder Singh<sup>1,2</sup></p><p><sup>1</sup><i>Lyell McEwin Hospital, Northern Adelaide Local Health Network, Adelaide, Australia;</i> <sup>2</sup><i>Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia;</i> <sup>3</sup><i>SA Pathology, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In high-incidence eastern countries, nationwide gastric cancer screening programs have reduced the incidence of advanced gastric cancer by facilitating detection at an early stage amenable to endoscopic resection. In these countries, high-magnification narrow band imaging (M-NBI) has been established as an effective advanced mucosal imaging technique for gastric lesions. We aimed to assess the accuracy of this technique in a western population where data is limited.</p><p><b><i>Materials and Methods:</i></b> This is an analysis of a prospectively collected database of gastric lesions that were assessed and either biopsied or resected by an interventional endoscopist at a single Australian Hospital between 2009 and 2023. All lesions were assessed using M-NBI and a histological prediction made at the time of reporting. This was then correlated with the final histology.</p><p><b><i>Results:</i></b> A total of 232 lesions in 183 patients were included in the final analysis, including 35 adenomas, 29 early gastric cancers, 6 invasive adenocarcinomas, 137 non-neoplastic lesions and 25 ‘other’ lesions. For differentiating neoplastic versus non-neoplastic lesions, M-NBI had a sensitivity of 97.9% (CI 92.6-99.7%) and specificity of 97.1% (CI 92.7-99.2%). M-NBI also had high specificity (97.1%, CI 92.7-99.2%) in identifying lesions suitable for endoscopic resection, with a PPV of 96.9% (CI 89.2-99.6%). The observed agreement between the M-NBI predicted histology and the final pathological diagnosis was 91.81% with a derived kappa statistic of 0.865, indicating excellent agreement.</p><p><b><i>Conclusion:</i></b> M-NBI can be used with a high degree of accuracy in western populations, comparable to the accuracy demonstrated by experts in eastern countries.</p><p><b>PP-01-160</b></p><p><b>Risk Factors and Impact of Missed Adenomas in Colonoscopic Polypectomy with Different Endoscopic Systems</b></p><p><b>Chi Hyeon Choi</b> and Jong Yoon Lee</p><p><i>Dong-a University Hospital, Busan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to identify missed adenomas not detected in previous screenings or colonoscopies during polypectomy and to determine if the endoscopic system impacts the miss rate.</p><p><b><i>Materials and Methods:</i></b> This retrospective study includes patients who underwent colonoscopic polypectomy at Dong-A University Hospital from March 2020 to February 2022. Patients were referred after polyps were detected during screening or surveillance colonoscopies at other facilities. Colonoscopic polypectomies were performed by an expert using either the 'latest system' or the 'older system.' Missed polyps or adenomas were defined as those not documented in previous electronic photographs or endoscopic reports but detected during polypectomy. Miss rates were calculated by dividing the number of missed polyps/adenomas/advanced neoplasia by the total number of each among all patients. Advanced neoplasia was defined as any adenoma ≥ 10 mm, with villous histology, high-grade dysplasia, invasive cancer, or any SSP ≥ 10 mm or with dysplasia.</p><p><b><i>Results:</i></b> Out of 542 polyps, 186 were missed during initial screenings, resulting in a polyp miss rate of 25.55%. The miss rates for adenomas and advanced neoplasms were 27.34% and 14.69%, respectively. Univariate logistic regression analysis identified age, adenoma per colonoscopy, and endoscopic system as significant factors for adenoma detection. However, multivariate logistic regression analysis revealed that only the endoscopic system was statistically significant.</p><p><b><i>Conclusion:</i></b> The use of an older endoscopic system is significantly associated with missed adenomas detected by colonoscopic polypectomy.</p><p><b>PP-01-161</b></p><p><b>Factors predicting mortality in End Stage Renal Disease patients presenting with upper GI bleeding</b></p><p><b>Raja Taha Yaseen Khan</b> and Mahboob Jan and Abbas Ali Tasneem and Nasir Hassan Luck</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aims to identify factors that predict in-hospital mortality in ESRD patients presenting with non-variceal UGIB.</p><p><b><i>Methods:</i></b> A prospective cohort study was conducted at the Department of Hepato-gastroenterology, Sindh Institute of Urology and Transplant (SIUT), Karachi. The study included 209 ESRD patients on hemodialysis with non-variceal UGIB. Data collection encompassed demographic information, clinical presentations, laboratory findings, and endoscopic outcomes. Statistical analyses were performed using SPSS version 22.0, including univariate and multivariate analyses to identify independent predictors of mortality.</p><p><b><i>Results:</i></b> Among the 209 patients, 121 (57.9%) were males. The most prevalent comorbidities were ischemic heart disease (37.8%), hypertension (35.4%), and diabetes (26.8%). Endoscopic findings showed that 95.2% had gastric ulcers. Key clinical presentations included tachycardia (49.8%) and hypotension (34%). The mortality rate was 44% (92 patients). Significant predictors of mortality identified in univariate analysis included male gender (p=0.002), diabetes (p<0.001), ischemic heart disease (p<0.001), hypotension (p<0.001), tachycardia (p<0.001), and specific endoscopic findings (gastric erosions, Forest Class Ia and IIa ulcers). Multivariate analysis revealed diabetes (p=0.01), hypotension (p=0.001), tachycardia (p<0.001), Forest Class Ia ulcers (p<0.001), increased INR (p=0.009), decreased platelet count (p=0.002), and administration of dual endoscopic therapies (p<0.001) as independent predictors of mortality.</p><p><b><i>Conclusion:</i></b> ESRD patients with non-variceal UGIB have a high mortality rate. Significant predictors of mortality include diabetes, hypotension, tachycardia, specific endoscopic findings, increased INR, decreased platelet count, and the need for dual endoscopic therapy. Identifying these risk factors can guide clinical decision-making, improve management strategies, and potentially reduce mortality in this vulnerable population.</p><p><b>PP-01-162</b></p><p><b>Nurse-Driven Triage Assessment of Small Bowel Capsule Endoscopy Recordings</b></p><p><b>Tze Lee Yong</b>, Anne Davarias, Josephine Khu, Calista Koh, Liwen Xue and Suzie Ji</p><p><i>Endoscopy, Chris O'Brien Lifehouse, Sydney, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Small-bowel video capsule endoscopy (SBVCE) offers a valuable diagnostic tool for small intestinal pathologies, particularly following inconclusive gastroscopy and colonoscopy. International gastroenterological societies endorse the utilization of appropriately trained and supervised nurse endoscopists (NEs) for SBVCE image interpretation and triage. While resistance to NEs substituting for physician endoscopists exists, studies demonstrate their competence in reviewing SBVCE images. Given the rising demand for SBVCE, this paper explores the potential for a novel non-physician reader role in Australia. Specifically, discussing the feasibility of endoscopy nurses performing SBVCE pre-reading and triage, aiming to streamline workflow, optimize resource allocation and improve patient care.</p><p><b><i>Methods:</i></b> A comprehensive literature search was conducted using key terms: \"endoscopy nurse\", \"trained\", \"pre-read\", \"interpret\" and \"capsule endoscopy\". The search focused on English peer-reviewed articles published between 2017 and 2022, including systematic reviews and comparative studies that evaluated lesion detection accuracy by trained endoscopy nurses compared to gastroenterologists.</p><p><b><i>Results:</i></b> Analysis of 18 studies supports nurse-led initial review of SBVCE images where nurses achieved high accuracy, demonstrating competence. There is also improved efficiency through cost reduction and saved physician time. The medical community embraced trained nurses performing SBVCE pre-reading, with artificial intelligence (AI) showing promise for further enhancement. This evidence supports implementing nurse pre-reading for SBVCE lesion classification while maintaining diagnostic quality.</p><p><b><i>Conclusion:</i></b> This study supports the feasibility of appropriately trained and supervised endoscopy nurses performing SBVCE pre-reading and triage. Implementing this role could optimize physician time allocation, reduce healthcare costs, and improve patient access to care through shorter wait times.</p><p><b>PP-01-163</b></p><p><b>Linked color imaging versus white light imaging for the detection of gastric superficial neoplasia</b></p><p><b>Jae Gon Lee</b><sup>1</sup>, Sang Pyo Lee<sup>2</sup> and In Kyung Yoo<sup>3</sup></p><p><sup>1</sup><i>Hallym University College of Medicine, Hwaseong, South Korea;</i> <sup>2</sup><i>Hanyang University College of Medicine, Seoul, South Korea;</i> <sup>3</sup><i>CHA University, Seongnam, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The early detection of gastric superficial neoplasia (GSN) by screening endoscopy is essential, but endoscopic detection and diagnosis are sometimes difficult. Linked color imaging (LCI) can help reduce the GSN miss rate and increase the tumor detection rate compared with white light imaging (WLI). We aimed to determine whether LCI is superior to WLI for detecting and diagnosing new GSNs by screening endoscopy.</p><p><b><i>Materials and Methods:</i></b> This randomized, multi-center, prospective study was conducted on patients with suspected or diagnosed gastric adenoma or early gastric cancer. Endoscopic evaluations in the LCI and WLI groups were performed in LCI and WLI modes, respectively. After the first observation was completed in each group, additional observations were performed in a different mode. The GSN miss rate and tumor detection time and characteristics were evaluated.</p><p><b><i>Results:</i></b> Forty-eight (52 tumors) of the 95 patients included underwent LCI (the LCI group), and the remaining 47 (52 tumors) underwent WLI (the WLI group). GSN miss rates in the LCI and WLI groups were 5.77% and 13.46%, respectively. Mean tumor detection time in the LCI group was significantly shorter than in the WLI group (p=0.049). Tumors not detected by first observation were significantly more likely to have a IIb morphology (p=0.014).</p><p><b><i>Conclusion:</i></b> LCI may help reduce the GSN miss rate and enable faster detection. IIb tumors may be more difficult to detect endoscopically than other tumors.</p><p><b>PP-01-164</b></p><p><b>Loop-10 Technique for Mucosotomy Defect Closure After Esophageal Per-oral Endoscopic Myotomy (POEM)</b></p><p><b>Marc Julius Navarro</b><sup>1</sup>, Haruhiro Inoue<sup>2</sup> and Yuto Shimamura<sup>2</sup></p><p><sup>1</sup><i>Institute of Digestive and Liver Diseases, St. Luke's Medical Center, Quezon City, Philippines;</i> <sup>2</sup><i>Digestive Diseases Center, Showa University Koto Toyosu Hospital, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In more challenging esophageal per-oral endoscopic myotomy situations, mucosotomy extension, laceration and defect widening may happen. It is more technically demanding to close a large-size mucosotomy defect solely by endoscopic clips. The objective of this study is to investigate the feasibility and efficacy of Loop-10 technique for mucosotomy defect closure after esophageal per-oral endoscopic myotomy.</p><p><b><i>Materials and Methods:</i></b> Loop-10 technique were done in 30 patients for mucosotomy defect closure after esophageal per-oral endoscopic myotomy. After the execution of myotomy, the constructed first clip with loop-10 was advanced through the instrument channel of the single-channel therapeutic endoscope and was deployed in the most distal part of the mucosotomy defect. The assistant provided adequate traction and lift by pulling the support thread. Succeeding endoscopic clips were deployed to ride on the main loop, to achieve full closure of the defect. The primary outcome measure was the complete closure rate. The secondary outcome measures were sustained closure rate, defect size, total number of clips deployed, and mucosotomy defect closure time.</p><p><b><i>Results:</i></b> Complete closure and sustained closure rate of 100% were achieved. The mean mucosotomy defect length was 2.28cm (SD +/- 0.67, range 1.50-4.00cm). The mean total number of clips deployed to achieve complete closure were 3.93 clips (SD +/- 0.83, range 3-6 clips). The mean mucosotomy defect closure time was 4.80 minutes.</p><p><b><i>Conclusion:</i></b> Loop-10 technique presented to be a feasible and effective method in achieving complete closure and sustained closure of mucosotomy defect after esophageal per-oral endoscopic myotomy.</p><p><b>PP-01-165</b></p><p><b>Comparison of Magnifying Endoscopy with NBI and EUS for Predicting Tumor Invasion Depth in EGC</b></p><p><b>Jun Chul Park</b><sup>1</sup>, Soo In Choi<sup>2</sup> and Da Hyun Jung<sup>1</sup></p><p><sup>1</sup><i>Yonsei University College Of Medicine, Seoul, South Korea;</i> <sup>2</sup><i>Inje University Sanggye Paik Hospital, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In early gastric cancer (EGC), magnifying endoscopy with narrow-band imaging (ME-NBI) can predict the invasion depth by yielding clear images of the surface and microvascular pattern of tumor. We aimed to compare the diagnostic accuracy of ME-NBI in T-staging of EGC compared with conventional endoscopic ultrasonography (EUS). Material and Methods: In this prospective, randomized, non-inferiority trial, patients with EGC were randomly allocated in a 1:1 ratio to two parallel groups: ME-NBI (n=81) or EUS (n=84). The invasion depth in ME-NBI was determined based on microsurface patterns, microvascular patterns, and multicaliber vessels. Diagnostic performance for the invasion depth in each group was compared using the final histopathological diagnosis. A non-inferiority margin of -10% for the invasion depth was assumed.</p><p><b><i>Results:</i></b> The diagnostic accuracy, specificity, positive predictive value, and negative predictive value for T1sm showed no statistically significant differences between ME-NBI and EUS (66.7% versus 53.6%, p=0.0861; 64.5% versus 58.7%, p=0.5060; 38.9% versus 23.5%, p=0.1665; and 88.9% versus 74.0%, p=0.0645); however, the sensitivity was significantly higher in ME-NBI (73.7% versus 38.1%, p=0.0239). In the non-inferiority test, ME-NBI revealed non-inferiority to EUS in predicting invasion depth. Additionally, the accuracy of ME-NBI was significantly higher than that of EUS in tumors > 20 mm, depressed tumors, and T1sm1 tumors (73.2% versus 48.7%, p=0.038; 70.7% versus 46.3%, p=0.043; 71.4% versus 12.5%, p=0.041).</p><p><b><i>Conclusions:</i></b> The diagnostic capability of ME-NBI was comparable to that of EUS. ME-NBI could be a useful alternative modality for discriminating submucosal invasion of EGC, particularly in large, depressed or T1sm1 tumors.</p><p><b>PP-01-166</b></p><p><b>Erythromycin vs. Metoclopramide To Improve Endoscopy Visualization In Upper Gastrointestinal Bleeding: A Network Meta-Analysis</b></p><p><b>Adam Prabata</b><sup>1</sup>, Diski Saisa<sup>2</sup>, Isya Abiyyu Mumtaz<sup>2</sup> and Saskia Aziza Nursyirwan<sup>3</sup></p><p><sup>1</sup><i>Department Of Internal Medicine, Faculty Of Medicine, Universitas Indonesia, Jakarta, Indonesia;</i> <sup>2</sup><i>Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;</i> <sup>3</sup><i>Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The aim of this study is to compare the efficacy of erythromycin and metoclopramide for endoscopic visualization improvement.</p><p><b><i>Materials and Methods:</i></b> We searched online databases like PubMed, Scopus, and Cochrane Library, till August 20 for RCTs that compared erythromycin vs. metoclopramide with gastric lavage or placebo. We used the keywords “erythromycin”, “metoclopramide”, “endoscopy visualization”, and “upper gastrointestinal bleeding”. The risk of bias was evaluated with Cochrane Risk of Bias 2 (RoB 2). Frequentist network meta-analysis was performed to pooled the odds ratio (OR) or standardized mean difference (SMD) with 95% confidence intervals (CI). Outcomes were endoscopy visualization, repeat endoscopy, and duration.</p><p><b><i>Results:</i></b> A total 13 of RCTs that evaluated 1361 patients were included in this systematic review. Treatment consists of erythromycin, metoclopramide, control (gastric lavage or placebo), and its combination. Compared to placebo, combination of erythromycin with gastric lavage has better efficacy for satisfactory visualization (OR 9.30; 95% CI: 2.40, 36.10; I²: 38.7%) and visual endoscopy score (SMD 1.18; 95% CI: 0.44, 1.92; I²: 72.3%, but not significant for endoscopy duration (SMD 0.77; 95% CI: -0.82, 2.36; I²: 94.3%) and second look endoscopy (OR 0.41; 95% CI: 0.15, 1.17; I²: 0%).</p><p><b><i>Conclusion:</i></b> In this review, we concluded that administration of combination of erythromycin with gastric lavage could help improve endoscopy visualization in upper GI bleeding. However, high quality RCT are required to verify these findings.</p><p><b><i>Keywords:</i></b> erythromycin, metoclopramide, endoscopy visualization, upper GI bleeding, network meta-analysis</p><p><b>PP-01-167</b></p><p><b>Nasopharyngeal examination during transoral upper gastrointestinal endoscopy: Is it feasible?</b></p><p><b>Rasyiqatul Raminey</b> and Rafidah IDRIS and Sumitro Kosasih and Norwani Dewi Basir and Vui Heng Chong</p><p><i>Ripas Hospital Brunei, Bandar Seri Begawan, Brunei Darussalam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Transoral upper gastrointestinal endoscopy (UGIE) is a common investigation for upper gastrointestinal symptoms. The nasopharynx (NP) is not part of the examination but can be examined in a retroflexed position. This study assesses the feasibility of NP examinations during routine transoral UGIE.</p><p><b><i>Materials and methods:</i></b> All patients who had transoral UGIE by an experience endoscopist over a period was reviewed and analysed. A total 144 patients (mean age 53.2 ± 14.8 years, male 50.7%, overweight/obese 52.8%) were included in the study. Procedures were performed without sedation (41.7%), conscious sedation (52.8%) and general anesthesia (5.6%).</p><p><b><i>Results:</i></b> 75.7% (n=109) had adequate NP examination and visualizations of the structures were as follow: adenoid pad (100%), nasal septum (99.1%), turbinate (superior 98.2%, middle 94.5%), eustachian tubes (left 90.8%, right 84.4%), torus tubarius (right 97.2%, left 96.3%) and the fossa of Rosenmuller (right 88.1%, left 90.8%). Of the fossa of Rosenmuller, only 40.4% (right) and 47.7% (left) had adequate visualization of the bases. Reasons for failure for NP examinations included gag reflex, bleeding, including epistaxis from the adenoid pad from trauma during retroflexion, coughing and tongue movement. There was no difference between the genders (p=0.380), weight status (normal/underweight vs. overweight/obese, p=0.865), caliber of scope (9mm vs. 11mm, p=0.970) and sedation status (p=0.350 for trend) in successful NP examinations.</p><p><b><i>Conclusions:</i></b> Our study showed that NP examination is feasible during transoral UGIE with good visualization of the anatomies. There were no factors predictive of failure to examine the NP.</p><p><b>PP-01-168</b></p><p><b>“Segment Anything” with MedSAM: Delineating Barrett’s Dysplasia Using Artificial Intelligence</b></p><p><b>Jin Lin Tan</b><sup>1,2</sup>, Ka Hing Chan<sup>3</sup>, Dileepa Pitawela<sup>3</sup>, Mohamed Asif Chinnaratha<sup>1,2</sup>, Hsiang-Ting Chen<sup>3</sup> and Rajvinder Singh<sup>1,2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Lyell McEwin Hospital, Elizabeth Vale, Australia;</i> <sup>2</sup><i>Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, Australia;</i> <sup>3</sup><i>Australian Institute for Machine Learning, The University of Adelaide, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to evaluate the performance of MedSAM, a cutting-edge segmentation model trained on 1.5 million cancer images, on a dataset of Barrett’s dysplasia endoscopic images obtained using Narrow-band Imaging (NBI). Accurate segmentation is useful for highlighting subtle contour irregularities in lesions, which is applicable in computer-aided diagnostic endoscopies and speeds up the annotation of large endoscopic datasets.</p><p><b><i>Material and Methods:</i></b> A total of 248 images from 36 patients with Barrett’s dysplasia were annotated and verified against histology reports. The dataset was divided into 196 training images from 29 patients and 52 testing images from 7 patients. A model using YOLO v8 was trained to localize dysplasia areas with bounding boxes, which MedSAM used for segmentation. The performance was evaluated using the Intersection over Union (IoU) for localisation and the Dice coefficient for segmentation accuracy.</p><p><b><i>Results:</i></b> The model achieved an average IoU of 71.1% for Barrett’s dysplasia localisation, indicating a significant overlap between predicted and ground truth bounding boxes. For dysplasia segmentation, an average Dice coefficient score of 71.7% was attained, demonstrating good precision.</p><p><b><i>Conclusion:</i></b> This study demonstrates the potential of using MedSAM to accelerate and scale the annotation of large endoscopic image and video datasets, as well as its applicability to real-time segmentation in endoscopy in the future. Accurate segmentation could also help endoscopists delineate margins better prior to performing endoscopic resection.</p><p><b>PP-01-169</b></p><p><b>Optimal Management Options for Esophageal Gastrointestinal Stromal Tumors (E-GIST)</b></p><p>Shanshan Zhu, Saif Ullah and <b>Xin-Guang Cao</b></p><p><i>The First Affiliated Hospital Of Zhengzhou University, Zhengzhou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To explore the different treatment modalities for esophageal gastrointestinal stromal tumors (E-GIST) and their respective applicability and clinical outcomes.</p><p><b><i>Methods:</i></b> This is a retrospective study in which consecutive patients diagnosed with E-GIST at our hospital from January 2017 to August 2023 were included. The clinical characteristics of all the patients as well as long-term quality of life were recorded and analyzed.</p><p><b><i>Results:</i></b> A total of 23 (12 males, 11 females) E-GIST patients with a mean age of 56.7 ± 12.0 years were included in this study. Common symptoms, including upper abdominal pain, acid reflux, and heartburn, accounted for over 60% of cases. Fifteen patients underwent endoscopic resection, five patients underwent surgical resection, two patients underwent surgical resection after receiving preoperative imatinib therapy, and one patient received conservative management.</p><p><b><i>Conclusion:</i></b> Different treatment strategies may be applied to the patients with E-GIST depending on the their clinical features. Our study provides insights into precise treatment for different patients. However, due to the rarity of the disease, it is challenging to collect a large sample size from a single center, necessitating more multicenter prospective large-scale studies.</p><p><b>PP-01-170</b></p><p><b>Gastric Volvulus: A Great Masquerade</b></p><p><b>Ankit Vats</b></p><p><i>ARMY HOSPITAL R&R, New Delhi, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Dr Ankit Vats1, Col Priyank Dhiman2</p><p>1.Senior Resident</p><p>2.Associate Professor</p><p>Dept of Gastroenterology and Transplant Hepatology, Army Hospital (R&R), Delhi Cantt-110010</p><p>Gastric Volvulus: A Great Masquerade</p><p>84 year old, male patient, known case of hypertension. presented with multiple episodes of non-bilious vomiting and painful distension of abdomen to emergency department in March 2024.</p><p>On clinical examination, he had respiratory rate 28 / min , spO2 > 95 % on room air, pulse rate of 110/min regular and blood pressure of 92/60 mm of Hg. Abdominal examination revealed distension in the epigastrium and umbilical region with guarding. Bowel sounds were reduced. All the hernia sites were normal and on digital rectal examination yellow stools were present in the Rectum. A clinical diagnosis of gastric outlet obstruction was made. X Ray abdomen and chest PA view showed elevated left dome of diaphragm. UGI Endoscopy was done showing a large hiatus hernia with LA Grade D esophagitis, grossly dilated stomach with significant food residue, a swirl of mucosa was seen in the stomach extending distally, antrum could not be located. The stomach appeared to be twisted along its longitudinal axis.</p><p><b>PP-01-171</b></p><p><b>Endoscopists’ knowledge, perceptions and attitudes towards the use of Artificial Intelligence in endoscopy</b></p><p><b>Nicholas Wan</b><sup>1,2</sup>, Celine Chan<sup>1</sup>, Jin Lin Tan<sup>1,2</sup>, Asif Chinnaratha<sup>1,2</sup> and Rajvinder Singh<sup>1,2</sup></p><p><sup>1</sup><i>Gastroenterology Department, Lyell McEwin Hospital, Elizabeth Vale, Australia;</i> <sup>2</sup><i>Adelaide Medical School, University of Adelaide, Adelaide, Australia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Artificial Intelligence (AI) is evolving in endoscopy. The perceptions of endoscopists towards AI remain poorly understood. We aim to summarize the perspectives of endoscopists on AI.</p><p><b><i>Methods:</i></b> Online databases were searched to identify questionnaires conducted on endoscopists. Qualitative synthesis of included studies was conducted by categorizing into five domains: 1) Attitudes towards AI, 2) Impacts on endoscopy, 3) Impacts on endoscopists, 4) Impacts on patients, 5) Barriers towards AI implementation.</p><p><b><i>Results:</i></b> Ten studies were included in this systematic review comprising of 1587 endoscopists across Europe (32.6%), North America (42.8%), and Asia (24.6%). Domain-1: Most (69–100%) had basic knowledge of AI. Majority (79.5–87.5%) expressed interest and optimism. Domain-2: Most (62.5–97%) supported the notion that AI would positively impact endoscopic performance and quality. Domain-3: There were mixed perceptions regarding operator-dependence (6.2–62.8% agreement), and whether AI would prolong procedural time (21–81.3% agreement). Most (71–100%) disagreed that AI would replace them. Domain-4: Majority (81.3%) felt that AI would increase the quality of patient-care. Only a minority (6.2–24%) felt that AI could improve the patient-physician relationship. Domain-5: Most (75.2–91%) identified costs as potential barriers to AI implementation. Opinions on ethics and the lack of regulation varied (12.5–100% and 35–88%, respectively), with majority feeling that clear guidelines and regulations was required.</p><p><b>PP-01-172</b></p><p><b>The overlooked connection between the inadequate water intake and dyspeptic symptoms among senior citizens.</b></p><p><b>Kiran Bajaj</b>, Shahid Karim, Trainee Afsheen Faryal, Trainee Afsheen Faryal and Afsheen Faryal</p><p><i>Liaquat National Hospital, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Dyspepsia characterized by discomfort in upper abdomen. One of the potential risk factors causing dyspepsia is dehydration.</p><p><b><i>Study Aim and objective:</i></b> The aim of study is to investigate the water intake habit among the different age groups population and its association with dyspeptic symptoms by assessing the relationship of adequate water intake with dyspepsia according to SF-LEEDS questionnaire.</p><p><b><i>Patients and Methods:</i></b> Cross Sectional, prospective study was conducted, in 2021-22, at the Department of Gastroenterology, Liaquat National Hospital, Karachi. Total 450 patients were enrolled for study after informed consent. Dyspepsia diagnosed by SF-LEEDS questionnaire with 32 max score. It was categorized into mild, moderate and severe with score of 10, 11-20, 21 respectively.</p><p><b><i>Results:</i></b> Total 450 patients were enrolled in study with median age of 43 (IQR= 30-55) years. And (58.7%) were males. Patients presented with mild (n=19, 4.2%), moderate (n=95, 21.1%) and severe dyspepsia (n=336, 74.7%) with median Leed’s score of 20 (IQR= 17-22). Sign of dehydration including dry tongue (n=175, 38.9%), tachycardia (n=13, 2.9%) and reduce skin turger (n=3, 0.7%) were present among studied patients. Median daily water intake, recommended water intake, percent of having recommended water and SFLD score was 6 (5-7) glasses, 8.8 (7.5-10.16) glasses, 68.87% (54.76-84.18) and 20 (17-22) respectively. Our study showed that overall daily water intake habit was around 1-2 liters i.e., 50%-80% of the recommended daily water intake.</p><p><b>PP-01-173</b></p><p><b>Association between esophageal baseline impedance levels and reflux parameters suggesting defensive mechanisms</b></p><p><b>Sang Hyuk Jung</b> and Seon-Young Park</p><p><i>Chonnam National University, Gwangju, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Ambulatory multichannel intraluminal impedance-pH monitoring (MII-pH) and high-resolution esophageal manometry (HRM) are commonly performed to objectively assess pathologic reflux and understand the pathomechanism in individuals with reflux symptoms. This study aimed to investigate the relationship between novel MII-pH parameters and other metrics in patients experiencing refractory reflux symptoms.</p><p><b><i>Methods:</i></b> This retrospective study included patients with persistent reflux symptoms who underwent both HRM and MII-pH assessments. We evaluated total acid exposure time (AET), total bolus exposure time (BET), total number of reflux episodes, post-reflux swallow-induced peristaltic wave index (PSPW-I), and mean nocturnal baseline impedance (MNBI) from MII-pH data. The mean distal contractile integral (DCI) during swallows was derived from HRM.</p><p><b><i>Results:</i></b> In this cohort of 55 patients (30 females, median age 58 years), 9 (16.4%) exhibited AET >6.0%, 16 (29.1%) experienced >80 reflux episodes, and 7 (12.7%) had ineffective esophageal motility. Median (10%–90%) MNBI values for Z5 and Z6 were 2140 (258–4046) ohms and 1680 (158–3994) ohms, respectively. Median PSPW-I (10%–90%) was 0.31 (0.07–0.59). MNBI at Z5 and Z6 showed positive correlations with DCI and negative correlations with ineffective swallows, AET, BET, acid clearance time, bolus clearance time, and total reflux episodes (P<0.05). PSPW-I exhibited positive correlations with DCI and negative correlations with ineffective swallows, AET, BET, total reflux episodes, and bolus clearance time (P<0.05). Additionally, MNBI values at Z4, Z5, and Z6 correlated with PSPW-I (P<0.05).</p><p><b><i>Conclusions:</i></b> MNBI and PSPW-I are associated with both the quantitative reflux index and reflux parameters suggesting defensive mechanisms.</p><p><b>PP-01-174</b></p><p><b>Long-term outcomes after therapeutic induction in patients with functional dyspepsia</b></p><p><b>氏 Takayuki Ksitano</b>, Toshiko Tomita, Masatoshi Mieno, Hideki Yoneda, Norio Nishii, Kumiko Nakamura, Maiko Ikenouchi, Yoshitaka Kitayama, Hiroo Sei, Hirotsugu Eda, Koji Kamikozuru, Yoko Yokoyama, Takuya Okugawa, Hirokazu Fukui and Shinichiro Shinzaki</p><p><i>Hyogo Medical University, Nishinomiya, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> Quality of life (QOL) of patients with functional dyspepsia is known to be impaired. However, the long-term outcome in gastrointestinal symptoms and QOL after therapeutic induction in patients with FD is unclear. We aimed to clarify long-term outcome including gastrointestinal symptoms and QOL in patients with FD after therapeutic induction.</p><p><b><i>Methods:</i></b> We enrolled 111 FD patients whose clinical data were traceable for 2 years after therapeutic induction. Gastrointestinal symptoms and QOL were assessed using GSRS, HADS and SF-8 questionaries before and after treatment.</p><p><b><i>Results:</i></b> The improvement rate of symptoms in patients with FD was 55% and 78% at 1 or 2 years after treatment, respectively. After 1-year treatment, the initial diarrhea score in GSRS and depression score in HADS before treatment were significantly higher in FD patients without improvement than in those with improvement (p < 0.05), whereas SF-8 did not differ between two groups. After 2 years of treatment, the initial GSRS including diarrhea, abdominal pain and dyspepsia and initial total HADS score before treatment were significantly higher in FD patients without improvement than in those with improvement (p < 0.05). In addition, physical component summary on SF8 was significantly lower in FD patients without improvement.</p><p><b><i>Conclusions:</i></b> Only half of patients with FD achieve complete symptom improvement at after 1-year treatment. The patients who had high HADS scores and GSRS (diarrhea and abdominal pain) showed no significant improvement in their gastrointestinal symptoms.</p><p><b>PP-01-175</b></p><p><b>Bile Reflux Gastropathy in Adult Filipinos with Dyspepsia</b></p><p><b>Ronell Lee</b> and Tan</p><p><i>Chinese General Hospital And Medical Center, Manila, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Bile Reflux Gastropathy in Adult Filipinos with Dyspepsia</p><p>LEE Ronell; TAN Jose</p><p>Chinese General Hospital and Medical Center</p><p><b><i>Objectives:</i></b> This study aims to determine the prevalence of Bile Reflux Gastropathy (BRG) among adult Filipinos with dyspepsia. Secondary objectives include comparing endoscopic findings between patients with and without BRG, associating BRG presence with patient symptoms, and identifying risk factors for BRG.</p><p><b><i>Materials and Methods:</i></b> This analytical cross-sectional prevalence study will include adult Filipinos with dyspepsia scheduled for Esophagogastroduodenoscopy (EGD) at the Endoscopy Unit of Chinese General Hospital. Patients aged 19 and older meeting Rome IV criteria for functional dyspepsia will be included. Exclusion criteria will comprise patients with previous stomach, gallbladder, or small bowel surgery and those unable to communicate effectively. Consecutive sampling will be employed until the sample size of 176 is reached. Data will be collected from pre-procedural endoscopy forms, endoscopy reports, images, and videos. Statistical analyses will include Pearson’s chi-squared test and independent t-test with a significance level set at p ≤ 0.05.</p><p><b><i>Results:</i></b> It is anticipated that BRG will be identified in a significant proportion of the 176 patients. Patients with BRG are expected to exhibit more severe endoscopic findings and symptoms such as postprandial fullness and epigastric pain. Anticipated risk factors for BRG include NSAID use and Helicobacter pylori infection.</p><p><b><i>Conclusion:</i></b> The anticipated prevalence of BRG in adult Filipinos with dyspepsia is notable, with expected significant correlations between BRG, endoscopic findings, and patient symptoms. Identifying BRG is crucial for tailored management strategies to improve patient outcomes.</p><p><b>PP-01-176</b></p><p><b>Associations of Atrophic Gastritis and Intestinal Metaplasia with Refractory/Recurrent Epigastric Pain-A Retrospective Cohort Study</b></p><p><b>Chan Yi Lin</b><sup>1</sup>, Jyh-Ming Liou<sup>2</sup> and Tzu-chan Hong<sup>2</sup></p><p><sup>1</sup><i>National Taiwan University Hospital, Taipei, Taiwan;</i> <sup>2</sup><i>Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital Cancer Center, Taipei, Taiwan, Taipei, Taiwan;</i> <sup>3</sup><i>Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital Cancer Center, Taipei, Taiwan, Taipei, Taiwan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to explore the correlation between the severity of gastric atrophy (GA) and gastric intestinal metaplasia (GIM) and the occurrence of symptoms, particularly refractory and recurrent epigastric pain.</p><p><b><i>Materials and Methods:</i></b> Data from 431 patients who underwent endoscopy for upper GI symptoms at the National Taiwan University Cancer Center from January 2022 to December 2023 were analyzed. Clinical characteristics, symptoms, endoscopic findings, and pathology results were collected, including refractory or recurrent pain post-acid suppression therapy. GA and GIM were assessed using the OLGA and OLGIM grading systems. Statistical analyses included Pearson’s Chi-Square and Kruskal-Wallis ANOVA, with a significance threshold of p < 0.05.</p><p><b><i>Results:</i></b> The mean age was 62.6 years, with 48.3% male. Epigastric pain occurred in 59.2% of patients, with 15.1% reporting refractory pain and 6.2% having recurrent pain. Increased GA and GIM severity correlated with older age. GIM (OLGIM stage 1-4) was associated with more symptoms (p = 0.0143) but not recurrent pain. GA (OLGA stage 1-4) was linked to recurrent pain (p = 0.0208) but not refractory pain. Multivariable analysis found GIM associated with age (OR 1.10, p = 0.0011) and H. pylori infection (OR 7.15, p = 0.0013), while GIM severity was linked to refractory pain (OR 16.11, p = 0.0189).</p><p><b><i>Conclusion:</i></b> This study highlights significant correlations between GA, GIM, and symptoms. Refractory pain was associated with GIM severity, while recurrent pain was linked to GA severity. These findings underscore the importance of considering gastric pathology in symptom evaluation and early detection strategies.</p><p><b>PP-01-177</b></p><p><b>COMPARATIVE STUDY OF GASTROINTESTINAL GAS VOLUME IN PATIENTS WITH FD BASED ON CT AND GVS</b></p><p><b>Cong Liu</b>, Doctor Bin Whang, Doctor Lili Zhang, Doctor Wei Zhao, Doctor Hong Jin, Shiwei Ru, Shujin Li and Xuechai Liu</p><p><i>Tianjin Medical University General Hospital, Tianjin, CN, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To compare the efficacy of the gas volume score (GVS) and computed tomography (CT) imaging combined with a calculation formulain detecting gastrointestinal gas volume among patients with functional dyspepsia (FD).</p><p><b><i>Materials and Methods:</i></b> December 1, 2021 to June 30, 2022, 27 FD patients, as the FD group, and 30 healthy individuals, as the control group were admitted from Tianjin Medical University General Hospital from. Both groups underwent X-rays and CT scans, with subsequent evaluation of gastrointestinal multi-site gas volume using the GVS and CT methods. Gastrointestinal multi-site gas volume of the different subtypes of FD were also compared.</p><p><b><i>Results:</i></b> Based on the GVS,small intestine and colorectal gas volumes were higher in the FD group than the healthy control group (P = 0. 001 and <0. 001). But,there were no significant differences in the gas volume among different sub types. Based on the CT method, the gas volume in the stomach and colon of the FD group was higher than that of the healthy control group, and the differences were statistically significant (P=0.006, 0.001); In addition, PDS patients exhibited higher gastric gas volume than EPS patients (P=0.038), which was not seen by GVS.</p><p><b><i>Conclusion:</i></b> The amount of gastrointestinal gas in FD patients is higher than that in control group. The gas accumulation in the gastric cavity of PDS patients is significantly greater than that in EPS patients. The CT method can help to calculate the gas volume in the gastrointestinal tract of FD patients more accurately.</p><p><b>PP-01-178</b></p><p><b>Nonsteroidal Anti-inflammatory Drug Gastropathy: Correlation between The Degree of Gastric Mucosal Injury and Endoscopic Appearances</b></p><p><b>Meti Metiani</b><sup>1</sup>, Dolvy Girawan<sup>2</sup>, Muhammad Begawan Bestari<sup>3</sup>, Nenny Agustanti<sup>4</sup> and Eka Nugraha<sup>5</sup></p><p><sup>1</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>3</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>4</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>5</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aim to assess the correlation between the degree of gastric mucosal injury, as determined by endoscopic examination (Lanza score) and histopathological examination (Sydney classification), while also considering the impact of patient characteristics.</p><p><b><i>Materials and Methods:</i></b> From January 2022 to June 2024, we conducted a retrospective cross-sectional study, sampling a total of 386 adult patients (over 18 years of age) who had symptoms of dyspepsia or upper gastrointestinal bleeding, were taking NSAIDs and underwent endoscopic procedures and biopsy. We classified endoscopy results using the Lanza score. Histopathology results were categorized by the Sydney classification. We conducted statistical analysis using the chi-square and Spearman correlation tests.</p><p><b><i>Results:</i></b> There was a positive correlation between the Lanza score and the degree of inflammation (r=0.198; p<0.001), neutrophil activation (r=0.208; p<0.001), glandular atrophy (r=0.173; p=0.001), and intestinal metaplasia (r=0.096; p=0.058). However, it did not correlate with the density of H. pylori infection (r=0.014; p=0.783). The higher the patient's age category, the more severe the injury with the Lanza score (r=0.156; p<0.05) and the greater the possibility of experiencing an ulcer (r=0.068, p=0.180), but increasing age did not have a significant effect on the Sydney classification. Gender did not affect gastric mucosal injury, whether assessed by the Lanza score or Sydney classification.</p><p><b><i>Conclusion:</i></b> The more severe the injury to the gastric mucosa, the greater the degree of inflammation. Increasing age can also affect the severity of mucosal abnormalities, but neither gender nor the H. pylori infection influence their severity.</p><p><b>PP-01-179</b></p><p><b>Vitamin D Supplementation Improving Stress Level, Autonomic Nervous System,and Inflammatory Markers in Functional Dyspepsia</b></p><p><b>Ari Prasetyo Nugroho</b>, Triyanta Yuli Pramana, Aritantri Darmayani and Didik Prasetyo and K. Psi Ratih Arianita</p><p><i>Rsud Dr.moewardi Surakarta, Surakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Impairment in gut-brain communication is one of the main causes functional dyspepsia (FD). FD might be associated with autonomic dysfunction, which can be identified via heart rate variability (HRV). It has been suggested that FD may be associated with decreased levels of vitamin D. Recent studies demonstrated that vitamin D supplementation improved depression and anxiety, which are common psychological comorbidities in patients with FD. Therefore, we investigated whether vitamin D supplementation effectively improved the FD symptoms, ANS imbalanced, and inflammatory markers in patients with FD.</p><p><b><i>Method:</i></b> This is an experimental study with a one-group pretest and post-test design. FD scoring by the NDI scale and stressor scale scoring with STAI-S and STAI-T. Inflammatory markers (NLR, RDW, and PLR) and HRV were performed in all subjects before and after vitamin D supplementation for 1 month.</p><p><b><i>Results:</i></b> The study, which included 25 patients, 20 women and 5 men showed there was a significant effect of vitamin D on reducing the NDI score in FD patients (p < 0.001). The study also showed a decrease in the STAI stressor level and an improvement in the SDNN with p < 0.001, and there was an increase in the LF/HF value, but was not significant. Vitamin D reduced inflammatory markers, especially NLR, PLR, and RDW (p = 0.183, p = 0.626, p = 0.740), respectively. However in this study, the decrease in these values was not significant.</p><p><b>PP-01-180</b></p><p><b>Vitamin d play a role in stabilizing the level of stress and sympathovagal reflex balance</b></p><p><b>Triyanta Yuli Pramana</b><sup>1</sup>, K. Psi Ratih Arianita<sup>2</sup>, Aritantri Darmayani<sup>1</sup>, Didik Prasetyo<sup>2</sup>, Apriliana Adhyaksari<sup>3</sup> and Ari Prasetyo<sup>3</sup></p><p><sup>1</sup><i>Division Of Gastroenterohepatology Internal Medicine Department, Faculty Of Medicine Sebelas Maret University Dr. Moewar, Surakarta, Indonesia;</i> <sup>2</sup><i>Division of Psychosomatic and Palliative Medic Internal Medicine Department, Faculty of Medicine Sebelas Maret University Dr. Moewardi Hospital, Surakarta, Surakarta, Indonesia;</i> <sup>3</sup><i>Subspecialist Program Division of Gastroenterohepatology Internal Medicine Department, Faculty of Medicine Sebelas Maret University Dr. Moewardi Hospital, Surakarta, Surakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Functional dyspepsia (FD) is a common functional disease that includes a disorder of gut-brain interaction. Psychosocial factors are considered to play an important role and can also be associated with autonomic nervous system (ANS) dysfunction. Vitamin D is found to be important not only for physical health problems but also to address various mental health issues, especially reduction in the occurrence of negative emotions. Vitamin D also has immunomodulatory properties that might be useful in FD. In this research, we want to prove the efficacy of vitamin D in stress level improvement and sympathovagal reflex balance.</p><p><b><i>Methods:</i></b> A total of 25 FD subjects underwent an HRV examination to record the changes of sympathovagal activity. The ANS balance is described through RMSSD, Total Power (TP) score, and PSI. Stress Level was measured by PSS-10. All examinations are carried out before and after being given vitamin D on all subjects. All data was analyzed using Wilcoxon Rank Test and Paired t-test.</p><p><b><i>Results:</i></b> Vitamin D had a significant effect on improving PSS-10 score (P<0.001) which indicated a decrease stress levels in FD patients. Vitamin D also improved HRV levels which was described by a decrease in PSI (P<0.001); increase in TP (P<0.001) and increase in RMSSD (p<0.001).The ANS stability also decreased, indicating there was a better balance was achieved (p=0.002) and the activity of ANS also increased (p=0.028).</p><p><b>PP-01-181</b></p><p><b>Correlation between Lifestyle and Food Preferences with Gastritis Diagnosis Based on Endoscopic Results in Makassar</b></p><p><b>Muhammad Azhary Eka Putra</b><sup>1</sup>, Fardah Akil<sup>2,3</sup>, Susanto Hendra Kusuma<sup>2,3</sup>, Muhammad Luthfi Parewangi<sup>2,3</sup>, Nu'man AS Daud<sup>2,3</sup>, Rini Rachmawarni Bachtiar<sup>2,3</sup> and Amelia Rifai<sup>2,3</sup></p><p><sup>1</sup><i>Internal Medicine of Hasanuddin Univerisity, Makassar, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Hasanuddin University, Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia;</i> <sup>3</sup><i>Centre of Gastroenterology-Hepatology HAM Akil, Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gastritis is a common gastrointestinal disorder characterized by inflammation of the stomach lining, leading to symptoms such as abdominal pain, discomfort, nausea, and loss of appetite. The role of lifestyle and dietary habits in the development and management of gastritis is crucial but not fully understood. This study aims to examine the correlation between lifestyle and dietary habits with diagnosis of gastritis based on endoscopic results, identifying significant dietary factors that influence the likelihood of developing various types of gastritis.</p><p><b><i>Materials and Methods:</i></b> A cross-sectional analysis was conducted using medical record data and endoscopic reports. Logistic regression was used to analyze the relationship between six independent variables—fiber intake, fatty food intake, alcohol consumption, NSAID overused, and smoking—and diagnose of gastritis based on European Society of Gastrointestinal Endoscopy (ESGE) Consensus. Data preprocessing included normalizing variables on a 0-100 scale to 0-1 and encoding binary variables.</p><p><b><i>Results:</i></b> This study involved 92 patients with various clinical manifestations of gastritis who had undergone endoscopy. There are 49 male patient and 43 female patient. Significant associations were found between dietary habits and predictor diagnostic of gastritis. Fiber intake had varied impacts on different gastritis types. Alcohol consumption and NSAID use were positively correlated with increased gastritis risk. The logistic regression models showed moderate to good performance, with varied influences of dietary fats and smoking on gastritis types.</p><p><b>PP-01-182</b></p><p><b>Role of Psychological Distress in Functional Dyspepsia: Relationship between NDI, HADS,PSS-10 and ANS</b></p><p><b>Stefanus Ranty</b>, Triyanta Yuli Pramana, K. Psi Ratih Arianita, Aritantri Darmayani and Didik Prasetyo</p><p><i>RS Dr. Moewardi, Surakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Functional dyspepsia (FD) defined as chronic or recurrent upper abdominal pain or discomfort without any structural abnormalities in the gastrointestinal tract. Psychological distress is understood to be associated with FD, and both distress and anxiety can precede symptoms and the symptoms can induce stress and anxiety. This study aims to evaluate the relationship between the degree of severity of FD, measured by NDI, and degree of psychological factors, calculated by HADS and PSS-10, and also between NDI and imbalance of ANS's activity (SNS and PNS, PSI, ANS balance and ANS activity, measured with Heart Rate Variability (HRV))</p><p><b><i>Materials and Methods:</i></b> This is a cross-sectional study, involving 25 FD patients, filling the NDI criteria, HADS, and PSS-10. Heart rate variability was measured, and all variables needed were collected, all in one time meeting. All data was analyzed, using Correlation Eta test, Spearman Rank and/or Pearson Product Moment.</p><p><b><i>Results:</i></b> There is positive correlation between NDI and PSS-10 (r=0.515, p=0.008) and NDI with HADS-A (r=0.542, p=0.005), while no significant correlation with HADS-D (r=0.327, p=0.111). the NDI is also not correlated with SNS&PNS, PSI, ANS Balance, and ANS activity. (r=0.099, p-0.638; r=0.041, p=0.846; r=0.164, p=0.434; r=-0.096, p=0.647 respectively</p><p><b>PP-01-183</b></p><p><b>Significance related diseases of gastric intestinal metaplasia in children and adolescents: preliminary survey</b></p><p><b>Eell Ryoo</b></p><p><i>Gachon University, Gil Medical Center, Incheon, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastric intestinal metaplasia (GIP)is recommended for regular examination as precancerous lesions associated with increased risk of gastric cancer in adults, but there are few studies related to this in children and adolescents. Through a preliminary study, the prognosis was to be followed up for an observable period.</p><p><b><i>Method:</i></b> From 2001 to July 2024, patients who visited the hospital due to gastrointestinal symptoms and performed gastric biopsy along with upper gastrointestinal endoscopy were analyzed retrospectively.</p><p><b><i>Results:</i></b> Of 7,979 patients (65,733 cases), 3,533 patients (7,707 cases; 39.3%) underwent upper gastrointestinal endoscopy and biopsy.</p><p>Of histologic findings, 31 cases (0.88%) showed GIP, 14 cases were male, the mean age was 13.01±3.70 years old, and the BMI was 19.2±3.3 kg/m2. The chief complaints were abdominal pain in 25 cases, nausea or vomiting in 4, and abdominal discomfort in 2. As a result of endoscopy, 10 erosive gastritis, 6 hemorrhagic gastritis, 4 gastric and duodenal ulcer, 4 reflux esophagitis, 3 normal, 2 bile reflux gastritis, 1 polyps, and 1 nodular gastritis. Of these, H. pylori positive was 23% (7 cases). The final diagnosis was 9 acute gastritis, 7 H. pylori gastritis, 4 reflux esophagitis, 3 gastric and duodenal ulcer, 3 functional gastrointestinal disease, 2 Crohn's disease, 2 bile reflux gastritis, and 1 tubular adenoma.</p><p>There were 15 patients who underwent follow-up endoscopy, and in two of these cases, intestinal metaplasia persisted on follow-up examination.</p><p><b><i>Conclusion:</i></b> GIP is very rare in children and adolescents and seems to be highly related to H. pylori infection.</p><p><b>PP-01-184</b></p><p><b>Effective Treatment of Chronic Atrophic Gastritis with Umbilical Cord Mesenchymal Stem Cells Submucosal Injection</b></p><p><b>Qianqian Xu</b>, Mingyue Liu and Hongwei Xu</p><p><i>Department of Gastroenterology, Shandong Provincial Hospital, Shandong, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate the therapeutic effect of endoscopic submucosal injection of human umbilical cord mesenchymal stem cells (hUC-MSCs) on chronic atrophic gastritis (CAG) in rabbits and investigate the impact of hUC-MSCs on atrophy using a co-culture system.</p><p><b><i>Materials and Methods:</i></b> HUC-MSCs were isolated from human umbilical cord tissue, characterized by morphology, surface markers, and differentiation abilities. CAG in rabbits was induced using MNNG, ammonia, sodium salicylate, and ranitidine. After 12 weeks, endoscopic examinations, biopsies, and serum markers (G-17, PG I, PG II, PG I/II) were assessed. HUC-MSCs were submucosally injected via endoscopy; controls received saline. Treatments were administered twice, 4 weeks apart, with evaluations on days 30 and 60 post-initial injection. CAG cell model (MC cells), induced using MNNG-stimulated GES-1 cells, were co-cultured with hUC-MSCs in a Transwell co-culture system. Apoptosis was assessed by flow cytometry, autophagosomes by immunofluorescence, and protein markers of EMT, apoptosis, and autophagy by Western blot.</p><p><b><i>Results:</i></b> HUC-MSCs showed spindle-shaped morphology, expressed MSC markers, and differentiated into adipogenic, osteogenic, and chondrogenic lineages in vitro. Model rabbits exhibited significant gastric mucosal changes and decreased serum markers. HUC-MSCs injection increased serum marker levels, improved gastric mucosa appearance, alleviated glandular atrophy, restored mucosal structure, and inhibited inflammation. Saline controls showed no significant improvement. Furthermore, HUC-MSCs inhibited apoptosis and EMT in MC cells and promoted autophagy.</p><p><b>PP-01-185</b></p><p><b>Two Cases of Endoscopic Antireflux Mucoplasty (ARMP) Using the Reopenable-Clip Over the Line Method (ROLM)</b></p><p><b>Satoshi Asai</b>, Kento Hisamatsu, Yuma Fujita and Kotaro Takeshita</p><p><i>Dep. Gastroenterology, Tane General Hospital, Osaka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Endoscopic antireflux mucosectomy (ARMS) for patients with proton pump inhibitor (PPI)-refractory gastroesophageal reflux disease (GERD) has been covered by the national insurance system in Japan since 2022. ARMP is a procedure in which the post-ARMS ulcer is closed using an endoscopic closure technique. ARMP is expected to reduce post-procedure hemorrhage and provide more rapid symptom improvement compared to ARMS. However, endoscopic closure of post-ARMS ulcers is technically demanding due to the challenging maneuverability of the endoscope.</p><p><b><i>Case Description:</i></b> Case 1: A fifty-four-year-old female with a history of sleeve gastrectomy was suffering from PPI-refractory regurgitation. Esophagogastroduodenoscopy (EGD) revealed reflux esophagitis (LAclassification M), 2 cm cardiac opening (CO), and 3 cm sliding hernia (SH). pH monitoring on PPI indicated frequent reflux and associated symptoms. Therefore, ARMP using the ROLM was performed (procedure time: 74 minutes). The patient's symptoms improved immediately after the procedure without any adverse events.</p><p>Case 2: A seventy-five-year-old male was suffering from PPI-refractory regurgitation and heartburn. EGD revealed reflux esophagitis (LA classification C), 2.5 cm CO, and 2 cm SH. pH monitoring on PPI showed values at the upper limit of the reference range. Given his significant reflux symptoms and desire for the procedure, ARMP using the ROLM was performed (procedure time: 60 minutes). The patient's symptoms partially improved immediately after the procedure without any adverse events.</p><p><b><i>Discussion:</i></b> ARMP using the ROLM was relatively easy to perform and is considered feasible.</p><p><b>PP-01-186</b></p><p><b>Our Experience of Anti-Reflux Mucosectomy; ARMS for Refractory Gastroesophageal Reflux Disease</b></p><p><b>Yoshitaka Hata</b><sup>1,2</sup>, Hiroki Fukuya<sup>1</sup>, Hirotaka Tsuru<sup>1</sup>, Masafumi Wada<sup>1</sup>, Bai Xiaopeng<sup>1</sup>, Yosuke Minoda<sup>1</sup>, Yoshimasa Tanaka<sup>1</sup>, Haruei Ogino<sup>1</sup> and Eikichi Ihara<sup>1</sup></p><p><sup>1</sup><i>Kyushu University, Fukuoka, Japan;</i> <sup>2</sup><i>Saiseikai Futsukaichi hospital, Cikushino city, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Backgrounds:</i></b> Gastroesophageal reflux disease (GERD) is mainly caused by gastric acid reflux, and the primary treatment is acid secretion inhibitor such as proton pump inhibitor (PPI). Recently, a strong acid secretion inhibitor, vonoprazan, has been developed, but a subset of patients exhibits refractory GERD, which presents a clinical challenge. The pathogenesis of refractory GERD involves insufficient acid secretion suppression, non-acid reflux, esophageal hypersensitivity and altered esophageal motility. Therefore, multichannel intraluminal impedance/pH monitoring (MII/pH) and high-resolution manometry (HRM) are indispensable to evaluate refractory GERD. In addition, endoscopic anti-reflux therapy such as anti-reflux mucosectomy (ARMS) has been developed and is anticipated to offer another promising treatment for refractory GERD in Japan.</p><p><b><i>Methods:</i></b> This study aimed to evaluate the safety and effectiveness of ARMS in introduction phase. Seven patients who underwent ARMS for refractory GERD in Kyushu university hospital between April 2022 and March 2024 were included. Patients' pre- and post-ARMS symptom scores and examination results were evaluated.</p><p><b><i>Results:</i></b> Median age was 68 years with male to female ratio 4:3. Six patients were administered vonoprazan and one patient were administered PPI. All patients exhibited cardiac opening with MII/pH identifying 4 with non-erosive reflux disease and 3 with reflux hypersensitivity. The ARMS procedures were successfully completed in all patients, with the F-scale improving from 15 to 7. MII/pH also demonstrated a reduction in reflux frequency and lower esophageal acid exposure time.</p><p><b><i>Conclusion:</i></b> ARMS can be an effective treatment for refractory GERD, after excluding functional heartburn and esophageal motility disorders not associated with reflux.</p><p><b>PP-01-187</b></p><p><b>Erosive Esophagitis Status in A High-Risk Area of Gastric Cancer in Indonesia</b></p><p><b>Ryan Herardi</b><sup>1,2</sup>, Ari Fahrial Syam<sup>3</sup>, Muhammad Miftahusurrur<sup>4</sup>, Hasan Maulahela<sup>3</sup>, Agustinus Taolin<sup>5</sup>, Rasco Sandy Sihombing<sup>5</sup>, Ahmad Yusran<sup>1</sup>, Gaberia Gaberia<sup>5</sup>, Nathaniel Jason Zacharia<sup>5</sup>, Iqbal Taufiqqurachman<sup>6</sup> and Astried Monica<sup>7</sup></p><p><sup>1</sup><i>Gastroenterohepatology Fellowship. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;</i> <sup>2</sup><i>Faculty of Medicine, UPN Veteran Jakarta, Jakarta, Indonesia;</i> <sup>3</sup><i>Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;</i> <sup>4</sup><i>Division of Gastroenterohepatology. Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>5</sup><i>Mgr. Gabriel Manek Hospital, Atambua, Belu, Indonesia;</i> <sup>6</sup><i>Research Staff. Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;</i> <sup>7</sup><i>Research Staff. Department of Internal Medicine, Faculty of Medicine, UPN Veteran Jakarta, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Helicobacter pylori infection (HPI) is a high-risk factor for gastric cancer. This study aims to assess the prevalence of erosive esophagitis in populations with a high rate of HPI and evaluate their associations</p><p><b><i>Materials and Methods:</i></b> A cross-sectional study was conducted at Mgr Gabriel Manek Hospital, Atambua, Belu, East Nusa Tenggara, Indonesia from May 27th to June 8th, 2024. Patients with persistent upper gastrointestinal discomfort (>3 months) were included. All participants ceased consuming proton pump inhibitors, H2 antagonists, Non-Steroid Anti Inflammation Drugs, and steroids for at least four weeks before the examination. Esophagogastroduodenoscopy and Urea Breath Test were performed to diagnose erosive esophagitis and HPI. The study excluded pregnant patients and those with autoimmune disease, immunosuppression, kidney disease, biliary disease, hepatic cirrhosis, or upper gastrointestinal obstruction. A chi-square analysis examined the association between erosive esophagitis and HPI.</p><p><b><i>Results:</i></b> A total of 117 patients were assessed, with 65 (55.6%) female. Of these, 60 patients (51.3%) were within 20 to 40 years. Among these patients, 37.6% presented with erosive esophagitis, and 41% of patients were found to have HPI. The prevalence of erosive esophagitis was categorized as follows: grade A in 35 patients (29.9%), grade B in 8 patients (6.8%), and grade C in 1 patient (0.9%). A chi-square analysis revealed a p-value of 0.683, indicating no significant association between erosive esophagitis and HPI.</p><p><b><i>Conclusion:</i></b> Despite a high Helicobacter pylori infection rate, there is no protective correlation with the prevalence of erosive esophagitis in this rural area.</p><p><b>PP-01-188</b></p><p><b>Esophageal Adenocarcinoma in Long Segment Barrett’s Esophagus extending ≥10 cm</b></p><p><b>博士 Tomohiro Inoue</b> and Mamoru Ito and Masao Yoshioka and Satsuki Yoshinari and Ryouichi Harada and Daisuke Kawai and Keita Harada and Shuhei Ishiyama and Akiko Fujiwara and Junichirou Nasu and Junji Shiode</p><p><i>Okayama Saiseikai General Hospital, Dept. of Gastroenterology, Okayama, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> ESGE guidelines recommend 3-year surveillance for Barrett's esophagus (BE) of 3-10 cm and referral to specialty centers for BE ≥10 cm. Discontinuation of surveillance is suggested for patients ≥75 years. In Asia, adenocarcinoma is relatively rare compared to squamous cell carcinoma. In Japan, targeted biopsy is more common due to the widespread use of magnifying endoscopy, and surveillance is often done at gastroenterology clinics rather than specialized centers, with an average lifespan of 84 years. Uniform application of European standards may be challenging in this context.</p><p><b><i>Materials and Methods:</i></b> This study evaluated ESGE guidelines by analyzing esophageal adenocarcinoma cases from BE ≥10 cm at Okayama Saiseikai General Hospital (January 2011-March 2024). Data included patient demographics, Barrett's adenocarcinoma risk factors (GERD, dysplasia, obesity, smoking, alcohol, family history), diagnostic trigger, BE-to-adenocarcinoma interval, and maximum BE length.</p><p><b><i>Results:</i></b> There were four patients. All patients were male, aged 67-92 years (median 74). The mean length of BE was 10.8 cm. Diagnoses occurred 12 years after pharyngoesophageal discomfort, 21 years after appetite loss, 8 years after surveillance, and incidentally at the first screening respectively. Risk factors: GERD (4/4), dysplasia (1/4), obesity (1/4), smoking (1/4), alcohol (1/4), and family history (0/4).</p><p><b><i>Conclusion:</i></b> For high-risk patients with BE ≥10 cm, BE-to-adenocarcinoma intervals varied significantly. Two patients over 75 years developed adenocarcinoma, challenging the guideline for discontinuation of surveillance. Targeted biopsies may ease the burden on elderly patients. Long-term surveillance is crucial for LSBE, and further research is needed to refine surveillance methods, intervals, and age limits.</p><p><b>PP-01-190</b></p><p><b>Relationship brtween oral acid or burning sensation and gastroerophageal reflux</b></p><p><b>Cong Liu</b><sup>1</sup>, Doctor Lili Zhang<sup>1</sup>, Doctor Wei Zhao<sup>1</sup>, Doctor Bin Whang<sup>1</sup>, Doctor Hong Jin<sup>1</sup>, Xuechai Liu<sup>1</sup>, Shujin Li<sup>1</sup>, Shiwei Ru<sup>1</sup> and Jinfeng Zhang<sup>2</sup></p><p><sup>1</sup><i>Tianjin Medical University General Hospital, Tianjin CN, China;</i> <sup>2</sup><i>Tianjin Fourth Cent Hosp, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the relationship between oral acid/burning sensation and gastroesophageal reflux disease(GERD) using salivary pepsin detection.</p><p><b><i>Materials and Methods:</i></b> From May to June, 2023, 116 patients were enrolled consecutively at the endoscopy center of Tianjin Medical University General Hospital. 20 patients with oral acid/burning sensation (symptom group) and 96 patients without oral acid/burning sensation (control group) underwent a symptom questionnaire survey, salivary pepsin detection, and electronic gastroscopy. Salivary pepsin positivity, the prevalence of GERD, and scores of anxiety and depression were compared between the two groups.</p><p><b><i>Results:</i></b> 17.2% patients suffered from oral acid/burning sensation. Salivary pepsin test positivity and GERD Q scores were significantly higher in the symptom group than in the control group(80% vs. 41.67%, P<0.05), (11 (6~12) vs. 6 (6~6), P<0.001). While, in symptom group the prevalence of reflux esophagitis was marginally higher than that in control group.(P>0.05). By subgroup analysis, a significantly higher positive rate of salivary pepsin was observed in non-elderly group, compared to the elderly group. (57.14% vs 25%, P = 0.002).Furthermore, among patients in the symptom group, those with a salivary pepsin negative demonstrated higher levels of anxiety and depression compared to those with a salivary pepsin positive. (P<0.001). (Figure 1)</p><p><b><i>Conclusion:</i></b> Oral acid or burning sensation might be caused by gastroesophageal reflux. Age, together with anxiety, and depression might also be influencing factors . Salivary pepsin test might be helped to find the mechanism of oral acid or burning sensation.</p><p><b>PP-01-191</b></p><p><b>Evaluation of diagnostic test and usefulness of plain chest X-ray in esophageal achalasia</b></p><p><b>Yuto Muranami</b>, Chiaki Sato, Yusuke Taniyama, Hiroshi Okamoto, Yohei Ozawa, Hirotaka Ishida, Ryohei Ando, Yasuharu Shinozaki and Takashi Kamei</p><p><i>Tohoku University Hospital, Sendai, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Esophageal motility disorders including achalasia are sometimes difficult for diagnosis especially in early stage or lacking dilated esophagus. They decrease QOL and increase risk of esophageal cancer, so early diagnosis is important. Therefore, we evaluated the test and modality for detecting achalasia.</p><p><b><i>Material and methods:</i></b> We retrospectively reviewed 258 patients who were performed POEM at our institute between Apr 2015 and DEC 2023, and investigated their plain chest X-ray for detecting air column beside the trachea.</p><p><b><i>Results:</i></b> 120 cases were female and 138 were male. Median age at diagnosis was 44 (8 to 85). Median duration of symptom was 31.5 (0-593) months. There were 153 Chicago Classification type1,29 type2 and 19 type3 cases. 11 cases were other motility disorders. 92 cases were gradeI, 145 were gradeII and 21 were gradeIII dilation. There were 194 straight type and 64 sigmoid type cases. Diagnostic accuracy in each test were as follows. Barium radiography was 88% (22/25 cases), CT was 87% (14/16 cases), and endoscopy was 52% (57/109 cases) in clinic or hospital where they visited first. 145/254 cases (57%) could be detected by plain chest X-ray when we retrospectively reviewed. Among 52 patients who were missed by previous doctor’s endoscopy, 29 cases could be detected by X-ray.</p><p><b><i>Conclusion:</i></b> Achalasia can be easily missed by endoscopy. Plain chest X-ray is sometimes useful for detecting achalasia, so using both X-ray and endoscopy is effective especially in clinic where barium radiography or CT scanner isn’t available.</p><p><b>PP-01-192</b></p><p><b>Vonoprazan, the Answer of Unmet Needs GERD Therapy : A Systematic Review and Meta-Analysis</b></p><p><b>Mario Steffanus</b><sup>1,2</sup>, Riki Tenggara<sup>1</sup>, Angelina Yuwono<sup>1</sup>, Hasan Maulahela<sup>3</sup> and Dewa Nyoman Wibawa<sup>4</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology and Hepatology Trainee, Department of Internal Medicine, Faculty of Medicine of Universitas Indonesia, Jakarta, Indonesia;</i> <sup>3</sup><i>Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy Department of Internal Medicine Faculty of Medicine, Universitas Indonesia Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;</i> <sup>4</sup><i>Division of Gastroenterology and Hepatology, Departmen of Internal Medicine, Udayana University, Denpasar, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Proton pump inhibitors (PPIs) have traditionally been the cornerstone for treating gastroesophageal reflux disease (GERD). Despite their widespread use, several unmet needs remain, including issues related to non-erosive reflux disease, severe erosive esophagitis, post-meal heartburn, refractory GERD, atypical and extraoesophageal GERD presentations, Barrett’s oesophagus, and GERD following bariatric surgery. Vonoprazan, a potassium-competitive acid blocker, has shown promising acid-suppressing properties and has been used in GERD management. This systematic review evaluates Vonoprazan's effectiveness in addressing the unmet needs of GERD therapy.</p><p><b><i>Material and Methods:</i></b> Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines, this systematic review assesses Vonoprazan's efficacy in alleviating GERD symptoms compared to PPIs. Databases such as PubMed, ProQuest, and Google Scholar were utilized for this review. A meta-analysis was also performed to evaluate oesophageal healing.</p><p><b><i>Results:</i></b> Eighteen clinical trials, including nine randomized controlled trials (RCTs), were analyzed. Most studies indicated that Vonoprazan significantly alleviated reflux symptoms, as measured by the frequency scale for the symptoms of gastroesophageal reflux disease (FSSG) and the GERD-Questionnaire (GERD-Q) scores. Improvements were also observed in night-time symptoms (Pittsburgh Sleep Quality Score / PSQI score), mucosal healing, and gastric pH levels. Three studies were eligible for meta-analysis, demonstrating better mucosal healing in the Vonoprazan group (p Value: 0.04, OR 3.07, 95% CI 1.05-9.9).</p><p><b><i>Conclusion:</i></b> Vonoprazan is better than PPIs and shows potential for addressing the unmet needs in GERD treatment.</p><p><b><i>Keywords:</i></b> vonoprazan, reflux symptoms, effectiveness, unmet need therapy</p><p><b>PP-01-193</b></p><p><b>Association between fagerstorm test nicotine dependence and interleukin-8 with esophagitis grade in smoker’s gerd subjects</b></p><p><b>Finly Septianto</b><sup>1</sup>, Titong Sugihartono<sup>1</sup>, Budi Widodo<sup>1</sup>, Herry Purbayu<sup>1</sup>, Annisa Zahra Mufida<sup>1,2</sup> and Tri Asih Imroati<sup>2</sup></p><p><sup>1</sup><i>RSUD dr Soetomo, Surabaya, Indonesia;</i> <sup>2</sup><i>Airlangga Teaching Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Gastroesophageal Reflux Disease (GERD) is defined as a pathological condition as a result of reflux of stomach contents into the esophagus which causes various disturbing symptoms in the esophagus and extra esophagus, and one of the causes is a poor lifestyle such as smoking. This recurrent reflux condition can lead to systemic inflammation and it can be affectedby IL-8 and IL-6. Esophagitis is the most frequent complication. Elevated levels of IL-8 have been associated with disease progression, clinical severity, and severity of esophagitis in GERD patients. Aim. To determine the role of smoking severity and IL-8 levels in the development oesophagitis in smoker GERD subjects.</p><p><b><i>Material and Method:</i></b> This research is a cross-sectional analytic study at dr. Soetomo General Hospital, Surabaya. All the subjects were smokers, that consist of 28 male and 10 females. The severity of smoking was assessed by the Fagestorm Test Nicotine Dependence (FTND) questionnaire, serum IL-8 levels were measured using ELISA, and the degree of esophagitis was assessed using esophagogastroduodenoscopy (EGD). Data analysis were done using SPSS version 22.</p><p><b><i>Result:</i></b> There was a significant association between FTND and the degree of esophagitis (p = 0.037). There was no significant association between IL-8 levels and the degree of esophagitis (p = 0.697). There was no significant association between FTND and IL-8 levels (p = 0.556).</p><p><b><i>Conclusion:</i></b> There was a significant association between the severity of smoking and the degree of esophagitis in smoking GERD patients.</p><p><b>PP-01-194</b></p><p><b>Influence of Age, BMI, Gender and Bolus Viscosity on Esophageal Contractions in Healthy Individuals</b></p><p><b>Nanicha Siriwong</b>, Pakkapon Rattanachaisit, Jarongkorn Sirimongkolkasem, Tanisa Patcharatrakul and Sutep Gonlachanvit</p><p><i>Division of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study explores how bolus viscosity, age, BMI and gender modulate esophageal contractions.</p><p><b><i>Methods:</i></b> Healthy individuals without dysphagia (Swallow Disturbance Questionnaire score <1) underwent high-resolution esophageal impedance manometry (Medtronic Inc., MN, USA). Triplicate swallows of 5 mL liquid or one-tablespoon solid bolus were performed in supine and upright positions, following a random sequence based on the International Dysphagia Diet Standardization Initiative (IDDSI) levels 0-7. The Manoview software v3.0 (Medtronic Inc., MN, USA) analyzed swallowing parameter including lower esophageal sphincter (LES) integrated relaxation pressure (IRP), distal latency (DL), contractile front velocity (CFV), distal contractile integral (DCI), bolus transit time (BTT), and bolus entry time (BET). A linear mixed model assessed the effects of bolus viscosity, age, BMI and gender on swallowing metrics in both positions.</p><p><b><i>Results:</i></b> Forty-six participants (M:F 19:27, age 51±17, BMI 22.7±2.2) were enrolled. Advancing age significantly increased IRP, DCI, BTT, and BET in both positions while CFV increased only in the supine position. Thicker bolus significantly correlated with increased in DL, DCI, BTT, and BET in both positions. Higher BMI decreased IRP in both positions. (Table1). Pearson’s correlation revealed a moderate positive correlation between IRP and DCI, with correlation coefficients of 0.37 (upright) and 0.40 (supine), p < 0.001.</p><p><b><i>Conclusions:</i></b> These findings show how age, BMI, gender, bolus viscosity affect esophageal motility in healthy individuals. Older age increased IRP with stable contraction latency made swallowing thicker boluses more difficult. Further research on esophageal motility in individuals with dysphagia could enhance our understanding of these adaptations</p><p><b>PP-01-195</b></p><p><b>Effectiveness of Vonoprazan in Chinese Reflux Esophagitis Patients with Hiatal Hernia: a Real-world, Observational study</b></p><p>Yinglian Xiao<sup>1</sup>, Yiyang Dai<sup>2</sup>, Dean Tian<sup>3</sup>, Qi Song<sup>4</sup>, Li Xie<sup>4</sup> and <b>Minhu Chen</b><sup>1</sup></p><p><sup>1</sup><i>The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;</i> <sup>2</sup><i>The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu, China;</i> <sup>3</sup><i>Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;</i> <sup>4</sup><i>Takeda Pharmaceutical Company, Shanghai, China</i></p><p><b><i>Objectives:</i></b> Reflux esophagitis (RE) was closely related to hiatal hernia (HH). HH patients are more likely to have reflux symptoms like regurgitation and heartburn, with increasing acid exposure and prolonged acid clearance time. Thus, we aimed to investigate the effectiveness of vonoprazan in RE patients with HH.</p><p><b><i>Materials and Methods:</i></b> This was a subgroup analysis from VIEW (NCT04501627) study. All patients received vonoprazan 20mg orally q.d. for 4 weeks (8 weeks if insufficient benefit) with two-week safety follow-up. Symptom relief was assessed by diary of typical symptoms and GERD Questionnaire (GerdQ).</p><p><b><i>Results:</i></b> 96 RE patients with HH were evaluated, of which 79% (76/96) had HH <2cm and 21% (20/96) ≥2cm. After first one-week of treatment, the percentages of complete relief from heartburn, night-time heartburn, regurgitation, and night-time regurgitation in patients with HH ≥2cm were 30.8%, 61.5%, 46.2%, and 53.8%, while those with HH <2cm were 33.3%, 43.1%, 28.6%, and 36.0%, respectively. Higher percentages were observed in patients with HH ≥2cm with complete relief from night-time heartburn, regurgitation or night-time regurgitation. The similar trend was observed after first two-week of treatment. From baseline to week 4, percentage of patients without GERD-typical symptoms increased by 52.9% (95%CI: 28.73, 77.06), 33.4% (95%CI: 18.20, 48.53) and mean change in GerdQ score (negative values represent improvement) was -2.5 (95%CI: -3.95, -1.00), -1.6 (95%CI: -2.29, -0.83) in patients with HH ≥2cm and HH <2cm, respectively (Table 1).</p><p><b>PP-01-196</b></p><p><b>Safety of Vonoprazan in Chinese Patients: a Real-world, Observational Study</b></p><p>Yinglian Xiao<sup>1</sup>, Side Liu<sup>2</sup>, Yuping Chen<sup>3</sup>, Qi Song<sup>4</sup>, Li Xie<sup>4</sup> and <b>Minhu Chen</b><sup>1</sup></p><p><sup>1</sup><i>The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;</i> <sup>2</sup><i>Nanfang Hospital, Southern Medical University, Guangzhou, China;</i> <sup>3</sup><i>Zhuhai People's Hospital, Zhuhai, China;</i> <sup>4</sup><i>Takeda Pharmaceutical Company, Shanghai, China</i></p><p><b><i>Objectives:</i></b> The safety of vonoprazan has been demonstrated in randomized controlled trial but less commonly reported in the real-world. Thus, we investigated the safety of vonoprazan in real-world clinical practice in Chinese patients.</p><p><b><i>Materials and Methods:</i></b> VIEW study (NCT04501627) was a multi-center, single-arm, prospective, observational, real-world study, to evaluate the safety and effectiveness of vonoprazan. Patients were treated with 20 mg vonoprazan orally q.d. for 4 or 8 weeks (if the effect is insufficient), followed by a safety follow-up for 2 weeks. Total 3000 patients were enrolled, of which patients who took at least one dose of vonoprazan and provided safety information were included in this safety analysis.</p><p><b><i>Results:</i></b> Overall, 2829 patients were evaluated, of whom 87.3% (2469/2829) were non-elderly patients (aged 18-64 years) and 12.7% (360/2829) were elderly patients (aged ≥65 years). The types and frequencies of AEs were similar in the overall patients and differential age groups: 17.2% in the overall patients, 17.1% in the non-elderly patients and 18.6% in the elderly patients. The most common AEs were gastrointestinal disorders (e.g., diarrhoea, nausea), and infections and infestations (e.g., upper respiratory tract infection, gastroenteritis). The incidence of serious AEs in overall patients, non-elderly patients, and elderly patients were 1.0%, 0.8%, and 2.5%, and ADRs were 4.6%, 4.7%, and 3.9%. Most AEs and ADRs were mild or moderate.</p><p><b><i>Conclusion:</i></b> This study demonstrated that vonoprazan had a favorable safety profile in Chinese patients, with a similar safety profile in non-elderly patients and elderly patients.</p><p><b>PP-01-197</b></p><p><b>Sleep Factors and Gastroesophageal Reflux Disease: A Study on Causality, Mediation, and Pathogenesis</b></p><p><b>Ke Tao</b> and Hong Xu</p><p><i>The First Hospital of Jilin University, Changchun, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gastroesophageal reflux disease (GERD), encompassing reflux esophagitis (RE) and non-erosive reflux disease (NERD), is a prevalent chronic condition with increasing global incidence. Sleep disturbances have been linked to various diseases. This study comprehensively evaluates the relationship between multiple sleep issues and GERD, providing a novel perspective for exploring the complex causal relationships and mechanisms.</p><p><b><i>Materials and Methods:</i></b> This study utilizes data from the UK Biobank spanning from 2006 to 2010 to conduct a large-scale prospective cohort study. The impact of sleep factors on GERD incidence is examined using COX proportional hazards models, followed by Mendelian randomization to verify their causal relationships, and proteomics for intermediary analysis.</p><p><b><i>Results:</i></b> COX proportional hazards models indicate that short sleep duration, difficulty waking early, late bedtime, insomnia, snoring, daytime sleepiness, and napping all contribute to the occurrence of reflux esophagitis and non-erosive reflux disease. Mendelian randomization further confirms that insomnia promotes the occurrence of RE and NERD, short sleep duration promotes RE, and difficulty waking early promotes NERD. In proteomic intermediary analysis, we further identify 19 plasma proteins mediating NERD induced by night shifts, activating seven metabolic pathways including \"Intracellular oxygen transport,\" and 15 plasma proteins mediating RE induced by night shifts, activating two metabolic pathways including \"Nuclear receptor transcription pathway.\"</p><p><b><i>Conclusion:</i></b> Multiple sleep problems are closely associated with GERD incidence, with insomnia mediating RE and NERD through respective metabolic pathways. These findings provide new insights into GERD mechanisms and offer potential targets for the development of novel therapeutic strategies.</p><p><b>PP-01-198</b></p><p><b>Safety and long-term efficacy of hybrid-APC for treatment of Barrett’s oesophagus: An Australian pilot study</b></p><p><b>Rosalie Tripet</b></p><p><i>Royal Prince Alfred Hospital Sydney, Camperdown, Australie</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Radiofrequency ablation is gold-standard for dysplasia in Barrett's esophagus (BE), but has a stricture rate up to 15%. Hybrid-argon plasma coagulation (APC) was developed to reduce this risk. This pilot study evaluates the feasibility, tolerance, safety, and long-term efficacy of hybrid-APC for BE treatment.</p><p><b><i>Materials and methods:</i></b> Patients with confirmed BE and dysplasia were enrolled. The procedure involved elevating the mucosa with a high-pressure water jet before thermal ablation with APC. Nodular lesions were resected by EMR. Repeat procedures were performed at 3-month intervals until complete remission of dysplasia CRD and intestinal metaplasia (CRIM). Safety assessments were conducted.</p><p><b><i>Results:</i></b> Between 2017 and 2024, twenty patients (median age 68 years; 75% male) were treated with a median follow-up of over 4 years. Ten patients (56%) had a long segment of Barrett's, with low-grade dysplasia (45%), high-grade dysplasia (35%), or neoplasia (20%). Six patients (30%) required EMR. Fifteen patients (75%) achieved CRD, and twelve (67%) showed CRIM after ≤ 5 hybrid-APC treatments. Four (22%) were slow responders. There was no progression of dysplasia or cancer during the follow-up. Recurrent intestinal metaplasia (28%) or dysplasia (11%) was successfully treated with subsequent hybrid-APC sessions. No treatment-related strictures or major complications occurred.</p><p><b><i>Conclusion:</i></b> This pilot study suggests that hybrid-APC is a safe, feasible, and effective long-term treatment for dysplastic BE. Close post-treatment surveillance is needed due to recurrence risks. Some patients may require > 5 hybrid-APC treatments for remission. Larger, multicenter trials are recommended to refine patient selection and optimize the hybrid-APC technique.</p><p><b>PP-01-199</b></p><p><b>One patient, many diseases</b></p><p><b>Dmitriy Baranov</b>, Evgeny Solonitsyn, Valeria Kamalova, Evgeny Lebedev, Elena Malinovskaya, Dmitriy Chernikh and Ivan Danilov</p><p><i>V.A.Almazov National Medical Research Center (NMRC), Saint-Petersburg, Russian Federation</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Diagnosis of diseases, especially in patients with a complex comorbid background, requires increased attention, and modern technologies combined in one center improve its quality.</p><p><b><i>Case descrition:</i></b> We present the case of a 51-year-old woman with a history of Crohn's disease, primary sclerosing cholangitis. She developed jaundice and abdominal pain. First hospitalization: an increase in ALT, AST, indirect bilirubin levels, endoscopic retrograde cholangiopancreatography - stent installation in common bile duct (CBD). Мagnetic resonance imaging: hepatomegaly, fibrous changes of the pancreas. A month later, pain syndrome again, hospitalization: an increase in ALT, AST, alkaline phosphatase levels; total bilirubin – the norm). A month later, the recurrence of pain syndrome, cytolysis, and an increase in the level of total bilirubin. EUS was performed: expansion of the CBD to 9 mm, intraluminal tumor masses, due to the peculiarities of blood supply, puncture was not performed. ERCP: CBD deformity, biopsy from a tumor. Morphology: adenocarcinoma.</p><p>Colonoscopy: stenosis of the descending calving of the colon. Ulcers of the sigmoid colon; SES-CD scale - 19 points. Morphology: adenocarcinoma.</p><p>Based on the received diagnostic data, the patient was determined to be treated.</p><p><b><i>Discussion:</i></b> The use of various modern endoscopic techniques (EUS, ERCP with biopsy, high-resolution endoscopy and expert morphology) make it possible to correctly diagnose, determine the correct treatment tactics for the patient, and minimize the risk of clinical error.</p><p><b>PP-01-200</b></p><p><b>Metastatic breast cancer presenting as small bowel obstruction in adult filipino female: a case report</b></p><p><b>Jeline Symba Celocia</b></p><p><i>Baguio General Hospital And Medical Center, Bakakeng Central, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Significance:</i></b> Breast cancer is the most common cancer for both sexes in the Philippines with most cases detected at an advanced stage. Very rarely does a primary breast cancer metastasize to the GI tract, even more to small bowels, specifically the ileum. Since uncommon, this poses a diagnostic challenge to the clinicians, since GI symptoms are generally nonspecific and endoscopic findings are often variable. Thus, the patient’s rare presentation demonstrates the need to consider a metastatic breast cancer among patients with bowel obstruction.</p><p><b><i>Clinical presentation:</i></b> A 40-year-old-female with no comorbidities, presented at the ER with 1 month history of intermittent, crampy abdominal pain, aggravated by food intake.</p><p><b><i>Management:</i></b> Endoscopy and colonoscopy revealed multiple gastric ulcers, colonic polyps and internal hemorrhoids. Patient was initially treated with H.pylori triple therapy regimen with no noted relief of symptoms. Further investigation revealed radiographic evidence of small bowel obstruction. Patient underwent exploratory laparotomy and was found out to have ileal stricture at 110cm and 120cm from ileocecal valve with multiple lymphadenopathies. Biopsy of the resected ileal segment revealed an initial consideration of lymphoma. Immunohistochemistry revealed positive for CK and CK7 stains, consistent with metastatic breast cancer. Patient then was referred to Oncology service for chemotherapy and further metastatic work – up.</p><p><b><i>Recommendation:</i></b> GI symptoms are most often nonspecific in symptomatology. This should not limit a clinician with the possible differential diagnosis and should prompt the clinician to further investigate further since a GI symptom can be a presentation of an underlying metastatic disease.</p><p><b>PP-01-201</b></p><p><b>A case of stage IV pancreatic cancer who achieved good response to pembrolizumab treatment</b></p><p><b>Aya Kawanishi</b>, Yoshihiro Shirataki, Masashi Morimachi, Ayano Ito, Hiroyuki Ito, Ryuzo Deguchi and Tatehiro Kagawa</p><p><i>Department of Gastroenterology and Hepatology, Tokai University School of Medicine, Kanagawa, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> We report a case of pancreatic cancer who achieved good response to pembrolizumab treatment.</p><p><b><i>Case description:</i></b> A 62-year-old man received a CT scan examination for follow-up of an abnormal chest shadow in November 20XX. It incidentally revealed an enlarged lymph node on the lesser curvature side of the stomachHe received CT scan examinations repeatedly, which showed gradual enlargement of the gastric lesser curvature lymph node, but no primary tumors were detected. The CT scan performed in June 20XX+2 revealed a pancreatic head tumor invading the celiac artery with enlarged para-aortic lymph nodes, and the patient was referred to our hospital. We performed endoscopic ultrasound fine-needle aspiration from the lesser gastric curvature lymph node and finally made a diagnosis of stage IV pancreatic cancer. He received first-line combination therapy with gemcitabine and nab-paclitaxel. Four months later, due to the growth of the primary lesion and lymph node metastases, chemotherapy was switched to modified FOLFIRINOX. This second-line therapy was terminated 15 months later as the primary lesion had increased in size. Cancer gene panel test identified high microsatellite instability (MSI) and tumor mutation TMB 61.78 Muts/Mbp. Pebrolizumab was then started in April 20XX+3 ; six months later, the primary lesion and lymph node metastases shrank. More than 34 months have passed since the introduction of primary treatment, and treatment is ongoing.</p><p><b><i>Discussion:</i></b> This report indicates that extended prognosis is possible in pancreatic cancer by utilizing early cancer gene panel test.</p><p><b>PP-01-202</b></p><p><b>A case of fatal jejunal lymphoma manifested by massive hemtochezia</b></p><p><b>Yujin Kim</b></p><p><i>Cheju Halla General Hospital, Jeju-si 대한민국</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Lymphoma in gastrointestinal (GI) tract can present in various symptoms, from nonspecific symptoms such as dyspepsia, abdominal pain, nausea, GI bleeding, bowel perforation or obstruction.</p><p><b><i>Case Description:</i></b> A 64-year-old male patient visited emergency department room due to hematochezia one week before and dizziness. Blood tests revealed hemoglobin (Hb) level of 7g/dL. Upper GI tract was normal on endoscopy and colonoscopy showed several diverticulosis with no active bleeding. We suspected for diverticular bleeding, and hematochezia stopped after admission.</p><p>On 5th day, he passed fresh bloody stool several times, with a decrease in blood pressure of 90/56mmHg, hemoglobin 6.5g/dL, and platelet 74k/uL. Abdomen pelvis computed tomography revealed active contrast extravasation at jejunum, suggesting active bleeding. Even though emergent embolization was done, hematochezia and hemodynamic instability continued and severe anemia (Hb 5.5g/dL), ischemic liver injury, and acute kidney injury developed. We consultation for surgery and segmental jejunal resection was performed. The surgeon found multiple ulcers and variable palpable nodules.</p><p>Despite surgery, hematochezia continued and his clinical course got worse, and secondary embolization and surgery was done. But, hematochezia did not stop and he was critically ill. Despite intensive treatment, multiorgan failure progressed and he passed away on 14th day. The final pathology was proven for Extranodal NK/T cell lymphoma, nasal type.</p><p><b><i>Discussion:</i></b> We conclude that bleeding from small bowel lymphoma is rare, but it should be included in differential diagnosis in patients with massive small bowel bleeding, and aggressive management including surgery is required to define the diagnosis.</p><p><b>PP-01-203</b></p><p><b>Microsatellite instability and EOCRC in an Emerging Economy: A Study In an Indonesian Tertiary Hospital</b></p><p><b>Saskia Nursyirwan</b><sup>1</sup>, Murdani Abdullah<sup>1</sup>, Nur Rahadiani<sup>2</sup>, Susanti Susanti<sup>3,4</sup>, Mohammad Ilyas<sup>5</sup>, Virly Nanda Muzellina<sup>1</sup>, Muhammad Firhat Idrus<sup>1</sup> and Ari Fahrial Syam<sup>1</sup></p><p><sup>1</sup><i>Department of Internal Medicine Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Faculty of Medicine Universitas Indonesia / Dr. Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia;</i> <sup>2</sup><i>Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia / Dr. Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia;</i> <sup>3</sup><i>Pathgen Diagnostik Teknologi, Ir. Soekarno Science and Technology Park, National Research and Innovation Agency Republic of Indonesia, Bogor, Indonesia;</i> <sup>4</sup><i>Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah, Purwokerto, Indonesia;</i> <sup>5</sup><i>Department of Pathology, University of Nottingham, Nottingham, United Kingdom</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to explore Colorectal Cancer (CRC) Microsatellite Instability (MSI) status in Indonesian patients to better understand the role of MSI in Early-onset CRC (EOCRC) rise and role of MSI in CRC screening.</p><p><b><i>Materials and methods:</i></b> To achieve this, we retrieved fixed formalin paraffin-embedded samples and corresponding clinical data from 100 patients diagnosed with CRC between 2018 and 2020. DNA extraction was performed on the samples, and subsequent analysis utilized the N_lynch panel, real-time PCR, and HRM analysis. MSI status was determined based on the presence of two positive markers.</p><p><b><i>Results:</i></b> As much as 12.4% of our subjects were MSI-H and 34% of the subjects were early onset cases. There is a significant difference of tumor location between MSI-H and MSS, in which larger percentage of MSI-H tumor was right-sided (33.3%) than left-sided (9.7%). No significant difference was found in terms of gender, age, onset, stage, and performance status. No significant difference between gender, tumor location, stage, MSI status, and performance status between early onset and late onset group.</p><p><b><i>Conclusion:</i></b> There is a high rate of early onset CRC in Indonesia that is not caused by MSI but rather, possibly, by an undiscovered factor. Further study is needed to explore novel pathway that could explain this phenomenon.</p><p><b>PP-01-204</b></p><p><b>Characteristics of Gastrointestinal Adverse Events in Immune Checkpoint Inhibitors</b></p><p><b>Kento Ogawa</b>, Yoshihide Matsumoto and Seiji Shio</p><p><i>Division of Gastroenterology, Shinko Hospital, Kobe, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to analyze the characteristics of ICI-induced adverse events (irAEs), focusing on gastrointestinal events.</p><p><b><i>Methods:</i></b> We compared patients with gastrointestinal irAEs to those without to examine differences in cancer type, drug efficacy, and survival. We also analyzed hepatotoxicity and colitis by dividing patients into severe (grades 3 and 4) and mild (grades 1 and 2) groups.</p><p><b><i>Results:</i></b> The study included 362 patients: 38 in the irAE group and 324 in the control group. Among the 38 irAEs, there were 22 cases of liver injury, 14 of colitis (one overlapping with cholangitis), 2 of cholangitis, and 1 of elevated pancreatic enzymes. Progressive disease was more common in the control group (p=0.00774), suggesting greater treatment efficacy in patients with irAEs.</p><p>Further analysis of the 22 liver injury cases revealed 7 in the severe group and 15 in the mild group. No significant differences were found in medication types or the number of days to onset (61 vs. 48 days). Most severe cases required steroid treatment, initiated within a week, and liver injury improved in all cases.</p><p>Among colitis patients, 4 were in the severe group and 10 were in the mild group. The time to onset was longer in the severe group (p=0.0282), and their recovery time was also longer (p=0.0337). This suggests longer treatment may lead to more severe colitis. One patient in the severe group developed sepsis and died.</p><p><b><i>Conclusion:</i></b> While irAEs may indicate effective treatment, severe cases can be challenging to manage, necessitating detailed measures.</p><p><b>PP-01-205</b></p><p><b>Clinical Characteristics and Outcomes in Patients With Localized Gastric Langerhans Cell Histiocytosis: A Case Series</b></p><p>Tae-Se Kim, <b>Youngeun Oh</b>, Yang Won Min, Hyuk Lee, Byung-Hoon Min, Jun Haeng Lee, Poong-Lyul Rhee and Jae J. Kim</p><p><i>Samsung Medical Center, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Localized gastric Langerhans cell histiocytosis (LCH) characterized by abnormal proliferation of Langerhans cells in the stomach without systemic involvement, is rare; therefore, the clinical characteristics and outcomes of LCH remain unclear. We investigated the clinical characteristics and outcomes in patients diagnosed with localized gastric LCH and have also discussed treatment strategies for this rare disease.</p><p><b><i>Materials and Methods:</i></b> The study included seven patients diagnosed with localized gastric LCH at our hospital between September 1997 and December 2023. We retrospectively reviewed medical records and analyzed the clinicopathological characteristics and patient outcomes.</p><p><b><i>Results:</i></b> Endoscopically, localized gastric LCH appeared as a small erosion in the distal part of the stomach. Positron emission tomography-computed tomography revealed normal findings in 100.0% (4/4) of patients during pre-treatment workup. Immunohistochemical analysis using S-100 and CD1a showed immunopositive cells in all tested patients. Of the six patients who underwent follow-up, two (33.3%) showed metachronous recurrence at a location distinct from the initial site. However, all patients eventually showed spontaneous regression of the disease, and no gastric LCH-induced mortality was observed during follow-up.</p><p><b><i>Conclusion:</i></b> Careful and regular surveillance may be sufficient for patients with localized gastric LCH without systemic involvement.</p><p><b>PP-01-206</b></p><p><b>Endoscopic Features of Duodenal Epithelial Tumors and Histopathologic Diagnostic Capability by Immunostaining</b></p><p><b>Takuya Ohtsu</b><sup>1</sup>, Yu Takahashi<sup>1</sup>, Naohiro Nakamura<sup>1</sup>, Takuya Shijimaya<sup>1</sup>, Sanshiro Kobayashi<sup>1</sup>, Tomomitsu Tahara<sup>1</sup>, Koji Tsuta<sup>2</sup> and Makoto Naganuma<sup>1</sup></p><p><sup>1</sup><i>Kansai Medical University Gastroenterology and Hepatology, Osaka, Japan;</i> <sup>2</sup><i>Kansai Medical University Pathology and Division of Diagnostic Pathology, Osaka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The incidence of duodenal tumors is increasing partly due to improved endoscopic detection. However, preoperative diagnosis and treatment remain challenging. This study retrospectively analyzed endoscopically resected duodenal tumors, classified them based on mucosal characteristics, and evaluated marker expression for diagnostic improvement.</p><p><b><i>Materials and Methods:</i></b> We reviewed endoscopic and immunostaining findings of duodenal epithelial tumors treated at our hospital from January 7, 2006, to May 15, 2020. Location, macroscopic features, and presence of accompanying lesions were analyzed using esophagogastroduodenoscopy. Immunostaining with MUC2, MUC5AC, MUC6, and CD10 classified each mucous trait, and Ki-67 expression was examined to determine their role in distinguishing malignancy.</p><p><b><i>Results:</i></b> Fifty cases were examined. Endoscopically, a higher percentage of carcinomas were found in red lesions and duodenal bulb lesions. Immunostaining classified mucous traits into gastric, intestinal, and mixed gastrointestinal types, with 7, 23, and 20 cases, respectively. Adenocarcinoma was found in 4 (57.1%), 2 (8.7%), and 12 (60.0%) cases. Ki-67 expression was Adenocarcinoma 50%/NUMP 66.7% in gastric type, Adenocarcinoma 50%/Adenoma 71.4% in intestinal type, and Adenocarcinoma 41.7%/Adenoma 62.5% in mixed gastrointestinal type.</p><p><b><i>Conclusion:</i></b> Duodenal tumors exhibit various mucous traits, complicating diagnosis and treatment. Endoscopic findings such as location in the bulb and a red appearance were significant, with a higher percentage of malignancy in gastric-type tumors. Measuring malignancy using Ki-67 in immunostaining proved to be difficult.</p><p><b>PP-01-207</b></p><p><b>Combining Endoscopic Submucosal Dissection and Chemoradiotherapy for Management of Rectal Cancer with Deep Submucosal Invasion</b></p><p><b>Jihye Park</b>, Jae Hyun Kim, Sung Hyun Ko and Pf Seun Ja Park</p><p><i>Kosin University College of Medicine, Busan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Rectal cancer is one of the most prevalent malignancies worldwide. The introduction of Endoscopic Submucosal Dissection (ESD) offers a minimally invasive management option for early colorectal cancers. Intestinal resection after ESD is recommended in high risk groups with LNM, and chemoradiotherapy (CRT) is also effective adjuvant therapy. Herein, we report cases of patients who underwent ESD for rectal cancer and subsequently received concurrent CRT due to pathologically confirmed deep submucosal invasion.</p><p><b><i>Case Description:</i></b> Three patients came to our hospital with LST lesions located near the anal verge. The tumors were excised using ESD, and histopathological examination revealed deep submucosal invasion (depths of 1,500 to 3,900 micrometers). Two patients showed moderate tumor budding, while the third patient had no tumor budding in histopathological examination. None of the three had lymphatic, venous, or perineural invasion. Due to the deep submucosal invasion and tumor budding, further treatment was necessary, but the patients declined surgery due to concerns about anal preservation. Therefore, chemoradiotherapy for two patients and radiotherapy for third patient was administered, and follow-up CT scans and colonoscopies have shown no evidence of recurrence to date.</p><p><b><i>Discussion:</i></b> All three cases showed no evidence of recurrence on follow-up, indicating the effectiveness of combined treatment approach. This supports the potential role of CRT as adjunct to ESD in managing rectal cancer with high-risk features.</p><p>In conclusion, ESD followed by CRT or radiotherapy is a feasible and effective treatment strategy for rectal cancer with deep submucosal invasion, providing favorable oncological outcomes while preserving anal function.</p><p><b>PP-01-208</b></p><p><b>Age and Sex Differences in the Relationship of Body Weight Changes with Colon Cancer Risks</b></p><p><b>Jihye Park</b><sup>1</sup>, Pf Jae Hyun Kim<sup>1</sup>, Pf Young Ko<sup>2</sup>, Pf Hyung Jung Kim<sup>3</sup> and Pf Seun Ja Park<sup>1</sup></p><p><sup>1</sup><i>Kosin University College of Medicine, Busan, South Korea;</i> <sup>2</sup><i>Graduate School of Public Health, Korea University College of Medicine, Seoul, South Korea;</i> <sup>3</sup><i>Department of Preventive Medicine, Korea University College of Medicine, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colon cancer is a significant health concern, and obesity is a well-established risk factor. This study investigated the association between body weight changes over 10 years and the incidence of colon cancer.</p><p><b><i>Materials and Methods:</i></b> We included a healthy population that underwent a health checkup between January 2004 and December 2006 using the South Korean population database provided by the National Health Insurance Service (NHIS). We monitored them until August 2019 to identify newly developed cases of colon cancer. We categorized participants based on body weight changes and BMI. The primary outcome was the incidence of newly diagnosed colon cancer.</p><p><b><i>Results:</i></b> There was no association with body weight changes in females < 40 years, while males who exhibited a weight gain > 20% of their body weight had a higher risk for colon cancer (HR 1.65, P>z <0.001 [95% CI 1.18–2.30]). For individuals ≥ 40 years of age, males who exhibited a body weight increase of 5%–20% had an increased risk for colon cancer (HR 1.12, P>z 0.00 [95% CI 1.06–1.19]). In females, a body weight decrease > 20% was associated with a decreased risk for colon cancer (HR 0.76; P> 0.02 [95% CI 0.60–0.96]) compared to the reference group with minimal body weight change (< 5%).</p><p><b><i>Conclusion:</i></b> Our findings provide evidence that body weight changes are associated with either an increased or decreased risk for colon cancer. These findings emphasize the importance of considering body weight changes over time and tailoring prevention strategies based on age and sex.</p><p><b>PP-01-209</b></p><p><b>Comparison of the clinical profile of patients with Gallbladder cancer with and without Obstructive jaundice</b></p><p><b>Prachi Patil</b><sup>1</sup>, Akash Pawar<sup>2</sup>, Shraddha Patkar<sup>3</sup>, Mahesh Goel<sup>3</sup>, Sridhar Sundaram<sup>1</sup>, Aditya Kale<sup>1</sup> and Shaesta Mehta<sup>1</sup></p><p><sup>1</sup><i>Dept. of Digestive Diseases and Clinical Nutrition, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India;</i> <sup>2</sup><i>Clinical Research Secretariat and DAE-CTC, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India;</i> <sup>3</sup><i>Dept. of Surgical Oncology, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Patients with gallbladder cancer (GBCA) frequently present with obstructive jaundice (OJ) which is associated with advanced disease and poor outcomes in retrospective studies. We compared the clinical features of GBCA patients with and without OJ at a high-volume referral cancer center.</p><p><b><i>Materials and Methods:</i></b> 405 consecutive untreated patients with GBCA accrued over 2 years on a prospective IRB-approved study were analysed.</p><p><b><i>Results:</i></b> 37% patients with GBCA (150/405) had jaundice at presentation with biliary obstruction on imaging qualifying as OJ. The mean age was 53 years (range 24-78). Patients presenting with OJ had a higher proportion of males as compared to without OJ (45% vs. 34%, p=0.025). There was no difference in the age at presentation or socioeconomic status between those with and without OJ. A significantly higher proportion of patients with OJ had a suboptimal ECOG PS of ≥2 (28% vs. 17%, p=0.010). They also had a significantly higher proportion of anorexia (53% vs. 38%, p=0.005), weight loss (35% vs. 22%, p=0.003) and malnutrition [SGA B/C: 86% vs 57%, p=0.000, PNI <45: 98 vs. 86%, p=0.001 and serum albumin levels ≤ 3.5 g/dL: 67% vs. 20%, p=0.000] compared to those without OJ. Patients with OJ were more likely to have inoperable disease (93% vs 82%, p=0.005) and CA 19-9 > 1000 U/mL (43% vs. 26%, p=0.001) than those without.</p><p><b><i>Conclusions:</i></b> 37% of GBCA have OJ at presentation. Patients with OJ are more likely to have advanced disease and malnutrition. Survival analysis will help us rationalize treatment for these patients.</p><p><b>PP-01-210</b></p><p><b>Olfactomedin 2 Promotes the Growth and Metastasis of Colorectal Cancer</b></p><p><b>Hong Peng</b> and Jin-Jun Guo</p><p><i>Bishan Hospital of Chongqing Medical University, Chongqing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colorectal cancer (CRC) is a common malignant disease worldwide. Liver metastasis affects the survival rate of CRC patients. Understanding the mechanism governing CRC progression is thus important. Olfactomedin 2 (OLFM2) is a secretory glycoprotein belonging to the OLFM family. Although several OLFM family members show the ability to regulate CRC progression, the role of OLFM2 in CRC is still unclear.</p><p><b><i>Materials and Methods:</i></b> The expression of OLFM2 in 24 pairs of CRC and adjacent normal tissues was investigated using immunohistochemical analysis. The prognostic value of OLFM2 in CRC was studied based on publicly available data. Both in vitro and in vivo functional experiments were conducted to examine the effect of OLFM2 on CRC cell proliferation, migration, tumorigenesis, and metastasis.</p><p><b><i>Results:</i></b> Compared with relative normal tissues, CRC tissues expressed significantly greater levels of OLFM2. Increased OLFM2 expression was associated with reduced survival of CRC patients. Multivariate cox regression analysis revealed OLFM2 expression as an independent prognostic factor in CRC. Knockdown of OLFM2 inhibited the proliferation and migration of CRC cells in vitro and impaired tumorigenesis and metastasis of CRC cells in vivo. Overexpression of OLFM2 promoted an aggressive phenotype in CRC cells.</p><p><b>PP-01-211</b></p><p><b>Mechanism of GAMT (Guanidinoacetate N-Methyltransferase) Promoting Liver Metastasis in Colorectal Cancer</b></p><p><b>Hong Peng</b> and Jin-Jun Guo</p><p><i>Bishan hospital of Chongqing medical university, Chongqing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Liver metastasis is the most critical factor affecting the prognosis of colorectal cancer (CRC). In this study, we aimed to explored the mechanism of GAMT (Guanidinoacetate N-Methyltransferase) in liver metastasis of CRC.</p><p><b><i>Materials and Methods:</i></b> Expression differences and prognosis of GAMT in CRC patients were analysed in GSE49355, TCGA, and HPA databases, which were validated by immunohistochemistry. Transwell, wound healing and stem cell sphere formation assays were conducted to investigate the impact of GAMT on cell migration, invasion and stemness. A nude mouse liver metastasis model (caecum and spleen) was established to explore the effect of GAMT on CRC liver metastasis. RNA sequencing (RNAseq) was performed to explore GAMT-related mechanisms.</p><p><b><i>Results:</i></b> In GSE49355, TCGA and HPA database, upregulated GAMT was found in liver metastasis tissues comparing primary CRC, and showed a bad prognosis. Immunohistochemistry of the CRC liver metastasis tissue microarray found a similar results. In cell and nude mouse model, overexpression of GAMT enhanced the migration, invasion capabilities, stemness and liver metastasis of CRC cells, while knockdown of GAMT weakened these properties. RNAseq and western blot suggested overexpression of GAMT significantly increased the expression of C-myc and β-catenin, while knockdown of GAMT markedly decreased their expression. Wnt signaling pathway inhibitor DKK1 and β-catenin knockdown restored the effects of GAMT overexpression on cell migration and invasion capabilities in CRC cells.</p><p><b><i>Conclusion:</i></b> GAMT enhances the migratory, invasive capabilities and stemness of CRC cells by activating the Wnt/β-catenin signaling axis, and promotes liver metastasis of CRC.</p><p><b>PP-01-212</b></p><p><b>Gastric cancer chemotherapy in the very elderly (Case Series)</b></p><p><b>Masaya Sano</b>, Takashi Ueda, Hirohiko Sato, Erika Teramura, Mia Fujisawa, Ryouzou Deguchi, Masashi Matsushima and Hidekazu Suzuki</p><p><i>Tokai University School of Medicine, Isehara-city, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Reports on chemotherapy for gastric cancer in patients over 85 years old are limited. We aim to compile and report on cases of gastric cancer chemotherapy in patients over 85 years old experienced at our hospital.</p><p><b><i>Methods:</i></b> From January 1, 2015, to December 31, 2023, we extracted data on patients over 85 years old who were newly registered with gastric cancer in the electronic medical records and underwent chemotherapy.</p><p><b><i>Results:</i></b> Out of 2,218 gastric cancer patients, 141 were over 85 years old. Among them, 93 were men (66%). There were 22 surgical cases, 41 ESD cases, 3 cases of radiation therapy for bleeding control, and 10 cases of chemotherapy. 65 patients were under observation without treatment. All 10 chemotherapy cases were treated with S1 monotherapy. While it was difficult to follow up on the prognosis of all patients, we were able to track the outcomes of the 10 chemotherapy cases. No deaths were found due to serious adverse events caused by chemotherapy. However, this analysis did not demonstrate an improvement in prognosis.</p><p><b><i>Conclusion:</i></b> Depending on the strong will of the patient and family, as well as the performance status and decision-making ability, S1 monotherapy might be considered as a treatment option for very elderly gastric cancer patients over 85 years old.</p><p><b>PP-01-213</b></p><p><b>Dietary Palmitic Acid Promotes The Proliferation And Migration Of Esophageal Cancer Through Flot2</b></p><p><b>Xiao Shi</b><sup>1,3</sup>, Chen Zhang<sup>2</sup> and Ruihua Shi<sup>1,3</sup></p><p><sup>1</sup><i>Southeast university, Nanjing, China;</i> <sup>2</sup><i>Second Affiliated Hospital, Nanjing Medical University, Nanjing, China;</i> <sup>3</sup><i>Zhongda Hospital Southeast University, Nanjing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the mechanism of dietary palmitic acid (PA) on the proliferation and migration of esophageal cancer(EC).</p><p><b><i>Materials and Methods:</i></b> After the esophageal cancer cells were treated with PA for 24 hours, all the protein samples were taken for high-throughput mass spectrometry(HTMS). And then the tissue sections of patients with EC were stained by immunohistochemistry. We set up normal EC cell group, PA treatment group, and FLOT2 deficient expression cells prepared by constructing FLOT2 small interference RNA to deal with PA. The effects of PA treatment and FLOT2 deficiency on cell proliferation, cell migration and activation of downstream signal pathway were verified in vitro, and subcutaneous tumor formation in nude mice was carried out.</p><p><b><i>Results:</i></b> HTMS analysis showed that FLOT2 was up-regulated in EC. Bioinformatics analysis showed that FLOT2 expression in esophageal cancer tissues was significantly higher than that in normal tissues, and the diagnostic ROC curve showed that FLOT2 could differentiate EC to some extent. There were significant differences in FLOT2 expression of age and BMI in patients with EC. Compared with the control group, the expression levels of FLOT2, PI3K and p-AKT/AKT in PA treated group were significantly up-regulated.</p><p><b><i>Conclusion:</i></b> The high FLOT2 expression in esophageal cancer comes from the fatty acid uptake of esophageal cancer cells. The activation of PI3K/AKT signal pathway promotes the proliferation and migration of esophageal cancer cells. Interfering with FLOT2 expression can reverse this tumor promotion phenomenon.</p><p><b>PP-01-214</b></p><p><b>A retrospective study of treatment outcomes and safety for Well-differentiated grade 3 pancreatic neuroendocrine tumors</b></p><p><b>Ryusuke Shibata</b>, Shiho Arima, Koushirou Toyodome, Makoto Hinokuchi, Shirou Tanoue, Shinichi Hashimoto and Akio Ido</p><p><i>Kagoshima University, Kagoshima, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Well-differentiated grade 3 pancreatic neuroendocrine tumor (Pan-NET G3) is clinical presentation and the efficacy of therapy remain unclear. We therefore validated the clinical pathophysiology and prognosis of Pan-NET G3 in our institution.</p><p><b><i>Materials and Methods:</i></b> We retrospectively examined the clinical characteristics, treatment courses, and prognoses of patients with unresectable Pan-NET at our department between June 2017 and April 2024.</p><p><b><i>Results:</i></b> A total of 15 patients were identified. The median age was 69 (range: 35-75) years old, and 9 (60%) were men. The primary tumor site was the pancreatic head in 5 (33.3%), body/tail in 8 (53.3%), and postoperative recurrences in 2 (13.3%). Two patients had locally advanced disease, and 13 patients exhibited metastatic involvement. The metastatic sites included hepatic metastases in 12 (92.3%) patients, osseous metastasis in 1 (7.7%), and distant lymph node metastasis in 1 (7.7%). The histological diagnosis was G1/G2/G3/NEC/MINEN in 1/4/5/4/1 cases, respectively. The median Ki-67 labeling index was G1/2/G3/NEC in 2/10/40/75 patients, respectively. Fourteen patients underwent palliative first-line systemic therapy. The first-line systemic therapy was SSA in 1 patient, STZ-5FU in 8, mTOR in 1, cisplatin+etoposide in 2, irinotecan+etoposide in 1, and GEM+nab-PTX in 1. All G3 patients received STZ+5-FU therapy. The median overall survival was 23.2 (95% confidence interval [CI]: 20.0-73.4) months in G1/2, 4.8 (95% CI: 1.6-62.4) months in G3, and 5.5 (95% CI: 1.8-37.4) months in NEC.</p><p><b><i>Conclusion:</i></b> Pan-NET G3 had a poorer prognosis than NEC. Further validation in larger populations is required; however, more therapeutic options are needed.</p><p><b>PP-01-215</b></p><p><b>Significance of psoas muscle index in patients with advanced cholangiocarcinoma undergoing palliative chemotherapy</b></p><p><b>Suk Pyo Shin</b></p><p><i>Cha Bundang Medical Center, Seongnam, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aimed to determiune wheter sacropenia affects on the prognosis in patients with advanced cholangiocarcinoma undergoing palliative chemotherapy.</p><p><b><i>Materials and methods:</i></b> This study retrospectively reviewed the clinical outcomes of advanced cholangiocarcinoma patients receiving palliative chemotherapy between 2010 and 2022 that had adequate CT images. The cross sectional area of psoas muscle at L3 level was analyzed with CoreSlicer. Of the 93 patients reviewed, 47 patients that received gemcitabine, cisplatin, and Nab-paclitaxel were selected for this study.</p><p>Primary endpoint of this study was to evaluate the prognosis of patients with sarcopenia and secondary endpoint was to determine whether average PMI loss (since the first chemotherapy) during 6 month interval had any survival benefit.</p><p><b><i>Results:</i></b> The cut-off value of PMI (psoas muscle index) for sarcopenia was set to 850mm2/m2 for males and 600 for females. The study comprised 24 males and 23 females, with a median age of 66.5 years.</p><p>10 of the male patients were sarcopenic and 14 females were sarcopenic. Average PMI loss was significantly greater in non-sarcopenic patients (18.9% in males and 7.6% in females, p<0.001) than sarcopenic patients. However, non-sarcopenic patients had no significant survival benefit compared to sarcopenic patients (p=0.270 in males, p=0.665 in females). Moreover, extent of PMI loss did not seem to have significant impact on survival (p=0.270 in males, p=0.665 in females)</p><p><b><i>Conclusion:</i></b> Whether the patient had sarcopenia, as well as the average extent of PMI loss had no significant impact on overall survival of advanced cholangiocarcinoma patients undergoing palliative chemotherapy.</p><p><b>PP-01-216</b></p><p><b>Anthropometric Factors Influencing Accuracy of Preoperatory Staging in Colorectal Cancer Patients</b></p><p><b>Marsela Sina</b>, Xhensila Pemaj, Dorina Osmanaj and Skerdi Prifti</p><p><i>University Clinic Of Gastrohepatology, Dept. of Internal Disease, University of Medicine, Tirana, Albania</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Accuracy of preoperative staging for colorectal cancer (CRC), may be prone to patients’ individual characteristics. We aim to identify specific factors influencing the accuracy of preoperative TNM-staging by Computed Tomography (CT).</p><p><b><i>Methods:</i></b> Our single-center study included patients who underwent surgery for CRC, at the University Hospital Center Mother Teresa, during 2014-2017. Demographics, body measurement: body mass index (BMI), waist circumference, hips circumference, waist-to-hip ratio; and preoperative and postoperative pathologic stages, were collected for each patient. Patients were divided into accurate and mis-staging groups according to the comparison between preoperative and postoperative TNM.</p><p><b><i>Results:</i></b> 262 CRC patients (123 rectum, 139 colon), mean age 62.7 ± 11.0 years, 56.1% male; 43.9% female. Mean BMI 26.2±2.98 kg/m2; mean waist circumference 94.2±12.2cm; mean hip circumference 106.9±11.4cm and mean waist-to-hip ratio 0.9±0.1. According to WHO, 1.5% of patients were classified as underweight, 29.2% as normal weight; 58.3% as overweight, and 11% obese. 47% of patients were mis-staged of which 70.3% under-staged and 29.7% over-staged.</p><p>No significant association was found between gender (p=0.671), BMI (p=0.561), waist circumference (p = 0.299), hip circumference (p = 0.560), waist-to-hip ratio (p = 0.957), and the accurate staging.</p><p>Individuals > 50 years were significantly more likely to be mis-staged compared to under 50 (χ² = 4.488, p = 0.034).</p><p><b><i>Conclusions:</i></b> In our cohort, preoperative mis-staging occurred in 47% of cases, predominantly as under-staging. Statistical analysis revealed no association between mis-staging and BMI, waist circumference, hip circumference, and waist-to-hip ratio. Age over 50 emerged as significant predictor for CT mis-staging.</p><p><b>PP-01-217</b></p><p><b>Sex-specific roles of NRF2 and PD-L1 in right-sided colorectal cancer development: Molecular insights and implications</b></p><p><b>Chin-hee Song</b><sup>1</sup>, Nayoung Kim<sup>1,2</sup>, Yonghoon Choi<sup>1</sup>, Ryoung Hee Nam<sup>1</sup>, Soo In Choi<sup>1</sup>, Jae Young Jang<sup>1</sup>, Eun Hye Kim<sup>1</sup>, Sungchan Ha<sup>1</sup> and Ha-Na Lee<sup>3</sup></p><p><sup>1</sup><i>Departments of Internal Medicine and Research Center for Sex- and Gender-Specific Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, South Korea;</i> <sup>2</sup><i>Department of Internal Medicine and Liver Research institute, Seoul National University College of Medicine, South Korea;</i> <sup>3</sup><i>College of Pharmacy, Seoul National University, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study examined the roles of nuclear factor erythroid 2-related factor 2 (NRF2) and programmed death ligand 1 (PD-L1) in colon carcinogenesis, underscoring on sex and tumor location.</p><p><b><i>Materials and Methods:</i></b> A total of 378 participants were enrolled from Seoul National University Bundang Hospital: 88 healthy controls (HC), 139 patients with colorectal adenoma (AD), and 151 patients with colorectal cancer (CRC). During colonoscopy, the tissue samples were collected. Quantitative real-time PCR, methylation-specific PCR, and immunohistochemistry (IHC) were performed utilizing tumor samples from patients and normal mucosa in the HC group.</p><p><b><i>Results:</i></b> NRF2 mRNA expression was higher in the CRC group than in the HC and AD groups, with decreased NRF2 methylation in the AD and CRC groups. NRF2 protein expression, as evaluated by IHC, increased in the AD and CRC groups relative to that in the HC group. PD-L1 protein expression was remarkably higher in the CRC group than in the HC and AD groups. These patterns were consistent in both males and females. In sex- and CRC location-specific analyses, NRF2 protein expression was significantly higher in females, particularly in patients with right-sided CRC. Moreover, females exhibited increased PD-L1 mRNA expression compared to males in the AD group, and PD-L1 mRNA levels were higher in females with right-sided colon cancer than in those with cancer at other locations.</p><p><b><i>Conclusion:</i></b> Differences in NRF2 and PD-L1 expression indicate site-specific colon carcinogenesis based on sex, particularly in females with right-sided CRC.</p><p><b>PP-01-218</b></p><p><b>Primary Extra-Gastrointestinal Stromal Tumour (EGIST) of the retro-gastric mesentery: A case report</b></p><p><b>Duminda Subasinghe</b><sup>1</sup>, Harry Prashath<sup>2</sup>, Chamod Nirmal<sup>2</sup>, Supun Kulathunga<sup>4</sup> and Harshima Wijesinghe<sup>3</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, university Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital Of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, University of Colombo, Colombo, Sri Lanka;</i> <sup>4</sup><i>Department of Gastroenterology, The National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal stromal tumors (GISTs) are rare, accounting for only 0.1–3% of gastrointestinal malignancies, while extragastrointestinal stromal tumors (EGISTs) constitute 5% of all GISTs. Predominantly originating from Cajal-like cells, EGISTs primarily occur in the omentum, mesentery, retroperitoneum and rarely in pancreas, liver, gallbladder, urinary bladder, pleura, prostate, seminal vesicles, pelvis and vagina presenting unique diagnostic challenges. Unlike GIST they can metastasize to lymphnodes. Herein, we report a case of primary retro-gastric mesentery EGIST.</p><p><b><i>Case Description:</i></b> A 48-year-old male incidentally discovered a large intra-abdominal mass during an ultrasound scan for left sided ureteric colic. CECT revealed a large irregular vascular tumour measuring 12 x 15 x 20 cm posterior to the stomach. Intraoperatively, a tumour adherent to the stomach's posterior wall and transverse mesocolon was completely resected. He had an uneventful postoperative recovery. Histopathological examination confirmed EGIST of spindle cell type with an intermediate risk, risk stratification (AFIP) group 3b with a 12% disease progression risk.</p><p><b><i>Discussion:</i></b> EGISTs often present insidiously or with compressive symptoms, necessitating thorough diagnostic workup. While imaging aids in localization, definitive diagnosis hinges on histopathological assessment, particularly immunohistochemistry for CD117. Management revolves around surgical excision. The utility of imatinib mesylate remains uncertain. EGISTs pose diagnostic and therapeutic challenges, with surgery being the primary treatment modality. Optimal management strategies, including the role of adjuvant therapy with KIT inhibitors, require further investigation to improve outcomes and prognostication.</p><p><b>PP-01-219</b></p><p><b>Ectopic Pancreas Mimicking Gastrointestinal Stromal Tumor (GIST): A Case Report</b></p><p><b>Dedy Sudrajat</b><sup>2</sup>, Stefanus Ranty<sup>2</sup>, Jeffrey Jeffrey<sup>2</sup> and Riesye Arisanty<sup>2</sup></p><p><sup>1</sup><i>Eka Hospital Bsd, Jakarta, Indonesia;</i> <sup>2</sup><i>Grha Kedoya Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal stromal tumor (GIST) remains the most common submucosal tumor in stomach. This tumor is found mostly in gastric body. The main challenge in diagnosis of GIST remains in distinguishing it from benign lesions that do not require resection. Here we present ectopic pancreas, a submucosal tumor, mimicking as GIST lesion.</p><p><b><i>Case Description:</i></b> A 31-year-old female patient was referred with chief complaint epigastric patient and nausea for a week. No loss of appetite, vomiting, heartburn, bleeding, nor loss of weight were reported. Vital sign and laboratory test results were all within normal limit. Physical examination showed pain palpated in epigastric area. Esophagogastroduodenoscopy (EGD) was performed and found tumor located in anterior wall of greater curvature of corpus, approximately 1.5 cm in diameter. Biopsy was taken, and abdominal Computed Tomography (CT) scan showed mass of gastric wall with the size of 1.3x1.7x1.7 cm in anterior wall of greater curvature of corpus, neither lymph node or any adjacent organs were involved. Patient was then undergone laparoscopic resection, and histopathology results showed ectopic pancreas. Patient was discharged 3 days after surgery with no complaint.</p><p><b>PP-01-221</b></p><p><b>A 3-weekly TS-1 regimen as adjuvant therapy for stage II and III advanced gastric cancer</b></p><p><b>Sun Jin Sym</b><sup>1</sup>, Jihong Bae<sup>1</sup>, Dong Bok Shin<sup>1</sup>, Min-Hee Ryu<sup>2</sup>, Porf Dae Young Zang<sup>3</sup> and Woon Kee Lee<sup>4</sup></p><p><sup>1</sup><i>Gachon University Gil Medical Center, Medical Oncology, Incheon, Republic of Korea Incheon, South Korea;</i> <sup>2</sup><i>Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea;</i> <sup>3</sup><i>Division of Hematology-Oncology, Department of Internal Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea;</i> <sup>4</sup><i>Surgery, Gachon University Gil Medical Center, Incheon, Republic of Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We investigated the efficacy and safety of a 3-weekly adjuvant TS-1 regimen for 1-year in advanced gastric Cancer (AGC).</p><p><b><i>Materials and Methods:</i></b> We analyzed 93 stage II, III AGC patients who initiated a 3-weekly adjuvant TS-1 therapy from Feb 2017 to May 2022 after gastrectomy with D2 lymphadenectomy. A 3-weekly regimen consisted of TS-1 at a dosage of 80 mg/m2/day, for 2 weeks, followed by a 1-week rest until reaching a 1-year or 16 course.</p><p><b><i>Results:</i></b> In the total 93 patients, 12 patients (13%) have experienced disease recurrence during the follow-up period (median 24.6 months, range 4.2 to 63.3). We observed that 7 cases(9.5%) among the Stage II patients (n=73) were evaluated as recurrent disease, while 5 cases (25%) in the Stage III patient group(n=20) were assessed as recurrence. In Kaplan-Meier analysis, the Recurrence-Free Survival(RFS) rates at 1 year, 3 years, and 5 years for the entire patients were estimated as 92.0% (95% CI 86.5 to 97.9), 84.7% (95% CI,76.4 to 93.9), and 78.6% (95% CI, 65.8 to 94.0), respectively. 80 patients (86%) completed adjuvant 3-weekly TS-1 treatment for 1-year or 16 courses. 25 patients (26.9%) completed the treatment but required dose reduction during the entire course of therapy. Adverse events, mainly diarrhea (28%) and nausea (20%), were primarily of Grade 1 or 2.</p><p><b><i>Conclusion:</i></b> 3-weekly TS-1 regimen as adjuvant therapy exhibited good efficacy, manageable toxicity. This 3-weekly regimen as adjuvant therapy in AGC should be evaluated in future study.</p><p><b>PP-01-222</b></p><p><b>Gastric MALT Lymphoma with Disseminated Disease to Rectum: A Rare Entity</b></p><p><b>Erika Johanna Tañada-Escanlar</b> and Melchor Chan</p><p><i>UST Hospital, manila, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Mucosa-associated lymphoid tissue (MALT) lymphomas usually are confined to single extranodal organs commonly the stomach . We present a rare case of disseminated MALT lymphoma affecting the rectum and stomach presenting as painless hematochezia and rectal prolapse.</p><p><b><i>Case Presentation:</i></b> A 69-year-old Filipino male, hypertensive and diabetic complained of painless hematochezia with nontender manually reducible rectal mass. Digital rectal examination revealed a soft palpable circumferential mass. Colonoscopy exhibited multiple thickened edematous folds from 10 cm of the anal verge to the rectum. Endosonography showed a circumferential wall thickening involving all the layers of the colonic and rectal wall with perirectal lymphadenopathy. Whole Abdomen CT scan exposed a large enhancing lobulated eccentric wall thickening at the rectal region with thickened perirectal fascia with subcentimeter perirectal lymph nodes and irregularly thickened gastroesophageal junction. EGD showed a 2 cm ulcerating lesion, raised with irregular nodular border in the cardia of the stomach and negative for Helicobacter pylori infection. Histopathology from both rectal and gastric cardia revealed atypical lymphoid proliferation and Immunohistochemical Staining was positive for CD3, CD20, BCL2 and Ki-67, confirming the diagnosis of MALT Lymphoma. A multidisciplinary team was called and patient was started on Bendamustine plus Rituximab chemotherapy protocol.</p><p><b><i>Conclusion:</i></b> Notwithstanding the fact that some case reports have been published, clinicopathologic characteristics of multiorgan MALT lymphomas remain unclear. This case report highlights the importance of recognizing this rare multi-organ disease that can help elucidate optimal treatment plan.</p><p><b><i>Keywords:</i></b> case report; Disseminated MALT lymphoma, Rectal Lymphoma</p><p><b>PP-01-223</b></p><p><b>PATIENTS CHARACTERISTICS AND THE CORRELATION BETWEEN BMI TO HISTOLOGICAL GRADING OF ESOPHAGEAL CANCER</b></p><p><b>Endro Tanoyo</b></p><p><i>Sardjito Hospital, PPHI-PGI-PEGI, Sleman, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate patients characteristics, and correlation between body mass index (BMI) to histological grading of esophageal cancer in Yogyakarta population.</p><p><b><i>Methods:</i></b> Outpatients data were collected from electronic medical record of Sardjito Hospital in Yogyakarta, Indonesia, from Mei 1st 2019 to Mei 31st 2024. The study included patients over 18 years old who had proven as esophageal cancer with completed histological grading. There were 41 subjects divided into four groups based on WHO BMI categories and the histological grading divided into five criteria (well, well to moderate, moderate, moderate to poor, and poor differentiated). The data was analyzed using descriptive statistics, and a chi-square crosstabulation.</p><p><b><i>Results:</i></b> 41 patients were proven as adenocarcinoma and squamous cell carcinoma; 58,5% patients were underweight, while overweight and obese at 4,9% and 2,4%, respectively. Most of the patients were young and male at equal 65,9%. Overall percentage of Squamous cell carcinoma was 61%. In underweight group the percentage of adenocarcinoma and squamous cell carcinoma were equal (50%). The percentage of moderately differentiated in the group of underweight, overweight and obese were 37,5%, 50% and 100% respectively. The difference in histological grading between the four BMI groups was not significantly different (p = 0.862).</p><p><b><i>Conclusions:</i></b> Although there were no statistically significant differences of BMI to histological grading between the four groups, the most various histological grading were found in underweight group. Squamous cell carcinoma is the most common type in all groups and the majority are male. However, adenocarcinoma was found in 50% of underweight group.</p><p><b>PP-01-224</b></p><p><b>Clinicopathological features and prognosis of metastatic tumors in the small bowel: A large multicenter analysis</b></p><p><b>Akiyoshi Tsuboi</b><sup>1</sup>, Shiro Oka<sup>1</sup>, Takeshi Yamada<sup>2</sup>, Keigo Mitsui<sup>3</sup>, Hironori Yamamoto<sup>4</sup>, Keiichi Takahashi<sup>5</sup>, Akio Shiomi<sup>6</sup>, Kinichi Hotta<sup>7</sup>, Yoji Takeuchi<sup>8</sup>, Toshio Kuwai<sup>9</sup>, Fumio Ishida<sup>10</sup>, Shin-Ei Kudo<sup>10</sup>, Shoichi Saito<sup>11</sup>, Masashi Ueno<sup>12</sup>, Eiji Sunami<sup>13</sup>, Tomoki Yamano<sup>14</sup>, Michio Itabashi<sup>15</sup>, Kazuo Ohtsuka<sup>16</sup>, Yusuke Kinugasa<sup>17</sup>, Takayuki Matsumoto<sup>18</sup>, Tamotsu Sugai<sup>19</sup>, Toshio Uraoka<sup>20</sup>, Koichi Kurahara<sup>21</sup>, Shigeki Yamaguchi<sup>22</sup>, Tomohiro Kato<sup>23</sup>, Masazumi Okajima<sup>24</sup>, Hiroshi Kashida<sup>25</sup>, Fumihiko Fujita<sup>26</sup>, Hiroaki Ikematsu<sup>27</sup>, Masaaki Ito<sup>28</sup>, Motohiro Esaki<sup>29</sup>, Masaya Kawai<sup>30</sup>, Takashi Yao<sup>31</sup>, Madoka Hamada<sup>32</sup>, Takahiro Horimatsu<sup>33</sup>, Keiji Koda<sup>34</sup>, Yasumori Fukai<sup>35</sup>, Koji Komori<sup>36</sup>, Yusuke Saitoh<sup>37</sup>, Yukihide Kanemitsu<sup>38</sup>, Hiroyuki Takamaru<sup>39</sup>, Kazutaka Yamada<sup>40</sup>, Hiroaki Nozawa<sup>41</sup>, Tetsuji Takayama<sup>42</sup>, Kazutomo Togashi<sup>43</sup>, Eiji Shinto<sup>44</sup>, Takehiro Torisu<sup>45</sup>, Akira Toyoshima<sup>46</sup>, Naoki Ohmiya<sup>47</sup>, Takeshi Kato<sup>48</sup>, Eigo Otsuji<sup>49</sup>, Shinji Nagata<sup>50</sup>, Yojiro Hashiguchi<sup>51</sup>, Kenichi Sugihara<sup>52</sup>, Yoichi Ajioka<sup>53</sup> and Shinji Tanaka<sup>54</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Hiroshima University Hospital, Japan;</i> <sup>2</sup><i>Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Nippon Medical School, Japan;</i> <sup>3</sup><i>Department of Gastroenterology, Nippon Medical School, Graduate School of Medicine, Japan;</i> <sup>4</sup><i>Department of Medicine, Division of Gastroenterology, Jichi Medical University, Japan;</i> <sup>5</sup><i>Department of Colorectal Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Japan;</i> <sup>6</sup><i>Division of Colon and Rectal Surgery, Shizuoka Cancer Center, Japan;</i> <sup>7</sup><i>Division of Endoscopy, Shizuoka Cancer Center, Japan;</i> <sup>8</sup><i>Department of Gastrointestinal Oncology, Osaka International Cancer Institute, Japan;</i> <sup>9</sup><i>Department of Gastroenterology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Japan;</i> <sup>10</sup><i>Digestive Disease Center, Showa University Northern Yokohama Hospital, Japan;</i> <sup>11</sup><i>Department of Lower Gastrointestinal Medicine, Cancer Institute Hospital of the Japanese Foundation for Cancer Research, Japan;</i> <sup>12</sup><i>Department of Gastroenterological Surgery, Toranomon Hospital, Japan;</i> <sup>13</sup><i>Department of Surgery, Kyorin University School of Medicine, Japan;</i> <sup>14</sup><i>Division of Lower Gastrointestinal Surgery, Department of Surgery, Hyogo Medical University, Japan;</i> <sup>15</sup><i>Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Japan;</i> <sup>16</sup><i>Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Japan;</i> <sup>17</sup><i>Department of Gastrointestinal Surgery, Tokyo Medical and Dental University, Japan;</i> <sup>18</sup><i>Division of Gastroenterology, Department of Internal Medicine, Iwate Medical University, Japan;</i> <sup>19</sup><i>Department of Diagnostic Pathology, Iwate Medical University, Japan;</i> <sup>20</sup><i>Department of Gastroenterology and Hepatology, Gunma University Graduate School of Medicine, Japan;</i> <sup>21</sup><i>Division of Gastroenterology, Matsuyama Red Cross Hospital, Japan;</i> <sup>22</sup><i>Department of Gastroenterological Surgery, Saitama Medical University International Medical Center, Japan;</i> <sup>23</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Japan;</i> <sup>24</sup><i>Department of Surgery, Hiroshima City Hiroshima Citizens Hospital, Japan;</i> <sup>25</sup><i>Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, Japan;</i> <sup>26</sup><i>Department of Surgery, Kurume University School of Medicine, Japan;</i> <sup>27</sup><i>Department of Gastroenterology and Endoscopy, National Cancer Center Hospital East, Japan;</i> <sup>28</sup><i>Department of Colorectal Surgery, National Cancer Center Hospital East, Japan;</i> <sup>29</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Japan;</i> <sup>30</sup><i>Department of Coloproctological Surgery, Faculty of Medicine, Juntendo University, Japan;</i> <sup>31</sup><i>Department of Human Pathology, Juntendo University Graduate School of Medicine, Japan;</i> <sup>32</sup><i>Department of Gastrointestinal Surgery, Kansai Medical University Hospital, Japan;</i> <sup>33</sup><i>Department of Clinical Oncology, Kyoto University Hospital, Japan;</i> <sup>34</sup><i>Department of Surgery, Teikyo University Chiba Medical Center, Japan;</i> <sup>35</sup><i>Department of Gastroenterology, Maebashi Red Cross Hospital, Japan;</i> <sup>36</sup><i>Department of Gastroenterological Surgery, Aichi Cancer Center Hospital, Japan;</i> <sup>37</sup><i>Department of Gastroenterology, Asahikawa City Hospital, Japan;</i> <sup>38</sup><i>Department of Colorectal Surgery, National Cancer Center Hospital, Japan;</i> <sup>39</sup><i>Endoscopy Division, National Cancer Center Hospital, Japan;</i> <sup>40</sup><i>Department of Surgery, Coloproctology Center Takano Hospital, Japan;</i> <sup>41</sup><i>Department of Surgical Oncology, The University of Tokyo, Japan;</i> <sup>42</sup><i>Department of Gastroenterology and Oncology, Tokushima University Graduate School of Biomedical Sciences, Japan;</i> <sup>43</sup><i>Department of Coloproctology, Aizu Medical Center Fukushima Medical University, Japan;</i> <sup>44</sup><i>Department of Surgery, National Defense Medical College, Japan;</i> <sup>45</sup><i>Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Japan;</i> <sup>46</sup><i>Department of Colorectal Surgery, Japanese Red Cross Medical Center, Japan;</i> <sup>47</sup><i>Department of Advanced Endoscopy, Fujita Health University School of Medicine, Japan;</i> <sup>48</sup><i>Department of Surgery, NHO, Osaka National Hospital, Japan;</i> <sup>49</sup><i>Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Japan;</i> <sup>50</sup><i>Department of Gastroenterology, Hiroshima City North Medical Center Asa Citizens Hospital, Japan;</i> <sup>51</sup><i>Department of Surgery, Japanese Red Cross Omori Hospital, Japan;</i> <sup>52</sup><i>Tokyo Medical and Dental University, Japan;</i> <sup>53</sup><i>Division of Molecular and Diagnostic Pathology Niigata University Graduate School of Medical and Dental Sciences, Japan;</i> <sup>54</sup><i>Department of Gastroenterology, JA Onomichi General Hospital, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Owing to the rarity of metastatic tumors in the small bowel, the current understanding of their clinicopathological features and prognostic factors is limited, and we aimed to clarify these aspects in our study.</p><p><b><i>Materials and Methods:</i></b> We retrospectively examined 272 patients who were diagnosed histopathologically with metastatic tumors in the small bowel between May 2005 and August 2018 at multiple centers in Japan. We examined clinical features, such as age, sex, chief complaint, diagnosis modalities, primary site, treatment, and long-term prognosis.</p><p><b><i>Results:</i></b> In this study cohort, obstructive symptoms occurred most frequently (45% abdominal pain, 22% vomiting), and overt or occult gastrointestinal bleeding was present in 21% of patients. Enteroscopy, balloon-assisted enteroscopy, and capsule endoscopy were performed in 33%, 29%, and 13% of patients, respectively. The most common primary tumor was lung cancer (36%), followed by colorectal cancer (17%), gastric cancer (9%), and malignant melanoma (6%). Surgery (including tumor resection or bypass surgery) was performed in 78% of patients. Among the patients, the 3-year and 5-year overall survival rates were 32% and 24%, respectively. Multivariate analysis showed that surgical treatment significantly affected overall survival (Hazard ratio: 0.63, 95% Confidence Interval: 0.40–0.99, p=0.04).</p><p><b><i>Conclusion:</i></b> The lung was the most frequent primary site of metastatic tumors in the small bowel. Multivariate analysis showed that surgical treatment was a single factor that affected overall survival. Surgical treatment was associated with improved overall survival.</p><p><b>PP-01-225</b></p><p><b>A correlative analysis of molecular subtypes of gastric cancer in context to clinicopathological profiles</b></p><p><b>Kim Vaiphei</b></p><p><i>Punjab Institute Liver Biliary Sciences, Mohali, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> a comprehensive molecular analysis of gastric cancer (GC) correlating with the histological type and patient outcome.</p><p><b><i>Materials and methods:</i></b> 100 biopsy of gastric cancer analysing clinical and pathological and were assessed for MMR protein, p53 and E-cadherin by IHC; Her2/neu was assessed by IHC and FISH.</p><p><b><i>Results:</i></b> 57 - intestinal type and 43 diffuse type. Age - 25 to 90 years, mean -53.3. M:F=3.3:1. Diffuse type more common in younger female patients. 8 intestinal type were MMR deficient observed in older patients, distal tumor better outcome with wild type p53. Aberrant p53 expression in 51% associated with larger tumor and positive lymph node. E-cadherin was lost in 24 diffuse type. Her2/neu was overexpressed 15 intestinal type. Follow-up. Mean survival of patients with MMRd compared to MMR proficient were 44.8 and 22.9 months respectively. Her2/neu was associated with worst outcome. p53 and E-cadherin status did not influence patient outcome.</p><p><b><i>Conclusion:</i></b> The study brings out the importance roles of MMR protein and Her2/neu in patient selection for targeted therapy.</p><p><b>PP-01-226</b></p><p><b>Prevotella copri Enhances Colorectal Carcinogenesis via Wnt Signaling Pathway Activation</b></p><p><b>Fucheng Wang</b></p><p><i>Department Of Gastroenterology And Hepatology, General Hospital, Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to elucidate the role of Prevotella copri (P.copri), a gut microbe found in higher abundance in colorectal cancer (CRC) patients, in the development of CRC and its potential as a therapeutic target.</p><p><b><i>Materials and Methods:</i></b> We utilized colonic epithelial cell lines Caco-2 and HT-29 to assess the influence of P.copri supernatant on cell proliferation, migration, and invasion. RNA-Seq was conducted on these cells to identify gene expression changes. In vivo effects were evaluated using Apc min/+ mice, with analyses of colon tissues and fecal samples through histopathology, qPCR, western blotting, and 16S rDNA sequencing.</p><p><b><i>Results:</i></b> P.copri was confirmed to be more abundant in CRC patients by GMrepo database search and qPCR. Its supernatant increased CRC cell aggressiveness and activated the Wnt signaling pathway, as shown by RNA-Seq. Mice treated with P.copri developed more intestinal tumors, with increased tumor proliferation, reduced apoptosis, and impaired gut barrier function. The treatment also led to a shift in gut microbiota, increasing pathogenic bacteria and decreasing SCFA producers, affecting SCFA levels. Correlations between Butyricicoccus, Harryflintia, and differential gene expression were identified.</p><p><b><i>Conclusion:</i></b> P.copri drives colorectal tumorigenesis through inducing gut barrier dysfunction, the Wnt pathway activation and gut microbial dysbiosis with SCFAs-producing bacteria decreased. This confirms the close relationship between gut microbiota dysbiosis and CRC, suggesting that novel strategies targeting the gut microbiota may be effective against CRC.</p><p><b>PP-01-227</b></p><p><b>Reduced intestinal-to-diffuse conversion and immunosuppressive responses underlie superiority of neoadjuvant immunochemotherapy in gastric adenocarcinoma</b></p><p><b>Lei Wang</b><sup>1</sup>, Linghong Wan<sup>1</sup>, Xu Chen<sup>1</sup>, Peng Gao<sup>2</sup>, Zhenning Wang<sup>2</sup> and Bin Wang<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology & Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Chongqing, China;</i> <sup>2</sup><i>Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Neoadjuvant immunochemotherapy (NAIC) may lead to significant pathologic regression in locally advanced gastric adenocarcinoma (GAC), whether NAIC achieves superior benefits over neoadjuvant chemotherapy (NAC) warrants further clinical study. It is also poorly understood how the malignant cells and tumor immune microenvironment (TIME) might respond differentially to NAIC and NAC that may underlies therapeutic efficacies.</p><p><b><i>Materials and Methods:</i></b> This multi-center retrospective study included a total of 295 patients, with 67 patients receiving NAIC and 188 patients receiving NAC. Propensity score matching was utilized to analyze the neoadjuvant efficacy of the two treatment groups. Treatment-naive and paired tumor tissues after D2 gastrectomy were collected for pathological, immunological, and transcriptomic analysis.</p><p><b><i>Results:</i></b> NAIC demonstrated significantly increased rate of pathologic complete response compared to NAC (pCR: 25% vs 4%, P<0.05). Interestingly, pretreatment intestinal subtype of Lauren's classification was predictive of pathologic regression following NAIC, but not NAC. A substantial portion of cancers underwent intestinal-to-diffuse transition, which occurred less following NAIC and correlated with treatment failure. Moreover, NAIC prevented reprogramming to an immunosuppressive TIME with less active fibroblasts and exhausted CD8+ T cells, and increased numbers of mature tertiary lymphoid structures. Mechanistically, activation of the TNFα/NF-κB signaling pathway was associated with response to NAIC.</p><p><b><i>Conclusion:</i></b> NAIC is superior to NAC for locally advanced GAC, likely due to reduced intestinal-to-diffuse conversion and reprogramming to an immuno-active TIME. Modulation of the histological conversion and immunosuppressive TIME could be translatable approaches to improve neoadjuvant therapeutic efficacy.</p><p><b>PP-01-228</b></p><p><b>Intestinal-subtype as a biomarker predicting response to neoadjuvant immunochemotherapy in locally advanced gastric adenocarcinoma</b></p><p><b>Lei Wang</b>, Mengting Sun, Jinyang Li, Linghong Wan and Bin Wang</p><p><i>Department of Gastroenterology & Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Neoadjuvant immunochemotherapy (NAIC) significantly improves pathologic regression in locally advanced gastric adenocarcinoma (GAC). However, there is still a lack of effective biomarkers to stratify the beneficiary population for NAIC.</p><p><b><i>Materials and Methods:</i></b> This prospective, open-label, single-arm phase II clinical study enrolled patients to evaluate the efficacy and safety of NAIC for LAGC. To identify biomarkers for the efficacy of NAIC, we analyzed the correlation between clinical pathological characteristics and neoadjuvant efficacy, and used bulk RNA-seq of 66 paired samples before and after NAIC and scRNA-seq of 105 samples collected from 8 public databases for comprehensive analysis to explore the characteristics of the tumor immune microenvironment (TME) with different Lauren’s classifications and differences in NAIC response.</p><p><b><i>Results:</i></b> The study achieved the pre-specified primary endpoint: pCR rate was 30%, MPR rate was 43%, and safety and feasibility were met. The analysis revealed that Lauren’s classification may be a key feature influencing the efficacy of immunotherapy in gastric cancer. Further transcriptomics analysis revealed that intestinal-type gastric cancer (IGC) has a higher proportion of DNA damage repair malignant cells and CLEC9A+ DCs, and these two key cell subsets are highly correlated with efficacy of immunotherapy for gastric cancer. Intestinal.sig, constructed based on Lauren’s classification, can accurately predict the efficacy of gastric cancer immunotherapy.</p><p><b><i>Conclusion:</i></b> NAIC has good efficacy and feasibility for patients with LAGC, especially in patients with IGC. Intestinal.Sig is a novel biomarker for immunotherapy effect, which may help to discriminate patients with response to NAIC.</p><p><b>PP-01-229</b></p><p><b>Mechanism of Vitamin D Inhibiting the Development of Colon Tumors by Regulating MMP1</b></p><p><b>Lu Wang</b><sup>1</sup>, Xin Chen<sup>1</sup> and RUI Ze Xu<sup>2</sup></p><p><sup>1</sup><i>Tianjin Medical University, Tianjin, China;</i> <sup>2</sup><i>Baotou Medical University, Baotou, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To explore the relationship and mechanisms between vitamin D(VD) and colorectal tumors, offering insights into the diagnosis and treatment of colorectal cancer (CRC).</p><p><b><i>Materials and Methods:</i></b> Serum levels of 1,25(OH)2D3 were measured using the double-antibody sandwich. Bioinformatics analysis identified VD-related colorectal tumor genes, validated using the HCT116. HUB genes' changes were analyzed via RT-qPCR and Western blot.</p><p><b><i>Results:</i></b> 1. Serum levels of 1,25(OH)2D3 were 42.99±6.02μg/ml in the normal group, 37.06±9.56μg/ml in the CRA group, and 19.00±5.96μg/ml in the CRC group (p<0.05). No significant differences were found in VDR SNP among the groups (Figure 1).</p><p>2.VD-related colon cancer genes (Figure 2) exhibited significant expression differences among the groups. LASSO regression analysis identified 5 genes (Figure 3A-D). The diagnostic model constructed from these genes demonstrated high diagnostic efficiency (Figure 3E-L) and performed well in the TCGA-COAD dataset (Figure 4A-B).</p><p>3.RT-qPCR: SOSTDC1, PRKAA2, and CEACAM1 expressions decreased across the groups, while MMP1 and CCND1 expressions increased (Figure 4C).</p><p>4.In vitro experiments indicated that calcitriol inhibits the proliferation and migration of the HCT116(Figure 5), and significantly alter the expression of hub genes (Figure 6A). GSEA of MMP1 revealed a significant correlation with the JAK-STAT signaling pathway (Figure 6B). Calcitriol stimulation resulted in decreased expressions of MMP1, JAK, and STAT5 (Figure 6C).</p><p><b><i>Conclusion:</i></b> The serum of VD is significantly lower in CRC. VD inhibits the proliferation and migration of colon cancer cells, reduces oncogene expression. Therefore, VD holds significant potential in the diagnosis and treatment of colon cancer.</p><p><b>PP-01-230</b></p><p><b>Identification of Extracellular Matrix-Related Genes in the Progression of Gastric Cancer with Intestinal Metaplasia</b></p><p><b>Lu Wang</b> and Xin Chen</p><p><i>Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> By analyzing transcriptome sequencing data, molecular biomarkers that can predict the risk of gastric cancer (GC) and monitor the progression of intestinal metaplasia (IM) can be identified, providing new insights and strategies to prevent the progression of IM to GC.</p><p><b><i>Materials and Methods:</i></b> Weighted gene co-expression network analysis (WGCNA) was used to identify gene modules. Upregulated ECM-related genes were further examined using univariate Cox regression and Lasso analysis to select hub genes and construct a survival analysis model. The intestinal cell model was established by stimulating GES-1 cells with CDTA.</p><p><b><i>Results:</i></b> 1. WGCNA identified 1709 DEGs from GSE191275(Figure 1), while TCGA-STAD identified 4633 DEGs (Figure 2A). The intersection revealed 71 upregulated and 171 downregulated genes enriched in ECM-related pathways (Figure 2B-D).</p><p>2. Univariate Cox regression identified 6 genes with prognostic significance (Figure 3). Lasso regression identified SPARC/SERPINE1 as non-zero coefficient genes (Figure 4A-B). A prognostic model integrating clinical TNM staging, age, SERPINE1, and SPARC was constructed (Figure 4C-G).</p><p>3. IHC confirmed increasing SPARC protein expression across normal gastric mucosa (-), intestinal metaplasia (+-~+), and gastric cancer (+~++), with significant differences (p < 0.05) (Figure 5A-C). WB analysis showed significantly higher SPARC expression in induced intestinal cells compared to GES-1(Figure 5D).</p><p><b><i>Conclusion:</i></b> Comprehensive analysis suggests that a model incorporating clinical TNM staging, age, and SPARC/SERPINE1 expression serves as a prognostic predictor for GC. Moreover, elevated SPARC expression in IM and GC suggests its potential as a promising biomarker for early detection of GC and as a novel therapeutic target, guiding clinical applications.</p><p><b>PP-01-231</b></p><p><b>Midp619 mediates EMT to promote colorectal cancer progression by enhancing PKM2 stability</b></p><p><b>女士 Yali Wang</b>, Weilong Zhong and Wentian Liu</p><p><i>Tianjin Medical University General Hospital, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Our previous study showed that MicroRNA-derived protein (MidP619) is overexpressed in colorectal cancer (CRC) and promotes malignant progression of CRC. The aim of this study was to investigate the specific mechanism by which MidP619 promotes the malignant progression of CRC.</p><p><b><i>Materials and Methods:</i></b> MidP619 expression in human CRC and paracancerous tissues was detected using Western blotting (WB) and immunohistochemistry. The effect of MidP619 in proliferation, invasion, and migration was investigated using the CCK 8 assay, colony formation assay, transwell assay, wound healing assay, and animal investigations. RNA sequencing and WB were used to identify MidP619-regulated genes. Protein interactions with MidP619 were found using immunoprecipitation and mass spectrometry. PKM2 ubiquitination was established using cellular ubiquitination and protein stability tests. Finally, glycolysis-related indicators were assessed in CRC cells.</p><p><b><i>Results:</i></b> MidP619 was substantially expressed in CRC cells, promoting their proliferation, invasion, and migration. RNA sequencing and western blot analysis revealed that MidP619-regulated genes were involved in EMT and metabolic processes. Co-IP and immunofluorescence tests revealed that MidP619 interacted with PKM 2, affecting protein levels. PKM2 protein levels were raised in OE-MidP619 CRC cells, and they were found to be positively linked with EMT promotion. Cellular ubiquitination and protein stability experiments confirmed that this relationship improves protein stability and increases glycolysis by decreasing PKM2 ubiquitination levels.</p><p><b><i>Conclusion:</i></b> We demonstrated that MidP619/PKM2/glycolysis-induced EMT promotes CRC progression. This is the first work to reveal the functional and metabolism-related mechanisms of Midp619 in CRC, and lays the foundation for future diagnosis and targeted therapy of CRC.</p><p><b>PP-01-232</b></p><p><b>The characteristics of early gastric cancer immune microenvironment with different EBV and H.pylori infection status</b></p><p><b>Linjian Yang</b>, Yanfei Lang, Jing Ning, Weiwei Fu, Hejun Zhang, Yue Wen, Jing Zhang and Shigang Ding</p><p><i>Peking University Third Hospital, Beijing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To compare the difference of immune cells and characteristics of tertiary lymphoid structures (TLS) in the tumor microenvironment of early gastric cancer (EGC) with different Epstein-Barr virus (EBV) and Helicobacter pylori (H. pylori) infection status.</p><p><b><i>Materials and Methods:</i></b> The clinicopathological data of 247 EGC patients in Peking University Third Hospital from 2010 to 2022 were collected. By the results of EBV-encoded small RNA(EBER) in situ hybridization and H. pylori immunohistochemical staining, patients were divided into EBV and H. pylori double-positive(EBV+HP+), EBV single-positive(EBV+HP-), H. pylori single-positive(EBV-HP+) and double-negative(EBV-HP-) group. The immune cell infiltration in the mucosa was compared. 41 patients were selected to detect the tumor-infiltrating immune cells and TLS by H&E and multiplex immunofluorescence staining.</p><p><b><i>Results:</i></b> The proportion of background mucosa with massive lymphocyte infiltration in the EBV+HP- group was significantly higher than the other groups(18.2% vs 12.5% vs 0% vs 0%, p=0.015). The number of tumor-infiltrating T cells was significantly higher in the EBV+HP- group than EBV-HP+ and EBV-HP- group([1558(552.4, 2149)]vs. [440.5(353.3, 556.5)]vs. [368.7 (190.5, 543.8)]/mm², p<0.001). The density of intratumoral TLS was significantly higher in the EBV+HP- group than EBV-HP+ and EBV-HP- group [(0.5192±1.15)vs. (0.008±0.028)vs.(0.015±0.041)/mm², p=0.009]. The average distance between peritumoral TLS to the tumor was significantly greater in the EBV-HP+ group than EBV+HP- and EBV-HP- group([1.240(0.100, 4.400)]vs. [0.365(0.000, 2.145)]vs. [0.500(0.000, 1.750)]mm, p=0.008). Secondary follicle-TLS (SFL-TLS) was predominant in all groups.</p><p><b><i>Conclusion:</i></b> The immune microenvironment of EGC is dissimilar under different EBV and H. pylori infection status, demonstrating the roles of pathogens in EGC.</p><p><b>PP-01-233</b></p><p><b>Identification of macrophage-related gene signatures to explore potential molecular mechanisms in intestinal metaplasia</b></p><p><b>教授 Huiling Yu</b><sup>1,2</sup>, Kui Jiang<sup>1</sup> and Jingwen Zhao<sup>1</sup></p><p><sup>1</sup><i>Tianjin Medical University General Hospital, Tianjin, China;</i> <sup>2</sup><i>Affiliated Hospital of Hebei University, Baoding, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Macrophages are participants in chronic inflammation during Gastric intestinal metaplasia (IM). The purpose of this study was to investigate macrophage-related genes (MRGs) signatures and molecular mechanisms in IM.</p><p><b><i>Methods:</i></b> The GSE78523 dataset was used to acquire the differentially expressed genes (DEGs) between tissue samples of IM patients and normal individuals. Next, the intersection genes of DEGs and MRGs was regarded as differentially expressed MRGs (DE-MRGs). The least absolute shrinkage and selection operator and support vector machine recursive feature elimination were utilized to screen candidate genes in IM. Subsequently, the expression level and diagnostic effectiveness of the DE-MRGs were further validated via GSE60427, and nomogram was constructed based on these gene signatures. Meanwhile, enrichment analysis and immune cells infiltration analysis were accomplished. Finally, the regulatory mechanisms analysis and drug prediction of gene signatures were implemented.</p><p><b><i>Results:</i></b> APOB, SIRPA, SUCNR1 and IFNGR1 were identified as hub DE-MRGs by expression level analysis. The nomogram model had a great prediction ability of IM occurrence. GSEA enrichment analysis suggested that the hedgehog signaling pathway, ascorbate, aldarate metabolism and pentose and glucuronate interconversions were primarily involved. Furthermore, immune analysis revealed hub DE-MRGs were markedly associated with central memory CD8+ T cell, gamma delta T cell, monocyte, etc. Moreover, the qRT-PCR findings revealed a significant increase for SIRPA and IFNGR1 expression in IM samples, but SUCNR1 expression trends in the opposite direction.</p><p><b><i>Conclusion:</i></b> APOB, SIRPA, SUCNR1 and IFNGR1 were identified as hub DE-MRGs in IM, which provided some reference for further research IM in clinical practice.</p><p><b>PP-01-234</b></p><p><b>SP1-induced lncRNA ZFAS1 contributes to cell proliferation and migration in gastric cancer through AKT/mTOR signaling</b></p><p><b>Fan Zhang</b> and Ying Li and Liang He and Yu Wang</p><p><i>First Hospital Of Jilin University, Changchun City, Jilin Province, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gastric cancer remains a prevalent malignancy, with molecular drivers critical for its progression and therapeutic targeting. Recently, some malignancies have been linked to the lncRNA ZFAS1, but its mechanism of action and the reasons for its dysregulation in gastric cancer are unknown.</p><p><b><i>Materials and Methods:</i></b> We evaluated ZFAS1 expression in gastric cancer cells (SGC7901) by RT-qPCR and compared it with GES-1 cells. The LnCAR database provided insight into ZFAS1 levels in STAD compared to normal tissue. The effect of ZFAS1 knockdown on cell invasion, migration and proliferation was evaluated using transwell invasion, wound healing assays, CCK8 and flow cytometry. In addition, ZFAS1 promoter regions were examined using the JASPAR database and subsequent ChIP assays to understand SP1 transcription factor binding.</p><p><b><i>Results:</i></b> The effect of ZFAS1 on the AKT/mTOR pathway was clarified using Western blotting. SGC7901 cells were shown to have increased ZFAS1 expression, which was linked to a poor prognosis for gastric cancer. Knockdown of ZFAS1 in SGC7901 cells inhibited cell invasion, migration and proliferation and induced apoptosis. In addition, SP1 was found to upregulate ZFAS1 transcription by binding to its promoter region. ZFAS1 knockdown resulted in a significant reduction of key proteins (p-AKT, AKT, p-mTOR, and mTOR) of the AKT/mTOR pathway. When the AKT activator SC79 was introduced, the repressive effects of ZFAS1 knockdown on cell invasion, migration, proliferation, and AKT/mTOR signaling were partially reversed.</p><p><b><i>Conclusion:</i></b> Our results highlight the pivotal role of ZFAS1 in gastric cancer cell malignancy, which is modulated by the AKT/mTOR pathway.</p><p><b>PP-01-235</b></p><p><b>Amino Acid Metabolism Related Genes Model For Prognosis And Immunity In Gastric Cancer</b></p><p><b>Huan Zhang</b><sup>1</sup>, 女士 Lu Qang<sup>2</sup> and 先生 Hailong Cao<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, tianjin, China;</i> <sup>2</sup><i>Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To systematically investigate amino acid metabolic reprogramming in gastric cancer, construct prognostic models, and validate gene signatures for predictive value and clinical decision-making.</p><p><b><i>Materials and Methods:</i></b> Downloaded data; constructed amino acid metabolism prognostic models. Analyzed model genes' prognosis and clinical correlations; identified TCGA-STAD DEGs. Functional Enrichment Analysis (GO), GSEA\\GSVA enrichment analysis; PPI network and Constructed the interaction network of mRNA-miRNA, mRNA-TF and mRNA-RBP. Analyzed immune infiltration; built and correlated clinical prognostic models. Processed single cell data post-quality control; annotated cell types. Scored cells with AUCell; validated with RNA-RT-qPCR; conducted statistical analysis.</p><p><b><i>Results:</i></b> Significant negative correlations were identified between immune cells (T cells CD4 memory activated) and the expression of 8 hub genes in the TCGA-STAD dataset. Relationships between the 8 AAM-related hub genes and immune cells were analyzed using the scRNA-seq dataset GSE184198 from GC patients. Elevated expressions of the 8 hub genes in gastric cancer cells were confirmed via PCR. These genes were utilized to construct a genetic predictive model for personalized outcome prediction in gastric cancer patients.</p><p><b><i>Conclusion:</i></b> We developed an AAMRMGs prognostic model that assesses molecular signatures and the strength of infiltrative immune cells to classify AAM clusters. Risk profiles derived from hub genes were validated as independent prognostic indicators for gastric cancer, indicating a link between amino acid metabolism-related genes and patient outcomes. Cellular heterogeneity was characterized by associating hub genes with immune cells. These findings offer novel insights for survival prognostication and immunotherapy strategies in gastric cancer.</p><p><b>PP-01-236</b></p><p><b>Identification and Regulation of Exhausted T Cell Subpopulations in CRC Immune Microenvironment Using Single-Cell Genomics</b></p><p><b>博士 Luping Zhang</b> and Hong Xu and Nan Zhang and Ying Li</p><p><i>Department of Gastroenterology, The First Hospital of Jilin University, Changchun, China, Changchun, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colorectal cancer (CRC) is a major gastrointestinal malignancy in China. Over 30% of advanced CRC patients experience recurrence or metastasis post-surgery. Adjuvant immunotherapy offers hope but is hindered by T cell exhaustion. This study aims to identify and characterize exhausted T cell subpopulations in CRC using single-cell genomics and explore mechanisms to reverse T cell exhaustion and enhance immunotherapy efficacy.</p><p><b><i>Materials and Methods:</i></b> Single-cell RNA sequencing (scRNA-seq) analyzed the immune microenvironment of CRC tissues from advanced-stage patients. Exhausted T cells (Tex cells) were identified by specific surface markers and gene expression profiles. Bioinformatics analyses elucidated regulatory pathways and transcription factors involved in T cell exhaustion. Validation used flow cytometry and functional assays to assess T cell activity and cytokine production.</p><p><b><i>Results:</i></b> scRNA-seq identified Tex cell subpopulations with high expression of inhibitory receptors like PD-1, Tim-3, and Slamf6. Key transcription factors, including NFAT, Nur77, and BACH2, drove the differentiation of stem-like CD8+ Tex cell progenitors. These progenitors showed reduced antigen dependency and maintained stem-like properties through TCF-1, Eomes, and Bcl-6 expression, while antagonizing effector-related transcription factors like Blimp-1 and T-bet. Functional assays showed that targeting these pathways partially restored T cell activity and enhanced anti-tumor responses.</p><p><b><i>Conclusion:</i></b> Our study provides insights into T cell exhaustion in CRC and highlights mechanisms to reverse exhaustion. These findings could inform optimized adjuvant immunotherapy strategies, offering new avenues for effective CRC treatment.</p><p><b>PP-01-237</b></p><p><b>Biofeedback Therapy is effective in all types of Pelvic Floor Dyssynergia: A retrospective cohort study</b></p><p><b>Ayush Agarwal</b><sup>1</sup>, Abhijith Anil<sup>2</sup>, Rajkumar Bayye<sup>1</sup>, Ashish Agarwal<sup>3</sup>, Ashish Chauhan<sup>4</sup>, Saurabh Kedia<sup>1</sup>, Vineet Ahuja<sup>1</sup> and Govind Makharia<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, India;</i> <sup>2</sup><i>Department of Physiology, All India Institute of Medical Sciences, New Delhi, India;</i> <sup>3</sup><i>Department of Gastroenterology, All India Institute of Medical Sciences, Jodhpur, India;</i> <sup>4</sup><i>Department of Gastroenterology, Indira Gandhi Medical College and Hospital, Shimla, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The data on effectiveness of biofeedback therapy (BFT) among different types of Pelvic floor dyssynergia (PFD) in Indian population is limited. This study aimed to evaluate the effect of BFT on clinical and manometric parameters in PFD.</p><p><b><i>Materials and methods:</i></b> Consecutive adult patients with PFD who completed 6 sessions of anorectal manometry-based BFT at a tertiary care center in New Delhi, India were included in this retrospective cohort study. Demographic, clinical, and manometric parameters of the patients before and after BFT were evaluated. Primary outcome was the change in Cleveland clinic constipation score (CCCS), and secondary outcomes were changes in clinical symptoms and manometric parameters in different types of PFD, and baseline predictors of positive response to BFT.</p><p><b><i>Results:</i></b> Out of 240 patients who underwent BFT, 63 patients completed 6 sessions and were included: 41 (65.1%) were males with median age of 39 years (IQR 27-55 years). Type 3 (26, 41.2%) and type 1 (22, 34.9%) were most common followed by type 4 (10, 15.9%) and type 2 (5, 7.9%) PFD. There was significant improvement in CCCS after BFT (pre-therapy 7(4-8) to post-therapy 4 (2-6), p value <0.001). Improvement of ≥25% on visual analog scale in overall symptoms was observed in 47 (74.6%) patients. There was significant improvement in individual clinical symptoms and anal relaxation%. There was no significant predictor and difference in outcomes among different types of PFD according to Rao or Rome IV classification.</p><p><b><i>Conclusion:</i></b> Biofeedback therapy is effective in all types of PFD in Indian population.</p><p><b>PP-01-238</b></p><p><b>Supplementation of Lactobacillus helveticus NS8 alleviated an endogenous rat depression</b></p><p><b>Husile Alatan</b><sup>1</sup>, Shan Liang<sup>2</sup>, Yosuke Shimodaira<sup>1</sup>, Katsunori Iijima<sup>1</sup> and Feng Jin<sup>2</sup></p><p><sup>1</sup><i>Akita University, Akita City, Japan;</i> <sup>2</sup><i>MIRAI Foods Institute, Yokote-shi, Akita City, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Depressive disorder is a condition involving microbiota-gut-brain axis dysfunction. Increasing studies have attempted to improve depression through gut-microbiota regulation. However, most studies have focused on chronic stress or drug-induced exogenous depression, with only limited research on endogenous depression and gut-microbiota. This study aims to explore roles of gut-microbiota in endogenous depression, whether genetic background affects probiotic intervention, and how microbiota affects behavior.</p><p><b><i>Methods:</i></b> Wistar-Kyoto rats were used as endogenous depression models, with Wistar rats as controls. The differences in behavior, gut-microbiota, nervous system, endocrine system, immune system, and gut-barrier between two types of rats were evaluated. The effect of supplementing with Lactobacillus helveticus NS8 was studied.</p><p><b><i>Results:</i></b> Wistar-Kyoto rats exhibited abnormal gut-bacteria and mycobiota, characterized by higher bacterial α-diversity but lower fungal α-diversity, and dysfunction in the microbiota-gut-brain axis, as evidenced by a hypo-serotonergic system, hyper-noradrenergic system, defective hypothalamic-pituitary-adrenal axis, compromised gut-barrier integrity, heightened serum inflammation, and diminished gut immunity. The intervention with NS8 not only increased the fecal abundance of L. helveticus but also decreased depression-like behaviors, reduced levels of corticotropin-releasing hormone in hippocampus, increased levels of brain-derived neurotrophic factor, dopamine, and glucocorticoid receptor in the hippocampus, improved the gut-microbiota, serotonergic and noradrenergic systems.</p><p><b><i>Conclusion:</i></b> The depressive phenotype of Wistar-Kyoto rats is not only attributed to their genetic context but also closely related to their gut microbiota. Like exogenous depression, abnormal gut microbiota and dysfunctional microbiota-gut-brain axis play important roles in endogenous depression. Supplementing probiotics of NS8 is probably a promising way to improve endogenous depression.</p><p><b>PP-01-239</b></p><p><b>Improvement of Cognitive Function Following Fecal Microbiota Transplantation</b></p><p><b>Jung Hwan Lee</b>, Jongbeom Shin, Boram Cha, Ji-Taek Hong and Kye Sook Kwon</p><p><i>Inha University Hospital, Incheon, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Fecal microbiota transplantation (FMT) has emerged as a promising therapeutic intervention for various gastrointestinal and metabolic disorders. Recent studies suggest potential benefits of FMT on cognitive function, possibly through the gut-brain axis. This study aims to evaluate the impact of FMT on cognitive function in patients with cognitive impairment, using the Mini-Mental State Examination (MMSE).</p><p><b><i>Materials and Methods:</i></b> This prospective study followed 7 patients with cognitive impairment, excluding severe cases (MMSE score = 0, 6 patients). Participants underwent FMT via colonoscopy and were assessed at baseline and three months post-transplantation. Cognitive function was evaluated using the MMSE and secondary assessments including the Montreal Cognitive Assessment (MoCA).</p><p><b><i>Results:</i></b> Post-FMT, median MMSE scores changed, with the median score increasing from 12 [3-18, interquartile range] at baseline to 11 [9-16] after one week. Excluding 4 patients with no follow-up at one month, median MMSE scores of 3 patients increased from 1 [1-12] to 10 [9-18]. The MMSE score of 1 patient who followed up at 3 months increased from 23 to 28. Improvements were also observed in the median MoCA score from 0 [0-6] to 10 [9-18] after 1 month. In the microbiome study, Bifidobacterium, Ruminococcus gnavus, Collinsella, and Coprococcus genus increased after FMT.</p><p><b><i>Conclusion:</i></b> FMT appears to improve cognitive function in patients with cognitive impairment, as evidenced by increases in MMSE and MoCA scores along with changes in gut microbiota. These findings suggest that modulation of the gut microbiota may have therapeutic potential in managing cognitive decline.</p><p><b>PP-01-240</b></p><p><b>Frequency of Overlap of Functional Dyspepsia and Functional Bowel Disorders in the Clinical Setting</b></p><p><b>Shahana Parvin</b>, Karzon Dey Sarker, Rashedul Hasan, Golam Kibria and M Masudur Rahman</p><p><i>Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Overlap of disorders of gut-brain interaction (DGBI) has a negative impact on treatment outcome due to ineffective treatment approach. Overlap of these conditions is common in population-based studies, but clinical data are lacking. The aims of this study were to determine the frequency of overlap between functional dyspepsia (FD) and functional bowel disorders (FBD).</p><p><b><i>Materials and Methods:</i></b> Consecutive DGBI patients based on the ROME lV diagnostic criteria attending at the outdoor patient department in Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh were interviewed using validated Bengali ROME lV questionnaire and categorized based upon the symptom pattern as having FD, FBD or FD/FBD overlap.</p><p><b><i>Results:</i></b> Of 2,227 patients, 595 (26.72%) had DGBI [(mean age 34.81 ± 1.11 years; male 399 (67.1%)], functional esophageal, gastroduodenal, bowel disorders and anorectal disorders were present in 154 (25.9%), 359 (60.3%), 566 (95.1%) and 17 (2.9%), respectively. Most common DGBI were functional dyspepsia (FD) 267 (44.9%) followed by irritable bowel syndrome (IBS) 174 (29.2%), functional constipation (FC) 151 (25.4%) and functional diarrhea (FDr) 131 (22.0%). Overlap between FD and FBD were present in 197(33.1%). Among them, FD-IBS, FD-FC and FD-FDr overlap were present in 87(14.6%); 67(11.3%) and 54(9.1%) patients respectively.</p><p><b><i>Conclusion:</i></b> Roughly a third of patients fulfils the Rome IV criteria of DGBI. Around half of them have FD and one third of DGBI patients have FD-FBD overlap. Therefore, symptom misinterpretation may lead to treatment dissatisfaction. FD-FBD overlapping patients require multiple targeted combination treatment rather than specifically targeted treatment.</p><p><b>PP-01-241</b></p><p><b>Prophylactic treatment of cycling vomiting syndrome in children with whole food plant-based diet and probiotics</b></p><p><b>Priyanka Udawat</b><sup>1</sup> and HARSHKUMAR Patel<sup>2</sup></p><p><sup>1</sup><i>Sir H N Reliance Foundation Hospital, Mumbai, India;</i> <sup>2</sup><i>Consultant, Pediatric Neurologist, Ahmedabad, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To describe the utility of the combination of Whole Food Plant Based (WFPD) and probiotics (bifidobacterium lactis 10 billion CFU and Lactobacterium plantis 1.5 billion CFU) in the prophylactic treatment of cycling vomiting syndrome. (CVS)</p><p><b><i>Methods:</i></b> During the study period of June 2022 to march 2024; 33 children with CVS (diagnosed based on north American society paediatric gastroenterology, hepatology and nutrition criteria) studied. Acute episodes in children with CVS were managed as per standard protocols and for chronic prophylaxis they were given combination of WFPD and probiotics for 3 months duration with followup visits at 1,2,4 and 6 months of starting the treatment.</p><p><b><i>Results:</i></b> Out of 33 children 19 (57%) were boys and 14 were girls (43%) with their mean age 4.7 + 3.4 years. 18 of them (group A) were given combination of WFPD and probiotics along with standard prophylactic treatment (eg amitryptilin or topiramate) while 15(Group B) were kept only on combination of WFPD and probiotics. Both cohorts showed comparable reduction in frequency of acute episodes over six months after starting the treatment. Mean average of total episodes 1.3 + 0.4 and 1.2 + 0.2 in group A and group B respectively. (p value>0.05). Quality of life reported by parents comparable in both groups additionally in group B parents more relaxed due to they didn’t have to worry for drug related side effects.</p><p><b><i>Conclusion:</i></b> Combination of WFPD and probiotics demonstrated comparable efficacy and better tolerability compared to standard treatment as prophylactic treatment in children with CVS.</p><p><b>PP-01-242</b></p><p><b>Modulation gut brain axis with whole plant-based diet in children with autism spectrum disorder</b></p><p><b>Priyanka Udawat</b><sup>1</sup> and Harshkumar Patel<sup>2</sup></p><p><sup>1</sup><i>Sir H N Reliance Foundation Hospital, Mumbai, India;</i> <sup>2</sup><i>Consultant, Ahmedabad, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To demonstrate the efficacy and acceptability of whole food plant based diet (WFPD) as a dietary intervention in children with autism spectrum disorder aged 2-15 years.</p><p><b><i>Material/Methods:</i></b> Children were diagnosed autism spectrum disorder on the basis of DSM 5 and severity score was calculated with CARS 2 (Childhood autism rating scale). During study period of June 2023-November 2023 total 118 children were studied. Minimum 6 moths of follow up with monthly visits were obtained with CARS assessment done at 2 and 6 months visits</p><p><b><i>Results:</i></b> During study period total 158 children were enrolled but finally 118 children completed the required minimum six months of follow-up. Out of 118; 66 (56%) were boys and 52 (44%) were girls with mean age of 4.5 + 3.7 years. WFPB is made up of fruits, vegetables, whole grains legumes, nuts and seeds, and excludes animal foods. WFPB was advised and counselled to parents as per their children’s need by the team of Paediatric gastroenterologist, Lifestyle Medicine Physician & dieticians. Follow-up visits, assessment monitored by paediatric neurologist & therapists. All 118 children followed up for minimum 6 months with WFPB diet along with standard therapy plan. The improvement in CARS scores from baseline to 9.5 + 3.2 and 15.2 + 2.9 at 2 and 6 months.</p><p><b><i>Conclusion:</i></b> WFPD is simple and effective dietary intervention without the risk of nutritional compromise for the children with ASD aged 2-15 years. It shows significant improvement after implementation with increasing benefits over the use of longer duration.</p><p><b>PP-01-243</b></p><p><b>Dynamic changes in the gut microbiota after bismuth quadruple and high-dose dual therapy for H.pylorieradication</b></p><p><b>Jing Chen</b>, Yan Zhang, Hanchen Min, Junli Zhi, Shuyun Ma, Hongxia Dong, Jingshuang Yan, Xiaoyan Chi, Xiaomei Zhang and Yun-Sheng Yang</p><p><i>The First Medical Center Of The General Hospital Of The Pla, Beijing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate the impact of high-dose dual therapy(HDDT )on the gut microbiota and compare it with that of bismuth quadruple therapy (BQT).</p><p><b><i>Materials and Methods:</i></b> We enrolled outpatients (18–70 years) diagnosed with Helicobacter pylori infection by either histology or a positive 13C-urea breath test (13C-UBT) and randomly assigned to either the BQT or HDDT group. Subjects consented to provide fecal samples which were collected at baseline, Week 2, and Week 14. Amplification of the V1 and V9 regions of the 16S rRNA was conducted followed by high-throughput sequencing.</p><p><b><i>Results:</i></b> Ultimately, 78 patients (41 patients in the HDDT group and 37 in the BQT group) were enrolled in this study. Eradication therapy significantly altered the diversity of the gut microbiota. However, the alpha diversity rebounded only in the HDDT group at 12 weeks post-eradication. Immediately following eradication, the predominance of Proteobacteria, replacing commensal Firmicutes and Bacteroidetes, did not recover after 12 weeks. Species-level analysis showed that the relative abundances of Klebsiella pneumoniae and Escherichia fergusonii significantly increased in both groups at Week 2. Enterococcus faecium and Enterococcus faecalis significantly increased in the BQT group, with no significant difference observed in the HDDT group. After 12 weeks of treatment, the relative abundance of more species in the HDDT group returned to baseline levels.</p><p><b><i>Conclusion:</i></b> Eradication of H. pylori can lead to an imbalance in gut microbiota. Compared to BQT, the HDDT is a regimen with milder impact on gut microbiota.</p><p><b>PP-01-244</b></p><p><b>Diagnostic accuracy of pylori duotect (for flid and caga) in patients with upper gastrointestinal symptoms</b></p><p><b>Viet Hang Dao</b> and Phuc Binh Nguyen and Thi Thao Dang and Thi Huyen Trang Nguyen and Thu Thuong Nguyen and Thi Thu Uyen Vu and Van Long Dao</p><p><i>Hanoi Medical University, Hanoi, Viet Nam;</i> <i>Institute of Gastroenterology and Hepatology, Hanoi, Viet Nam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate diagnostic accuracy of H.pylori detection using blood test with FliD and CagA antibodies (Pylori Duotect) in Vietnamese patients with upper GI symptoms.</p><p><b><i>Methods:</i></b> A cross-sectional study was conducted at the Institute of Gastroenterology and Hepatology in Hanoi, Vietnam, focusing on adults from 18 years old with upper GI symptoms without history of treated peptic ulcers or H.pylori eradication. Participants were indicated with upper GI endoscopy and three methods to diagnose H.pylori infection: RUT, UBT, and Pylori Duotect. Patients were considered H.pylori positive if both RUT and UBT were positive. The parameters for evaluating the diagnostic accuracy of Pylori Duotect include sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV).</p><p><b><i>Results:</i></b> From December 1, 2023, to June 20, 2024, 272 patients were recruited with the mean age of 46.7 years old, 58.5% were female. The most common symptom was epigastric pain (47.4%). There were 92.7% of patients with gastritis, 12.1% with gastric ulcers, 8.1% with duodenal ulcers. 49.3% of patients were positive with Pylori Duotect test (FliD: 19.4% and CagA: 43.0%). Based on RUT and UBT results, 42.3% of patients were diagnosed with H.pylori infection. Pylori DuoTect’s sensitivity, specificity, PPV, and NPV were 80.0%, 64.7%, 50.7%, and 87.7%, respectively.</p><p><b><i>Conclusion:</i></b> The Pylori DuoTect test had low sensitivity and specificity in diagnosis H.pylori infection in Vietnamese patients with upper gastrointestinal symptoms.</p><p><b>PP-01-245</b></p><p><b>Evaluation of anxiety about gastric cancer by cancer worry scale</b></p><p><b>Sho Fukuda</b>, Kenta Watanabe, Tatsuki Yoshida, Yosuke Shimodaira, Tamotsu Matsuhashi and Katsunori Iijima</p><p><i>Akita University Graduate School of Medicine, Akita, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To elucidate the factors that influence fear and anxiety about Gastric cancer based on Helicobacter pylori infection status is the object.</p><p><b><i>Materials and Methods:</i></b> This study was conducted as a secondary evaluation of a previous study (Fukuda S, et al. Den Open. 2024;4:e329.). The survey was distributed questionnaires to participants who underwent health checkups at three facilities in Akita Prefecture between May 2021 and March 2022. The questionnaire items were Cancer Worry Scale(CWS), SF-8, and FSSG. CWS was used to assess anxiety about gastric cancer.HP infection status was ascertained from medical records.</p><p><b><i>Results:</i></b> There were 303 subjects in the study, with a median CWS of 14 (IQR:12, 17). The median CWS were 14(12, 16) in HP negative , 14(10.8, 16)in HP positive, and 15(12, 17) in HP eradicated. No statistically significant differences were found. In addition, the HP-eradicated group continued to have high anxiety levels after the eradication of HP. The logistic regression analysis results show that positive FSSGs and subnormal MCS are associated with high CWS scores.</p><p><b><i>Conclusion:</i></b> Regardless of HP infection status, many participants expressed high anxiety about GC. Anxiety levels remained particularly high in the HP-eradicated group. These results indicate problems with current age criteria-based GC screening, and appropriate patient education is needed when risk-based screening is introduced.</p><p><b>PP-01-246</b></p><p><b>Fourteen-day amoxicillin- or tetracycline-containing bismuth quadruple therapy for clarithromycin resistant Helicobacter pylori infection</b></p><p><b>Samryong Jee</b> and Cheol Woong Choi and Myeongseok Koh and Sung Eun Kim and Bong Eun Lee and Jin Lee and Ra Ri Cha</p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Due to the increase in clarithromycin resistance, the eradication rate of Helicobacter pylori is decreasing. Through this study, we compared the success rate of eradication with PAM (PPIs, amoxicillin and metronidazole) regimen and PBMT (PPIs, bismuth, metronidazole and tetracycline) regimen by adding bismuth to PAM regimen.</p><p><b><i>Materials and Methods:</i></b> This prospective multicenter study compared the eradication rates among B-PAM (bismuth 300mg four times, rabeprazole 20mg, amoxicillin 1g, and clarithromycin 500mg twice daily), PAM, and PBMT (rabeprazole 20mg twice, metronidazole 500mg three times and tetracycline 500mg with bismuth 300mg four times daily) regimens for 14 days. From December 2022 to February 2024, a total of 198 patients were enrolled at seven medical institutions in Busan, Ulsan, and Gyeongnam regions of South Korea. To diagnose the presence of clarithromycin resistance, we conducted DPO-PCR method.</p><p><b><i>Result:</i></b> A total 170 patients were included in the PP analysis. When comparing the eradication rates among the groups, the B-PAM group demonstrated a similar rate of 96.5% compared to the PBMT group (94.6%, p=0.633), while statistically significant differences were observed when compared to the PAM group (75.4%, p=0.001). The B-PAM regimen did not show significant differences in terms of side effects compared to the PAM and PBMT regimens. Additionally, symptoms of nausea and vomiting were less frequent in the B-PAM group compared to the PBMT group (p = 0.007).</p><p><b><i>Conclusion:</i></b> B-PAM regimen could be recommended as an initial treatment for Helicobacter pylori infections resistant to clarithromycin.</p><p><b>PP-01-247</b></p><p><b>Efficacy of P-cab based bismuth-containing triple therapy as the first-line eradication regimen for H.pylori infection</b></p><p><b>Dong-kyu Lee</b></p><p><i>Daejeon Eulji Medical Center, Eulji University, Daejeon, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The eradication rates of clarithromycin-containing triple regimens for Helicobacter pylori have declined. A potassium-competitive acid blocker (P-CAB) based therapy with added bismuth is expected to be effective due to its strong acid inhibition. This study compares the efficacy of a 14-day P-CAB-based bismuth-containing triple regimen (PC-BAC) with a 14-day PPI-based bismuth-containing triple regimen (P-BAC) in patients with high clarithromycin resistance.</p><p><b><i>Methods:</i></b> We retrospectively reviewed data of patients who received first-line treatment for H. pylori with either PC-BAC (50 mg tegoprazan + 300 mg bismuth subcitrate potassium + 1000 mg amoxicillin + 500 mg clarithromycin twice daily for 2 weeks) or P-BAC (40 mg pantoprazole + 300 mg bismuth subcitrate potassium + 1000 mg amoxicillin + 500 mg clarithromycin twice daily for 2 weeks). H. pylori eradication rates and adverse events were compared between the two groups.</p><p><b><i>Results:</i></b> A total of 143 patients were enrolled, including 60 patients in the PC-BAC group and 83 patients in the P-BAC group. The eradication rates were 84.7% in the PC-BAC group and 66.7% in the P-BAC group. The eradication rate was significantly higher in the PC-BAC group than in the P-BAC group (p=0.03). There was no statistical difference in the frequencies of adverse events between PC-BAC and P-BAC (p=0.38).</p><p><b>PP-01-248</b></p><p><b>Individual vs. Family-Based Screening for Helicobacter pylori Infection: A Population-Based Study</b></p><p><b>Yi-chia Lee</b><sup>1</sup>, Tsung-Hsien Chiang<sup>1</sup>, Shu-Lin Chuang<sup>1</sup>, Yi-Ru Chen<sup>1</sup>, Yu-Hsin Hsu<sup>2</sup>, Tsui-Hsia Hsu<sup>2</sup>, Pei-Chun Hsieh<sup>2</sup>, Li-Ju Lin<sup>2</sup>, Shi-Lun Wei<sup>2</sup> and Chao-Chun Wu<sup>2</sup></p><p><sup>1</sup><i>National Taiwan University Hospital, Taipei, Taiwan;</i> <sup>2</sup><i>Health Promotion Administration, Ministry of Health and Welfare, Taipei, Taiwan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Screening and treating Helicobacter pylori is an effective strategy to enhance stomach health. The family index-case method, which involves screening family members of those who test positive, may improve screening effectiveness. This study tested whether this approach also boosts screening quality indicators in a high-risk gastric cancer population.</p><p><b><i>Materials and Methods:</i></b> From September 24, 2018, to December 31, 2023, 19,151 participants from 55 indigenous Taiwanese communities underwent H. pylori testing using 13C-urea breath tests in an organized screening program. Among them, 6,825 were enrolled using the family index-case method and 12,326 through the usual method. We compared the positivity rates, refer-to-treatment rates, compliance-to-treatment rates (≥80% medication use), eradication rates, and reinfection rates between the two methods using generalized estimated equation models. Results were expressed as adjusted odds ratios (aORs) or rate ratios (RRs) with 95% confidence intervals (CIs).</p><p><b><i>Results:</i></b> The family index-case method reached 1,721 family members (143%) from 1,199 positive index cases, increasing the H. pylori detection rates (72.9% vs. 41.1%, aOR: 1.50, 95% CI: 1.43-1.56). The refer-to-treatment rates (77.3% vs. 75.1%, aOR: 1.03, 95% CI: 0.98-1.09), compliance-to-treatment rates (82.9% vs. 82.5%, aOR: 1.03, 95% CI: 0.90-1.17), and the eradication rates (78.4% vs. 81.0%, aOR: 0.98, 95% CI: 0.95-1.00) were similar between the two methods. The reinfection rate was lower in the family index-case method (0.51% vs. 1.30% per person-year, RR: 0.39, 95% CI: 0.08-1.82) than the usual screening method.</p><p><b><i>Conclusion:</i></b> The family index-case method increases the H. pylori detection rates and may reduce the reinfection rates.</p><p><b>PP-01-249</b></p><p><b>Efficacy of metronidazole-containing rescue regimens after standard triple therapy for Helicobacter pylori: A meta-analysis</b></p><p><b>Hideki Mori</b><sup>1</sup>, Toshihiro Nishizawa<sup>2</sup>, Kohei Morioka<sup>1</sup>, Motohiko Kato<sup>3</sup> and Takanori Kanai<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, International University of Health and Welfare, Narita Hospital, Narita, Japan;</i> <sup>3</sup><i>Center for Diagnostic and Therapeutic Endoscopy, Keio University School of Medicine, Tokyo, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Although standard triple therapy is still used as the first-line eradication treatment worldwide, it is unclear whether metronidazole should be included empirically in the second-line eradication treatment. The aim of this study is to compare the efficacy of metronidazole-containing regimens and metronidazole-free regimens after the failure of first-line eradication with standard triple therapy.</p><p><b><i>Materials and Methods:</i></b> PubMed, the Cochrane library, and the Igaku-Chuo-Zasshi database were searched in order to identify RCTs eligible for inclusion in the systematic review and the meta-analysis. The odds ratios (ORs) with 95% confidence intervals (CIs) were calculated.</p><p><b><i>Results:</i></b> Thirteen eligible RCTs were included. All 13 trials included a total of 2039 patients assigned to metronidazole-containing (975 patients) or metronidazole-free (1064 patients) regimens. Metronidazole-containing regimens had significantly lower eradication failure rates compared to regimens without metronidazole (OR 0.55; 95%CI 0.39–0.78). Subgroup analysis based on regional risk of metronidazole resistance demonstrated that the metronidazole-containing regimens had lower eradication failure rates not only in low-risk regions but also in high-risk regions (OR 0.29; 95%CI 0.11–0.74 and OR 0.66; 95%CI 0.49–0.91).</p><p><b><i>Conclusion:</i></b> After the failure of standard triple therapy, secondary eradication treatment regimens containing metronidazole demonstrated a higher eradication rate compared to those without metronidazole.</p><p><b>PP-01-250</b></p><p><b>Effect of macrolide exposure on tailored Helicobacter pylori eradication therapy and antibiotic resistance profiles</b></p><p><b>Jin Hee Noh</b><sup>1</sup>, Kee Don Choi<sup>2</sup>, Hee Kyong Na<sup>2</sup>, Ji Yong Ahn<sup>2</sup>, Jeong Hoon Lee<sup>2</sup>, Kee Wook Jung<sup>2</sup>, Do Hoon Kim<sup>2</sup>, Ho June Song<sup>2</sup>, Gin Hyug Lee<sup>2</sup> and Hwoon-Yong Jung<sup>2</sup></p><p><sup>1</sup><i>Hallym University Sacred Heart Hospital, Anyang, South Korea;</i> <sup>2</sup><i>Asan Medical Center, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Determining antibiotic use history accurately is challenging due to its reliance on patient recall. By determining macrolide exposure using the Korean Drug Utilization Review (DUR) system, we analyzed the impact of previous macrolide use on antibiotic resistance profiles and compared the eradication rate between tailored therapy based on macrolide exposure history and empirical therapy.</p><p><b><i>Materials and Methods:</i></b> Patients with confirmed Helicobacter pylori (H. pylori) infection who agreed to access prescription information using the Health Insurance Review and Assessment Service DUR system were enrolled between 2021 and 2023. Patients received tailored therapy, which was clarithromycin (CLR)-based triple therapy in cases without macrolide exposure and bismuth quadruple (BQ) therapy in cases with macrolide exposure. The empirical therapy group was prospectively recruited at the same time to compare the eradication rate.</p><p><b><i>Results:</i></b> A total of 418 patients (tailored therapy group, n=57; empirical therapy group, n=361) were analyzed. Among the tailored therapy group, 24.6% took macrolide antibiotics for the past 5 years. CLR resistance rates were higher in patients with previous macrolide use than in those without (66.7% vs. 7.5%, p<0.001). The tailored therapy group showed a higher eradication rate than the empirical therapy group for intention-to-treat (ITT), modified intention-to-treat (MITT), and per-protocol (PP) analyses (ITT, 86.0% vs. 75.6%; MITT, 94.2% vs. 80.3%; PP, 94.2% vs. 85.1%).</p><p><b><i>Conclusion:</i></b> Previous macrolide exposure identified using the DUR system was associated with a higher rate of CLR resistance. Tailored therapy based on macrolide exposure history led to higher eradication rates compared to empirical therapy.</p><p><b>PP-01-251</b></p><p><b>The effectiveness of Vonoprazan-based regimens for Helicobacter pylori eradication in China: a network meta-analysis</b></p><p>Cangui Zhang<sup>1</sup>, Bingyun Lu<sup>2</sup>, Li Xie<sup>3</sup>, Xi Ran<sup>3</sup> and <b>Ye Chen</b><sup>2</sup></p><p><sup>1</sup><i>Nanfang Hospital, Southern Medical University, Guangzhou, China;</i> <sup>2</sup><i>Shenzhen Hospita of Southern Medica University, Shenzhen, China;</i> <sup>3</sup><i>Takeda Pharmaceutical Company Ltd, 200126, Shanghai, China</i></p><p><b><i>Objectives:</i></b> This network meta-analysis (NMA) aims to evaluate the effectiveness of Vonoprazan (VPZ)-based regimens and their performance against proton pump inhibitor (PPI)-based treatments for Helicobacter pylori eradication, with a focus on results from the subgroup of Chinese patients.</p><p><b><i>Materials and Methods:</i></b> We included randomised controlled trials (RCT) published in PubMed, EMBASE, the Cochrane Library, web of science, CNKI, Wanfang, VIP and CBM from inception to 9th January, 2024, investigating VPZ- and PPI- based dual, triple and quadruple treatments in adult patients. Each RCT comprised at least one arm receiving VPZ-based regimens. The eradication rate of VPZ and PPI based regimens was analysed using proportional meta-analysis. NMA employing a Bayesian hierarchical random-effects model was performed. Subgroup analysis was conducted in Chinese patients using the same methods as the main analysis.</p><p><b><i>Results:</i></b> Among the 45 included studies in this review, 19 studies with 2508 patients provided information for Chinese subgroup (Figure 1). The eradication rate was 90.85% for VPZ-quadruple, 87.81% for VPZ-triple, 86.36% for VPZ-dual, 82.04% for PPI-quadruple, 78.72% for PPI-triple, and 84.38% for PPI-dual in China. In terms of surfaces under cumulative ranking curve (SUCRA), VPZ-quadruple demonstrated the potential to be the most optimal treatment (SUCRA=83.26%), followed by VPZ-triple (SUCRA=79.17%) and VPZ-dual (SUCRA=68.35%). For Chinese patients, VPZ-quadruple, triple and dual regimens showed significantly higher eradication rates than PPI-triple regimens. VPZ-quadruple and dual regimens was superior to PPI-quadruple (Table 1).</p><p><b><i>Conclusion:</i></b> In China, VPZ-based regimens demonstrated benefits on eradication compared to PPI quadruple and PPI-triple regimens, with VPZ-quadruple being the most effective.</p><p><b>PP-01-252</b></p><p><b>Effect of H. pylori eradication therapy on metabolic parameters in non-alcoholic fatty liver disease patients</b></p><p><b>Frinz Moey Caunes Rubio</b><sup>1</sup>, Cyra Kaye Cuecaco Mina<sup>1</sup>, Kevin Rigor Sese<sup>2</sup>, Jasper Ian Amparo Alindayu<sup>3</sup>, Kevin Lloyd Bedico Aboy<sup>3</sup> and Fred Lawrence Delantar Samante<sup>3</sup></p><p><sup>1</sup><i>St. Luke's Medical Center - Quezon City, Quezon City, Philippines;</i> <sup>2</sup><i>St. Luke's Medical Center - Global City, Taguig City, Philippines;</i> <sup>3</sup><i>University of the Philippines - College of Medicine, Manila City, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Recent meta-analyses confirm that Helicobacter pylori infection increases the risk of non-alcoholic fatty liver disease due to its association with insulin resistance. However, no meta-analysis has evaluated the effect of H. pylori eradication therapy on metabolic indices in non-alcoholic fatty liver disease patients. This study conducted a systematic review and meta-analysis of randomized controlled trials assessing the impact of H. pylori eradication therapy on patients with H. pylori infection and non-alcoholic fatty liver disease.</p><p><b><i>Materials and Methods:</i></b> A comprehensive search of randomized controlled trials in PubMed, Cochrane, and Google Scholar was conducted on H. pylori eradication therapy for patients with H. pylori infection and non-alcoholic fatty liver disease. Outcomes measured included triglyceride, fasting blood glucose, body mass index, total cholesterol, low-density lipoprotein, high-density lipoprotein, and homeostatic model assessment for insulin resistance. Analyses were performed with Review Manager version 5.4.</p><p><b><i>Results:</i></b> Three studies with 329 subjects were included in the meta-analysis. H. pylori eradication therapy plus standard-of-care reduced triglyceride levels by 20.97 mg/dL (95% CI: 0.01 to 41.94) compared to standard-of-care alone. Due to substantial heterogeneity (I²=69%), a random effects model was used. Other outcomes showed no significant differences between treatment and control groups.</p><p><b><i>Conclusion:</i></b> This meta-analysis indicates that adding H. pylori eradication therapy to standard-of-care significantly reduces triglyceride levels compared to standard-of-care alone. However, heterogeneity suggests effect variability across studies. The findings underscore the need for further clinical trials to evaluate the effectiveness of H. pylori eradication therapy on the metabolic parameters of patients with non-alcoholic fatty liver disease.</p><p><b>PP-01-253</b></p><p><b>Correlation Of Gastric Cancer And Helicobacter-Pylori-Positive Patients Admitted At Amai Pakpak Medical Center From 2016-2022:Retrospective-Study</b></p><p><b>Sittie Johaynee Sampaco</b> and Abdel Hussein Lucman</p><p><i>Amai Pakpak Medical Center, Marawi City, Philippines</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Gastric cancer (GC) is a major health concern worldwide and is responsible for numerous cancer-related deaths. Despite declining rates of GC in western regions in the reports of Arnold et al. In Lanao del sur, there is still no reported statistical data with regards to the association of GC and Helicobacter pylori (H. pylori) infection. Diagnostic challenges and limited access to tests exacerbate underdiagnosis. The study aimed to identify the prevalence of GC in patients admitted at APMC diagnosed with H. pylori infection and to correlate between GC in H. pylori-positive patients.</p><p>This retrospective study explored the relationship between gastric cancer and H. pylori-positive patients at APMC (2016-2022). This study reviewed the patients' medical records to unveil demographic profiles, assess correlations between gastric cancer and its associated risk factors, including H. pylori-positivity.</p><p>The results showed that there is an increasing prevalence of gastric cancer among older individuals with H. pylori infection with male predominance of about 70.4% (18 out of 27). The association between comorbidities such as hypertension, diabetes mellitus, liver cirrhosis, etc. and gastric cancer in H. pylori-positive individuals lacks substantial evidence. However, overall, there is a significant association between gastric cancer and H. pylori positivity (p-value of 0.000).</p><p>In conclusion, this study showed that H. pylori infection is an independent risk factor for gastric cancer. Furthermore, the prevalence rate of gastric cancer among H. Pylori positive patients is 8.11% emphasizing the need for comprehensive care strategies and further studies to refine prevention approaches and elucidate demographic associations.</p><p><b>PP-01-254</b></p><p><b>Ulcer outside and inside - Effective strategies of managing Crohn’s disease associated Pyoderma Gangrenosum</b></p><p><b>Lok Ka Lam</b></p><p><i>Queen Mary Hospital, Hong Kong</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pyoderma gangrenosum (PG) is an uncommon inflammatory ulcerative skin lesion that is frequently associated with inflammatory bowel disease (IBD). We aim to discuss the stepwise approach on the management of PG related to Crohn’s disease (CD).</p><p><b><i>Case Description:</i></b> We report a classical case of CD-associated PG in a 75-year-old woman with persistent leg ulcer that was refractory to conventional steroids, cyclosporin A, azathioprine and infliximab. She was then administered adalimumab and her ulcer healed after four months of treatment. The clinical presentation, investigation modality, and choice of treatment throughout her one-year disease course is highlighted in Figure1.</p><p><b><i>Discussion:</i></b> Our case has demonstrated a good response to adalimumab in which objective clinical improvement could be seen at two months and complete healing at four months. Adalimumab has few advantages. First, adalimumab is administered subcutaneously and could be taken at home. Second, there were less allergic reactions as adalimumab is a fully human anti-TNF-α antibody.</p><p>We have proposed a management algorithm for CD-associated PG (Figure2). In mild disease, therapy consists of local wound care and topicals. For more severe disease, systemic agents are necessary. These include initial induction with prednisolone, together with steroid-sparing agents such as azathioprine. The second line treatment will be infliximab. If refractory, adalimumab can be considered. Whether newer biologics, such as ustekinumab or small molecules, are effective will need further data to support. Before choosing a therapy, the following should be taken into consideration: medical history, presence of concomitant systemic disease, severity of ulcers, and disease course.</p><p><b>PP-01-255</b></p><p><b>Correlation between SCFA, Fecal Calprotectin, Systemic Inflammation and CD4 Level in HIV Patients on ARV</b></p><p><b>Randy Adiwinata</b><sup>1,2</sup>, Agung Nugroho<sup>3</sup>, PN Harijanto<sup>3</sup>, Bradley Jimmy Waleleng<sup>4</sup>, Fandy Gosal<sup>4</sup>, Luciana Rotty<sup>4</sup>, Jeanne Winarta<sup>4</sup>, Andrew Waleleng<sup>4</sup>, Marcellus Simadibrata<sup>5</sup> and Erni Juwita Nelwan<sup>6</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>2</sup><i>Gastrointestinal Cancer Center, MRCCC Siloam Hospital Semanggi, Jakarta, Indonesia;</i> <sup>3</sup><i>Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>4</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>5</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia;</i> <sup>6</sup><i>Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To find the correlation between gut short chain fatty acid (SCFA), fecal calprotectin (FC), high-sensitivity C-reactive Protein (hsCRP), and CD4+ cell count in HIV patients on antiretroviral (ARV) and undetectable viral load.</p><p><b><i>Materials and Methods:</i></b> This observational analytical-study was conducted at Prof. Dr. R. D. Kandou Hospital. We included HIV patients receiving first-line ARVs (tenofovir-lamivudine-efavirenz) regularly for at least one year and had undetectable viral load in past 6 months. Patients were evaluated for SCFA, fecal calprotectin level, hsCRP, and CD4+. Spearman correlation test was used to assess the correlation between variables.</p><p><b><i>Results:</i></b> Twenty-five patients were included, 65% were male, with median age 33 (IQR 27.5-40.75) years, median ARV consumption duration 14 (12.75-18) months, median SCFA 13 (IQR 9.25-15) μmol/g, FC 16.45 (IQR 9.33-24.43) ug/g, hsCRP 1.6 (IQR 0.93-2.65) mg/L, and CD4+ 603.5 (IQR 443-792.75) cells/uL. Correlation test showed significant negative correlation between FC levels and CD4+ (r=-0.553;p=0.011), significant positive correlation between FC levels and hsCRP (r=0.448;p=0.047), and significant negative correlation between hsCRP levels and CD4+ (r=-0.540;p=0.014). No significant correlation between SCFA with other variables.</p><p><b><i>Conclusion:</i></b> Decreased gut inflammation may lead to lower persistent systemic inflammation and higher CD4+ level in HIV patients on ARV, while gut inflammation may not be mediated by SCFA level.</p><p><b>PP-01-256</b></p><p><b>Extremely high prevalence of multidrug-resistant organisms in gut flora of hospitalised patients with gastro-intestinal illness</b></p><p>Vineet Ahuja<sup>1</sup>, <b>Himanshu Narang</b><sup>1</sup>, Purva Mathur<sup>2</sup>, Saurabh Kedia<sup>1</sup>, Govind Makharia<sup>1</sup>, Aparna Ningombam<sup>2</sup>, Arti Kapil<sup>3</sup>, Anoop Saraya<sup>1</sup>, Pramod Garg<sup>1</sup>, Shalimar<sup>1</sup>, Deepak Gunjan<sup>1</sup>, Soumya Jagannath Mahapatra<sup>1</sup>, Anshuman Elhence<sup>1</sup>, Samagra Agarwal<sup>1</sup>, Manjeet Kumar Goyal<sup>1</sup>, Malambo Mubbunu<sup>1</sup>, Bipul Kumar<sup>1</sup>, Mukesh Singh<sup>1</sup>, Mamta Puraswani<sup>2</sup>, M Nizam Ahmed<sup>2</sup>, Ashita Kumari<sup>2</sup>, Mahak Verma<sup>1</sup> and Radha Tiwari<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, India;</i> <sup>2</sup><i>Department of Laboratory Medicine, Trauma Centre, AIIMS, New Delhi, India;</i> <sup>3</sup><i>Department of Microbiology, All India Institute Of Medical Sciences (AIIMS), New Delhi, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To find out prevalence of multidrug-resistant organism(MDRO) colonisation in hospitalised patients, and its concordance with MDR infections and mortality</p><p><b><i>Methods:</i></b> We conducted a cross-sectional study at our tertiary-care centre in India. Rectal swab for bacterial culture was obtained from all consecutive patients admitted in our Gastroenterology ward and ICU over 2-month period (October-November 2022). Isolates were assessed for antibiotic sensitivity using VITEK 2 compact(BioMérieux Inc.). Organism was labelled “multi-drug resistant” based on non-susceptibility to any agent in at least 3 antimicrobial classes. Polymerase chain reaction (PCR) for antimicrobial resistance(AMR) genes was done using PCR system(Bio-RadTM).</p><p><b><i>Results:</i></b> One hundred-seven patients with chronic liver disease and its complications (41.1%), acute pancreatitis(27.1%), inflammatory bowel disease(7.5%), acute cholangitis(6.5%) and other gastrointestinal disorders(17.8%) were included. Mean hospitalisation duration was 14+13 days, with 59.2%, 52.5% and 22.5% requiring central venous catheterization, Foley catheterization and mechanical ventilation, respectively. 106(99.1%) samples were positive for MDRO, predominantly E.coli(75.7%), Klebsiella pneumoniae(16.8%) and Enterococcus fecium(9.3%). NDM(New-Delhi-metallo-β-lactamase, carbapenemase)(64.5%) was most prevalent AMR gene, followed by TEM(extended-spectrum-beta-lactamase,ESBL)(58.9%) and OXA(carbapenemase)(55.1%). 15% of other clinical samples were positive for MDRO, most common pathogen being K.pneumoniae(50%). Concordance for MDRO was highest in acute pancreatitis (16.7% for E.coli and 33.3% for K.pneumoniae). Isolated gut MDR colonization was associated with 22%(n=20) mortality, while patients with MDRO in both stool and another clinical sample had 50%(n=8) mortality.</p><p><b><i>Conclusions:</i></b> We report extremely high prevalence of gut colonisation with MDR organisms in hospitalised patients with GI illness. Urgent policy measures are needed to address the imminent threat of MDR infections.</p><p><b>PP-01-257</b></p><p><b>Kale improves chronic constipation through changes in the gut microbiome</b></p><p><b>Hirotada Akiho</b><sup>1</sup>, Mitsuru Esaki<sup>2</sup> and Eikichi Ihara<sup>3</sup></p><p><sup>1</sup><i>Kitakyushu Municipal Medical Center, Kitakyushu, Japan;</i> <sup>2</sup><i>Mayo Clinic Arizona, Phoenix, USA;</i> <sup>3</sup><i>Kyushu University Hospital, Fukuoka, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Kale, known for its high fiber and isothiocyanates content, is anticipated to enhance bowel movement (BM) both directly and by improving the intestinal environment. We assessed the effects of Kale consumption on BM and gut microbiome in patients with mild Chronic Constipation (CC).</p><p><b><i>Materials and Methods:</i></b> This was a prospective single-arm exploratory study. Fifteen patients with mild CC aged 50-90 were enrolled in this study. Each patient consumed a beverage containing 30 g of Kale twice a day for up to five weeks. Stool and colonic mucosa were collected at baseline and post-intervention (4 weeks after the start of Kale intervention) for 16S rRNA amplicon sequencing and metabolomic analysis. Patients recorded their daily BM. The study outcomes included the changes in BM frequency, gut microbiome and metabolome between the baseline and post-intervention.</p><p><b><i>Results:</i></b> Median age of patients was 69 years. The overall Kale consumption rate was 94%. Significant improvement was observed in days with BM (+0.5/w, p<0.05), frequency of Spontaneous BM (+1.0/w, p<0.05) and Bristol Stool Scale (+1.0/w, p<0.001). Pairwise PERMANOVA test confirmed that stool microbiota was significantly different between patients with CC and healthy volunteers (p<0.05). Firmicutes to Bacteroidota ratio and Campylobacteria decreased in the mucosal microbiota (p<0.05). The concentration of fecal acetic acid significantly increased at post-intervention, and this was positively associated with the number of days with BM (P=0.063).</p><p><b><i>Conclusion:</i></b> Kale induced improvement of BM in patients with mild CC, which may result from the changing gut microbiome and increasing acetic acid.</p><p><b>PP-01-258</b></p><p><b>Risk of LGI Bleeding in NSAID and Proton Pump Inhibitor Users Compared with NSAID-only Users</b></p><p><b>Jae Myung Cha</b> and Moonhyung Lee</p><p><i>Kyung Hee University Hospital At Gangdong, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Recent studies have indicated an increased risk of lower gastrointestinal bleeding with the combined use of non-steroidal anti-inflammatory drugs (NSAIDs) and proton pump inhibitors (PPIs). We analyzed the comparative the risk of lower gastrointestinal bleeding between NSAID + PPI users and NSAID-only users.</p><p><b><i>Materials and Methods:</i></b> This retrospective, observational study analyzed data from five hospitals using a common data model to investigate the risk of lower gastrointestinal bleeding between NSAID + PPI users (target cohort) and NSAID-only users (comparative cohort). Cox proportional hazard models and Kaplan–Meier estimations were employed after extensive propensity score matching.</p><p><b><i>Results:</i></b> Among 24,530 and 57,264 individuals in the target and comparative cohorts, respectively, 8,728 propensity score-matched pairs were analyzed. The risk of lower gastrointestinal bleeding was significantly higher in the NSAID + PPI users than in the NSAID-only users (hazard ratio = 2.843, 95% confidence interval = 1.998–4.044; p < 0.001). Similar findings were also noted in elderly patients > 65 years (hazard ratio = 2.737), males (hazard ratio = 2.963), and females (hazard ratio = 3.221). However, the risk of lower gastrointestinal bleeding was comparable between NSAID + mucoprotective agent users and the NSAID-only users (hazard ratio = 2.057, 95% confidence interval = 0.714–5.924; p = 0.172).</p><p><b><i>Conclusion:</i></b> The risk of lower gastrointestinal bleeding was higher in the NSAID + PPI users than the NSAID-only users. However, the risk of lower gastrointestinal bleeding was comparable between NSAID + mucoprotective agent users and the NSAID-only users.</p><p><b>PP-01-259</b></p><p><b>Bacterial D-ribose inhibits gastrointestinal motility through PAR2-p38 pathway in functional constipation</b></p><p><b>Nian Chen</b> and Liping Guo and Bangmao Wang and Lu Zhou</p><p><i>Tianjin Medical University General Hospital, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The role of gut microbial metabolites has received increasing attention in gastrointestinal diseases. However, little is known about gut microbial metabolites in function constipation (FC). This study was designed to investigate the potential effects of bacterial metabolites on FC.</p><p><b><i>Materials and Methods:</i></b> To identify differential metabolites between FC patients and controls, serum and stool samples were subjected to untargeted metabolomics. To confirm the candidate metabolites affecting the phenotypes of FC, these differential metabolites were perfused in intestinal segments. Further, the anti-diarrhea effects of metabolites were verified in senna leaf-induced diarrhea model.</p><p><b><i>Results:</i></b> We identified 74 metabolites which were significantly up-regulated in serum or feces samples between FC patients and controls. Among these metabolites, D-ribose was detected in both serum and feces and displayed an apparent inhibition of the amplitude and frequency of spontaneous contraction in vitro perfusion of isolated intestinal segments of both humans and mice. In addition, we isolated Lactococcus formosensis (Lf) from the liver and MLN of mice which received fecal microbiota transplantation (FMT) from FC patients. The significantly differential metabolites D-ribose was also up-regulated in Lf supernatant. Oral administration of D-ribose inhibited the expression of PAR2-p38 and contributed to constipation. What’s more, in senna leaf-induced diarrhea model, D-ribose down-regulated the expression of PAR2-p38 and performed a distinct anti-diarrhea effect.</p><p><b><i>Conclusion:</i></b> D-ribose may cause disorder of gastrointestinal motility and contribute to the development of FC. Our study illustrated the involvement of D-ribose in FC, which provided a new target for the understanding and treating of FC.</p><p><b>PP-01-260</b></p><p><b>Clinical characteristics and outcomes of immunecheckpoint inhibitor related colitis: a single center retrospective cohort study</b></p><p>Jing Chen and <b>Guanzhou Zhou</b> and Junzhe Chen and Fei Pan</p><p><i>The First Medical Center Of The General Hospital Of The Pla, Beijing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Immune checkpoint inhibitors-related colitis (IRC) is the main reason of discontinue of Immune checkpoint inhibitors (ICIs). The clinical and endoscope characteristics, treatment methods and outcomes of IRC are not well understood. This study describes the above issues and compare the difference between patients having short or long duration from last ICI use to the onset of colitis.</p><p><b><i>Methods:</i></b> This study retrospectively enrolled IRC patients from December 2021 to October 2023 diagnosed in one hospital. Based on the duration from last ICI use to the onset of colitis, patients were divided into short group (<2 weeks) and long group (>2 weeks). Clinical and endoscope characteristics were compared.</p><p><b><i>Results:</i></b> Totally, 16 males and 4 females were included in study (Figure 1). 11 patients underwent colonoscopy and the Mayo scoring system showed a score of 3 in nine (82.0%) patients, 1 and 2 in one (9.0%) patient respectively. 10 patients received hormone therapy, 1 patient received infliximab treatment, 3 patients received mesalazine combined with probiotics, and 6 patients received symptomatic antidiarrheal treatment. All patients received hormone or infliximab therapy responded well. Patients with short duration from last ICI treatment to the colitis occurrence have lower total protein and albumin level, indicating a worse nourishment.</p><p><b><i>Conclusion:</i></b> Clinically, patients with IRC mostly present with diarrhea and they usually have ulcer on the endoscopy with entire colon involvement. Patients with short duration from last ICI treatment to the colitis occurrence indicate a worse nourishment. During clinical treatment, attention should be paid to providing nutritional support.</p><p><b>PP-01-261</b></p><p><b>Study on the Mechanism of Lactobacillus oris BE7N in Improving Lipid Metabolism Disorders</b></p><p><b>Yi Chen</b> and Gang Sun</p><p><i>Department Of Gastroenterology And Hepatology, First Medical Center, Chinese Pla General Hospital, China, Beijing, 中国</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The gut microbiota plays a critical role in obesity's pathogenesis, making probiotic therapies a promising future approach. This study investigates the effects and mechanisms of Lactobacillus oris BE7N (LOB7N), isolated from centenarians' fecal samples in Hainan, on lipid metabolism disorders using the db/db obese mouse model.</p><p><b><i>Materials and Methods:</i></b> Four-week-old male db/db (C57BL/KsJ) mice were divided into control and treatment groups. The treatment group received LOBE7N bacterial powder by gavage (2x10^8 cfu/mouse/day, 200 μl), while the control group received the equivalent volume of lyophilized powder solution. Glucose tolerance and cold tolerance tests were conducted. Feces were collected for 16S rRNA sequencing. LC-MS/MS-based untargeted metabolomics analyzed secondary metabolites in LOBE7N supernatants with anti-obesity effects.</p><p><b><i>Results:</i></b> LOBE7N treatment significantly reduced body weight, epididymal fat, and liver weight in db/db mice. It improved insulin resistance and cold tolerance. Serum TC and LDL-C levels were significantly decreased. HE staining showed it reduced liver fat deposition and improved browning of brown adipose tissue. RT-PCR results indicated it upregulated thermogenic genes and increased fatty acid oxidation genes in skeletal muscle. 16S rRNA sequencing revealed increased cecal microbiota diversity and higher abundance of short-chain fatty acid-producing bacteria. LC-MS/MS-based untargeted metabolomics showed that it secretes lipid-lowering docosapentaenoic acid (DPA) and gut barrier-enhancing indole-3-propionic acid (IPA). In vitro studies indicated these metabolites enhance fatty acid oxidation in muscle cells.</p><p><b><i>Conclusion:</i></b> Lactobacillus oris BE7N, may improve lipid metabolism disorders in obese mice by promoting fatty acid oxidation in skeletal muscle through its metabolites, DPA and IPA.</p><p><b>PP-01-262</b></p><p><b>Animal experimental study on theterahertz spectral characteristics of acute mesenteric ischemia tissue</b></p><p><b>Yi Chen</b>, Yujia Jing, Jun Chen and Gang Sun</p><p><i>Department Of Gastroenterology And Hepatology, First Medical Center, Chinese Pla General Hospital, Beijing, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Acute mesenteric ischemia (AMI) is associated with high mortality and disability rates, yet early-stage diagnosis remains challenging due to the absence of sensitive and reliable diagnostic methods. Terahertz waves, an emerging non-destructive testing technology, have gained increasing application in the field of biology.</p><p><b><i>Materials and Methods:</i></b> In this study, an acute mesenteric ischemia (AMI) model was induced in six Bama miniature pigs through ligation of the mesenteric vessels. Terahertz transmission spectroscopy, spanning the range of 6000-30 cm<sup>-1</sup>, was conducted at intervals of 0, 30, 60, 120, 240, and 300 minutes post-ligation. To assess mesenteric changes pre- and post-ischemia, gross observation, endoscopic evaluation, histopathological analysis, and terahertz spectral analysis were utilized, with pathological sections serving as the gold standard. The terahertz spectra were then analyzed to assess the ischemic alterations within the mesenteric tissues.</p><p><b><i>Results:</i></b> Terahertz transmission spectroscopy was conducted on tissues affected by acute intestinal ischemia; however, no novel characteristic peaks were identified in the resulting spectra. Nonetheless, the absorption coefficient curve displayed an initial increase followed by a subsequent decrease, with the peak absorption coefficient occurring 30 minutes post-ligation. Notably, the most significant change, as indicated by the analysis of the characteristic peak height, was observed in the PO<sub>2</sub><sup>-</sup> symmetric stretch, corresponding to a wavenumber of 1084 cm<sup>-1</sup>.</p><p><b><i>Conclusion:</i></b> Terahertz transmission spectroscopy is useful in the identification of AMI.</p><p><b>PP-01-263</b></p><p><b>Elobixibat versus Senna in the treatment of chronic constipation in who failed relief with laxatives</b></p><p>Piyapat Chungsamanukool</p><p><i>Bhumibol Adulyadej Hospital, Thailand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>This study was a single-center, randomized, double-blinded controlled study at Bhumibol Adulyadej Hospital from March 1, 2023, until February 29, 2024. Patients who were diagnosed diabetic with functional constipation who have previously failed relief with laxatives. We were randomized to 10 mg elobixibat and 15 mg senna once daily for twelve weeks.</p><p><b><i>Result:</i></b> 42 patients were randomized and included in the complete analysis set. In the Elobixibat groups for 12 weeks, the increased frequency of spontaneous bowel movements was significantly higher than baseline 2.67 times per week compared with 1.19 times per week in the senna group (P = <0.001) and a significant increase than baseline Bristol stool scale 2.14 compared to the senna group, which exhibited a Bristol stool scale 1.29 (P = 0.001). The levels of HDL were significantly increased after administration of elobixibat than senna (8.90 mg/dL and -3.57 mg/dL, respectively; P =0.02)</p><p><b>PP-01-264</b></p><p><b>Accuracy Systemic Inflammation Index, Systemic Inflammatory cell ratios, CEA Distinguishing Malignant or Benign in Colonoscopy</b></p><p><b>Nelila Fitriani</b></p><p><i>Sriwijaya University, Palembang, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to evaluate the accuracy of the Systemic Inflammation Index (SII), Systemic Inflammatory Cell Ratios (SICR), and Carcinoembryonic Antigen (CEA) in distinguishing malignant colorectal cancer (CRC) from benign lesions in patients undergoing diagnostic colonoscopy.</p><p><b><i>Materials and Methods:</i></b> A retrospective analysis was conducted on 141 patients who underwent diagnostic colonoscopy at Mohammad Hoesin General Hospital from January 2024 to juni 2024. Patients were categorized into two groups based on histopathological findings: malignant (n=71) and benign (n=70) lesions. Serum levels of SII, SICR, and CEA were measured pre-procedure. Statistical analysis, including receiver operating characteristic (ROC) curves, was performed to determine the diagnostic performance of each biomarker.</p><p><b><i>Results:</i></b> The mean SII, SICR, and CEA levels were significantly higher in the malignant group compared to the benign group (p<0.001). ROC analysis showed that SII had an area under the curve (AUC) of 0.76, SICR had an AUC of 0.54, and CEA had an AUC of 0.84.</p><p><b><i>Conclusion:</i></b> CEA remains a robust biomarker for distinguishing malignant from benign colorectal lesions. Further prospective studies are warranted to validate these findings and refine diagnostic algorithms for CRC.</p><p><b><i>Keywords:</i></b> Systemic Inflammation Index, Systemic Inflammatory Cell Ratios, Carcinoembryonic Antigen, Diagnostic Accuracy</p><p><b>PP-01-265</b></p><p><b>Dietary emulsifier-induced-lipotoxicity promotes intestinal aging.</b></p><p><b>Linlin He</b> and hailong Cao</p><p><i>Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gut barrier function deteriorates with age, which plays a key role in intestinal permeability and protecting gut health.Excess emulsifier P80 intake can disrupt the intestinal barrier, negatively affecting host health.We aimed to investigate the effects of P80 intake on intestinal aging of senescence-accelerated mice.</p><p><b><i>Materials and Methods:</i></b> Senescence-accelerated mouse prone-8 mice (SAMP8) mice were randomly divided into two groups, one group was given PBS as the Control group, and the other group was given P80 as the experimental group for three months.The senescence-associated secretory phenotype(SASP), mucosal barrier function, and oxidative stress molecules were assessed. RNA sequencing analysis of the small intestine in the Control group and the P80 group revealed the activation of peroxisome proliferator-activated receptor α-fatty acid-binding protein1(PPARa-FABP1)axis, and further vitro experimental verification.</p><p><b><i>Results:</i></b> Emulsifier P80 intake promotes intestinal damage and accelerates intestinal aging in elderly SAMP8 mice. Furthermore, the negative effects of P80 can disrupt intestinal barrier function in aging mice and induce more senescence-associated secretory phenotype. Mechanistically, through RNA sequencing, we found that long-term intake of P80 activates the intestinal PPARa-FABP1 axis, promoting the absorption of fatty acids in the intestine, and accelerating intestinal aging by increasing oxidative stress products. Interestingly, in vitro experiments have demonstrated that P80 promotes d-galactose-induced senescence in Caco-2 cells and enhances lipid accumulation through PPAR signaling pathway, while the PPARα antagonist GW6471 could block this effect and retard senescence.</p><p><b><i>Conclusion:</i></b> Emulsifier P80 Intake increases oxidative stress and accelerates intestinal aging in SAMP8 mice through inducing intestinal fatty acid uptake and lipotoxicity.</p><p><b>PP-01-266</b></p><p><b>CT early signs (pancreatic atrophy/fat deposition) of pancreatic cancer on pre-diagnositic Images.</b></p><p><b>Noriko Ishigaki</b></p><p><i>Saiseikai Yamaguchi General Hospital, Yamaguchi-city, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Pancreatic cancer (PC) is primarily diagnosed using CT scans, but often, PC is already advanced at the time of diagnosis. It has been reported that imaging findings such as pancreatic atrophy/fat deposition (CT early signs) can be seen in early-stage PC.The aim of this study is to assess CT early signs in patients who were diagnosed with PC based on pre-diagnostic images.</p><p><b><i>Materials and Methods:</i></b> This is a retrospective observational study in our hospital. Of 132 PC patients between 2019 and 2023, we reviewed 50 patients (33 males, median age 75 [range 47-91] ) who had undergone pre-diagnostic CT scans. The primary outcome is the presence of abnormal findings of the pancreas in previous CT scans. The secondary outcomes are the changes in these findings over time.</p><p><b><i>Results:</i></b> Among 50 patients with pre-diagnostic CT (median 2 [1-13] times, conducted 3 months to 20 years before diagnosis), 39 patients (78%) had abnormal findings. These abnormal findings included pancreatic atrophy (n=25), fat deposition (n=21), duct ectasia (n=9), and cysts (n=7) (some patients had multiple abnormalities). Of the 39 patients with previous abnormal findings, 20 of 25 (80%) who had multiple CT scans showed changes over time.</p><p><b><i>Conclusion:</i></b> Pancreatic atrophy/fat deposition were observed more frequently than duct ectasia or cysts in the present study. Furthermore, these findings were often seen to progress over time. CT early signs may trigger the detection of PC, and we should pay attention to these findings as well.</p><p><b>PP-01-267</b></p><p><b>Human leukocyte an tigen DQ 2 and DQ 8 expression among renal transplanted patients chronic diarrhea</b></p><p><b>Raja Taha Yaseen Khan</b> and Abbas Ali Tasneem and Nasir Hassan Luck</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to determine the frequency of Human Leukocyte Antigen (HLA) DQ2 and DQ8 expression among renal transplant patients with DVA and negative celiac serology.</p><p><b><i>Material and Methods:</i></b> A cross-sectional study was conducted at the Sindh Institute of Urology and Transplantation (SIUT) from January to June 2021. A total of 61 renal transplant patients aged 18-60 years with DVA and negative celiac serology were included. Data collection involved detailed patient history, laboratory investigations, and HLA DQ typing. Statistical analysis was performed using SPSS version 25.0.</p><p><b><i>Results:</i></b> Among the 61 patients, 41 were males (68.3%) and 20 were females (31.7%), with a mean age of 33.8 ± 6.3 years. The predominant symptom was diarrhea (91.7%). HLA typing indicated that 56 patients (91.8%) were diagnosed with SNCD: 54 patients (90%) expressed HLA-DQ2, and 37 patients (61.7%) expressed HLA-DQ8, with 31 patients (51.7%) co-expressing both alleles. Significant associations were found between HLA-DQ2/DQ8 positivity and male gender, microcytic anemia, chronic diarrhea, and weight loss.</p><p><b><i>Conclusion:</i></b> This study demonstrates a high prevalence of HLA-DQ2 and DQ8 among renal transplant patients with DVA and negative celiac serology, suggesting a genetic predisposition to celiac disease despite negative serological markers.</p><p><b>PP-01-268</b></p><p><b>Cytomegalovirus associated colitis as a cause of lower gastrointestinal bleeding in kidney transplant recipients</b></p><p>Raja Taha Yaseen Khan, <b>Vijesh Kumar</b>, Nasir Hassan Luck and Abbas Ali Tasneem</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The aim of this study was to determine the frequency of cytomegalovirus associated colitis in Kidney transplant Recipients (KTRs) presenting with lower gastrointestinal bleeding.</p><p><b><i>Materials and Methods:</i></b> This cross-sectional study was conducted at the department of hepatogastroenterology ,Sindh Institute of Urology and Transplantation from January 2021 to December 2021.All the KTRs (6 months after the transplantation) of either gender and aged between 18 to 65 years, presenting with lower gastrointestinal (GI) bleeding were enrolled in the study. Colonic biopsies were reviewed by consultant histopathologist for the features of CMV infection.</p><p><b><i>Results:</i></b> A total of 95 renal transplant recipients of either gender or age above 18 to 65 years with the presenting with lower GI bleeding were included in the study. Most common presenting complaint was fresh bleeding per rectum which was observed in 73(76.8%). The most common finding observed on colonoscopy in KTRs with bleeding per rectum was colonic ulcers and erosions noted in 41(43.1%) and 36(37.9%) patients respectively. On histopathology, CMV Colitis was noted in 21(22.1%) patients. On comparison of different baseline variables, the presence of fresh bleeding per rectum and presence of both ulcers and erosions on colonoscopy were the factors significantly associated with CMV colitis in KTRs.</p><p><b><i>Conclusion:</i></b> CMV associated colitis was noted in 22.1% of our KTRs presenting with lower gastrointestinal bleeding. However, in our study, the levels of viremia had no significant association with the presence of CMV associated colitis.</p><p><b>PP-01-269</b></p><p><b>Differential diagnosis based on clinical symptoms and endoscopic findings in symptomatic terminal ileitis or ulcers</b></p><p><b>Woojin Kim</b>, Cheolwoong Choi and Subum Park</p><p><i>Pusan National University Yangsan Hospital, Yangsan, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The accurate identification and management of symptomatic terminal ileitis or ulcers (STIUs) remain challenging. STIUs is a common finding on ileocolonoscopy and may be related with a wide variety of diseases. The aim was the etiology and discrimination of various disease in STIUs.</p><p><b><i>Materials and Methods:</i></b> This is a retrospective study including patients with diagnosis of STIUs without colonic abnormalities on ileocolonoscopy for various symptoms in a tertiary center between January 2018 and October 2023. Data of demographics, clinical manifestations and endoscopic findings were collected.</p><p><b><i>Results:</i></b> 110 of 224 patients who had ITIUs on ileocolonoscopy have symptoms. Among 110, 71 had specific etiologies on initial testing and after 6 months follow-up. Definitive diagnosis was ascertained in Crohn’s disease (CD): 30, Behçet’s disease (BD): 20, infectious enteritis: 10, drug-induced ulcers: 5, intestinal tuberculosis (ITB): 5, lymphoma: 1, and 40 patients had nonspecific ulcers. After 1 year treatment, symptomatic and endoscopic resolution were noted in 7/30 patients with CD and 10/20 patients with BD, respectively. Of the 40 patients initially diagnosed with nonspecific ulcers with persistent symptoms, two were eventually diagnosed with CD.</p><p><b><i>Conclusions:</i></b> Disease was diagnosed in more than half of STIUs patients, with Crohn’s disease and Behçet’s disease being the most common disease in this study. Combining clinical characteristics such as oral ulcer, genital ulcer, hemoglobin and CRP with endoscopic findings can be helpful in differential diagnosis of STIUs. Therefore, accurate diagnosis and appropriate treatment through follow-up are important because of STIUs is diagnosed in half of symptomatic patients</p><p><b>PP-01-270</b></p><p><b>Causal Effects of Gut Microbiota on Viral infection: Insights from Genetic</b></p><p><b>Shuang Ma</b></p><p><i>Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Gut microbiota has been increasingly linked to the host susceptibility of virus infection, but the causal association remains unclear.</p><p><b><i>Methods:</i></b> We conducted a two-sample Mendelian randomization (MR) analysis to assess the potential causal effect of gut microbiota on viral susceptibility. Summary-level data for gut microbiota and seven viral infection outcomes were obtained from the genome-wide association study (GWAS) of the MiBioGen and FinnGen Consortium, respectively. The inverse-variance weighted (IVW) method was employed as the primary approach. Additionally, multiple sensitivity analyses were carried out to verify the robustness of the results.</p><p><b><i>Results:</i></b> After adjusting for multiple tests, we observed that the genetically predicted genera Lachnospiraceae ND3007 group (OR: 1.23, 95% CI: 1.05−1.43, q = 0.029) and Marvinbryantia (OR: 1.22, 95% CI: 1.09−1.36, q = 0.005) were causally associated with an increased risk of viral gastroenteritis, while the genus Parabacteroides (OR: 0.81, 95% CI: 0.71−0.92, q = 0.007) was associated with a decreased risk of viral gastroenteritis. Additionally, Genus Methanobrevibacter posed a protective effect against COVID-19 (OR: 0.96, 95% CI: 0.94−0.99, q = 0.029), but conversely increased the risk of herpes simplex virus (OR: 1.16, 95% CI: 1.04−1.29, q = 0.029). Sensitivity analyses confirmed the robustness of the IVW results.</p><p><b><i>Conclusion:</i></b> These findings indicate that specific gut microbiota exerts causal effects on viral infection, highlighting the potential for leveraging specific gut microbiota or their metabolites to inform strategies for preventing and managing viral infections.</p><p><b>PP-01-271</b></p><p><b>Lactobacillus johnsonii alleviates NSAID-induced enteropathy by inhibiting GSDMD-mediated pyroptosis in intestinal epithelial cells</b></p><p><b>Shuang Ma</b>, Lanping Zhu and Xin Chen</p><p><i>Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gut microbiota dysbiosis is implicated in the pathogenesis of non-steroidal anti-inflammatory drugs (NSAIDs)-related enteropathy. Lactobacillus johnsonii (L. johnsonii), a probiotic, is recognized for its ability to modulate immune microenvironments, regulate gut microbiota, and repair intestinal barriers. This study aims to explore the preventive and therapeutic effects of L. johnsonii on NSAIDs-induced enteropathy and its underlying mechanisms.</p><p><b><i>Materials and Methods:</i></b> Small intestinal injury in mice was established by indomethacin and gavaged with L. johnsonii or its supernatant. The effect of L. johnsonii in relieving small intestinal injury were assessed. The underlying mechanisms were evaluated both in vivo and in vitro.</p><p><b><i>Results:</i></b> L.johnsonii effectively alleviated indomethacin-induced small intestinal injury in mice. Mechanistically, RNA sequencing revealed downregulation of gene expression associated with the inflammasome complex and pyroptosis following L. johnsonii treatment. Additionally, L. johnsonii inhibited intestinal inflammation, restored the damaged intestinal mucosal barrier, and decreased the expression of pyroptosis-related proteins (NLRP3, Caspase1, GSDMD) in the small intestinal tissues of mice. L. johnsonii supernatant was further demonstrated to suppress pyroptosis in intestinal epithelial cells of mice in vitro, increasing cell viability and reducing LDH release as well as inflammatory cytokines IL-1β and IL-18.</p><p><b><i>Conclusion:</i></b> L.johnsonii ameliorates NSAIDs-induced enteropathy by inhibiting pyroptosis in intestinal epithelial cells via NLRP3/Caspase1/GSDMD signaling pathway. This study provides a theoretical basis for the use of probiotics in preventing and treating NSAIDs-related enteropathy, proposing new therapeutic strategies for clinical treatment.</p><p><b>PP-01-272</b></p><p><b>Chronic Diarrhea Caused by Helminthiasis: a Case Report</b></p><p><b>Maybelline Maybelline</b> and Benny Budiman</p><p><i>Mitra Medika Hospital, Pontianak, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Helminthiasis poses significant public health problems in developing countries. Consuming raw vegetables without proper washing is one of the main routes of intestinal parasite acquisition.</p><p><b><i>Case Illustration:</i></b> A 47-year-old female presented with diarrhea and abdominal pain since two weeks ago. Stools were watery, foul-smelling, with mucus, and without blood. Stool frequency was approximately 4-5 times per day. There was neither fever, weight loss, perianal itching, vomiting blood, nor dark stool. No history of allergy or asthma was reported. She had a history of consuming fresh, raw vegetables frequently. She was given ciprofloxacin 500 mg twice daily one week after the onset of diarrhea, but symptoms persisted. At admission, a complete blood count revealed leukocytosis (16.450 mm3) with monocytosis and no eosinophil elevation. No worm egg or amoeba was found on the stool examination. She was diagnosed with chronic diarrhea caused by IBD, with helminthiasis as the differential diagnosis. A colonoscopic examination demonstrated the presence of whitish parasites with a whip-like appearance on the caecum and erosion of the ascending colon. Chronic diarrhea caused by helminthiasis was diagnosed with a suspicion of Trichuris trichiura infection. She was treated with mebendazole 500 mg once daily for three days, and her symptoms improved.</p><p><b><i>Discussion:</i></b> Helminthiasis must be considered as one of the causes of chronic diarrhea. Negative worm egg found in stool examination and no eosinophilia should not rule out the possibility of helminthiasis. Antihelminthic agents, like benzimidazole-agent, are effective in treating helminthiasis.</p><p><b><i>Keywords:</i></b> Helminthiasis, colonoscopy, chronic diarrhea</p><p><b>PP-01-273</b></p><p><b>Nutrition Care after Hospital Discharge in Singapore: Evidence-Based Best-Practice Recommendations</b></p><p><b>Doris Ng</b><sup>1</sup>, Frederick Koh<sup>2</sup>, Hazel Yong<sup>3</sup>, Terence Cheong<sup>1</sup>, Min Chue Koy<sup>2</sup>, Fung Joon Foo<sup>2</sup> and Samuel Chew<sup>4</sup></p><p><sup>1</sup><i>Tan Tock Seng Hospital, Singapore, Singapore;</i> <sup>2</sup><i>SengKang General Hospital, Singapore, Singapore;</i> <sup>3</sup><i>Khoo Teck Puat Hospital, Singapore, Singapore;</i> <sup>4</sup><i>Changi General Hospital, Singapore, Singapore</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Hospitalised patients are at risk of compromised nutritional status and predisposed to further deterioration after discharge, leading to poor clinical outcomes, high healthcare costs, and poor quality of life. We aimed to provide evidence-based best-practice recommendations to address this gap in nutrition care.</p><p><b><i>Materials and Methods:</i></b> A multidisciplinary healthcare professionals (HCPs) panel from the Society of Parenteral and Enteral Nutrition (Singapore) conducted PubMed searches and summarised current literature on best practices on nutrition care after hospitalization. A national survey of HCPs from Singapore’s healthcare institutions was conducted to gain insights into existing practices and identify gaps. The panel reviewed 242 responses received and then put forth ten best-practice recommendations.</p><p><b><i>Results:</i></b> We advocate screening all patients using a validated tool with a disease activity/burden component, an easily accessible dietitian referral pathway for patients at risk of malnutrition, and an individualised nutrition care plan formulated and delivered using multidisciplinary team approaches for patients at risk or with malnutrition. A comprehensive team would include dietitians, physicians, nurses, physiotherapists, speech therapists, and medical social workers working together towards a common goal. Information on the importance of good nutrition and health and how it can be achieved should be provided to patients and their caregivers before and after hospital discharge.</p><p><b><i>Conclusion:</i></b> Nutrition screening during hospitalization and at discharge are essential to identify patients who are at risk or with malnutrition prior to discharge. With the above recommendations, we seek to improve upon the current nutrition care processes at discharge for healthcare institutions in Singapore.</p><p><b>PP-01-274</b></p><p><b>Effect of autonomic dysfunction on hemodynamic instability during per-oral endoscopic myotomy in achalasia patients</b></p><p>Min-Jae Kim, Young Hoon Youn and <b>Hyojin Park</b></p><p><i>Yousei University College Of Medicine, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Per-oral endoscopic myotomy (POEM) is a common procedure for esophageal dysphagia. CO2 insufflation during POEM helps create a submucosal tunnel but may lead to complications such as capnoperitoneum and hemodynamic instability. Patients with autonomic dysfunction might experience more severe instability during POEM, but the impact is uncertain. This study aims to investigate the effect of autonomic dysfunction on intraoperative hemodynamic instability during POEM in patients with esophageal dysphagia.</p><p><b><i>Materials and Methods:</i></b> This single-center prospective observational study began in April 2023. Participants were adult patients aged 19 years and older undergoing POEM. Exclusion criteria were pre-existing hemodynamic instability, minors, and lack of consent. All participants underwent an HRV test for autonomic dysfunction assessment the morning before POEM. The primary outcome was the wobble of systolic arterial pressure (SAP) during POEM.</p><p><b><i>Results:</i></b> By June 2024, 20 achalasia patients were enrolled. HRV tests confirmed autonomic dysfunction in 8 patients (13 males [65%]). The mean age was 40.2 years (range, 21–72). The autonomic dysfunction group showed a higher wobble of SAP (11.1 vs 12.6, mean), but the difference was not statistically significant (p=0.417). Intraoperative factors such as remifentanil infusion rate adjustments and the use of vasoactive medications (phenylephrine, ephedrine, nicardipine) were higher in the autonomic dysfunction group, with significant differences only in ephedrine use (p=0.043).</p><p><b><i>Conclusion:</i></b> Patients with achalasia and autonomic dysfunction may experience hemodynamic instability during POEM, necessitating more vigilant intraoperative monitoring.</p><p><b>PP-01-275</b></p><p><b>Comparison of Etiologies and Outcomes of Lower Gastrointestinal Bleeding Occurring in Outpatients and Inpatients</b></p><p><b>Watsamon Parkpian</b> and Supot Pongprasobchai</p><p><i>Department of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to investigate the differences between the etiologies and outcomes of inpatients vs outpatient LGIB. Methods: A retrospective cohort study of patients with LGIB who underwent colonoscopy at Siriraj Hospital from 2020 to 2021 was performed. We included outpatients and inpatient at a ratio of 3:1 and compared the demographics, presentations, etiologies, treatment, and outcomes.</p><p><b><i>Results:</i></b> There were 190 LGIB patients, 126 outpatient and 64 inpatient. The mean ages and gender were similar between both groups. Severe hematochezia was more common in inpatient than outpatient LGIB (34% vs 17%, p=0.06). The leading etiologies of outpatient LGIB were diverticular bleeding (40%), colonic cancer (7%), radiation enteritis (6%) and angioectasia (5%), while those of inpatient LGIB were acute hemorrhagic rectal ulcer syndrome (AHRUS, 17%), CMV colitis (13%), diverticular bleeding (11%), colonic cancer (9%) and ischemic colitis (5%). AHRUS was significantly more common in inpatient than outpatient LGIB (17% vs. 2%, p<0.001) while diverticular bleeding was more common in outpatient than inpatient LGIB (40% vs 11%, p<0.001). Inpatients LGIB had longer median length of stay (LOS, 33 days vs 6 days, p<0.001), higher rebleeding rate (34% vs 9%, p<0.001), and higher 30-day mortality (25% vs 3%, p<0.001). Independent factors associated with mortality were rebleeding (odds ratio [OR] 130), underlying cirrhosis (OR 28.8), initial hypotension (OR 9.6), blood transfusion (OR 1.1) and inpatient LGIB (OR 5.4).</p><p><b><i>Conclusion:</i></b> Inpatient LGIB had poorer outcomes than outpatient LGIB. The leading etiology of inpatient LGIB was AHRUS, while that of outpatient was diverticular bleeding.</p><p><b>PP-01-276</b></p><p><b>Psychological assessment of subjects with positive Fecal Immunochemical test in Phu Tho province - Vietnam</b></p><p><b>Tiến sĩ Phu Pham Quang</b><sup>1</sup>, Thuan Nghiem Duc<sup>2</sup>, Tuan Le Quoc<sup>3</sup>, Thuong Nguyen Hoai<sup>1</sup>, Nguyen NGuyen Canh<sup>2</sup>, Kim Nguyen Kim<sup>1</sup>, Lai Nguyen Van<sup>1</sup>, Linh Nguyen Thuy<sup>1</sup>, Chi Dinh Minh<sup>1</sup>, Huong Nguyen Thi Thanh<sup>1</sup> and Huong Huynh Thi Thu<sup>1</sup></p><p><sup>1</sup><i>Military Hospital 103, Hanoi, Vietnam;</i> <sup>2</sup><i>Vietnam Military Medical University, Hanoi, Vietnam;</i> <sup>3</sup><i>Thanh Ba District Medical Center, Phu Tho, Vietnam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Understanding the relationship between the patient's psychology with Fecal Immunochemical Test (FIT) positive for the decision to make a decision to screen the cause.</p><p><b><i>Materials and Methods:</i></b> Research describing cross -section over 216 patients from 40 to 80, with FIT positive after meeting the elimination standard, the time from July 2023 to May 2024. Psychiatric assessment of patient was conducted results through answering the test of anxiety, based on the scale Inventory-Form Y1 (stai-y1).</p><p><b><i>Results:</i></b> The male/female ratio is approximately 1.3/1. The average income component (5-10 million VND/month) accounts for the highest proportion (47.2%), of which the intellectual labor and manual labor account for the approximate proportion (46.1%, 53.9%). Most have no knowledge of both CRC and FIT (48.1%). Most of the endoscopic subjects find the cause of gastrointestinal pathology (81.5%). The percentage of anxiety accounts for 88%, with a low and medium level (35.6%, 39.8%). The level of anxiety according to the Stai - Y1 ladder has no association with the re -examination plan (x2 = 0.125, p = 0.974), but there is a relationship with laparoscopy to find the cause (x2 = 0.9705 and p = 0.021). In addition, there is a relationship between the endoscopic resolution and the education level (x2 = 0.877, p = 0.001) and with the groups of understanding CRC-FOBT (x2 = 0.9609, p = 0.022).</p><p><b><i>Conclusion:</i></b> There is a relationship between the patient's psychology with FIT positive, as well as the level of education, understanding of the knowledge of CRC - FIT</p><p><b>PP-01-278</b></p><p><b>A Case of Pseudo-Pseudo Meigs Syndrome (PPMS) due to SLE with Massive Ascites</b></p><p><b>Takamasa Sato</b>, Motoyuki Onodera, Yoshitaka Sakai, Takehiko Igarashi, Yuchiro Sato, Hirotaka Ito, Takuya Hara, Rina Kuamata, Masahiro Okada and Ryoto Sasaki</p><p><i>Osaki Citizen Hospital, Osaki, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> PPMS, characterized by ascites, pleural effusion, and elevated CA125, is a rare manifestation of lupus peritonitis first described in 2005. We report a case of PPMS that was difficult to diagnose.</p><p><b><i>Case Description:</i></b> A 42-year-old woman presented with chronic diarrhea, generalized edema, and abdominal distension. She had a history of necrotizing lymphadenitis and irritable bowel syndrome. She was referred to our hospital due to abdominal distension and generalized edema. Initial examination revealed hypoalbuminemia, generalized edema, and weight gain. Contrast-enhanced CT showed ascites without liver cirrhosis, portal vein thrombosis, inferior vena cava obstruction, or intestinal edema. Gastrointestinal endoscopies and protein-losing enteropathy scintigraphy were normal. Cardiac disease and nephrotic syndrome were ruled out. During the examination and follow-up, it showed worsening hypoalbuminemia, increased ascites, and new right pleural effusion. Ascites analysis revealed low SAAG, with negative cultures. Elevated serum CA125 suggested possible peritoneal carcinomatosis, but multiple cytologies were negative. Immunological tests performed due to persistent low grade fever showed elevated anti-nuclear and anti-ds-DNA antibodies and low complement levels. Re-evaluation identified Raynaud's phenomenon and chilblain-like lesions. A skin biopsy indicated lupus with a positive lupus band test, diagnosed PPMS secondary to SLE. The patient was treated with systemic and intraperitoneal glucocorticoids and immunosuppressants, resulting in a gradual reduction of ascites, improved albumin levels, and normalized CA125.</p><p><b><i>Discussion:</i></b> This case highlights the diagnostic challenges of PPMS in the absence of a prior SLE diagnosis.PPMS should be suspected in the presence of unexplained ascites effusion and the above signs.</p><p><b>PP-01-279</b></p><p><b>A Rare Case: Chilaidity Syndrome, Uncommon Abdominal Pain in Right Upper Quadran</b></p><p><b>Finly Septianto</b><sup>1</sup>, Titong Sugihartono<sup>1</sup>, Budi Widodo<sup>1</sup>, Herry Purbayu<sup>1</sup>, Tri Asih Imroati<sup>2</sup> and Annisa Zahra Mufida<sup>1,2</sup></p><p><sup>1</sup><i>RSUD dr Soetomo, Surabaya, Indonesia;</i> <sup>2</sup><i>Airlangga Teaching Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Chilaidity Syndrome is a rare condition with unique gastrointestinal symptoms due to the interposition of the colon between the liver and diaphragm caused by decreased chronic motility. This condition can lead to various complications. Understanding the etiology, diagnostic method and treatment option for Chilaidity syndrome is important.</p><p><b><i>Case Report:</i></b> A 70 years male patient presented with black starry stool and right upper quadrant abdomen pain. The pain appears when sleeping and improve when awake. Because of his complaints, patient was planning for colonoscopy. The X-rays revealed gas shadow of the intestine in the inferior of the right hemidiaphragm that confirm of Chilaidity syndrome. The colonoscopy revealed a mass on ascendent colon suspicious for adenocarcinoma well differentiated (T2N0Mx), Dukes stage A, MAC stage B1 indicated for surgery. The laboratories revealed slight anemia while the other are normal. Ultrasound abdomen revealed Benign Prostate Hyperplasia and minimal ascites in the M’c Burney while CT-Scan abdomen with contrast perform with no mass shadow results.</p><p><b><i>Discussion:</i></b> The Placement of the colon or small intestine between the liver and right diaphragm in the absence of symptoms is known as the Chilaidity sign. Abdominal pain is one of the symptoms associated with the Chilaidity sign, a disorder known as Chilaidity syndrome. Conservative is the best treatment. It is important to consider Chilaidity syndrome presents with predisposing factor such as GIT cancers. Imaging studies, such as X-rays or CT-Scan, play a crucial role for confirm the diagnosis.</p><p><b>PP-01-280</b></p><p><b>Paraneoplastic pseudoachalasia secondary to peritoneal sarcomatoid mesothelioma</b></p><p><b>Charlene Tan</b><sup>1</sup>, Paolo Francisco Carlos<sup>2</sup>, Yi Yuan Tan<sup>1,3</sup>, Kim Wei Lim<sup>1,3</sup>, Asokkumar Ravishankar<sup>1,3</sup> and R Rajesh<sup>1,3</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Hepatology, Singapore General Hospital;</i> <sup>2</sup><i>Institute of Digestive and Liver Diseases, St Luke’s Medical Centre- Global City, Philippines;</i> <sup>3</sup><i>DUKE-NUS Graduate Medical School, Singapore</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Paraneoplastic pseudoachalasia is a rare finding that is most commonly associated with gastrointestinal malignancies. We describe an unusual case of paraneoplastic pseudoachalasia associated with peritoneal sarcomatoid mesothelioma.</p><p><b><i>Case Description:</i></b> We present a case of a 56 year old male who presented to our emergency department with lower abdominal distension, dysphagia and early satiety. Computed topography of the thorax, abdomen and pelvis showed a distended oesophagus with abrupt change in caliber at the gastroesophageal junction (GEJ), a dilated appendix, as well as peritoneal thickening and caking suspicious for peritoneal disease. Endoscopic evaluation showed narrowing of the GEJ with visible puckering but no signs of underlying mass, stricture or oesophagitis. Multiple biopsies were taken during endoscopic evaluation but histologic examination of the samples was unremarkable. Barium swallow showed findings consistent with achalasia. The stenosis was treated by dilatation and ultrasound-guided peritoneal biopsy was performed, with histology consistent with sarcomatoid mesothelioma. The patient was subsequently diagnosed with metastatic peritoneal sarcomatoid mesothelioma with paraneoplastic pseudochalasia.</p><p><b><i>Discussion:</i></b> Pseudoachalasia can present similarly to achalasia and a high index of suspicion is needed especially in patients of advanced age, with a short onset of symptoms and significant loss of weight. Further investigations should be pursued to exclude an underlying malignancy. When there is no direct infiltration or compression of the GEJ, paraneoplastic pseudo-achalasia from a distant source should be considered.</p><p><b>PP-01-281</b></p><p><b>Differences in Neutrophil-Lymphocyte Ratio in Dyspepsia Syndrome Patients with and without Peptic Ulcer</b></p><p><b>Pebria Suryani Valentin</b><sup>1</sup>, Bradley Waleleng<sup>2</sup>, Luciana Rotty<sup>2</sup>, Fandy Gosal<sup>2</sup>, Jeanne Winarta<sup>2</sup> and Andrew Waleleng<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University, Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University, Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Peptic ulcer causes long-term inflammation resulting in changes in the stomach and duodenum movement and sensitivity, becoming the most common cause of dyspepsia. However, there have not been many studies on dyspeptic patients with peptic ulcers. A recent study by Coşgun and Aras in 2023 mentioned that patients with peptic ulcers have higher NLR values compared to patients without peptic ulcers. Neutrophil-lymphocyte ratio (NLR) is a cost-effective and efficient biomarker associated with several inflammatory disorders. This study aims to analyze the difference in NLR values in dyspepsia patients with and without peptic ulcers at Prof. R.D. Kandou Hospital Manado in the period January-April 2024.</p><p><b><i>Materials and Methods:</i></b> A cross-sectional observational study was conducted on patients diagnosed with dyspepsia and underwent endoscopy at RSUP Prof. R. D. Kandou Manado from January to April 2024. Data were not normally distributed so associations were analyzed using Mann Whitney U test. The p-value of <0.05 was considered significant.</p><p><b><i>Results:</i></b> A total of 53 research subjects were included in this study, most of whom were male (54.7%) with average age of 56.39 ± 12.99 years. Endoscopy showed that 15 patients (28.3%) had peptic ulcers and 38 patients (71.7%) without peptic ulcers. The NLR value was significantly higher in patients with peptic ulcers (5.35 ± 7.67) compared to patients without peptic ulcers (2.82 ± 3.11, p value=0.037).</p><p><b><i>Conclusion:</i></b> Patients with peptic ulcers have higher NLR values compared to patients without peptic ulcers.</p><p><b>PP-01-282</b></p><p><b>Investigation of the Factors Necessary to Achieve Diversity and Inclusion among Japanese GI Physicians</b></p><p><b>Takanori Yamada</b></p><p><i>Hamamatsu University School Of Medicine, Hamamatsu, Japan</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Diversity has revealed that even though a variety of skills and ideas exist, a psychologically safety is necessary to make the most of them (inclusion). We attempted to determine what factors are necessary for the practice of gastroenterology to be inclusive of all individuals and to achieve both productivity and personal well-being.</p><p><b><i>Materials and Methods:</i></b> Questionnaires were administered on physician profile, job satisfaction, personal satisfaction, Utrecht Work Engagement Scale, and AC Edmondson psychological safety score.</p><p><b><i>Results:</i></b> Forty-one GI physicians from five institutions responded to the survey. Six residents, 16 consultants, 12 instructors, and 7 others, 43.5±39.2 hours/month of overtime, 4.9±1.8 days/month of non-work days, 13.4±10.8 hours/week of housework/child care, 33.0±10.0/54 points on the engagement scale, 37.5±7.0/49 points on the psychological safety score The results were. Team productivity was correlated with job satisfaction (ρ=0.481) and holiday satisfaction (ρ=0.425), and own productivity was correlated with evaluation of skills (ρ=0.441) among psychological safety items, but not with the total psychological safety score (ρ=0.134). Evaluation of skills correlated more strongly with total engagement (ρ=0.461), of which vitality (ρ=0.496) and enthusiasm (ρ=0.537) that the job provided. Furthermore, team productivity, job satisfaction showed stronger correlations with holiday satisfaction (ρ=0.687), personal satisfaction (ρ=0.672), number of days away from work (ρ=0.495), total psychological safety (ρ=0.363), and among these, ease of proposing difficulties and difficult problems (ρ=0.547).</p><p><b><i>Conclusion:</i></b> It was suggested that evaluation of skills, ease of proposals, and enrichment of holidays and personal life may contribute to improving GI physicians' job satisfaction and performance</p><p><b>PP-01-283</b></p><p><b>Gastirc microbiota of malignant transformation of gastric proliferative polyps</b></p><p><b>Dongxue Zhang</b>, Jing Ning, Xin Liu, Zhanyue Niu, Jing Zhang and Shigang Ding</p><p><i>北京大学第三医院, Beijing, 中国</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Object:</i></b> To explore differences of microflora between gastric gastric proliferative and high-grade intraepithelial neoplasia of gastric hyperplastic polyps, and explore the further pathogenesis.</p><p><b><i>Method:</i></b> Patients with gastric hyperplastic polyps and gastric hyperplastic polyps with high-grade intraepithelial neoplasia (high-grade dysplasia and carcinogenesis) diagnosed pathologically were selected and matched according to age and sex, with 10 patients in each group. Paraffin embedded specimens of their tissues were collected. 16SrRNA high-throughput sequencing and bioinformatics were used to analyze the diversity and species composition of gastric hyperplastic polyp in two groups of patients.</p><p><b><i>Results:</i></b> There was no significant difference in the α-diversity of the bacterial microbiota between the two groups, but there was a difference in β-diversity. The bacterial abundance measurement showed that Shewanella(ASV) was higher in patients with high-grade intraepithelial neoplasia of gastric hyperplastic polyps than in patients with simple gastric hyperplastic polyps, while ASV of Sphingomonadaceae and Flavisolibacte was decreased (both P<0.05).</p><p>LEfSe analysis revealed that Acinetobacter, Shewanella and Ramlibacter were enriched in patients with high-grade intraepithelial neoplasia of gastric hyperplastic polyps, while Rubrobacte, Sphingomonadaceae-unclassified and Lysinibacillus were enriched in the patients with simple gastric hyperplastic polyps.</p><p><b><i>Conclusion:</i></b> Patients with gastric hyperplastic polyps with high-grade intraepithelial neoplasia and patients with gastric hyperplastic polyps alone have different microflora characteristics, the specific mechanism of which is still unclear and needs to be further revealed.</p><p><b>PP-01-284</b></p><p><b>Diagnostic Value of Spot Urine Na/K Ratio Compared to 24-hour Urine Sodium in cirrhotic patients</b></p><p><b>Sukanta Chandra Das</b><sup>1</sup> and Naymul Hasan<sup>2</sup></p><p><sup>1</sup><i>Kurmitola General Hospital, Dhaka, Bangladesh;</i> <sup>2</sup><i>Shaheed ziaur Rahma medical college, Bogra, Bangladesh</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ascites is a major complication of liver cirrhosis that carries a poor prognosis. Monitoring of diuretic response can be achieved by measurement of 24 hour urinary sodium. The “spot” urine sodium/potassium (UNa/K) ratio can be a convenient tool to identify 24 hour urinary sodium excretion.</p><p><b><i>Materials and Methods:</i></b> This was a cross-sectional study to evaluate natriuresis in patients with decompensated liver cirrhosis with ascites admitted in Banghabandhu sheikh mujib medical university. 100 patients were included in this study.The Na/Ku ratio was calculated based on the values of sodium and potassium in “spot” urine sample which was obtained before or after completion of 24-hr urine collection. Instructions was given to assure completeness of urine collection.The correlation between the 24hr-UNa and spot UNa/K ratio was evaluated by the Pearson’s correlation coefficient. Diagnostic accuracy of the spot UNa/K ratio was analysed by estimating the area under the receiver operating characteristics curve (AUROC) and by calculating accuracy, sensitivity, specificity, positive and negative predictive value.</p><p><b><i>Results:</i></b> Spot urine Na/K ratio has adequate sensitivity and accuracy for assessment of natriuresis compared with 24 hr urinary sodium in cirrhotic patients with ascites at cut off value 1.</p><p><b><i>Conclusion:</i></b> So, spot UNa/K ratio is an accurate, cost-effective and convenient method for replacing 24-hr UNa. But large multicentred studies are needed to recommend this test as a routine.</p><p><b>PP-01-285</b></p><p><b>Non-invasively Differentiate Non-alcoholic Steatohepatitis by Visualizing Hepatic Integrin αvβ3 Expression with Molecular Imaging</b></p><p><b>Ling Wu</b>, Chenyi Rao, Xiaoquan Huang and Shiyao Chen and Feng Li</p><p><i>Zhongshan Hospital, Fudan University, Shanghai, China</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Currently, Early identification of patients with metabolism associated steatohepatitis (MASH) is essential to prevent progression and outcome of patients. This study aimed to develop an integrin αvβ3-targeted molecular imaging modality to grade and stage MASH non-invasively.</p><p><b><i>Materials and Methods:</i></b> Integrin αvβ3 expression was determined in LO2 hepatocytes cultured with palmitate acid and oleic acid (FFA). Hepatic integrin αvβ3 expression was assessed in rabbits an d rats fed with a high-fat diet (HFD) or high-fat high-carbohydrate diet (HFCD). An imaging construct containing cyclic arginine-glycine-aspartic acid (cRGD) synthesized and labeled with gadolinium (Gd) was used as a contrast agent, and magnetic resonance imaging (MRI) was performed in mice fed the HFCD.</p><p><b><i>Results:</i></b> Integrin αvβ3 was markedly expressed by FFA-cultured hepatocytes but not by normal hepatocytes. In both HFD-fed rabbits and rats, hepatic integrin αvβ3 expression was significantly increased when simple fatty liver (FL) progressed to steatohepatitis. The distribution of integrin αvβ3 in the livers with MASH overlapped with albumin-stained hepatocytes. Compared to mice with simple FL, the relative liver MRI-T1 signal value at 60 minutes post-injection of Gd-labeled cRGD was significantly increased in mice with histological steatohepatitis (P < 0.05), and showed a positive correlation with the MAFLD activity score (r = 0.945, P < 0.01).</p><p><b><i>Conclusion:</i></b> Hepatic integrin αvβ3 expression was significantly increased during the development progression of MASH, and hepatocytes were primary cells that highly upregulated integrin αvβ3 in MASH livers. Our Gd-labeled cRGD MRI tracer is a promising agent to quantitatively image MASH activity over the whole liver.</p><p><b>PP-01-286</b></p><p><b>Alcohol-driven gut dysbiosis triggers linoleic acid accumulation and inflammation in rat-model of Alcohol-associated liver disease</b></p><p><b>Manisha Yadav</b>, Neha Sharma, Sadam Bhat, Nupur Sharma, Gaurav Tripathi, Vasundhra Bindal, Babu Mathew, Sushmita Pandey, Shiv Kumar Sarin and Jaswinder Singh Maras</p><p><i>Institute of Liver and Biliary Sciences, New Delhi, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To study systemic metabolomic changes induced due to alcohol associated microbiome and healthy microbiome post FMT in Alcohol associated liver disease.</p><p><b><i>Material and Methods:</i></b> We utilized humanized rats to assess how altered microbiota and microbiome affects metabolism. Criss-cross FMT involved transplanting healthy-donor microbiota into ALD-rats and ALD-patient microbiota into healthy-rats was performed. Metabolomics and metaproteomic analysis were performed in liver, plasma, intestine, and stool.</p><p><b><i>Results:</i></b> Chronic ethanol consumption in Long-Evans rats disrupted metabolic pathways in liver, intestine, stool, and plasma, including butanoate, bile, arachidonic acid, linoleic acid, and microbial pathways. Metaproteome analysis revealed decreased levels of Lactobacillus species (L. delbrueckii and L. plantarum), leading to increased accumulation of linoleic acid in liver and intestine. Elevated linoleic acid levels correlated with inflammation and oxidative stress, accompanied by upregulation of cyp1a1 mRNA expression associated with linoleic and arachidonic acid metabolism.</p><p>In rats with alcoholic liver disease (ALD), fecal microbiota transplantation (FMT) from healthy donors restored Lactobacillus species, reduced inflammation, ROS production, and normalized cyp1a1 expression and linoleic acid metabolism. Conversely, transplantation of alcohol-associated microbiota from ALD patients to healthy rats significantly increased cyp1a1 expression, inflammation, ROS production, and linoleic acid metabolism.</p><p><b><i>Conclusion:</i></b> Our findings suggest that chronic alcohol consumption reduces beneficial Lactobacillus species in the intestine, leading to linoleic acid accumulation, inflammation, and ROS production. FMT with microbiota capable of metabolizing linoleic acid into anti-inflammatory products restores intestinal health. Targeting linoleic acid metabolism and using Lactobacillus strains as probiotics could potentially offer therapeutic benefits in alcoholic liver disease.</p><p><b>PP-01-287</b></p><p><b>Not The Usual: A Large Well- Differentiated, Non- AFP Producing HCC with Atypical Imaging Features</b></p><p><b>Christine Velasquez</b> and Julieta G. Cervantes</p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hepatocellular carcinoma (HCC), the 6th most common cancer presents with varying characteristics that lead to atypical imaging findings and diagnostic challenges. HCC progresses from pre-neoplastic lesions to early HCC, which is well-differentiated. As it grows, it de-differentiates. Well-differentiated HCC over 3 cm is rare and has distinct characteristics compared to typical HCC.</p><p><b><i>Case Description:</i></b> A 66-year-old male presented with a three-month history of progressive right upper quadrant pain, easy fatigability, and weight loss. Initial workups revealed elevated liver enzymes, prolonged INR, previous hepatitis B infection, and normal tumor markers. Abdominal ultrasound showed cirrhosis and multiple liver masses with malignant characteristics. PET scan was negative for metastasis. Percutaneous liver biopsy was equivocal, showing atypical malignant cells (CK7+, CK20-). Further diagnostic imaging included elastography (F4) for fibrosis staging and four-phase dynamic CT to describe lesions. LI-RADS system showed arterially enhancing lesions (LR3,LR4,LRM) with no washout. The impression was atypical HCC versus combined HCC-cholangiocarcinoma. MRI of the Liver also showed early enhancing foci without demonstrable washout, confirming the CT findings. Results were indeterminate, hence laparoscopic-guided biopsy was done showing d well-differentiated HCC. The patient was classified as BCLC C (lesion characteristics, Child-Pugh 7B, ECOG1) and started on systemic therapy with Lenvatinib which was tolerated.</p><p><b><i>Discussion:</i></b> Due to the complexity of hepatocarcinogenesis, HCC can have atypical imaging findings, making diagnosis difficult. The classical arterial phase enhancement and portal/delayed phase washout are not always seen. Establishing a definitive diagnosis is crucial for management decisions, and a biopsy is performed when imaging modalities are inconclusive.</p><p><b>PP-01-288</b></p><p><b>Standard-Dose Ursodeoxycholic Acid Improves Liver Function in Chronic Liver Disease: A Randomized, Double-Blind, Placebo-Controlled Trial</b></p><p>Young Chang and Yong Kyun Cho and Young Seok Kim and Sung-Eun Kim and Gab Jin Cheon and Ji Hoon Kim and Hyun Yang and Won Kim and Sang Bong Ahn and Eileen L Yoon and Jae-Youn Cheong and Jin-Woo Lee and Moon Young Kim and Hyung Joon Kim and Sae Hwan Lee and Eun Young Cho and Na Ryung Choi and Hye Won Lee and Kang Mo Kim and Won Hyeok Choe and Jung Min Yu and Sun Young Lee and Hye Jung Lee and Su Young Kim and <b>Jae Young Jang</b></p><p><i>Soonchunhyang University, Seoul, South Korea</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study assessed the efficacy and safety of ursodeoxycholic acid (UDCA), administered at 100 mg three times a day, compared to placebo in patients with chronic liver disease.</p><p><b><i>Methods:</i></b> We conducted a multicenter, double-blind, placebo-controlled, randomized phase IV clinical trial in academic hospitals in South Korea. Patients with chronic liver disease and abnormal serum ALT levels in at least two consecutive results prior to screening, persisting for at least six months, were randomly assigned to the treatment or placebo group. Patients received UDCA 100 mg or placebo three times a day for 8 weeks. The primary endpoint was the mean relative change in ALT concentrations from baseline. Post-treatment assessments included changes in fibrosis measured using FibroTest and drug-related adverse events.</p><p><b><i>Results:</i></b> Between February 2023 and March 2024, 263 patients were analyzed: 132 in the UDCA group and 131 in the placebo group. At week 8, UDCA-treated patients showed a significantly greater reduction in serum ALT from baseline compared to the placebo group (-14.70 U/L vs. -5.51 U/L; p=0.0104). The percent change was also greater in the UDCA group (-17.43% vs. -5.82%; p=0.0112). ALT normalization was higher in the UDCA group (26.52% vs. 13.08%; p=0.0050). Fibrosis reduction was greater in the UDCA group (-0.03 vs. -0.00; p=0.0157). Adverse event frequencies were similar, with no serious adverse events in the UDCA group.</p><p><b><i>Conclusions:</i></b> UDCA 100 mg three times a day for 8 weeks demonstrated superior therapeutic efficacy and a favorable safety profile compared to placebo in patients with chronic liver disease.</p><p><b>PP-01-289</b></p><p><b>A 19-Year Old Female Patient with Congenital Absence of the Portal Vein: A Rare Case</b></p><p><b>Cecilia Oktaria Permatadewi</b><sup>1</sup>, Achmad Fathi Fuadi<sup>2</sup>, Erick Prabowo<sup>2</sup>, Antonius Gunawan Santoso<sup>3</sup>, Didik Indiarso<sup>1</sup>, Agung Prasetyo<sup>1</sup>, Hesti Triwahyu Hutami<sup>1</sup>, Hirlan Hirlan<sup>1</sup> and Hery Djagat Purnomo<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroentero- Hepatology, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia;</i> <sup>2</sup><i>Department of Digestive Surgery, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia;</i> <sup>3</sup><i>Department of Radiology, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Congenital absence of the portal vein (CAPV) is a rare congenital anomaly involving the absence of the portal vein. Understanding CAPV's clinical presentation and diagnostic approach is crucial for appropriate management.</p><p><b><i>Case Report:</i></b> We report a case of a 19-year-old girl who presented with recurrent haematemesis and melena since she was 5-year-old. Physical examination revealed enlarged spleen. She was severely anemic, with a hemoglobin level of 4.6 g/dL. Initial laboratory tests showed a mild liver dysfunction. CT-Scan Abdomen revealed the absence of the portal vein with extensive collateral circulation, splenomegaly with infarction in the superior-lateral region, dilation and tortuosity of the splenic vein, superior mesenteric vein, and the post-splenic system veins (gastric vein, esophageal vein, and inferior mesenteric vein) which connected to the left renal vein.</p><p>Angiography found that the superior mesenteric vein and portosystemic vein were joined, forming collateral to the renal vein and subdiaphragmatic IVC, confirmed the diagnosis of CAPV type Ib. Echocardiogram before surgery revealed good ventricular function. No other abnormal congenital or genetic condition was detected. The patient underwent splenectomy and partial devascularisation surgery. Post surgery she was conscious and no evidence of GI bleeding.</p><p><b><i>Discussion:</i></b> CAPV is a rare condition with clinical presentations that can range from no symptoms to systemic manifestations such as hepatopulmonary syndrome, encephalopathy, or hepatoma. Previous reports indicated the risk of HCC in CAVP patient. This case represent our experience in these rare and complex patient. Long term monitoring is necessary to identify any potential complications.</p><p><b>PP-01-290</b></p><p><b>Financial Toxicity of Colorectal Carcinoma in Sri Lanka: Preliminary Results</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Mithushan Jesuthasan<sup>1</sup>, Githma Wimalasena<sup>1</sup>, Duminda Subasinghe<sup>1,2</sup>, Dakshith Wickramasinghe<sup>1,2</sup> and Sanjeewa Senevirathne<sup>1,2</sup></p><p><sup>1</sup><i>Department Of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital, Sri Lanka, Colombo</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Financial toxicity in cancer refers to the excessive financial strain on patients, families, and society due to cancer-related expenses. This study assesses the financial toxicity in a cohort of Sri Lankan patients with colorectal cancer.</p><p><b><i>Materials and Methods:</i></b> Data from patients with colorectal carcinoma of any stage, diagnosed 6- 24 months prior, and receiving treatment from the National Cancer Institute were collected using an interviewer-administered questionnaire.</p><p><b><i>Results:</i></b> Eighty-one patients were included. Financial toxicity was highly prevalent with 82.7%; (n=67) reporting a financial toxicity of 4 or 5 on a scale of 1 to 5. Transport (72, 88.9%), external investigations (55, 67.9%), supportive medications (53, 65.4%), and active cancer management (48, 59.3%) were the major factors contributing to financial burden. The main impacts of financial toxicity included compromised spending on food (40, 49.4%), children's education (23, 28.4%), and recreation (26, 32.1%). Financial toxicity resulted in 56 (69.1%) exhausting life savings, 35 (43.2%) losing property and 28 (34.6%) resorting to loans. Financial aid was received by 45 (55.6%), primarily from their relatives (36, 44.4%), with minimal support from the government (n=6, 7.4%) or the hospital (n=2, 2.5%).</p><p><b><i>Conclusion:</i></b> Despite free healthcare in Sri Lanka, patients with colorectal carcinoma seeking treatment from the public health sector were found to be facing significant financial toxicity, often compromising basic day-to-day needs. Many had exhausted their life savings, lost property or had to resort to loans impacting the future economic stability of the whole household.</p><p><b>PP-01-291</b></p><p><b>Clinical characteristics and outcomes in hilar cholangiocarcinoma: A 6-year experience</b></p><p><b>Dulanja Senanayake</b><sup>1</sup>, Mithushan Jesuthasan<sup>1</sup>, Duminda Subasinghe<sup>2,3</sup>, Nilesh Fernandopulle<sup>1,3</sup>, Vihara Dissanayake<sup>4</sup> and Sivasooriya Siwaganesh<sup>2,3</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, National Hospital Sri Lanka, Sri Lanka;</i> <sup>4</sup><i>Department of Anesthesiology and Critical Care, University of Colombo</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Hilar cholangiocarcinomas (HC) are associated with poor outcomes. This study outlines the demography and outcomes of patients with HC from a single HPB surgical unit in Sri Lanka.</p><p><b><i>Materials and Methods:</i></b> A retrospective analysis of the unit HC database from 2019 to 2024 was done.</p><p><b><i>Results:</i></b> Forty-eight patients were included, with a mean age of 63.5 years; 29(60.4%) were male. Majority were ASA II (n=19,39.6%). The most common symptom was obstructive jaundice (n=37,77.1%), followed by anorexia and weight loss (n=30,62.5%). The mean duration of symptoms on presentation was 3 months. Most tumours were Bismuth type 4 (n=12,25.0%) and type 1(n=3, 6.3%), type 2 (n=9, 18.8%), type 3A (n=5, 10.4%), type 3B(n=6,12.5%). Staging laparoscopy was performed in 22 (45.8%) patients, with 11 revealing metastatic disease. 39 (77.1%) patients were inoperable, and only 9(18.8%) lesions were resectable. However, surgery was performed in only 6 (12.5%). There were two patients with poor performance status and one with cirrhosis which precluded curative resection. The majority (n=42,87.5%) received palliative care. Metastatic disease was found in 23(47.9%). Surgical procedures included extended right hepatectomy+extrahepatic biliary resection(EHBR) and hepaticojejunostomy (n=4) and left hepatectomy with EHBR+hepaticojejunostomy(n=2). Other interventions included ERCP+palliative biliary stenting (n=17,35.4%), external biliary drainage (n=15,31.3%), PTC (n=6,12.5%),EUS-BD (Hep-Gas) (n=1). Post-operative complications included pneumonia(n=3) and transient liver dysfunction(n=2). There was one postoperative mortality due to post-hepatectomy liver failure.</p><p><b><i>Conclusion:</i></b> Locally advanced or metastatic disease and poor patient fitness have led to poor outcomes for hilar cholangiocarcinoma. Early referral to specialized surgical units should be emphasized.</p><p><b>PP-01-292</b></p><p><b>Subtotal cholecystectomy: a decade’s experience of a tertiary Hepatobilliary Surgical Unit</b></p><p>Githma Wimalasena<sup>1</sup>, Dulanja Senanayake<sup>1</sup>, S Sivaganesh<sup>2,3</sup> and <b>Duminda Subasinghe</b><sup>2,3</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Laparoscopic cholecystectomy (LC) is the standard of care for symptomatic gallstone disease. Where visualisation of the cysto-hepatic triangle is compromised a subtotal cholecystectomy (SC) is a safe alternative to minimize bile duct injuries. This may be done laparoscopically or as an open procedure depending on the experience of the surgeon. This study describes the experience of a HPB surgical unit in SC.</p><p><b><i>Materials and Methods:</i></b> A retrospective analysis of the unit LC database containing patient demography and operative notes from year 2015 to 2024 was done.</p><p><b><i>Results:</i></b> Of the 284 LCs done during this period, 23 (8.1%) were SCs. The mean age of patients who underwent SC was 53.13 (SD – 16.75) years with Male : Female ratio 1 : 0.92. Of the SCs, 9 (39.13%) were performed laparoscopically and 14 were done as open procedures. Failure to demonstrate the cysto-hepatic triangle due to dense adhesions (n=20, 86.96%), an impacted stone in Hartmann’s pouch (n=2,8.69%) and presence of a cholecysto-duodenal fistula (n=1,4.35%) were indications for SC. The mean post-operative hospital stay was shorter for laparoscopic SC (1.44 days, SD-0.527) than open SC (4.38 days, SD-2.567) and was found to be statistically significant (p = 0.001). There was one postoperative mortality due to biliary sepsis. 12 (52.2%) patients who underwent SCs followed up over a mean period of 20.6 months were well without any recurrences.</p><p><b><i>Conclusion:</i></b> SCs are a safe option for difficult gallbladders. Laparoscopic SCs have a significantly shorter postoperative hospital stay than open SCs.</p><p><b>PP-01-293</b></p><p><b>Ileocolic Intussusception in the Elderly: A Case Report</b></p><p><b>Fita Fitrianti</b><sup>1</sup>, Arlyando Saragih<sup>1</sup> and Febiansyah Ibrahim<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Hermina Jatinegara, Jakarta Timur, Indonesia;</i> <sup>2</sup><i>Digestive Division, Department of Surgery, Hermina Jatinegara Hospital, Jakarta Timur, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Intussusception is a condition in which a segment of the gastrointestinal tract invaginates into the lumen of another segment and recognised as a cause of bowel obstruction. Adult intussusception is less common than juvenile intussusception in terms of cause, appearance, and treatment.</p><p><b><i>Case Description:</i></b> We report the case of a 71-year-old female patient who presented to the Emergency Department (ED) with upper abdominal pain, nausea, vomiting and weakness. The patient had ongoing mild intermittent abdominal pain for months. There were features of peritonism on clinical examination and this was associated with raised inflammatory markers. A Computed Tomography (CT) scan showed Ileocolic intussusception. The patient underwent emergency surgical resection. Meckel's diverticulum was found as a pathologic lead point in the resected specimen, with no evidence of malignancy.</p><p><b><i>Discussion:</i></b> We presented a case of in an adult which is not commonly seen. Clinical history of chronic intermittent abdominal pain and CT abdomen are helpful in establishing the diagnosis. Despite that conservative approach is described in the literature, surgery continues to be the only option in patients who are unstable and show signs of peritonitis.</p><p><b>PP-01-294</b></p><p><b>The Masquerade of Hepatosplenic T-cell Lymphoma</b></p><p><b>Syuhada Dan Adnan</b></p><p><i>Hospital Sultanah Nur Zahirah, Kuala Terengganu, Kuala Terengganu, Malaysia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p>Hepatosplenic T-cell lymphoma (HSTCL) is an extremely rare and aggressive form of lymphoma. Unlike most common forms of lymphoma, swollen lymph nodes are uncommon in HCTL. Patients usually present with non specific constitutional symptoms and spleen and liver enlargement, with variable degree of cytopenia. The rarity of this disease, coupled with lack of lymph node involvement that usually seen in lymphomas, causes significantly difficulty in diagnosis and inevitably delays the initiation of treatment. The author reports the case of a young lady who admitted with jaundice and cytopenia. The diagnosis was challenging, initially mimicking autoimmune disease and it required an extensive investigation that finally revealed the ultimate diagnosis. This case highlights the difficulty in diagnosing HSTCL and the importance of considering diagnosis of hepatosplenomegaly in patients who present with constitutional symptoms and no significant lymphadenopathy.</p><p><b>PP-01-296</b></p><p><b>Relationship between simultaneous expression of ALDH and KRAS with endoscopic, histological characteristics of gastric cancer</b></p><p><b>Hoang Dong Duc</b><sup>1</sup> and An Le Viet<sup>2</sup></p><p><sup>1</sup><i>Thai Nguyen University Of Medicine And Pharmacy, Thai Nguyen, Viet Nam;</i> <sup>2</sup><i>Tien Du District Medical Center, Bac Ninh, Viet Nam</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To analyze the relationship between the simultaneous expression of ALDH and KRAS with endoscopic, histological characteristics in gastric cancer (GC).</p><p><b><i>Materials and Methods:</i></b> Cross-sectional study on 103 patients with a confirmed diagnosis of gastric cancer and surgical resection of the tumor at K Hospital, Ha Noi, Viet Nam. Analysis of the relationship between the simultaneous expression of ALDH, KRAS, and endoscopic, histological features.</p><p><b><i>Results:</i></b> The rate of GC in men and women was highest in the age group 60-69, accounting for 34.4% and 35.7%, respectively. Patients with GC in the antrum had the highest rate of simultaneous expression of both markers (56.0%), p > 0.05. Patients with ulcerative tumors expressing both markers had the highest rate (68.0%), p > 0.05. Patients with tubular adenoma had the highest rate of co-expression of both markers (66.0%), p > 0.05. Patients with low and moderately differentiated GC had the highest rate of simultaneous expression of both markers at 34.0%, with a difference in the simultaneous expression of both markers according to the degree of differentiation, p < 0, 05.</p><p><b><i>Conclusion:</i></b> Simultaneous expression of ALDH and KRAS is associated with low and moderate differentiation according to histopathology. Simultaneous expression of ALDH and KRAS is not associated with endoscopic characteristics.</p><p><b>PP-01-301</b></p><p><b>Serum Interleukin 17 Level in Ulcerative Colitis and Crohn’s Disease: Inflammatory Bowel Disease Patients in Indonesia</b></p><p><b>Muhammad Faisal Prananda</b>, Murdani Abdullah, Sukamto Koesnoe, Robert Sinto, Dadang Makmun and Ayu Suciah Khaerani</p><p><i>Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The incidence of IBD is increasing worldwide, with different characteristics in each country. IL-17 is a strong pro-inflammatory cytokine that plays a role in IBD. No studies have assessed serum interleukin 17 (IL-17) levels in IBD patients in Indonesia, which can reveal its role in the pathogenesis of IBD as well as proposed therapeutic modalities for IBD.</p><p><b><i>Aim:</i></b> To determine the difference in serum IL-17 levels in patients with IBD.</p><p><b><i>Method:</i></b> We conducted a cross-sectional study at Cipto Mangunkusumo National General Hospital from April 2022 to April 2023. The inclusion criteria were adult patients diagnosed with IBD and a healthy population who did not have any gastrointestinal complaints or a history of autoimmune diseases and malignancies. Data collection was carried out sequentially. Serum IL-17 levels were assessed using ELISA. Mann-Whitney tests and linear regression were carried out using the SPSS application.</p><p><b><i>Result:</i></b> The total number of subjects in this study was 125, consisting of 93 IBD patients and 32 healthy subjects. Serum IL-17 levels were 4.13 (3.19-5.14), 4.30 (3.59-5.14), and 3.40 (2.97-4.01) pg/mL for the ulcerative colitis (UC), Crohn’s disease (CD), and healthy subjects (p=0.004). There was no difference of serum IL-17 levels between UC and CD. Multivariate analysis showed that disease activity (p=0.010) is associated with serum IL-17 levels in UC.</p><p><b><i>Conclusion:</i></b> There was a significant difference in the median value of serum IL-17 levels between IBD and healthy population, but there was no differences between UC and CD.</p><p><b>PP-01-318</b></p><p><b>Pharmacological inhibition of HMGB1 ameliorates sepsis induced hepatic micro-vascular dysfunction and portal hypertension during cirrhosis</b></p><p><b>Vaibhav Tiwari</b>, Rajni Yadav, Aishwarya Bhatnagar, S Himanshi, Savneet Kaur and Dinesh Mani Tripathi</p><p><i>Institute of Liver and Biliary Sciences, New Delhi, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the role of HMGB1 in sepsis induced hepatic microvascular-dysfunction and portal-hypertension in cirrhosis.</p><p><b><i>Materials and Methods:</i></b> Experimentally, polymicrobial sepsis was induced by caecum ligation and puncture in non cirrhotic animals and by intraperitonial administration of LPS 1mg/kg in CCl4-cirrhotic animals.Controls(Ct1), Ct+CLP(Ct2), Ct+CLP+Glycyrrhizin(Ct3) were compared with CCl4 cirrhotic rats(Gr1), CCl4+LPS(Gr2), CCl4+LPS+Glycyrrhizin(Gr3). HMGB1 pharmacological inhibition was achieved by glycyrrhizin(GLZ). Hepatic hemodynamic was monitored followed by in-situ-exvivo Hepatic microvascular functionality analysis. Cellular, Molecular, Biochemical and histological analysis was performed in hepatic tissues.</p><p><b><i>Results:</i></b> Studies shows a noticeable increased PP( 9.2±1; + 27.7% (CT2), vs 6.1±1 in CT1) a marked reduction in CT3 (8.1±.5; -15.2%) vs CT2(p=0.01) was observed.CT2 animals also had increased SMABF (p=0.001) and PBF (p= 0.05) in comparison to CT1 and CT3.However,in cirrhotic animals we observed significantly raised PP(12.6±1;+26%(Gr2), vs 10± 1; in Gr1) a marked reduction in Gr3(10.8 ±0.2;-14%) vs Gr2(p=0.01) was observed. Gr2 animals had increased SMABF (p=0.001) and PBF (p=0.001) in comparison to Gr1 and Gr3. Endothelial dysfunction assay under perfused ex-vivo condition showed improvement in endothelial activity showed by percentage change in Portal perfusion pressure (p=0.005) in Gr3&Ct3 vs Gr2&Ct2. Periportal inflammation and inflammatory cells infiltration was evidently reduced in both Ct3 and Gr3 rats vs Ct2&Gr2 respectively. Gene expression of TNF-alpha, IL-6, HMGB1,DPEP1, TLR4, RAGE were significantly upregulated in Gr2 and Ct2 (p<0.05) vs Gr3 and Ct3. Moreover, Endothelial dysfunction genes ICAM1,VCAM1,DPEP1 fold was down regulated (p<0.05) in Gr3&Ct3 vs Gr2&Ct2.Serum levels of ALT and AST and LDH were down regulated (p<0.05) in Gr3 & Ct3 vs Gr2 & Ct2.</p><p><b><i>Conclusion:</i></b> Pharmacological inhibition of HMGB1 ameliorates PP and protects vascular function during sepsis in liver cirrhosis.</p><p><b>PP-01-335</b></p><p><b>Assessing Microplastic Impact on Gastrointestinal Health: Findings from Jakarta's Cross-Sectional Study</b></p><p>Liovicinie Andarini<sup>1</sup>, Nur Hamidah<sup>1</sup>, <b>Ari Syam</b><sup>1</sup>, Pukovisa Prawiroharjo<sup>2,3</sup>, Anyelir Nielya Mutiara Putri<sup>2</sup>, Noryanto Ikhromi<sup>2</sup> and Elizabeth Divina<sup>2</sup></p><p><sup>1</sup><i>Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;</i> <sup>2</sup><i>Department of Neurology, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;</i> <sup>3</sup><i>Universitas Indonesia Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To measure microplastic exposure and its association with gastrointestinal symptoms using the Gastrointestinal Symptom Rating Scale (GSRS).</p><p><b><i>Materials and Methods:</i></b> A cross-sectional study was conducted with 439 subjects from Jakarta and surrounding areas. Participants were selected using simple random sampling from those exposed to microplastics orally and met the inclusion criteria: willingness to participate, ability to complete the questionnaire, and age between 18-59 years. Data were collected using a validated questionnaire assessing microplastic exposure and gastrointestinal symptoms, with clinical assessment through the GSRS, categorized into five syndromes: diarrhea, indigestion, constipation, abdominal pain, and reflux. Plastic usage was categorized into three levels: low (less than once a month), moderate (once a month to 6 times a week), and high (once a day or more). Statistical analysis included descriptive statistics, normality test, and Kruskal-Wallis test, followed by post-hoc Mann-Whitney tests for significant differences.</p><p><b><i>Results:</i></b> Analysis of 439 participants revealed a significant correlation between bottled water in plastic and GSRS scores for indigestion (p=0.025) and abdominal pain (p=0.030). No significant correlation was found between gallon water consumption and GSRS scores for any gastrointestinal syndrome (p>0.05). Plastic-packaged food consumption showed significant correlations with GSRS scores for diarrhea (p=0.002), indigestion (p=0.028), and constipation (p=0.034). Post-hoc analysis revealed significant differences between low vs. medium and low vs. high consumption for all significant symptoms across all groups (p<0.05).</p><p><b><i>Conclusion:</i></b> The study demonstrates significant associations between microplastic exposure and gastrointestinal symptoms, suggesting that microplastics from plastic packaging may contribute to gastrointestinal issues.</p><p><b>PP-01-339</b></p><p><b>Zonula occludens 1 as a novel diagnostic biomarker in decompensated cirrhotic patients with hepatorenal syndrome</b></p><p><b>Balasubramaniyan Vairappan</b> and Raj Kumar and Mukta Wyawahare</p><p><i>Jawaharlal Institute Of Postgraduate Medical Education And Research, Puducherry, India</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Hepatorenal syndrome (HRS) is a life-threatening complication of liver cirrhosis with renal failure. The common biomarkers to assess kidney injury have substantial limitations in cirrhotic patients. Here, we aimed to assess the circulating concentration of Zonula occludens (ZO) 1 and cystatin C in decompensated cirrhotic (DC) patients with HRS and to evaluate their diagnostic potential of HRS.</p><p><b><i>Design & Methods:</i></b> DC patients with HRS and normal healthy volunteers (n=40, for both) were recruited in the study. Serum ZO-1, cystatin C and clinical chemistry parameters were analysed.</p><p><b><i>Results:</i></b> When compared to control subjects, an increase in the concentration of ZO-1 (7.135 ± 0.283 vs 0. 788 ± 0.114; P < 0.0001) and cystatin C (2.92 ± 0.22 vs 1. 59 ± 0.04; P < 0.0001) were observed in DC patients with HRS. There was a positive correlation between serum ZO-1 and cystatin C (r=0.551, p<0.0001). Serum ZO-1 was also positively correlated with serum creatinine and MELD-Na (r=0.767, p<0.0001; r=0.841, p<0.0001, respectively).</p><p><b><i>Conclusion:</i></b> Increased level of serum ZO-1 observed in DC patients with HRS was positively correlated with cystatin c and creatinine and has improved diagnostic capability than cystatin C. Thus, this study proves the potential of ZO-1 as a valuable biomarker in DC patients on the background of HRS. Indeed, this finding should be further confirmed with a large cohort of DC patients with HRS.</p><p><b>PP-01-341</b></p><p><b>Serum cryoglobulinemia in chronic hepatitis C patients after cure by anti-viral agents</b></p><p><b>Chia-Yen Dai</b><sup>1,2</sup>, Batbold Batsaikhan<sup>3</sup>, Po-Cheng Liang<sup>1</sup>, Chung-Feng Huang<sup>1,2</sup> and Ming-Lung Yu<sup>1,2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;</i> <sup>2</sup><i>Faculty of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;</i> <sup>3</sup><i>Department of Internal Medicine, Institute of Medical Sciences, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> Cryoglobulinemia is one of the major extra-hepatic manifestations of chronic hepatitis C infection. Persistent cryoglobulinemia after completion of antiviral treatment is an important consideration of clinical management in chronic hepatitis C patients. We aimed to investigate the occurrence of serum cryoglobulinemia in chronic hepatitis C patients without cryoglobulinemia at the initiation of antiviral treatment after cure.</p><p><b><i>Methods:</i></b> Totally 776 patients without cryoglobulinemia were assessed for serum cryoglobulinemia after completion of anti-HCV treatment. Serum cryoglobulinemia precipitation was assessed both initiation and completion of the treatment and analyzed for the clinical, and laboratory factors associated with chronic hepatitis C.</p><p><b><i>Results:</i></b> There were 118 patients were checked for serum cryo-precipitation after completion of the treatment and 8 patients (4.6%) were positive for serum cryoglobulinemia. Patients who became positive cryoglobulinemia had a higher proportion of liver cirrhosis patients (4/50%, p=0.033) and other organ cancer patients (5/62.5%, p=0.006) than patients who remained no sign of cryoglobulinemia after treatment. In multivariate analysis, liver cirrhosis (Odds Ratio [OR]–17.86, 95% Confidence Interval [95% CI]– 1.79-177.35, p = 0.014) and other organ cancer (OR-25.17 95% CI– 2.59-244.23, p = 0.005) were independently and significantly associated with positive cryoglobulinemia 3 months after antiviral treatment.</p><p><b><i>Conclusions:</i></b> A 6.7% prevalence of cryoglobulinemia three months after the antiviral DAA therapy was observed. Liver cirrhosis and other organ cancer were independently and significantly associated with positive cryoglobulinemia after antiviral treatment. Further investigation into the causes of positive cryoglobulinemia after DAA antiviral therapy is necessary.</p><p><b>PP-01-344</b></p><p><b>Study of overall survival and factors affecting outcomes in chronic HCV patients undergoing liver transplantation</b></p><p><b>Kongpob Yongrattanakit</b></p><p><i>Khonkaen University, Khonkaen, Thailand</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>-Primary outcome:</i></b> overall survival of LT recipients with chronic HCV infection</p><p><b><i>-Secondary outcomes:</i></b> Factors affecting overall survival, rate of HCV, cirrhosis, and HCC recurrence.</p><p>-16-year-period, medical records of adult LT recipients with HCV infection, transplanted between January 2008 and May 2023, were reviewed. Recipients with only anti-HCV positivity and lacking confirmation of HCV VL before transplantation were excluded. Patient medical records were abstracted to gather information. Survival curves were generated using Kaplan-Meier method and compared using log-rank test. Univariate Cox proportional hazard models were employed to calculate hazard ratios for variables associated with death.</p><p>-The medical records of 81 adult liver transplantation recipient with chronic HCV transplanted at Srinagarind Hospital. HCV recurrence was observed in 98.6% of all cases. Cirrhosis recurrence occurred in 4.9%, HCC recurrence was noted in 7.4%. 5-year overall survival rate for LT recipients was 75.3%. For factors affecting outcomes, HCC recurrence (HR, 26.55; 95% CI; P < 0.001) and cirrhosis recurrence (HR, 9.05; P = 0.008) were found to be correlated with increased risk of mortality. Both HCV treatment after LT (HR, 0.09; P < 0.001) and achieving SVR at final follow-up visit (HR, 0.13; P < 0.001) were significantly associated with reduced risk of death.</p><p>-Over 16-year peroid, 5-year overall survival rate for LT recipients with HCV infection was 75.3%. Recurrence of HCC and cirrhosis were significant risk factors for reducing overall survival. Conversely, LT patients who underwent HCV treatment and achieved SVR status at final follow-up visit demonstrated good factor to improve overall survival.</p><p><b>PP-01-353</b></p><p><b>An adrenal pseudocyst masquerading as a pancreatic neoplasm – A case report</b></p><p><b>Padmakumara Udapamunuwa</b>, Melan Jayasinghe, Nayana Ratnayake and Mohammed Mikram and Janusha Udapamunuwa</p><p><i>National Hospital Kandy, Kandy, Sri Lanka, <sup>2</sup>Department of Medicine, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka</i></p><p>Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Adrenal pseudocysts are seldom encountered, non-functional cysts of the adrenal gland, located retroperitoneally. Accurate preoperative diagnosis is challenging due to their resemblance of other cystic lesions, especially those of pancreatic origin. This report discusses the diagnostic and surgical approach to a large adrenal pseudocyst initially misdiagnosed as a pancreatic neoplasm.</p><p><b><i>Case description:</i></b> A 36-year-old, ASA-1 female presented with vague back pain and non-specific abdominal discomfort. Ultrasound scan revealed a large unilocular cyst over the body and tail of the pancreas. Rest of the investigations were normal. A contrast enhanced computed tomography scan identified a 13x11x10 cm cyst with no communication to the pancreatic ducts. Initial diagnostic impressions included pancreatic pseudocyst or mucinous pancreatic neoplasm. Following the multidisciplinary team discussion, the patient was prepared for a distal pancreatectomy with possible splenectomy. Intraoperatively, a large retroperitoneal cystic mass was found. It was separate from the pancreas and located on the superior pole of the left kidney. The mass was resected en bloc. Histology of the specimen revealed an adrenal pseudocyst.</p><p><b><i>Discussion</i></b>: Adrenal pseudocysts are benign cortical cysts devoid of an epithelial lining. They are rare, with only about 250 cases being reported previously. Due to their proximity to the other retroperitoneal organs, radiological delineation of the lesion is difficult. This case highlights the importance of considering an adrenal pseudocyst as an important differential diagnosis for retroperitoneal cystic lesions. If diagnosed correctly, simple cystectomy can be planned and this will avoid unnecessary preoperative preparations.</p><p>Graph/Image/Table</p><p><b>PP-02-001</b></p><p><b>Multiple colonic diverticular bleeding: a case report</b></p><p><b>Josephine Amanda Setiadi</b>, Bradley Jimmy Waleleng, Fandy Gosal, Luciana Rotty, Jeanne Winarta and Andrew Waleleng</p><p><i>Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia, Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Colonic diverticular bleeding is the primary cause of acute lower gastrointestinal bleeding as a complication of diverticulosis.<sup>1</sup> Less than 5% of patients with diverticulosis presents with bleeding. In Asian countries, diverticula are predominant in the right-sided colon, in contrast to the sigmoid colon in Western countries.<sup>2</sup></p><p><b><i>Case Illustration:</i></b> A 63-year-old woman was referred to our gastroenterology division with complaints of moderate volume hematochezia since two days before admission. The patient denied abdominal pain. She reported a previous history of hematochezia five years ago which resolves spontaneously and hypertension treated with amlodipine 5mg daily since five years ago. Her vital signs were normal. On physical examination, the patient appeared anaemic and abdominal examination was unremarkable. Digital rectal examination revealed internal haemorrhoid and bloody stool. Blood tests revealed hemoglobin of 10.4g/dL. Colonoscopy revealed active bleeding from multiple ascending colon diverticulum, proctosigmoiditis, and internal hemorrhoid. The bleeding was controlled using haemostatic clips. The patient condition improved and discharged without complications.</p><p><b><i>Discussion:</i></b> We documented a case of diverticular bleeding in a 63-year-old female patient. The likelihood of diverticular bleeding increases with advancing age.<sup>2</sup> Diverticular bleeding usually stops spontaneously but in this case, continued more than two days. A previous history of hematochezia and hypertension might contribute to rebleeding.<sup>3</sup> Right-sided diverticular disease is associated with higher incidence of bleeding complications and colonoscopy revealed multiple ascending colon diverticular bleeding.<sup>1</sup> Haemostasis was achieved using haemostatic clips which is known to be effective and had a low risk of rebleeding.<sup>4</sup> The patient recovered well after 1 week follow-up.</p><p><b>PP-02-002</b></p><p><b>Endoscopic therapy with high concentration dextrose: a comeback with a new twist</b></p><p><b>Shahreedhan Shahrani</b><sup>1</sup>, Bo Shen<sup>2</sup> and Ida Normiha Hilmi<sup>1</sup></p><p><sup>1</sup><i>University Malaya Medical Centre, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Center for Inflammatory Bowel Diseases, University Irving Medical Center/ NewYork-Presbyterian/Columbia, New York, USA</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Diffuse non-variceal, non-arterial bleeding, albeit common, can be quite an endoscopic challenge. Standard methods of endoscopic therapy, may not be effective due to the inability to pinpoint an exact location of bleeding. Intra-lesional high concentration dextrose has been used in managing variceal bleeding historically. There have been limited reports of its use in managing other causes of diffuse bleeding.</p><p><b><i>Case description:</i></b> We report a case of a 61-year-old gentleman, who presented with a history of altered bowel habit and an acute history of per rectal bleeding. He was hemodynamically stable; however, hemoglobin showed a reduction of 3g/L. A colonoscopy was performed, revealing a fungating, circumferential, friable and bleeding mass 10cm from anal verge extending for approximately 10cm. There was diffuse, active oozing of blood from the lesion, even prior to multiple biopsies being taken for histopathological examination (HPE). A decision was made to use high concentration dextrose to spray on the diffuse mucosal bleeding site, after specimen biopsies were taken. A through-the-scope spray catheter (Olympus, tubing length 1650mm) was used to spray 12ml of dextrose 50%. Complete hemostasis was achieved. There were no more further drop in hemoglobin levels or further episodes of acute PR bleeding until his semi-elective surgery for tumor resection was arranged.</p><p><b><i>Discussion:</i></b> The consideration behind using hypertonic glucose for diffuse mucosal GI bleeding is similar to that of its’ use in gastric varices, through osmotic dehydration. Endoscopic therapy with high concentration dextrose may be considered as an alternative method for managing diffuse non-variceal, non-arterial bleeding.</p><p><b>PP-02-003</b></p><p><b>A case of esophagectomy and subtotal-stomach reconstruction for esophago-mediastinal fistula after omentoplasty for graft infection</b></p><p><b>Rai Shimoyama</b><sup>1</sup>, Takaaki Murata<sup>1</sup>, Yuma Suno<sup>1</sup>, Naoko Isogai<sup>2</sup>, Jun Kawachi<sup>2</sup>, Daisuke Hama<sup>1</sup>, Yusuke Gunji<sup>1</sup>, Motoki Nagatsuka<sup>1</sup>, Takeshi Yamabe<sup>1</sup> and Kenichiro Noguchi<sup>1</sup></p><p><sup>1</sup><i>Shonan Kamakura General Hospital, Kamakura, Japan;</i> <sup>2</sup><i>Hayama Heart Center, Hayama, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> We report a case of a patient who underwent subtotal stomach reconstruction with esophagectomy for esophago-mediastinal fistula after omentoplasty for graft infection after graft replacement for thoracic aortic dissection.</p><p><b><i>Case Report:</i></b> The patient was a 48-year-old woman after aortic arch replacement and open stent implantation for thoracic aortic dissection. The patient underwent omentoplasty 1 year 4 month after the initial surgery and re-total arch replacement 2 year 1 month after the initial surgery for graft infection. Patient was diagnosed as esophago-mediastinal fistula 1 month after the last operation, but fistula closure with Over-The-Scope-Clip or esophageal stent were all unsuccessful and the infection was uncontrolled. Subtotal esophagectomy was performed 3 years 7 months after the initial operation. As the right gastroepiploic artery was devided at the time of the omentoplasty, subtotal stomach reconstruction was performed to preserve the blood flow on the lesser curvature. The postoperative course was good, without recurrence of fistula or graft infection.</p><p><b><i>Discussion:</i></b> In this case, conservative treatment was unsuccessful and esophagectomy had to be chosen. However, because the right gastroepiploic artery was dissected at the time of surgery for omentoplasty, there was a high risk of gastric tube necrosis with conventional gastric tube reconstruction. Colonic reconstruction was considered, but due to the complexity of the surgical technique, reconstruction by subtotal stomach reconstruction was chosen.</p><p>In cases where blood flow on the greater curvature side is expected to be insufficient, subtotal gastric reconstruction with preservation of the lesser curvature may be an effective option.</p><p><b>PP-02-004</b></p><p><b>Emphysematous Gastritis with Polymicrobial Etiology in a Gouty Arthritis Patient</b></p><p><b>Jose Luis Matthias Sollano</b> and Gerardo Pedregosa</p><p><i>Makati Medical Center, Makati, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Emphysematous gastritis (EG) is a rare condition characterized by gas accumulation within the gastric wall. This case report presents a patient with EG from a polymicrobial infection with an acute kidney injury complicated by gouty arthritis.</p><p><b><i>Case Description:</i></b> A 53-year-old male with gouty arthritis presented with severe abdominal pain, chills, diarrhea, and vomiting. Ancillaries showed leukocytosis at 12x10^3/uL and creatinine 2.18mg/dL. Imaging revealed air locules at the posterior wall of the gastric fundus and tubular air-filled foci in certain hepatic segments and vascular structures. The patient was given antibiotics, hydration and placed on NPO with an NGT. Endoscopy identified an infected gastric mass and ulcer, from which Neisseria flava/perflava/subflava, Serratia marcescens, and Streptococcus salivarius were cultured with resistance. Broad-spectrum antibiotics, total parenteral nutrition and supportive care was given. During the admission, the gouty arthritis was in flare at the left knee and right foot which was treated with IV corticosteroids. The patient had a repeat of the imaging and endoscopy, both showing significant improvement in the initial findings. He was subsequently discharged stable and completed antibiotics for 14 days.</p><p><b><i>Discussion:</i></b> With high mortality rates of about 60-80%, the importance of comprehensive diagnostic workup and appropriate management is crucial to the survival of patients with EG. This case, complicated by a polymicrobial nature and ongoing flare of gouty arthritis, emphasizes early recognition and management are crucial for optimal patient outcomes. Further studies are needed to elucidate the pathophysiology of this rare condition and to identify potential risk factors.</p><p><b>PP-02-005</b></p><p><b>A Puzzling Case of Recurrent Fever, Jaundice, and Tea-Colored Urine in a 32-Year-Old Male</b></p><p><b>Jose Luis Matthias Sollano</b></p><p><i>Makati Medical Center, Makati, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> This case report details the diagnostic journey of a 32-year-old male with a perplexing three-year history of recurrent fever, jaundice, tea-colored urine, and occasional acholic stools, each episode spontaneously resolving.</p><p><b><i>Case Description:</i></b> Exhaustive investigations initially yielded inconclusive results, challenging the conventional diagnostic paradigm. Subsequent evaluation, including liver function tests and imaging studies, revealed subtle abnormalities, prompting a reevaluation of potential infectious etiologies. The breakthrough in diagnosis came with a positive result in the QuantiFERON®-TB Gold test, unveiling the presence of Tuberculosis (TB) as an underlying cause for the recurrent symptomatic episodes. Additional confirmation came along with the resolution of the symptoms when Anti-Koch’s treatment was initiated.</p><p><b><i>Discussion:</i></b> This report discusses the challenges faced in diagnosing Hepatic Tuberculosis, emphasizing the need for heightened clinical suspicion and a comprehensive diagnostic approach. It also underscores the importance of timely identification and initiation of appropriate anti-tubercular therapy to prevent further complications and improve patient outcomes.</p><p>This case serves as a poignant reminder of the diverse clinical presentations of tuberculosis, urging clinicians to maintain a broad differential diagnosis and consider rare manifestations in the pursuit of resolving diagnostic conundrums associated with recurrent fever, jaundice, and tea-colored urine.</p><p><b>PP-02-006</b></p><p><b>Olmesartan induced enteropathy masquerading as celiac disease</b></p><p><b>Pulkit Sondhi</b>, Neha Berry, Amrish Sahney, Manav Wadhawan, Rameez Raja Najar and Ajay Kumar</p><p><i>Blk Max SuperspecialityHospital New Delhi, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Backround:</i></b> Olmesartan, an antihypertensive medication has been rarely associated with sprue like enteropathy clinically presenting with chronic diarrhea, weight loss and villous atrophy on biopsy.</p><p><b><i>Case Description:</i></b> We describe a 67 year old female known case of hypertension on Olmesartan for many years presented with recurrent episodes of intermittent diarrhea complicated with dyselectrolytemia and renal failure. Though patient had endoscopic evidence suggestive of celiac disease (CeD) with equivocal biochemical tests and Feature of Ulcerative colitis (UC) on colonoscopy. Patient was on strict Gluten free diet (GFD) for one and half year with no improvement and also received oral Steroids and immunomodulatory therapy/ small molecules for possible steroid dependent UC. Despite receiving treatment for both CeD and UC, patient continued to have intermittent intractable symptoms. After stopping olmesartan considering as possible culprit agent, patient showed drastic improvement in symptoms thus reinforcing the diagnosis of Olmesartan induced Enteropathy (OIE).</p><p><b><i>Discussion:</i></b> Clinicians and patients should be aware that olmesartan can cause an enteropathy clinically and histopathologically similar to celiac disease as well as a colopathy similar to microscopic colitis. Failure to recognize olmesartan-induced enteropathy may result in patients continuing on a medication that is injurious to the gastrointestinal tract or embarking on an unnecessary and expensive medical evaluation, frequently with steroid use, with both options disrupting quality of life. Time to recognition of this entity is often prolonged, suggesting the need for increased awareness in both patients and clinicians.</p><p><b>PP-02-007</b></p><p><b>Splenic Abscess: An Unexpected Clue to Underlying Malignancy</b></p><p><b>Christine Marianne Sy</b>, Rial Juben De Leon and Rona Marie Lawenko</p><p><i>De La Salle University Medical Center, Dasmariñas City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Splenic abscess is a relatively uncommon medical condition, with reported incidence rates ranging from 0.05% to 0.7%. This type of abscess typically occurs in individuals who are immunocompromised. This is a case of a 71 year old female presenting with splenic abscess masking a malignancy.</p><p><b><i>Case Description:</i></b> A 71 year old Filipino female with uncontrolled diabetes presented with fever and left upper quadrant pain. Workup showed leukocytosis with neutrophilic predominance and imaging showed splenic abscess. Broad-spectrum antibiotics were administered, and percutaneous drainage was done. Culture of abscess showed Escherichia coli. Post drainage, there were persistent fever and left upper quadrant pain. Repeat CT scan then showed a tumor measuring 10.9 x 7.5 x 10.9 cm in the inferior aspect of the spleen with mass effect. A more definitive management is offered based on the biopsy results.</p><p><b><i>Discussion:</i></b> Splenic abscess is more often secondary to hematogenous bacterial seeding. Only a few reported cases were documented to be from a tumoral necrosis. Splenic abscess may be clinically diagnosed based on the triad of fever, left upper quadrant pain, and leukocytosis. However, imaging is still essential. Treatment guidelines on splenic abscess are limited due to its rarity. Empiric broad-spectrum antibiotics are initiated once splenic abscess is suspected. Splenic abscess alone may be managed conservatively with percutaneous drainage and aggressively with splenectomy, with no difference in mortality based on studies. However, in the background of malignancy, treatment options may vary.</p><p><b>PP-02-008</b></p><p><b>A case of fractured and migrated Fully Covered SEMS removed by peroral cholangioscopy.</b></p><p><b>Shinya Tajima</b>, Ken Ito, Michihiro Saitou, Masataka Kurihara, Yuto Yamada, Yousuke Okamoto and Manabu Watanabe</p><p><i>Toho University Ohashi Medical Center, Tokyo, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Fully Covered Self-Expanding Metal Stents (FC-SEMS) are recommended as a treatment option for benign bile duct stricture associated with chronic pancreatitis.</p><p>Complications such as fracture and migration of FC-SEMS are rare, and there is no standardized treatment for these issues.</p><p><b><i>Case Description:</i></b> A 50-year-old man with chronic pancreatitis and benign bile duct stricture periodically underwent plastic stent (PS) replacement. Due to a lack of improvement, FC-SEMS (M-Intraductal; Medico's Hirata Inc., Osaka, Japan) was placed and scheduled for stent removal after four months. However, 3.5 months after stent placement, he developed acute cholangitis. Abdominal CT revealed a fractured FC-SEMS and dilated intrahepatic bile ducts. Urgent ERCP revealed that the FC-SEMS had migrated into the proximal bile duct. The FC-SEMS retrieval was unsuccessful, so we temporarily placed a PS, and scheduled the retrieval for the next session using peroral cholangioscopy (POCS). The fractured FCSEMS was kept in a clear view using POCS and successfully removed using grasping forceps (Spy Bite MAX, Boston Scientific).</p><p><b><i>Discussion:</i></b> POCS was effective for troubleshooting the retrieval of FC-SEMS. These techniques sometimes result in a successful outcome for rare fractured and migrated cases.</p><p><b>PP-02-009</b></p><p><b>A Little Old Lady's Hernia Causing Small Bowel Obstruction</b></p><p><b>Erika Johanna Tañada-Escanlar</b></p><p><i>UST Hospital, manila, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Obturator hernia nicknamed as little old lady's hernia is an extremely uncommon pelvic and intra-abdominal hernia with an incidence of 0.07-1.4% affecting mostly emaciated elderly woman. It has the highest morbidity and mortality rates of all abdominal wall hernias and a significant cause of intestinal obstruction thus early recognition is paramount. We report a case of a gaunt 75-year-old woman who presented with small bowel obstruction due to incarcerated obturator hernia, successfully managed surgically.</p><p><b><i>Case Description:</i></b> A gaunt 75-year-old woman presented with 7 days periumbilical abdominal pain along with bilious vomiting and abdominal distension. Clinical examination revealed abdominal distension, hyperactive bowel sounds and absence of tenderness. She has a surgical scar for two cesarean sections. Biochemical parameters were normal. Plain radiograph of abdomen revealed dilated small bowels with multiple air fluid levels. A computed tomography (CT) scan revealed herniation of the distal small intestinal loops into the right inguinal region through the obturator foramen.The patient underwent an exploratory laparotomy and a mesh-plug hernioplasty. During follow-up, there was no evidence of recurrence or complications.</p><p><b><i>Discussion:</i></b> Obturator hernia refers to the herniation of intra-abdominal contents through the obturator canal causing 0.2% to 1.6% of all small bowel obstructions. In an emaciated elderly female presenting with intestinal obstruction, obturator hernia is an important differential diagnosis. Early recognition is essential in order to provide optimum surgical treatment to significantly reduce the mortality and morbidity rates.</p><p><b><i>Keywords:</i></b> Hernia, Obturator, herniorrhaphy, Laparotomy, Abdominal Pain, Abdominal Wall, Aged, Intestinal Obstruction, Intestine, Small,</p><p><b>PP-02-010</b></p><p><b>Defying the Conventional Paradigm: A Rare Case of Spontaneous Resolution in Chronic Intestinal Pseudo-Obstruction</b></p><p>Marcella Adisuhanto<sup>1,2</sup>, Enrico Christian<sup>3</sup>, <b>Surya Sinaga Immanuel</b><sup>1</sup>, Alvin Edwin Wiyono<sup>1</sup> and Riki Tenggara<sup>1</sup></p><p><sup>1</sup><i>Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Atma Jaya Academic Hospital, North Jakarta, Indonesia;</i> <sup>3</sup><i>School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Chronic intestinal pseudo-obstruction (CIPO) is a rare and perplexing gastrointestinal motility disorder characterized by symptoms mimicking mechanical bowel obstruction without detectable blockage. Typically, CIPO leads to significant morbidity, including malnutrition and a diminished quality of life. Spontaneous resolution of CIPO is exceedingly rare, making it of significant clinical interest.</p><p><b><i>Case Description:</i></b> We present a compelling case of a 30-year-old female with a seven-year history of CIPO, characterized by recurrent spontaneous episodes of abdominal distension and pain, resolving within minutes to hours. Remarkably, despite the chronicity, the patient maintained stable nutritional status and carried out her daily activities without significant disruption. Extensive diagnostic evaluations, including ultrasonography, barium follow-through, gastroscopy, colonoscopy, and abdominal computed tomography scans, revealed no structural abnormalities. However, an incidental Helicobacter pylori infection was found and treated without symptomatic improvement. Notably, symptom frequency and severity correlated with her menstrual cycle and began subsiding two years after completing medical education, eventually leading to a complete resolution.</p><p><b>PP-02-011</b></p><p><b>Lafora-like Inclusion Bodies are associated with Polypharmacy</b></p><p><b>Leia Teo</b></p><p><i>MOHH, Changi General Hospital, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The differentials for the presence of lafora like inclusion bodies are increasingly diverse - ranging from fatal neurological afflictions such as Lafora disease to more common liver-related conditions</p><p>like hepatitis B infection. With growing access to healthcare, polypharmacy is also a crucial differential to consider.</p><p><b><i>Case Description:</i></b> Mr CYS is a 69yo Chinese male with polypharmacy whose liver biopsy revealed lafora like inclusion bodies. A thorough review of his extensive medical history, medications and histological slides was performed. The differentials for his histological findings of interest were systemically ruled out through further investigations and clinical assessment by relevant specialists.</p><p><b><i>Discussion:</i></b> This paper supports the growing evidence that Lafora like inclusion bodies are associated with polypharmacy - which includes chemotherapy, supplements and common medications like painkillers. Recognising this avoids the mislabelling of serious conditions and misguided treatment. Additionally, as such inclusion bodies may be detrimental to cells, reduction of polypharmacy should be considered.</p><p><b>PP-02-012</b></p><p><b>Addressing the Treatment Gaps for Severe Crohn's Disease in SEA: A Case of Infliximab-Associated Sepsis</b></p><p><b>Tinpawee Thongkongthun</b><sup>1</sup>, Soonthorn Chonprasertsuk<sup>2</sup>, Ratha Korn Vilaichone<sup>2</sup>, Natsuda Aumpan<sup>2</sup>, Pongjarat Nunanan<sup>2</sup>, Bubpha Pornthisarn<sup>2</sup>, Sith Siramolpiwat<sup>2,3</sup>, Navapan Issariyakulkarn<sup>2</sup>, Patommatat Bhanthumkomol<sup>2</sup>, Napakul Siripen<sup>2</sup>, Virunpat Vilaichone<sup>1</sup> and Arti Wongcha Um<sup>3</sup></p><p><sup>1</sup><i>Faculty of Medicine, Chulabhorn International College of Medicine (CICM), Thammasat University, Pathumthani, Thailand;</i> <sup>2</sup><i>Department of Medicine, Gastroenterology Unit, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand;</i> <sup>3</sup><i>Department of Medicine, Chulabhorn International College of Medicine (CICM) at Thammasat University, Pathumthani, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> This paper highlights the challenges and limitations in treating severe Crohn's disease in Thailand, particularly focusing on the use of biologic therapy in the presence of occult HBV infection.</p><p><b><i>Case Description:</i></b> A case report of a 67-year-old Thai male with a complex medical history. The patient was diagnosed with stricture colonic Crohn's disease refractory to budesonide and treated with infliximab and azathioprine, leading to severe complications including drug-induced bone marrow suppression, PCP pneumonia, occult HBV infection, and severe sepsis. Despite aggressive treatment, the disease progressed. In Thailand, only a few biological treatments are available and are extremely limited under universal coverage, presenting significant risks to this patient.</p><p><b><i>Discussion:</i></b> This case underscores the rarity and complexity of severe Crohn's disease in Southeast Asia, where the prevalence is low and treatment guidelines are limited. The use of infliximab necessitated antiviral prophylaxis due to the increased risk of HBV reactivation. Detailed endoscopic findings showed progressive disease with significant clinical challenges. The need for region-specific treatment protocols and the importance of screening for latent infections before initiating biologic therapy are emphasized. Comparisons with existing literature and guidelines highlight gaps and propose potential improvements. This case report illustrates the critical need for tailored IBD management guidelines in Thailand and similar regions. Awareness of the risks associated with biologic therapies, particularly in patients with latent infections, is essential. Proper preparation and individualized treatment plans are necessary to improve outcomes and reduce severe complications.</p><p><b>PP-02-013</b></p><p><b>Young Woman with Autoimmune Hepatitis: A Rare Case</b></p><p><b>Lioni Tjoeng</b> and Muhammad Yamin Lubis</p><p><i>Department of Internal Medicine, EMC Sentul Hospital, Bogor, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Autoimmune hepatitis (AIH) is a heterogeneous immune-mediates liver disease that, in most cases, has effective treatment when diagnosed promptly and treated judiciously. Clinically, patients with AIH are characterized by raised serum ALT activity, hypergammaglobulinemia, non-organ-specific autoantibodies, & a chronic relapsing hepatitis, associated with a plasma cell hepatic infiltrate.</p><p><b><i>Case Description:</i></b> A 22-year-old asian woman came with a complaint of maculopapular rash all over her body since 1 month ago. The rash can be itchy and uncomfortable. Other complaints include weakness, hair loss and weight loss. She also never consumed alcohol and took medication. Laboratory values revealed elevated AST(407 U/L), ALT(415 U/L), Gamma GT(178 U/L), ALP(227 U/L), & Globulin(4 g/dL). The SMA was positive, and ANA IF was speckled pattern with titre 1:100. Meanwhile, the results of AMA, anti LKM-1,SLA/LP,LC-1, ANA profile, hbsag, anti-hcv, & anti-hav IgM were all negative. The result of MRCP was splenomegaly. Liver biopsy could not be performed due to patient refusal. Treatment with prednisone 60 mg/day was initiated and the drug dose was reduced slowly according to the patient's clinical condition. Her condition began to improve, skin lessions on her skin disappeared and her lab test results returned to normal.</p><p><b><i>Discussion:</i></b> In determining the diagnosis and treatment of patients, we use the IAIHG scoring system with a result of 13 (possible diagnosis of AIH) because there are examinations such as liver biopsy that cannot be carried out. The aim of this case report is to increase awareness of the complications of AIH and prevent irreversible morbidity.</p><p><b>PP-02-014</b></p><p><b>Rothia Mucilaginosa causing spontaneous bacterial peritonitis</b></p><p><b>Muhammad Usama</b> and Athesham Zafar</p><p><i>Walsall Healthcare Nhs Trust, Walsall, United Kingdom</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>55-year-old male presented with worsening abdominal pain, distension, and low-grade fever over the past few weeks. He had a history of decompensated cirrhosis secondary to alcohol. The patient denied any recent travel, intravenous drug use, or recent hospitalizations.</p><p>On examination, the patient appeared ill with low-grade fever (38.0°C) and was hemodynamically stable. His abdomen was distended, with localized tenderness in the lower quadrants. Fluid wave was absent, but ultrasound revealed loculated ascitic pockets. There was no evidence of hepatic encephalopathy or jaundice.</p><p>Initial laboratory tests showed mildly elevated white blood cell count, with a predominantly neutrophilic response. Liver function tests showed elevated transaminases and hypoalbuminemia. Ascitic fluid analysis revealed an elevated total protein concentration and low serum-ascites albumin gradient (SAAG), suggestive of secondary peritonitis rather than uncomplicated cirrhotic ascites. A computed tomography (CT) scan showed no evidence of perforated viscus.</p><p>Ascitic fluid cultures were positive for *Rothia mucilaginosa*, an unusual finding typically associated with immunocompromised states. Blood cultures were negative.</p><p>The patient was diagnosed with loculated ascites secondary to cirrhosis, complicated by infection with *Rothia mucilaginosa*. Broad-spectrum antibiotics, including vancomycin, were initiated while awaiting sensitivity results, and a percutaneous catheter was placed for drainage of the loculated fluid collections. He was given albumin infusions with careful fluid management.</p><p>This case highlights the importance of considering atypical organisms in infected ascitic fluid, particularly in patients with cirrhosis and complex ascitic presentations like loculated ascites. Early recognition and targeted treatment are crucial for managing these infections and preventing further complications.</p><p><b>PP-02-015</b></p><p><b>The Role Of Manometry in Refractory GERD</b></p><p><b>Amanda Pitarini Utari</b>, Jessica Wijaya, Liovicinie Andarini and Ari Fahrial Syam</p><p><i>FKUI, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> High-resolution manometry (HRM) is crucial for patients suspected of gastroesophageal reflux disease to rule out conditions with similar findings, such as achalasia and rumination syndrome. Despite its importance, manometry availability is limited in many parts of Indonesia. This study highlights the vital role of manometry in diagnosing and managing persistent GERD.</p><p><b><i>Case Description:</i></b> A 37-year-old man presented with a two-month history of heartburn and difficulty swallowing, along with nausea when eating solid food, and feeling sensations of food being lodged in his esophagus. He had a history of GERD diagnosed three years prior and hematochezia due to an anal lump. Despite weight loss, his physical examination was unremarkable. Esophagogastroduodenoscopy (EGD) revealed grade A esophagitis and moderate gastritis with a differential diagnosis of achalasia, and biopsies showed no significant findings. A pH impedance assessment led to a diagnosis of refractory GERD, with a DeMeester Score of 28.32. Initial manometry indicated ineffective esophageal motility. The patient was treated with Rabeprazole, Domperidone, Rebamipide, and Ondansetron. Follow-up manometry results were normal.</p><p><b><i>Discussion:</i></b> EGD has low diagnostic accuracy for GERD, as only 30% of patients have erosive reflux disease. Manometry plays a crucial role in excluding other diagnoses in GERD patients with normal mucosa or nonspecific esophagitis findings. Patients with GERD may be found with Esophageal-Gastro Junction (EGJ) incompetence as well as ineffective esophageal motility (IEM). Severe IEM serves as a key indicator of a more serious form of GERD.</p><p><b>PP-02-016</b></p><p><b>Upadacitinib induction and maintenance therapy in severe Crohn’s disease: A case report</b></p><p><b>Nicholas Wan</b><sup>1,2</sup>, Edward Young<sup>1,2</sup>, Arvind Rajagopalan<sup>1,2</sup>, Barbra Andrin<sup>1</sup> and Dharshan Sathananthan<sup>1,2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Lyell Mcewin Hospital, Elizabeth Vale, Australia;</i> <sup>2</sup><i>Adelaide Medical School, University of Adelaide, Adelaide, Australia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Severe Crohn’s colitis can be difficult to treat medically and can be associated with significant morbidity. We present a case of Upadacitinib to treat severe Crohn’s colitis.</p><p><b><i>Case Description:</i></b> A 34-year-old male with a past medical history of ulcerative colitis presented with acute severe colitis. He was passing 9 bloody stools daily with a CRP of 68, Hb of 104, HR of 109 and febrile to 38.4°C. Gastrointestinal ultrasound (GIUS) demonstrated active inflammation of the sigmoid, descending and transverse colon characterised by bowel wall thickening, mesenteric hypertrophy and increased doppler signal (Image D, E, F). Flexible sigmoidoscopy revealed sparing of the rectum (Image A) with severe inflammation of the sigmoid colon (Image B, C) characterised by deep ulceration and spontaneous bleeding. The diagnosis was revised to severe Crohn’s disease and the patient was commenced on IV hydrocortisone and Infliximab. He failed to respond clinically and on day 3 of admission chose to proceed with commencement of Upadacitinib to avoid surgery. He was commenced on Upadacitinib 45mg daily and demonstrated a rapid clinical and biochemical response within 3 days (2 stools daily with no blood and normalisation of CRP). 8 weeks post admission, he continued to remain symptom free and repeat GIUS confirmed transmural healing (Image, G, H, I).</p><p><b><i>Discussion:</i></b> This case report contributes to the growing evidence of Upadacitinib as a rapidly acting salvage treatment for patients with severe colitis who have failed rescue therapy with Infliximab.</p><p><b>PP-02-017</b></p><p><b>FUAS combined with ICIs in the treatment of solid tumor with liver metastasis:2 case reports</b></p><p><b>Ting Wang</b></p><p><i>Chongqing University Cancer Hospital, Chongqing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> FUAS is a local non-invasive therapy that precisely focuses the ultrasound beam on a specific target, so that high energy can be concentrated on a very small focus inside human body, where the tissue can be ablated. At present, it has been used to treat unresectable primary liver tumors and liver metastases, and it is well tolerated even in patients with poor Child-Pugh score. Compared with TACE and other methods, the treatment of liver metastases can further block the portal vein blood supply of liver metastases, and may have a more positive impact on the immune microenvironment than radiotherapy.</p><p><b><i>Case Descripition:</i></b> This case report describes two patients with liver metastases from advanced solid tumors, who both used FUAS as a local treatment for liver metastases or primary lesions. One case was treated with gemcitabine based systemic chemotherapy combined with PD-1 inhibitor; another patient could not tolerate the side effects of chemotherapy, so he chose anti angiogenic drugs combined with PD-1 inhibitors for treatment. FUAS was performed at the beginning of treatment, when the disease progression was identified or systemic drug resistance occurred. The results showed that the tumor growth was effectively suppressed.</p><p><b><i>Discussion:</i></b> During the treatment of these two patients, it was found that FUAS could be used as local treatment measure combining PD-1 inhibitor. The treatment itself did not show obvious side effects, nor did it increase the side effects of other systemic drugs.</p><p><b>PP-02-018</b></p><p><b>Malignant Gastric Tumor After Helicobacter Pylori Infection: A Case Series</b></p><p><b>Franzeska Anna Dewi Mursita Widinartasari</b><sup>1</sup>, Cecilia Oktaria Permatadewi<sup>1</sup>, Agung Prasetyo<sup>1</sup>, Didik Indiarso<sup>1</sup>, Hesti Triwahyu Hutami<sup>1</sup>, Erik Prabowo<sup>2</sup>, Abdul Mughni<sup>2</sup>, Ahmad Fathi Fuadi<sup>2</sup>, Udadi Sadhana<sup>3</sup>, Ika Pawitra Miranti<sup>3</sup>, Faiza Rizky Aryani Septarina<sup>3</sup>, Dik Puspasari<sup>3</sup>, Meira Dewi Kusuma Astuti<sup>3</sup>, Hirlan Hirlan<sup>1</sup> and Hery Djagat Purnomo<sup>1</sup></p><p><sup>1</sup><i>Departments of Internal Medicine, Division of Gastroenterohepatology, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;</i> <sup>2</sup><i>Departments of Digestive Surgery, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;</i> <sup>3</sup><i>Departments of Anatomic Pathology, Faculty of Medicine, Diponegoro University, Dr. kariadi Hospital, Semarang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Helicobacter pylori has increasingly drawn attention for its potential link to the development of malignant tumors. In Indonesia, where H. pylori infection rates are low (10.4-11.5%) and gastric cancer incidence is correspondingly low (ASR 1.22/100,000), we present two cases of H. pylori-positive patients who developed gastric cancer.</p><p><b>Case Description:</b></p><p><b><i>Case 1:</i></b> A 35-year-old woman presented with severe vomiting due to gastric outlet obstruction from pyloric stricture linked to H. pylori infection. After unsuccessful medical management, she underwent balloon dilatation followed by Roux-en-Y Gastrojejunostomy. Six months post-surgery, she developed acute cholangitis, requiring laparotomy, which uncovered a pyloric antrum mass with reactive lymphoid hyperplasia. Subsequently, she returned with ileus, prompting another laparotomy that revealed severe adhesions. Omentum biopsy revealed a malignant round cell tumor, possibly poorly differentiated adenocarcinoma or non-Hodgkin's lymphoma.</p><p><b><i>Case 2:</i></b> A 62-year-old man presented with persistent vomitus. He had history of distal gastrectomy for gastric adenocarcinoma with H. pylori infection diagnosed one year prior. Post-surgery abdominal CT scan initially showed no residual mass. Current examination revealed evidence of gastric outlet obstruction due to recurrent mass formation. Endoscopic examination confirmed a mass obstructing the pylorus, with H. pylori infection. Due to the tumor's unresectable nature, the patient underwent triple bypass surgery. Omentum biopsy revealed metastatic adenocarcinoma likely originating from the stomach.</p><p><b><i>Discussion:</i></b> This case highlights the potential rise of H. pylori cases in regions with low prevalence. It emphasizes the necessity for additional research about risk factors associated with malignancy following H. pylori infection, especially in low prevalence regions.</p><p><b>PP-02-019</b></p><p><b>42 year old male clinically diagnosed with cowden syndrome: a case report</b></p><p><b>Pamela Bianca Yap</b>, Mara Mangio-Manansala and Frinz Moey Caunes Rubio</p><p><i>St.lukes medical center, Quezon city, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Cowden syndrome is part of PTEN hamartoma tumor syndromes (PHTS) and is characterized by autosomal dominant mutations of the PTEN tumor suppressor gene. The presence of hamartomas is the defining clinical feature. PHTS have increased risk of both benign and malignant tumors, including colon cancers. However, some patients with mutations do not show clinical signs and symptoms, while patients with the clinical syndrome may not have detectable PTEN mutations.</p><p><b><i>Case Description:</i></b> A 43-year-old man with macrocephaly and multiple trichilemmomas was diagnosed with benign nasopharyngeal mass, presenting as recurrent epistaxis. The patient was suspected to have PHTS hence referred for endoscopy to evaluate presence of gastrointestinal hamartomas. EGD revealed multiple esophageal glycogenic acanthosis. Colonoscopy showed innumerable polyps in the cecum, ascending and sigmoid colon. Biopsies of the polyps yielded juvenile polyps, tubular adenoma, and tubulovillous adenoma. No family member is suspected or confirmed to have PHTS. Based on the current National Comprehensive Cancer Network (NCCN) operational diagnosis of PHTS, patient satisfied three major criteria: macrocephaly, multiple trichilemmomas, and multiple juvenile polyps. Patient was then advised for colonoscopy every 2 years due to increased risk of developing colorectal cancer approximately 9 to 20%. Genetic counseling was advised for both the patient and family members.</p><p><b><i>Discussion:</i></b> PTEN gene mutations have poor penetrance. Even if PTHS is an autosomal dominant genetic condition, it may not be reliably detected in their family history. Screening colonoscopy is recommended because of increased risk of developing colorectal cancer. Moreover, annual comprehensive examinations are recommended for cancer surveillance.</p><p><b>PP-02-020</b></p><p><b>A Rare Case of Pancreatic Adenocarcinoma presenting as Colon mass: A Case Report</b></p><p><b>Pamela Bianca Yap</b> and Maria Angelica Franco</p><p><i>St.lukes medical center, Quezon city, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pancreatic adenocarcinoma is one of the most aggressive forms of malignancy. It commonly presents as abdominal pain and pancreatic duct obstruction. The lack of early symptomatology leads to delay in diagnosis with a 5-year survival rate of 8% in the advanced stage. It usually metastasizes in the liver, lymph nodes and peritoneum however, rarely metastasizes to the colon with only few reported cases. Patients have poor prognosis averaging 7 months after detection of colon metastasis.</p><p><b><i>Case Description:</i></b> Patient is a 52/M, heavy smoker presenting with crampy abdominal pain and 10kg weight loss 2 months prior to index admission. There was no reported changes in bowel movement or gastrointestinal bleeding. On physical examination, he is cachectic with diffuse abdominal tenderness. Colonoscopy was done showing a polypoid easily friable 2cm mass at the cecum. Biopsy showed adenocarcinoma with mucinous features which is not typical of primary colon cancer. CT scan of the whole abdomen with IV contrast revealed a gastric antral mass and peritoneal carcinomatosis. The pancreas was unremarkable. Further workup with upper endoscopy, upper EUS and exploratory laparotomy with peritoneal sampling all revealed metastatic carcinoma. Immunohistochemical(IHC) of the cecal mass showed metastatic carcinoma favoring a pancreatic vs. gastric origin. Subsequent additional IHC eventually revealed adenocarcinoma compatible with pancreaticobiliary origin.</p><p><b><i>Discussion:</i></b> Differentiating colon metastases from primary colon cancer can be challenging. When dealing with metastatic cancers, IHC can help identify the tissue of origin by detecting markers that are typical of specific primary tumors. This is crucial for guiding treatment decisions.</p><p><b>PP-02-021</b></p><p><b>“Hookworm-Induced Gastrointestinal Hemorrhage: A Case Study on Necator americanus\"</b></p><p><b>Meutia Yasrizal</b><sup>1</sup> and Muhammad Firhat Idrus<sup>1</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;</i> <sup>2</sup><i>Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hookworm infections in humans are typically caused by the helminth nematodes Necator americanus and Ancylostoma duodenale.1 Most infected individuals remain asymptomatic, with iron deficiency anemia resulting from chronic blood loss in the digestive tract 2</p><p><b><i>Case Description:</i></b> In this report , we discuss the case of a 64-year-old male patient presented with recurrent black stools for the past 6 months before hospital admission. The patient frequently visited the hospital due to weakness, and upon examination, his hemoglobin (Hb) levels were consistently low, requiring blood transfusions.</p><p>Laboratory results showed Hb 3.6 g/dL, hematocrit 13.8%, leukocytes 7210/uL, platelets 372,000/uL, MCV 77.5 fL, and MCH 20.2 pg. Differential count showed basophils at 0.1%, eosinophils at 11.3%, neutrophils at 67.9%, lymphocytes at 8.9%, and monocytes at 11.8%.</p><p>Complete feces examination revealed brown color, leukocytes 2-3 per high power Aield (HPF), erythrocytes 1-2 per HPF, and positive bacteria.</p><p>Colonoscopy results showed a normal colon. EGD results revealed a hiatal hernia and helminthiasis. Stool parasitology results showed hookworm eggs and adult Necator americanus.</p><p><b>PP-02-022</b></p><p><b>Phlebosclerotic colitis with chronic Traditional Chinese Medicine use: A case report</b></p><p><b>Pun Fung Yip</b>, Wing Chee Lai and Steven Woon Choy Tsang</p><p><i>Tseung Kwan O Hospital, Hong Kong</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Phlebosclerotic colitis (PC) is different from ordinary ischemic colitis with distinct radiological, endoscopic and histopathological features. Around 200 cases were reported until 2020, mainly affecting the Asian population. Possible etiologies include portal hypertension, right-sided heart failure, diabetes mellitus, vasculitis, hemodialysis and herbal use.</p><p><b><i>Case description:</i></b> A 59-year-old lady presented with abdominal pain and diarrhea. She took Traditional Chinese Medicine (TCM) for 10 years. CT showed venous calcification and sclerosis in ascending and transverse colonic mesentery, with colonic thickening and edema, suggestive of venous ischemic colitis. Colonoscopy showed dark-purple mucosa at ascending and transverse colon. Biopsies show hyalinization and fibrosis in lamina propria, with hyalinized and thickened walls at capillary and submucosal veins. Findings are suggestive of PC. Tumour markers, rheumatological and hematological workup are unremarkable. Gardenia jasminoides was identified in TCM, a possible cause of PC.</p><p>Patient improved with oral mesalazine and discontinued TCM. Follow-up CT at 1 year showed similar calcification at mesenteric veins.</p><p><b><i>Discussion:</i></b> A review analyzed herbal ingredients taken by 25 PC patients, all identified Geniposide. Geniposide is the major constituent of Gardenia fruits, commonly used in Kampo and TCM. Another study showed a positive correlation between the quantity of Gardenia-containing Chinese medical liquors consumption and the CT severity of PC.</p><p><b>PP-02-023</b></p><p><b>A successful endoscopic banding without resection for neuroendocrine tumor in duodenal bulb: a case report</b></p><p><b>Ahmad Fariz Malvi Zamzam Zein</b><sup>1,2</sup>, Hani Andriani<sup>3</sup>, Nunik Royyani<sup>4</sup>, Rabbinu Rangga Pribadi<sup>5</sup> and Bethy Suryawathy Hernowo<sup>6</sup></p><p><sup>1</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Swadaya Gunung Jati - Waled General Hospital, Cirebon, Indonesia;</i> <sup>3</sup><i>Department of Anatomical Pathology, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;</i> <sup>4</sup><i>Department of Radiology, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;</i> <sup>5</sup><i>Division of Gastroenterology, Pancreatoobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia;</i> <sup>6</sup><i>Department of Anatomical Pathology, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Duodenal neuroendocrine tumor (d-NET), a rare gastrointestinal tumor, remains challenge especially in limited settings. Safe and effective endoscopic management is preferable.</p><p><b><i>Case Description:</i></b> A 39-year-old male patient admitted to primary hospital with dyspepsia. Esophagogastroduodenoscopy identified duodenal bulb mass with central depression bulb (Fig 1a) and irregular microsurface and microvascular pattern on NBI (Fig 1b). Abdominal CT scan revealed 16-mm solitary duodenal mass with both arterial and venous phases enhancement and no lymph involvements or metastasis were detected. Histopathological findings revealed monotonous regular cells with round-and-oval nuclei and salt-and-pepper chromatin. Immunohistochemistry test showed positive for chromogranin and synaptophysin, negative for S-100, and Ki67 approximately <3%. Diagnosis of well-differentiated d-NET (G1) was confirmed.</p><p>EBWR was performed uneventfully without clinically active bleeding and pain post-EBWR. No adverse event was reported during 3-month evaluation visits.</p><p>Esophagogastroduodenoscopy at 3-month follow-up showed small wall-thickening at post-EBWR site with regular pattern on NBI view. Histopathological and immuhistochemistry finding confirmed negative for recurrence. Biennial surveillance esophagogastroduodenoscopy-biopsy was suggested in this case.</p><p><b><i>Discussion:</i></b> Histopathological features and positive for chromogranin and synaptophysin confirmed well-differentiated d-NET, while Ki-67 index <3% constituted low-grade (G1).</p><p>Endoscopic management is recommended in low-grade dNETs. Endoscopic resection is merely considered in advanced centers with carrying risk of complications. EBWR is preferred technique providing considerable effectiveness, feasible in any setting, almost nil complication, and inexpensive. This case reported safe and complete eradication of d-NET (G1) using EBWR.</p><p>Regarding the risk of recurrence, subsequent esophagogastroduodenoscopy-biopsy are recommended. Strategy for surveillance is enhanced.</p><p><b>PP-02-024</b></p><p><b>Rectal endometriosis biopsy targeted by magnification endoscopy narrow-band imaging (ME-NBI) and endoscopic ultrasonography (EUS)</b></p><p><b>Hongbin Zhu</b>, Ling Hu, suJuan Zhang and Yumeng Chen</p><p><i>Department of gastroenterology and hepatology, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Ectopic endometriosis is characterized by the presence of endometrial tissue outside the uterine cavity, leading to irritation, bleeding, and inflammation. In this case, we employed biopsy targeted by ME-NBI plus EUS and established the definitive diagnosis of rectal endometriosis.</p><p><b><i>Case description:</i></b> A 43-year-old female presented with intermittent abdominal pain and hematochezia. Colonoscopy revealed a broad-based, flat-elevated lesion in the sigmoid colon. The mucosal surface of the lesion exhibited uneven granularity, marked congestion, and edema (Figure A). ME-NBI examination revealed type II glandular openings, characterized by sparse microvascular density, accompanied by elongated and thickened blood vessels. Additionally, white opaque substances were observed within the glands (Figure B). EUS examination showed the disappeared stratification and significant thickeness of the wall. A buffalo horn-shaped, hypoechoic, and homogeneous pelvic mass measuring 24.5 × 24.0 mm was detected involving the posterior cervical wall and extending into the mucosal layer of the rectal wall (Figure C). A biopsy of the granular area revealed chronic inflammation with erosion in the rectal mucosa, as well as endometriosis in the lamina propria (Figure D). A gonadotropin-releasing hormone agonist (Leuprorelin) was administered via subcutaneous injection to induce atrophy of the endometriotic tissue and uterine fibroids. This treatment resulted in significant alleviation of the patient’s symptoms.</p><p><b>PP-02-025</b></p><p><b>A Case of lethal Mediastinitis after Intramural Esophageal Hematoma</b></p><p><b>Hongbin Zhu</b><sup>3</sup>, Ling Hu<sup>1</sup>, Jiguang Meng<sup>2</sup>, Jingwei Qiu<sup>1</sup> and Yanbing Liu<sup>1</sup></p><p><sup>1</sup><i>Department of respiratory medicine, the 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China;</i> <sup>2</sup><i>Department of Pulmonary and Critical Care Medicine, the fourth Medical Center of Chinese PLA General Hospital, Beijing, China;</i> <sup>3</sup><i>Department of gastroenterology and hepatology, the 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Intramural Esophageal Hematoma (IEH) arises from acute mucosal and submucosal injury, leading to blood accumulation in the esophageal wall. It is typically considered a benign disease with a generally favorable prognosis. Herein, we report a case of IEH complicated by subsequent letheal mediastinitis.</p><p><b><i>Case description:</i></b> A 53-year-old woman presented to our hospital with dysphagia for 10 days. The dysphagia onset accompanied by severe retrosternal pain and recurrent vomiting began following the ingestion of tablets 10 days prior. CT revealed diffuse thickening of the esophagus wall with fluid retention in the lumen and right-sided pleural effusion. Endoscopic examination identified a stenosis of the esophagus 25-35 cm from the incisors, along with a 1 cm-wide, strip-shaped, dark red elevation, bleeding easily (Figure A). Two days later, CT showed free air around the lower esophagus and below the diaphragm, with an increase in pleural effusion (Figure B). Thoracentesis yielded 250 ml of turbid, bloody pleural fluid. Her symptoms ameliorated following symptomatic treatment. However, two weeks later, the patient experienced episodic dyspnea, which progressively worsened, accompanied by neck swelling and coagulation dysfunction. CT confirmed a diagnosis of mediastinitis (Figure C). Regrettably, her condition progressively deteriorated and she died 35 days after initial presentation.</p><p><b><i>Discussion:</i></b> Based on the clinical manifestations, imaging and laboratory tests, we suspect the patient succumbed to pulmonary hypertension associated with fibrotic mediastinitis and subsequent right heart failure. This case underscores the importance of early diagnosis of IEH and, more significantly, elucidates the potential for IEH to progress to fatal mediastinitis.</p><p><b>PP-02-026</b></p><p><b>Early detection and management of acute superior mesenteric vein thrombosis following variceal hemostasis</b></p><p><b>Hongbin Zhu</b> and Ling Hu and Jing Li and Jiana Li and Lili Liu and Zhoupei Fan and Qinqin Xiao</p><p><i>Department of gastroenterology and hepatology, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China;</i> <i>Department of Respiratory Medicine, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Superior mesenteric venous thrombosis (SMVT) is a rare condition but portends a mortality rate of up to 23%. SMVT subsequent to endoscopic hemostasis for variceal bleeding in patients with cirrhosis are reported rarely. Here, we illustrate the management of such a rare condition.</p><p><b><i>Case Description:</i></b> All four patients presented with hematemesis and melena. Abdominal CT scans did not reveal any signs of mesenteric thrombosis. Approximately five days post-procedure, following endoscopic variceal ligation (EVL) and/or endoscopic injection sclerotherapy (EIS) for hemostasis, the patients reported different degrees of abdominal discomfort, bloating, and loss of appetite postprandially. Laboratory test indicated a significant increase in D-dimer levels, reaching a peak of 28 mg/L. The formation of SMVT was confirmed through enhanced abdominal CT scans or ultrasound. Following the subcutaneous administration of low molecular weight heparin (LMWH), the symptoms were markedly relieved and D-dimer levels decreased. Following their discharge, patients were administrated a daily oral dosage of 10 mg rivaroxaban. Regular clinical follow-ups were conducted combined with abdominal ultrasounds every four weeks. None of the patients exhibited any progression of thrombus. Notably, the thrombus had almost completely disappeared in one of those patients after 3 weeks.</p><p><b><i>Discussion:</i></b> EVL/EIS for variceal bleeding may potentially serve as a risk factor for acute SMVT. Thus, attention should be paid to the appearance of symptoms and elevation in the levels of D-dimer. The early commencement and sequential implementation of anticoagulation therapy appear to be both safe and effective. Abdominal ultrasound offers a cost-effective approach for monitoring SMVT.</p><p><b>PP-02-027</b></p><p><b>Efficacy and safety of endoscopic ultrasound-guided biliary drainage in managing malignant hilar biliary obstruction</b></p><p><b>Takuji Iwashita</b><sup>1</sup>, Shinya Uemura<sup>1</sup>, Akinori Maruta<sup>2</sup>, Keisuke Iwata<sup>3</sup> and Masahito Shimizu<sup>1</sup></p><p><sup>1</sup><i>Gifu University Hosopital, Gifu, Japan;</i> <sup>2</sup><i>Gifu Prefectural General Medical Center, Gifu, Japan;</i> <sup>3</sup><i>Gifu Municipal Hospital, Gifu, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>ERCP-guided biliary drainage (BD) has been employed as the primary intervention in managing malignant hilar biliary obstruction (MHBO). Recently, EUS-BD has emerged as a useful drainage technique; however, its efficacy in MHBO remains insufficiently explored.</p><p><b><i>Aims:</i></b> To assess the efficacy and safety of EUS-BD in MHBO.</p><p><b><i>Methods:</i></b> Patients who underwent EUS-BD for MHBO from 2012 to 2023 were retrospectively reviewed. The study outcomes were the technical success and clinical success rates, and safety.</p><p><b><i>Results:</i></b> 28 patients with MHBO were included with etiologies of bile duct cancer in 17, gallbladder cancer in 2, and metastatic lesions in 9. The obstruction types were Bismuth Type 2 in 9, Type 3A in 7, and Type 4 in 12. EUS-BD was performed as initial drainage in 10 and rescue drainage (s/p ERCP-BD) in 18. The technical success rate was 100% (28/28), with stent placement configuration of hepaticogastrostomy alone in 21, HGS with bridging (left to right system) in 3, antegrade stenting (AG: stenting at the obstruction) alone in 2, AG with bridging in 1, and AG with HGS in 1. The clinical success rate was 82% (23/28). Adverse events were observed in 3 (11%), all related to bile peritonitis. Comparisons of clinical failure with various clinical factors showed significant differences in Bismuth Type 4 and drainage area of less than 3 segments.</p><p><b><i>Conclusion:</i></b> EUS-BD is considered useful in the management of MHBO. However, careful consideration is required for its indication, as the clinical success rate was not exactly high. Further studies are warranted.</p><p><b>PP-02-028</b></p><p><b>The Influence of Intravenous Antispasmodics on Gastric Transit Time in Capsule Endoscopy</b></p><p><b>Dabin Kim</b></p><p><i>Gyeongsang national university hospital/Gastroenterology, Jinju-si, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> This study investigates the impact of intravenous (IV) antispasmodics on gastric transit time in capsule endoscopy and explores the influence of various risk factors on this transit time.</p><p><b><i>Methods:</i></b> Data from patients who underwent capsule endoscopy were analyzed. Patients were divided into two groups: those who received IV antispasmodics (Buscopan) on the same day as the procedure and those who did not receive IV antispasmodics. Additionally, we evaluated the impact of risk factors (alcohol, smoking, diabetes, hypertension) on gastric transit time. Statistical analysis, including t-tests, was performed to compare the mean transit times and assess the significance of differences.</p><p><b><i>Results:</i></b> A significant difference in gastric transit times was observed between the groups. Patients who received IV antispasmodics on the same day had a mean gastric transit time of 149.00 hours (SD = 200.94), while those who did not had a mean gastric transit time of 52.55 hours (SD = 63.49) (t = 2.543, p = 0.014). Risk factor analysis showed no statistically significant differences in gastric transit times for alcohol (p = 0.819), smoking (p = 0.854), diabetes (p = 0.873), and hypertension (p = 0.421).</p><p><b><i>Conclusion:</i></b> The use of IV antispasmodics is associated with prolonged gastric transit time in capsule endoscopy. Risk factors such as alcohol, smoking, diabetes, and hypertension do not significantly affect gastric transit time. These findings underscore the importance of considering pre-procedural medications when interpreting capsule endoscopy results and suggest that further research is needed to optimize clinical protocols.</p><p><b>PP-02-029</b></p><p><b>Urinary Lipoarabinomannan (LAM) -A simple test that aids in differentiating Intestinal Tuberculosis from Crohn's Disease</b></p><p>Mukesh Singh and <b>MANJEET Goyal</b> and Himanshu Narang and Malambo Mubbunu and Peeyush Kumar and Bhaskar Kante and Sudheer Vuyyuru and Srikant Mohta and Ashish Dutt Upadhyay and Prasenjit Das and Ankur Goyal and Raju Sharma and Govind Makharia and Saurabh Kedia and Vineet Ahuja</p><p><i>Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To evaluate the diagnostic accuracy of urinary LAM in distinguishing Intestinal Tuberculosis (ITB) from Crohn's Disease (CD) among patients presenting with clinical features of these conditions.</p><p><b><i>Methods:</i></b> A prospective study was conducted at a tertiary health care center from January 2021 to April 2022. The study included patients diagnosed with ITB, CD, or indeterminate conditions. Comprehensive evaluations, laboratory investigations, CT enterography, colonoscopy, and histopathological analyses were performed. Fresh morning midstream urine samples were collected and analyzed using the Alere Determine TB LAM Ag kit. The analytical team was blinded to clinical data. Statistical analysis determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall diagnostic accuracy of urinary LAM.</p><p><b><i>Results:</i></b> Among 98 patients, 36 were diagnosed with ITB and 62 with CD. Urinary LAM was positive in 9 out of 36 ITB patients, yielding a sensitivity of 25% (95% CI 12.12%-42.20%) and a PPV of 100% (95% CI 66.37%-100.00%). None of the CD patients tested positive for urinary LAM, resulting in a specificity of 100% (95% CI 94.22%-100.00%) and an NPV of 69.66% (95% CI 65.54%-73.50%). The overall diagnostic accuracy of urinary LAM in differentiating ITB from CD was 72.45%. Adding urinary LAM testing to existing diagnostic criteria improved accurate identification of ITB from 44% to 57%.</p><p><b><i>Conclusion:</i></b> Urinary LAM testing exhibits high specificity and PPV, making it a significant adjunct in the diagnostic process for ITB.</p><p><b>PP-02-030</b></p><p><b>Structured Yoga Practices significantly improve Quality of Life in Ulcerative Colitis: a randomized controlled trial</b></p><p><b>Janaki D B Sankar Kalum</b><sup>1</sup>, Himanshu Narang<sup>1</sup>, Akriti Shukla<sup>3</sup>, Govind K Makharia<sup>1</sup>, Saurabh Kedia<sup>1</sup>, Peeyush Kumar<sup>1</sup>, David Mathew Thomas<sup>1</sup>, Shubham Prasad<sup>1</sup>, Malamboo Mubbunu<sup>1</sup>, Shubhi Virmani<sup>1</sup>, Divya Madan<sup>1</sup>, Mahak Verma<sup>1</sup>, Gautam Sharma<sup>2</sup> and Vineet Ahuja<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi NEW DELHI, India;</i> <sup>2</sup><i>Department of Cardiology and Professor In-charge, Center for Integrative Medicine and Research (CIMR), All India Institute of Medical Sciences, New Delhi NEW DELHI, India;</i> <sup>3</sup><i>Centre for Integrative Medicine and research, AIIMS, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Objective:</i></b> There are anecdotal studies on effect of Yoga in patients with ulcerative colitis (UC). We investigated the effect of yoga on health-related quality of life and disease activity in UC patients in remission.</p><p><b><i>Materials and Methods:</i></b> Patients with UC in remission (SCCAI <3 and fecal calprotectin<150 μg/mg) were randomly assigned to either IBD-specific yoga therapy (45 minutes/day, five days/week) or brisk walking (150 minutes/week), in addition to standard medical treatment, for 6 months. Primary outcome was the effect of yoga on quality of life using the World Health Organization Quality of Life – BREF (WHOQOL-BREF), Hamilton Anxiety Rating Scale (HAM-A), and Perceived Stress Scale (PSS) questionnaires at 6 months. Secondary outcome was relapse rates at 6 months.</p><p><b><i>Results:</i></b> 100 patients were randomized with 50 in each arm and 5 were lost to follow up. Baseline clinical and demographic characteristics and WHOQOL BREF, PSS and HAM-A scores were similar. At 6 months, patients in yoga group showed significantly lower PSS (16.70±1.44 vs. 17.34±1.5, p=0.03) and anxiety scores (1.51±0.68 vs. 1.89±0.79, p=0.01) compared to walking. Yoga also significantly improved physical (71.29±2.94 vs. 69.65±3.4, p=0.014) and psychological well-being (71.55±4.28 vs. 68.34±4.77, p=0.001), with similar social scores (63.85±6.98 vs 63.47±6.68 p=0.79). One patient in each group had disease relapse.</p><p><b><i>Conclusion:</i></b> Yoga can be considered an effective ancillary intervention to improve the quality of life in patients with UC in remission.</p><p><b>PP-02-031</b></p><p><b>Medication Non-Adherence in Inflammatory Bowel Disease Patients in a resource-constrained setting</b></p><p><b>Md Yasir Arafat</b>, M Masudur Rahman, Rashedul Hasan and Md Iqbal Hossain</p><p><i>Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Since drug adherence is an important issue in maintaining remission in inflammatory bowel disease (IBD) in a resource-constrained country like Bangladesh, we planned to conduct a study to determine the drug non-adherence rate, predicting factors, and the reason associated with non-adherence.</p><p><b><i>Materials and Methods:</i></b> This prospective cross-sectional study was done at Sheikh Russel National Gastroliver Institute and Hospital, Bangladesh. The frequency of missed doses was evaluated based on recall of drug adherence from the previous two weeks. Medication intake of <80% of the advised dose was defined as non-adherence.</p><p><b><i>Results:</i></b> A total of 272 patients [94(34.6%) Ulcerative colitis (UC) and 178(65.4%) Crohn’s disease (CD)] were included. The mean age was 32.1±10.6 years, Male: 174(64%), median disease duration: 2.0±2.07 years. The overall rate of non-adherence was 46% [38/94(40.4%) UC and 87/178(48.9%) CD]. Univariate analysis identified urban residency and longer disease duration as predicting factors associated with non-adherence. Age, sex, marital status, education and IBD types were not significant predictors (Table 1). Similarly, multivariate analysis revealed longer disease duration (AOR 1.14, CI 1.00-1.30, P=0.04) and urban residency (AOR 0.54, CI 0.31-0.94, P=0.03) as significant predictors of non-adherence. The main reasons for non-adherence were forgetfulness 43(34.4%), unavailability of drugs 35(28%), felt better 28(22.4%), lack of adequate information 27(21.6%), cost of drugs 21(16.8%).</p><p><b><i>Conclusion:</i></b> Almost half of the patients were non-adherent to their IBD medication. The key predictors of non-adherence were longer disease duration and urban residency. The most common reasons for non-adherence were forgetfulness and the unavailability of drugs.</p><p><b>PP-02-032</b></p><p><b>Knowledge, Attitudes and Practice about Vaccination in Inflammatory Bowel Diseases</b></p><p><b>Md Yasir Arafat</b>, M Masudur Rahman, Azam Jahangir, Karzon Dey Sarker, Shahana Parvin, Md Kamrul Hasan and Md Musab Khalil</p><p><i>Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Although guidelines suggest vaccination against various infections in inflammatory bowel disease (IBD), the practice varies in different populations. Hence, we aimed to study vaccination rates and knowledge of and attitudes toward vaccination among a cohort of Bangladeshi IBD patients.</p><p><b><i>Materials and Methods:</i></b> Consecutive IBD patients attending the IBD clinic at Sheikh Russel National Gastroliver Institute and Hospital, Bangladesh were interviewed with a standardised validated questionnaire on self-reported vaccination.</p><p><b><i>Results:</i></b> A Total of 224 patients with IBD were included [male, 139 (62.1%); median age 32 years (interquartile range, 24.3–41)]. Of all, 78/224(34.8%) were ulcerative colitis (UC) and 146/224(65.2%) crohn’s disease (CD). Self-reported vaccine uptake was for hepatitis B virus 83/224(36.6%) [UC(22/78, 28.2%) CD(61/146, 41.8%),p=0.06]; seasonal influenza 78/224 (34.8%) [UC(18/78,23.1%) CD (60/146, 41.1%),p<0.01]; pneumococcus 76/224 (33.9%) [UC 17/78,21.8%), CD (59/146,40.4%),p<0.01]; measles, mumps and rubella (MMR) 183/224(81.7%) [UC(71/78,91%), CD(112/146,76,7%),p=0.01]; diphtheria, pertussis and tetanus (DPT) 183/224 (81.7%) [UC(70/78,89.7%, CD(113/146,77.4%),p=0.03]; Varicella 0/224(0%); Human papilloma vaccine (HPV) 5/85 (5.9%).</p><p>Overall, only 26.8% were vaccinated against three or more adult vaccines. Use of steroid (AOR [adjusted odds ratio] 15.6, 95% confidence interval [CI] 6.3–38.5, P<0.001), immunosuppressive (AOR 6.1, 95% CI 2.5– 15, P<0.001), and biological agents (AOR 20, 95% CI 2.8–139.8, P=0.002) were associated with at least three or more adult vaccines. The major reasons for non-vaccination were not knowing the presence of the vaccine 116/224(51.8%), necessity of the vaccine 117/224(52.2%) and non-affordability 50/224(22.3%)(Table 1).</p><p><b><i>Conclusion:</i></b> Adult vaccination rates were suboptimal for patients with IBD. Lack of Knowledge and awareness are the main reasons for non-vaccination.</p><p><b>PP-02-033</b></p><p><b>Incidence, risk factors, and outcomes of chronic antibiotic-refractory pouchitis in Korean patients with ulcerative colitis</b></p><p><b>Ji Eun Baek</b><sup>1,3</sup>, Jung-Bin Park<sup>1</sup>, June Hwa Bae<sup>1</sup>, Min Hyun Kim<sup>2</sup>, Seung Wook Hong<sup>1</sup>, Sung Wook Hwang<sup>1</sup>, Jong Lyul Lee<sup>2</sup>, Yong Sik Yoon<sup>2</sup>, Dong-Hoon Yang<sup>1</sup>, Byong Duk Ye<sup>1</sup>, Jeong-Sik Byeon<sup>1</sup>, Seung-Jae Myung<sup>1</sup>, Chang Sik Yu<sup>2</sup>, Suk-Kyun Yang<sup>1</sup> and Sang Hyoung Park<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea;</i> <sup>2</sup><i>Division of Colon and Rectal Surgery, Department of Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea;</i> <sup>3</sup><i>Department of Gastroenterology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> The study investigated the incidence, risk factors, and clinical outcomes of chronic antibiotic-refractory pouchitis (CARP) in Korean patients with ulcerative colitis (UC).</p><p><b><i>Methods:</i></b> This single-center retrospective study included patients with UC who underwent total proctocolectomy with ileal pouch-anal anastomosis (IPAA) at the Asan Medical Center in Korea between January 1987 and December 2022. Primary outcomes were endoscopic remission and pouch failure. Cox’s proportional hazard model was used to identify risk factors of CARP.</p><p><b><i>Results:</i></b> A total of 232 patients were analyzed. The most common cause of surgery was steroid refractoriness (50.9%), followed by dysplasia/colorectal cancer (26.7%). Among 74 patients (31.9%) with chronic pouchitis (CP), 31 (13.4%) had CARP, and 43 (18.5%) had chronic antibiotic-dependent pouchitis (CADP). The most frequent endoscopic phenotype was focal inflammation of the pouch (CP, 47.3%; CARP, 35.5%; CADP, 55.8%). Patients with CARP were less likely to have concomitant probiotics compared with CADP (29.0% vs 72.1%; p<0.01). The endoscopic remission rate in CP, CARP, and CADP was 14.9%, 9.7%, and 18.6%, respectively. The pouch failure rate in CP, CARP, and CADP was 13.5%, 16.1%, and 11.6%, respectively. Current smoking status (adjusted Hazard Ratio [aHR] 2.96; 95% confidence interval [CI] 1.27–6.90; p=0.01) and previous use of biologics/small molecules (aHR 2.40; 95% CI 1.05–5.53; p=0.04) were significantly associated with CARP development.</p><p><b><i>Conclusions:</i></b> Current smoker and previous user of biologics/small molecules in UC patients who underwent IPAA had a higher risk of CARP. Concomitant use of probiotics was less likely to be associated with developing CARP.</p><p><b>PP-02-034</b></p><p><b>Efficacy and Safety of Vedolizumab-Tofacitinib(VETO) combination in Ulcerative-Colitis refractory to Anti-TNF and second-line advanced therapy</b></p><p><b>Rupa Banerjee</b>, Pardhu Bharath Neelam, Dhanush Mekala, Nalini Raghunathan and Rajendra Patel</p><p><i>AIG Hospitals, Hyderabad, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Management of refractory Ulcerative colitis (UC) not responding to multiple advanced therapies is challenging. Role of combination therapy in management of refractory UC is evolving. We studied safety and efficacy of Vedolizumab and Tofacitinib combination(VETO) therapy in anti-TNF refractory patients unresponsive to either Vedolizumab or Tofacitinib alone.</p><p><b><i>Materials and methods:</i></b> We prospectively recruited consecutive UC patients who were anti-TNF non-responsive after consent. They were randomised to either Tofacitinib or Vedolizumab. Non-responders with second-line agents were given VETO therapy and followed for 24 weeks(Figure1). Clinical response (week 8), clinical and endoscopic remission rates (week 24) and need for colectomy were assessed. All adverse events were recorded. Paired t-test was used to compare the mean Mayo scores at the baseline and end of the follow-up.</p><p><b><i>Results:</i></b> Of 91 anti-TNF refractory patients, Vedolizumab and Tofacitinib were given as second-line therapy to 38 and 40 respectively. Twenty-four non-responders and partial responders (mean40.8±11.7yrs, females 63%) received VETO therapy. At week 8, 5(20.8%) and 18(75%) achieved clinical remission and response respectively. At week 24, 17(70.8%), 14(58.3%), 7(33.3%) achieved clinical response, remission and endoscopic remission respectively. Four(16.6%) required steroid rescue therapy. Three(12.5%) required colectomy by the end of 8 weeks(Figure1). Compared to baseline, Mean difference in Mayo score at 24-weeks is 5.33±0.53(p<0.01). Two developed pseudomembranous colitis which responded to vancomycin. No severe adverse events were noted.</p><p><b><i>Conclusion:</i></b> The VETO combination appears to be an effective and safe option for the management of refractory UC with more than 50% achieving clinical remission at 24weeks.</p><p><b>PP-02-036</b></p><p><b>Plant-based diets mitigate the incidence risk, disease course and comorbidity of inflammatory bowel disease</b></p><p><b>Jie Chen</b><sup>1</sup>, Yuhao Sun<sup>1,3</sup>, Lintao Dan<sup>1,2</sup>, Judith Wellens<sup>4,5</sup>, Shuai Yuan<sup>6</sup>, Hong Yang<sup>7</sup>, Tammy Tong<sup>8</sup>, Amanda Cross<sup>9</sup>, Nikolaos Papadimitriou<sup>10</sup>, Antoine Meyer<sup>11</sup>, Christina Dahm<sup>12</sup>, Susanna Larsson<sup>6,13</sup>, Alicja Wolk<sup>6</sup>, Jonas Ludvigsson<sup>14,15</sup>, Kostas Tsilidis<sup>16,17</sup>, Edward Giovannucci<sup>18,19</sup>, Jack Satsangi<sup>5</sup>, Xiaoyan Wang<sup>1</sup>, Evropi Theodoratou<sup>20,21</sup>, Simon Chan<sup>22,23</sup> and Xue Li<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, China;</i> <sup>2</sup><i>Department of Big Data in Health Science School of Public Health and The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;</i> <sup>3</sup><i>Department of Gastroenterology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;</i> <sup>4</sup><i>Department of Chronic Diseases and Metabolism, Translational Research Centre for Gastrointestinal Disorders (TARGID), KU Leuven, Leuven, Belgium;</i> <sup>5</sup><i>Translational Gastroenterology Unit, Nuffield Department of Medicine, Experimental Medicine Division, University of Oxford, John Radcliffe Hospital, Oxford, UK;</i> <sup>6</sup><i>Unit of Cardiovascular and Nutritional Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden;</i> <sup>7</sup><i>Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China;</i> <sup>8</sup><i>Cancer Epidemiology Unit, Nuffield Department of Population Health, University of Oxford, Richard Doll Building, Old Road Campus, Oxford, UK;</i> <sup>9</sup><i>School of Public Health and Department of Surgery and Cancer, Imperial College London, London, UK;</i> <sup>10</sup><i>Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France;</i> <sup>11</sup><i>INSERM, Centre for Research in Epidemiology and Population Health, Institut Gustave Roussy, Université Paris Saclay, Villejuif, France;</i> <sup>12</sup><i>Department of Public Health, Aarhus University, Bartholins Allé 28000, Aarhus, Denmark;</i> <sup>13</sup><i>Department of Surgical Sciences, Uppsala University, Uppsala, Sweden;</i> <sup>14</sup><i>Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden;</i> <sup>15</sup><i>Department of Pediatrics, Orebro University Hospital, Orebro, Sweden;</i> <sup>16</sup><i>Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London, UK;</i> <sup>17</sup><i>Department of Hygiene and Epidemiology, University of Ioannina School of Medicine, Ioannina, Greece;</i> <sup>18</sup><i>Department of Epidemiology, Harvard T.H. Chan School of Public Health, Massachusetts, USA;</i> <sup>19</sup><i>Department of Nutrition, Harvard T.H. Chan School of Public Health, Massachusetts, USA;</i> <sup>20</sup><i>Centre for Global Health Research, Usher Institute, University of Edinburgh, Edinburgh, UK;</i> <sup>21</sup><i>Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK;</i> <sup>22</sup><i>Department of Gastroenterology, Norfolk and Norwich University Hospital NHS Trust, Norwich, UK;</i> <sup>23</sup><i>Department of Medicine, Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich, UK</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the relationship between plant-based diets and the incidence and course of inflammatory bowel disease (IBD) across two large prospective cohorts.</p><p><b><i>Materials and Methods:</i></b> Data from the UKB including 187,888 participants free of IBD and 2,133 IBD patients and the EPIC cohort including 341,539 individuals free of IBD. The plant-based diet indexes (healthy and unhealthy PDI) were constructed based on the 24-h dietary recalls and food frequency questionnaires. Cox regression was applied to estimate hazard ratios (HRs), and interactions between genetics and plant-based diets were explored.</p><p><b><i>Results:</i></b> Over a median follow-up of 11.6 years in the UKB, 925 new IBD cases were recorded. Higher adherence to a healthy PDI was linked to a lower IBD risk (HR 0.75, 95% CI 0.60-0.94), while an unhealthy PDI was associated with increased risk (HR 1.48, 95% CI 1.21-1.82). Among individuals with established IBD, we observed significant associations of healthy PDI (HR 0.50, 95% CI 0.30 to 0.83) and unhealthy PDI (HR 2.12, 95% CI 1.30 to 3.44) with IBD-related surgery. In the EPIC cohort, similar estimation and trends were observed with 548 incident cases over a median follow-up of 14.5 years, with HRs of 0.71 (95% CI 0.59-0.85) for healthy and 1.54 (95% CI 1.30-1.84) for unhealthy PDI. The diet-IBD association was partially mediated by inflammation and more pronounced in genetically susceptible individuals.</p><p><b><i>Conclusion:</i></b> Adherence to a healthy plant-based diet may reduce the risk and improve outcomes for IBD, especially in those with higher genetic risk.</p><p><b>PP-02-037</b></p><p><b>Identification of highly-virulent strain from inflammatory bowel disease and characterizing the pathogenicity at transcriptome level</b></p><p><b>Jingyi Chen</b>, Congyi Yang, Yalan Xu, Yuzhen Zhao, Jushan Wu, Yulan Liu and Ning Chen</p><p><i>Peking University People's Hospital, Beijing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We characterize the pathogenicity differences of potential pathogens isolated from the saliva of inflammatory bowel disease (IBD) patients at the transcriptome level.</p><p><b><i>Materials And Methods:</i></b> Streptococcus mutans (S.m) strains were isolated from the saliva of IBD patients with different disease phenotypes. Differential gene expression, module clusters, protein-protein interaction, function annotation and enrichment was used to characterize the pathogenicity of S.m at the transcriptome level.</p><p><b><i>Results:</i></b> PCA analysis showed that there were differences between pathogenic and non-pathogenic strains at the transcriptome level. Pathogenic strains had 240 up-regulated and 178 down-regulated genes compared to non-pathogenic strains. We identified 395 virulence genes in S.m isolates and obtained 80 overlapped genes after comparing virulence genes with differential genes. The protein-protein interaction (PPI) network was constructed and identify the top 10 hub genes with the highest connectivity. The module consisting of the most closely interacting genes were identified and 5 key node genes (SMU_833, gtfD, luxS, spaP, rgpE) were obtained after comparing hub genes with module genes. In addition, differential genes of pathogenic/non-pathogenic isolates were enriched in functional pathways which is closely related to carbohydrate metabolism. Therefore, 45 carbohydrate active enzyme-related genes were identified and sacB, rgpE, SMU_833 were identified as key nodes in the PPI network.</p><p><b><i>Conclusion:</i></b> This study is the first to find that S. m isolates from UC patients have different pro-inflammatory effects. Identifying virulence genes of potential oral pathogens at the transcriptome level is helpful to reveal the mechanisms of different disease development pattern in IBD.</p><p><b>PP-02-038</b></p><p><b>Ustekinumab for the management of Crohn’s disease: Experience from Middle-East</b></p><p><b>Thomas Cherukara</b>, Noble Thomas, Yousif Al Serkal and Amit Kumar Dutta</p><p><i>Sharjah, Emirates Health Services, Kuwaiti Hospital, Sharjah, United Arab Emirates</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Ustekinumab is approved for therapy of moderate to severe Crohn’s disease. We report the efficacy and safety of this drug in management of a mixed group (prior biological therapy and treatment naïve) of patients with Crohn’s disease.</p><p><b><i>Methodology:</i></b> Patients with a diagnosis of Crohn’s disease and treated with Ustekinumab from 2019 to 2023 were retrospectively recruited. The clinical, demographic and investigation details were recorded on a structured data collection form. The details of therapy with Ustekinumab, response to therapy and therapy prior to Ustekinumab use were noted. The primary aim was the proportion of patients achieving clinical and biochemical remission (normalization of CRP or fecal calprotectin) after starting treatment.</p><p><b><i>Results:</i></b> Eighteen patients were included in this study. Table 1 shows the clinical features of the study subjects. Five patients had received prior biological therapy. The median fecal calprotectin value was 1000 microgram/gram and mean CRP value was 37+31mg/L before treatment. The median duration of follow-up was 15.5 months (range 3-44 months). Nine out of the eighteen patients achieved the primary end point after a median period of 6 months (range 3-12). Three patients improved clinically but did not achieve the primary end point. In two non-responders, the frequency of therapy was increased to every 4 weeks but was not effective. Serious adverse effect was noted in one patient who developed infected abdominal collection.</p><p><b><i>Conclusions:</i></b> Ustekinumab therapy in Crohn’s disease (prior biological therapy and treatment naïve) is generally safe and leads to clinical and biochemical remission in 50% of patients.</p><p><b>PP-02-039</b></p><p><b>Enhancing the predictions of cytomegalovirus infection in severe ulcerative colitis using deep learning ensemble model</b></p><p><b>A Reum Choe</b><sup>1</sup>, Jeong Heon Kim<sup>2</sup>, Ju Ran Byun<sup>1</sup>, Yehyun Park<sup>1</sup>, Eun Mi Song<sup>1</sup>, So Hyun Ahn<sup>3</sup> and Sung Ae Jung<sup>1</sup></p><p><sup>1</sup><i>Ewha Medical University Hospital, Seoul, South Korea;</i> <sup>2</sup><i>Department of Medicine, Yonsei University College of Medicine, South Korea;</i> <sup>3</sup><i>Ewha Medical Artificial Intelligence Research Institute, Ewha Womans University College of Medicine, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Cytomegalovirus (CMV) reactivation is common among patients with severe ulcerative colitis (UC), resulting in poorer prognoses than patients without CMV reactivation. The principal diagnostic approach for CMV involves biopsies, which are time-consuming and present challenges for early detection. To tackle this issue, our study utilizes deep learning to differentiate CMV from severe UC using endoscopic imaging, thereby enabling early CMV diagnosis.</p><p><b><i>Materials and Methods:</i></b> In this study, we examined 86 endoscopic images employing an ensemble of deep learning models, notably Densenet 121 pre-trained on ImageNet, to discriminate between cases of UC with and without CMV complications. Extensive preprocessing and test-time augmentation (TTA) techniques were applied to enhance the effectiveness of the models. Evaluation of the models' performance included metrics such as accuracy, precision, recall, F1 score, receiver operating characteristic (ROC) curves, and AUC values, highlighting the potential of deep learning to improve non-invasive gastroenterology diagnostics.</p><p><b><i>Results:</i></b> An ensemble of four models augmented with TTA demonstrated superior performance in classifying UC endoscopic images. It attained an accuracy of 0.83, precision of 0.83, recall of 0.91, and an F1-score of 0.87. These metrics underscore the ensemble's reliability and well-rounded performance. Particularly noteworthy is the substantial decline in performance metrics observed in models without TTA, highlighting the critical role of TTA.</p><p><b>PP-02-040</b></p><p><b>Appendectomy Alleviates Colonic Inflammation via Secondary Bile Acid-Induced Regulation of Lipid Metabolic Pathways</b></p><p><b>Feifei Chu</b><sup>1,2</sup>, Jie Xiong<sup>1</sup> and Hailong Cao<sup>1</sup></p><p><sup>1</sup><i>Tianjin Medical University General Hospital, Tianjin, China;</i> <sup>2</sup><i>Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The specific role of the appendix as both an immune organ and a reservoir for gut microbiota in inflammatory bowel disease(IBD) remains elusive. Recent clinical studies have demonstrated that appendectomy can alleviate ulcerative colitis(UC). However, the underlying mechanisms are still unclear. This study aims to comprehensively explore the precise regulatory mechanisms of appendectomy in the process of colonic inflammation.</p><p><b><i>Materials and Methods:</i></b> Appendectomy was performed on C57BL/6 mice followed by induction of dextran sulfate sodium (DSS)-induced colitis. Changes in colon length, body weight, and DAI score were evaluated, qPCR, western blot, and immunofluorescence staining were employed to assess production of pro-inflammatory cytokines and the level of colonic mucosal barrier markers. Public databases were utilized for bioinformatics analysis. Transcriptomic, 16S rRNA, and metabolomic sequencing were conducted.</p><p><b><i>Results and Conclusion:</i></b> Appendectomy increased colon length, body weight, and enhanced mucosal barrier integrity in mice with colitis, indicating alleviation of colitis. 16S rRNA sequencing indicated significant differences in microbial taxa such as Mucispirillum, Bacleroides, Clostridium_sensu_stricto_1 and Turcicibacter genus. Metabolomics analysis suggested that secondary bile acids accumulation. Both tanscriptome sequencing and metabolomics analysis enriched in lipid metabolism.</p><p><b>PP-02-041</b></p><p><b>Association of Psychological Distress and Resilience with Inflammatory Bowel Disease Activity and Quality of Life</b></p><p><b>Jaeyoung Chun</b> and Yuna Kim and Jie-Hyun Kim and Young Hoon Youn and Hyojin Park</p><p><i>Yonsei University College Of Medicine, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We evaluated the association of psychological distress and resilience with HRQoL and disease activity in patients with IBD.</p><p><b><i>Materials and Methods:</i></b> A total of 225 consecutive patients with Crohn’s disease (CD, n=108) and ulcerative colitis (UC, n=117) were enrolled at Gangnam Severance Hospital in Korea from 2021 to 2024. All participants completed self-reported questionnaires, including hospital anxiety and depression scale, perceived stress scale, the Korean resilience questionnaire, and the short IBD questionnaire. Disease activity was assessed using the Crohn's Disease Activity Index (CDAI) and the Harvey-Bradshaw index for CD, and the partial Mayo score and Simple Clinical Colitis Activity Index for UC, respectively.</p><p><b><i>Results:</i></b> HRQoL was significantly poorer in active IBD compared to quiescent IBD. Poor HRQoL was significantly associated with anxiety (p<0.001), depression (p=0.018), and stress (p=0.002), but not resilience, in a dose-response relationship. Significant association of anxiety (p=0.010), depression (p<0.001), stress (p=0.005) with disease activity was detected in patients with CD, but not UC. Regardless of inflammatory biomarkers, disease activity was significantly associated with anxiety (p=0.050), depression (p<0.001) and stress (p=0.019) in patients with CD. In terms of CDAI components, subscores for general well-being were associated with psychological distress whereas stool frequency and abdominal pain were not.</p><p><b><i>Conclusion:</i></b> HRQoL was significantly associated with psychological distress, but not resilience in patients with IBD. Psychological distress was associated with disease-related symptoms in patients with CD, particularly affecting general well-being. Physicians should be aware of the impact of psychological distress on patient-reported outcomes and HRQoL in the management of IBD.</p><p><b>PP-02-042</b></p><p><b>Muscle mass is preserved with dietary interventions in Crohn’s disease when using bedside muscle ultrasound</b></p><p><b>Jessica Fitzpatrick</b><sup>1</sup>, Peter R Gibson<sup>1</sup>, Daniel Clayton-Chubb<sup>1,2</sup>, Kirstin M Taylor<sup>2</sup> and Emma P Halmos<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, School of Translational Medicine, Monash University, Melbourne, Australia;</i> <sup>2</sup><i>Department of Gastroenterology, Alfred Health, Melbourne, Australia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To measure and compare the performance of point-of-care techniques in measuring body composition in Crohn’s disease patients and evaluate the subsequent impact of two high-quality dietary interventions differing in emulsifier content but iso-caloric to habitual diet.</p><p><b><i>Material and Methods:</i></b> In a double-blinded randomised controlled-feeding trial, adults with active Crohn’s disease received 4 weeks of either diet. Body composition assessments were completed at days 0 and 28, including dual energy X-ray absorptiometry (DXA, day 0 only), quantitative muscle ultrasound (QMUS), waist circumference, visceral fat via bioimpedance analysis (BIA) and hand-grip strength.</p><p><b><i>Results:</i></b> In 24 participants (mean age 37 (95% CI 32-41) years, 12 male), myopenia was present on DXA at baseline in 50% women and 9% men, but all had normal BMI and none had abnormal hand-grip strength. 25% women and 36% men were osteopenic. Central adiposity was present in 50%. QMUS correlated well with DXA appendicular skeletal muscle mass (ASMI) (r=0.92; p<0.001), and QMUS-predicted ASMI demonstrated no bias and narrow limits of agreement (LOA) on Bland-Altman analysis (Figure). The mean difference of BIA ASMI vs DEXA ASMI was 7.15kg (LOA 2.3-12.0).The diets consistently reduced weight by 2.2 [1.3,3.2] kg (p=0.009), waist circumference by 4% (p<0.001) and visceral adipose tissue by 16% (p<0.001) without a change of QMUS (p=0.78).</p><p><b>PP-02-043</b></p><p><b>Effect of tofatinib and upadacitinib in the treatment of anti-TNF refractory ulcerative colitis</b></p><p><b>Maiko Ikenouchi</b> and Hirokazu Fukui and Soichi Yagi and Koji Kaku and Yoko Yokoyama and Koji Kamikozuru and Mikio Kawai and Toshiyuki Sato and Norio Nishii and Kumiko Nakamura and Hirotsugu Eda and Yoshitaka Kitayama and Hiroo Sei and Takuya Okugawa and Toshihiko Tomita and Shinichiro Shinzaki</p><p><i>Hyogo Medical University, Nishinomiya, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Janus kinase (JAK) inhibitors including tofatinib (TOF) and upadacitinib (UPA) are frequently applied for patients with anti-TNF refractory ulcerative colitis (UC); however, their effects for those patients are remained unclear. Therefore, we investigated the effect of TOF and UPA on the clinical remission in patients with anti-TNF refractory UC.</p><p><b><i>Methods:</i></b> The patients who had undergone anti-TNF therapy and thereafter received either TOF or UPA were enrolled. Primary outcomes were efficacy at 8 weeks after administration of TOF or UPA to those patients. Clinical remission was defined as partial Mayo Score (PMS) ≤2 and each subscore ≤1. Clinical response was defined as follows: the decrease of PMS from baseline is ≥2 and rectal bleeding subscore is ≤1 or its decrease from baseline is ≥1. Primary anti-TNF refractory was defined as failure to clinical remission by anti-TNF.</p><p><b><i>Results:</i></b> Among 230 UC patients investigated, 170 received TOF and 60 received UPA treatment. The clinical response rate at 8 weeks after UPA treatment (81.7%) was higher than that after TOF treatment (66.5%). The clinical remission rate in UPA group (70.0%) was significantly greater than that in TOF group (44.7%) (OR, 2.87; 95%CI, 1.47-5.76). The clinical response rate in patients with primary anti-TNF refractory was 56.7% and 77.0% in TOF and UPA group, respectively. The clinical remission rate in those patients was significantly higher in UPA (77.0%) than in TOF (32.3%) group (OR, 3.3; 95%CI, 1.18-9.70).</p><p><b><i>Conclusions:</i></b> UPA is may be a useful JAK inhibitor for the therapy of anti-TNF refractory UC.</p><p><b>PP-02-044</b></p><p><b>Safety and Efficacy of Allopurinol to Optimize Azathioprine Therapy in Inflammatory Bowel Disease: A Meta-Analysis</b></p><p><b>Ni Kadek Saras Dwi Guna</b></p><p><i>Faculty of Medicine, Udayana University, Bali, Badung, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Although thiopurines are used as conventional therapy in maintaining moderate-severe IBD remission, its hepatotoxicity due to high 6-MMP production and lack of efficacy remain unsolved. Recent studies have combined thiopurines with allopurinol to reduce hepatotoxicity and improve efficacy. This study aims to evaluate efficacy and toxicity after this combination therapy.</p><p><b><i>Materials and Methods:</i></b> Systematic searches on Pubmed, Science Direct, and Google Scholar resulted in studies comparing IBD severity, hepatotoxicity, and bone marrow suppression before (thiopurine monotherapy) and after combination therapy. Review studies were excluded. The primary outcomes were 6-MMP, 6-TGN, and 6-MMP/6-TGN ratio. Secondary outcomes included ALT, AST, HBI Score, Partial Mayo Score, and WBC levels</p><p><b><i>Results:</i></b> This study included 11 studies with total of 294 subjects that met the inclusion criteria. The pooled result showed that ALT and AST levels were significantly reduced after combination therapy (MD=-43.53; 95% CI=-62.06 to -25.00; p=0.00 and MD=-11.72; 95% CI=-17.11 to -6.33; p=0.00, respectively), supported by significantly lower 6-MMP level (MD=-8816.96; 95% CI=-10.004 to -7548.44; p=0.00). HBI and Partial Mayo scores were also lower after combination therapy (MD=-3.57; 95% CI=-4.05 to -3.08; p=0.00 and MD=-0.86; 95% CI=-1.44 to -0.28; p=0.00, respectively). However, the WBC level was significantly reduced after combination therapy (MD=-1.35; 95% CI=-2.47 to -0.23; p=0.00), due to significantly higher 6-TGN level (MD=136.25; 95% CI=102.63 to 169.88; p=0.00). The 6-MMP/6-TGN ratio was significantly decreased after combination therapy (MD=-67.61; 95% CI=-82.90 to -52.32; p=0.01).</p><p><b><i>Conclusion:</i></b> Allopurinol-Thiopurine yielded significant hepatotoxicity and IBD severity reductions, yet associated with increased bone marrow suppression.</p><p><b>PP-02-045</b></p><p><b>Clinical Features of Ulcerative Colitis with Hypozincemia and Considerations for Zinc Replacement Therapy</b></p><p><b>Akira Hashimoto</b>, Syoma Sawai, Takahiro Ono, Marie Kurebayashi, Yuichi Tahara, Naoki Kuroda, Masatoshi Aoki, Hiroyuki Fuke, Hiroyuki Kawabata, Yoshihiro Wakita and Atsuya Shimizu</p><p><i>Saiseikai Matsusaka General Hospital, Matsusaka city, Mie Prefecture, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives</i></b>: To clarify the clinical features of ulcerative colitis with hypozincemia and the usefulness of Zinc replacement therapy.</p><p><b><i>Materials and Methods</i></b>: Ninety-six UC patients, median age 51 years old, whose serum Zinc levels were measured between 2022 and 2024 were retrospectively enrolled. The Zinc <80 μg/dl was classified as hypozincemia group(LG), and Zinc ≥80 as normal group(NG). Twenty-eight patients in the LG who underwent Zinc supplementation were evaluated for disease activity after supplementation. Patient background (age, gender, extent of disease, relapse rate), CRP, F-cal, pMayo score, and Mayo score were examined.</p><p><b><i>Results:</i></b> There were sixty-seven(70%)patients in the LG and twenty-nine(30%) in the NG. The median Zinc value of all patients was 73μg/dl. Median age (LG/NG) was 51/54 years old. Gender (male/female) was 33/34 in LG and 16/13 in NG. There was no difference in age, gender, extent of disease, relapse rate, CRP, or F-cal. The median pMayo score(LG/NG) was 1/0, p=0.004, and the median Mayo score(LG/NG) was 1/0, p=0.006, all significantly higher for LG. In the Zinc-supplemented patients who mainly used zinc acetate hydrate, the median values before and after Zinc supplementation were 69/101μg/dl , p=0.000, CRP 0.04/0.01 mg/dl, p=0.15, F-cal 95/63 mg/kg, p=0.22, pMayo score 1/0,p=1.0, Mayo score 2/0,p=0.31. Zinc levels were significantly improved after supplementation, but there were no differences in other parameters.</p><p><b><i>Conclusion:</i></b> The serum Zinc level may be a biomarker of UC activity. Short-term observation showed no change in disease activity with Zinc supplementation, further follow-up is needed.</p><p><b>PP-02-046</b></p><p><b>Plasma IgA Levels Correlate with Biological Therapy and ESR Levels in Patients with Ulcerative Colitis</b></p><p><b>Jye-lin Hsu</b> and Tsung-Yu Tsai</p><p><i>China Medical University, Taichung, Taiwan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Although IgA is the predominant antibody secreted by gut immune cells, the level of plasma IgA in ulcerative colitis (UC) patients undergoing biological treatment remains unknown. This study aimed to investigate the relationship between plasma IgA concentrations in UC patients receiving biological therapy.</p><p><b><i>Aim:</i></b> To investigate the relationship between plasma IgA levels in UC patients undergoing biological therapy.</p><p><b><i>Methods:</i></b> We enrolled 40 UC patients between 2020 and 2023. Plasma concentrations of IgG, IgM, and IgA were measured before initiating therapy, and at the 14th and 52nd weeks of biological therapy (Adalimumab, Vedolizumab, or Tofacitinib). We compared these antibody levels and their relationship with clinical manifestations. Statistical analysis was conducted using the Student's t-test.</p><p><b><i>Results:</i></b> Plasma IgA levels were significantly higher before initiating biological therapy and decreased after 14 and 52 weeks of treatment in UC patients. This pattern was not observed for plasma IgG and IgM levels. Additionally, plasma IgA levels significantly correlated with ESR levels (P=0.0275, R²=0.1430). There was also a trend toward a positive correlation between plasma IgA levels and Mayo subscore, although more cases are needed to confirm this observation.</p><p><b><i>Conclusion:</i></b> Baseline plasma IgA levels correlate with biological therapy in UC patients and were associated with ESR levels. IgM and IgG did not exhibit similar patterns. These findings suggest that plasma IgA levels may serve as a potential biomarker for UC patients.</p><p><b>PP-02-047</b></p><p><b>TNF-Alpha Inhibitors And Demyelinating Disease In Crohn’s Disease Patient : A Case Report</b></p><p><b>Karunia Valeriani Japar</b><sup>1</sup>, Ignatius Bima Prasetya<sup>1,2</sup> and Rabbinu Rangga Pribadi<sup>3</sup></p><p><sup>1</sup><i>Faculty of Medicine Universitas Pelita Harapan, Kota Tangerang, Indonesia;</i> <sup>2</sup><i>Fellow of Gastroenterohepatology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, CiptoMangunkusumo Hospital, Central, Indonesia;</i> <sup>3</sup><i>Division of Gastroenterology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, CiptoMangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The growing prevalence of Crohn's disease (CD) highlights a significant trend, though epidemiological data remains incomplete. TNF-alpha inhibitors that is used in Chron’s disease can cause demyelinating symptoms.</p><p><b><i>Case Description:</i></b> We present a case of a 26-year-old female with Crohn's disease and neurological complications after being prescribed with adalimumab and infliximab, respectively. The patient initially responded well to the treatment, but over time, she began to experience a loss of strength and balance in her limbs. Brain Magnetic resonance imaging revealed a distribution pattern suggestive of demyelinating plaques, although not sufficient to diagnose multiple sclerosis. The physicians team stop all biologic agents due to suspected drug interaction causing neurological symptoms. The patient showed progressive improvement over a one-year period, eventually no longer exhibiting any demyelinating symptoms. This positive clinical outcome highlights the potential for significant neural recovery and underscores the effectiveness of the treatment protocol, which included oral methotrexate, sulfasalazine, and steroids.</p><p><b><i>Discussion:</i></b> The potential for TNF-alpha inhibitors in CD to cause demyelination is concerning and often underappreciated. The exact mechanisms underlying these neurological complications are not fully understood, but one theory suggests that TNF-alpha blockers may enhance demyelination by increasing the migration of peripheral autoreactive T cells into the CNS. Previous studies estimate the incidence of demyelination due to TNF-α inhibitors to be between 0.03% and 0.2% of patients receiving these treatments.</p><p><b><i>Conclusion:</i></b> Given these potential adverse effects, clinicians should exercise caution when administering TNF-alpha inhibitors.</p><p><b><i>Keywords:</i></b> Crohn's disease, Demyelinating disease, TNF-alpha inhibitors</p><p><b>PP-02-048</b></p><p><b>The Contribution of Dietary Glycation Advanced End-Products and Genetic Liability in Inflammatory Bowel Disease Risk</b></p><p><b>Fangyuan Jiang</b><sup>1,2</sup>, Wenjing Yang<sup>1,2</sup>, Yushu Cao<sup>1,2</sup>, Xianghan Cao<sup>1,2</sup>, Yu Zhang<sup>1,2,3</sup>, Lingya Yao<sup>1,2,3</sup> and Qian Cao<sup>1,2,3</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China;</i> <sup>2</sup><i>Inflammatory Bowel Disease Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China;</i> <sup>3</sup><i>Institute of Gastroenterology, Zhejiang University, Hangzhou, 310016, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Evidence suggests dietary advanced glycation end products (AGEs) promote oxidative stress and inflammation in the gastrointestinal tract. This study investigates the association between dietary AGE intake and the risk of inflammatory bowel disease (IBD).</p><p><b><i>Materials and Methods:</i></b> A total of 123,354 participants without IBD at baseline were included from the UK Biobank. Consumption of three common AGEs (Nε-carboxy-methyllysine [CML], Nε-carboxyethyllysine [CEL], and Nδ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine [MG-H1]) was estimated by matching 24-hour dietary questionnaires to a validated dietary AGE database. Cox proportional hazards regression models calculated the hazard ratio (HR) and 95% confidence interval (CI) of associations between dietary AGEs and IBD risk.</p><p><b><i>Results:</i></b> Over a median follow-up of 13.72 years, 671 participants developed IBD, including 192 with Crohn’s disease (CD) and 478 with ulcerative colitis (UC). Only CEL was associated with an increased risk of IBD (HR=1.09, 95% CI: 1.01-1.18, P=0.020) and CD (HR=1.18, 95% CI: 1.03-1.36, P=0.014), particularly in overweight, physically inactive, and non-smoking participants. For participants at high genetic risk of CD, HRs (95% CI) were 1.26 (1.00-1.57) for CML, 1.41 (1.12-1.76) for CEL, and 1.28 (1.01-1.62) for MG-H1 (all P < 0.05). None of the dietary AGEs were significantly associated with UC risk, regardless of genetic predisposition.</p><p><b><i>Discussion:</i></b> Dietary CEL was associated with an increased risk of IBD and CD, but not UC, especially in those with high BMI, low physical activity, and no smoking history. Further interventional studies are needed to explore the potential benefit of AGE restriction, particularly for individuals at high genetic risk of CD.</p><p><b>PP-02-049</b></p><p><b>Correlation of Hematologic Inflammation Biomarker With Ulcerative Colitis Disease Activity at RSUP Wahidin Sudirohusodo Makassar</b></p><p><b>Putri Nila Kandi</b><sup>1</sup>, Muhammad Luthfi Parewangi<sup>2,3</sup>, Amelia Rifai<sup>2,3</sup>, Fardah Akil<sup>2,3</sup>, Nu'man AS Daud<sup>2,3</sup>, Rini Rachmawarni Bachtiar<sup>2,3</sup> and Susanto Hendra Kusuma<sup>2,3</sup></p><p><sup>1</sup><i>Internal Medicine Specialist Program, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>3</sup><i>Centre of Gastroenterology-Hepatology, HAM Akil Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Ulcerative Colitis (UC) is a chronic inflammatory disease of gastrointestinal tract that use endoscopic to assess the disease activity. To determine UC, a comprehensive approach involving clinical, biochemical, histological, and endoscopic biopsy evaluations is essential. Several studies have been conducted to determine hematological markers that are easier and more affordable to perform. Erythrocyte Sedimentation Rate (ESR) and Neutrophil-Lymphocyte Ratio (NLR) have been identified as some of potential inflammatory hematological biomarkers. An elevated ESR or NLR level may suggest inflammatory bowel diseases. The aim of this study is to determine correlation between hematological inflammatory biomarkers, such as ESR and NLR with the degree of disease activity in ulcerative colitis.</p><p><b><i>Materials and Method:</i></b> This research is an observational analytical study with a cross-sectional approach. With statistical test used is chi-square correlation test. A retrospective database analysis study from ulcerative colitis patients diagnosed between January-Juny 2024 in Wahidin Sudirohusodo Hospital Makassar was conducted. Information on patients demography, laboratory, and endoscopic biopsy data were collected.</p><p><b><i>Results:</i></b> There are 91 subjects in total with 23 subjects was moderate to severe ulcerative colitis and 68 subjects was mild ulcerative colitis were included in this study. A chi-square test was performed and there is a significant correlation between NLR (P < 0.005) but no correlation with ESR (P > 0.005) to the UC disease activity.</p><p><b><i>Conclusion:</i></b> Neutrophyl-lymphocyte ratio is one of hematological inflammation biomarker that significantly correlated with the degree of disease activity in ulcerative colitis.</p><p><b>PP-02-050</b></p><p><b>Effects of Zanthoxyli Pericarpium on inflammation and network pharmacological mechanism analysis in DSS-induced colitis mice</b></p><p>Woo-Gyun Choi<sup>1</sup>, Daehwa Jung<sup>2</sup>, Sang Chan Kim<sup>3</sup>, Na Ri Choi<sup>1,4</sup> and <b>Byungjoo Kim</b><sup>1</sup></p><p><sup>1</sup><i>Department of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, South Korea;</i> <sup>2</sup><i>Department of Pharmaceutical Engineering, Daegu Hanny University, Gyeongsan, South Korea;</i> <sup>3</sup><i>College of Oriental Medicine Daegu Haany University, Gyeongsan, South Korea;</i> <sup>4</sup><i>Department of Korean Medical Science, Pusan National University School of Korean Medicine, Yangsan, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Inflammatory bowel disease (IBD) is a condition characterized by chronic inflammation of the intestines. Zanthoxyli Pericarpium (ZP) has long been utilized in traditional oriental medicine for managing intestinal disorders due to its known anti-inflammatory and antioxidant properties. The researchers aimed to investigate the components of ZP and their specific targets, as well as associated diseases. Additionally, we sought to explore the protective effects of ZP on the colon and uncover the underlying molecular mechanisms in treating IBD.</p><p><b><i>Materials and methods:</i></b> The study employed the TCMSP analysis platform and database to identify the compounds in ZP and their interactions with specific target genes related to IBD. Furthermore, the researchers conducted experiments using a murine model of colitis induced by dextran sodium sulfate (DSS) to assess the impacts of ZP.</p><p><b><i>Results:</i></b> The network pharmacological analysis revealed that 17 compounds in ZP target 20 genes associated with IBD, including AKT1, CASP3, CCL2, CLDN4, CRP, CXCL10, CXCL2, HMOX1, ICAM1, IFNG, IL10, IL1B, IL2, IL6, MPO, NFKBIA, PPARG, PTGS2, STAT1, and TNF. In the DSS-induced colitis model, ZP demonstrated a significant reduction in colonic epithelial damage caused by DSS. Moreover, ZP effectively reduced the levels of oxidative stress markers, such as iNOS and nitrotyrosine, indicating its antioxidant properties.</p><p><b><i>Conclusions:</i></b> The study provides evidence supporting the protective effects of Zanthoxyli Pericarpium (ZP) against DSS-induced colonic damage, primarily attributed to its anti-inflammatory and antioxidant properties. These findings suggest that ZP could be a potential candidate for further exploration as a treatment for inflammatory bowel disease (IBD).</p><p><b>PP-02-051</b></p><p><b>Correlation of Disease Activity, Fatigue, and Quality of Life in Patients with Ulcerative Colitis</b></p><p><b>Seung-Jun Kim</b></p><p><i>CHA Bundang Medical Center, Bundang-gu, Seongnam-si, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The aim of this study was to investigate the correlation of disease activity with fatigue and quality of life (QoL), as well as the IBDQ score as a surrogate disease monitoring marker.</p><p><b><i>Method:</i></b> A total of 30 patients with UC were enrolled in this study. The patients were asked to fill out questionnaires on the day they received colonoscopy. Disease activity was assessed by the Mayo score (MS), fatigue levels were measured by multidimensional fatigue inventory Korean version (MFI-K) questionnaire, and QoL was measured by inflammatory bowel disease questionnaire (IBDQ).</p><p><b><i>Results:</i></b> Patients with moderate to severe disease activity displayed significantly higher levels of general and physical fatigue (16.2 vs 18.1, p=0.001) and reduced activity (14.6 vs 13.0 p=0.034), while mental fatigue (18.6 vs. 18.3, p=0.781) and motivation (9.2 vs. 10.6, p=0.250) were not significantly correlated with disease activity. Furthermore, patients with moderate to severe disease activity had significantly lower IBDQ scores compared to those with remission to mild disease activity (116.0 vs. 190.4, p<0.001). Using the ROC curve, we established that when the IBDQ score was cut off at 173, the sensitivity for indicating moderate to severe disease activity was 89.5%, and the specificity was 90.9%.</p><p><b><i>Conclusion:</i></b> Impaired QoL and increased fatigue, especially in categories of general and physical fatigue and reduced activity, are significantly correlated with higher disease activity. And it is recommended to perform an endoscopic workup when the IBDQ score is above 173.</p><p><b>PP-02-052</b></p><p><b>Eight cases of ulcerative colitis with mesalazine intolerance</b></p><p><b>Shinya Kondo</b>, Masakazu Kitamura, Tatsuya Tanaka, Akihiro Ukai and Shinya Maruta</p><p><i>KKR Tokai Hospital, Nagoya, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Mesalazine is the first-line drug used for the initial treatment of ulcerative colitis (UC). Since only a few cases of mesalazine intolerance have been reported, we reviewed such eight cases.</p><p><b><i>Materials and Methods:</i></b> We investigated the clinical background and treatment course of eight patients with mesalazine-intolerant UC treated in our hospital from January 2016 to December 2023. The median age of the patients was 50.5 (16-77) years, and the male to female ratio was 6:2. All patients had new-onset UC and the types were: pancolitis (5), right-sided colitis (1), left-sided colitis (1), and proctitis (1). Six patients received multi-matrix system-mesalazine, one received pH-dependent mesalazine, and one received time-dependent mesalazine as first-line treatments.</p><p><b><i>Results:</i></b> The median number of days from the start of treatment to symptom exacerbation was 10 (8-360). The drug lymphocyte stimulation test was positive in five patients and negative in three. Remission was achieved in all patients with mesalazine discontinuation and remission-induction therapy: systemic glucocorticoid administration and granulocytapheresis, systemic glucocorticoids, steroid enema, Ustekinumab (steroid was ineffective), and mesalazine discontinuation only were 1, 1, 3, 1, 2, respectively. Remission maintenance therapy used azathioprine, ustekinumab, golimumab, steroid enema, and no medication in three, one, one, one, and two patients, respectively.</p><p><b><i>Conclusion:</i></b> Drug intolerance was considered on sudden worsening of UC symptoms approximately 10 days after the initial mesalazine treatment. Although intolerance rarely develops after a prolonged course of treatment, it is necessary to be cognizant of this possibility early in the use of mesalazine.</p><p><b>PP-02-053</b></p><p><b>Deep learning model using stool pictures discriminates UC patients from subjects with normal colonoscopy</b></p><p><b>O Seong Kweon</b> and Eun Soo Kim and Dong Won Woo and Kyeong Ok Kim and Sung Kook Kim and Hyun Seok Lee and Ben Kang and Yoo Jin Lee and Byung Ik Jang and Eun Young Kim and Hyeong Ho Jo and Yun Jin Chung and Han Jun Ryu and Soo Kyung Park and Ho Sang Yu and Sung Moon Jeong</p><p><i>Kyungpook National University School of medicine, Daegu, South Korea;</i> <i>Kyungpook National University Hospital, Daegu, South Korea;</i> <i>Yeungnam University College of Medicine, Daegu, South Korea;</i> <i>Keimyung University School of Medicine, Daegu, South Korea;</i> <i>Daegu Catholic University School of Medicine, Daegu, South Korea;</i> <i>Daegu Fatima Hospital, South Korea;</i> <i>Sungkyunkwan University School of Medicine, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Diagnosis of ulcerative colitis (UC) is challenging as there is no gold standard diagnostic tool of the disease.</p><p>We previously developed deep learning model using stool photos for predicting endoscopic activity in 306 patients with UC (DLSUC)</p><p>This study aimed to explore the potential of the DLSUC in the diagnosis of UC.</p><p><b><i>Materials and Methods:</i></b> Patients with endoscopically active UC and subjects for colonoscopy with various symptoms were prospectively enrolled in the study.</p><p>They were asked to take stool pictures with their smartphones 1 week before undergoing endoscopy.</p><p>Area under the receiver operating characteristic (AUC), sensitivity, specificity, and accuracy to discriminate UC patients from subjects without UC were estimated.</p><p><b><i>Results:</i></b> The deep learning model for screening ulcerative colitis patients, demonstrated an accuracy of 0.8718, a sensitivity of 0.80636, and a specificity of 0.8824, outperforming fecal calprotectin, which showed an accuracy of 0.7941, a sensitivity of 0.8095, and a specificity of 0.7692</p><p>In validation set, area under curve (AUC) for predicting endoscopic activity using AI model was 0.914 which was higher, but not statistically different from AUC using Fcal (0.82, in previous study)</p><p><b><i>Conclusion:</i></b> Stool photos of UC patients with deep learning technology demonstrated similar accuracy to Fcal in discriminating active UC patients from subjects with normal colonoscopy.</p><p>Deep learning model showed sufficient accuracy differentiating IBS patients with UC patients.</p><p>Artificial intelligence and smartphone technology using stool photos showed potentials as one of the adjunctive tools for assessing mucosal inflammation of UC patients and screening of suspected UC patients.</p><p><b>PP-02-054</b></p><p><b>Multi-omics phenotyping characterizes perturbed molecular divergence underlying different clinical scenarios of under-treatment inflammatory bowel disease</b></p><p><b>Hongsub Lee</b></p><p><i>Inje University College of Medicine, Busan, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>Clinically heterogeneous spectrum and molecular phenotypes of inflammatory bowel disease (IBD) remain to be comprehensively elucidated. The serum proteome, metabolome, and lipidome of 75 IBD patients were profiled. Compared to Crohn’s disease, ulcerative colitis showed a lower abundance of hypoxanthine correlating negatively with cell adhesion-related proteins of ITGB2, CD200, PRELP, and positively with lysophosphatidylcholines [LPC(16:0-SN1), LPC(O-18:1)]; and up-regulation of bile acids and FGF19. For (II), while proteins related to inflammatory-promoting signaling pathways, sphingomyelins (SMs), and LPC were increased; bile acids, amino acids, and triacylglycerols (TGs) were decreased. Not only in (II), TGs were a major class change in (III), whose abundance was consistently higher in the active state. This, along with decreased SMs levels, correlated strongly with the up-regulation of inflammatory-inducing proteins (CXCL9, CXCL1, ITGB2, IL17A). In (IV), treatment escalation promoted augmentations of signaling pathways-related proteins and triggered significant metabolome responses, especially decreased amino acids. Noticeably, the levels of TGs were consistently increased in the post-treatment escalation group, thereby suggesting treatment response with relation to (III). For (V), down-regulation of signaling pathways-related proteins and higher levels of SMs and PCs was observed. A consistent decrease of TGs in patients who needed treatment escalation along with lower level of TGs in elevated calprotectin group could imply for the inflammatory level. This study provided the multi-ome profiles of IBD patients in five clinical scenarios and laid a foundation for opening new doors for disease management and treatment.</p><p><b>PP-02-055</b></p><p><b>Prevalence of Inflammatory Bowel Disease in Kinta Valley, Malaysia: An Update after a Decade</b></p><p><b>Hak Keith Leung</b><sup>1</sup>, Xin-Hui Khoo<sup>1</sup>, Yi Wern Khong<sup>1</sup>, Suh Yu Cheong<sup>2</sup>, Phei Onn Tan<sup>2</sup>, Andrew Seng Boon Chua<sup>3</sup>, Andrew Seng Boon Chua<sup>4</sup>, Harjinder Singh<sup>5</sup>, Thean Heng Ng<sup>5</sup>, Choon Heng Wong<sup>6</sup>, Wai Hong Kong<sup>6</sup> and Ida Normiha Hilmi<sup>1</sup></p><p><sup>1</sup><i>University Of Malaya, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Hospital Raja Permaisuri Bainun, Ipoh, Malaysia;</i> <sup>3</sup><i>Ipoh Gastro Centre, Ipoh, Malaysia;</i> <sup>4</sup><i>Hospital Fatimah, Ipoh, Malaysia;</i> <sup>5</sup><i>Pantai Hospital Ipoh, Ipoh, Malaysia;</i> <sup>6</sup><i>KPJ Specialist Centre, Ipoh, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aimed to update Malaysia's current prevalence of inflammatory bowel disease.</p><p><b><i>Methods:</i></b> All IBD cases in five hospitals in Kinta Valley were recruited retrospectively, and attempt was made to contact all resident cases who lost follow-up. Uncontactable cases were put in request to National Registry Department to inquire on death registration. The demographics and clinical characteristic were obtained from medical records.</p><p><b><i>Results:</i></b> 153 case notes of IBD patients in Kinta valley were reviewed. Six patients had deceased. Two patients were uncontactable. 145 confirmed resident cases of IBD was identified. Among them, there were 56.6% males, 43.4% females; 29.0% Malays, 32.4% Chinese, 38.6% Indians. Median age of diagnosis was 38.0(25.0-52.0). The distribution of the cases was as follows: 102 UC(70.4%), 42 CD(28.9%) ,1 IBD-U(0.7 %). Among CD patients, there were 58.1% non-stricturing non-penetrating cases, 35.5% stricturing cases, 9.6% penetrating, and 25.0% perianal disease. The disease location was 29.0% terminal ileum, 35.5% colonic, 35.5% ileocolonic, 12.9% isolated upper disease. Among UC patients, 32.2% were proctitis, 35.6% were left-sided, 32.2% were extensive. The prevalence rates were 16.31, 11.48, 4.73, 0.11 for IBD, CD, UC and IBD-U per 100,000 persons respectively. The highest prevalence rate was among Indians(50.77 per 100,000 person), followed by Chinese(14.61 per 100,000 person) and Malays(10.43 per 100,000 persons).</p><p><b><i>Conclusions:</i></b> There was an increased prevalence of IBD cases in Kinta valley compared to a decade ago. There is also reversal of prevalence between Chinese and Malay population, whereby there were more Chinese IBD patients diagnosed within the decade.</p><p><b>PP-02-057</b></p><p><b>Luminal Zinc Induces Clostridioides difficile Virulence and Exacerbates Gut Inflammation in UC patients</b></p><p><b>Jie Liang</b> and Tong Wu</p><p><i>Xijing Hospital, Xi'an, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The disease severity of Ulcerative Colitis (UC) is often complicated by C. difficile infection (CDI). However, the actual role of C. difficile in UC development remains unknown.</p><p><b><i>Methods:</i></b> UC patients’ stool samples (n=60) were collected and analyzed by qPCR and ICP-MS to determine the presence of C. difficile and the fecal zinc concentrations. C. difficile was cultured in BHIs with or without supplemented zinc in an anaerobic chamber. Expression of virulence genes was analyzed and biofilm assays were used to examine C. difficile flagellar formation. Human colonic organoids and Caco-2 cells were co-cultured with C. difficile or zinc-treated C. difficile, and cytotoxicity, permeability, and inflammatory cytokine release were assessed. TLR5 knock-out Caco-2 cells were constructed and co-cultured with C. difficile or zinc-treated C. difficile to verify whether zinc-induced C. difficile’s pathogenicity was mainly through flagellar formation.</p><p><b><i>Results:</i></b> C. difficile was detected in ~35% of UC patients’ fecal samples. Luminal zinc concentrations (~915μM, p<0.01) were significantly higher among UC patients than CD patients or healthy subjects. The UC patients’ luminal zinc concentration promoted C. difficile growth, suppressed its exotoxins release, and induced higher flagella-related gene expression and flagellar formation, resulting in higher cytotoxicity and inflammatory responses in human colonic organoids and Caco-2 cells. Also, zinc-treated C. difficile did not induce higher inflammation and pathogenicity in TLR5 knock-out Caco-2 cells.</p><p><b><i>Conclusion:</i></b> The association between C. difficile and UC aggravation may partially stem from UC patients’ excess luminal zinc, which promotes C. difficile flagellar formation, induces inflammatory responses, and worsens UC progression.</p><p><b>PP-02-058</b></p><p><b>SLC7A5 promotes the progression of UC by regulating amino acid transport and intestinal epithelial autophagy</b></p><p><b>Jie Liang</b> and Yanting Shi</p><p><i>Xijing Hospital, Xi'an, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Inhibition amino acid metabolism of inflammatory sites may be effective in alleviating UC (ulcerative colitis). SLC7A5 is involved in the progression of UC, but the mechanism of its functional regulation still needs to be investigated.</p><p><b><i>Methods:</i></b> We previously discovered through high-throughput screening that SLC7A5, as a cellular amino acid transport carrier, is significantly elevated in the colonic tissues of UC patients. This study utilized model animals, transcriptomics, amino acid-targeted metabolomics assays, and macro-genome sequencing to elucidate the mechanism of SLC7A5-regulated amino acids in mTOR pathway activation and intestinal autophagy dysfunction.</p><p><b><i>Results:</i></b> SLC7A5 expression is increased in the colon of UC patients and DSS-induced mice lesions. SLC7A5 inhibitor (JPH203) restrained the inflammatory responses induced by DSS. SLC7A5 deletion or inhibition dampens the release of IL-1β, IL-18, and IL-23 and the production of ROS in the LPS-induced FHC and RAW264.7 cells. Moreover, upregulating SLC7A5 expression induces mTOR signaling pathway activation in FHC cells. Deletion or inhibition of SLC7A5 efficiently blocks the SLC7A5-dependent amino acid transport, inhibits the mTOR activation, and results in the activation of autophagy.</p><p><b><i>Conclusion:</i></b> Targeting SLC7A5-mediated amino acid uptake is a potentially useful immunosuppressive strategy to regulate colonic inflammation through mTOR pathway and autophagy. This study is expected to reveal the intrinsic factors of metabolic disorders promoting the UC progression from the perspective of amino acid metabolism, and to lay a new theoretical and experimental foundation for potential UC treatment.</p><p><b>PP-02-059</b></p><p><b>Desulfovibrio flagellin promotes H3K27 histone methylation modification in intestinal epithelial cells inducing inflammatory bowel disease</b></p><p><b>Xiang Liu</b>, Ge Jin and Hailong Cao</p><p><i>Department Of Gastroenterology And Hepatology, General Hospital, Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The surge in inflammatory bowel disease (IBD) is closely related to the dysbiosis of the intestinal microbiota and the disruption of the intestinal barrier. We found that Desulfovibrio vulgaris fagellin (DVF) enhanced histone H3K27 methylation (H3K27me3), decreased the expression of tight junction protein ZO-1 and disrupted the intestinal barrier, and H3K27 methylase screening showed that the expression of PRC2 core subunit-EED was increased. Therefore, we hypothesized that DVF inhibits ZO-1 expression by promoting EED-mediated H3K27 methylation, thereby disrupting the tight junction of the intestinal epithelium.</p><p><b><i>Materials and Methods:</i></b> The correlation between Desulfovibrio vulgaris (DSV) abundance, EED expression and intestinal inflammatory activity was investigated in IBD patients. Cell transfection, EED inhibitors and CUT&Tag experiments were used to explore the mechanism of DVF regulating the EED-H3K27me3-ZO-1 axis.</p><p><b><i>Results:</i></b> Disease activity in UC patients was positively correlated with DSV abundance and EED expression. After DVF stimulation of Caco2 cells, the expression of ZO-1 protein decreased and the expression of inflammatory factors increased. EED expression was knocked down in Caco2 cells, and ZO-1 and inflammatory factor expressions were unchanged. CUT&Tag sequencing of stimulated Caco2 cells confirmed that the expression of H3K27me3 was increased and ZO-1 expression was inhibited. After DVF stimulation in mice, the expression of intestinal inflammation increased, the expression of H3K27me3 and EED increased, and the expression of ZO-1 decreased. This change can be eliminated with the inhibitor of EED226.</p><p><b><i>Conclusion:</i></b> DVF promotes H3K27 histone methylation modification in intestinal epithelial cells and destroys the intestinal barrier to induce IBD.</p><p><b>PP-02-060</b></p><p><b>Comparative efficacy and safety of therapies for adult ulcerative colitis: a network meta-analysis</b></p><p>Zilin Ma</p><p>17735972555, Zikai Wang</p><p>Xiaoyan Chi</p><p>13467853456, Fengqi Liu, Rongrong Ren</p><p><i>PLA General Hospital, Beijing, China</i></p><p><b><i>Objectives:</i></b> To evaluate the efficacy and safety of mesalazine, glucocorticoids, immunosuppressants, small-molecules, biologics, and fecal microbiota transplantation in treating UC in adults.</p><p><b><i>Materials and Methods:</i></b> Literature search in Cochrane, Embase, and PubMed databases up to June 2024 identified RCTs involving adult UC patients treated with the aforementioned therapies. Clinical remission and mucosal healing were the primary efficacy outcomes, with adverse events (AEs) and serious AEs as the safety outcomes. A network meta-analysis was conducted using a multivariate consistency model and random-effect meta-regression, with treatment rankings based on the surface under the cumulative ranking curve (SUCRA). Cluster analysis was applied to assess the SUCRA scores for efficacy and safety, identifying the optimal treatment.</p><p><b><i>Results:</i></b> 71 RCTs, 10,338 UC patients included. Tacrolimus showed the highest clinical response rate (RR 0.25, 95% CI 0.06-0.57; SUCRA, 93.7%) and mucosal healing. Mercaptopurine (6-MP) had the highest rank for clinical remission (RR=0.63, 95%CI 0.55-0.86; SUCRA, 85.8%) but the lowest for AEs (RR=1.25, 95%CI 1.20-1.80; SUCRA, 14.2%). Only Vedolizumab reduced AEs, with the highest adverse event ranking (RR=0.38, 95%CI 0.25-0.79; SUCRA, 95.1%). Infliximab uniquely increased the risk of serious AEs (RR=1.70, 95%CI 1.52-1.96). Cluster analysis of clinical response and AEs indicated Vedolizumab as the most efficacious treatment for UC (Figure 1). The study's conclusions should be cautiously interpreted due to potential biases and heterogeneity.</p><p><b>PP-02-061</b></p><p><b>Comparing TRIple Rectal Ultrasound Imaging Technology in Ulcerative Colitis(TRINITY Study):Trans abdominal, perineal and Endoscopic ultrasound</b></p><p><b>Partha Pal</b>, Mohammad Abdul Mateen, Anuradha Sekaran, Kanapuram Pooja, Jahangeer Basha, Rajesh Gupta, Manu Tandan, Sundeep Lakhtakia and D Nageshwar Reddy</p><p><i>Asian Institute Of Gastroenterology, Hyderabad, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To compare the accuracy of transperineal ultrasound (TPUS) with endoscopic ultrasound (EUS), endoscopic activity, and histologic activity in assessing rectal inflammation in ulcerative colitis (UC).</p><p><b><i>Methods:</i></b> Consenting UC patients (18-75 years) underwent trans-abdominal ultrasound (TAS), TPUS, sigmoidoscopy (with biopsy), and EUS in the same session to correlate rectal total wall thickness (TWT) and vascularity across TAS, TPUS, and EUS with endoscopic and histologic activity.</p><p><b><i>Results:</i></b> 49 UC (median age 38 years, 57.1% male) were included. The area under the curve (AUC) to predict endoscopic activity was highest for EUS [mucosal thickness>0.95mm, sensitivity(Sn): 95%, specificity(Sp): 85%, AUC: 0.93; TWT >3.9 mm: AUC: 0.79), vascularity (Modified Limberg score-MLS)> 1: AUC: 0.85, submucosal thickness (SMT)>2.1mm: AUC: 0.77) followed by TPUS (TWT>5.7 mm: AUC: 0.619, MLS>1: AUC: 0.62) and TAS (TWT >6.4 mm: AUC: 0.50; MLS>1: AUC: 0.5) (Figure 1A; p values, 95% CI, 1B-C: Receiver operating characteristic curves).</p><p>Prediction of histologic activity (Nancy Index) (n=26) was best with EUS (MLS≥ 1; Sn: 90%, Sp: 80%; AUC: 0.91; SMT >2.1mm: AUC: 0.75; MT >1mm: AUC: 0.72; TWT >3.9 mm: AUC: 0.64) followed by TPUS (TWT >5.6 mm: AUC: 0.77; MLS ≥1: AUC: 0.67) and TAS (TWT >6.4 mm: AUC: 0.54; MLS ≥1: AUC: 0.57) (Figure 1D,p value,95%CI, E-F: ROC curve).</p><p><b><i>Conclusion:</i></b> TPUS shows promise in assessing rectal inflammation in UC but is less accurate than EUS for predicting endoscopic and histologic remission. EUS remains the gold standard for evaluating rectal inflammation, while TPUS can serve as a complementary, non-invasive tool with moderate accuracy.</p><p><b>PP-02-063</b></p><p><b>Flow of Advanced Therapy Pathways and Variations over Time in Crohn’s Disease and Ulcerative Colitis</b></p><p><b>Fei Yang Pan</b><sup>1,4</sup>, Aviv Pudipeddi<sup>2,3</sup>, John Chetwood<sup>2,3</sup> and Rupert Leong<sup>2,3</sup></p><p><sup>1</sup><i>Macquarie University Hospital, Sydney, Australia;</i> <sup>2</sup><i>University of Sydney, Concord, Australia;</i> <sup>3</sup><i>Concord Repatriation General Hospital, Concord, Australia;</i> <sup>4</sup><i>Macquarie University, Sydney, Australia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The choice of advanced therapy (AT) in Crohn's disease (CD) and ulcerative colitis (UC) has increased, but the optimal sequencing of agents is unknown. We present the first national data on AT treatment sequencing in Australia using Sankey diagrams.</p><p><b><i>Methods:</i></b> We analysed the Persistence Australian National IBD Cohort (PANIC) 5 registry, including UC and CD patients on AT via the Pharmaceutical Benefits Scheme up to December 2021. Sankey diagrams were created using Plotly. Statistical analyses were performed using SPSS.</p><p><b><i>Results:</i></b> Among 9,671 UC patients (23,220 patient-years), VED patients were more likely to stay on treatment compared to TNFi (P<0.001). TOF was less likely to be used in bio-naïve patients and more commonly in bio-exposed patients. TOF increased from 85 to 397 patients (366%). UC patients were 13.1 times (P<0.001) more likely to stop treatment in T1 compared to T3.</p><p>Among 19,087 CD patients (79,677 patient-years), VED patients were more likely to stay on treatment compared to TNFi (P<0.001). UST was 2.5 times more likely to be used in bio-exposed patients compared to ADA. CD patients were 13.6 times (P<0.001) more likely to stop treatment in T1 compared to T3. UST increased from 2,231 to 4,294 patients (92%), and VED from 1,171 to 1,690 patients (44%).</p><p><b><i>Conclusion:</i></b> This study highlights diverse AT pathways for IBD patients, leading to greater treatment retention over time, especially for TOF and UST compared to IFX, ADA, VED, and GOL. There is an increasing trend of TOF and UST use as salvage therapy.</p><p><b>PP-02-064</b></p><p><b>Intestinal Behçet’s disease: clinical features and management In a Korean tertiary hospital</b></p><p><b>Subum Park</b>, Cheolmin Lee and Cheolwoong Choi</p><p><i>Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Behçet’s disease(BD) is an inflammatory vasculitis disease characterized by recurrent oral and genital ulcers, ocular inflammation, and skin lesions. Behçet’s patients can also present with gastrointestinal lesions. Although gastrointestinal symptoms in BD are noted in half, intestinal-BD is rare. We evaluated the clinical features and treatment of patients with intestinal-BD at a single center in Korea.</p><p><b><i>Methods:</i></b> 327 patients who met the international study group criteria for BD, followed up in a tertiary center from November 2008 to September 2023 were evaluated in this medical records review study. Among 327 BD patients, 261 were BD without GI involvement, and 66 intestinal-BD patients were enrolled. Data of demographics, clinical manifestations, laboratory and treatment were assessed and compared between two groups.</p><p><b><i>Results:</i></b> The mean age of 327 BD patients was 43.42years.</p><p>In laboratory findings, HLA-B51 demonstrated a significant difference.</p><p>In clinical manifestations, uveitis, skin lesions, arthritis, abdominal pain and GI bleeding showed statistical significance.</p><p>In treatment, 5-ASA, azathioprine and adalimumab demonstrated a significant difference.</p><p>Intestinal BD showed higher in operations and hospitalization compared to BD.</p><p><b><i>Conclusions:</i></b> This cohort of Korean patients with intestinal BD showed a predominance of females, although not statistically significant. Laboratory findings indicated a lower prevalence of HLA-B51 among intestinal BD patients, and clinical manifestations revealed a lower incidence of uveitis, skin lesions, and arthritis. In terms of treatment, we observed a higher frequency of 5-ASA, AZA, and adalimumab in intestinal BD, and noted a higher rate of surgery and hospitalization in patients with intestinal BD.</p><p><b>PP-02-065</b></p><p><b>Inflammatory Bowel Disease Patients need Higher Opioid Analgesic for Intravenous Conscious Sedation for Colonoscopy.</b></p><p><b>Young Sook Park</b>, Gyeol Song, Sang Bong Ahn and Young Mi Jeon</p><p><i>Eulji University/ Nowon Eulji University Hospital, Seoul, Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Patients with inflammatory bowel disease (IBD) require colonoscopy for diagnosis, disease activity assessment, and dysplasia surveillance. Patients with IBD reported lower satisfaction with sedation during colonoscopy and increased procedural pain compared with individuals without IBD. This study aimed to examine sedation requirements of IBD patients undergoing colonoscopy.</p><p><b><i>Materials and Methods:</i></b> A retrospective cohort study of IBD and non-IBD patients presenting for colonoscopy between January 2017 and December 2021 was undertaken. Data collected included patient and procedure focused variables. Sedation was performed as intravenous conscious sedation (IVCS) using midazolam and propofol. The opioid analgesic, pethidine was added If the sedation level was not adequate for procedures.</p><p><b><i>Results:</i></b> A total of 1655 consecutive colonoscopies (253 IBD, 1402 non-IBD) were analyzed. For IVCS, 11.1% of IBD patients required Midazolam only(M), 41.9%, Midazolam and propofol (MP), and 47.0%, Midazolam, Propofol and opioid (MPO) versus 11.7% of non-IBD required M, 69.1% MP and 19.2% MPO respectively. Among IBD patients (CD 52, UC 201), active disease state required MPO in 51,6% (66/128) versus inactive state 42,4% (53/125) (p=0.18). Propofol administration (mg) was 25.4±13.3 in IBD and 23.7±12.7 in non-IBD (p=0.07).</p><p><b><i>Conclusion:</i></b> IBD patients required more opioid analgesic for IVCS compared with non-IBD patients. IBD patients showed similar opioid requirement both active and inactive disease state.</p><p><b>PP-02-066</b></p><p><b>Assessment of anemia in patients with inflammatory bowel disease and its correlation with worse outcomes</b></p><p><b>Viviana Parra-izquierdo</b> and Ginary Orduz and Juan Frias and Silvia Hoyos and Juliep Sarmiento and Oscar Pinto and Alexis Duarte and Carlos Cuadros and Johon Garces and Andres Barco and Javier Acevedo and Javier Fonseca and Cristian Florez and Javier Fonseca</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> To describe the presence and type of anemia in patients with Inflammatory Bowel Disease (IBD) and its relation with relapses, related complications and disease and its relationship with relapses of the disease, complications related to this and the need to start need for initiation of biologic therapy.</p><p><b><i>Materials and Methods:</i></b> Cross-sectional, descriptive observational study of patients with patients with Inflammatory Bowel Disease who have been admitted to the Center of Excellence and have presented with anemia during the last 3 months of their disease, establishing its relationship with relapses, complications and the use of biologic therapy.</p><p><b><i>Results:</i></b> 46 patients evaluated, 40% of whom had anemia, of whom 59% were patients with ulcerative colitis and 41% with Crohn's disease. 59% were patients with ulcerative colitis and 41% patients with Crohn's disease. For the group of patients with ulcerative colitis group of patients with ulcerative colitis, it was found that 80% had anemia of chronic disease characteristics, 10% anemia due to chronic disease, and 10% anemia due to Crohn's disease. disease, 10% with anemia due to vitamin B12 deficiency and 10% with anemia in the context of mileodysplastic syndromes.</p><p><b><i>Conclusions:</i></b> Early diagnosis of anemia in patients with inflammatory bowel disease is essential.</p><p>Inflammatory Bowel Disease, it was demonstrated in our group of patients its relation with the extent and severity of the of the disease, as well as the increase in the number of relapses and the requirement of biologic therapy, including combination therapy.</p><p><b>PP-02-067</b></p><p><b>Translation and implementation IBD-F fatigue scale in a Colombian center for IBD: an observational study.</b></p><p><b>Viviana Parra-izquierdo</b> and Juan Frias and Ginary Orduz and Silvia Hoyos and Andrea Reatiga and Juliep Sarmiento and Luis Bohorquez and Oscar Pinto and Hernan Duarte and Carlos Cuadros and Johon Garces and Juan Acevedo and Andres Barco and Manuel Ardila and Cristian Florez</p><p><i>Leuven University, Bucaramanga, Colombia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Fatigue is a frequent symptom associated with inflammatory bowel disease (IBD). In Colombia, there is no history of fatigue assessment in patients with IBD, and an initial approach is required. The objective of this study was to evaluate IBD patients with IBD in a reference center for IBD in Colombia with the IBD fatigue scale (IBD-F).</p><p><b><i>Materials and methods:</i></b> Descriptive observational cross-sectional study in patients with IBD in a Colombian reference center, during the year 2023, using the IBD-F scale.</p><p><b><i>Results:</i></b> Thirty-two subjects were included, predominantly men (53.1%), mean age 40.1 years (SD 15.6; range 17-81), all in disease remission, and history of disease activity in the last 2 years, 62.5% with ulcerative colitis. At the time of the survey, in 53.1% the level of fatigue was zero. When evaluating average fatigue in the last 2 weeks, in 59.5% gave a score >1. In terms of fatigue impact, 46.8% had to take a nap during the day because of fatigue, and in 37.4% fatigue affected the ability to do normal household activities, and 37.5% had their quality of life affected by fatigue. Disease activity was the main cause related to fatigue.</p><p><b><i>Conclusions:</i></b> Assessment of fatigue by IBD-F is comprehensive, disease-focused, and useful in clinical practice, and should be part of comprehensive management in IBD.</p><p><b>PP-02-068</b></p><p><b>Digital-chromoendoscopy is associated with increased detection of colorectal cancer in Latin-American patients with ulcerative colitis</b></p><p><b>Viviana Parra-izquierdo</b> and Juan Frias and Edgar Ibañez and Fabian Juliao and Fabio Gil and Hernando Marulanda and Lina Otero and Elder Otero and Fabian Puentes and Lazaro Arango and Gerardo Guzman and Kenneth Suarez and Keyla Villa and Juan Paredes and Maria Jara and David Andrade and Cristian Florez and Abel Sachez and William Otero-Regino</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The prevalence of colorectal cancer in patients with ulcerative colitis is higher than in the general population, in Latin America there is progressive increase of Ulcerative Colitis and information on screening for colorectal cancer in inflammatory bowel disease in our region is scarce.</p><p><b><i>Objective:</i></b> The main objective of this study is to analyze the findings of endoscopic surveillance of colorectal cancer in patients with ulcerative colitis in Latin America according to the available endoscopic technology.</p><p><b><i>Materials and Methods:</i></b> Cross-sectional, multicenter, analytical study, performed in Latin American countries, in patients with Ulcerative Colitis with more than 8 years of diagnosis or with association with primary sclerosing cholangitis. Surveillance colonoscopies were performed according to available technology. Risk factors for dysplasia detection were analyzed.</p><p><b><i>Results:</i></b> 144 patients, 55.5% women, mean age 47.3 (range 17.1-90; SD 15.64) years and mean time of disease 12.71 (range 0.64-57.13; SD 8.08) years, in whom 49 lesions were identified, 18 corresponded to dysplasia. The detection rate of dysplasia per lesion and per procedure was 36.7% and 12.5%, respectively. By logistic regression analysis, time of disease OR 1.12; 95%CI: 1.047-1.215, p=0.002) and the presence of pseudopolyps (OR 3.4; 95%CI: 1.11-10.36, p=0.031) were the risk factors for higher detection of dysplasia. Digital chromoendoscopy was associated with greater detection of dysplasia (OR 4.99, 95%CI 1.092-22.864, p=0.038) than white light.</p><p><b><i>Conclusions:</i></b> This is the first Latin American study evaluating real-world experience in surveillance of dysplasia and colorectal neoplasia in patients with ulcerative colitis.</p><p><b>PP-02-069</b></p><p><b>Breaking barriers in the pediatric patient witi ibd transition process</b></p><p><b>Viviana Parra-izquierdo</b> and Ginary Orduz and Juan Frias and Carlos Cuadros and Andres Reatiga and Juliep Sarmiento and Luis Bohorquez and Diego Cano and Oscar Pinto and Hernand Duarte and Juan Aceved and John Garces and Andres Barco and Cristian Florez</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The diagnosis of Inflammatory Bowel Disease (IBD) includes any stage of life, and being a chronic disease, it is necessary to be accompanied by a multidisciplinary group of specialists, as well of specialists, as well as the family nucleus and an adequate social environment.</p><p><b><i>Materials and Methods:</i></b> A descriptive cross-sectional study describing the implementation of the pediatric implementation of the pediatric transition protocol in patients of the Center of excellence in Inflammatory Bowel Disease.</p><p><b><i>Results:</i></b> 10 patients, with a minimum age of 7 years and a maximum age of 17 years, of which 50% were diagnosed with ulcerative colitis and 50% with Crohn's disease. 70% were female. Regarding treatment, 60% required biologic therapy, with a higher percentage for the use of Infliximab, followed by Adalimumab with 33.3% and 2 cases of combined Ustekinumab + Ustekinumab + Adalimumab with 33.3%, and another case of Vedolizumab + Upadacitinib. In 30% with requirement of corticosteroids. In terms of the need for hospitalization, 70% of the pediatric population of the Center of Excellence required hospitalization due to disease outbreak. 20%, due to age, were admitted to the pediatric transition protocol , and social and family barriers were identified in 100% of them. For which a strict follow-up was carried out with the nurse, gastroenterologist and the nurse, the adult and pediatric gastroenterologist.</p><p><b><i>Conclusions:</i></b> there is a need for a pediatric transition protocol that establishes the importance of taking into account the main changes in the pediatric patient.</p><p><b>PP-02-070</b></p><p><b>Experience of accelerated induction with infliximab in a center of inflammatory bowel disease in colombia</b></p><p><b>Viviana Parra-izquierdo</b> and Sergio Romero and Carlos Cuadros and Juan Frias and Johon Garces and Silvia Hoyos and Ginary Orduz</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Infliximab treatment has proven be effective in inducing and maintaining remission in patients with high-risk or high-risk patients with high-risk or severe inflammatory bowel disease, which is why accelerated induction therapy at higher doses and at shorter intervals has been and shorter intervals has been proposed as a strategy to achieve a more rapid and complete therapeutic response in patients with active disease.</p><p><b><i>Objective:</i></b> To describe the clinical and paraclinical characteristics and outcomes of 5 patients with inflammatory bowel disease who received accelerated induction therapy with Infliximab.</p><p><b><i>Materials and Methods:</i></b> Retrospective cross-sectional descriptive study in which the clinical, and paraclinical characteristics and outcomes of 5 patients with inflammatory bowel disease who were patients with inflammatory bowel disease.</p><p><b><i>Results:</i></b> 5 patients with severe acute inflammatory bowel disease, refractory to other therapies other therapies, who received accelerated induction therapy with varying doses and intervals of infliximab, 3 with Crohn's disease and 2 with ulcerative colitis, of which 20% (1 patient with Crohn's disease) achieved clinical remission, 80% (1 patient with Crohn's disease) achieved achieved clinical remission, 80% (4 patients) did not achieve induction of remission, 60% (3 patients) required alternative therapies for symptom control but only 20% achieved remission following the addition of small molecule therapy.</p><p><b><i>Conclusions:</i></b> it is evident that the results are not satisfactory, this treatment strategy is not effective, however, it is still an option in acute, very severe and refractory cases of the disease.</p><p><b>PP-02-071</b></p><p><b>Real-world experience with intestinal ultrasound use in colombian patients with ibd: an observational study</b></p><p><b>Viviana Parra-izquierdo</b> and Samuel Cubillos-rodriguez and Melquisedec Vargas and Edgar Ibañez and Silvia Rios and Ginary Orduz and Silvia Hoyos and Cristian Florez</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Intestinal ultrasound is a new tool in assesing IBD activity. Our objective is to describe the ultrasonographic findings in patients with Ulcerative Colitis and Crohn's Disease, as well as to evaluate the Milan Ultrasound Criteria to evaluate the Milan Ultrasound Criteria (MUC) as a predictor of activity and its correlation with disease activity in disease activity in Colombian patients with IBD</p><p><b><i>Materials and Methods:</i></b> Comparative study, in patients with Inflammatory Bowel Disease who were evaluated with Intestinal Ultrasound.</p><p><b><i>Results:</i></b> 46 subjects with inflammatory bowel disease, 40 with ulcerative colitis and 6 with Crohn's disease, both adults and pediatric, predominantly male, were included.</p><p>In subjects with ulcerative colitis, the colonic wall thickening (CWT) median (CWT) in patients in endoscopic remission (Mayo score 0-1) were 2.52 (RIQ 2.1 - 2.82) mm compared to 3.5 (RIQ 2.1 - 2.82) mm in patients in endoscopic remission (Mayo score 0-1). 2.82) mm compared to 3.12 (RIQ 2.18-4.2) mm for patients with endoscopically active disease (Mayo 2). In subjects with Crohn's disease, in 4/6 there was evidence of a increase of more than 3mm in the wall thickness, as for the Limberg scale, 4 patients in grade 1, 1 in grade 2 and 1 in grade 3.</p><p><b><i>Conclusions:</i></b> Intestinal Ultrasound in patients with Ulcerative Colitis and Crohn's Disease is a useful and effective tool in the evaluation of disease activity in Ulcerative Colitis and Crohn's Disease.</p><p><b>PP-02-072</b></p><p><b>Characterization of patients with IBD and extraintestinal manifestations from Colombia: an observational study</b></p><p><b>Viviana Parra-izquierdo</b> and Juan Frias and Juan Marquez and Fabian Puentes and Fernando Sarmiento and Rafael Garcia and Melquisedec Vargas and Gustavo Reyes and Manuel Ardila and Carolina Samper and Cristian Florez</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Information about extraintestinal manifestations (EIM) in inflammatory bowel disease (IBD) is scarce in Latin America and its implications are unknown. The aim of this study was to describe demographic characteristics, clinical presentation, phenotype and treatment of patients with IBD and EIBM in our setting.</p><p><b><i>Materials and methods:</i></b> Multicenter descriptive observational multicenter study in adult and pediatric patients with IBD and IMD, in which information was collected on different dates in different institutions of patients diagnosed with IBD, treated as outpatients in different Colombian cities in 2021.</p><p><b><i>Results:</i></b> out of a total of 266 patients, 197 (74.1%) presented MEI, predominantly women (67.5%). 95% were adults, and 5% were pediatric patients. 134/197 (68%) with ulcerative colitis (UC), and (63/197) 32% with Crohn's disease (CD), mostly in remission (82.2%) and requiring biologic therapy in 49.2%. Regarding the type of MEI, joint MEI were the most frequent (36%), followed by skin (34.5%), and ocular (24.9%). The frequency of hospitalization due to disease activity was 72.5%, overall vaccination rate was over 70%, and the frequency of cancer was 1.5%. There was a low rate of complications and hospitalization for COVID-19. In women, pregnancy complications were more frequent in CD.</p><p><b><i>Conclusions:</i></b> The MEI in IBD are frequent in our environment, predominate in women, with CD, with more extensive disease, and greater requirement of biologic therapy. Optimal clinical results were found, such as earlier diagnosis of IBD, low morbimortality, acceptable vaccination rate, access to required therapy, and reduction of complications.</p><p><b>PP-02-073</b></p><p><b>Impact of the inclusion of a nurse in the multidisciplinary team in inflammatory bowel disease</b></p><p><b>Viviana Parra-izquierdo</b> and Ginary Orduz and Silvia Hoyos and Juliep Sarmiento and Luis Bohorquez and Diego Cano and Oscar Pinto and Hernan Duarte and Carlos Cuadros and Johon Garces and Juan Acevedo and Andres Barco and Juan Frias and Cristian Florez</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> the nurse becomes the first contact with the patient, favoring empathy, the delivery of education, specific treatment indications and continuous follow-up. For this reason we want to describe the role played by the head nurse in a certified center of excellence in Inflammatory Bowel Disease.</p><p><b><i>Objective:</i></b> To describe the interventions carried out by the head nurse of the center of excellence in Inflammatory Bowel Disease to improve the quality of life of patients with this condition.</p><p><b><i>Materials and Methods:</i></b> Descriptive cross-sectional study in which the activities carried out by the nurse of the center of excellence since September 2022, date on which the certification was received, are described.</p><p><b><i>Results:</i></b> Within the strategy of the center of excellence, the role of the nurse is fundamental, carrying out educational and care work with accompaniment and follow-up for both patients and their families, with the aim of having an impact on improving their quality of life. Likewise, the nurse of the Center of Excellence is part of the Intestinal Ultrasonography days in order to perform a less invasive follow-up of the disease, being the only center of excellence in the country to perform this technique.</p><p><b><i>Conclusions:</i></b> In our Center of Excellence the nurse has a fundamental role in the creation, execution and follow-up of the educational, research and assistance programs that are developed in patients with this disease.</p><p><b>PP-02-075</b></p><p><b>Edema index as a more sensitive indicator of nutritional status with Crohn’s disease</b></p><p><b>Yu Peng</b>, Yong Li, Ziheng Peng, Duo Xu, Keke Tang and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To confirm whether the edema index could be a nutritional assessment for Crohn's disease (CD) patients.</p><p><b><i>Materials and Methods:</i></b> The study was conducted at Xiangya Hospital Central South University between July 2023 and December 2023, including CD patients and the control group. In both groups, bioelectrical impedance analysis was performed, and the edema index defined as extracelluar water/total body water (ECW/TBW) was calculated. The demographic data and body composition parameters were collected through the medical system. The control group consisted of healthy adults, strictly matched for gender, age, and BMI in a 1:1 case-control manner. Univariate analysis and multivariate analysis were used to compare the differences in nutrition-related indicators between both groups.</p><p><b><i>Results:</i></b> A total of 126 subjects were included in the final cohort with 50% each of CD patients and healthy individuals. There were no significant differences in gender, age, BMI. Compared to the control group, CD patients had significantly lower fat mass (9.26 ± 5.38 vs.16.63 ± 10.67, P = 0.006), bone mass (2.16 ± 0.41 vs. 2.52 ± 0.57, P = 0.019), but a higher level of edema index (41.07 ± 2.38 vs. 38.53 ± 5.62, P = 0.015). Multivariate logistic regression analysis revealed that edema index was an independent factor to malnutrition in CD patients (OR: 1.665, 95% CI: 1.158-2.395, P = 0.006).</p><p><b><i>Conclusion:</i></b> A higher edema index in CD patients demonstrates their worse nutritional status compared to healthy subjects. The edema index may be a sensitive indicator to assess nutritional status in CD patients.</p><p><b>PP-02-076</b></p><p><b>Platelet-to-albumin ratio: a novel indicator for assessing severity and predicting future surgery in Crohn's disease</b></p><p><b>Yu Peng</b>, Duo Xu, Yong Li, Ziheng Peng and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The purpose of this study is to find a reliable indicator for assessing Crohn's disease (CD) severity and predicting the probability of Gastrointestinal (GI) surgery.</p><p><b><i>Materials and Methods:</i></b> In this study, patients with CD who were hospitalized at the Department of Gastroenterology, Xiangya Hospital, Central South University between January 2017 and June 2022 were included. All data were retrieved from the electronic medical record system. Seven integrated indices were calculated to assess CD severity and predict the probability of future GI surgery in patients with CD. Mann-Whitney U test, logistics regression analysis, Cox regression analysis, and receiver operating characteristic (ROC) curve analysis were used. A p-value of less than 0.05 was considered statistically significant.</p><p><b><i>Results:</i></b> A total of 320 patients with CD were included in this study, with 215 having active disease and 105 having moderate-to-severe CD activity. The platelet-to-albumin ratio (PAR) was found to have a significant independent association with CD activity in both univariate and multivariate analyses. The optimal PAR cutoff values of 8.075 and 9.325 could be used to predict CD activity and moderate-to-severe activity, respectively. The following retrospective study on 215 patients revealed that high PAR could predict the probability of future surgery in patients with CD and B2/B3 disease behavior, with 9.325 being the optimal cutoff value.</p><p><b><i>Conclusion:</i></b> PAR could be used to assess CD severity as well as predict the probability of GI surgery in patients with CD and B2/B3 disease behavior.</p><p><b>PP-02-077</b></p><p><b>Bio-naïve patients had higher response rates at weeks 8 with Ustekinumab: retrospective study in China</b></p><p><b>Yu Peng</b> and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Obejectives:</i></b> To evaluate the real-world short-term efficacy of Ustekinumab (UST) in Chinese Crohn’s disease (CD) patients after its recent approval in 2020.</p><p><b><i>Materials and Methods:</i></b> Retrospective analysis of data from resident patients diagnosed with CD at Xiangya Hospital from July 2020 to May 2023 was performed. The inclusion criteria were moderate-to-severe CD, diagnosed according to the Chinese diagnosis consensus in inflammatory bowel disease, and treatment with UST. Clinical data were collected from the patients’ medical records.</p><p><b><i>Results:</i></b> 112 patients analyzed in the study, including 87 males (77.7%) and 25 females (22.3%), the mean age was 30.7±4.7 years. The most frequent disease type(location) was ileocolonic disease (L3, 63.7%). The most frequent disease behavior was stricturing non-penetrating type according to the Montreal Classification (B2, 47.5%). Most patients (58%) had not received biologics therapy. The clinical response rates at weeks 8 was 69%. Regarding the history of biologics therapy, the response rate was significantly higher in bio-naïve patients than bio-failure patients at week 8 (82.1% vs. 56.7%, p<0.05). The clinical response rates at weeks 8 were not correlated to the patient's age, sex, disease location, disease behavior, presence of perianal disease, CDAI, blood tests, and was correlated to the history of biologics therapy. There was no significant difference in the baseline clinical characteristics between the bio-naïve and bio-failure groups.</p><p><b><i>Conclusion:</i></b> Bio-naïve patients had higher response rates at weeks 8 with UST which support the short-term effectiveness of UST in Chinese CD patients in real-world practice.</p><p><b>PP-02-078</b></p><p><b>Impact of Crohn’s disease on working life: Discovering the truth in Chinese patients</b></p><p><b>Yu Peng</b>, Yong Li, Ziheng Peng, Duo Xu, Keke Tang and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To obtain an eastern perspective of the impact of Crohn's disease (CD) on patients’ working lives.</p><p><b><i>Materials and Methods:</i></b> A cross-sectional survey was performed on patients with CD who were hospitalized at Xiangya Hospital of Central South University from January to October in 2023. Data were analyzed using Chi-squared test, Logistic regression.</p><p><b><i>Results:</i></b> A total of 356 patients responded the validated questionnaire and all of them met the requirements and were used for analysis. Among the respondents, 273 (76.7%) male and 83(23.3%) female. The career change was related to the following factors: 1) educational level (p < 0.05);2) income of patients after onset (p<0.05); 3) c. the changing of occupational promotion after onset (p < 0.001);4) the impact of finding a new career (p < 0.005); 5)occupational or job discrimination (p < 0.005). Other variables have no significant correlation with this outcome. Based on multivariate logistic regression, the independent risk factors for career change in Chinese Crohn's disease patients were income of patients after onset (odds ratio (OR): 6.226, 95% confidence interval (CI): 0.090-0.749, p=0.013), and the changing of occupational promotion after onset (OR: 7.069 95% CI: 0.105-0.711, p=0.008).</p><p><b><i>Conclusion:</i></b> This is one of the few surveys on the impact of CD on professional life of chinese patients. Overall, CD had a strong negative impact on professional life. CD affects career changes, it restricts the access to occupational promotion after onset, and further influence on family life. This issue should be systematically investigated to develop adapted measures.</p><p><b>PP-02-079</b></p><p><b>Diagnosis and management colitis tuberculosis in severe malnutrition patient</b></p><p><b>Sabilla Laras Permana</b> and Deka Larasati</p><p><i>Department of Internal Medicine, Gatot Soebroto Army Central Hospital, Jakarta Pusat, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Tuberculosis (TB) can present in various forms, including colitis tuberculosis, which may lead to severe malnutrition. Malnutrition is associated with a twofold higher risk of dying in patients with tuberculosis (TB) and considered an important potentially reversible risk factor for failure of TB treatment.</p><p><b><i>Case Report:</i></b> A 20-year-old woman presented with intermittent abdominal pain, diarrhea with blood, a 20 kg weight loss. She had no reported medical history, her sister had a TB pulmonal and had been treated. Initial labs were notable for severe anemia (Hb 6.8), albumin (1.1), and positive Stool GeneXpert assay. Imaging studies showed bilateral pleural effusion and abdominal CT findings suggestive of colitis tuberculosa with associated lymphadenitis. Colonoscopy showed multiple ulcerations in the cecum and ileum, Biopsies confirmed chronic ulcerative colitis with active inflammation TB-PCR assay of the biopsies was positive. Soon after antituberculin therapy was initiated, and alongside nutritional support the patient's condition improved significantly, with resolution of gastrointestinal symptoms and gradual weight gain.</p><p><b><i>Discussion:</i></b> This case highlights the importance of recognizing colitis tuberculosis as a potential diagnosis in young patients presenting with gastrointestinal issues, particularly in the context of a family history of tuberculosis. Early diagnosis and appropriate management are crucial to improve outcomes and prevent severe complications. Optimizing nutrition has demonstrated beneficial effects on prognosis and the response to drug therapy for patients with TB, although the evidence is limited and heterogeneous.</p><p><b>PP-02-080</b></p><p><b>Tuberculosis in patients with inflammatory bowel disease: a case series from Singapore</b></p><p><b>Tian Yu Qiu</b> and Jeannie Peng Lan Ong and Tiing Leong Ang and Lai Mun Wang and Chin Kimg Tan</p><p><i>Singhealth, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/objective:</i></b> Tuberculosis (TB) pose significant health concern among patients with Inflammatory Bowel Disease (IBD) especially those on anti-tumour necrosis factor (anti-TNF). This case series aim to describe the varied presentations of TB in IBD patients in Singapore, a TB endemic country.</p><p><b><i>Methods:</i></b> This is a case series of 4 IBD patients on follow-up with Changi General Hospital (Singapore) and diagnosed with TB in 2023. Demographics, IBD treatment, TB diagnosis and subsequent treatment were collected (Table 1).</p><p><b><i>Results:</i></b> Patient 1 had Crohn’s Disease (CD) on Adalimumab. TB quantiferon was negative pre- treatment but he developed disseminated TB after 12 months of anti-TNF.</p><p>Patient 2 was on Adalimumab for IBD-unclassified. He had latent TB infection despite initial negative TB quantiferon.</p><p>Patient 3 had Ulcerative Colitis (UC) on Azathioprine. He was in endoscopic remission when diagnosed with pulmonary TB. He stopped Azathioprine and developed Acute Severe Ulcerative Colitis (ASUC) 2 month later.</p><p>Patient 4 was initially diagnosed as ileal CD with endoscopy findings of terminal ileal (TI) ulcer. She received 8-weeks of Budesonide. Repeat colonoscopy for persistent symptoms showed persistent TI ulcer, acid-fast bacilli on Ziehl-Neelsen stain confirmed TB ileitis.</p><p>First two cases highlight the risk of TB reactivation on anti-TNF despite initial negative screening. TB gut may also mimic IBD endoscopically. It is crucial to repeat endoscopy for IBD patients who are not responding to initial therapy.</p><p><b>PP-02-081</b></p><p><b>Mixed-Strain Probiotics Reduce Colitis Severity in DNBS-Induced Colitis Rats</b></p><p><b>Pakkapon Rattanachaisit</b>, Nathawadee Lerttanatum, Somying Tumwasorn, Natthaya Chuaypen, Thasinas Dissayabutra and Duangporn Werawatganon</p><p><i>Chulalongkorn University, Bangkok, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Inflammatory bowel disease (IBD) is characterized by chronic, relapsing inflammation influenced by genetic, microbial, and environmental factors. This study aims to determine the effectiveness of multi-strain probiotics in reducing colitis severity in rats, assessing clinical responses, histopathology, and inflammatory mediators.</p><p><b><i>Materials and Methods:</i></b> Forty male Sprague-Dawley rats, aged six weeks, were used and divided into four groups: Control, Acute Colitis (AC), RP (treated with Lactobacillus rhamnosus and Lactobacillus paracasei), and SG (treated with Lactobacillus salivarius and Lactobacillus gasseri). Probiotics were administered via gavage daily, starting three days before acute colitis induction with rectal DNBS solution and continuing for seven days. On day eight, rats were euthanized for colon and blood sample collection for further evaluation.</p><p><b><i>Results:</i></b> The AC group exhibited significantly elevated TNF-α levels compared to Control, RP, and SG groups (p < 0.001). RP and SG treatments significantly reduced TNF-α levels compared to AC (p = 0.002, p < 0.001, respectively). Trends indicated increased IL-10 levels and decreased MDA levels in RP and SG groups, though not statistically significant (Table 1). Histopathological analysis showed a lower median Ameho’s score in the RP group compared to AC, without statistical significance.</p><p><b><i>Conclusion:</i></b> Multi-strain probiotics significantly reduced TNF-α levels, suggesting an anti-inflammatory effect in colitis. Trends in IL-10 and MDA levels and histopathological improvements, though not statistically significant, indicate potential benefits. Further research with larger sample sizes is needed to confirm these findings and explore probiotics as a therapeutic option for IBD.</p><p><b>PP-02-082</b></p><p><b>Changes in trimethylamine-N-oxide levels in ulcerative colitis: relationship with clinical and laboratory indicators</b></p><p><b>Доктор Nadezhda Samoilova-Bedych</b> and Yelena Laryushina and Lyudmila Turgunova and Alexander Marchenko and Adina Igenbekova</p><p><i>NCJSC \"Karaganda Medical University\", Karaganda, Kazakhstan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> to compare the level of TMAO in healthy individuals and patients with ulcerative colitis and to consider the possibility of using TMAO as a biomarker in the diagnosis of ulcerative colitis based on its changes on different clinical, laboratory, and endoscopic activity of the disease.</p><p><b><i>Material and methods:</i></b> the study included 63 patients with ulcerative colitis and 38 healthy individuals, who were measured TMAO levels by high-performance liquid chromatography with mass spectrometry.</p><p><b><i>Results:</i></b> the results showed a statistically significant decrease in TMAO levels in patients with ulcerative colitis compared to the healthy group (p<0.0001), TMAO differed in clinically active and inactive disease (p=0.003). Additional analysis showed a correlation between TMAO levels and clinical-laboratory parameters- age (r=0.377, p=0.003), stool frequency (r=-0.427, p=0.001), WBC (r=-0.31, p=0.042), serum albumin (r=0.379, p=0.002) and fecal calprotectin (r=-0.314, p=0.022): TMAO significantly correlated with disease activity according to the Montreal scale (r=-0.389, p=0.002) and severity of attack according to Truelove-Witts (r=-0.301, p=0.027).</p><p><b><i>Conclusion:</i></b> the results emphasize the potential role of TMAO as a biomarker for diagnosis and assessment of ulcerative colitis activity. Further studies are needed to confirm these observations and develop standardized protocols for using TMAO in clinical practice.</p><p><b>PP-02-083</b></p><p><b>Changes in the expression of lipopolysaccharide-binding protein in patients with ulcerative colitis and Crohn's disease.</b></p><p><b>Доктор Nadezhda Samoilova-Bedych</b> and Yelena Laryushina and Lyudmila Turgunova and Alexander Marchenko and Aliya Zhirenbayeva</p><p><i>NCJSC \"Karaganda Medical University\", Karaganda, Kazakhstan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> the study aims to investigate changes in the expression of lipopolysaccharide-binding protein (LPB-protein) in patients with ulcerative colitis (UC) and Crohn's disease (CD) at different disease activities.</p><p><b><i>Material and methods:</i></b> an observational cross-sectional study was conducted at the University Hospital of NCJSC \"KMU\". 112 patients with IBD (63 with ulcerative colitis and 48 with Crohn's disease) and 41 healthy individuals were analyzed for LPS-protein concentration by enzyme-linked immunosorbent assay.</p><p><b><i>Results:</i></b> differences in LPB-protein expression levels were obtained in patients with UC and CD compared to the control group (p=0,05). At the same time, the median in the group with UC (Me 16.8 ng/mL) was higher than in patients with CD (Me 14.2 ng/mL). LPB-protein index had no statistically significant changes depending on the lesion extent, clinical and laboratory activity according to Trulove-Witts and CDAI scales, Meyo and Harvey-Bradshaw indices, endoscopic activity in UC and CD.</p><p><b><i>Conclusions:</i></b> our data indicate the presence of changes in the expression level of LPS-binding protein in patients with UC and CD compared to healthy individuals. This suggests the presence of changes in the microbiome in patients with IBD in favor of a predominance of Gram-negative flora with greater expression in UC. However, for more in-depth study of LPB-protein changes at the different activity of UC and CD, additional studies are needed.</p><p><b>PP-02-084</b></p><p><b>Inhibition of IL-17 signaling induces intestinal mucosal inflammation</b></p><p><b>Yosuke Shimodaira</b>, Sho Fukuda, So Takahashi and Katsunori Iijima</p><p><i>Akita University Graduate School Of Medicine, Akita City, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> IL-17 is a key cytokine in various inflammatory disease. Targeting IL-17 signals blockage did not improve inflammatory bowel disease (IBD) clinically, but also even developed de novo enteritis in non-IBD subjects. Little is known about the effects of blocking IL-17 signaling on intestinal homeostasis. This study investigated intestinal immunity under the inhibition of IL-17 signaling.</p><p><b><i>Methods:</i></b> Refractory patients with psoriasis who started receiving anti-IL-17 or anti-IL-17 receptor inhibitors were included in this study. Evaluation for fecal immunochemical test (FIT), fecal calprotectin (fCal), endoscopic findings, colonic mucosa histology, and fecal microbial composition before and 3 months after starting treatment on the subjects was performed. Fecal microbial composition was analyzed with 16S rRNA.</p><p><b><i>Results:</i></b> Five psoriasis patients received antibodies. The median FIT level and fCal level was 0 ng/mL (SD 162) and 39.4 mg/kg (SD 180) at baseline. Thereafter, the median FIT level and fCal level on 3 months after initiating antibody therapy increased to 19 ng/mL (SD 136) and 222 mg/kg (SD 141), respectively. Endoscopic findings before administration showed mild edema in one patient and mild redness in one patient, and thereafter mild edema in two patients and mild redness in two patients after administration. The number of mononuclear cells infiltrating in the mucosa significantly increased with antibody administration. Microbial community analysis showed alteration in alpha diversity after antibody administration. Proteobacteria was increased after the administration.</p><p><b><i>Conclusions:</i></b> Blocking IL-17 signals altered intestinal homeostasis toward mucosal inflammation.</p><p><b>PP-02-085</b></p><p><b>Association of Fecal Calprotectin with Ulcerative Colitis Disease Activity and Severity.</b></p><p><b>Marsela Sina</b>, Afërdita Djegsi, Sara Hoxha, Xhensila Pemaj and Skerdi Prifti</p><p><i>University Clinic of Gastrohepatology, Dept of Internal Diseases, University of Medicine, Tirana, Albania</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aim to evaluate the association between fecal calprotectin (FC) and Ulcerative Colitis (UC) disease severity.</p><p><b><i>Materials and Methods:</i></b> 82 UC patients underwent colonoscopy, from January 2018 to June 2024 at the University Clinic of Gastrohepatology. Laboratory data, clinical and endoscopic scores were used to assess the disease activity.</p><p>The data were analyzed by independent test, correlation test and receiver operating characteristic (ROC) curve analysis. A p<0.05 was considered statistically significant.</p><p><b><i>Results:</i></b> 38 (46.3%) male and 44 (53.7%) female patients were included in the study. Mean age was 42.46 ± 13.2 years. 2 (2.4%) patients had ulcerative proctitis; 44 (53.7%) left-sided colitis, 36 (43.9%) patients had pancolitis. Mean disease duration was 6.63 ± 6.76 years. 30.5% of patients were in remission, while 69.5% had active disease.</p><p>We found a significant positive correlation between FC and partial Mayo Score (r=0.364, p=0.001); FC and endoscopic Mayo score (r=0.338, p=0.002), FC and total Mayo score (r=0.374, p=0.001); and FC and Ulcerative Colitis Endoscopic Index of Severity (UCEIS) Score (r= 0.368 p=0.001). A significant association was found between FC value and severity of disease (p= 0.008). In multiple regression analysis, FC significantly statistically correlates only with UCEIS Score (p=0.015).</p><p>The optimum cut-off values of FC reflecting active UC was 162.18ug/g with 83.6% sensitivity and 39.1% specificity. (AUC 0.825, 95%CI: 0.717-0.934).</p><p><b><i>Conclusion:</i></b> Our study showed strong correlations between FC levels and disease severity scores in UC. A FC cut-off of 162.18μg/g, was 83.6% sensitive for detecting active disease, with an AUC of 0.825.</p><p><b>PP-02-086</b></p><p><b>Risk factors of low bone density in inflammatory bowel disease patients</b></p><p><b>Marsela Sina</b>, Sara Hoxha, Afërdita Djegsi and Adriana Babameto and Skerdi Prifti</p><p><i>University Clinic of Gastrohepatology, Dept of Internal Diseases, University of Medicine., Tirana, Albania</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate possible risk factors of low bone density among our Inflammatory Bowel Disease (IBD) patients.</p><p><b><i>Materials and Methods:</i></b> This is a prospective study conducted at the University Hospital Center Mother Teresa, on IBD patients who underwent Bone Mineral Density (BMD) assessments with dual-energy X-ray absorptiometry scans from January to December 2023. Demographic, laboratory data, disease duration, activity and treatment were collected for each patient.</p><p><b><i>Results:</i></b> 36 IBD patients, mean age 45.86 ± 15 years; 33.3% males; 66.7% females. 16.7% Crohn’s Disease and 83.3% Ulcerative Colitis patients were included in the study. 91.7% were non-smokers; 33.3% on corticosteroid therapy; 66.6% on biologic treatment; 16.6% were taking vitamin D, and 8.3% patients underwent ileo-colic resection.</p><p>The prevalence of osteopenia and osteoporosis was 38.9% and 27.8% respectively. 36.1% of cases had osteopenia at the lumbar spine, 33.3% of cases at the femoral neck. 22.2% of cases had osteoporosis at the lumbar spine and 5.6% of cases at the femoral neck.</p><p>A strong positive correlation was found between low lumbar T score and age (r=0.618, p<0.01), female gender (r=0.362, p=0.034) and steroid treatment (r=0.408, p=0.014). A strong positive correlation was found between low femoral T-score and age (r=0.459, p=0.005) and disease duration (r=0.347, p=0.038). A strong negative correlation was found between biological treatment and low lumbar T score (r=-0.351, p=0.036). No correlation was found between low BMD and type of IBD disease.</p><p><b><i>Conclusion:</i></b> In our patients, low BMD is correlated to older age, female gender, disease duration and corticosteroid therapy.</p><p><b>PP-02-087</b></p><p><b>Which inflammatory biomarkers are better predictors of UC activity? Our Experience</b></p><p><b>Marsela Sina</b>, Afërdita Djegsi, Sara Hoxha, Xhensila Pemaj and Skerdi Prifti</p><p><i>University Clinic Of Gastrohepatology, Dept. of Internal Disease, University of Medicine, Tirana, Albania</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In clinical practice, there are several biomarkers used to monitor ulcerative colitis (UC) disease activity. We aim to evaluate which inflammatory biomarkers are better to predict disease activity in (UC) patients.</p><p><b><i>Materials and Methods:</i></b> This is a prospective study of 82 cohort UC patients who underwent endoscopic evaluation, from January 2018 to June 2024 at the University Clinic of Gastrohepatology, University Hospital Center Mother Teresa, Tirana. Demographics, laboratory data and Ulcerative Colitis Endoscopic Index of Severity (UCEIS) score was collected for each patient. UC was considered active when UCEIS >2 points.</p><p><b><i>Results:</i></b> 38 (46.3%) male and 44 (53.7%) female patients were included with a mean age of 42.46 ± 13.2 years. 2 (2.4%) patients had ulcerative proctitis; 44 (53.7%) patients had left-sided colitis and 36 (43.9%) patients had pancolitis. 25 (31%) patients were in remission 57 (69%) had active disease. Mean disease duration was 6.63 ± 6.76 years.</p><p>We found a significant positive association of fecal calprotectin (FC) (r=0.368, p=0.001); WBC (r=0.457, p<0.001); neutrophil/lymphocyte ratio (r=0.418, p=0.001); CRP (r=0.396, p=0.001); CRP/albumin (r=0.436, p=0.002), and ferritin (r=0.250, p=0.039) with UCEIS score. A significant negative association was found between UCEIS score and lymphocyte/monocyte ratio (r=-0.324, p= 0.004) and albumin (r=-0.486, p=0.001).</p><p>In multiple linear regression analysis significant predictors included FC (β=0.687, p = 0.015), CRP (β = 0.416, p = 0.011), and the CRP/Albumin ratio (β = -4.491, p = 0.015).</p><p><b><i>Conclusion:</i></b> These findings demonstrate that FC, CRP and CRP/Albumin are important inflammatory biomarkers in order to predict UC activity.</p><p><b>PP-02-088</b></p><p><b>The simple non-invasive markers as the potential markers in inflammatory bowel disease</b></p><p><b>Yohanna Fransisca Sinuhaji</b><sup>1</sup>, Ilhamd Ilhamd<sup>2</sup>, Masrul Lubis<sup>2</sup>, Imelda Rey<sup>2</sup> and Taufik Sungkar<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Inflammatory bowel disease (IBD) is caused by multifactorial, one factor is an abnormal immune response to gut microflora. The neutrophil-to-albumin ratio (NAR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), erythrocyte sedimentation rate (ESR), are the simple non-invasive systemic inflammatory markers in various medical condition, including inflammation disease, blood vessel disorder and cancer.</p><p><b><i>Objective:</i></b> To evaluate the simple non-invasive markers as diagnostic potential marker in IBD patients.</p><p><b><i>Method:</i></b> This was case control study using electronic medical record at H. Adam Malik Hospital. A total of 36 samples of IBD and healthy population as control group were included in the study. Clinical characteristics, NAR, NLR, PLR, ESR were collected.</p><p><b><i>Results:</i></b> The most clinical manifestations were stomachache and bloody stools. IBD patients suffered anemia, hypoalbuminemia, as well significant differences in NLR, PLR, ESR compared to with control with p value < 0.001. There was significant differences between NAR, NLR, PLR, ESR in the IBD group with control. The receiver-operating characteristic (ROC) analysis revealed the optimal cutoff of NAR to predict IBD was 13,89, with sensitivity and specificity of 88.9% and 88.9%, respectively. For NLR, the best cut-off value was 2.33, with sensitivity and specificity of 83.3% and 83.3%, respectively. For PLR, the best cut-off value was 140.573, with sensitivity and specificity of 83.3% and 83.3%, respectively. For ESR, the best cut-off value was 23.5, with sensitivity and specificity of 94.4% and 94.4%, respectively.</p><p><b><i>Conclusion:</i></b> NAR, NLR, PLR, ESR as simple non-invasive markers could be used to predict diagnosis of Inflammatory Bowel Disease.</p><p><b>PP-02-089</b></p><p><b>Inflammatory bowel disease is associated with attenuated hepatic steatosis by Fibroscan</b></p><p><b>Yeo Won Sohn</b><sup>1</sup>, Kwang Woo Kim<sup>1</sup>, Bo Kyung Kim<sup>1</sup>, Hee Jun Jang<sup>1</sup>, Dong Kee Jang<sup>1</sup>, Seong Joon Koh<sup>2</sup>, Su Hwan Kim<sup>1</sup>, Hyoun Woo Kang<sup>1</sup>, Yong Jin Jeong<sup>1</sup>, Byeong Gwan Kim<sup>2</sup>, Kook Lae Lee<sup>1</sup> and Ji Won Kim<sup>1</sup></p><p><sup>1</sup><i>Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, South Korea;</i> <sup>2</sup><i>Seoul National University Medical Center, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study is designed to find out relationship between IBD and either hepatic steatosis or liver fibrosis scanned by Fibroscan.</p><p><b><i>Materials and Methods:</i></b> This is a prospectively enrolled, cross-sectional and single-center observational study. Subjects with inflammatory bowel disease who have been treated between June 2018 and April 2024 were enrolled. Subjects planned for health screening during the same period were matched as the control group. Hepatic steatosis and fibrosis were evaluated with controlled attenuation parameter (CAP) and liver stiffness measurement (LSM) by Fibroscan, respectively.</p><p><b><i>Results:</i></b> 279 subjects were enrolled. 142 and 137 subjects were in the group of IBD and control, respectively. IBD group showed significantly less CAP score than that of the control group. (215.8 ± 54.2 vs. 247.9 ± 51.9, p < 0.001) However, there was no significant difference in LSM score between the IBD and control group. (4.1 ± 1.4 vs. 4.7 ± 6.2, p = 0.312) Both ulcerative colitis (UC) and Crohn’s disease (CD) group showed consistent tendency when compared with the control group. (247.9 ± 51.9 vs 220.4 ± 54.4, p < 0.001; 247.9 ± 51.9 vs 205.3 ± 52.8, p < 0.001, respectively) The multivariable analysis showed that IBD was still inversely associated with hepatic steatosis (adjusted odds ratio 0.506, 95% confidence interval 0.293 - 0.876, p = 0.015).</p><p><b>PP-02-090</b></p><p><b>CT/MR Enterography: Utilization in Filipino patients with Inflammatory Bowel Disease</b></p><p><b>Jose Luis Matthias Sollano</b></p><p><i>Makati Medical Center, Makati, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Methods:</i></b> Researchers retrospectively analyzed data over 10 years from patients with suspected or diagnosed IBD who underwent MRE or CTE. Patients with known GI tuberculosis, colorectal cancer, or prior abdominal surgeries were excluded. MRE and CTE findings were suggestive of IBD were recorded and compared.</p><p><b><i>Results:</i></b> Fifty patients were included, with 32 undergoing MRE and the rest having CTE. MRE and CTE detected additional small bowel lesions in 90.9% of patients with known CD. Common findings included wall thickening and pericolic fat stranding. Similarly, in 93.3% of suspected IBD cases, MRE and CTE identified small bowel involvement compatible with CD.</p><p><b><i>Conclusion:</i></b> This study highlights the real-world use of MRE and CTE in managing IBD, particularly CD, in the Philippines. The additional information obtained from these procedures aids in accurate diagnosis and comprehensive management of Filipino IBD patients.</p><p><b>PP-02-091</b></p><p><b>Comparison of Ustekinumab and Vedolizumab for Moderate-to-Severe UC with Prior Failure of Biologics or SMDs</b></p><p><b>Seung Min Hong</b><sup>1</sup>, Dong Hoon Baek<sup>1</sup>, Geun Am Song<sup>1</sup>, Dong Chan Joo<sup>1</sup>, Tae In Kim<sup>1</sup>, Gwang Ha Kim<sup>1</sup>, Bong Eun Lee<sup>1</sup>, Cheolung Kim<sup>1</sup> and Hyeon Tae Cho<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea;</i> <sup>2</sup><i>Centum Medihill Hospital, Busan, Republic of Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study compared the effectiveness of Ustekinumab (UST) and vedolizumab (VDZ) in patients with moderate to severe ulcerative colitis who have previously failed treatment with biologics or small molecule drugs (SMDs).</p><p><b><i>Materials and Methods:</i></b> The study retrospectively analyzed the medical records of a total of 43 patients with moderate to severe ulcerative colitis, comprising 15 patients in the UST group and 28 in the VDZ group. All of these patients had previously experienced failure with treatments involving biologics or SMDs.</p><p><b><i>Results:</i></b> At week 52 following the initiation of UST or VDZ treatment, there were no statistically significant differences between the two groups in terms of the clinical remission rate (UST group 60.0% vs. VDZ group 67.9%, p = 0.606) and the corticosteroid-free remission rate (UST group 46.7% vs. VDZ group 57.1%, p = 0.512). At the time of response evaluation following the induction phase, the proportion of patients showing an endoscopic response did not demonstrate a statistically significant difference between the two groups (UST group 80.0% vs. VDZ group 78.6%, p = 1.000). However, at week 52, the drug survival rate was significantly higher in the UST group at 93.3% compared to 71.4% in the VDZ group (p = 0.031). There were no significant differences in adverse events between the two groups.</p><p><b><i>Conclusion:</i></b> In patients with moderate to severe ulcerative colitis who had previously failed treatment with biologics or small SMDs, UST demonstrated a superior drug survival rate compared to VDZ.</p><p><b>PP-02-092</b></p><p><b>Usefulness of Underwater EMR for neoplastic lesions in ulcerative colitis</b></p><p><b>Kaoru Takabayashi</b><sup>1</sup>, Yuri Imura<sup>1</sup>, Shoma Murata<sup>1</sup>, Daisuke Minezaki<sup>3</sup>, Anna Tojo<sup>1</sup>, Hinako Sakurai<sup>1</sup>, Kentaro Iwata<sup>3</sup>, Kurato Miyazaki<sup>3</sup>, Teppei Masunaga<sup>3</sup>, Mari Mizutani<sup>1</sup>, Teppei Akimioto<sup>3</sup>, Yusaku Takatori<sup>3</sup>, Yusuke Yoshimatsu<sup>2</sup>, Shinya Sugimoto<sup>2</sup>, Hiroki Kiyohara<sup>2</sup>, Shintaro Kawasaki<sup>1</sup>, Yohei Mikami<sup>2</sup>, Noriko Matsuura<sup>3</sup>, Tomohisa Sujino<sup>1</sup>, Atsushi Nakayama<sup>3</sup>, Naohisa Yahagi<sup>3</sup>, Motohiko Kato<sup>1</sup> and Takanori Kanai<sup>2</sup></p><p><sup>1</sup><i>Center For Diagnostic And Therapeutic Endoscopy, Keio University School Of Medicine, Tokyo, Japan;</i> <sup>2</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;</i> <sup>3</sup><i>Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> According to Western guidelines, endoscopic resection (ER) is acceptable for neoplastic lesions with clear boundaries, even within areas affected by ulcerative colitis (UC), however, this procedure is also difficult due to advanced fibrosis of mucosa associated with previous inflammation within areas affected by UC. Underwater endoscopic mucosal resection (UEMR)has become popular in recent years and has been useful for lesions with advanced fibrosis. In this study, we verified the usefulness of UEMR for lesions diagnosed as sporadic neoplasms (SN) before ER, among neoplastic lesions that developed within areas affected by UC.</p><p><b><i>Material and Methods:</i></b> We enrolled 35 lesions in 26 patients that underwent UEMR for neoplastic lesions 20 mm or smaller in diameter, which had developed within areas affected by UC, from December 2021 to February 2023 at Keio University Hospital. The primary endpoint was R0 resection rate, while the secondary endpoints were en bloc resection rate, procedure time. and adverse events.</p><p><b><i>Results:</i></b> Background mucosa consisted of normal mucosa (regenerated mucosa after inflammation) in nine lesions (25.7%), atrophic mucosa in 20 lesions (57.1%), and atrophic mucosa with ulcer scars in six lesions (17.2%). A total of 34 lesions (97.1%) underwent en bloc resection; of them, 29 lesions (85.3%) underwent R0 resection, while only one (2.9%) underwent piecemeal resection. In terms of procedural accidents, there were no cases of intraoperative perforation, delayed perforation, post polypectomy coagulation syndrome, and delayed bleeding.</p><p><b><i>Conclusion:</i></b> UEMR may be an effective and safe endoscopic resection method for SN within areas affected by UC.</p><p><b>PP-02-093</b></p><p><b>Risk factors and response predictors of iron deficiency without anemia in patients with IBD</b></p><p><b>Sunil Thomas George</b>, Rajesh Gopalakrishna, Priya Nair, Sharon Paul Methala, Anoop K Koshy and Shine Sadasivan</p><p><i>Amrita Institute of Medical Sciences, Kochi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To determine the prevalence of iron deficiency without anemia (IDWA) in patients with inflammatory bowel disease (IBD) and to evaluate the effects of iron supplementation.</p><p><b><i>Materials and Methods:</i></b> Our prospective interventional non-randomized study was conducted over 12 months in 94 consecutive patients with confirmed IBD. Clinical and biochemical parameters including complete blood count, serum ferritin, serum iron, transferrin saturation (TFS) and C-reactive protein, were recorded. Health-related quality of life (HRQoL) was assessed using IBDQ-9 and FACIT-F questionnaires. Iron deficiency was diagnosed on basis of serum ferritin levels (<100 μg/L with active inflammation or <30 μg/L without inflammation) and TFS <16%. Iron supplementation was administered; and parameters were reassessed at one and three months. Statistical analyses included Chi-square tests, multivariate binary logistic regression, and paired sample t-tests.</p><p><b><i>Results:</i></b> We found that 55(58.6%) of IBD patients were iron deficient. The iron-deficient group had a higher proportion of women (59%) compared to men (41%). Among the iron-deficient group, the baseline mean hemoglobin level was 12 g/dL which improved to mean of 14 g/dL by the third month following iron supplementation. The hemoglobin levels in the normal group remained stable throughout the study period. Iron deficiency was seen to have a significant impact on quality of life.</p><p><b><i>Conclusion:</i></b> Iron deficiency without anemia is a common and clinically significant condition in IBD patients, adversely affecting their quality of life. Screening for iron deficiency and timely iron supplementation leads to significant improvements in hemoglobin levels and enhances overall quality of life.</p><p><b>PP-02-094</b></p><p><b>Macrophage-Derived Asparagine Promotes Fibroblast Proliferation and Drives Intestinal Fibrosis</b></p><p><b>Yiwen Tu</b>, Haiming Zhuang, Yubei Gu, Yao Zhang and Duowu Zou</p><p><i>Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School Of Medicine, Shanghai, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study investigates the reprogramming of amino acid metabolism in intestinal fibrosis, focusing on the metabolic interaction between macrophages and fibroblasts via asparagine.</p><p><b><i>Materials and Methods:</i></b> Metabolomics were used to measure metabolite levels in normal and fibrotic intestinal tissues from Crohn's disease patients (n=122). Metabolic tracing using C13- and N15-labeled asparagine and aspartate was conducted in mice and human primary cells both in vitro and in vivo. Co-culture experiments of primary macrophages and fibroblasts were performed. Gene expression was investigated using qRT-PCR, Western Blot, and immunofluorescence. Col1a2Cre ASRGL1flox mouse model was constructed to explore the potential of targeting asparaginase in treating intestinal fibrosis.</p><p><b><i>Results:</i></b> Crohn's disease patients showed notable metabolic differences between normal and fibrotic tissues, with amino acid metabolism being the most affected. Amino acids were generally downregulated in fibrotic areas, except for a significant increase in aspartate (p<0.01). Both synthesis and degradation of asparagine were markedly elevated in fibrosis (p<0.05). Single-cell transcriptomics suggested that asparagine synthetase (ASNS) in macrophages and asparaginase (ASRGL1) in fibroblasts were upregulated during fibrosis. This was confirmed by in situ immunofluorescence and qPCR. In vitro, TGF-β enhanced asparagine production in macrophages, and ASNS knockout reduced fibroblast proliferation. C13-labeled asparagine tracing showed fibroblasts use it for nucleotide synthesis. Conditional ASRGL1 knockout significantly reduced fibroblasts proliferation and alleviated intestinal fibrosis.</p><p><b><i>Conclusion:</i></b> In intestinal fibrosis, fibroblasts uptake excess asparagine synthesized by macrophages and utilize asparaginase to convert it into aspartate for nucleotide synthesis, thereby promoting their proliferation. Targeting asparagine catabolism could alleviate intestinal fibrosis in mice.</p><p><b>PP-02-095</b></p><p><b>AXL-dependent autophagy impairment differentiates monocyte-derived macrophages from Crohn’s disease and intestinal tuberculosis.</b></p><p><b>Sonakshi Udinia</b> and Mrinmoy Das and Shaina Jamwal and Manasvini Markandey and Aditya Bajaj and Lalita Mehra and Saurabh Kedia and Carey F. H. Lim and Shihui Foo and Prasenjit Das and Shanshan Howland and Mrutyunjay Suar and Amit Singhal and Vineet Ahuja and Dhiraj Kumar</p><p><i>International Centre For Genetic Engineering And Biotechnology, New Delhi, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Macrophages are critical to maintain intestinal homeostasis and contribute to localized inflammation once dysregulated. Here, we aimed to understand innate macrophage dysfunction in the subjects with chronic idiopathic gut inflammation [Crohn’s Disease (CD)] and chronic infection-related gut inflammation [Intestinal Tuberculosis (ITB)].</p><p><b><i>Materials and Methods:</i></b> RNA-seq analysis of ex vivo Mtb-infected or uninfected monocyte-derived macrophages (MDMs) from 8 CD, 5 ITB and 5 controls was done. Subsequently, MDMs from a cohort of patients, including CD (n=42) and ITB (n=41) were characterised for autophagy (CD, n=15: ITB, n=09), mitochondrial depolarization (CD, n=08: ITB, n=08), and bactericidal capacity (CD, n=25: ITB, n=28), at basal state or in response to ex vivo Mtb infection. Finally, the role of Axl downregulation in impaired autophagy was tested.</p><p><b><i>Results:</i></b> RNA-seq analysis of MDMs from CD and ITB subjects revealed differential regulation of autophagy-associated pathway genes either basally or upon ex vivo Mtb infection. CD MDMs also showed significantly impaired autophagy compared to ITB MDMs. MDMs from CD and ITB subjects were permissive to Mtb uptake; however, CD MDMs allowed relatively better survival. We noted that AXL was consistently downregulated in CD MDMs compared to ITB, which was also reflected in the biopsies. AXL knockdown in Thp-1 macrophages impaired autophagy flux, suggesting a causal relationship between reduced AXL expression and impaired autophagy in CD MDMs.</p><p><b><i>Conclusion:</i></b> Autophagy impairment can explain the heightened chronic inflammation observed in CD subjects. Identifying signatures associated with autophagy impairment could facilitate easy diagnostic and novel intervention approaches against CD.</p><p><b>PP-02-096</b></p><p><b>Clinical Characteristics and Treatment Outcomes of Isolated Perianal Crohn's Disease</b></p><p><b>Wasuwit Wanchaitanawong</b><sup>1,2</sup>, Marianee Salaemae<sup>2</sup>, Varut Lohsiriwat<sup>3</sup> and Julajak Limsrivilai<sup>2</sup></p><p><sup>1</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;</i> <sup>2</sup><i>Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand;</i> <sup>3</sup><i>Division of Colon and Rectal Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To compare characteristics among patients with isolated perianal Crohn's disease (ipCD), perianal Crohn's disease with luminal disease (pCD), and recurrent cryptoglandular disease (CGD), and to compare treatment outcomes between ipCD and pCD patients.</p><p><b><i>Materials and Methods:</i></b> We performed a retrospective cohort study of consecutive patients with complex perianal fistula (CPF) confirmed by pelvic MRI at Siriraj Hospital. ipCD was defined as recurrent or refractory perianal fistula within 6 months post-surgery, without evidence of luminal disease, and responsive to biologic treatment.</p><p><b><i>Results:</i></b> Of 32 CD with perianal fistula and 33 recurrent CGD, 33 had MRI-confirmed CPF (9 ipCD, 9 pCD, 15 CGD). Compared to pCD, ipCD patients were older at diagnosis (38 vs 27 years,p=0.020), had longer disease duration (64 vs 8 months,p=0.006), higher hemoglobin (13.9 vs 11.1 g/dL,p<0.001), lower fecal calprotectin (268 vs 2498 mg/kg,p=0.014), and more complex fistula type (88.9% vs 28.6%,p=0.031). Compared to CGD, ipCD patients were younger at diagnosis (38 vs 47 years,p=0.032), had more branched fistula (88.9% vs 46.7%,p=0.011), internal opening located in a deeper position from the anal verge (3.1 vs 1.2 cm,p=0.005), and higher rate of associated abscess formation (88.9% vs 46.7%,p=0.011). ipCD patients had lower 6-month fistula remission after biologic treatment than pCD (11.1% vs 100%,p=0.007).</p><p><b>PP-02-097</b></p><p><b>Rescue Treatment of Ulcerative Colitis in Patients Unresponsive to Biologics and Recent Small Molecule treatment</b></p><p><b>Stephen Wolman</b> and Victoria Boquiren</p><p><i>Toronto General Hospital, Toronto, Canada</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Many patients with ulcerative colitis respond to 5-ASA, infliximab, ustekinumab, vedolimumab, risankizumab, mirikizumab, tofacitinib or upacitinib. Still, some patients do not respond to these medications as single agents.</p><p><b><i>Case Report:</i></b> In three patients who have pancolitis and have failed single agents, we tried combination therapy. We chose a biologic that had some impact, although minimal, and combined it with an oral JAK inhibitor. We chose the biologic with low risk of infection side effects. Patient #1 has been controlled for 3 years with no side effects. Patients 2 & 3 are only treated for three months with no significant side effect. (See results table)</p><p><b><i>Discussion:</i></b> Combinations of low risk biologics combined with JAK inhibitors should be considered in severe ulcerative colitis before colectomy.</p><p><b>PP-02-098</b></p><p><b>Mitigation effect of Lactococcus formosensis on mice with dextran sulfate sodium induced ulcerative colitis</b></p><p><b>女士 Qiuyan Wu</b>, Weilong Zhong and Bangmao Wang</p><p><i>Tianjin Medical University General Hospital, 天津, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Lactococcus Formosan is a bacterium isolated from the liver and lymph nodes of patients with severe constipation and belongs to the genus Lactococcus. The aim of this study was to investigate the role of Lactococcus formosanus in ulcerative colitis and its mechanisms.</p><p><b><i>Materials and Methods:</i></b> The colitis model was induced in C57BL/6 mice using dextran sulfate sodium (DSS).Mice were gavaged with sterile PBS or Lactococcus Formosan. Bodyweight, colon length、diarrhea severity, intestinal permeability and colonic histopathology of mice were examined. Real-time qPCR and enzyme-linked immuno sorbent assay were used to detect the content of Inflammatory factors(IL-1β, IL-6, TNF-α, DAO)in mouse serum. The levels of intestinal tight junction related proteins(occludin and ZO-1) in colon tissue were analyzed through Western Blot and immunofluorescent.</p><p><b><i>Results:</i></b> Supplementation with Lactococcus formosanus significantly attenuates weight loss and colon shortening, reduces colonic inflammation, ameliorates epithelial damage and enhances intestinal barrier integrity in mice with colitis. Compared to the DSS group, the inflammatory cytokines IL-1β, IL-6, and TNF-α in Lactococcus Formosan group were reduced. In addition, proteins linked to TJ were elevated after Lactococcus formosanus intervention.</p><p><b><i>Conclusion:</i></b> This study demonstrate that Lactococcus formosanus effectively alleviated DSS-induced colitis in mice by repairing the mucosal barrier and maintaining the intestinal microecological balance.</p><p><b>PP-02-099</b></p><p><b>Global Air Pollution and Inflammatory Bowel Disease Incidence: A GBD-based Model Analysis</b></p><p><b>Xiang Xu</b><sup>1,2</sup>, Pengguang Yan<sup>1</sup> and Jingnan Li<sup>1</sup></p><p><sup>1</sup><i>Peking Union Medical College Hospital, China;</i> <sup>2</sup><i>Peking Union Medical College, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To establish a mathematical model between air pollutants and IBD incidence, and clarify the impact of air pollution on IBD.</p><p><b><i>Materials And Methods:</i></b> Data on annual air pollutant concentrations (household air pollution (HAP), NO2, O3, PM2.5), socio-demographic index (SDI), and age-standardized rate of IBD incidence (ASIR) for 204 countries and regions from 1990 to 2020 were collected from GBD database. Spearman rank correlation analysis was conducted to explore the correlation. A random forest regression model was employed to establish the fitting model.</p><p><b><i>Results:</i></b> From 1990 to 2020, the global ASIR of IBD initially increased, reaching a peak of 4.72 cases per 100,000 people in 2010, then declined to 4.45 cases per 100,000 people. ASIR was positively correlated with SDI (r = 0.58) and NO2 (r = 0.53), and negatively correlated with HAP (r = -0.56) (p < 0.01). The random forest regression model exhibited a strong fit to the ASIR, with RMSE, MAE, and MAPE of 0.45, 0.20, and 9.74%, respectively. Feature importance analysis revealed that SDI was the most important factor in predicting ASIR, followed by HAP, PM2.5, O3, and NO2, suggesting that air pollution might influence ASIR, with indoor air quality potentially outweighing outdoor air quality.</p><p><b><i>Conclusions:</i></b> This study fills the gap between global air pollution and IBD epidemiology. Based on the random forest regression model, HAP, NO2, O3, PM2.5, and SDI were used to fit the trend of IBD incidence, offering predictive value and a scientific basis for the future study of IBD.</p><p><b>PP-02-100</b></p><p><b>ABA from B.licheniformis ameliorates DSS-induced colitis in mice by regulating gut microbiota and immune response</b></p><p><b>Zeyan Xu</b><sup>1</sup>, Lijiang Zhao<sup>1</sup>, Ruihua Shi<sup>1,2</sup> and Daqing Gao<sup>1</sup></p><p><sup>1</sup><i>Southeast University, Nanjing, China;</i> <sup>2</sup><i>Zhongda Hospital, Southeast University, Nanjing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>ABA is a secondary metabolic and sesquiterpene product which has been shown modulation in T-cell classification and adhesion molecule expression to ameliorate gut inflammation in experimental mice with DSS-induced colitis. Some evidences suggest that gut microbiota is an important factor in regulating immune responses in IBD. ABA treatment maybe modulate gut microbiota to affect IBD progression. However, the ABA content in a living thing is so very low that it is difficult for ABA to be purified. The bcaba1 gene encodes a cytochrome P450 monooxygenase and is associated with ABA biosynthesis in fungus Botrytis cinerea (B. cinerea). In this study, we explored whether the Bl-cyp strain which capable of enhancing its ABA production, could be more effective in ameliorating DSS-induced colitis by regulating the gut microbiota and host immunity. Our present study provide evidences for the prevention and treatment against IBD.</p><p><b>PP-02-101</b></p><p><b>Prescription cases of Biologics and Small Molecule Therapies for our ulcerative colitis patients</b></p><p><b>Yoshiharu Yamaguchi</b></p><p><i>Aichi Medical University School Of Medicine, Nagakute, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ulcerative colitis (UC) predominantly impacts the youth, marked by alternating flare-ups and remissions. Despite treatments like biologics (BIO) and JAK inhibitors (JAK-I), a definitive cure for moderate to severe IBD remains elusive. This study examines BIO and JAK-I efficacy and continuation in real-world UC patients, considering the diverse patient backgrounds.</p><p><b><i>Materials and Methods:</i></b> We analyzed 81 UC patients treated with BIO (50) and JAK-I (31) from January 2019 to December 2023. Those discontinuing treatment within three months were ‘No responders,’ and those continuing beyond three months but less than a year were ‘Partial responders.’</p><p><b><i>Results:</i></b> Non-response rates were 25.9% for BIO and 10.8% for JAK-I, without a significant difference (p=0.108). The BIO group had a significantly higher partial response (40.7% vs. 10.0%, p<0.05). Kaplan-Meier analysis indicated a higher continuation rate for JAK-I. No notable difference in serious adverse events, including cardiovascular issues or herpes zoster incidence, was found between groups.</p><p><b><i>Conclusion:</i></b> UC patients on BIO faced more challenges in continuing treatment than those on JAK-I. While serious adverse events were similar for both, proactive measures to reduce risks like thrombosis are recommended.</p><p><b>PP-02-102</b></p><p><b>Salivary Exosomes in Colitis: Unraveling a Novel Mechanism of Oral-Intestinal Communication</b></p><p><b>Congyi Yang</b>, Jingyi Chen and Ning Chen</p><p><i>Peking University People's Hospital, Beijing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Inflammatory bowel disease (IBD) involves not just intestinal inflammation but also extraintestinal manifestations, including frequent involvement of the oral cavity.This study aims to unearth the mechanisms underlying long-distance communication between the mouth and gut and the precise role of salivary exosomes.</p><p><b><i>Materials and Methods:</i></b> Salivary exosomes were isolated from healthy controls(n=25)and patients with IBD(n=105) using ultracentrifugation. In vivo, we established a colitis mouse model. In vitro, THP-1 cells and Caco-2 cells were exposed to salivary exosomes separately.</p><p><b><i>Results:</i></b> Salivary exosomes with DIL tags primarily built up in the mouse colon six hours after gavage. In the DSS mouse model, we observed that salivary exosomes from patients with active IBD (active IBD-Sexos) exacerbated colitis, while those from IBD patients in remission (remission IBD-Sexos) did not. The funding indicates that salivary exosomes may play a possible role in the regulation of macrophage polarization towards the M1 phenotype, disruption of intestinal epithelial function, and alteration of the intestinal flora. Co-culturing THP-1 cells or Caco-2 cells with active IBD-Sexos induces an inflammatory reaction, resulting in an elevated release of proinflammatory cytokines including TNF-a, IL-6, and IL-1β. Additionally, we identified differentially expressed microRNAs in active IBD-Sexos compared to exosomes extracted from IBD patients in remission and healthy control, indicating a unique immune and inflammatory signature associated with active IBD.</p><p><b><i>Conclusion:</i></b> Our findings demonstrate the significance of salivary exosomes in bridging the oral and intestinal compartments, independent of microbes or immune cells. Furthermore, it shows that active IBD-Sexos actively promote the progression of colitis.</p><p><b>PP-02-103</b></p><p><b>Colonic CD4+ T cell senescence contributes to the progression of colitis in aged mice</b></p><p><b>Yang Zhang</b>, Jun Xu, Shan Cao, Yiken Lin and Yulan Liu</p><p><i>Peking University People's Hospital, Beijing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Aging renders the elderly increasingly susceptible to inflammatory and autoimmune disease. Herein, we sought to determine the role of immunosenescence in age-dependent colitis and to explore the underlying mechanisms.</p><p><b><i>Methods:</i></b> Young (2-3 months), middle-aged (10-12 months), and aged (20-24 months) mice were established models of colitis by 2% dextran sulfate sodium salt (DSS) treatment. We detected the senescence-associated β-galactosidase (SA-βGal) activity in lymphocytes isolated from colon of mice. Single-cell and bulk RNA-seq of colonic immune cells in mice with different ages were performed to investigate the precise molecular mechanisms. CD4+CD25-CD45RBhi T cell adoptive transfer model was used to further analyze the role of senescent CD4+ T cells in colitis.</p><p><b><i>Results:</i></b> Ageing increased the severity of DSS-induced colitis. The greatest age-associated increases of SA-βGal activity were observed in colonic CD4+ T cells. Aged colonic CD4+ T cells generate higher levels of IFN-γ and IL-17 comparing to young colonic CD4+ T cells, especially in status of colitis. Single-cell analysis revealed that aging increased colonic CD4+ TEM cells, which were associated with T cell-mediated cytotoxicity and cytokine-mediated signaling pathway. Splenic CD4+ TEM cells isolated from aged colitis mice were confirmed to involve in cellular senescence, Th17 differentiation and inflammatory signaling pathway through bulk RNA-seq. Adoptive transfer of aged CD4+ T cells to Rag-/- mice accelerated CD4+ T cell production of inflammatory cytokines and induced more severe colitis compared with young CD4+ T cells transfer.</p><p><b><i>Conclusion:</i></b> These results provide a significant insight into the contribution of senescent CD4+ T cells to age-dependent colitis.</p><p><b>PP-02-104</b></p><p><b>USP9X restrains mucosal inflammation by orchestrating the intestinal monocyte to macrophage maturation via deubiquitinating STAT1</b></p><p><b>先生 Tao Zhang</b> and Hailong Cao</p><p><i>Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Inflammatory bowel disease (IBD) is chronic inflammatory disorder characterized by the crucial involvement of macrophages. Ubiquitin-specific protease 9X (USP9X) is a deubiquitinating enzyme that regulates protein stability and activity by removing ubiquitin tags from proteins. This study aims to comprehensively understand the molecular mechanisms by which USP9X regulates macrophage function during colonic inflammation.</p><p><b><i>Materials and Methods:</i></b> The expression level of USP9X in macrophages of IBD patients was analyzed using sequencing data from single-cell sequencing data. Mice with a macrophage-specific knockout of USP9X were utilized to study the effect of macrophage USP9X on the development of colitis.</p><p><b><i>Results:</i></b> USP9X expression was reduced in colonic macrophages of IBD patients. Upon LPS stimulation, USP9X expression significantly decreased in PBMCs, BMDMs, RAW264.7, and THP-1 cells. Knockdown of USP9X led to increased expression of M1 macrophage markers and pro-inflammatory cytokines, enhanced phagocytosis, and migration. In the colitis mouse model, macrophage-specific USP9X deficiency exacerbated colitis and elevated M1 macrophages in the colonic lamina propria.Ubiquitin proteomics revealed that USP9X silence increased ubiquitination levels at K379 and K544 on STAT1. Co-immunoprecipitation and immunofluorescence confirmed the interaction between USP9X and STAT1. USP9X deletion enhanced STAT1 phosphorylation and accelerated its nuclear translocation, which is critical for M1 macrophage polarization.</p><p><b><i>Conclusion:</i></b> Macrophage-specific USP9X deficiency induces M1-like macrophage polarization, impairs macrophage maturation, and exacerbates intestinal inflammation.</p><p><b><i>Keywords:</i></b> Inflammatory bowel disease; post-translational modification; macrophage polarization.</p><p><b>PP-02-105</b></p><p><b>Global, regional, and national burden of inflammatory bowel disease from 1990 to 2021</b></p><p><b>Claire Chenwen Zhong</b><sup>1</sup>, Ziwei Huang<sup>2</sup>, Xingxin Gu<sup>3</sup>, Suwen Shi<sup>4</sup>, Xiangyi Meng<sup>5</sup>, Junjie Hang<sup>6</sup>, Jianli Lin<sup>7</sup>, Yu Li<sup>8</sup> and Junjie Huang<sup>1</sup></p><p><sup>1</sup><i>The Chinese University Of Hong Kong, Hong Kong, China;</i> <sup>2</sup><i>Department of Physics, Boston University, Boston, Massachusetts, 02215, USA;</i> <sup>3</sup><i>College of Professional Studies, Northeastern University, Boston, Massachusetts, 02115, USA;</i> <sup>4</sup><i>Department of Chemistry, Boston University, Boston, Massachusetts, 02215, USA;</i> <sup>5</sup><i>Department of Physics and Astronomy, Northwestern University, Evanston, Illinois, 60208, USA;</i> <sup>6</sup><i>Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, Guangdong, 518000, China;</i> <sup>7</sup><i>Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100080, China;</i> <sup>8</sup><i>Department of Computer Science and Engineering, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, 999077, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Inflammatory bowel disease (IBD) presents a significant global health challenge, exhibiting varied levels and trends across countries and regions. Understanding these geographical disparities is essential for developing effective prevention and treatment strategies.</p><p><b><i>Methods:</i></b> This study utilized data from the Global Burden of Diseases Study 2021 to investigate IBD trends across 204 countries and territories from 1990 to 2021. Annual case data and age-standardized rates(ASRs) were analyzed to assess IBD incidence, mortality, and disability-adjusted life-years(DALYs). Total percentage changes were used to track trends in incidence, mortality, and DALY rates over time.</p><p><b><i>Results:</i></b> In 2021, the global incidence of IBD was estimated at approximately 0.38 million cases(95% uncertainty interval[UI]: 0.33-0.44 million), resulting in 1.51 million DALYs(95% UI: 1.31-1.75 million). From 1990 to 2021, the global age-standardized incidence rate increased by 5%, while age-standardized mortality and DALY rates decreased by 13% and 16%, respectively. Regionally, Australasia had the highest age-standardized incidence rate in 2021(19.7 per 100,000 population), whereas Central Latin America reported the lowest(0.57 per 100,000). At the national level, Canada showed the highest age-standardized incidence rate(26.8 per 100,000 population), followed by Greenland(24.6 per 100,000). Netherlands recorded the highest age-standardized death rate(2.21 per 100,000 population), with Singapore having the lowest (0.026 per 100,000).</p><p><b><i>Conclusion:</i></b> The findings underscore the escalating global burden of IBD and highlight the need for targeted public health interventions. Effective strategies should focus on strengthening healthcare systems, enhancing specialized training, and promoting early detection and management of IBD to alleviate its impact on health outcomes and socioeconomic well-being globally.</p><p><b>PP-02-106</b></p><p><b>Study on the relationship between sleep disorders and autonomic function in patients with ulcerative colitis</b></p><p><b>Jiaming Zhou</b>, Jian Wan and Kaichun Wu</p><p><i>Xijing Hospital Of Digestive Diseases, Air Forth Medical University, Xi’an, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the impact of sleep disorders on autonomic function, psychological symptoms and quality of life in ulcerative colitis (UC) patients.</p><p><b><i>Methods:</i></b> UC patients from Xijing Hospital were prospectively enrolled from January 2024 to June 2024. The Pittsburgh Sleep Quality Index(PSQI) was used to assess sleep quality. The Generalized Anxiety Disorder-7(GAD-7), the Patient Health Questionnaire-9(PHQ-9), and the Inflammatory Bowel Disease Questionnaire(IBDQ) were used to evaluate psychological symptoms and quality of life. Heart rate variability was measured to assess autonomic function.</p><p><b><i>Results:</i></b> 75 patients with active UC were included, with a mean age of 41.44 years, a higher proportion of males than females(60.0% vs 40.0%), and an average disease duration of 6.34 years. 50.7% of UC patients were in the mild active stage and 49.3% in the moderate to severe active stage. The prevalence of sleep disorders was 64.0%. Compared to UC patients without sleep disorders, those with sleep disorders had a higher proportion of comorbid anxiety(P=0.011) and depression(P<0.001), higher GAD-7 scores(P=0.009) and PHQ-9 scores(P<0.001), and lower IBDQ scores(P=0.008). HF, reflecting vagal nerve activity, was significantly lower in the UC group with sleep disorders(0.32±0.16 vs 0.41±0.16, P=0.027), while the LF/HF ratio was significantly higher(3.00±2.11 vs 2.02±1.76, P=0.024).</p><p><b><i>Conclusions:</i></b> UC patients with sleep disorders have more severe anxiety and depression, poorer quality of life, and increased autonomic dysfunction, as evidenced by lower vagus nerve activity and higher sympathetic nerve activity. Exploring whether sleep disorders promote the development and progression of UC by affecting autonomic function is a direction for future research.</p><p><b>PP-02-107</b></p><p><b>Rifaximin Therapy Versus Low FODMAP Diet in Irritable Bowel Syndrome: A Randomised Controlled Trial</b></p><p><b>Wah Loong Chan</b><sup>1</sup>, Kee Huat Chuah<sup>1</sup>, Qing Yuan Loo<sup>1</sup>, Audrey Joe Chii Loh<sup>1</sup>, Wen Xuan Hian<sup>1</sup>, Xin Hui Khoo<sup>1</sup>, Sarala Panirsheeluam<sup>3</sup>, Hazreen Abdul Majid<sup>2</sup> and Sanjiv Mahadeva<sup>1</sup></p><p><sup>1</sup><i>Gastroenterology and Hepatology Unit, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Department of Social Preventive Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;</i> <sup>3</sup><i>Staff & Student Health Unit, University Malaya Medical Centre, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Dysbiosis contributes to the pathophysiology of irritable bowel syndrome (IBS), with antibiotic and dietary interventions potentially altering gut microbiota and alleviating symptoms. This study aims to compare the efficacy of Rifaximin versus a low FODMAP (fermentable oligosaccharides, disaccharides, monosaccharides, and polyols) diet (LFD) in IBS patients.</p><p><b><i>Materials and Methods:</i></b> In this single-blinded, randomized controlled trial, IBS patients were assigned to either Rifaximin 400 mg TDS for 2 weeks or a dietitian-guided LFD for 4 weeks. The primary outcome was the proportion of patients with composite symptom improvement (abdominal pain/discomfort and bowel symptoms) at week 2 or 4. Secondary outcomes included improvements in IBS-symptom severity scale (IBS-SSS), global IBS symptoms, bloating, abdominal pain, stool consistency, and quality of life.</p><p><b><i>Results:</i></b> A total of 84 patients (Rifaximin, n=43; LFD, n=41) were recruited (mean age: 49 years, 52.4% female, IBS-diarrhea 71.4%). The proportion of patients achieving composite symptom improvement did not differ significantly between the groups (67.4% vs 53.7%, p=0.196). The Rifaximin group showed significant improvement in bloating and stool frequency compared to the LFD group (93.0% vs 73.2%, p=0.015; 88.4% vs 68.3%, p=0.025). Both groups showed significant improvements in IBS-SSS and EQ-5D scores (p<0.001), with no statistically significant differences in the degree of change between the groups. There were no significant differences in improvement of abdominal pain and global symptoms. Sub-group analysis of IBS-D patients yielded similar results.</p><p><b><i>Conclusion:</i></b> Both Rifaximin and LFD were effective in treating IBS, but Rifaximin was superior in improving bloating and stool frequency.</p><p><b>PP-02-109</b></p><p><b>Epidemiology and risk factors of gastrointestinal-symptoms and post-infectious bowel disorders as sequelae in COVID-19 patients</b></p><p><b>Gyung Mo Goo</b><sup>1</sup>, Kwang Woo Kim<sup>1</sup>, Ji Young Baek<sup>1</sup>, Bo Kyung Kim<sup>1</sup>, Hee Joon Jang<sup>1</sup>, Dong Kee Jang<sup>1</sup>, Su Hwan Kim<sup>1</sup>, Yong Jin Jung<sup>1</sup>, Ji Won Kim<sup>1</sup>, Byeong Gwan Kim<sup>2</sup>, Kook Lae Lee<sup>1</sup> and Hyoun Woo Kang<sup>1</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul National University College of Medicine, Seoul, Republic of Korea;</i> <sup>2</sup><i>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Gastrointestinal manifestation of COVID-19 infection is now well known. However, there is few study about incidence of bowel disorders including post-infectious irritable bowel syndrome (PI-IBS), functional constipation, and functional diarrhea after long-term follow up. We investigate to evaluate the risk factors related with long-term post-COVID-19 (Coronavirus disease-2019) bowel disorders.</p><p><b><i>Methods:</i></b> This single-center, prospective, observational study enrolled individuals infected with SARS-CoV-2 between November 2021 and February 2023. Baseline characteristics were collected at the time of infection, and follow-up surveys assessed bowel disorders based on ROME IV criteria.</p><p><b><i>Results:</i></b> Among 149 initially asymptomatic subjects, 22 developed bowel disorders (7 PI-IBS, 5 functional constipation, 10 functional diarrhea) over a mean follow-up period of 15.5 months. The mean age at infection was 63.2 years, with males comprising 47.0% of the cohort. Alcohol consumption was significantly higher in the group with post-COVID-19 bowel disorders compared to controls (27% vs. 9%, p = 0.018). After adjusting for COVID-19 severity, multivariable logistic regression confirmed a significant association between alcohol consumption and post-COVID-19 bowel disorders. Subgroup analysis showed no significant differences in PI-IBS and functional constipation groups compared to controls, but the functional diarrhea group exhibited notable disparities in white blood cell count, absolute neutrophil count, and alcohol consumption rate (p = 0.044, p = 0.028, p = 0.020, respectively).</p><p><b><i>Conclusion:</i></b> This study highlights a significant relationship between alcohol consumption and bowel disorders, particularly diarrhea, as long-term consequences SARS-CoV-2 infection. Further research is warranted to explore additional risk factors and elucidate the relationship between COVID-19 and gastrointestinal disorders.</p><p><b>PP-02-110</b></p><p><b>Symptomatology aspects differentiating irritable bowel syndrome (IBS) and organic diseases: age and sex effect</b></p><p><b>Amal Arifi Hidayat</b><sup>1,4</sup>, Hoda M Malaty<sup>3,2</sup>, Langgeng Agung Waskito<sup>1,4</sup>, Titong Sugihartono<sup>2</sup>, Pangestu Adi<sup>2</sup>, Husin Thamrin<sup>2</sup>, Amie Vidyani<sup>2</sup> and Muhammad Miftahussurur<sup>2,4</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>3</sup><i>Department of Medicine, Baylor College of Medicine, Houston, United States;</i> <sup>4</sup><i>Helicobacter pylori and Microbiota Study Group, Institute Tropical Disease, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To investigate the clinical features that distinguish between irritable bowel syndrome (IBS) and organic diseases among patients with typical IBS symptoms.</p><p><b><i>Materials and Methods:</i></b> Patients fulfilling the Rome IV IBS criteria were assessed for their demographic data, detailed bowel symptoms, and alarm features. All patients underwent routine laboratory tests and a colonoscopy investigation. Based on the colonoscopy findings, patients were assigned into two groups: IBS or organic disease group. Univariate and multivariate analysis were performed to compare clinical features between the two groups.</p><p><b><i>Results:</i></b> Of the 83 patients recruited, 43 (51.8%) were classified into IBS group, and 40 (48.2%) were into organic disorders: 30 (75%) IBD, 2 (5%) malignancy, 6 (15%) polyps, and 2 (5%) diverticulitis. Patients aged ≥40 years demonstrated a higher prevalence of organic diseases, irrespective of sex, with a statistically significant difference (36.6% vs. 59.5%, p<0.05). Alarm features were highly prevalent in both groups and failed to demonstrate significant differences (53.5% vs. 47.5%, p>0.05). Among patients with IBS symptoms, a higher frequency of abdominal pain (OR=2.24, 95%CI 1.02-4.89, p<0.05) and pain that does not improve after defecation (OR=21.01, 95%CI 4.83-91.33, p<0.01) were independently correlated with the presence of organic diseases.</p><p><b><i>Conclusion:</i></b> Half of the patients presenting with typical IBS symptoms had organic diseases. Colonoscopies should be considered for all patients aged ≥40 years with IBS symptoms, regardless of sex or the presence of alarm features. More frequent abdominal pain and pain that does not improve after defecation are predictors of the presence of organic diseases.</p><p><b>PP-02-111</b></p><p><b>Prevalence of NAFLD and MAFLD in patients with Irritable Bowel Syndrome</b></p><p><b>Musab Khalil</b></p><p><i>Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Both NAFLD and IBS may share common risk factors. Data are sparse regarding the frequency of Metabolic Dysfunction associated Fatty Liver Disease (MAFLD) among Irritable Bowel Syndrome (IBS) patients.</p><p><b><i>Methods:</i></b> We have conducted a cross-sectional comparative study at the outpatient department of SRNGIH to find out the frequency of Non-Alcoholic Fatty Liver Disease (NAFLD) and Metabolic dysfunction Fatty Liver Disease (MAFLD) among patients with Irritable Bowel Syndrome (IBS).</p><p><b><i>Results:</i></b> Out of 219 patients,121 patients had IBS. Irritable Bowel Syndrome - Diarrhea Predominant (IBS-D) was the most prominent type (55%). One-third (n=39) of the IBS (32.23%) patients had NAFLD. One-tenth [n=12] had MAFLD. Significantly younger patients had NAFLD among IBS patients compared to non-IBS patients [p=0.023]. Non-IBS patients had significantly more Low-Density Lipoprotein (LDL) compared to IBS patients (p=0.040). Serum triglycerides were higher among IBS patients having NAFLD compared to non-IBS patients having NAFLD (p=0.022). Serum Alanine Aminotransferase (S. ALT) was significantly higher in IBS patients with NAFLD than in IBS patients without NAFLD (p=0.006). Body Mass Index (BMI) was significantly higher in IBS patients with MAFLD than in IBS patients without MAFLD. Total and LDL cholesterol were significantly higher in IBS with MAFLD patients than in IBS without MAFLD.</p><p><b><i>Conclusion:</i></b> One-third of Irritable Bowel Syndrome (IBS) patients had Nonalcoholic Fatty Liver Disease (NAFLD). Metabolic dysfunction associated with Fatty Liver Disease (MAFLD) was less prevalent among patients with IBS.</p><p><b>PP-02-112</b></p><p><b>Study on the Therapeutic Effects and Mechanisms of Gintonin in Irritable Bowel Syndrome</b></p><p>Na Ri Choi<sup>1,2</sup>, Seok Jae Ko<sup>3,4</sup>, Joo Hyun Nam<sup>5,6</sup>, Woo-Gyun Choi<sup>1</sup>, Jong Hwan Lee<sup>7</sup>, Seung-Yeol Nah<sup>8</sup>, Jae Woo Park<sup>3,4</sup> and <b>Byungjoo Kim</b><sup>1</sup></p><p><sup>1</sup><i>Department of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, South Korea;</i> <sup>2</sup><i>Department of Korean Medical Science, Pusan National University School of Korean Medicine, Yangsan, South Korea;</i> <sup>3</sup><i>Department of Clinical Korean Medicine, Graduate School of Kyung Hee University, Seoul, South Korea;</i> <sup>4</sup><i>Department of Gastroenterology, College of Korean Medicine, Kyung Hee University, Seoul, South Korea;</i> <sup>5</sup><i>Department of Physiology, Dongguk University College of Medicine, Kyungju, South Korea;</i> <sup>6</sup><i>Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang, South Korea;</i> <sup>7</sup><i>Department of Biomedical Engineering, Dong-Eui University College of Engineering, Busan, South Korea;</i> <sup>8</sup><i>Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Irritable bowel syndrome (IBS) is a gastrointestinal disease accompanied by changes in bowel habits without any specific cause. Gintonin is a newly isolated glycoprotein from ginseng that is a lysophosphatidic acid (LPA) receptor ligand.</p><p><b><i>Materials and Methods:</i></b> To investigate the efficacy and mechanisms of action of gintonin in IBS, we developed a zymosan-induced IBS murine model. In addition, electrophysiological experiments were conducted to confirm the relevance of various ion channels.</p><p><b><i>Results:</i></b> In mice, gintonin restored colon length and weight to normal and decreased stool scores, whilst food intake remained constant. Colon mucosal thickness and inflammation-related tumor necrosis factor-α levels were decreased by gintonin, along with a reduction in pain-related behaviors. In addition, the fecal microbiota from gintonin-treated mice had relatively more Lactobacillaceae and Lachnospiraceae and less Bacteroidaceae than microbiota from the control mice. Moreover, gintonin inhibited transient receptor potential vanilloid (TRPV) 1, TRPV4, and voltage-gated Na+ 1.5 channels associated with visceral hypersensitivity.</p><p><b><i>Conclusion:</i></b> These results suggest that gintonin may be one of the effective components in the treatment of IBS.</p><p><b>PP-02-113</b></p><p><b>Food Avoidance, COVID-19 Infection and Post-Infectious Functional Gastrointestinal Disorders: Is There A Link?</b></p><p><b>Shanthi Krishnasamy</b><sup>1</sup>, Petrik@Ramesh Periyasamy<sup>2</sup>, Deborah Chia Hsin Chew<sup>2</sup> and Assoc Prof Kewin Siah<sup>3</sup></p><p><sup>1</sup><i>Faculty of Health Sciences, National University of Malaysia, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Department of Medicine, Hospital Canselor Tuanku Mukhriz, National University Hospital Malaysia, Cheras, Malaysia;</i> <sup>3</sup><i>Department of Gastroenterology, National University of Singapore, Lower Kent Ridge Rd, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> COVID-19 increases the risk of Post-infectious Functional Gastrointestinal Disorders (PI-FGID). This case-control study identified the frequency of FGID, and determine its impact on the quality of life, psychosocial status and eating habits of Malaysian patients.</p><p><b><i>Methods:</i></b> A total of 227 subjects, comprising of 116 healthy controls and 111 COVID-19 cases completed the study using validated and translated questionnaires. Chi-square test was performed to determine relationship between categorial variables.</p><p><b><i>Results:</i></b> A total of 3.1% cases and 2.2% controls developed PI-FGID over 1 month post-COVID-19 infection with an odds ratio of 1.49 with no significant differences between groups (p>0.05). There was a significant relationship between FGID and quality of life with regards to level of pain/discomfort (p=0.037) and level of anxiety/depression (p=0.000) among cases. Psychological status was significantly correlated with FGID (p=0.027) particularly those who have experienced abuse. A total of 14.1% (n=227) subjects reported improvement in gastrointestinal symptoms following food avoidance whereby fructan was the most common FODMAP that was avoided.</p><p><b><i>Conclusions:</i></b> COVID-19 increases the risk of PI-FGID impacting quality of life. Patients with stressful life events have an increased risk and may avoid trigger foods high in FODMAPs.</p><p><b>PP-02-114</b></p><p><b>A case report: Crohn’s Disease Diagnosed After Rectal Mucosa-associated Lymphoid Tissue (MALT) Lymphoma</b></p><p><b>Hua Yen Ling</b>, H Lin and Jacqueline Zhiling Yang</p><p><i>Tan Tock Seng Hospital, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> We report a case of Crohn’s disease (CD) diagnosed after MALT treatment and Cytomegalovirus (CMV) disease.</p><p><b><i>Case Description:</i></b> A previously well 60-year-old Malay male presented with 2 weeks of haematochezia. Colonoscopy showed circumferential mucosal irregularity of lower rectum. Biopsy confirmed rectal MALT. He underwent 15 cycles of definitive radiotherapy and computed topography 6 months post treatment showed stable changes. However, he still had intermittent mild haematochezia despite being in disease remission. Flexible sigmoidoscopy that was done reported radiation proctitis without biopsy taken. He was prescribed one week of hydrocortisone enema.</p><p>A trip to rural village in Indonesia worsened his haematochezia necessitating hospitalisation. Stool infective screen was negative. Repeat sigmoidoscopy showed multiple patchy deep, cratered ulcers with inflamed mucosa throughout. Histology showed dense active chronic inflammation and ulcerations with inclusion bodies. He completed 3 weeks of Cytomegalovirus treatment.</p><p>Repeat colonoscopy was done to document CMV resolution although haematochezia resolved. It showed persistent scattered ulcers, pseudopolyps with loss of vascular pattern from rectum to distal ascending colon. Histology did not suggest any residual lymphoma; skip segment and patchy inflammation were more suggestive of Crohn’s disease. He was started on mesalazine enema with improvement of symptoms.</p><p><b><i>Discussion:</i></b> The association between CD and MALT lymphoma remains controversial. Inflammatory bowel disease (IBD) associated lymphoma was proposed to be related to treatment (thiopurine/anti-TNF), Epstein-Barr virus and chronic inflammation. However in our case, lymphoma was diagnosed before CD and it is difficult to determine causal effect as no whole colon biopsies were taken during index colonoscopy.</p><p><b>PP-02-115</b></p><p><b>Abnormalities in anorectal physiology amongst constipated individuals – data from a Singaporean centre</b></p><p><b>Valerie Yeap</b> and Andrew Ong Ming Liang</p><p><i>Singhealth / Singapore General Hospital, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> Chronic constipation is a common problem in gastroenterology, yet it remains a challenge to treat. Anorectal physiology testing aids in the evaluation and management of constipation, and the it provides enables treatment plans to be tailored accordingly.</p><p>Our aim was to evaluate the abnormalities in anorectal function amongst patients with chronic constipation, to achieve better understanding of the pathophysiological mechanisms underlying their symptoms. These anorectal manometry (ARM) findings were then compared against the various forms of treatment patients received.</p><p><b><i>Materials and methods:</i></b> This was a retrospective analysis of all patients who underwent ARM testing for constipation symptoms between January 2021 and December 2023 in Singapore General Hospital. The ARM parameters assessed were anal sphincter pressures ( rest and squeeze), recto-anal coordination during simulated defecation, rectal sensation and recto-anal reflex activity. Electronic clinic notes and prescription charts were reviewed to evaluate how patients’ symptoms were simultaneously managed.</p><p><b><i>Results:</i></b> A total of 223 patients underwent ARM for evaluation of constipation, of which 62% (N=138) were female. The median age was 59. 80% were of Chinese ethnicity, the remaining were Malay (6%), Indian (6%) and of other ethnicities (8%). Dyssynergia was the most common finding (70%,N=157), followed by rectal hypersensitivity (57%,N=128). A combination of rectal hypersensitivity and dyssynergia was seen in 39%(N=87) of patients.</p><p>The most common treatment undertaken was laxatives (92%,N=204), followed by biofeedback therapy (60%,N=134). Prucalopride was prescribed in (21%,N=47) and neuromodulators only in (10%,N=23).</p><p><b><i>Conclusion:</i></b> Rectal hypersensitivity and dyssynergia are common and often co-exist amongst patients with chronic constipation. The treatment of their symptoms should therefore be tailored accordingly. More can be done to address rectal hypersensitivity amongst constipated individuals.</p><p><b>PP-02-116</b></p><p><b>Immune cells and irritable bowel syndrome: A bidirectional Mendelian randomization study of their causal relationship</b></p><p><b>Wan Zhou</b><sup>1</sup> and Pan Xu<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, University-Town Hospital of Chongqing Medical University, Chongqing, China;</i> <sup>2</sup><i>Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The causal relationship between immunophenotype and irritable bowel syndrome (IBS) remains unclear. We conducted a bidirectional Mendelian randomization (MR) study using genome-wide association data (GWAS) to evaluate this association.</p><p><b><i>Method:</i></b> Leveraging GWAS data, we explored 731 immunophenotypes' causal interplay with IBS. The inverse variance weighting (IVW) method was employed as the primary analytical approach. The MR-PRESSO outlier test, Cochran's Q heterogeneity test, and MR Egger intercept test were utilized for sensitivity analyses.</p><p><b><i>Results:</i></b> After false discovery rate (FDR) correction, four immunophenotypes were found to be linked with an increased risk of IBS: CD27 on CD24+ CD27+ B-cell (odds ratio [OR]=1.04; 95% confidence interval [CI], 1.02–1.06; p < 0.001); CD27 on IgD- CD38- B-cell (OR=1.04; 95% CI, 1.02–1.07; p < 0.001); CD27 on IgD- CD38dim B-cell (OR=1.03; 95% CI, 1.01–1.06; p < 0.001); and CD27 on sw mem B-cell (OR=1.03; 95% CI, 1.01–1.05; p < 0.001). One immunophenotype negatively correlated with the risk of IBS: IgD- CD27- %B-cell (OR=0.93; 95% CI, 0.90–0.97; p < 0.001). Statistically significant associations were not found among the 731 immunophenotypes and IBS in the reverse MR analysis.</p><p><b><i>Conclusions:</i></b> Four immunophenotypes (CD27 on CD24+ CD27+, CD27 on IgD- CD38-, CD27 on IgD- CD38dim, CD27 on sw mem B-cell) are risk factors for IBS, while one immunophenotype (IgD- CD27- %B-cell) is a protective factor against IBS, and they all belong to B cells. This finding further elucidates the role of immune factors in the risk of IBS and provides a basis for subsequent clinical investigations.</p><p><b>PP-02-117</b></p><p><b>Polyposis Syndrome - Familial Adenomatous Polyposis with rectal adenocarcinoma presenting as hematochezia: A case-report</b></p><p><b>John Derek Clutario</b>, Marc Julius Navarro and Jose Luis Matthias Sollano</p><p><i>Makati Medical Center, Pasig, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Familial Adenomatous Polyposis (FAP) is a hereditary condition characterized by the development of numerous colorectal adenomatous polyps, often in the hundreds to thousands. This autosomal dominant disorder is caused by mutations in the APC (Adenomatous Polyposis Coli) gene. Without prophylactic treatment, nearly all individuals with FAP will develop colorectal cancer by the age of 40.</p><p><b><i>Case description:</i></b> In this report, we present a case of a forty two year old female who has been having hematochezia for a year. She has only been treated as a case of grade 4 internal hemorrhoids however was not worked up further due to financial constraints. CT scan done, it revealed a semi-circumferential 6.4cm mass in the mid to low rectum hence a colonoscopy was done. Findings were multiple (estimated to be in the thousands) polypoid lesions spanning the entire colonic tract. Biopsies in the colon revealed tubular and tubulovillous adenoma and unfortunately biopsy from the rectum revealed rectal adenocarcinoma. On retrospective history taking, the patient did not have any known relatives with the same symptoms but she has already lost touch with majority of her relatives. Specimens will be sent abroad for complete genetic testing and the patient is now being prepared for total proctocolectomy.</p><p><b><i>Discussion:</i></b> This case underscores the importance of vigilant surveillance and timely intervention in individuals with FAP, as well as the clinical implications of rectal bleeding in this high-risk population. Rectal adenocarcinoma, a common complication of FAP, can present with various symptoms, among which hematochezia—rectal bleeding.</p><p><b>PP-02-118</b></p><p><b>Diverticular Bleeding: A Case Study in Diagnostic and Therapeutic Approach</b></p><p>Albert William Hotomo<sup>1</sup>, <b>Ahmad Nur Aulia</b><sup>2</sup>, Andhiky Raymonanda Madangsai<sup>2</sup> and Muhammad Firhat Idrus<sup>2</sup></p><p><sup>1</sup><i>Departement of Internal Medicine, FKKH UNDANA, Kupang, Indonesia;</i> <sup>2</sup><i>Pusat Endoskopi Saluran Cerna, RSUPN Dr. Cipto Mangunkusumo/FKUI, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Diverticular bleeding, characterized by painless hematochezia, is a prevalent gastrointestinal complication with a significant age-related incidence. Nonsteroidal anti-inflammatory drugs, aspirin, and antiplatelet agents are established risk factors. Hypertension has been associated with vascular injury, which may exacerbate the condition.</p><p><b><i>Case Description:</i></b> An 84-year-old woman presented with recurrent hematochezia 4 hours prior at around 50 mL volume. Her medical history included hypertension and routine NSAID use. She denied wasting symptoms or a history of malignancy. Physical examination revealed pale conjunctiva, tenderness of the left lower abdominal region, and digital rectal examination showed a reddish-brown stool and blood. Blood test evaluation demonstrated anemia, acute renal failure due to blood loss, and normal coagulation test. The 1st Colonoscopy showed minimal multiple diverticular bleeding at the descending colon. Hemoclip or argon plasma coagulation has been planned if there is any active bleeding from the diverticle. Omeprazole, tranexamic acid, and sucralfate were administered to alleviate minimal active bleeding from the diverticula, along with a high-fiber diet. A subsequent colonoscopy showed no active bleeding.</p><p><b><i>Discussion:</i></b> Diverticular bleeding is typically managed with a combination of medical and endoscopic treatments, with surgery reserved for severe cases. While other available pharmacologic treatments like vasopressin can reduce blood flow to the intestines, the effectiveness of tranexamic acid for lower gastrointestinal bleeding is still under investigation. Preventing diverticulosis through a high-fiber diet, adequate hydration, and regular exercise can lower the risk of bleeding.</p><p><b>PP-02-119</b></p><p><b>A Case of Disseminated Tuberculosis presenting as Massive Gastrointestinal Bleeding in an Immunocompetent Patient</b></p><p><b>Natasha Bernadine Mapa</b> and Carissa Marin</p><p><i>Makati Medical Center, Makati City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal tuberculosis (GITB) is an uncommon form of extrapulmonary tuberculosis that poses a diagnostic challenge due to its non-specific clinical presentation. Among its varied manifestations, gastrointestinal bleeding as an initial presentation is exceedingly rare. This case report aims to highlight the diagnostic difficulties, treatment modalities, and clinical outcomes in a patient with GITB presenting as gastrointestinal bleeding.</p><p><b><i>Case Description:</i></b> This is a case of a 24/M, no known comorbidities, immunocompetent host, no maintenance medications who came in for sudden onset of hematochezia with progressive drop in hemoglobin levels. Work up revealed a circumferential wall thickening involving the ileocecal region. Bleeding from mass was difficult to control despite medications and colonoscopy attempts hence patient underwent exploratory Laparotomy, right segmental colectomy. Histopathology results revealed caseating chronic granulomatous inflammation and lymph nodes showing caseating granulomas. Patient was then started on anti-Kochs treatment with note of improvement of symptoms post treatment.</p><p><b><i>Discussion:</i></b> Gastrointestinal tuberculosis (GITB) which can mimic various other gastrointestinal disorders makes the diagnosis challenging, often leading to delayed or missed identification. Gastrointestinal bleeding as an initial presentation is an unusual but documented occurrence. The treatment of gastrointestinal tuberculosis presenting as bleeding involves a comprehensive approach that typically combines anti-tubercular therapy (ATT) with surgical intervention. A high index of suspicion accompanied by promptly intervention are key to improving patient care.</p><p><b>PP-02-120</b></p><p><b>Small Intestinal Burkitt's Lymphoma presenting as an Acute Abdomen secondary to Ileo-Colic Intussusception</b></p><p><b>Christine Velasquez</b>, Beatrice Delynn Go and Gerardo Pedregosa</p><p><i>Makati Medical Center, Makati, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Primary gastrointestinal lymphomas are rare, comprising 1–4% of gastrointestinal malignancies, with Burkitt’s lymphoma accounting for 0.3–1.3% of non-Hodgkin’s lymphomas. Typically affecting the ileum in children and adolescents, Burkitt’s lymphoma in adults can cause ileocolic intussusception. This report presents a rare case of ileocolic intussusception caused by small intestinal Burkitt’s lymphoma.</p><p><b><i>Case description:</i></b> A 28-year-old male presented with progressive colicky pain in the right lower quadrant for 4 days, accompanied by nausea and bloatedness. Medical history was unremarkable. Physical examination revealed a distended abdomen, hypoactive bowel sounds, tympanitic percussion, and direct and rebound tenderness in the right lower quadrant with guarding. Laboratory tests revealed leukocytosis, and CT scan identified a 3.5x4.2x3.6 cm mass at the ileocecal junction, indicating ileocolic intussusception The patient underwent a right hemicolectomy and ileo-transverse colostomy with end-to-side anastomosis. Intraoperative findings revealed a 5 cm intussuscepted ileum in the cecum, a 4x4x3 cm ileocecal valve lead point, and a 1.5 cm tumor. Biopsy was consistent with Burkitt’s lymphoma. The patient received chemotherapy with rituximab, etoposide, doxorubicin, vincristine, prednisone, cyclophosphamide, and filgrastim for 6 cycles. Follow-up CT scans and colonoscopy showed no new masses and normal colonic mucosa.</p><p><b><i>Discussion:</i></b> Burkitt’s lymphoma, linked to previous malabsorption syndromes, IBD, and immunosuppression, presents with nonspecific symptoms, such as abdominal pain. Diagnosis involves CT scans and laparotomy. Biopsy shows CD20 positivity and atypical lymphoid proliferation. Treatment includes surgical resection and chemotherapy. Prognosis varies by age and disease site. The survival rate is approximately 60%. More research on adult cases is recommended.</p><p><b>PP-02-121</b></p><p><b>Controlling nutritional status score as predictive marker in completing adjuvant capecitabine for biliary tract cancer</b></p><p><b>Sung Hoon Chang</b>, Jun Yeol Kim, Yong Soo Song, Tae Seung Lee, Jin Ho Choi, Woo Hyun Paik, Sang Hyub Lee, Ji Kon Ryu and In Rae Cho</p><p><i>Seoul National University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Adjuvant capecitabine has demonstrated survival benefit for patients with resected biliary tract cancer (BTC). However, about one-third of clinical trial population discontinued treatment due to toxicity. Previous studies have found that nutritional indices such as the Geriatric Nutritional Risk Index (GNRI) and the Controlling Nutritional Status (CONUT) score are related to chemotherapy tolerance. This study aims to assess whether nutritional indices can predict completion of adjuvant capecitabine and prognosis.</p><p><b><i>Materials and Methods:</i></b> We retrospectively investigated 61 BTC patients treated with adjuvant capecitabine from June 2019 to January 2023. Patients were categorized into two groups: those who completed adjuvant capecitabine and did not. Pre-treatment nutritional indices including GNRI, CONUT score, platelet-to-lymphocyte ratio, and neutrophil-to-lymphocyte ratio were analyzed and compared between the groups. Overall survival (OS), recurrence-free survival (RFS) and treatment-related adverse events (TRAEs) were also investigated.</p><p><b><i>Results:</i></b> Of 61 patients, 44 (72.1%) completed adjuvant capecitabine. Among the various nutritional indices, the CONUT score was significantly different between completion and incompletion groups (2 vs. 3; p=0.033). CONUT score ≤2 was associated with higher completion rates of adjuvant capecitabine (83.9 % vs. 55.6 %; p=0.038) and higher relative dose intensity (90% vs. 80%, p=0.044). Patients who completed adjuvant chemotherapy experienced fewer TRAEs (61.0% vs 94.1%; p=0.027) and tended to show better 3-year OS (37% vs. 18%; p=0.089) and RFS rates (22% vs. 18%; p=0.51).</p><p><b><i>Conclusion:</i></b> The CONUT score is helpful for predicting the completion of adjuvant capecitabine. It can aid in selecting appropriate patients for adjuvant chemotherapy and predicting prognosis.</p><p><b>PP-02-122</b></p><p><b>New chemical drugs MTM16 relieves cholesterol stones by regulating cholesterol metabolism</b></p><p><b>博士 Yirong Chi</b></p><p><i>Department Of Gastroenterology And Hepatology, Tianjin Medical University General Hospital, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Gallbladder stones are a common disease worldwide, characterized by a homeostatic imbalance of cholesterol, bile acids, and other components of bile. The novel chemical CPLT was found to have lipid-lowering effects in preliminary experiments, so we hypothesized that it might have a mitigating effect on gallbladder cholesterol stone formation. The aim of this study was to investigate the therapeutic effects and potential mechanisms of CPLT on cholesterol stones.</p><p><b><i>Methods:</i></b> C57BL/6J mice were randomly divided into 3 groups: the NC group (normal diet, equal volume of 1% DMSO), the LD group (stone-forming diet group, equal volume of 1% DMSO), and the CP group (LD diet, 30 mg/kg/d CPLT), which were fed and gavaged for 4 weeks. Lipid profiles in gallbladder bile were measured and cholesterol saturation index (CSI) was calculated. Metabolomic analysis of bile acid species in liver and feces was performed. Serum was taken for analysis of liver function and lipids. Gallbladder and liver tissues were taken for HE staining. The expression of cholesterol metabolism and transport genes in liver and ileum was detected.</p><p><b><i>Results:</i></b> CPLT reduced the gallstone score as well as CSI in mice, and led to a decrease in cholesterol concentration in gallbladder and lipid concentration in serum.CPLT improved hepatic fat vacuoles, and the thickness and structure of the gallbladder wall, etc. in the CP group improved compared with that in the LD group. The real-time PCR and Western blot assays revealed that ABCG5,ABCG8 and upstream LXRα were down-regulated in the liver by CPLT.</p><p><b>PP-02-123</b></p><p><b>The bleeding pancreas: case report of pancreatic carcinoma with contained pseudoaneurysm rupture status post embolisation</b></p><p><b>Kunhan Chiam</b></p><p><i>Tan Tock Seng Hospital, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pancreatic pseudoaneurysm is a rare vascular complication of pancreatitis resulting from erosion of the peripancreatic artery. It usually involves the splenic artery, gastroduodenal, pancreaticoduodenal, superior mesenteric artery. Bleeding can occur in the pseudocyst itself and even into surrounding organs. Management is embolization or surgical intervention. We report a patient with pancreatic carcinoma with a pseudoaneurysm.</p><p><b><i>Case Description and Discussion:</i></b> A 65 year old female presented with 3 months of epigastric pain with an epigastric mass on examination. Computed tomography scan showed a large 9.8cm x 8.2cm head of pancreas cystic-solid mass. Within the mass there was a 1.9cm lobulated area of focus with arterial phase enhancement and persistent hyperdensity in subsequent phases consistent with a pseudoaneurysm. Another 1.6cm segment 6 hepatic lesion seen likely metastasis. She underwent a liver biopsy of the S6 hepatic lesion which showed poorly differentiated carcinoma with neuroendocrine differentiation (positive for CK7, PAX8, Synaptophysin, negative for WT1, Ki67 stains 70-80% of the tumour cells. She was started on chemotherapy with Etoposide and Carboplatin.</p><p><b><i>Investigation:</i></b> Hb 11.2 g/dL, Bil 7umol/L, AST 17 ALT 11 ALP 80 CA19-9 363 U/ml (Insert initial CT here) 2 months later, she had worsening abdominal pain with per-rectal bleeding and repeat CT showed enlargement of the pseudoaneurysm to 4.7cm with contained rupture. Hb 7.1 g/dL (Insert CT here)</p><p>She underwent radiologically guided embolization of the pseudoaneurysm (Insert below and after images here with description)</p><p>Patient’s symptoms improved and she was continue on chemotherapy and planned for repeat CT two weeks thereafter.</p><p><b>PP-02-124</b></p><p><b>Hemobilia from an intraductal papillary neoplasm of the bile duct presenting as recurrent melena</b></p><p><b>Jemimah Andrea Fajardo</b> and Edgardo Bondoc and Evan Ong</p><p><i>St. Luke's Medical Center Quezon City, Quezon, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>Hemobilia, a bleeding event from or into the biliary tract, still remains to be an uncommon cause of gastrointestinal bleeding worldwide. Its clinical presentation and etiology may vary which is often a challenge as it can lead to potential lethal sequelae if not properly recognized and treated. Hence, a systematic approach is prudent to locate and possibly rule out rare malignancies.</p><p>A 70-yo-Filipino-female, known hypertensive-diabetic, cervical, colon carcinoma in remission complained of 4-month history of recurrent melena underwent Esophagogastroduodenoscopy(EGD), Magnetic-resonance-cholangiopancreatography(MRCP), Enteroscopy two-months prior revealed chronic gastritis, gastric ulcers FCIII.</p><p>Melena recurred hence repeat EGD, push enteroscopy revealed Hemobilia. To control bleeding, underwent Celio-Mesenteric Angiogram subsequent Particle Embolization of Distal Right Hepatic Arteries, Distal Middle Hepatic Artery.</p><p>Dynamic CT scan of the liver and abdomen clinched the location it revealed intraluminal heterogenous foci, hyperdensities in the dilated common bile duct, intrahepatic bile ducts, nodular enhancing focus. As to exhaust the investigation, a Gastrointestinal bleed scintigraphy and direct visual Cholangioscopy was done to go beyond across the ducts in an odyssey to reveal a lobulated polypoid lesion, villous surface at the main right intrahepatic duct. Biopsy revealed intraductal papillary neoplasm, low grade intraepithelial neoplasia concluded the quest.</p><p>Referred to Surgery, final biopsy signed out as Intraductal Papillary Mucinous Neoplasm Of The Bile Duct(IPNB), focal high grade dysplasia. Patient was discharged, given palliative care.</p><p>IPNB remains to be a rare cause of UGIB.</p><p>A prompt diagnosis through a systematic approach through various advanced diagnostic procedures is necessary in preventing disease progression, and mortality.</p><p><b>PP-02-126</b></p><p><b>Effect of diabetes and insulin resistance on treatment outcomes in patients with pancreatic cancer chemotherapy</b></p><p>NAM HEE Kim and <b>Hong Joo Kim</b></p><p><i>Sungkyunkwan University Kangbuk Samsung Hospital, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We investigated whether diabetes mellitus (DM) and insulin resistance have any association with survival of patients with pancreatic cancer (PC) undergoing chemotherapy.</p><p><b><i>Materials and methods:</i></b> From March 2013 to March 2023, 265 patients who were pathologically confirmed to have PC at our hospital, received palliative, neoadjuvant, or adjuvant chemotherapy, and had a follow-up period of more than 6 months were enrolled in the study.</p><p><b><i>Results:</i></b> The overall survival of DM patients was significantly shorter than that of patients without DM (median survival for DM and non-DM patients was 7 and 12 months, respectively, p=0.038 by log-rank comparison). The overall survival of high fasting insulin group (defined as fasting insulin ≥ 10 uIU/ml) was significantly shorter than that of low fasting insulin group (median survival for high fasting insulin and low fasting insulin group was 5 and 17 months, respectively, p <0.01 by log-rank comparison). The overall survival of high HOMA-IR group (defined as HOMA-IR >4%) was significantly shorter than that of low HOMA-IR group (median survival for high HOMA-IR and low HOMA-IR group was 8 and 26 months, respectively, p <0.01 by log-rank comparison). Independent prognostic factors in pancreatic cancer patients receiving chemotherapy according to Cox regression analyses were high HOMA-IR group, NCCN stage, and initial RECIST.</p><p><b><i>Conclusions:</i></b> In pancreatic cancer patients receiving chemotherapy, the presence of diabetes and insulin resistance appear to be one of the factors that determine treatment outcomes.</p><p><b>PP-02-127</b></p><p><b>Neutrophil to Lymphocyte Ratio Predicts Infections in Acute Pancreatitis</b></p><p><b>Gauri Kumbhar</b>, Reuben Thomas Kurien and Sudipta Dhar Chowdhury</p><p><i>Christian Medical College, Vellore, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Differentiating infections from sterile inflammation is crucial in early Acute Pancreatitis (AP) management. This study aimed to analyse the profile, the impact of infections in AP and to assess the capability of the Neutrophil-to-Lymphocyte Ratio (NLR) to discriminate between sterile inflammation and infections in the first week of illness.</p><p><b><i>Materials and Methods:</i></b> Patients with AP developing a fever were included. Infections were categorized as infected pancreatic necrosis(IPN) and extra-pancreatic infections(EPI). Microbiological profiles and serious adverse events (SAE-in hospital mortality or discharge in critical state) were analysed. The ability of NLR and procalcitonin to discriminate infection from sterile inflammation in the first week was assessed.</p><p><b><i>Results:</i></b> Of 505 patients with AP, 150 developed fever. 48 (32%) had sterile inflammation, while 102 (68%)had infections. Within infections, 44 had IPN, 68 had EPI, and 10 had both. Of 54 patients with culture-positive infections, 36(66.6%) had infections by multidrug-resistant (MDR) organisms. Fungal isolates were identified in 5 patients. Patients with infections had higher SAE incidence (21.6% vs. 4.2%,p = 0.007) than those with sterile inflammation. The SAE incidence was higher among patients with MDR infections than those without MDR (37.5% vs. 9.3%,p=0.006). Most patients (n = 98, 65.3%) developed fever during the first week of illness. NLR demonstrated good accuracy in discriminating infections from sterile inflammation in the first week (AUC 0.70,p= 0.001), outperforming procalcitonin (AUC 0.54,p = 0.58).</p><p><b>PP-02-128</b></p><p><b>Immune phenotype by AI-powered spatial analysis of tumor-infiltrating lymphocytes predicts prognosis in resected pancreatic cancer</b></p><p><b>Jong Kyun Lee</b><sup>1</sup>, Hyemin Kim<sup>1</sup>, Young Hoon Choi<sup>1</sup>, Yoo Joo Lim<sup>2</sup>, Kyu Taek Lee<sup>1</sup>, Kwang Hyuck Lee<sup>1</sup> and Joo Kyung Park<sup>1</sup></p><p><sup>1</sup><i>Department Of Medicine, Samsung Medical Center, Sungkyunkwan University School Of Medicine, Seoul, South Korea;</i> <sup>2</sup><i>Lunit Inc, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Tumor-infiltrating lymphocyte(TIL) have been implicated as prognostic biomarkers across various malignancies. However, their prognostic relevance in resected pancreatic ductal adenocarcinoma(PDAC) remains elusive. This study evaluates the utility of artificial intelligence(AI)-powered spatial mapping of TIL density in PDAC and its correlation with clinical outcomes.</p><p><b><i>Materials and Methods:</i></b> A total of 304 PDAC patients who underwent upfront surgery with curative intention enrolled in this study, and their R0 resected specimens of whole slide images stained with hematoxylin and eosin(H&E) were analyzed. We utilized Lunit SCOPE IO, an AI-powered WSI analyzer, for spatial TIL quantification, segmentation of tumor and stroma, and immune phenotype(IP) classification as immune inflamed phenotype(IIP), immune excluded phenotype(IEP), or immune desert phenotype(IDP).</p><p><b><i>Results:</i></b> The median intratumoral TIL(iTIL) density and stromal TIL (sTIL) density were 100.64/mm2 (Interquartile range[IQR], 53.25–121.39) and 734.88/mm2 ((IQR 443.10–911.16), respectively. The TILs in the tumor microenvironment were predominantly concentrated in the stroma, and both iTIL and sTIL were significantly higher in IIP compared to IEP and IDP (P< 0.001). IIP was favorably related to overall survival(OS) and recurrence-free survival(RFS), and exceptionally high iTIL density was associated with longer OS (P=0.004) and RFS (P=0.021). This prognostic significance persisted in multivariable analysis, including other clinicopathologic factors.</p><p><b><i>Conclusion:</i></b> Notably, a higher density of iTILs was associated with improved prognostic outcomes. AI has markedly condensed the labor-intensive process of TIL assessment, potentially rendering TIL more feasible and practical in clinical application. More importantly, immunophenotype can be one of the most important biomarkers to predict OS and RFS in resected PDACs.</p><p><b>PP-02-129</b></p><p><b>Triceps Skinfold Thickness and Serum Albumin Changes in Pancreatic Cancer Chemotherapy: Sex-Based Prognostic Insights</b></p><p><b>Jong Kyun Lee</b>, Young Hoon Choi, Kyu Taek Lee, Kwang Hyuck Lee and Joo Kyung Park</p><p><i>Department Of Medicine, Samsung Medical Center, Sungkyunkwan University School Of Medicine, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Cachexia is prevalent in pancreatic cancer patients. We explored the association between triceps skinfold thickness(TSF), indicative of body fat, and serum albumin levels, reflecting nutritional status, with overall survival(OS) in pancreatic cancer patients.</p><p><b><i>Materials and Methods:</i></b> A prospective cohort of 353 pancreatic cancer patients receiving chemotherapy was analyzed. TSF and serum albumin were measured at baseline and every three weeks during follow-up. We performed a landmark analysis of OS six months after the diagnosis of pancreatic cancer and assessed the factors influencing OS. Sex-specific survival analysis was conducted, culminating in the construction of nomograms for risk stratification.</p><p><b><i>Results:</i></b> Two hundred ninety-two patients survived over six months, with a median follow-up of 40.9 months. Changes in TSF, albumin, and protein levels over six months and initial albumin levels were independently associated with OS. Cancer stage, response to chemotherapy, and six-month serum CA19-9 levels were also independent factors for OS. Sex-stratified analysis revealed sex-specific prognostic factors: TSF(mm) changes over six months≤ 0 were associated with reduced OS (hazard ratio[HR], 1.84; 95% confidence interval[CI], 1.14–2.95) in males; initial albumin levels≤ 3.5g/dl (HR, 5.73; 95% CI, 2.48–13.21) and albumin changes over six months≤ -0.7g/dl (HR, 3.03; 95% CI, 1.51–6.07) were associated with reduced OS in females. Patients were categorized into low-, medium-, and high-risk groups per sex using nomogram scores, with increased risk levels linked to higher mortality risk.</p><p><b><i>Conclusion:</i></b> This study highlights the significant association of TSF and serum albumin levels with OS in pancreatic cancer patients undergoing chemotherapy, differing by sex.</p><p><b>PP-02-130</b></p><p><b>Antibiogram and Bacterial Profile Susceptibility in Patients with Suspected Cholangitis Undergoing Biliary Drainage</b></p><p><b>Abhay Mahajan</b>, C Ganesh Pai, Ganesh Bhat, Shiran Shetty, Balaji Musunuri and Athish Shetty</p><p><i>KMC, Manipal, Udupi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The present study aims to identify the most commonly grown bacteria, their antibiotic susceptibility, and the risk factors for bacterial colonization in bile collected during biliary intervention in patients with different causes of biliary obstruction.</p><p><b><i>Methods:</i></b> This prospective study was conducted at tertiary care of coastal Karnataka. Patients aged>18 and with suspected cholangitis undergoing ERCP and percutaneous drainage were included in the study. Bile specimens were collected and transported to the microbiology lab in blood culture bottles using an aerobic transport system.</p><p><b><i>Results:</i></b> A total of 50 patients were enrolled in the study with a mean age of 48.39±13.5, with most being male individuals [n, 42 (84%)]. Of 50, 38 were culture positive. The most frequently encountered organisms were Gram-negative bacteria, including Escherichia coli [n, 12 (28.2%)], followed by Klebsiella [n,7 (17.3%)]. Among the antibiotics, amoxicillin-clavulinic acid [n,7 (18.4%)] and ceftriaxone [n,5 (13.1)] were found to be more resistant. No significant correlations were found between the type of bacterial colonization and antibiotic sensitivity to a type of obstruction prior to biliary interventions. However, a history of prior antibiotic use was found to be a significant factor associated with the risk of antibiotic resistance.</p><p><b><i>Conclusions:</i></b> The present study recommends cautious antibiotic selection in cholangitis management, given the predominance of Gram-negative bacteria such as Escherichia coli and Klebsiella in bile cultures and observed resistance to amoxicillin-clavulanic acid and ceftriaxone. Further, the present study recommends developing local antibiotic treatment regimens concerning their antibiotic susceptibility.</p><p><b>PP-02-131</b></p><p><b>Shedding Light on a Rare Encounter: Case Report of Pancreatic Neuroendocrine Tumor in the Philippines</b></p><p><b>Natasha Bernadine Mapa</b> and Roel Galang</p><p><i>Makati Medical Center, Makati City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pancreatic neuroendocrine tumors (pNETs) represent a rare subset of neoplasms arising from the endocrine cells of the pancreas. These tumors are characterized by their unique biological behavior, diverse clinical manifestations and potential for hormone production.</p><p><b><i>Case description:</i></b> This is a case of 39/M, no known comorbidities who came in for hematuria and back pain. Work up revealed a pancreatic mass with multiple liver lesions to consider metastasis. Tumor markers requested were unremarkable. Full PET CT showed a DOTATATE-avid mass in the pancreas with pituitary, neck, peripancreatic and axillary lymph nodes, liver foci, adrenal glands, thyroid gland, and the most intensely DOTATE-avid lesion was seen in the pancreas. Endoscopic Ultrasound with biopsy of pancreatic mass was done which revealed a Pancreatic Neuroendocrine Tumor Grade 2. Patient was given Lutetium-177 as treatment and after two doses, noted stable pancreatic mass with slight decrease in uptake in some hepatic lesions.</p><p><b><i>Discussion:</i></b> The treatment of a pancreatic neuroendocrine tumor (pNET) is complex and depend on several factors, including the tumor's size, grade, stage, location, and the presence of metastasis. Because of the indolent course and nonspecific presentation of the disease, many patients are diagnosed with locally advanced disease at the onset- limiting treatment options. A high index of suspicion is necessary to diagnose and treat this disease early to prevent further complications.</p><p><b>PP-02-132</b></p><p><b>Utility of D-dimer, antithrombin-III, protein c in prediction of severity and prognosis of acute pancreatitis</b></p><p><b>NS Ravichandra</b> and Vineet Chaudary and Sumaswi Angadi and Bhushan Chopade and Sukanya Bhrugumalla</p><p><i>Nizams Institute Of Medical Sceiences, Hyderabad, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The study was conducted to assess the predictive value of D-dimer, antithrombin III, protein C, and other coagulation parameters in the severity and prognosis of acute pancreatitis.</p><p><b><i>Materials and Methods:</i></b> This is a single-center, prospective observational study of patients admitted with acute Pancreatitis (AP) in a tertiary healthcare center in south India between January 2018 and January 2019. Platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen, antithrombin III (AT III), Protein C, and D-dimer were measured in plasma on day 0, and day 3 of admission. The severity of AP defined according to revised Atlanta classification. Outcomes like length of hospital stay, organ failure, and in-hospital mortality were analyzed.</p><p><b><i>Results:</i></b> 61 patients included; 15 (25%) mild, 24(39%) moderately severe and 22 (36%) patients severe acute pancreatitis, out of which 9 (15%) patients died during the hospital stay. PT, D- dimer and fibrinogen levels were significantly higher whereas platelet count, protein C, and AT III were significantly lower among severe pancreatitis and non-survivors. Fibrinogen levels, TT, and APTT were not statistically different between survivors and survivors. Protein C, and AT III on day 3 at cut-off ≤72% and ≤82% respectively were better predictors of severe acute pancreatitis with an AUROC of 0.886, and 0.877 respectively. D- Dimer on day 0 at cut-off ≥ 10μg/ml was a better predictor of mortality with an AUROC of 0.881.</p><p><b><i>Conclusion:</i></b> Coagulation parameters in the early phase can be utilized to predict the severity and prognosis of acute pancreatitis.</p><p><b>PP-02-133</b></p><p><b>Basic and clinical significance of epithelial membrane protein 1 (EMP1) in pancreatic ductal adenocarcinoma</b></p><p><b>Akihisa Ohno</b>, Nao Fujimori, Takahiro Ueda, Shotaro Kakehashi, Kazuhide Matsumoto, Masatoshi Murakami, Katsuhito Teramatsu, Keijiro Ueda and Yoshihiro Ogawa</p><p><i>Kyushu University, Fukuoka, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The expression of Epithelial Membrane Protein 1 (EMP1) has been reported in cases of various cancer types, with EMP1 identified as a potential prognostic biomarker in patients with pancreatic ductal adenocarcinoma (PDAC). However, its role in PDAC remains unknown. Therefore, we aimed to investigate the importance of EMP1 in PDAC.</p><p><b><i>Materials and Methods:</i></b> Mouse cancer cell lines (KrasLSL-G12D; Trp53LSL-R172H; Pdx1-Cre: KPC mice), human cell lines (Panc-1 and MIAPaCa-2) and patient-derived organoids (PDOs) from patients with PDAC were used to investigate the functional role of EMP1. Bioinformatics analyses were performed using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus.</p><p><b><i>Result:</i></b> We generated EMP1 knockout (KO) and overexpression cell lines in vitro and found high proliferative and metastatic potential drug resistance in the group with high EMP1 expression among all three models. In vivo, we found EMP1 was essential for the proliferation and metastasis of PDAC in mouse models using intrapancreatic and intrasplenic injection. Eleven PDOs were derived from patients with PDAC. EMP1 expression was significantly higher in the liver metastasis group than in the locally advanced groups. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed drug resistance in the group with high EMP1 expression. Mechanistically, RNA-sequencing of the EMP1 KO mouse model and analysis of the TCGA dataset revealed that K-RAS signaling, and epithelial-mesenchymal transition (EMT) were closely correlated with EMP1.</p><p><b><i>Conclusion:</i></b> EMP-1 is associated with the proliferative and metastatic potential of PDAC through the K-RAS pathway and EMT and may be a future therapeutic target.</p><p><b>PP-02-134</b></p><p><b>Systematic Review of Research Progress on Borderline Resectable Pancreatic Cancer using Bibliometric and Visualized Analysis</b></p><p><b>Jae Keun Park</b><sup>1</sup> and Ji Woong Hwang<sup>2</sup></p><p><sup>1</sup><i>Hallym University Kangnam Sacred Heart Hospital, Seoul, South Korea;</i> <sup>2</sup><i>Chung-Ang University Gwangmyeong Hospital, Chung-Ang University College of Medicine, Gwangmyeong, Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Borderline resectable pancreatic cancer, an intermediate stage between a completely resectable state and an unresectable state, requires a multidisciplinary treatment approach. This study aimed to elucidate the main characteristics and recent research trends regarding borderline resectable pancreatic cancer to gain further insights into them.</p><p><b><i>Materials and Methods:</i></b> Data from published papers about borderline resectable pancreatic cancer were collected from Web of Science (2014–2023) for the analysis. This study included 355 papers; data on major countries, publishing organizations, and keywords were collected and analyzed. Furthermore, R studio and VOSviewer were used for the qualitative and quantitative analyses of keywords.</p><p><b><i>Results:</i></b> Publication of papers on borderline resectable pancreatic cancer was observed to be increasing annually by 12.8%, with the United States and Japan being the main publishing countries. In 2014, keywords related to surgery and chemotherapy were dominant; however, a shift toward more integrative approaches, such as neoadjuvant therapy, was observed over time.</p><p><b><i>Conclusion:</i></b> This study demonstrates rapidly evolving trends and paradigm changes in the research and management of borderline resectable pancreatic cancer. Thus, the results of this study are expected to contribute to establishing future research strategies and improving patient treatment outcomes.</p><p><b>PP-02-135</b></p><p><b>Long term outcomes of splanchnic venous thrombosis in acute pancreatitis – A prospective cohort study</b></p><p>Sudipta Dhar Chowdhury, <b>Samanvith Patlori</b>, Betty Simon, Gauri Kumbhar, Reuben Thomas Kurien and Paul Deepak</p><p><i>Christian Medical College, Vellore, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Splanchnic venous thrombosis (SVT) is a serious complication of acute pancreatitis(AP). The natural history of SVT in AP is not well defined. Aim of this study aimed was assess the natural history of SVT in patients with AP.</p><p><b><i>Materials and Methods:</i></b> Patients with AP admitted between October 2018 to September 2023 were included. Inclusion criteria -1) First episode of AP, 2)Presence of SVT. Patients with chronic pancreatitis, recurrent AP, cirrhosis, malignancy and follow-up l< 6 months were excluded. Baseline data of patients, aetiology, severity, imaging, anticoagulation details were collected. Doppler ultrasound was performed at ≥6 months to assess for re-canalization.</p><p><b><i>Results:</i></b> 814 patients were admitted with AP during the study period. SVT was diagnosed in 92 patients. Of the 92 patients, 10 died, 9 were lost to follow up and 3 refused consent. 70 patients with SVT were included in the study. The mean age was 38.1(SD 12)years, 65(92.8%) were males. Predominant aetiology was alcohol(44 (62.8%) patients). 64(91.4%) had necrotizing pancreatitis. SVT was seen more frequently in retro-pancreatic splenic vein (47(67.1%)). 14(20%)patients received therapeutic anticoagulation. On follow up, complete recanalization was noted in 38(54.3%) and partial recanalization in 2(2.9%) patients. At univariate analysis on admission BISAP >2 was a risk factor for non-recanalization of SVT(p-0.007). Anticoagulation had no significant effect on recanalization rates in patients with SVT(64.3% with anticoagulation vs. 35.7% without anticoagulation, p=0.764).</p><p><b><i>Conclusion:</i></b> Majority of patients who develop SVT in the setting of AP spontaneously recanalize on follow up. Severity of pancreatitis predicts non recanalization of SVT.</p><p><b>PP-02-136</b></p><p><b>Efficacy of thoracic epidural versus intravenous analgesia for pain control and morbidity in acute pancreatitis</b></p><p><b>Pulkit Sondhi</b>, Neha Berry, Pradyut Bag, Vidhi Mahendru, Manav Wadhawan and Ajay Kumar</p><p><i>BLK Max Superspeciality Hospital, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Primary objective was to assess pain control and secondary objectives were to assess efficacy of epidural and IV analgesia on organ failure,morbidity and mortality.</p><p><b><i>Materials & methods:</i></b> Over a period of one year,all consecutive patients of AP with moderate to severe pain with numerical rating scale (NRS ≥4) were sequentially allocated into 2 groups-Epidural Patient controlled analgesia (PCA) (Ropivacaine + Fentanyl) and IV PCA (Fentanyl) + IV Paracetamol for 5 days.Morphine was used as rescue analgesia when NRS ≥4.</p><p><b><i>Results:</i></b> We enrolled 36 patients (18 in each group).Baseline characteristics were comparable between two groups.The mean number of days with adequate pain control was 3.28 ± 0.75 days in epidural vs 3.11 ± 0.76 days in IV group (p = 0.496).Adequate pain control was achieved at day 3 in both groups.9(50%) Patients in IV group required rescue analgesia vs 7 (38.9%) in epidural group and the cumulative dose of rescue analgesia required was 60 mg in epidural group vs 76 mg in the IV group (p = 0.83).Most commonly observed organ failure was acute lung injury in 6 (16.7%),3 in each group (p = 1.0).The resolution of SIRS at day 3 in comparison to baseline was better in epidural group as compared to IV group (70% vs 27.7%) (p = 0.089).There was no mortality in either group.</p><p><b><i>Conclusion:</i></b> Thoracic epidural PCA and IV PCA were equally efficacious in pain control in AP.Moreover,thoracic epidural analgesia was safe,feasible,required less rescue analgesia and showed an insignificant clinical trend towards early SIRS resolution.</p><p><b>PP-02-137</b></p><p><b>Purtscher-like retinopathy: A rare complication of acute on chronic pancreatitis</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Kavinda Bandara<sup>2</sup>, Chanika Gamage<sup>3</sup>, Pradeepa Siriwardene<sup>3</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgeyr, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>The University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>The National Eye Hospital, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Purtscher’s retinopathy is an occlusive micro-vasculopathy causing sudden onset visual loss. Similar retinal appearance is observed as a very rare complication of acute pancreatitis which is identified as Purtscher-like retinopathy(PLR). Here we present a patient with acute on chronic pancreatitis developed sudden unilateral blindness due to PLR.</p><p><b><i>Case Description:</i></b> A 15-year-old female with idiopathic acute on chronic pancreatitis who noticed a sudden onset loss of vision in the left eye and was found to have significantly diminished visual acuity. A dilated fundoscopic examination demonstrated pathognomonic Purtscher flecken, multiple retinal haemorrhages, cotton wool spots and macular oedema. A clinical diagnosis of Purtscher-like retinopathy was made in the setting of acute on chronic pancreatitis. Optical coherence tomography was used to support the diagnosis and to monitor response to therapy. She was treated with intravitreal bevacizumab and triamcinolone immediately upon diagnosis.Although she had significant improvement on fundoscopy, she only had slight improvement vision from 1/60 to 2/60. Optical coherence tomography showed poor prognostic factors like disrupted inner segment/outer segment junction and macular atrophy.</p><p><b><i>Discussion:</i></b> This is a rare vision threatening complication of acute pancreatitis often undiagnosed or underreported. Leuko-embolism and fat embolism casing retinal ischaemia are probable pathophysiological mechanisms. Given the variable prognosis with no evidence-based therapies available, she had a subjective improvement in visual acuity with administration of intravitreal steroids and observation with management of the acute episode of pancreatitis.Knowledge and awareness of this rare condition will enable its early detection and the search for newer therapies.</p><p><b>PP-02-138</b></p><p><b>A Recherche Entity: Pancreatic Tuberculosis In Non-Immunocompromised Patients: Case Reports And Review Of The Literature</b></p><p><b>Erika Johanna Tañada-Escanlar</b>, Frederick Dy and Estrellita Ruiz</p><p><i>UST Hospital, Manila, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Pancreatic tuberculosis is rare and an even rarer occurrence in immunocompetent patients masquerading as pancreatic malignancy. We present two cases of pancreatic TB in a young and middle age woman presenting with abdominal pain and weight loss with no risk factor for immunosuppression and we review the literature to describe the diverse clinical manifestations and diagnosis of this condition.</p><p><b><i>Case presentation:</i></b> A 22-year-old female presented with 1 month history of abdominal pain and weight loss and denied fever, night sweats, constitutional symptoms or tuberculosis contact. On examination, there was no palpable mass per abdomen with normal CA-19-9 (9 U/ml). Ultrasonography revealed 2.2 x 5.5 cm complex mass on pancreatic head, contrast-enhanced computed tomography (CT) of the abdomen revealed 4x5 cm pancreatic head mass with paraaortic lymphadenopathy. Another 43-year-old female with also no history of tuberculosis contact and immunosuppression presented with two months abdominal pain and weight loss. Her abdomen CT scan revealed a 8x3x5 cm hypoenhancing hypodense pancreatic head lesion. Two patients underwent EUS with FNA biopsy which showed chronic caseating granulomatous inflammation with microabscesses and their pancreatic tissue geneXpert were positive for mycobacterium tuberculosis. The patients were subsequently started on antitubercular medication and recovered well.</p><p><b><i>Conclusion:</i></b> Notwithstanding the fact that some case reports have been published, a high index of suspicion is still needed to diagnose pancreatic tuberculosis especially in immunocompetent patients. EUS-guided fine-needle aspiration is the diagnostic modality of choice and pancreatic geneXpert can be a useful tool for the diagnosis of pancreatic tuberculosis.</p><p><b>PP-02-139</b></p><p><b>Predictors of Pain Relapse after Ductal Interventions in Chronic Pancreatitis: A Machine Learning-Based Approach</b></p><p><b>Misbah Unnisa</b><sup>1</sup>, Abdul Rasheed<sup>1</sup>, Madhurya Reddy Chinthakuntla<sup>2</sup>, Rajesh Goud<sup>1</sup>, D. Nageshwar Reddy<sup>1</sup> and Rupjyoti Talukdar<sup>1</sup></p><p><sup>1</sup><i>Asian Institute Of Gastroenterology (AIG) Hospitals, Hyderabad, India;</i> <sup>2</sup><i>KU School of Medicine, Wichita, USA</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> While ductal interventions can improve pain in chronic pancreatitis (CP), their impact on disease progression remains unclear. We aimed to develop machine learning-based models to identify clinical parameters that predict pain recurrence after ductal interventions.</p><p><b><i>Materials and methods:</i></b> In this cross-sectional study, consecutive patients with documented CP were screened and enrolled between August 2022 and November 2023. Clinical, laboratory, imaging, and treatment data were recorded. 18-Preintervention independent-clinical predictors were included with dependent variable as pain relapse after initial improvement. The dataset was split into 80% training and 20% testing. Machine Learning(Python.3) algorithms namely DecisionTree, LogisticRegression, RandomForest, Support-Vector-Machine, CatBoost, and XGBoost were employed. Models performed evaluation metrics mainly AUROC, accuracy and Root Mean Squared Error(RMSE). SMOTE and simple imputer addressed data imbalance and missing values, respectively. Bootstrapping estimated the confidence interval.</p><p><b><i>Results:</i></b> 353 patients were recruited, and the final prediction model was based on 247 patients (Mean age 35.24±12.51 years;65.04% male) details before interventions. Most common etiology was idiopathic (66.39%). On the testing dataset, Logistic regression emerged as the top performer with an accuracy of 86% with AUROC of 0.904(95% CI: 0.798-0.98) and its RMSE was 0.374. CatBoost accuracy was 0.76 and AUROC of 0.754(95%:0.603-0.892) with RMSE was 0.489. Highlighted predictors were depression, long-standing diabetes, spontaneous pain, piercing, dragging and pressure nature of pain as consistently influential across models, underscoring their importance in predicting the outcome.</p><p><b><i>Conclusion:</i></b> Based on Logistic Regression model, we identified key predictors of pain relapse after ductal Interventions, that to be tested in large multicentre cohorts.</p><p><b>PP-02-140</b></p><p><b>Oxidative balance score and gallstones:Lesson from NHANES</b></p><p><b>Bo Wang</b><sup>1</sup>, Yongqiang Xiong<sup>1</sup>, Na Huang<sup>2</sup>, Jun Li<sup>2</sup> and Shu Zhang<sup>1,3</sup></p><p><sup>1</sup><i>Department of Geriatric General Surgery, The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;</i> <sup>2</sup><i>National & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;</i> <sup>3</sup><i>Experimental Teaching Center for Clinical Skills, The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Gallstones pose a significant burden on public health. The Oxidative Balance Score (OBS) integrates dietary and lifestyle factors to evaluate antioxidant exposure and oxidative stress. However, the relationship between OBS and gallstones is not well understood.</p><p><b><i>Methods:</i></b> To investigate the association between OBS and gallstones, we analyzed the data from National Health and Nutrition Examination Survey (NHANES) and employed a weighted multivariable logistic regression model, supplemented by subgroup analyses. Mendelian randomization (MR) was conducted to explore the causal relationships between OBS components and gallstones, using inverse variance weighting (IVW) as the primary analytical method. Sensitivity analyses were also performed.</p><p><b><i>Results:</i></b> The observational study found a significant inverse correlation between OBS and gallstones. Compared to the first quartile (Q1), the risk of gallstones was reduced by approximately 25% in the third quartile (Q3) (OR: 0.742, 95% CI 0.589-0.933, P = 0.011) and by about 31% in the fourth quartile (Q4) (OR: 0.693, 95% CI 0.549-0.873, P = 0.002). Subgroup analyses revealed that this association was stronger in individuals under 60 years, females, married individuals, and those with a college education or higher. MR analysis indicated a causal relationship between certain OBS components—namely vitamin B12, physical activity, BMI, and smoking—and the risk of gallstones, supported by sensitivity analyses.</p><p><b><i>Conclusion:</i></b> A high OBS is associated with a reduced risk of gallstones, particularly among individuals under 60 years, females, married individuals, and those with higher education levels. Key mediating factors include vitamin B12, physical activity, BMI, and smoking.</p><p><b>PP-02-141</b></p><p><b>The association between sarcopenia and gallstones: A cross-sectional study from NHANES</b></p><p><b>Bo Wang</b><sup>1</sup>, Qianxi Huang<sup>2</sup>, Yongqiang Xiong<sup>1</sup> and Shu Zhang<sup>1</sup></p><p><sup>1</sup><i>The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, China;</i> <sup>2</sup><i>Yangzhuang Community Health Center of Chang'an District, Xi’an, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Sarcopenia is an age-related condition characterized by a decline in muscle mass, strength, and function. However, its relationship with gallstones remains unclear.</p><p><b><i>Methods:</i></b> This cross-sectional study included 2,167 participants from the 2017-2018 National Health and Nutrition Examination Survey (NHANES). Sarcopenia was assessed using the sarcopenia index, and the association between sarcopenia and gallstones was evaluated using multivariable logistic regression models and restricted cubic spline (RCS) regression for dose-response curve analysis. Additionally, subgroup analyses and propensity score matching (PSM) were conducted to account for potential confounding factors.</p><p><b><i>Results:</i></b> We found a significant negative association between the sarcopenia index and the risk of gallstones (OR: 0.253, 95% CI: 0.132-0.471, P < 0.001). In Model 4, which integrated all covariates, sarcopenia was associated with approximately a 100% increased risk of gallstones compared to non-sarcopenia patients (OR: 1.995, 95% CI: 1.340-2.948, P < 0.001). The results from propensity score matching also confirmed sarcopenia as an independent risk factor for gallstones (OR: 1.982, 95% CI: 1.217-3.285, P = 0.007). Notably, this association was more pronounced in subgroups including females, non-Hispanic whites, married individuals, those with college education or higher, and in populations without diabetes, liver disease, or cancer.</p><p><b><i>Conclusion:</i></b> Our study suggests a significant association between sarcopenia and increased risk of gallstones among U.S. adults. This finding warrants validation through further large-scale prospective studies.</p><p><b>PP-02-142</b></p><p><b>rIL-22 alleviates severe acute pancreatitis and secondary multiple organ injury induced by cerulein in mice</b></p><p><b>Hongli Yang</b> and Weijia Sun and Feifei Zhou and Hongwei Xu</p><p><i>Provincial Hospital affiliated to Shandong First Medical University, Jinan, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Severe acute pancreatitis (SAP) is characterized by systemic inflammation that can lead to multiple organ failure and even death. However, effective treatment strategies are lacking. IL-22 has been shown to have anti-inflammatory effects. This study aims to investigate the therapeutic effects of rIL-22 on SAP and secondary multiple organ injury mice.</p><p><b><i>Materials and methods:</i></b> A mouse SAP model was constructed by intraperitoneal injection of cerulein and lipopolysaccharide, and then intraperitoneal injection of rIL-22. Peripheral blood, pancreas, liver, kidney, and lung tissues were collected. ELISA was performed to measure the levels of serum related indicators (AMY, LPS, ALT, AST, CRE, BUN, TNF-α, IL-6, and IL-1β). Histopathological changes were observed by HE staining. TUNEL assay was conducted to assess apoptotic cells. Immunofluorescence and Western blot were used to examine levels of Bax, caspase-3, and BCL2.</p><p><b><i>Results:</i></b> Compared to the NS group, HE staining of the SAP group revealed pancreatic acinar necrosis , disordered hepatocyte arrangement, alveolar wall swelling, and renal tubular epithelial cell necrosis. The levels of serum related indicators were elevated. Bax and caspase-3 levels were increased, while BCL2 was decreased. For rIL-22 group, HE staining showed reduced damage in the pancreas, liver, lung, and kidney tissues. The levels of serum related indicators were decreased. The expression of Bax and caspase-3 were decreased, and increased BCL2.</p><p><b><i>Conclusion:</i></b> rIL-22 can alleviate systemic inflammation and subsequent multiple organ injuries in SAP mice, providing a novel option for future SAP treatment.</p><p><b>PP-02-143</b></p><p><b>Single cell RNA sequencing revealed the role of Reg3 in acinar injury in acute pancreatitis</b></p><p><b>Pan Zheng</b>, Nianshuang Li and Yin Zhu</p><p><i>The first affiliated hospital of Nanchang University, Nanchang, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Acute pancreatitis is a common acute abdominal condition that can rapidly progress to severe states with high mortality rates. To investigate the pathogenesis of acute pancreatitis, we utilized single-cell transcriptomic sequencing to delve into the cellular landscape comprehensively and transcriptomic changes in cerulein-induced AP.</p><p><b><i>Material and methods:</i></b> Single-cell transcriptomic sequencing was employed to analyze cell-type compositions and their transcriptomic landscapes in mice with AP and healthy controls. Immunohistochemistry, immunofluorescence, qPCR, and western blotting were applied to validate significant differentially expressed genes among homotypic cells. Cerulein and pancreatic duct ligation induced AP in REG3A-/-, REG3B-/-, and REG3G-/- mice.</p><p><b><i>Results:</i></b> Following the onset of AP, the proportion and transcriptional profiles of pancreatic cells underwent a remarkable shift. As expected, inflammatory cells such as neutrophils and macrophages significantly increased in the AP group. Acinar cells during AP presented distinct transcriptomic expression profiles compared to normal acinar cells. Apart from the elevated expression of pancreatic enzyme genes, REG3A, REG3B, and REG3G genes also exhibited a significant increasing trend in acinar cells of AP. After validation of upregulated expressions of REG3A, REG3B, and REG3G in different AP mice models, we induced AP in REG3A, REG3B, and REG3G knockout mice and found a noticeable increase in the severity of murine AP, mainly manifested by extensive acinar necrosis.</p><p><b><i>Conclusion:</i></b> The pancreatic cells of mice with AP display distinct transcriptomic profiles and cell population proportions compared to healthy mice. The upregulation of REG3A, REG3B, and REG3G genes in acinar cells during AP plays a significant protective role.</p><p><b>PP-02-144</b></p><p><b>Comparison of de ritis, gamma-gt/albumin, alkaline-phospahatase/albumin ratios in obstructive jaundice patients with and without malignancy</b></p><p><b>Muhammad Naufal Zuhdi</b><sup>1</sup>, Numan AS Daud<sup>2,3</sup>, Susanto Hendra Kusuma<sup>2,3</sup>, Fardah Akil<sup>2,3</sup>, Muhammad Luthfi Parewangi<sup>2,3</sup>, Rini Rahmawarni Bachtiar<sup>2,3</sup> and Amelia Rifai<sup>2,3</sup></p><p><sup>1</sup><i>Internal Medicine Specialist Program, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>3</sup><i>Centre of Gastroenterology-Hepatology HAM Akil, Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The De Ritis ratio [Serum Glutamic Oxaloacetic Transaminase (SGOT)/ Serum Glutamic Pyruvic Transaminase (SGPT)] has shown that its elevated ratio is a risk factor in several solid tumors. Gamma GT/Albumin ratio (GAR) and Alkaline Phosphatase/Albumin ratio (APAR) also have been widely used as biomarker parameters to assess the prognosis of patients with solid tumors. This study aims to investigate the comparison of De Ritis ratio, GAR, and APAR in patients with obstructive jaundice with and without malignancy.</p><p><b><i>Materials and methods:</i></b> This study was conducted with a retrospective case control approach. The study population consisted of patients with obstructive jaundice in Wahidin Sudirohusodo Hospital which had undergone Endoscopic Retrograde Cholangiopancreatography (ERCP) procedure from January to June 2024. Malignancy diagnosed based on tumor markers and histopathology results. De Ritis Ratio, GAR and APAR were examined prior to the procedure.</p><p><b><i>Results:</i></b> We involved 62 subjects who had undergone ERCP for diagnosing obstructive jaundice due to either malignant with 25 (40%) subjects (56.2 ± 10.5 SD years old) or non-malignant with 37 (60%) subjects (45.8 ± 14.3 SD years old). Elevated enzymes concentration was identified in the entire of estimated parameters and decreased albumin serum concentration in both groups. These ratios consistently showed higher ratio in the malignant than in the non-malignant group. Statistical analysis estimated GAR value was significantly higher in obstructive jaundice caused by malignancy (Averaged GAR malignant = 123.3, 95% CI 85.8-160.8; p = 0.03, independent t-test).</p><p><b><i>Conclusion:</i></b> GAR is higher in obstructive jaundice because of malignancy.</p><p><b>PP-02-145</b></p><p><b>Predictive value of TyG index and VAT-related index for severity of hypertriglyceridemic pancreatitis</b></p><p><b>Rongjin Gu</b> and Yuerong Zhang</p><p><i>Department of Gastroenterology, University-Town Hospital of Chongqing Medical University, Chongqing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To investigate the relationship of triglyceride glucose (TyG) index and visceral adipose tissue-related(VAT) indices with hypertriglyceridemic pancreatitis (HTGP) and its predictive value for disease severity.</p><p><b><i>Materials and methods:</i></b> Retrospective study of a total of 281 patients with acute pancreatitis admitted to the University-Town Hospital from 2018 to 2023,and divided into two groups(HTGP and the non-HTGP group), each group was divided into the mild acute pancreatitis (MAP), moderately severe and severe acute pancreatitis (MS-SAP).Comparison of VAT-related indices(including Chinese visceral adiposity index (CVAI),visceral adiposity index (VAI),lipid accumulation product (LAP))and TyG index between two groups.The predictive value of TyG index and VAT-related indices on the severity of HTGP was evaluated using the ROC curve.</p><p><b><i>Results:</i></b> The VAT-related indices and TyG index in the HTGP group were higher than those in the non-HTGP group(p <0.001). The VAT-related indices and TyG index in the MS-SAP group were significantly higher than that in the MAP group(p <0.001).The ROC curve showed that the TyG index had the highest predictive efficacy for predicting HTGP disease severity, with a maximum cutoff value of 11.30 (AUC = 0.734,95% CI: 0.655 ~ 0.812, P <0.001).</p><p><b><i>Conclusion:</i></b> The VAT-related indices and TyG index were significantly higher in the HTGP group than in the non-HTGP group, with significant differences between the two groups, and the VAT-related indices and TyG index were positively correlated with the severity of HTGP. TyG index has the greatest value in predicting the severity of HTGP, which is better than other indexes.</p><p><b>PP-02-146</b></p><p><b>Hematemesis melena in duodenal tuberculosis: a case report</b></p><p><b>Hendra Asputra</b> and Hasan Maulahela</p><p><i>Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Primary duodenal tuberculosis is a very rare case with a prevalence of only 0.04% of all tuberculosis cases and 4.7% of all cases of intestinal tuberculosis. The lack of specificity of the symptoms makes duodenal tuberculosis difficult to diagnose which can lead to late treatment or complications.</p><p><b><i>Case Presentation:</i></b> A 61-year-old male patient came with complaints of vomiting blood and black stool since 3 weeks ago. The patient complained of heartburn, nausea and bloating since 6 months ago. During the last 1 month, the patient often vomit a few hours after eating. The patient has lost 10 kg in the last 6 months. There was no history of coughing up blood. The patient has a history of diabetes mellitus but was not compliant to treatment</p><p>The results of the physical examination showed anaemic conjunctiva and epigastric tenderness. Lab result showed hemoglobin 3.0. CT scan showed thickening of the wall of ascending duodenum with a dense mass of contrast. Esophagastroduodenoscopy resulted in lobulated, semicircular, fragile and bleeding mass in the duodenum which was suspected to be malignant. The results of the colonoscopy was grade 2 internal hemorrhoids. CEA tumor markers are within normal limits. Histopathological result was datia langhans cells and concluded chronic inflammation of tuberous granulomatose. The final diagnosis was tuberculosis of the ascending duodenum pars with bleeding.</p><p><b><i>Discussion:</i></b> Duodenal tuberculosis is rare and difficult to diagnose clinically. Endoscopy has an important role in the establishment of a proper diagnosis.</p><p><b>PP-02-147</b></p><p><b>Real-World Bleeding Risk of Anticoagulant and NSAID Combotherapy Versus Anticoagulant Monotherapy</b></p><p><b>Jae Myung Cha</b> and Moonhyung Lee</p><p><i>Kyung Hee University Hospital At Gangdong, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The incidence of acute gastrointestinal bleeding (GIB) increases with the utilization of anticoagulants and nonsteroidal anti-inflammatory drugs (NSAIDs). This study aimed to compare the risk of GIB between anticoagulant and NSAID combo therapy and anticoagulant monotherapy in real-world practice.</p><p><b><i>Materials and methods:</i></b> We investigated the relative risk of GIB in individuals newly prescribed anticoagulant and NSAID or anticoagulant monotherapy at three hospitals using ‘Common Data Model’. Cox proportional hazard models and Kaplan-Meier estimation were employed for risk comparison after propensity score matching.</p><p><b><i>Results:</i></b> A comprehensive analysis of 2,951 matched pairs showed that anticoagulant and NSAID combo-users exhibited a significantly higher risk of GIB compared to anticoagulant mono-users (hazard ratio [HR]=1.66; 95% confidence interval [CI]: 1.30–2.12; p<0.001). The risk of GIB associated with the combo-therapy of anticoagulant and NSAID was also significantly higher than that of anticoagulant monotherapy in elderly patients >65 years (HR=1.53; 95% CI: 1.15–2.03; p=0.003) and >75 years (HR=1.89; 95% CI: 1.23–2.90; p=0.003). We also found that the risk of GIB was significantly higher in the anticoagulant and NSAID combo-users than that of anticoagulant mono-users in both male (p=0.016) and female cohorts (p=0.010).</p><p><b><i>Conclusion:</i></b> The risk of GIB is significantly higher in anticoagulant and NSAID combo-users than anticoagulant mono-users. In addition, the risk of GIB associated with the combotherapy of anticoagulant and NSAID was much higher in elderly individuals >75 years. Therefore, physicians should pay more attention to the risk of GIB when they use anticoagulants and NSAIDs.</p><p><b>PP-02-148</b></p><p><b>Abnormal gastroesophageal flap valve: a predictor of recurrent variceal hemorrhage</b></p><p><b>Jay Chudasama</b></p><p><i>Topiwala National Medical College, Mumbai, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Aim:</i></b> To investigate the relationship between GEFV and esophageal variceal bleeding episodes.</p><p><b><i>Methods:</i></b> In this cross-sectional, retrospective study, 300 consecutive patients with esophageal varices and a documented GEFV during esophagogastroduodenoscopy were included. Patients were divided into two groups according to: the Hill’s grade of flap valve (grade 1,2- normal and grade 3,4- abnormal), size of varices - large (>5 mm) and small (<5 mm) and the number of bleeding episodes into: Group A with ≤ 1 and Group B with ≥ 2 bleeding episodes. We compared GEFV and various other factors to the number of variceal bleeding episodes.</p><p><b><i>Results:</i></b> 224 patients (74.60%) had a normal and 76 (25.40%) had an abnormal GEFV. Clinical variables were statistically significant in the abnormal GEFV group (P<0.0.5). Propensity score matching was done to reduce the significant differences in the clinical background at baseline between the 2 groups. 152 patients (76 in each group) were analysed after propensity score matching. A significant difference between the two groups disappeared except for number of bleeding episodes Binary logistic Cox regression analysis was applied using the clinical variables to assess their role in predicting recurrent variceal bleeding. On univariate analysis, abnormal GEFV and large varices were significantly associated with recurrent esophageal variceal bleed (P =0.001). On Multivariate analysis, abnormal GEFV (OR 7.25, P = 0.001) and large varices (OR 5.70, P = 0.001) were independent predictors for recurrent esophageal variceal bleeding</p><p><b><i>Conclusion:</i></b> Abnormal GEFV and large varices are independent risk factors for recurrent esophageal variceal haemorrhage.</p><p><b>PP-02-149</b></p><p><b>Validating the CHAMPS Score: A Novel and Reliable Prognostic Score of Non-Variceal Upper Gastrointestinal Bleeding</b></p><p><b>Thong Duy Vo</b> and Huong Tu Lam and Thang Dinh Nguyen and Hoang Huu Bui</p><p><i>University Medical Center Ho Chi Minh City, Ho Chi Minh, VietNam</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The CHAMPS score is a newly developed prognostic tool for assessing mortality risk in acute non-variceal upper gastrointestinal bleeding (NVUGIB). This study aims to externally validate the CHAMPS score and compare its predictive accuracy with established scoring systems.</p><p><b><i>Materials and methods:</i></b> A prospective cohort study was conducted involving 140 adult patients with NVUGIB admitted to the Department of Gastroenterology at Cho Ray Hospital, Ho Chi Minh City, from November 2022 to June 2023. The performance of the CHAMPS score in predicting in-hospital mortality and other clinical outcomes was assessed using area under the receiver operating characteristic (AUROC) curves. Comparative analyses were performed against the GBS, AIMS65, cRS, fRS and ABC score.</p><p><b><i>Results:</i></b> The CHAMPS score demonstrated superior performance in predicting in-hospital mortality with an AUROC of 0.89, significantly outperforming the GBS and AIMS65 scores (AUROC = 0.72 and 0.71, respectively; p < 0.05). The optimal CHAMPS score cut-off for predicting mortality was 3 points, with a sensitivity of 100% and specificity of 71.2%. Subgroup analysis confirmed its robust predictive capabilities for both bleeding-related and non-bleeding-related mortality (AUROC = 0.88 and 0.87, respectively). However, the CHAMPS score had limited predictive value for rebleeding and intervention (AUROC = 0.43 and 0.55, respectively).</p><p><b>PP-02-150</b></p><p><b>The Correlation Of Pre-Endoscopic Glasgow Blatchford Score With Post-Endoscopic Forrest Classification Of Peptic Ulcer Disease</b></p><p><b>Angela Gabasan</b>, Rolan-Ohmar Yumul and Jan Michael Kong</p><p><i>Victoriano Luna Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To validate the Glasgow Blatchford Scoring System by determining its correlation with the post-endoscopic Forrest Class in patients with upper gastrointestinal bleeding.</p><p><b><i>Materials and methods:</i></b> It employed a retrospective approach to validate pre-endoscopic Glasgow Blatchford Scoring system clinical outcomes of patients’ upper gastrointestinal bleeding using the Forrest classification. The medical records of 50 patients admitted at Victoriano Luna Medical Center from January 2018 until December 2019 were rigorously reviewed from records and recorded for the comparison and analysis of data. The chi-square test and Fisher’s exact test (two-sided) will be used to compare categorical variables.</p><p><b><i>Results:</i></b> A negative correlation was observed between Glasgow Blatchford Scoring System and Forrest Classification based on Chi Square and Fischer Exact Test (p .653 > .05). The Glasgow Blatchford score had a sensitivity of 80% with a specificity of 15%. The positive predictive value was at 19% and the negative predictive value of Glasgow Blatchford score was at 75%.</p><p><b><i>Conclusion:</i></b> This study revealed that the Glasgow Blatchford scores could be used as a tool for identifying patients who may be at high risk and may need more intensive intervention, and those patients who could be safely discharged. This conclusion was based on the results of this study that showed that this risk stratification tool had a high sensitivity for detecting high-risk ulcers, and a high negative predictive value indicating a low likelihood that patients assessed to have low-risk disease by GBS would have high-risk ulcers.</p><p><b>PP-02-151</b></p><p><b>Utilization of Hemostatic Powder Spray versus Standard Endoscopy for Malignancy-Related GI Bleeding: A Meta-Analysis</b></p><p><b>Laurence Laurel</b>, Ziggy Amores and Roel Galang</p><p><i>St. Luke's Medical Center-Global City, Taguig, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Managing tumoral bleeding is challenging for endoscopists. Traditional tools like clips and heat-probes often fail to control bleeding caused by mucosal invasion from tumors. Hemostatic powder sprays offer a novel solution by forming a mechanical barrier on the tumor surface to seal the bleeding site. However, data on their effectiveness in treating hemorrhagic gastrointestinal neoplasms is scarce. This study evaluates the efficacy of Hemostatic powder spray in patients with gastrointestinal bleeding related to malignancies.</p><p><b><i>Aims And Methods:</i></b> This study aimed to evaluate the effectiveness of Hemostatic powder spray compared to standard treatment in controlling gastrointestinal bleeding from malignant lesions. The primary outcome was rebleeding rates within 30 days, while the secondary outcome was overall mortality.</p><p>A comprehensive search of electronic databases was conducted up to April 2024, including only randomized controlled trials (RCTs) where Hemostatic powder spray was compared to conventional endoscopic techniques for managing gastrointestinal tumoral bleeding.</p><p><b><i>Results:</i></b> Three studies involving 162 patients were included. Among them, 83 patients received Hemostatic spray powder, while 79 received conventional hemostatic techniques. The analysis revealed that Hemostatic powder spray was associated with a lower incidence of re-bleeding beyond 30 days (odds ratio [OR] 0.36, 95% confidence interval [CI] 0.15-0.84), showing a significant advantage over standard endoscopy (p-value 0.01). No significant difference in overall mortality was observed between the two groups (OR 0.40, 95% CI 0.19-0.83, p-value 0.09).</p><p><b><i>Conclusion:</i></b> Hemosprays are effective in achieving hemostasis for gastrointestinal bleeding associated with malignancies. They can be considered a viable bridge to surgery and/or embolization.</p><p><b>PP-02-152</b></p><p><b>Endoscopic Evaluation of Upper Gastrointestinal Bleeding: Five-Year Study at Cipto Mangunkusumo National Referral Hospital</b></p><p><b>Virly Muzellina</b><sup>1</sup>, Liovicinie Andarini<sup>2</sup> and Ari Fahrial Syam<sup>1</sup></p><p><sup>1</sup><i>Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;</i> <sup>2</sup><i>Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate the causes of hematemesis and melena over the past five years, with a focus on improving patient outcomes and guiding clinical decisions through endoscopic examination.</p><p><b><i>Methods:</i></b> The study analyzed records of patients retrospectively who underwent upper gastrointestinal endoscopy at the Gastrointestinal Endoscopy Center in Cipto Mangunkusumo Hospital, Jakarta, Indonesia between 2019 and 2023.</p><p><b><i>Results:</i></b> Between 2019 and 2023, 5,721 patients underwent upper gastrointestinal endoscopy, with 1,090 (19.1%) due to upper gastrointestinal bleeding. Among these, 593 were male (54.4%) and 497 were female (45.6%), with a mean age of 54.4 ± 15.12 years. The largest age group affected was 40-59 years, comprising 451 cases (41.4%). Of the 1,090 patients, 262 (24%) presented with hematemesis, 654 (60%) with melena, and 174 (16%) with both symptoms. Non-variceal bleeding was identified in 913 cases (83.8%), while variceal causes accounted for 177 cases (16.2%). Erosive gastritis was the most frequent cause of upper GI bleeding (408 cases, 37.4%), followed by peptic ulcers (270 cases, 24.8%). Among peptic ulcer cases, 136 (50.4%) were located in the stomach. Additionally, cancer was a cause in 104 cases (9.5%), with duodenal cancer being the most common (48 cases, 46.2%).</p><p><b><i>Conclusion:</i></b> This study identified erosive gastritis as the leading cause of upper gastrointestinal bleeding, followed by peptic ulcers and varices.</p><p><b>PP-02-153</b></p><p><b>Haematemesis and melena from esofageal varices in Banti’s Syndrome : Rare Case</b></p><p><b>Anton Purnomo</b> and Febry Rahmayani and Suyata Suyata</p><p><i>RSUP dr. Mohammad Hoesin Palembang, Palembang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Introduction:</i></b> Haematemesis and melena are bleeding from the GI tract proximal to the ligament of Treitz. Non-cirrhotic portal hypertension is portal hypertension without cirrhosis, occurring in 10% of portal hypertension patients. Ultrasound examination and liver biopsy showed normal results. This disease is not easy to diagnose because of its low prevalence and varied clinical manifestations, generally in the form of esophageal varices, splenomegaly, and anemia. Banti syndrome is characterized by splenomegaly, pancytopenia due to hypersplenism, and portal hypertension, in the absence of liver abnormalities.</p><p><b><i>Case Presentation:</i></b> A 24 year old man was diagnosed, coming with complaints of vomiting blood and black stools since 8 hours before entering the hospital. The patient had no history of hepatitis, diabetes mellitus, and hypertension. The patient also never smoked, drank alcohol or used illegal drugs. Physical examination revealed pale palpebral conjunctiva and hepatosplenomegaly. From laboratory data, the Hb level was 9.3 g/dL, leukocytes 3,800/mm3, and platelets 70x103/mm3. A CT scan of the abdomen showed hepatomegaly, dilated portal veins and diffuse permagna splenomegaly with dilated splenic veins. spleen stiffness was found to be 60 kPa. After an endoscopic procedure, a picture of grade III esophageal varices and portal hypertensive gastropathy was obtained. Bleeding was stopped by ligation of the varices and administration of intravenous terlipressin.</p><p><b><i>Conclusion:</i></b> Banti’s syndrome is characterized by splenomegaly, pancytopenia due to hypersplenism, and portal hypertension in the absence of liver disease. Banti syndrome is a diagnosis of exclusion when all causes of portal hypertension, splenomegaly, and pancytopenia are excluded.</p><p><b>PP-02-154</b></p><p><b>Case series for the use of lanreotide in haemorrhagic hereditary telangiectasia (HHT) gastrointestinal bleeding</b></p><p>Farah Jaffar and <b>Chehkuan Tai</b></p><p><i>Barnet General Hospital, Royal Free NHS Foundation Trust, London, United Kingdom</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Haemorrhagic hereditary telangiectasia (HHT) can lead to severe anaemia due to gastrointestinal (GI) angiodysplasia and associated chronic bleeding. This can result in repeated hospital admissions for transfusions and endoscopic evaluation which can have a significant impact on morbidity and mortality.</p><p><b><i>Case description:</i></b> A 43 year old female with HHT complicated by GI angioectasia, pulmonary and hepatic arteriovenous malformations, had been significantly impacted by GI bleeding for over 10 years with the first angioectasia identified in 2013. Since then she has had annual endoscopies and was transfusion dependent due to severe anaemia. Most recently in June 2022 she had 173 angioectasias ablated with argon plasma coagulation (APC). She was subsequently started on a somatostatin analogue, lanreotide, in 2023 and has been free of hospitalisations, transfusions and endoscopic management since. A 62 year old female also with a background of HHT and duodenal and stomach angioectasia had recurrent hospital admissions with UGI bleeding resulting in endoscopic therapy with APC as well as clips. Her case is complicated by atrial fibrillation for which she cannot be anti-coagulated due to the frequent GI bleeding; this unfortunately resulted in a cerebrovascular accident leaving her with significant disabilities. Once initiated on lanreotide in February 2024, she has had no hospitalisations with GI bleeding.</p><p><b><i>Discussion:</i></b> These cases highlight the significant burden that HHT GI bleeding has on patients due to the multiple admissions, transfusions and endoscopies impacting their quality of life. The introduction of somatostatin analogues is seen to be an effective and safe pharmacological option.</p><p><b>PP-02-155</b></p><p><b>Efficacy of transcatheter arterial embolization for nonvariceal upper gastrointestinal hemorrhage</b></p><p><b>Pham Phuong Thu Tran</b> and Ngoc Luu Phuong Tran</p><p><i>Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Viet Nam</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Upper gastrointestinal bleeding (UGIB) is common emergency illness. Approximately 5% of patients experiencing recurrent bleeding after endoscopic therapies, facing the high death threats due to the limitations of current endoscopic equipment and techniques and patients with unstable comorbidity. The previous evidence illustrated that transcatheter arterial embolization (TAE) stood out as a safer and more effective alternative to surgery for these cases. This study aim to assess the efficacy of TAE within 7 days post-procedure in Vietnam.</p><p><b><i>Materials and Methods:</i></b> Cases study of patients ≥ 18-year-old who had nonvariceal UGIB with TAE treatment from January 1st, 2019, to September 30th, 2022, at Gia Dinh and Trung Vuong hospital.</p><p><b><i>Results:</i></b> We collected 64 nonvariceal UGIB cases treated by TAE. The most common bleeding site was the duodenal bulb (54.59% of cases). Ulcers larger than 1 cm were found in 59.38% of cases. Clearly bleeding evidence experienced in 78.12% of cases on endoscopy or abdominal CT. The technical and clinical success rate of TAE procedure were 96.87% and 73.44%, respectively. Serious complications accounted for 3.13%, including severe hypotension and liver ischemic. The in-hospital mortality rate was significant higher in patients with recurrent UIGB after TAE (OR 4.32, p = 0.02).</p><p><b><i>Conclusions:</i></b> Refractory nonvariceal UIGB occurs in patients with complex etiology of bleeding and serious medical history. Give its efficacy and safety, TAE should be considered to manage bleeding after unsuccessful endoscopic therapy. For future work, the proposed method should be compared with surgery to determine the optimal approach for these patients.</p><p><b>PP-02-157</b></p><p><b>Safety and efficacy of linaclotide in treating constipation in pediatric patients: a systematic meta-analysis</b></p><p>Ahmed Farag<sup>1</sup>, Mohamed Karam Allah Elkholy<sup>2</sup>, Wisam Al-Khaboori<sup>3</sup>, Amro Mamdouh Abdelrehim<sup>1</sup> and <b>Safia Elshennawy</b><sup>4</sup></p><p><sup>1</sup><i>College Of Medicine, Misr University For Science And Technology, 6th October city, Egypt;</i> <sup>2</sup><i>College of Pharmacy, Zagazig University, Zagazig, Egypt;</i> <sup>3</sup><i>College of Medicine, Mutah University, Al-Karak, Jordan;</i> <sup>4</sup><i>Shebin Elkom Teaching Hospital, Egypt</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Constipation is common in children and can be challenging to manage, especially in cases like functional constipation. Linaclotide, originally approved for adults, is now the only FDA-approved treatment for children with functional constipation. We aim to assess its safety and efficacy in this systematic meta-analysis.</p><p><b><i>Materials and Methods:</i></b> Our literature search covered MEDLINE via PubMed, Scopus, WOS, and Cochrane. We only included RCTs. Primary outcomes spontaneous bowel movements (SBM) and treatment-emergent adverse events (TEAEs).</p><p><b><i>Results:</i></b> Four studies with 639 patients were included. The overall effect estimates regarding SBM showed statistically significant results favoring linaclotide group [MD 1.09, 95 % CI (0.55–1.63), P = 0.0001] with a low heterogeneity (P = 0.29; I² = 19 %). Regarding Straining severity and stool consistency, the overall effect estimates favored linaclotide group [MD -0.39, 95 % CI (-0.57–-0.20), P = 0.0001], [MD 0.79, 95 % CI (0.32–1.26), P = 0.0009] respectively.</p><p>Regarding linaclotide safety in pediatric population, no statistically significant difference between groups were observed regarding any TEAEs [RR 1.13, 95 % CI (0.71–1.81), P = 0.61], any treatment-related TEAEs [RR 2.16, 95 % CI (0.89–5.22), P = 0.09], any serious adverse events [RR 0.69, 95 % CI (0.13–3.63), P = 0.66], or any adverse events leading to discontinuation [RR 0.75, 95 % CI (0.19–3.02), P = 0.69].</p><p><b><i>Conclusion:</i></b> This is the first meta-analysis on linaclotide for pediatric constipation. Our results suggest that linaclotide demonstrates promising efficacy and is generally well-tolerated. Current evidence is insufficient; further high-quality, large-scale randomized controlled trials are required.</p><p><b>PP-02-158</b></p><p><b>Revisiting the Starting Age of Colorectal Cancer Screening for Average-Risk Asian Population: a cost-effectiveness analysis</b></p><p><b>Junjie Huang</b><sup>1</sup>, Victor Chan<sup>1</sup>, Mingtao Chen<sup>1</sup>, Jamie Jie Mei Liew<sup>1</sup>, Xiangjing Liu<sup>2</sup>, Chaoying Zhong<sup>3</sup>, Jianli Lin<sup>4</sup>, Junjie Hang<sup>5</sup>, Claire Chenwen Zhong<sup>1</sup>, Jinqiu Yuan<sup>6</sup>, Wanghong Xu<sup>7</sup>, Mellissa Withers<sup>8</sup>, Andrew Chan<sup>9</sup> and Martin Wong<sup>1</sup></p><p><sup>1</sup><i>The Chinese University Of Hong Kong, Sha Tin, Hong Kong;</i> <sup>2</sup><i>Erasmus MC University Medical Center, The Netherlands;</i> <sup>3</sup><i>Guangdong Ocean University, China;</i> <sup>4</sup><i>Peking University, China;</i> <sup>5</sup><i>Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China;</i> <sup>6</sup><i>Sun Yat-Sen University, China;</i> <sup>7</sup><i>Fudan University, China;</i> <sup>8</sup><i>University of Southern California, United States;</i> <sup>9</sup><i>Massachusetts General Hospital, United States</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and aims:</i></b> This study aims to assess the cost-effectiveness of different starting ages for colorectal cancer (CRC) screening among an Asian population.</p><p><b><i>Methods:</i></b> We conducted a simulation study involving 100,000 individuals in Hong Kong who were screened using either fecal immunochemical test (FIT) or colonoscopy as primary screening methods at ages 40, 45, and 50 until age 75. Individuals with a positive FIT result subsequently underwent colonoscopy. The performance of different strategies was evaluated based on life-years gained, and cost-effectiveness was measured using the incremental cost-effectiveness ratio (ICER).</p><p><b><i>Results:</i></b> The ICERs for initiating FIT screening at ages 40, 45, and 50 were USD 134,887, USD 108,178, and USD 87,362, respectively. For colonoscopy, the ICERs for initiating screening at ages 40, 45, and 50 were USD 592,767, USD 501,748, and USD 432,029, respectively. Overall, the FIT strategy was found to be less costly. The highest number of life-years gained was observed when colonoscopy screening was initiated at age 40 (2,070), followed by age 45 (1,923) and age 50 (1,714). In terms of FIT, the most life-years gained were observed at age 40 (1,652), followed by age 45 (1,515) and age 50 (1,323).</p><p><b><i>Conclusion:</i></b> Initiating CRC screening at age 45 using FIT in Hong Kong was determined to be a well-balanced and cost-effective strategy. This approach demonstrated a cost advantage over starting screening at age 40 and resulted in more lives saved compared to screening at age 50.</p><p><b>PP-02-159</b></p><p><b>Incidence of venous thromboembolism in Asian patients with inflammatory bowel disease: systematic review and meta-analysis</b></p><p><b>Kyu Chan Huh</b><sup>1</sup>, Dae Sung Kim<sup>1</sup>, Hoon Sup Koo<sup>1</sup>, Sung Ryul Shim<sup>2</sup> and Joo Hye Song<sup>3</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Konyang University College of Medicine, Daejeon, South Korea;</i> <sup>2</sup><i>Department of Biomedical Informatics, College of Medicine, Konyang University, Daejeon, South Korea;</i> <sup>3</sup><i>Department of Internal Medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and aims:</i></b> Although the incidence and prevalence of inflammatory bowel disease (IBD) have been gradually increasing throughout Asia, the incidence of venous thromboembolism (VTE) in Asia is relatively lower than that in Western countries. This study aimed to evaluate the incidence of VTE in Asian IBD patients using a systematic review and meta-analysis.</p><p><b><i>Methods:</i></b> Studies were identified through a literature search of the PubMed, Embase, and Cochrane databases (from inception inclusive April 2024) for English studies. The criteria for selecting the participants were as follows: (1) studies including patients with Crohn's disease (CD) and Ulcerative colitis (UC) in the Asian population; (2) comparisons were specified as with a control group of non-IBD patients for comparative incidence; and (3) outcomes were measured by relative risks (RR) and hazard risk (HR) for VTE incidence in nationwide cohort studies.</p><p><b><i>Results:</i></b> Five studies met the inclusion criteria. The pooled RR for overall VTE incidence in Asian patients with IBD compared to that in non-IBD patients was 2.065 (95% CI: 1.905–2.238). The heterogeneity test resulted in a p-value of 0.418 for the Cochrane Q statistics, and Higgins’ I2 was 0%. There was no statistical moderating effect of the variables (number of patients, mean age, female rate, CD proportion, country, and type of risk) on the outcomes.</p><p><b><i>Conclusions:</i></b> The VTE incidence in Asian patients with IBD was higher than that in non-IBD patients (RR 2.065). It seemed reasonable to consider prophylaxis for VTE in hospitalized patients with IBD.</p><p><b>PP-02-160</b></p><p><b>CHIA deficiency in the chief cells promotes SPEM through pyroptosis mediated M2 Macrophage polarization</b></p><p><b>Shuhui Liu</b><sup>1</sup>, Yingying Zhao<sup>1</sup>, Zhiyuan Ma<sup>1</sup>, Chunli Hu<sup>1</sup>, Jiaxing Zhu<sup>1</sup>, Biguang Tuo<sup>1</sup>, Taolang Li<sup>2</sup> and Xuemei Liu<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China;</i> <sup>2</sup><i>Department of general surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Loss of parietal cells causes the development of spasmolytic polypeptide-expressing metaplasia (SPEM) through transdifferentiation of chief cells. However, whether initial chief cell loss cause SPEM needs to be further investigated. CHIA is highly expressed in the chief cells, and has an important role in gastric mucosal immune homeostasis. However, the molecular role and mechanism of CHIA in the development of SPEM is need further investigated.</p><p><b><i>Methods:</i></b> single-cell sequencing (scRNA-seq) technology, histopathological and immunofluorescence (IF).</p><p><b><i>Results:</i></b> PCR and scRNA-seq technology data showed that increased M2 macrophage infiltration and SPEM formation, but remaining of parietal cells in CHIA KO mice, when compared to CHIA WT mice. Furthermore, deletion of CHIA in mice resulted in release of endoplasmic reticulum stress-triggered, including upregulation of endoplasmic reticulum stress markers, followed with upregulation of pyroptosis-related marker, as well as significantly increasement of IL33/IL-13 release. Moreover, Double immunoflorescence staining showed that upregulation and colocalization of M2 macrophage polarization markers, including F4/80 and CD163+ in the gastric mucosal of CHIA KO mice. Notably, IF showed that CHIA deficiency caused the mucous neck cell marker SOX9 shifted from the cervical isthmus into the SPEM cells, and co-localized with the SPEM markers CD44v9, TFF2 and MUC6, suggesting that the origin of SPEM cells may transdifferentiate from mucous neck cell after CHIA gene deletion.</p><p><b><i>Conclusions:</i></b> CHIA deficiency in the chief cells promotes SPEM through the pyroptosis mediated M2 macrophage polarization. The origin of CHIA deficiency promotes injury may origin from the transdifferentiation of mucous neck mucus cells into SPEM cells.</p><p><b>PP-02-162</b></p><p><b>The nutritional analysis of South Korean infants from birth to 2 years</b></p><p><b>Yeji Ksim</b><sup>1</sup>, Youn Ha Kang<sup>1</sup>, Sun Woo Park<sup>1</sup>, Jeong Eun Ahn<sup>1</sup>, Lia Kim<sup>1</sup>, Homin Huh<sup>1</sup>, Jong Woo Hahn<sup>2</sup>, Minsoo Shin<sup>3</sup>, Jin Gyu Lim<sup>1</sup>, Ki Soo Kang<sup>4</sup>, Yeoun Joo Lee<sup>5</sup>, Jee Yoon Park<sup>8</sup>, Chan-Wook Park<sup>8</sup>, Joo Young Kim<sup>9</sup>, Su Jin Jeong<sup>10</sup>, Young Hwa Jung<sup>2</sup>, Jae Hyun Kim<sup>2</sup>, Chang Won Choi<sup>2</sup>, Ju Whi Kim<sup>1</sup>, Seung Han Shin<sup>1</sup>, Yun Jeong Lee<sup>1</sup>, Young Ah Lee<sup>1</sup>, Choong-Ho Shin<sup>1</sup>, Seung-sik Hwang<sup>11</sup>, M.S Young Eun Kim<sup>1</sup>, Jae Sung Ko<sup>1</sup> and Jin Soo Moon<sup>1</sup></p><p><sup>1</sup><i>Department of Pediatrics, Seoul National University College of Medicine, Seoul, South Korea;</i> <sup>2</sup><i>Department of Pediatrics, Seoul National University Bundang Hospital, Bundang, South Korea;</i> <sup>3</sup><i>Department of Pediatrics, Korea National University College of Medicine, Seoul, South Korea;</i> <sup>4</sup><i>Department of Pediatrics, Jeju National University College of Medicine, Jeju, South Korea;</i> <sup>5</sup><i>Department of Pediatrics, Pusan National University School of Medicine, Pusan, South Korea;</i> <sup>6</sup><i>Department of Pediatrics, Soonchunhyang University College of Medicine, Seoul, South Korea;</i> <sup>7</sup><i>Department of Pediatrics, Korea University Guro Hospital, Seoul, South Korea;</i> <sup>8</sup><i>Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea;</i> <sup>9</sup><i>Department of Pediatrics, Eulji University College of Medicine, Seoul, South Korea;</i> <sup>10</sup><i>Department of Pediatrics, CHA Bundang Medical Center, School of Medicine, CHA University, Bundang, South Korea;</i> <sup>11</sup><i>Department of Public Health Science, Graduate School of Public Health, Seoul National University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> From a prospective national South Korean children's growth cohort, the nutritional analysis of healthy South Korean infants whose growth parameters lay between the two standard deviation z score for height-for-age, weight-for-age, BMI-for-age were analyzed.</p><p><b><i>Materials and Methods:</i></b> A longitudinal study of Korea infant physical growth experimental survey (KIPGros) (n=256) was taken and children aged <24 mo (n= 72) was evaluated. The mean value of growth parameters of each group: younger infants (4 mo – 5.9mo), older infants (6-11.9 mo), toddlers (12-23.9) were calculated. Only the growth parameter which laid within the two standard deviations of the WHO z score of height, weight, and BMI were selected. The Nutritional intake was analyzed and calculated for calorie, protein, fat and carbohydrate by a clinical dietitian, using a Computer Aided Nutritional Analysis program.</p><p><b><i>Results:</i></b> Out of the total enrolled number n=256, a smaller subset of 72 children were analyzed. Height-weight-BMI values that lay beyond the two standard deviations were eliminated. The mean height-weight-BMI of younger infants (4mo – 5.9mo) were 64.8cm-7.5kg-17.8m2/kg, older infants (6mo -11.9mo) 70.8cm-8.8kg-17.6m2/kg, toddlers (12mo - 23.9mo) 80.3cm-10.6kg-16.2m2/kg. The mean calorie, protein, fat, and carbohydrate were as follows: younger infants (4mo – 5.9mo) 511.5kcal, 13.0g, 23.4g, 61.3g, older infants (6mo -11.9mo) 532.5kcal, 18.4g, 18.8g, 71.4g, toddlers (12mo -23.9mo) 992.7kcal, 41.7g, 30.7g and 136g.</p><p><b><i>Conclusion:</i></b> This was a prospective cohort study of the growth and nutritional analysis of Healthy Korean infants from 0 to 24 months. We hope our outcome will serve as a future growth reference and dietary guidance.</p><p><b>PP-02-163</b></p><p><b>Burden of cancer incidence/mortality in the China, 1980-2021: a analysis for the GBD 2021</b></p><p><b>Rong Lin</b></p><p><i>Huazhong University of Science and Technology, Wuhan, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> China has gone through changes in its social and economic sectors. Thus, efforts need to include thorough evaluations of cancer burden in China.</p><p><b><i>Materials and Methods:</i></b> Using data from GBD 2021, we first provide estimates of the incidence, mortality and the contribution of risk factors from 1990/1980 to 2021 in China.</p><p><b><i>Results:</i></b> In 2021, neoplasms accounted for 24.07% (95% uncertainty interval 22.74 to 25.29) of total deaths and 17.7% (15.21 to 20.2) of total DALYs in both sexes in the China. In the same period, age-standardized incidence rate (ASIR) and age-standardized deaths rate (ASDR) was 790.17 (676.83 to 926.32) and 137.48 (115.11 to 163.38), respectively. In both sexes in 2021, tracheal, bronchus, and lung cancer showed the highest ASIR (44.01), followed by non-melanoma skin cancer (37.54), colon and rectum cancer (31.44), stomach cancer (29.05), breast cancer (19.36), esophageal cancer (15.04), and liver cancer (9.52). In a word, the ASDR of neoplasms decreased about 29.78% in males and 42% in females in the past forty years in China. Lung cancer showed the highest ASIR (62.63) and ASDR (56.45) in males. Of note, 31.73% of all cancer cases death in China were digestive cancers in 2021. In level 1 risks in 2021, behavioral risks was linked to 73.57% of neoplasm in the all age group.</p><p><b>PP-02-164</b></p><p><b>Burden of digestive disease among adolescents in China, 1990-2021: a analysis of the GBD 2021</b></p><p><b>Rong Lin</b></p><p><i>Huazhong University of Science and Technology, Wuhan, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There is limited data on the prevalence and risk factors of digestive diseases among adolescents and young adults in the country.</p><p><b><i>Materials and Methods:</i></b> Using data from GBD 2021, we first provide estimates of the prevalence and DALYs burden of digestive disease from 1990 to 2021 in the childhood and adolescence.</p><p><b><i>Results:</i></b> In 2021, the reported mean prevalence of digestive disease in the age 10 to 24 years group in China was 11.05% (95% UI: 9.81%-12.41%) and accounting for 233,736 (95% UI: 182,862-294,871) DALYs of a total of 10,374,141 DALYs for both sexes. Upper digestive system diseases contributed to approximately 1/3rd (27.4%) of total DALYs followed by gallbladder and biliary diseases (23.3%), cirrhosis and other chronic liver diseases (18.2%), appendicitis (7.5%), inguinal, femoral, and abdominal hernia (7.2%), paralytic ileus and intestinal obstruction (6.5%), other digestive diseases (4.1%), pancreatitis (4%), IBD (1.7%), and vascular intestinal disorders (0.2%). There was some variation in age-related sex-specific patterns. Males had slightly higher overall prevalence and DALYs attributed to digestive disease as compared to females in the 10-24 age group, especially in 20-24 age group. While females had slightly higher overall prevalence (1.41%, 95% UI:1.07%-1.86%) and DALYs 33,609 (95% UI:21,185-50,616) attributed to gallbladder and biliary diseases than male prevalence (0.78%, 95% UI:0.59%-1.04%) and DALYs 20,868 (95% UI:13,132-31,728).</p><p><b><i>Conclusion:</i></b> The prevalence and DALYs has been decreasing in children and adolescents of digestive disease in China. Differentiated intervention and outreach strategies based on age and gender would be necessary to reduce the impact of digestive disease.</p><p><b>PP-02-165</b></p><p><b>The Hidden Culprit: Lead Poisoning in Anemia and Abdominal Pain</b></p><p><b>Fei Yang Pan</b><sup>1,2</sup> and Brandon Baraty<sup>1,2</sup></p><p><sup>1</sup><i>Macquarie University Hospital, Sydney, Australia;</i> <sup>2</sup><i>Macquarie University, Sydney, Australia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Lead poisoning is rare in developed countries but can occur with traditional medicines or environmental exposures. Acute lead exposure often presents with neurological symptoms and abdominal pain. Hemolytic anemia is a rare complication.</p><p><b><i>Case Description:</i></b> A 42-year-old female with no significant medical history presented to the ED on June 4, 2024, with abdominal pain, nausea, chest tightness, and acute difficulty breathing. The pain was severe, fluctuating, and troubling at night. She had been taking traditional Siddha medicines in South India from January to April 2024, stopping just before admission in June.</p><p>Her hemoglobin level was 84 g/L, with normal bilirubin, lactate dehydrogenase (LDH) levels, and haptoglobin. Extensive investigations for severe abdominal pain and anemia, including a CT scan, gastroscopy, and colonoscopy, were normal. She required three RBC transfusions. Further investigation with a video capsule endoscopy showed terminal ileum erosions, and a labelled red cell scan indicated a likely bleed in the descending colon. Repeat gastroscopy and colonoscopy were normal.</p><p>Multiple blood films did not show basophilic stippling, but a repeat film on July 9 did. Haptoglobin was positive for hemolysis on July 3, with elevated bilirubin and LDH levels. A heavy metal screen revealed elevated lead levels (44.8 μg/dL). Her herbal medications contained lead levels of 13,522.35 μg/g.</p><p><b><i>Discussion:</i></b> This case shows that basophilic stippling and a positive hemolytic screen may not always be present in acute lead poisoning with anemia. Clinical suspicion must be high, especially in patients using traditional medicines, which can be a significant lead source.</p><p><b>PP-02-166</b></p><p><b>Recurrent Gastrointestinal Bleeding Caused by Small Bowel Hemangiolymphangioma: A Rare Case</b></p><p><b>Mita Hafsah Saraswati</b><sup>1</sup> and Virly Muzellina<sup>2</sup></p><p><sup>1</sup><i>Universitas Indonesia Hospital/ Dr. Cipto Mangunkusumo National Central General Hospital, Depok/ Jakarta, Indonesia;</i> <sup>2</sup><i>Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hemangiolymphangioma or Hemolymphangioma is rare malformation composed of both lymphatic and vascular vessels. Small bowel hemangiolymphangiomas are extremely rare, with less than 1% of all lymphangiomas. To our knowledge, only 10 small bowel hemangiolymphangioma cases have been reported. Here, we report a hemangiolymphangioma case, which present with gastrointestinal bleeding and anemia.</p><p><b><i>Case Description:</i></b> A 34-year-old female complained of recurrent episodes of melena and easily fatigue for more than 2 months. On physical examination, anemic conjunctiva and upper abdominal tenderness were noted. Laboratory analysis revealed severe anemia with hemoglobin of 6.3 g/dL and fecal occult blood was positive. Esophagogastroduodenoscopy showed antral gastritis and colonoscopy result was normal. An anterograde balloon- assisted enteroscopy was performed, and a protruded mass was revealed in the proximal jejunum. Histology from endoscopic biopsies confirmed the diagnosis of hemangiolymphangioma. Later, the patient underwent partial resection of the small intestine. At the 6 months follow-up, no recurrence of melena or anemia was observed.</p><p><b><i>Discussion:</i></b> Hemangiolymphangioma is an uncommon vascular malformation characterized by the proliferation or network formation of vascular spaces, which can include lymphatics, capillaries, veins, or arteries. It is rare among adults. Hemangiolymphangioma often difficult to diagnose due to their location and the limited visibility of conventional endoscopy. However, advances in diagnostic tools such as balloon-assisted enteroscopy have improved the detection of these malformations. This case highlights the importance of considering hemangiolymphangioma as a cause of recurrent gastrointestinal bleeding.</p><p><b>PP-02-167</b></p><p><b>Gastrointestinal (GI) Bleeding With Anti-thrombotic Use: A Large Malaysian Cohort Study</b></p><p><b>Shahreedhan Shahrani</b><sup>1</sup>, Sandeep Singh Gill<sup>2</sup>, Xin Tong Ng<sup>2</sup>, Kah Hee Looi<sup>2</sup>, Nurul Safirah Norhisyam<sup>2</sup>, Sher Weyne Chee<sup>2</sup>, Xin Hui Khoo<sup>1</sup> and Sanjiv Mahadeva<sup>2</sup></p><p><sup>1</sup><i>Gastroenterology Unit, Department of Internal Medicine, University Malaya Medical Centre, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There have been limited reports studying GI bleeding due to anti-thrombotic use in a diverse, large population, especially in South East Asia. This study aims to assess the relation of anti-thrombotics with GI bleeding; to describe its demographics, clinical characteristics and endoscopic outcomes.</p><p><b><i>Materials and Methodology:</i></b> This study was a retrospective audit in University Malaya Medical Centre (UMMC). All patients referred for suspected GI bleeding and underwent GI endoscopy (gastroscopy and colonoscopy) from 1/1/2013 to 31/12/2021 were included. Patients were identified from manual endoscopy logbooks. Demographic and relevant information including endoscopic outcomes were charted via the hospital’s electronic medical records (EMR) database.</p><p><b><i>Results:</i></b> There were 5222 patients referred for GI endoscopy due to suspected GI bleeding. Peptic ulcer was the main cause in the upper tract (28.3%) and hemorrhoids, in the lower tract (17.4%). 1548 patients (29.6%) were on anti-thrombotics; 337 patients (6.5%) were on anticoagulants and 1211 patients (23.2%) were on antiplatelets. The most common anticoagulant use that presented with GI bleeding were warfarin (33%), apixaban (28.9%) and dabigatran (18%). Antiplatelets that presented with GI bleeding were mainly aspirin (12.9%) and clopidogrel (3.8%). Use of warfarin, apixaban and rivaroxaban were significant risks for GI bleeding (p<0.001).Age, presence of comorbidities such as hypertension, chronic kidney disease, ischaemic heart disease and atrial fibrillation; and aspirin use were also found to be significant risk factors for GI bleeding. (p<0.001)</p><p><b><i>Conclusion:</i></b> Significant risk factors for GI bleeding were age, comorbidities , antiplatelet use and usage of warfarin, apixaban and rivaroxaban.</p><p><b>PP-02-168</b></p><p><b>Small intestinal arteriovenous malformation presenting as recurrent obscure, overt gastrointestinal bleeding in a 34-year-old male</b></p><p><b>Jonathan Viernes</b>, Juvaline Valete and Caesar Noel Palaganas</p><p><i>Baguio General Hospital and Medical Center, Baguio City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Small intestinal bleeding poses a diagnostic challenge. Vascular lesions are common lesions of the small intestine. However, arteriovenous malformations in the small intestines are rare. They are commonly located in the cecum and right side of the colon.</p><p><b><i>Case report:</i></b> Patient V. G., a 34-year-old male, Filipino, was admitted due to recurrent episodes of hematochezia which started about 9 months prior to admission. Upper and Lower GI endoscopy done at another local hospital 4 months prior to admission only revealed non-specific colitis and erosive gastritis. Repeat endoscopy was done at our institution and revealed Non-erosive Gastritis and blood clots were seen from the cecum to the rectum. Due to persistent hematochezia, mesenteric angiogram was done revealing multiple tortuous dilated vessels tangled in the left hemiabdomen draining some segments of the jejunum. On exploratory laparotomy, arteriovenous malformations were seen in the jejunum, 30 centimeters from the ligament of Treitz, with involved segment of 50 centimeters. Intraoperative enteroscopy revealed multiple dilated vessels with oozing blood. Segmental resection with primary anastomosis was done. Histopathologic examination of the resected segment was consistent with arteriovenous malformation. The patient’s condition improved.</p><p><b><i>Discussion:</i></b> Arteriovenous malformation in the small intestines is a rare cause of GI bleeding. Preoperative diagnosis and localization of arteriovenous malformations of the small intestines is important prior to any intervention. In this case report, mesenteric angiogram and intraoperative enteroscopy played a useful role in the diagnosis. Treatment for AV malformations of the small intestines is surgical resection.</p><p><b>PP-02-169</b></p><p><b>A Systematic Review of Adverse Effects Associated with Proton Pump Inhibitor Long-Term Use</b></p><p><b>Hayati Alwani Yahaya</b></p><p><i>National University Of Malaysia, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Proton pump inhibitors (PPIs) are commonly prescribed for the treatment of acid-related disorders. Prolonged use of PPIs may be associated with several potential adverse effects, raising questions about the risk-benefit balance of extended therapy. This systematic review seeks to address these concerns by evaluating published literature that investigates the long-term adverse effects of PPIs use, aiming to provide a comprehensive understanding of its safety profile.</p><p><b><i>Methods:</i></b> We conducted a comprehensive literature search across PubMed, Scopus and Web of Science for observational studies published up to 2024. Our search strategies included controlled vocabulary and related keywords: adverse effects of long-term PPIs use. Two authors independently screened the articles for inclusion, with a third author resolving disagreements. Quality and risk of bias were evaluated using the Newcastle-Ottawa Scale.</p><p><b><i>Results:</i></b> A total of 45 studies were included in the review. Prolonged PPIs use was associated with a range of long-term effects. The most common were increased bone fragility, followed by higher susceptibility to enteric infections, possible risk of gastric cancer and impaired micronutrient absorption. The duration associated to adverse effects was 4 weeks with some studies indicating higher risks associated with extended use. Most adverse effects were reported in observational studies with consistent results and significant confounding risks.</p><p><b><i>Conclusion:</i></b> Long-term PPIs use is associated to several potential adverse effects, though evidence strength varies. It is crucial to weigh the risks and benefits of extended PPI therapy. A multidisciplinary approach from healthcare providers is needed to provide better recommendations for PPIs prescribing.</p><p><b>PP-02-170</b></p><p><b>Metabolic syndrome and the risk of colorectal cancer in individuals younger than 50 years</b></p><p><b>Jung Eun Yoo</b><sup>1,2</sup> and Eun Hyo Jin<sup>3,4</sup></p><p><sup>1</sup><i>Department of Family Medicine, Seoul National University Hospital Healthcare System Gangnam Center, Seoul, South Korea;</i> <sup>2</sup><i>Department of Family Medicine, Seoul National University College of Medicine, Seoul, South Korea;</i> <sup>3</sup><i>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea;</i> <sup>4</sup><i>Department of Internal Medicine, Healthcare Research Institute, Seoul National University Hospital Healthcare System Gangnam Center, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Metabolic Syndrome (MetS) is a cluster of diverse conditions that has been linked to an increased risk of early onset colorectal cancer (CRC). However, the impact of evolving MetS status over time on early onset CRC risk remains unknown.</p><p><b><i>Materials and Methods:</i></b> Eligible participants from the National Health Insurance Service database in South Korea were followed up till 31 December 2011. In total, 3,340,635 individuals who underwent health examinations between 2009 and 2011 were categorized into four groups based on changes in MetS status: MetS-free (no-no), recovered (yes-no), developed (no-yes), and persistent (yes-yes). CRC risk was assessed for the 20–39 and 40–49 years groups until the end of 2019.</p><p><b><i>Results:</i></b> Among the 20–39-year-old’s, those with persistent MetS had a 16% higher risk of CRC, whereas those in the 40–49 years old group had a 22% higher risk, even after adjusting for covariates. The subgroup analysis revealed differences in risk based on sex, alcohol consumption, and diabetes. Persistent MetS was associated with a 34% higher risk of distal colon cancer in the 40–49 years old group. Similarly, in the 20–39 year old’s, it was linked to a 29% higher risk of rectal cancer.</p><p><b><i>Conclusion:</i></b> Persistent MetS significantly increases the risk of early onset CRC in both age groups (20–39 and 40–49 years). This underscores the importance of actively managing MetS, particularly in the 20–39 years age group, to mitigate future CRC development. Therefore, tailored screening strategies should be considered for individuals with persistent MetS, even for those aged <50 years.</p><p><b>PP-02-171</b></p><p><b>Accelerometer-Derived “Weekend Warrior” Physical Activity and Digestive Diseases: a nationwide cohort study</b></p><p><b>Shiyi Yu</b><sup>1,2,3,4</sup>, Jing Feng<sup>1,2</sup>, Chun Ling Chen<sup>1,2</sup>, Qinming Li<sup>1,2</sup>, Weihong Sha<sup>1,2</sup> and Hao Chen<sup>1,2</sup></p><p><sup>1</sup><i>Department Of Gastroenterology, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, China;</i> <sup>2</sup><i>The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China;</i> <sup>3</sup><i>HKUMed Laboratory of Cellular Therapeutics, the University of Hong Kong, Hong Kong, SAR, China;</i> <sup>4</sup><i>Faculty of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Objective:</i></b> To investigate associations between accelerometer-derived weekend warrior patterns and more evenly spread MVPA with the risk of incident digestive diseases.</p><p><b><i>Design:</i></b> Population-based cohort study.</p><p><b><i>Participants:</i></b> The study included 89,595 UK Biobank participants with wrist-based accelerometer data.</p><p><b><i>Exposures:</i></b> Participants were categorized into three physical activity patterns: active weekend warriors (active WW, achieving 150+ minutes with ≥50% of MVPA on 1-2 days), active regulars (meeting 150+ minutes but not active WW), and inactive (less than 150 minutes). These patterns were also assessed using a sample median threshold of 230.4+ minutes of MVPA per week.</p><p><b><i>Main outcome measures:</i></b> Incidence of 16 digestive diseases.</p><p><b><i>Results:</i></b> Over a median follow-up of 7.9 years, both active patterns were associated with lower risks of incident digestive diseases compared to the inactive group, at both the guideline-based (≥150 minutes) and median (≥230.4 minutes) thresholds. At the guideline-based threshold (≥150 minutes), multivariable-adjusted hazard ratios (HRs) for weekend warriors and regularly active participants were 0.83 (0.79-0.87) and 0.79 (0.75-0.84) for any digestive disease; 0.67 (0.57-0.80) and 0.63 (0.51-0.78) for NAFLD; 0.89 (0.84-0.95) and 0.84 (0.78-0.90) for diverticulosis; 0.82 (0.76-0.90) and 0.85 (0.77-0.94) for constipation; 0.74 (0.66-0.82) and 0.70 (0.61-0.80) for cholelithiasis; 0.79 (0.74-0.85) and 0.76 (0.70-0.82) for GERD.</p><p><b><i>Conclusion:</i></b> Both weekend warriors and regular physical activity participants exhibited lower risks of incident digestive diseases compared to inactive individuals. Physical activity concentrated within 1-2 days was associated with a similarly lower risk of any digestive disease, NAFLD, diverticulosis, constipation, cholelithiasis, and GERD as more evenly distributed activity.</p><p><b>PP-02-172</b></p><p><b>Relationship of Neutrophil-to-Lymphocyte Ratio and morbidity among patients with cirrhosis admitted in a tertiary hospital</b></p><p><b>Ameer Hussein Abbas</b> and Johaniya Dimaporo and Abdel Hussein Lucman</p><p><i>Department Of Health, Amai Pakpak Medical Center, Marawi, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This is a baseline study in a tertiary government hospital in Lanao Del Sur, Philippines among patients admitted with cirrhosis. This also studies the relationship between Neutrophil-to-Lymphocyte Ratio (NLR) and morbidity in terms of the length of hospital stay, and in-hospital mortality. NLR is an inexpensive, objective, and widely available biomarker.</p><p><b><i>Materials and Methods:</i></b> This study analyzed all available records of patients admitted with cirrhosis from January-2018 to December-2022. Demographic characteristics, etiology, complications, length of stay, and mortality were documented. Prognostic markers namely NLR, CPC, MELD, and FIB4 were computed. NLR of each patient was analyzed in relation to patient outcome using Chi-square and Mann-Whitney test.</p><p><b><i>Results:</i></b> A total of 145 patient records were analyzed. Results showed that majority of the patients diagnosed with cirrhosis are men (59%); aged 60 and above (57%); with etiology of NAFLD (30%), schistosomiasis (24%), and hepatitis B (21%); patients sought consult during the onset of complications mostly due to ascites (33%), jaundice (19%), and abdominal pain (17%); and with an early score of CPC and MELD. Majority of the patients were discharged improved. In-hospital mortality rate was 5%. NLR of patients was categorized into ≤ 5, and greater than 5. No statistical significance was seen between NLR and the length of hospital stay (p value= 0.5), and in-hospital mortality (p value= 0.09).</p><p><b><i>Conclusion:</i></b> Most of the etiologies of patients admitted with cirrhosis are preventable. No statistical significance was seen between NLR and the length of hospital stay, and in-hospital mortality in this study.</p><p><b>PP-02-173</b></p><p><b>Serum microRNA signature as biomarkers for prediction of decompensation in patients with cirrhosis</b></p><p><b>Yingjie Ai</b>, Sitao Ye, Xiaoquan Huang and Shiyao Chen</p><p><i>Zhongshan Hospital, Shanghai, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Cirrhosis is a worldwide health concern and could attribute to different etiologies. Evolvement into decompensation often leads to poor outcomes. Therefore, noninvasive and accurate method for prediction and detection is in urgent need. This study aimed to identify microRNAs(miRNAs) as potential predictive biomarkers.</p><p><b><i>Materials and Methods:</i></b> MiRNA sequencing with serum sample from 16 cirrhosis patients was conducted. They were divided into two groups (8CE vs. 8nCE) according to occurrence of decompensated events including variceal bleeding, carcinoma, PVT, ascites and SBP. The expression pattern of candidate miRNAs was further tested in a validation cohort (20CE vs. 20nCE) with RT-PCR assays.</p><p><b><i>Results:</i></b> Six differentially expressed miRNAs were identified with 3 different methods among which five were downregulated while one was upregulated. Logistic regression showed that decreased miR195-5p and miR1287-5p were associated with cirrhosis progression (p = 0.03, 0.04). Their change pattern was further confirmed in the validation cohort. These two miRNAs shared 686 downstream target genes which may account for decompensation as they were involved in pathways like Hepatocellular carcinoma, EGFR tyrosine kinase inhibitor resistance, etc. Hub genes including EGFR, PTEN, BDNF, PPARs were also tested by RT-PCR and presented corresponding dysregulation.</p><p><b><i>Conclusion:</i></b> MiR195-5p and miR1287-5p could act as a potential biomarker in prediction of decompensation and help provide further personalized treatment to improve outcomes.</p><p><b>PP-02-174</b></p><p><b>Outcomes of a no prophylactic transfusion strategy in patients with cirrhosis undergoing high-risk invasive procedures</b></p><p><b>Sagnik Biswas</b>, Manas Vaishnav, Shekhar Swaroop, Arnav Aggarwal, Ayush Agarwal, Anshuman Elhence, Tushar Sehgal, Mukul Aggarwal, Rishi Dhawan, Deepak Gunjan, Shivanand Gamanagatti and Shalimar</p><p><i>All India Institute Of Medical Sciences, New Delhi, New Delhi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Limited data exists on the outcomes of performing high-risk procedures in patients with cirrhosis and platelet counts <50 x 10<sup>9</sup>/L, without any prophylactic platelet transfusion.</p><p><b><i>Methods:</i></b> Data from two prior randomized controlled trials (CTRI/2017/12/010822, and CTRI/2021/05/033464) on high-risk procedures in patients with cirrhosis were combined. We included patients with cirrhosis and severe thrombocytopenia while excluding patients with acute-on-chronic liver failure, renal dysfunction and patients on antiplatelet agents or anticoagulants. Patients were divided into two groups: (i) Standard-of-care: all patients received prophylactic platelet transfusions, and (ii) On-demand: no patients received any prophylactic transfusion. Outcome measures included major bleeding within 5-and 28-days of the procedure, requirement of blood transfusion and bleeding-related mortality.</p><p><b><i>Results:</i></b> Fifty patients received prophylactic transfusions while 68 patients did not. Baseline demographics were well matched.</p><p>Median Child and MELD score of the cohort was 6 (5-7) and 12 (10-14.3) respectively. Median platelet count was 35.5 (28.0-44.3) x 10<sup>9</sup>/L. Percutaneous liver biopsy was the most common procedure (73 patients, 61.9%). No major procedural bleeding or bleeding-related mortality occurred within 28 days of the procedure. One patient (2%) in the SOC arm and 4 (5.8%) in the on-demand arm had minor (WHO Grade 1) bleeding. Only 5 (7.4%) patients in the on-demand arm received platelet transfusions (17 units) as compared to all 50 patients (150 units) in the SOC arm.</p><p><b><i>Conclusion:</i></b> High-risk procedures can be performed safely in a select group of cirrhosis patients without prophylactic platelet transfusions. This would reduce empirical and irrational transfusion practices in these patients.</p><p><b>PP-02-175</b></p><p><b>The Correlation of Plasma Creatinin to Albumin Ratio for Prognosis and Fibrosis in Cirrhotic Patients</b></p><p><b>Indro Buono</b><sup>1</sup>, Rusdiyana Ekawati<sup>1</sup>, Titong Sugihartono<sup>2</sup> and Budi Widodo<sup>2</sup></p><p><sup>1</sup><i>Internal Medicine Subspeciality (Sp-II) Study Program, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga- Dr. Soetomo General Academic Hospital, Surabaya, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo General Academic Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Renal impairment is a common complication of cirrhosis, reported in up to one-third of hospitalized patients with advanced liver disease. Albumin is exclusively synthesized by hepatocytes, so that decrease in liver cirrhosis. Liver fibrosis is an important predictor of progression and prognosis in cirrhosis. Plasma Creatinin-Albumin ratio (CAR) can be proposed as a simple, affordable, and easily accessible marker to predict prognosis in liver cirrhosis. The aim of this study to investigate the relationship between plasma CAR and prognosis by Child Pough (CP) also between plasma CAR and fibrosis by APRI and FIB-4 score in cirrhotic patients.</p><p><b><i>Material and method:</i></b> We performed a single-center, observational retrospective study and analyzed to 217 cirrhotic patients were admitted in the gastroenterohepatology department. The plasma CAR of patients was calculated from blood samples taken at the same time as the scoring examination. The biochemical examination results of three scores (Child Pough, APRI, and FIB-4) were calculated. Correlation between plasma CAR to both scores has been analyzed.</p><p><b><i>Results:</i></b> Spearman correlation analysis showed correlation indicated that there were statistically significant positive relationship between plasma CAR to CP score (r= 0.642, p < 0.001), APRI score (r = 0.776, p <0.01), and FIB-4 score (r=0.674, p= 0.006).</p><p><b><i>Conclusion:</i></b> Plasma Creatinin to Albumin Rasio can be a useful biomarker used for evaluating the prognosis and fibrosis in cirrhotic patients. Further researches are warranted to confirm correlation between Plasma CAR and other invasive or noninvasive liver fibrosis examinations.</p><p><b>PP-02-176</b></p><p><b>Bacteriological Profile And Antibiotic Resistance Patterns In Adult Cirrhotic Patients With SBP</b></p><p><b>Sobin Chaithram Bhaskaran</b><sup>1</sup>, Ramesh Mohanachandran Nair<sup>2</sup>, K George Thomas<sup>3</sup>, Mercy John Idikkula<sup>3</sup> and U B Thushara<sup>4</sup></p><p><sup>1</sup><i>St. Thomas Hospital, Chethipuzha, Chanaganassery, Kottayam, India;</i> <sup>2</sup><i>Mar Sleeva Medicity, Pala, Kottayam, India;</i> <sup>3</sup><i>Pushpagiri institute of medical sciences and research centre, Thiruvalla, Pathanamthitta, India;</i> <sup>4</sup><i>Believers Church medical college hospital, Thiruvalla, Pathanamthitta, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The objective of study was to evaluate the bacteriological profile and Drug resistance patterns of SBP pathogens.</p><p><b><i>Materials and methods:</i></b> A retrospective study was done for a period of 1 year in a tertiary care hospital in Kerala. A total of 32 adult cirrhotic patients, meeting the inclusion and exclusion criteria were grouped into community acquired and nosocomial based on timing of diagnosis of SBP(within 48 hours and >48 hours) and underwent ascitic fluid paracentesis and sample was sent for culture and sensitivity. Data thus obtained was grouped and studied using Chi Square test and analysis was done using IBM-SPSS.</p><p><b><i>Results:</i></b> Out of 32 cases, Gram negative bacteria were more frequent than gram positive bacteria(78.1% vs 21.8%) and 9(28.1%) were classified as multi drug resistant(MDR) bacteria. E.coli was the predominant bacteria(18, 56.2%) of which 5(27.7%) was found to be MDR. Resistance to third generation cephalosporins, quinolones, piperacillin tazobactam and carbapenems were found in 53%, 62.5%,28.1% and 21.8% of cases respectively. MDR bacteria and gram positive bacteria were more common in nosocomial group(45.5%, 36.4%, p = 0.03). Except for Third generation cephalosporins(45.5% vs.57.1%), resistance to quinolones(63.6% vs 61.9%), piperacillin tazobactam(45.5% vs 19%) and carbapenems(36.4% vs 14.3%) were more frequently found in nosocomial group.</p><p><b><i>Conclusions:</i></b> One third of the study population were found to have MDR. Resistance to third generation cephalosporins and quinolones, which is used for empirical antibiotic treatment and prophylaxis of SBP was high. Hence antibiotic use according to local antibiogram should be strictly followed.</p><p><b>PP-02-177</b></p><p><b>Utilization of systemic inflammatory markers as non-invasive prognostic markers for mortality in liver cirrhosis</b></p><p><b>Ma. Althea Kathrine Elinzano</b></p><p><i>East Avenue Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Cirrhosis-associated immune dysfunction encompasses a specific range of immune changes that occur in the context of chronic liver disease. This study aims to determine if CAR, SII, NLR, MLR, and PLR can predict mortality in liver cirrhosis.</p><p><b><i>Materials and methods:</i></b> This is a single-center, retrospective cohort study which included 219 Filipino patients admitted with liver cirrhosis. Descriptive statistics was used to summarize the demographic, biochemical, and clinical characteristics. Area under the receiver operating characteristic curve was used to determine the diagnostic performance of the systemic inflammatory markers to discriminate mortality.</p><p><b><i>Results:</i></b> The CTP, MELD, MELD-Na scores, and systemic inflammatory markers (NLR, MLR, PLR, CAR, SII) were significantly different between the expired and alive groups. The most common etiology for liver cirrhosis was chronic hepatitis B (44.29%), followed by alcoholic liver disease (39.27%). Majority of the expired patients had decompensated liver cirrhosis (81.36%). The leading cause of mortality among the expired group was hepatic encephalopathy (52.64%).</p><p><b><i>Conclusion:</i></b> Overall, systemic inflammatory markers (NLR, MLR, CAR, SII) alone are insufficient to predict mortality. Their combination with established liver scoring systems (NLR-CTP, NLR-MELD, NLR-MELD-Na, MLR-CTP, MLR-MELD, MLR-MELD-Na, CAR-CTP, CAR-MELD, and CAR-MELD-Na scores) may offer additional prognosticating value, highlighting the potential for improved risk stratification and patient management. Among these markers, the CAR-CTP score with a cutoff level of > 12.24 had the highest accuracy (70.78%), sensitivity (71.19%), and specificity (70.30%) in predicting mortality in liver cirrhosis.</p><p><b>PP-02-178</b></p><p><b>Dietary Pattern and Energy Fulfllment among Cirrhosis Patient with Hepatic Encephalopathy and Non Hepatic Encephalopathy</b></p><p><b>Mochamad Anief Ferdianto</b><sup>1</sup>, Juferdy Kurniawan<sup>1</sup> and Hasan Maulahela<sup>2</sup></p><p><sup>1</sup><i>Hepatology Division, Department of Intenal Medicine, Dr Cipto Mangunkusumo National Referral Hospital/Faculty of Medicine Universitas Indonesia, Jakarta Pusat, Indonesia;</i> <sup>2</sup><i>Gastroenterology Division, Department of Intenal Medicine, Dr Cipto Mangunkusumo National Referral Hospital/Faculty of Medicine Universitas Indonesia, Jakarta Pusat, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We would like to investigate both descriptively and analytically the difference of dietary pattern and energy fulfilment among cirrhosis patient with and without hepatic encephalopathy (HE).</p><p><b><i>Material and Methods:</i></b> We conducted a cross-sectional study in Hepatology Polyclinic of dr Cipto Mangunkusumo National Referral Hospital, Jakarta during whole 2023 period. Hepatic encephalopathy diagnosis were made clinically with adjuvant Stroop and Flicker tests. Dietary pattern including carbohydrate, protein, fat, and dietary fiber was determined using 24 hours food recall questionnaire (Indonesia language validated) which the results calculated for energy fulfillment. We compared the median of both groups using Mann-Whitney test to elucidate the difference.</p><p><b><i>Results:</i></b> 86 patients (66 non HE and 20 HE) enrolled in this study. Study subjects mean age was 53 ± 8,10 years with no difference between BMI level (p=0,713) and obesity prevalence in both groups (p=0,801). Energy, protein, fat, carbohydrate, and fiber consumption was found not to be different between HE and non HE patients. (p>0,05). However energy (p=0,003) and carbohydrate (p=0,036) daily requirement fulfillment was found higher in HE compared to Non-HE significantly.</p><p><b><i>Conclusions:</i></b> Absolute consumption of dietary component did not differ between groups. Daily energy and carbohydrate requirement fulfillment was discovered higher in HE group.</p><p><b>PP-02-179</b></p><p><b>Simvastatin in Treatment and Reducing Events of Decompensation in Patients With Liver Cirrhosis: A Meta-Analysis</b></p><p><b>Nicole Ferrer</b>, Miguel Jemuel Cabusas and Ian Homer Cua</p><p><i>St. Luke's Medical Center Bonifacio Global City, Taguig City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To assess the efficacy and use of Simvastatin (Statin) in treatment and reducing events of decompensation in patients with decompensated Liver Cirrhosis. To investigate the effect of Simvastatin in improving liver function. To determine if simvastatin treatment leads to a reduction in portal hypertension, and</p><p>subsequent reduction in events of decompensation. To analyze the effect on patient’s clinical outcomes</p><p><b><i>Materials and Methods:</i></b> A Meta-analysis was done using randomized control trials who applied patients aged 18-75 years old with known liver cirrhosis with portal hypertension using Simvastatin as therapy. Decrease in levels of HVPG (Hepatic Venous Pressure Gradient) or PHI (Portal Hypertension Index) post treatment was used as marker for improvement.</p><p><b><i>Results:</i></b> There was significant difference in HVPG levels and liver function among patients with liver cirrhosis who had adjunct use of simvastatin vs. standard treatment. Visual inspection of the forest plot appears to favor use of simvastatin in improving liver function in patients with liver cirrhosis. A moderate heterogeneity was also observed using the fixed effect model (Chi2 = 4.87, P = 0.08, I2 = 38%).</p><p><b><i>Conclusion:</i></b> While earlier data depict statins to have harmful effects in the liver, evidence, as shown in this paper, suggests that there is positive impact in reducing events of decompensation & portal hypertension. Combining this knowledge and the long track-record of safety and tolerability of statins, we might soon rely on statins to achieve better outcomes in patients with liver cirrhosis without significant additional costs.</p><p><b>PP-02-180</b></p><p><b>Efficacy and Safety of Avatrombopag</b></p><p><b>Rie Goka</b>, Naoki Morimoto, Shunji Watanabe, Mamiko Tsukui, Yoshinari Takaoka, Hiroaki Nomoto, Hiroshi Maeda, Wataru Ueno, Yosuke Otsuka and Kouichi Miura</p><p><i>Division of gastroenterology, Jichi Medical University, Shimotsuke, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Patients with advanced chronic liver disease (CLD) such as cirrhosis often have thrombocytopenia associated with increased splenic function. On the other hand, patients with CLD, including hepatocellular carcinoma may require invasive procedures with the risk of bleeding for examinations and treatments. In these cases, platelet transfusion is sometimes required to reduce the risk of bleeding complications. In 2015, the administration of lusutrombopag became available, and we have experienced cases in which platelet transfusion could be avoided. Avatrombopag, which was newly approved in 2023, is no need to check platelet counts 5 days after administration, so it is considered more useful in clinical setting. In this study, we examined the efficacy and safety of avatrombopag.</p><p><b><i>Methods:</i></b> Eight patients with CLD who received avatrombopag between June 2023 and June 2024 were retrospectively evaluated for serological parameters, Spleen Index, and platelet increase.</p><p><b><i>Results:</i></b> The median Spleen Index was 92.0 cm² (22.1-112.5) and the median platelet count before administration of avatrombopag was 4.9×10³/μL (3.6-5.0). All patients showed an increase in platelets, and platelet counts increased to over 5.0 ×10³/μL, therefore, platelet transfusion avoidance rate was 100%, and no hemorrhagic event occurred.</p><p><b><i>Conclusions:</i></b> Avatrombopag was useful in safely and effectively increasing platelet counts in patients with CLD and thrombocytopenia who were scheduled to undergo invasive procedures. In addition, there is no need to check platelet counts after the administration of avatrombopag, so it is considered to be beneficial in clinical practice use.</p><p><b>PP-02-181</b></p><p><b>Clinical and Paraclinical Features of Decompensated Cirrhosis during COVID-19 Pandemic: A One-Year Single-Center Study.</b></p><p><b>Lyly Heng</b><sup>1</sup>, Channa Sann<sup>1,2</sup>, Ong Chea<sup>1,3</sup>, Vutha Ky<sup>1,2</sup>, Sopheak Mak<sup>1,2</sup>, Sidet Chheang<sup>1,2</sup> and Veasna Moeung<sup>1</sup></p><p><sup>1</sup><i>University Of Health Sciences, Phnom Penh, Cambodia;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Calmette Hospital, Phnom Penh, Cambodia;</i> <sup>3</sup><i>Cambodia-China Friendship Kossamak Hospital, Phnom Penh, Cambodia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> Decompensated cirrhosis, an advanced stage of chronic liver disease, leads to high morbidity and mortality with various complications and significantly reduced life expectancy. This study aims to illustrate and characterize the clinical and paraclinical features of various etiologies of decompensated cirrhosis and its complications, while concurrently assessing any additional comorbidities to optimize diagnostic and management strategies.</p><p><b><i>Methods:</i></b> This retrospective, analytical, single-center study was conducted in the Department of Gastroenterology and Hepatology at Calmette Hospital, involving patients with decompensated cirrhosis over a 12-month period.</p><p><b><i>Results:</i></b> Out of a total 200 decompensated cirrhosis, the mean age of the patients was 58.44 ± 12.219 years, with a male predominance of 65.5% and the six ratio (M/F) of 1.89/1. Among these patients, 29% were from Phnom Penh, while the remaining 71% were from the provinces. The primary reasons for hospitalization included GI bleeding (45%), ascites (23.5%), confusion (8.5%), and other causes (23%). The most common etiologies of cirrhosis were HBV (30%), alcoholism (27%), HCV (25%), HBV-HCV co-infection (5.5%), and other causes (12.5%). During hospitalization, the most frequently observed complications were GI bleeding (77%), ascites without SBP (75%), ascites with SBP (3.5%), HCC (17.5%), and hepatic encephalopathy (13%).</p><p><b><i>Conclusion:</i></b> This study highlights the significant impact of decompensated cirrhosis, primarily in middle-aged males, with major causes being hepatitis B and alcoholism. Key issues include gastrointestinal bleeding and ascites, with severe complications common. The findings emphasize the need for targeted strategies to improve diagnosis, management, and patient outcomes.</p><p><b>PP-02-182</b></p><p><b>Gut Metagenomic Characteristics of Cirrhotic Patients with Gastrointestinal Bleeding</b></p><p><b>教授 Hui Huan</b> and 副教授超刘</p><p><i>西藏自治区人民政府驻成都办事处医院, 成都, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Investigating changes in gut microbiota may aid in developing predictive markers and treatments for Gastrointestinal bleeding (GIB)</p><p><b><i>Methods:</i></b> This study included 26 Tibetan cirrhotic patients, divided into two groups: 13 cirrhotic patients with GIB and 13 cirrhotic patients without GIB. Gut microbiota profiles were analyzed using metagenomic sequencing.</p><p><b><i>Results:</i></b> The gut microbiota composition differed significantly between the two groups. At the genus level, the abundance of Enterobacter, Enterococcus, Lactobacillus, and Clostridium was significantly higher in patients with GIB, while Butyricicoccus, Akkermansia, Bacteroides, and Faecalibacterium were significantly lower. The abundance of Firmicutes showed significant changes, with higher levels of Ruminococcus gnavus, Tyzzerella nexilis, Erysipelatoclostridium ramosum and Bacteroides sp._3_2_5 observed in patients with GIB compared to those without GIB. The beta diversity was significantly lower in patients with GIB (p<0.001). Functionally, the abundance of the ABC-type multidrug transport system, ATPase component, signal transduction histidine kinase, acetolactate synthase large subunit were increased in the GIB group. KEGG pathways such as non-homologous end joining, AGE-RAGE signaling pathway in diabetic complications, hematopoietic cell lineage, cytokine-cytokine receptor interaction, and NF-κB signaling pathway were enriched in the GIB group, while glycolysis/gluconeogenesis, glycine, serine and threonine metabolism, D-alanine metabolism, and epithelial cell signaling in Helicobacter pylori infection were decreased. Spearman correlation analysis showed that hepatic venous pressure gradient was positively correlated with the abundance of Ruminococcus gnavus.</p><p><b><i>Conclusion:</i></b> Cirrhotic patients with GIB exhibit reduced gut microbiota diversity and altered microbial composition. The significantly changed bacterial species may serve as predictive markers or therapeutic targets for gastrointestinal bleeding.</p><p><b>PP-02-183</b></p><p><b>Hepatic sarcoidosis- an unusual cause of jaundice: a case report</b></p><p><b>Md. Musab Khalil</b></p><p><i>Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Sarcoidosis is a chronic disorder. Its hallmark is the presence of noncaseating granuloma. Most cases are asymptomatic. However, few of them progress to liver cirrhosis and liver failure. Treatment of hepatic sarcoidosis is challenging as there is no large randomized controlled till date has been done to evaluate the efficacy of drugs. The Unique characteristic of this case report is that Sarcoidosis may present with jaundice and need to be borne in mind when a patient presenting with jaundice and no usual cause failed to be identify any usual cause. Despite having sarcoidosis, the patient did not have any respiratory symptom.</p><p><b><i>Case presentation:</i></b> We are presenting a case of hepatic sarcoidosis who presented with abdominal pain, fever and jaundice. After excluding of all common causes of jaundice, hepatic sarcoidosis was diagnosed with liver biopsy.</p><p><b><i>Conclusion:</i></b> High levels of suspicion is needed to diagnose hepatic sarcoidosis as this is an unusual cause of jaundice. Although rare, hepatic sarcoidosis can be treated with steroids. The prime take-away message from this case report is to consider Sarcoidosis as an unusual but treatable cause of jaundice when the conventional causes are failed to be the cause of jaundice.</p><p><b>PP-02-184</b></p><p><b>Hepatic multiphase CT with Low-concentration Iodine Contrast (270mg Iodine/mL) using Low Mono-energetic Image</b></p><p><b>Jin Sil Kim</b></p><p><i>Ewha Womans University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to evaluate whether the image quality and detectability of hepatic multiphase CT with low-concentration iodine contrast(270mg Iodine/mL) using a low mono-energetic image(40keV) (LCLM CT) are non-inferior to those of standard CT (high-concentration iodine contrast, 350mg Iodine/mL) using hybrid iterative reconstruction(SDCT) in patients with chronic liver disease focusing on arterial phase</p><p><b><i>Materials and Methods:</i></b> This study included 67 patients with LCLM CT and SDCT. Qualitative and quantitative image noise and contrast were also compared between 2 different CT images. Lesion conspicuity was analyzed using generalized estimating equation analysis, and the detectability of hepatic arterial focal lesions was compared using the Jackknife free-response receiver operating characteristic analysis.</p><p><b><i>Results:</i></b> Mean overall arterial phase image quality scores with LCLM CT and SDCT were 4.90 ± 0.39 and 4.94 ± 0.24, showing noninferiority (difference: -0.045; 95 %CI, -0.123 to 0.034). Qualitative noise, contrast, and hepatic artery clarity of the two CT images were also not significantly different(p >0.05). Contrast-to-noise ratio of the aorta was higher in LCLMCT than in SDCT (71.8 ± 12.6 vs 46.6 ± 14.9 p <0.0001 ). The comparative analysis demonstrated that lesion conspicuity was slightly higher on SDCT than on LCLM CT images without statistical significance (P= 0.195). The figure of merit for detectability of arterial hepatic focal lesion was 23 for LCLMCT and 22 for SDCT, showing noninferiority (difference: - 0.0217, 95 %CI: –0.0666 to 0.0232).</p><p><b><i>Conclusion:</i></b> LCLM CT showed non-inferior overall image quality and detectability of arterial focal hepatic lesions compared to SDCT</p><p><b>PP-02-185</b></p><p><b>The Impact of Fecal Bifidobacterium/Enterobacteriaceae Ratio on 90 days Hospitalization and Mortality in Liver Cirrhosis</b></p><p><b>Cokorde Istri Yuliandari Krisnawardani K</b><sup>1</sup>, Komang Agus Wira Nugraha<sup>2</sup>, Ketut Mariadi<sup>2</sup> and Gde Somayana<sup>2</sup></p><p><sup>1</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Denpasar, Indonesia;</i> <sup>2</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The imbalance of gut microbiota in liver cirrhosis patients, particularly a decreased Bifidobacterium and Enterobacteriaceae (BE) ratio, has been linked to potentially worsening liver inflammation and related complications. The specific impact of changes in this ratio on short-term outcomes such as 90-day mortality and hospitalization in liver cirrhosis patients is unclear. This study aimed to explore the relationship between fecal BE ratio and these critical outcomes.</p><p><b><i>Materials and Methods:</i></b> This single-center prospective cohort study involved liver cirrhosis patients. Individuals with recent gastrointestinal bleeding, use of antibiotics, systemic infection, or gastrointestinal infection were excluded. Fecal Bifidobacterium and Enterobacteriaceae (CFU/g) were examined using DNA isolation, and BE ratios were calculated. The ratio was categorized as decreased (<0.0184 CFU/g) or normal-increased (≥0.0184 CFU/g). Subjects were followed for 90 days, and hospitalization and mortality were recorded.</p><p><b><i>Results:</i></b> Eighty patients (70% male) with a median age of 54 years were enrolled. The cumulative 90-day hospitalization and mortality rates were 30% and 17.5%, respectively. The Kaplan-Meier curve revealed no significant difference in mean survival time between subjects with decreased and normal-increased BE ratios (p=0.595). In Cox Regression analysis, a decreased BE ratio was not associated with hospitalization (HR: 1.160, 95% CI 0.512-2.630, p=0.722) or mortality (HR: 1.096, 95% CI 0.378-3.180, p=0.866), but worse liver function (Child-Pugh score ≥7) was associated with hospitalization (HR: 3.648, 95% CI 1.368-9.950, p=0.010) and mortality (HR: 5.289, 95% CI 1.173-23.83, p=0.030).</p><p><b><i>Conclusion:</i></b> In this study, decreased BE ratios were not associated with 90-day hospitalization and mortality in liver cirrhosis patients.</p><p><b>PP-02-186</b></p><p><b>Serum fibrinogen vs INR in predicting rebleed and mortality in cirrhotic patients</b></p><p><b>Rishikesh Malokar</b>, Sanjay Chandnani, Shubham Jain and Pravin Rathi</p><p><i>Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> Predicting and managing bleeding in cirrhotic patients has remained challenging.</p><p>No single parameter can accurately predict future bleeding risk and prognosis. The aim of the study is to evaluate the role of fibrinogen level and INR in patients with cirrhosis with bleeding to predict rebleeding risk, hospitalization, and mortality.</p><p><b><i>Method:</i></b> A prospective observational study. Interim analysis of 84 cirrhotic patients who presented with bleeding. Assessment of association of low fibrinogen (<120mg/dl) and high INR (>1.5) with the first bleeding episode, rebleeding, admission, and mortality. Patients were followed up for 1 year.</p><p><b><i>Results:</i></b> Out of 84 patients,16 had low fibrinogen (<120mg/dl), and 28 patients had an INR >1.5. In the low fibrinogen group(N=16), 9 (56.25%) had rebleeding,8 (50%) had repeated admission, and 7 (43.75%) patients died. In the high INR group (N=28), 12(42.85%) had rebleeding, 11(39.28%) needed repeated hospitalization, and 13(46.42%) died. The difference between both groups for rebleeding episodes (p =0.09), repeated admissions (p=0.11), and mortality (p=0.91) at 1 year was not statistically significant.</p><p><b><i>Conclusion:</i></b> There is no difference between low fibrinogen and raised INR as a predictor of rebleeding episodes and future hospitalization and mortality in patients with cirrhosis. In this interim analysis, although the p-value of rebleeding rates was insignificant, there is a trend in numbers supporting low fibrinogen as a risk factor.</p><p><b>PP-02-187</b></p><p><b>The Value Of Albi And Albi-Plt Score In Predicting Esophageal Varices In Cirrhotic Patients</b></p><p><b>Thang Nguyen Dinh</b></p><p><i>Cho Ray Hospital, Ho Chi Minh City, Viet Nam</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to describe the prevalence of esophageal varices (EVs) in liver cirrhosis patients and to evaluate the accuracy of the ALBI and ALBI-PLT scores in predicting the presence of EVs in these patients.</p><p><b><i>Materials and Methods:</i></b> We conducted a cross-sectional study that enrolled 152 cirrhotic patients from 11/2022 to 5/2023 at the Gastroenterology Department of Cho Ray Hospital.</p><p><b><i>Results:</i></b> The study population consisted of 152 cirrhosis patients, with 100 males and 52 females, ranging in age from 28 to 87 years (mean: 56 years). The prevalence of EVs in cirrhotic patients is 56%. The ALBI score has moderate predictive ability for the risk of having EVs (AUC = 0.78), as well as for the risk of varices needing treatment (VNT) (AUC = 0.79). The ALBI-PLT score also demonstrates moderate predictive ability for the risk of having EVs (AUC = 0.76) and VNT (AUC = 0.77). The cutoff threshold for the ALBI score in predicting EVs in cirrhotic patients is -2.76, with sensitivities and specificities being 96.5% and 90.3%, respectively. An ALBI score below -2.76 would have spared 20.4% of patients from EGDs, missing 9.7% of VNT. For the ALBI-PLT score, the cutoff threshold in predicting EV in cirrhotic patients is 2, with sensitivities and specificities being 96.5% and 87%, respectively. An ALBI score below 2 would have spared 15.8% of patients from EGDs, missing 4.8% of VNT.</p><p><b><i>Conclusion:</i></b> The ALBI and ALBI-PLT scores are reliable predictors of esophageal varices in patients with liver cirrhosis.</p><p><b>PP-02-188</b></p><p><b>Ascites associates with exacerbation after endoscopic treatment for esophageal varices</b></p><p><b>Akihiko Nishimura</b> and Akio Moriya</p><p><i>Mitoyo Jeneral Hospital, Kannonji, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Esophageal varices, one of the complications of cirrhosis, sometimes treated endoscopically, often recur and require re-treatment. We investigated factors associated with exacerbations.</p><p><b><i>Materials and Methods:</i></b> Forty-four patients treated endoscopically at our institution since 2014 were analyzed regarding the endpoint of exacerbation requiring re-treatment, rupture, or death, and the associated factors using a Cox proportional hazards model.</p><p><b><i>Results:</i></b> The patients were 57% male, median age 69 years. There were 27% with alcoholic liver injury, 5% with hepatocellular carcinoma, 30% with HCV infection, 5% with HBV infection, 9% with autoimmune hepatitis, 18% with primary biliary cholangitis, and 16% with fatty liver as complications.</p><p>Exacerbation of varices and death were combined in 29 cases (66%), of which 16 were deaths.</p><p>In univariate analysis, ascites effusion and Child-Pugh score were significant factors, while multivariate analysis with these factors showed that a small or large amount of ascites effusion was a significant independent factor (hazard ratio, 2.394; 95% confidence interval, 1.0206 - 5.614).</p><p><b><i>Conclusion:</i></b> Ascites was an exacerbating factor after endoscopic treatment for esophageal varices.</p><p><b>PP-02-189</b></p><p><b>Frequency of Minimal Hepatic Encephalopathy in Cirrhotic patients Attending a Tertiary Level hospital</b></p><p><b>MD Nuruzzaman</b><sup>1</sup> and Majharul Haq<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;</i> <sup>2</sup><i>Department of Gastroenterology, Dhaka Medical College, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Minimal hepatic encephalopathy (MHE) is defined as patients with normal mental and neurological examinations but with several neuropsychiatric and neurophysiological defects identified by psychometric tests. Minimal hepatic encephalopathy (MHE) has implications for health-related quality of life as well as for survival of cirrhotic patients. This study intends to investigate the frequency of minimal hepatic encephalopathy in cirrhotic patients in a tertiary level hospital in Bangladesh.</p><p><b><i>Methods:</i></b> This was an observational cross-sectional study done in BIRDEM General Hospital from August 2013 to July 2014. Eighty- five patients were selected by non-random sampling. Demographic, clinical and biochemical data were obtained. Cognitive functions were tested by using Bangla adaption of mini mental state examination (BAMSE) to ensure normal mental and neurological state AND psychometric tests- Number connection tests-A (NCT-A) and Digit Symbol Test (DST) were done for diagnosis of MHE.</p><p><b><i>Results:</i></b> The total number of study patients was 85, among then 52 were male and 33were female. Mean age of the patients 55.78(+ 10.30) years. The frequency of MHE was 64.7%. MHE varied with different Child Turcot Pugh scores (CTP-B-35.2% and CTP-C -72.05%, P= 0.005). There was significant difference between MHE positive and negative groups in terms of mean platelet count, prothrombin time, serum ammonia, albumin and sodium level.</p><p><b><i>Conclusions:</i></b> Cirrhotic patients were found to have a high frequency of MHE that is proportionate to the degree of liver function. Proper management should be implemented to avoid MHE among cirrhotic patients.</p><p><b>PP-02-190</b></p><p><b>APRI and FIB-4 Index in the Diagnosis of Chronic Liver Disease: An Analytical Cross-sectional Study</b></p><p><b>Federico Iv Peralta</b>, Marc Jusenn Yumena, Sarah Jean Bellido and Juliet Cervantes</p><p><i>St. Luke's Medical Center -Quezon City, Metro Manila, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Chronic liver disease (CLD) involves progressive liver function deterioration, with late-stage liver cirrhosis characterized by fibrosis and architectural distortion, often necessitating transplantation. While early-stage reversibility exists, most advanced cases are irreversible. Liver elastography is a valuable noninvasive diagnostic tool, but its limited availability in resource-constrained areas necessitates alternative fibrosis scoring systems. This study aims to assess the accuracy of the Aspartate Aminotransferase to Platelet Ratio Index (APRI) and Fibrosis-4 Index (FIB-4) in diagnosing CLD among adults with suspected or confirmed CLD.</p><p><b><i>Materials and Methods:</i></b> This observational, analytical cross-sectional study included adult patients (≥18 years) with suspected or confirmed CLD who underwent liver elastography at St. Luke’s Medical Center, Quezon City, from January 2015 to November 2022. The study analyzed 208 samples to compare APRI and FIB-4 scores against liver elastography results.</p><p><b><i>Results:</i></b> The Receiver Operating Characteristic (ROC) curve revealed that FIB-4 (AUC 0.76) had a superior discriminatory ability for predicting F4 fibrosis compared to APRI. The optimal APRI cut-off for predicting F4 fibrosis was >0.5835 (accuracy 71.64%, sensitivity 77.78%, specificity 66.06%), while the optimal FIB-4 cut-off was >3.117 (accuracy 75.48%, sensitivity 67.78%, specificity 82.57%). Both APRI and FIB-4 scores were positively correlated with liver elastography METAVIR stages.</p><p><b><i>Conclusion:</i></b> Although APRI and FIB-4 have limitations in accurately predicting advanced fibrosis, their positive correlation with liver elastography METAVIR stages makes them useful tools for identifying individuals at risk of advanced liver fibrosis or cirrhosis, aiding in the prioritization of further evaluation and intervention.</p><p><b>PP-02-191</b></p><p><b>Comparison of LMR with CPS and MELD Score Determining Severity and Outcome in Cirrhotic Patient</b></p><p><b>Mohammad Mahatabur Rahman</b></p><p><i>Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Objectives:</i></b> To evaluate the neutrophil-to-lymphocyte ratio (NLR) and lymphocyte-to-monocyte ratio (LMR) for the prediction of prognosis of liver cirrhosis patients.</p><p><b><i>Material and methods:</i></b> This Cross-sectional analytical study was conducted in Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from July 2020 to December 2020. Total 40 compensated cases and 40 decompensated cases were included and information regarding clinical profile, laboratory parameters was collected. LMR, NLR, MELD score and CP score were calculated both groups. Correlation between LMR, NLR and CP/MELD score were established and cut-off values of LMR and NLR were obtained.</p><p><b><i>Results:</i></b> The mean value of NLR was significantly higher in decompensated cirrhosis patients than that of compensated ones and LMR was significantly higher in compensated cirrhosis patients(p<0.001). NLR had a significant strong positive correlation with CPS scores (r=0.774, p<0.05) whereas LMR had a significant negative correlation with CPS scores (r = -0.732, p<0.05). At a cut-off value ≥5.61, NLR had 80% sensitivity and specificity & LMR had 64% sensitivity & 79% specificity, cut-off value was found ≤ 2.45. A cut-off value of MELD score ≥ 26.5 was 100% sensitive but 80% specific in the prediction of death among patients with cirrhosis. Those parameters were capable of presaging the early prognosis of liver cirrhosis patients. Of them, NLR was found most precise.</p><p><b>PP-02-192</b></p><p><b>A study of the profile of infections in the hospitalized patients with cirrhosis of liver.</b></p><p><b>Rhimanshu Soni</b>, Preetam Nath and A.C Anand</p><p><i>Department of Gastroenterology & Hepatology, Kalinga Institute Of Medical Sciences, Kushabhadra Campus, KIIT University, 751024, Bhubaneswar, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Bacterial infections are common in patients with liver cirrhosis. Infections worsen the outcomes in terms of organ failures, length of hospital stay & mortality. We here aimed to study the profile and impact of bacterial infections on outcomes in terms of 28 days mortality, length of hospital stay & organ failure in patients with liver cirrhosis.</p><p><b><i>Methods:</i></b> The study subjects were inpatients with cirrhosis of liver in KIMS Bhubaneswar from September 2022 to February 2024. All the demographic, clinical, microbiological & treatment data were collected at the time of admission. Patient were followed up until death or for 28 days from the day of admission.</p><p><b><i>Results:</i></b> A total of 200 patients with cirrhosis of liver were enrolled in this study out of which 128 had infection (64%). Most frequent infection were UTI (46.1%) followed by SBP(25%), spontaneous bacteremia (14.1%) & others 14.8%. Microbiological sampling was performed and culture-positive results were obtained in (n=115/200, 57.5%). Among them E.coli was the most frequent organism isolated (n=47/115,40.86%). Multi drug-resistant (MDR)-bacteria were found in (n=59/115, 51.3%). 28 days mortality (33.6% vs 13.8% p=0.002), length of hospital stay (9.57vs7.99days, p=0.046) were significantly higher in patients with infections than those without. Among all organ failures, renal, circulatory & respiratory failure were significant more in patient with infection(p<0.05).</p><p><b><i>Conclusion:</i></b> The present study showed high prevalence of bacterial & MDR infection in patient with cirrhosis of liver. Patients with infections had higher 28 days mortality, length of hospital stay & organ failures.</p><p><b>PP-02-193</b></p><p><b>Long-term outcomes of L-carnitine Administration in Hyperammonemia Patients with Liver Cirrhosis</b></p><p><b>Joji Tani</b>, Kei Takuma, Kazutoshi Fuke, Asahiro Morishita and Hideki Kobara</p><p><i>Faculty Of Medicine, Kagawa University, Miki-cho, Kagawa, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There are few data on the long-term efficacy of L-carnitine administration in improving blood ammonia concentration (BAC) and preventing recurrence of hepatic encephalopathy (HE). This study aimed to determine the effects of long-term L-carnitine administration on BAC and HE.</p><p><b><i>Methods:</i></b> Of 444 patients with L-carnitine administration from April 2012 to March 2021, we enrolled 242 patients with hyperammonemia or HE. A multicenter retrospective study was conducted to determine the long-term efficacy of L-carnitine administration.</p><p><b><i>Results:</i></b> Median BAC at the start and at 12, 24, 48, 96,144, and 192 weeks was 123, 95.5, 88, 83, 96, 82, and 86 μg/dL, respectively. BAC were significantly lower than those at the start (p<0.05, respectively). The median improvement time to normalization of BAC was 100 days. The 113 patients with a history of hospitalization for HE followable for 2 years before and after L-carnitine therapy had a total of 183 hospitalizations for HE before treatment, compared with 62 hospitalizations after treatment (p<0.001). The event incidence rate due to HE, such as emergency hospitalization, emergency transport, or additional medications, at 60, 180, 360, 720, and 1080 days after L-carnitine administration was 5.7%, 16.9%, 20.3%, 29.5% and 38.5%, respectively. The change in MELD scores before and after L-carnitine administration was statistically significant, while other variables (liver reserve function, nutritional status, and muscle index) did not show significant changes. Finally, the median survival time was 880 days.</p><p><b><i>Conclusions:</i></b> Long-term L-carnitine administration is effective in hyperammonemic patients, and can reduce hospitalizations and events due to HE.</p><p><b>PP-02-194</b></p><p><b>Gut Akkermansia muciniphila mitigates hepatic fibrosis by modulating riboflavin metabolism through the gut-liver axis</b></p><p>Lu Zhang and <b>Cui-Hua Lu</b></p><p><i>Department of Gastroenterology, Afliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Akkermansia muciniphila (AKK), a gut bacterium, is gaining recognition for its potential in treating metabolic disorders, including its ability to combat liver fibrosis.</p><p><b><i>Materials and Methods:</i></b> In this study, we employed a CCl4-induced hepatic fibrosis mice to explore the mechanisms of AKK in intervening hepatic fibrosis. Mice treated with AKK showed effective reversal of hepatic fibrosis, including collagen deposition, inflammation, and hepatic injury, which was attenuated in hepatic fibrosis mice treated with antibiotics.</p><p><b><i>Results:</i></b> The therapy enhanced oxidative stress and vitamin metabolism along the gut-liver axis. This led to the deactivation of hepatic stellate cells and a modification in gut microbiota composition, alongside an increase in riboflavin transport from the gut to the liver. These metabolic improvements occurred with an increase in riboflavin levels transported from the gut to the liver. Riboflavin displayed similar beneficial metabolic effects in vitro and in mice, effectively ameliorating hepatic fibrosis. Riboflavin itself showed promising effects, mirroring AKK's benefits by alleviating hepatic fibrosis.</p><p><b>PP-02-195</b></p><p><b>Safety And Efficacy Of Lenvatinib And Sorafinib In Patients With Advanced Hepatocellular Carcinoma.</b></p><p>Nazish Butt<sup>1</sup> and <b>Gulzar Khan</b><sup>2</sup></p><p><sup>1</sup><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan;</i> <sup>2</sup><i>JPMC, Karachi, Pakistan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Sorafenib has been the standard targeted therapy for advanced HCC, but recent studies suggest Lenvatinib might be a more promising option. This study aimed to compare the safety and efficacy of Lenvatinib and Sorafenib in treating patients with advanced HCC.</p><p><b><i>Materials and Methods:</i></b> This was a prospective study our institution between August 2023 to April 2024. Patients were divided into two groups based on the targeted therapy received: Sorafenib or Lenvatinib. The primary outcome was overall survival. Adverse events (AEs) were assessed within 6 weeks of treatment initiation using common terminology criteria for AEs version 4.0.</p><p><b><i>Results:</i></b> A total of 36 patients were included in the study. Twenty patients received Sorafenib and sixteen received Lenvatinib. The two groups showed no significant differences in baseline characteristics after propensity score matching. Lenvatinib was associated with a longer overall survival time three months more then that of sorafenib group(p-value <0.0032).The most common AEs in the sorafenib group were loss of appetite (66%),fever (50.0%), diarrhea (45%) and hand-foot skin reaction (30%). In contrast, the most common AEs in the lenvatinib group were loss of appetite (55%), hypertension (24%), hand-foot skin reaction (18%), and proteinuria (20%).</p><p><b><i>Conclusions:</i></b> This study suggests that Lenvatinib may be a more effective treatment option compared to Sorafenib for patients with advanced HCC. Lenvatinib treatment resulted in significantly better objective response rates and overall survival. Sorafenib and Lenvatinib exhibit distinct AE profiles in patients with advanced HCC.</p><p><b>PP-02-196</b></p><p><b>Extrahepatic recurrence after surgical resection of hepatocellular carcinoma without intrahepatic hepatocellular carcinoma: Multi-institutional observational study</b></p><p><b>Sanghyuk Jung</b></p><p><i>Chonnam National University Hospital, Gwangju, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Extrahepatic recurrence (EHR) is a known poor prognostic factor for hepatocellular carcinoma (HCC). While EHR can occur in high-risk patients post-surgery, its characteristics without concurrent intrahepatic HCC are less understood. This study examines the clinical features and risk factors for EHR without remnant intrahepatic HCC at diagnosis.</p><p><b><i>Materials and Methods:</i></b> From January 2004 to December 2019, 1,069 treatment-naive patients who underwent curative hepatectomy for HCC at four tertiary academic hospitals were assessed. After excluding those with intrahepatic recurrence (IHR) or combined EHR and IHR, and those with insufficient clinical records, 569 patients were enrolled. The median follow-up duration was 3.91 years. Multivariate analysis via Cox regression identified variables associated with EHR.</p><p><b><i>Results:</i></b> Thirty-eight patients developed EHR without remnant intrahepatic HCC during a median follow-up of 1.04 years. EHR patients experienced significantly earlier initial HCC recurrence than those without EHR (1.73 vs. 4.43 years). Multivariate analysis revealed that EHR patients had higher venous and/or lymphatic involvement (HR 2.418, p=0.020), tumor necrosis (HR 2.592, p=0.009), and higher initial tumor stages beyond the Milan criteria (HR 3.008, p=0.001). EHR was strongly associated with poor survival (HR 14.044, p<0.001). The cumulative rates of EHR and survival correlated with the number of risk factors.</p><p><b><i>Conclusion:</i></b> EHR without remnant viable HCC can occur in many patients post-surgical resection. Close monitoring for EHR is essential in this high-risk group, even without evidence of intrahepatic HCC.</p><p><b>PP-02-197</b></p><p><b>The Clinical Features and Mortality of Hepatocellular Carcinoma associated Hepatitis B Patients in Tertiary Hospital</b></p><p><b>Ulfa Kholili</b>, Kartika Wensdi Renantriandani, Vembi Rizky Rahmawati, Febrian Ramadhan Pradana, M Alwi Alaydrus, Awwaliyah Azmi Izzati, M Zulfikar Defianto, Poernomo Boedi Setiawan and Muhammad Miftahussurur</p><p><i>FK Unair-RSUD Dr. Soetomo, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives and Aim:</i></b> Hepatocellular carcinoma (HCC) is considered a significant burden, and its associated rate of mortality is increasing. The aim of this study to observe clinical features and mortality of HCC associated Hepatitis B.</p><p><b><i>Material and methods:</i></b> An observational study with cohort prospective, total sampling method, follow up January 2023 to July 2024, descriptive data analyzing.</p><p><b><i>Result:</i></b> This study involved 129 HCC related Hepatitis B patients consist of 82.9% male, 76% Javanese ethnic and average age 51.86 y.o. The most frequent clinical manifestation were abdominal pain (82.2%) and hepatomegaly (82.9%), comorbid hypertension (20.9%), CTP score A (45.7%), and presented BCLC staging consecutively BCLC-A (4.7%), BCLC-B (41.1%), BCLC-C (34.1%), and BCLC-D (20.2%). The tumor characteristics were single nodule (52.7%), right lobe (54.3%), presence of portal vein thrombus (37.2%), tumor size 11.9 cm. Laboratory findings were Hb 12.13, Albumin 3.34 mg/dL, ALBI Score -1.89 (grade 2), and the median of thrombocyte 260.000, Bilirubin 1.30 mg/dL, SGOT 123u/L, SGPT 49u/L, AFP 3,450 ng/ml, and HBV DNA 1.8 x 10^4 IU/mL. During 18 months observation, 29 (22.4%) patients were found died, with survival rate was 4.8 months, which duration of mortality less than 6 months (79.3%), during 6-12 months (17.2%), more than 12 months (3.4%) were observed. The most cause of mortality was liver failure.</p><p><b><i>Conclusion:</i></b> The mortality of HCC related Hepatitis B was high and the survival rate was 4.8 months. This result similar with other studies of HCC with any different etiologies.</p><p><b>PP-02-198</b></p><p><b>The Efficacy and Safety of Atezolizumab and Bevacizumab Combination Therapy for Advanced Hepatocellular Carcinoma</b></p><p><b>Ji Hoon Kim</b> and Yang Jae Yoo and Eunho Choi and Kwan Soo Byun</p><p><i>Korea University Guro Hospital, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background & Aims:</i></b> Atezolizumab-bevacizumab combination therapy has been approved for first-line systemic chemotherapy in advanced hepatocellular carcinoma. Real-world retrospective analysis was performed to evaluate the effectiveness and safety of the chemotherapy.</p><p><b><i>Methods:</i></b> A single-center, retrospective cohort study was performed. Between January 2020 and July 2023, patients with advanced hepatocellular carcinoma who received atezolizumab-bevacizumab systemic therapy were enrolled. Response to the therapy was assessed with mRECIST criteria. Evaluation of first radiologic response, best overall response, progression-free survival, and overall survival was done. Safety data were collected. Also, prognostic factors for first radiologic response, progression-free survival, and overall survival were also assessed.</p><p><b><i>Results:</i></b> A total of 70 patients were investigated. Baseline characteristics showed a median age of 63 years, male dominance (89%), and viral etiology (84%). Four patients achieved complete response and 19 reached partial response when evaluated by best overall response. Response duration was 4.7 months (range 1.5-25.5). Median progression-free survival was 4.1 months (95% CI, 3.7-6.7), and median overall survival was 24.6 months (95% CI, 11.6-). Age and tumor extent of over 50% of the liver was predictive of progression-free survival (p=0.009 and p=0.007, respectively) Tumor extent of over 50% of the liver and ALBI score were predictive of overall survival (p=0.025 and p=0.004, respectively).</p><p><b><i>Conclusion:</i></b> Atezolizumab-bevacizumab therapy showed valuable outcomes and tolerable safety profiles in real-world advanced HCC patients. The outcome and safety results were comparable to previous studies.</p><p><b>PP-02-199</b></p><p><b>The Role of Glucose Transporters in Tumor Recurrence and Progression in HCC Patients Undergoing TACE</b></p><p><b>Hui-pu Liu</b></p><p><i>Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> We investigates the role of glucose transporters (GLUTs) in hepatocellular carcinoma (HCC) progression, particularly in relation to transarterial chemoembolization (TACE) treatment. We explore the metabolic phenomenon of aerobic glycolysis in HCC, mechanisms of GLUTs-related tumorigenesis, and the influence of hypoxia on tumor behavior.</p><p><b><i>Methods:</i></b> Using the GSE104580 dataset from the Gene Expression Omnibus, we compared gene profiles between TACE responders and non-responders, identifying significant differences in GLUT1, Gal-3, and CD133. We enrolled HCC patients undergoing surgery or TACE, with 16 surgery cases and 4 TACE cases. Tumor specimens were analyzed for markers such as HIF-1α, GLUT1, Gal-3, and CD-133. In cell line experiments, Hep3B and SNU-387 were studied under hypoxic (1% O2) and normoxic (21% O2) conditions, and the GLUT1 inhibitor (BAY-876) was tested.</p><p><b><i>Results:</i></b> Significant differences in GLUT1, Gal-3, and CD133 expression were observed between TACE responders and non-responders. Tumor tissues had higher levels of HIF-1α, GLUT1, Gal-3, and CD-133 compared to non-tumor tissues. Under hypoxia, Hep3B and SNU-387 exhibited increased GLUT1 and HIF-1α expression. Hep3B's cell viability increased under hypoxia, while SNU-387's viability exceeded 200% by 48 hours. IC50 values for BAY-876 varied under normoxia and hypoxia.</p><p><b><i>Conclusion:</i></b> TACE-induced hypoxia increases GLUT1 and cancer stemness markers, potentially accelerating tumor growth and contributing to poor outcomes. GLUT-1 inhibitors may suppress liver cancer cell growth in hypoxic environments, offering a potential solution for TACE resistance. This research highlights the importance of GLUTs in HCC progression and their potential as therapeutic targets, aiming to improve treatment strategies and patient outcomes.</p><p><b>PP-02-200</b></p><p><b>Evaluating the Prognostic Utility of the Geriatric Nutritional Risk Index in Intermediate-Stage Hepatocellular Carcinoma Patients</b></p><p><b>Imelda Maria Loho</b><sup>1</sup>, Kevin Tjoa<sup>2</sup>, Lianda Siregar<sup>1</sup>, Agus Waspodo<sup>1</sup>, Irsan Hasan<sup>2</sup> and Rino Alvani Gani<sup>2</sup></p><p><sup>1</sup><i>Dharmais National Cancer Center, Indonesia;</i> <sup>2</sup><i>Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The geriatric nutritional risk index (GNRI), which is based on body weight, height, and albumin level, serves as a prognostic indicator in various diseases beyond elderly patients. However, its role in hepatocellular carcinoma (HCC) patients remains unclear.</p><p><b><i>Materials and Methods:</i></b> A retrospective cohort study was conducted at two tertiary hospitals in Jakarta, Indonesia. Patients diagnosed with intermediate-stage HCC that allowed GNRI calculation from January 2015–December 2022 were included. Analysis on one-year overall survival (OS) was conducted using the Kaplan-Meier method, and p-values were derived from the log-rank test. Stratification was based on sex, treatment, cirrhosis, Child-Pugh class, alpha-fetoprotein (AFP), and up-to-7 status.</p><p><b><i>Results:</i></b> Of the 125 patients, 61.6% were considered low-risk (GNRI ≥ 92). The one-year overall survival (OS) was 72.8% (low-risk: 77.9% vs. high-risk: 64.6%), with a mean OS of 293 days (95% CI: 273–315). The low GNRI group had a mean OS of 308 days (95% CI: 284–333), with the median OS not reached. Conversely, those with a high GNRI had a mean OS of 268 days (95% CI: 229–307) and a median OS of 331 days. There were no significant survival differences within the overall cohort (p = 0.068), nor within any strata, except for patients with AFP >200 ng/mL (mean OS 329 vs. 236 days; p = 0.002).</p><p><b><i>Conclusion:</i></b> There are no differences in survival between low and high-risk nutritional status in HCC patients based on GNRI. Using GNRI for prognostication may be suitable for specific populations, such as patients with AFP > 200 ng/mL.</p><p><b>PP-02-201</b></p><p><b>Artificial Intelligence in the Prediction of Trans-arterial Chemoembolization Outcomes pre-procedure: An Updated Systematic Review</b></p><p><b>Kai Yi Benjamin Nah</b><sup>1,2</sup>, Elina Cho<sup>2</sup>, Michelle Law<sup>2</sup>, Jia Hao Law<sup>4</sup>, Alfred Wei Chieh Kow<sup>2,3,4</sup>, Cheng Han Ng<sup>1</sup>, Mark Muthiah<sup>1,2,3</sup> and Daniel Qingyao Huang<sup>1,2,3</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Department of Medicine, National University Hospital, Singapore;</i> <sup>2</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore;</i> <sup>3</sup><i>National University Centre for Organ Transplantation, National University Health System, Singapore;</i> <sup>4</sup><i>Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, National University Hospital, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Intermediate stage HCC is a heterogenous group with varying tumor characteristics and responses to trans-arterial chemoembolization (TACE). The BCLC staging system currently uses radiologist-reported tumor characteristics to determine TACE suitability such as tumor size and number, presence of portal blood flow. However, there is a need for more precise methods to predict TACE response to improve patient selection and post-TACE management. This systematic review assesses the performance of AI-based models, including radiomics, in predicting TACE outcomes in HCC patients and evaluates their potential clinical application.</p><p><b><i>Materials and Methods:</i></b> A comprehensive search was conducted in Medline and Embase databases for articles on AI predicting HCC response to TACE from inception to April 7, 2024. Studies were analyzed for their predictive model's effectiveness.</p><p><b><i>Results:</i></b> The review included 64 articles with 13,412 TACE-treated patients. These studies used various imaging modalities, primarily CT and MRI, to develop predictive models with machine learning (ML) and deep learning (DL) algorithms. Results indicate that AI-based models, particularly those using CT imaging, show high predictive value for TACE outcomes, with some models achieving area under the curve (AUC) values above 0.90. Models incorporating radiomics—automatically extracted features from medical images—outperformed those relying on manual measurements by radiologists. Some models also integrated clinical and laboratory data, enhancing predictive accuracy.</p><p><b><i>Conclusion:</i></b> AI-based models show potential in improving TACE outcome predictions, aiding in patient selection and management. However, further validation in diverse clinical settings is needed to confirm these results and facilitate AI integration into routine clinical practice.</p><p><b>PP-02-202</b></p><p><b>Initial Presentation of Hepatocellular Carcinoma as a Hepatic Cyst with Peritoneal Metastases</b></p><p><b>Tatag Primiawan</b></p><p><i>Gadjah Mada University, Magelang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Cystic hepatic lesions are common and range from benign to potentially lethal conditions. Distinguishing between malignant and benign cystic lesions is challenging, even with advanced imaging. Cystic degeneration is a rare manifestation of hepatocellular carcinoma (HCC), making diagnosis difficult without microscopic biopsy examination.</p><p><b><i>Case Illustration:</i></b> A 57-year-old man presented to the Emergency Room with hematemesis, abdominal pain, vomiting, increasing abdominal distension, and weight loss over 4 months. He had no history of hepatitis, hepatotoxin exposure, or alcohol abuse but had a history of repeated transfusions. Physical examination revealed abdominal tenderness, meteorism, and ascites. Tests for chronic liver disease were negative, and ultrasonography showed a non-cirrhotic liver. Barium follow-through revealed a gastric ulcer. MSCT Abdomen showed hepatomegaly with a hepatic cyst and multiple lymphadenopathy. Esophagogastroduodenoscopy revealed a giant gastric ulcer with mucosal dysplasia, and biopsy showed mild chronic gastritis. During laparotomy, biopsies from the omentum, appendix, peritoneal wall, liver, and mesentery indicated high-grade carcinoma metastasis. Immunohistochemistry confirmed primary hepatocellular carcinoma of the liver with metastasis to the omentum, peritoneal wall, mesentery, and appendix.</p><p><b><i>Conclusion:</i></b> Hepatocellular carcinoma (HCC) can rarely present as cystic lesions without liver cirrhosis. Proposed mechanisms for these cystic changes include arterial thrombosis, inflammation, and rapid tumor growth, though the exact cause remains unclear. Some hypothesize immune-related mechanisms involving interleukin-18 inducing interferon-γ release from T cells and natural killer cells, leading to tumor necrosis. Pathological evidence shows an inflammatory response with rapid leukocyte infiltration and massive lymphoid infiltration.</p><p><b><i>Keyword:</i></b> hepatocellular carcinoma, hepatic cyst, peritoneal metastasis.</p><p><b>PP-02-203</b></p><p><b>Demographic and Clinicopathologic Factors of Patients with Hepatocellular Carcinoma at Tertiary Hospital in Indonesia</b></p><p><b>Febry Rahmayani</b> and Suyata Suyata</p><p><i>Mohammad Hoesin General Hospital Palembang/Faculty of Medicine Sriwijaya University, Palembang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The purpose of this study is to characterize the current state of hepatocellular carcinoma (HCC) at Mohammad Hoesin General Hospital Palembang, Indonesia.</p><p><b><i>Materials and Methods:</i></b> This is a retrospective study involving 98 patients, done at Mohammad Hoesin Hospital Palembang from January to August 2023. Laboratory and imaging examinations were done on each patient. This study was performed to review the demographic characteristic, clinicopathologic data, treatment strategies, and outcomes of HCC patients.</p><p><b><i>Results:</i></b> Ninety-eight HCC patients consist of 72 males (77.4%) and 21 females (22.6%) with an average age of 53.47±11.28 years, from which 18 participants were underweight (18.2%), 17 participants were overweight (18.3%) and 13 participant were obese (32.3%). The majority of causes of HCC are hepatitis B virus (62.4%), hepatitis C virus (5.4%), and other causes (32.3%). Based on the ALBI ratio, Grade 1: 69.9%, Grade 2: 28.0%, and Grade 3: 2.2%. A total of 75.3% participant present with large tumor (≥ 5 cm), 62.4% participant present with multiple nodules, and about 44.1% patients had tumor metastasis or thrombus. Most of the patient with advanced disease got best supportive care (80.6%). Sixteen (17.2%) patients died of their disease, 77 (82.8) patients are alive 3 months after diagnosed.</p><p><b><i>Conclusion:</i></b> Most patients with HCC at Mohammad Hoesin Palembang with advanced disease; however, multiple local and systemic treatments were offered.</p><p><b><i>Keywords:</i></b> Hepatocellular carcinoma</p><p><b>PP-02-204</b></p><p><b>Comparing survival outcomes in less than 2cm and 2-3cm lesions of hepatocellular carcinoma (retrospective study)</b></p><p><b>Vujwal Roy</b>, Anshid Nazar, Jeby Jacob, Jeffey George and KH Ismail Siyad</p><p><i>Aster Medcity, Kochi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> There's limited data from India on the difference between less than 2cm and 2-3cm lesions in Hepatocellular Carcinoma(HCC)</p><p><b><i>Objectives:</i></b> To compare Overall Survival, disease characteristics in less than 2 cm and 2-3cm lesions of HCC</p><p><b><i>Materials and Methods:</i></b> Data of 259 patients were analyzed from 2016 to 2024. 77 patients had less than 3cm diameter. Diagnosis was made based on reports of Triple phase CT(or)MRI of Liver, AFP, or Biopsy. Patients who had undergone transplantation were excluded, and those who had been under follow up for atleast 4 years or died were included.</p><p><b><i>Results:</i></b> Of the 77 patients, 29 patients had less than 2cm lesions, while 48 patients had 2-3cm lesions. The median(IQR) of AFP, INR, Bilirubin were 6.7(3.8,24.9); 1.31(1.18,1.6); 1.3(0.83,2.55) and 10.34(3.79, 1.36(1.22,1.68), 1.2(0.87,2.18) in less than 2cm lesions and 2-3cm lesions respectively.</p><p>Mean±SD of Albumin was 3.31±0.72 and 3.42±0.59 in less than 2cm, and 2-3cm lesions respectively. Mean±SD of Overall Survival in less than 2cm and 2-3cm lesions was 35.25±30.65 months and 29.14±25.08 months respectively.</p><p>AFP, INR, Bilirubin and albumin were not statistically significant in the two groups(p 0.563, 0.433, 0.538, 0.476). Overall survival was non significant in the two groups (0.444).</p><p><b><i>Conclusion:</i></b> While previous studies suggest that survival outcome was better in less than 2cm when compared to 2-3cm lesions.</p><p>The difference in overall survival, AFP, Bilirubin, INR and Albumin was statistically insignificant in this study.</p><p>To conclude, less than 3cm lesions irrespective of whether 2-3cm or less than 2cm have similar overall survival.</p><p><b>PP-02-205</b></p><p><b>Comparison of Alpha-Fetoprotein Levels in Viral and Non-Viral Related Hepatocellular Carcinoma</b></p><p>Mutmainna Said<sup>1</sup>, Muhammad Luthfi Parewangi<sup>2,3</sup>, Susanto H. Kusuma<sup>2,3</sup>, Fardah Akil<sup>2,3</sup>, Nu’man AS Daud<sup>2,3</sup>, Rini Rachmawarni Bachtiar<sup>2,3</sup> and Amelia Rifai<sup>2,3</sup></p><p><sup>1</sup><i>Internal Medicine Specialist Program, Departement of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>3</sup><i>Centre of Gastroenterology-Hepatology HAM Akil Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer worldwide and a major cause of mortality. It is commonly associated with chronic liver diseases, particularly HBV infection, HCV infection, and other etiologies. Approximately 78,200 new cases are diagnosed each year, and the incidence of HCC will increase, estimated peak in the year 2030. Alpha-fetoprotein (AFP) is one of biomarker used for HCC screening and monitoring, although its levels can vary depending on the etiology and severity of the disease. The aim of this study is comparing AFP levels based on the degree of HCC of viral and non-viral etiology</p><p><b><i>Materials and Methods:</i></b> A cross-sectional study was conducted of HCC patients from January to June 2024 at Dr. Wahidin Sudirohusodo Hospital. Patients were categorized based on etiology (viral/non-viral) and The Barcelona Clinic Liver Cancer staging. AFP levels and BCLC stage were statistically analysed using the Kruskal-Wallis test.</p><p><b><i>Results:</i></b> The study included 104 HCC patients, with 86 having viral etiology and 18 non-viral etiology. Patients with viral HCC were more likely to be male, older (≥ 55 years), have larger tumors (>10 cm), more frequent portal vein thrombosis, higher BCLC stages, and elevated AFP levels (>200 ng/ml). AFP levels were significantly higher in the viral group compared to the non-viral group. Additionally, AFP levels increased significantly with the progression of BCLC stages with p-value 0.003.</p><p><b><i>Conclusion:</i></b> Based on the degree of HCC, AFP levels significantly difference in viral related compare to non-viral related cases.</p><p><b>PP-02-206</b></p><p><b>tRF-3a-Pro facilitated the progression of hepatocellular carcinoma by promoting MYC mRNA stability through hnRNP K</b></p><p><b>Jingyi Si</b><sup>1</sup>, Yifan Gao<sup>1</sup>, Changfeng Zhu<sup>1</sup>, Qunyan Yao<sup>1,2,3</sup> and Xizhong Shen<sup>1</sup></p><p><sup>1</sup><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Xiamen, China;</i> <sup>3</sup><i>Shanghai Geriatric Medical Center, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> In this study we aimed to explore the molecular mechanism of tRF-3a-Pro regulating the biological function of HCC cells through RNA binding proteins.</p><p><b><i>Methods:</i></b> The effect of tRF-3a-Pro on biological function of HCC cells was observed by CCK-8, EdU, clone formation and cell cycle assays. RNA pull-down and protein mass spectrometry assay were used to screen for proteins that bind to tRF-3a-Pro. FISH and immunofluorescence experiments were used to determine the intracellular localization of tRF-3a-Pro and hnRNP K. We obtained downstream genes through bioinformatics analysis. The half-life of mRNA of downstream gene MYC was evaluated through ACTD treatment. RIP experiments were used to evaluate the effect of tRF-3a-Pro on the binding ability of hnRNP K to MYC mRNA.</p><p><b><i>Results:</i></b> tRF-3a Pro could promote the proliferation of HCC cells. We screened hnRNP K as a tRF-3a-Pro binding protein and validated the interaction between tRF-3a-Pro and hnRNP K within the nucleus. Knocking down hnRNP K could inhibit cell proliferation and coloy formation ability as well as tRF-3a-Pro. MYC was identified as downstream gene co-regulated by tRF-3a-Pro and hnRNP K. Subsequent studies revealed that tRF-3a-Pro and hnRNP K decreased the expression of MYC by regulating the stability of MYC mRNA. Finally, RIP experiments results suggested that tRF-3a-Pro reduced the half-life of MYC mRNA by affecting the binding ability of hnRNP K to MYC mRNA.</p><p><b><i>Conclusion:</i></b> tRF-3a-Pro enhances the stability of MYC mRNA by promoting its combination to hnRNP K, thereby regulating the proliferation of HCC cells.</p><p><b>PP-02-207</b></p><p><b>Expression and diagnostic value of tRNA derived fragment tRF-3a-Pro in HCC</b></p><p><b>Qunyan Yao</b><sup>1,2,3</sup>, Jingyi Si<sup>1</sup>, Yifan Gao<sup>1</sup>, Xizhong Shen<sup>1</sup> and Changfeng Zhu<sup>1</sup></p><p><sup>1</sup><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Xiamen, China;</i> <sup>3</sup><i>Shanghai Geriatric Medical Center, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aims to identify the expression characteristics of tsRNAs in HCC tissue, screen and verify the upregulated tsRNAs in tumor tissue and serum of HCC patients, and preliminarily evaluate the diagnostic value of serum tsRNA for HCC.</p><p><b><i>Methods:</i></b> We performed tsRNA sequencing in 5 pairs of HCC tumor and adjacent tissues, and screened for differentially expressed tsRNAs by p-value and fold changes. The top 4 upregulated tsRNAs in the sequencing results were validated in HCC tumor/adjacent tissues and serum of HCC patients/control population through quantitative real-time polymerase chain reaction (qRT-PCR). A diagnostic model of alpha fetoprotein (AFP) and tRF-3a-Pro was established for HCC by logistic regression, with liver cirrhosis patients and healthy control individuals as control groups.</p><p><b><i>Results:</i></b> The main differential expressioned tsRNA in HCC tissues were tRF-3a and tiRNA-5, with the main upregulated subtype being tRF-3a. The upregulation of tRF-3a-Ala-AGC, tRF-3a-Tyr-GTA, tRF-3a-Pro-CGG, and tRF-3aAla-CGC in 28 pairs of tumor/adjacent tissues was confirmed by qRT-PCR. Only the amount of serum tRF-3a-Pro in HCC patients was significantly increased compared with the control group (AUC = 0.8393, cutoff value = 1.4197). The combination of AFP with tRF-3a-Pro improved the AUC to 0.8884 in the training population with combining predictors = -1.5897 + 0.433 × tRF-3a-Pro + 0.0821 × AFP (cutoff value = -0.2441). And validation was conducted in the validation set population (AUC = 0.8805).</p><p><b><i>Conclusion:</i></b> tRF-3a-Pro is upregulated in tumor tissue and serum of HCC patients, and performs well in the diagnosis of HCC, especially when combined with AFP.</p><p><b>PP-02-208</b></p><p><b>HCC picked up through screening showed better survival outcomes</b></p><p><b>Benedix Sim</b></p><p><i>National University Hospital, Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>We conducted a multicentre screening cohort study evaluating survival outcomes between screening and non-screening populations.</p><p><b><i>Methods:</i></b> In this multicentre retrospective cohort study, HCC patients diagnosed through ultrasound screening, incidentally, or symptomatically were enrolled from 6 tertiary healthcare institutions. Survival outcomes were evaluated between screening and non-screening populations, with secondary outcomes stratifying the populations based on HCC aetiology, cirrhosis status, age, sex, ethnicity, and BCLC staging.</p><p><b><i>Results:</i></b> In total, 1185 patients were included in the analysis (178 MASH HCC, 168 alcohol-associated HCC, 422 HBV HCC, 395 HCV HCC, and 128 patients with unknown etiology), 975 patients were diagnosed through screening while 210 patients were diagnosed incidentally or symptomatically. Survival analysis showed statistically significant differences in restricted mean survival time (RMST) based on screening status (p < 0.001), with an RMST of 8.369 years (95% CI: 7.886 - 8.851) amongst screening patients and 4.401 years (95% CI: 3.479 - 5.323) amongst non-screening patients. By aetiology, HBV HCC had the largest difference between screening and non-screening cohorts (RMST ratio: 1.966, 95% CI: 1.496 - 2.583, p < 0.001), followed by ALD HCC (RMST ratio: 1.765, 95% CI: 1.262 - 2.469, p = 0.001) and HCV HCC (RMST ratio: 1.687, 95% CI: 1.200 - 2.374, p = 0.003). Multivariate analysis showed significant differences between screening and non-screening populations after adjusting for cirrhosis, gender, age, and ethnicity.</p><p>In conclusion, HCC surveillance is associated with improved early detection and survival in patients.</p><p><b>PP-02-209</b></p><p><b>Clinical outcomes and prognostic factors of transarterial chemoembolization in patients with solitary large hepatocellular carcinoma</b></p><p>Maneerat Chayanupatkul, Sorrawit Mukdavannakorn, Thatchai Srimuninnimit, Nichanut Rattanavit, <b>Chanchanok Suriyaammaranon</b> and Nutcha Pinjaroen</p><p><i>Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There is a scarcity of data regarding the outcomes of Transarterial chemoembolization (TACE) in treatment-naïve hepatocellular carcinoma (HCC) patients with tumors larger than 10 cm. This study aimed to evaluate the efficacy of TACE and identify clinical factors that predict outcomes in this patient subgroup.</p><p><b><i>Materials and Methods:</i></b> We retrospectively analyzed treatment-naïve patients with a solitary large HCC (defined by a tumor size of at least 10 cm) and no extrahepatic metastasis. All patients received TACE as their initial treatment. Overall survival (OS) was calculated, and data on demographics, clinical specifics, tumor characteristics, and laboratory results were collected.</p><p><b><i>Results:</i></b> 120 patients were included. The median age was 61.5 years (interquartile range (IQR): 7.8 years), with 104 patients (86.7%) being male. The median tumor diameter was 14 cm (IQR: 2.2 cm), and vascular invasion was present in 57 patients (47.5%). Median OS was 45 months (95% CI: 27 – 79 months), with OS rates of 69.2% at 6 months, 46.7% at 1 year, and 27.5% at 2 years. A larger tumor diameter was associated with increased mortality risk (OR 1.29, p = 0.04). Tumor rupture, portal vein invasion, underlying cirrhosis, and alpha-fetoprotein levels at diagnosis did not predict worse outcomes. Only 14 patients (11.7%) achieved complete radiologic tumor response with TACE as a monotherapy.</p><p><b><i>Conclusion:</i></b> Treatment-naïve patients with a solitary large HCC larger, who underwent TACE as the initial therapy, showed reasonable overall survival rates. Larger tumor size was the only significant predictor associated with reduced survival outcomes.</p><p><b>PP-02-210</b></p><p><b>Therapeutic Effectiveness of Early Additional cTACE after DEB-TACE for Hepatocellular Carcinoma</b></p><p><b>Kei Takuma</b>, Kazutoshi Fuke, Joji Tani, Asahiro Morishita and Hideki Kobara</p><p><i>Kagawa university, Ikenobe, Miki-town, Kita-gun, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Purpose:</i></b> We have previously reported that early additional cTACE after drug-eluting beads TACE (DEB-TACE) significantly increased the CR rate compared to patients treated with DEB-TACE alone. In the present study, we investigated how early additional cTACE should be performed after DEB-TACE in patients with hepatocellular carcinoma (HCC) at a multicenter setting.</p><p><b><i>Methods:</i></b> Twenty-seven patients with unresectable hepatocellular carcinoma (HCC) with a maximum tumor diameter of 30 mm or greater, Child-Pugh classification A-B, and ECOG-PS 0-2, who were treated at four institutions including our hospital between July 2014 and November 2022, were included in this study. The subjects undergone DEB-TACE first and cTACE as the second treatment. The overall response rate (ORR) and disease control rate (DCR) were evaluated retrospectively by mRECIST.</p><p><b><i>Results:</i></b> The overall evaluation by initial DEB-TACE was CR/PR/SD/PD=0/27/0/0, and the overall response rate and disease control rate were 100%. The overall response rate was 75.0% and disease control rate was 91.7% in the group that received the next cTACE treatment earlier than DEB-TACE (CR/PR/SD/PD=1/8/2/1), whereas the overall response rate and disease control rate in the group that received the next cTACE treatment more than 3 months after conventional DEB-TACE were The overall response rate, CR/PR/SD/PD=2/3/6/4, overall response rate, and disease control rate were 33.3% and 73.3%, respectively, indicating a significant difference in overall response rate between the two groups (p=0.031).</p><p><b><i>Conclusion:</i></b> In unresectable hepatocellular carcinoma, early additional cTACE after DEB-TACE can bring better therapeutic effect.</p><p><b>PP-02-212</b></p><p><b>The Impact of Metabolic Abnormalities on Prognosis of Lean Patients with Hepatocellular Carcinoma Receiving Surgery</b></p><p><b>Chih Hsuan Wang</b><sup>1,2,4</sup>, Kung Hung Lin<sup>3</sup> and Jee Fu Huang<sup>4,5,6,7</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan;</i> <sup>2</sup><i>Division of Gastroenterology and Hepatology, Tri-Service General Hospital, Taipei, Taiwan;</i> <sup>3</sup><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;</i> <sup>4</sup><i>Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;</i> <sup>5</sup><i>Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;</i> <sup>6</sup><i>Hepatitis Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;</i> <sup>7</sup><i>Center of Excellence for Metabolic Associated Fatty Liver Disease, National Sun Yat-sen University, Kaohsiung, Taiwan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Obesity is associated with an increased risk of HCC. However, whether a higher body mass index (BMI) confers a survival benefit in HCC patients remains unclear. We conducted this study to investigate survival in HCC patients after hepatic resection according to BMI and analyzed the impact of metabolic abnormalities between lean and non-lean HCC patients.</p><p><b><i>Materials and methods:</i></b> This retrospective cohort study recruited pathologically-proven HCC patients receiving surgical resection between 2013-2021 in a medical center in southern Taiwan. We categorized the patients into four groups: BMI ≥23(also called non-lean) with ≥ 1 cardiometabolic risk factor (CMRF) group (A), BMI ≥23 without CMRF group (B), normal BMI(also called lean) with ≥ 1 CMRF group (C), and normal BMI without CMRF group (D). We also analyzed the etiologies, fibrosis stage, liver-related events, cardiovascular events and 5-year survival rate of the HCC.</p><p><b><i>Results:</i></b> A total of 1,208 HCC patients were recruited. Patients of group C had a significantly higher proportion of HCV infection (38.1%). Group C had a significantly lower 5-year survival rate than patients of group A (p = 0.007) and group B (p < 0.001). In the subgroup analysis, group C had the lowest 5-year survival rate in both the HCV subgroup and the non-B-non-C etiologies.</p><p><b><i>Conclusion:</i></b> The lean individuals with metabolic abnormalities have a lower five-year survival rate. This phenomenon is more pronounced in patients with HCV or non-B-non-C etiologies. Therefore, rigorous follow-up with interventions for metabolic abnormalities are recommended for lean patients with HCC.</p><p><b>PP-02-213</b></p><p><b>DCAF13 promotes the progression of hepatocellular carcinoma through the AKT/PI3K/mTOR pathway</b></p><p><b>Hongchen Zhang</b><sup>1,2,3</sup>, Dongchao Xu<sup>1,2,3</sup>, Yuanhui Li<sup>1,2,3</sup> and Xiaofeng Zhang<sup>1,2,3</sup></p><p><sup>1</sup><i>Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University, Hangzhou, China;</i> <sup>2</sup><i>Key Laboratory of Integrated Traditional Chinese and Western Medicine for Biliary and Pancreatic Diseases of Zhejiang Province, Hangzhou, China;</i> <sup>3</sup><i>Hangzhou Institute of Digestive Disease, Hangzhou, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The DCAF13 gene, located on chromosome Xq24, encodes a protein that functions as a substrate recognition module within the CUL4-DDB1 E3 ubiquitin ligase complex. Its biological role in hepatocellular carcinoma (HCC) remains unexplored. This study aims to examine the expression of DCAF13 in HCC cell lines and its influence on the biological behavior of these cells.</p><p><b><i>Materials and Methods:</i></b> The Cancer Genome Atlas (TCGA) database was utilized for bioinformatic analysis. HCC cell lines with DCAF13 overexpression and silencing were established using lentiviral constructs. Western blotting was performed to evaluate DCAF13 expression. Functional assays included the cell counting kit-8 (CCK-8) assay, EdU assay, and flow cytometry for apoptosis analysis.</p><p><b><i>Results:</i></b> Bioinformatic analysis of the TCGA database indicated elevated DCAF13 expression in HCC. Overexpression and silencing experiments revealed that DCAF13 significantly influences cell proliferation and apoptosis. Functional assays demonstrated that DCAF13 promotes HCC progression by activating the AKT/PI3K/mTOR pathway, as supported by gene set enrichment analysis (GSEA).</p><p><b><i>Conclusion:</i></b> Our study highlights the critical role of DCAF13 in HCC progression. DCAF13 is highly expressed in HCC and regulates cell proliferation and apoptosis. Targeting DCAF13 disrupts cell proliferation and enhances apoptosis, thereby inhibiting the growth of HCC cells.</p><p><b>PP-02-214</b></p><p><b>A Successful SVR After Three Regimens Of Hepatitis C</b></p><p><b>Alida Avisiena</b>, Poernomo Boedi Setiawan, Ummi Maimunah, Budi Widodo and Titong Sugihartono</p><p><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Airlangga University – Dr Soetomo General Academic Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Since its discovery in 1989, Hepatitis C Virus (HCV) has become cause of chronic liver disease. The evolution of HCV therapy in Indonesia follows the discovery of anti-virals in the world. Monitoring side effects and kidney function is important in the management of HCV and also how to manage HCV patients with Chronic Kidney Disease.</p><p><b><i>Case Description:</i></b> A 77 years old man with Hepatitis C and Chronic Kidney Disease (eGFR 26 ml/minute/1.73 m2) was treated at Dr. Soetomo Hospital Surabaya Indonesia. In 2014, patient received dual therapy (Peg-IFN/RBV) and achieved Early Virological Response. Serious side effects (low hemoglobin level and severe thrombocytopenia appeared at week-14. Therapy was stopped at week-16. In 2015 HCV RNA was redetected. Eight years later patient received Direct Acting Antiviral (DAA) Elbasvir/Grazoprevir for 12 weeks and only achieved Delayed Virological Response. There was an increase of eGFR. Regimen was changed to Sofosbuvir alternate day (once daily) and Daclatasvir once daily. Estimeted GFR worsened after 6 weeks, although Sofosbuvir was reduced 2 times a week, then therapy was discontinued. Totally, DAA was given in 18 weeks. After stopping, HCV RNA was undetected and remained undetected after 12 weeks (SVR).</p><p><b><i>Discussion:</i></b> Serious side effects such as anemia and thrombocytopenia often found when using Peg-IFN/RBV as a consequence, therapy was decided to stop and SVR could not be achieved. When using the Elbasvir/Grazoprevier regimen, no dose adjustments for eGFR < 30 ml/minute/1.73 m2. In this case there was an increase in eGFR. However, SVR was achieved after SOF-DCV.</p><p><b>PP-02-215</b></p><p><b>Factors associated with hepatitis B surface antigen sero-clearance in hemodialysis patients</b></p><p><b>Kiran Bajaj</b> and Taha Raja Yaseen</p><p><i>Sindh institute of urology and transplantation, Karachi, Pakistan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hemodialysis is a route to many infections with hepatitis B one of the most commonly encountered pathogen. The data regarding the factors predictive of hepatitis B surface antigen (HBsAg) clearance in hemodialysis patients is scarce. our aim was to determine the rate of hepatitis B surface antigen clearance in hemodialysis patients and also to identify the factors predictive of HBsAg clearance in this population.</p><p><b><i>Methods:</i></b> It was prospective observational study which was conducted at the department of Hepatogastroenterology, Sindh Institute of Urology and Transplantation. All the patients with HBsAg reactive chronic hepatitis B undergoing hemodialysis from January 2019 to December 2020 were included in the study. Univariate followed by multivariate logistic regression analysis was performed to identify independent predictors of HBsAg loss in hemodialysis patients.</p><p><b><i>Results:</i></b> A total of 213 patients with chronic HBV undergoing hemodialysis patients were included in the study. Out of them, 163(76.5%) were males. At baseline, HBsAg levels > 1000 IU/ml were noted in 159(74%) patients, HBV DNA > 5000 were observed in 109(51.1%) and HbeAg positive disease was noted in 52(24.4).Most of the patients i.e.152(71.3%) underwent single session of hemodialysis per week. HBsAg loss was noted in 26(12%) patients. On univariate analysis, HBsAg levels < 1000IU/ml, HBV DNA levels <5000IU/ml, HBeAg negative disease and multiple sessions of hemodialysis was associated with increased HBsAg loss while on multivariate analysis, HBV DNA levels of less than 5000 IU/ml at baseline and multiple sessions of hemodialysis were independent predictors of HBsAg loss in hemodialysis patients.</p><p><b>PP-02-216</b></p><p><b>Correlation Between Interleukin-10 Serum Levels and Liver Fibrosis Severity in Treated Chronic Hepatitis B Patients</b></p><p><b>Diah Rizki Rahma Dini</b><sup>1</sup>, Hendy Wirantara<sup>1</sup>, Ulfa Kholili<sup>2</sup> and Ummi Maimunah<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Airlangga University Hospital, Surabaya, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aimed to investigate the relationship between serum IL-10 levels and liver fibrosis severity in patients with chronic hepatitis B.</p><p><b><i>Method:</i></b> The population of this study is chronic hepatitis B patients who have received antiviral treatment for at least one year. We used ELISA to determine the serum IL-10 levels and Fibroscan to assess the degree of liver fibrosis, which was then categorized as mild, significant, or advanced. The Spearman correlation test and linear regression model were used to analyze the data, with the results presented as a p-value and correlation coefficient of p<0.05 considered statistically significant.</p><p><b><i>Result:</i></b> With an average age of 42,69 years, the 80 participants in this study were primarily male (62,5%). The serum level of IL-10 was 10.8 (1.7-176.1) pg/ml at the median. Liver stiffness median is 6.45 ranged from 2.6-35.3 kPa. Serum levels of IL-10 and the severity of their liver fibrosis showed a significant moderate positive correlation (p<0.001; r=0,408). Linear regression model showed a 2.7 pg/mL increment of IL-10 was associated with increase 1 kPa value in liver stiffness measurement (95%CI: 1.65 - 3.79; r2 = 0.2056).</p><p><b><i>Conclusion:</i></b> The results suggest that elevated serum IL-10 levels are associated with more severe liver fibrosis with in chronic hepatitis B patients treated with antiviral therapy.</p><p><b>PP-02-217</b></p><p><b>Chronic hepatitis b and covid-19 coinfection among admitted patients in a hospital in the philippines</b></p><p><b>Angeli Eleanor Facun</b>, Bernard Demot and Marie Ellaine Velasquez</p><p><i>Baguio General Hospital and Medical Center, Baguio City, Philippines</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This paper aims to present the prevalence and outcomes of adult patients with COVID-19 and Chronic Hepatitis B coinfection at a tertiary hospital in the Philippines.</p><p><b><i>Materials and Methods:</i></b> A cross-sectional descriptive study was utilized in this study.</p><p><b><i>Results:</i></b> Among the 5255 Adult COVID-19 patients admitted, only 519 (10%) patients were tested for hepatitis B. Excluding those with incomplete hepatitis profile, the sample population is 311 (60%) which was classified as follows: HBeAg Positive Chronic Hepatitis B (n=0, 0%), Chronic hepatitis B with low infectivity (n=19, 6.1%), occult hepatitis B infection (n=12, 3.9%), recovered from Chronic Hepatitis B (n=60, 19.3%), vaccinated from hepatitis B (n=84, 27%) and susceptible to Chronic Hepatitis B (n=136, 43.7%). There were a total of 10 mild COVID 19 cases, 30 moderate cases, 168 severe cases and 103 critical cases. In terms of mortality rate, Chronic hepatitis B with low infectivity had 5.3%, occult hepatitis B infection had 33%, recovered from Chronic Hepatitis B had 30%, vaccinated from hepatitis B had 27.4% and susceptible to Chronic Hepatitis B had 27.2%. The average length of hospital stay was similar and lastly, in terms of complications, acute respiratory failure had the highest percentage of complication across all subgroups.</p><p><b><i>Conclusion:</i></b> Chronic Hepatitis B is still identified as a disease with high burden in the Philippines. This study reflects that there really is a need to concentrate and direct more effort on these subset of vulnerable populations especially during the time of the pandemic.</p><p><b>PP-02-218</b></p><p><b>Reliability Of AFP Level Compared With APRI In Liver Fibrosis Prediction In Chronic Hepatitis B</b></p><p><b>Ety Febrianti</b><sup>1</sup>, Richard Lumbantobing<sup>1</sup>, Febry Rahmayani<sup>1</sup>, Suyata Suyata<sup>2</sup> and Legiran Legiran<sup>3</sup></p><p><sup>1</sup><i>Internal Medicine Moh. Hoesin Hospital/Sriwijaya University, Palembang, Indonesia;</i> <sup>2</sup><i>Gastroenterohepatology Division, Internal Medicine Moh. Hoesin Hospital/Sriwijaya University, Palembang, Indonesia;</i> <sup>3</sup><i>Biomedical Division, Faculty of Medicine Sriwijaya University, Palembang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Chronic hepatitis B (CHB) is associated with an increased risk of liver fibrosis. Non-invasive methods such as aspartate aminotransferase to platelet ratio index (APRI) require simple laboratory values that can predict liver fibrosis in chronic hepatitis B patients. Alpha-fetoprotein (AFP) can be used indirectly as an index to indicate the stage of fibrosis in chronic hepatitis B.</p><p><b><i>Objective:</i></b> This study aims to assess reliability of AFP in liver fibrosis prediction based on APRI in CHB patients at the vertical hospital Dr. Mohammad Hoesin Palembang.</p><p><b><i>Methods:</i></b> This is a cross-sectional study, on 40 naive Hepatitis B virus infection patients who follow the laboratory examination at the Internal Medicine Clinic at the vertical hospital Dr. Mohammad Hoesin from January 2023 to August 2023.</p><p><b><i>Result:</i></b> This research involved 40 subjects, the majority of chronic hepatitis B patients were women (57.5%) and aged 18-60 (92.5%). The results of the analysis of the AFP consistency test against APRI show a p value <0.001 and a Kappa coefficient strength of agreement value of 0.391 or fair.</p><p><b><i>Conclusion:</i></b> Examination of AFP level has fair reliability and consistency with APRI in predicting liver fibrosis in chronic hepatitis B patients.</p><p><b><i>Keywords:</i></b> Chronic hepatitis B, Alpha fetoprotein, Aspartate Aminotransferase to Platelet Ratio Index, Liver fibrosis.</p><p><b>PP-02-219</b></p><p><b>Long-term outcomes of HCC recurrence and occurrence after hepatitis C virus eradication by DAA treatment</b></p><p><b>Kazutoshi Fuke</b>, Joji Tani, Kei Takuma, Asashiro Morishita and Hideki Kobara</p><p><i>Faculty Of Medicine, Kagawa University, Miki-cho Kagawa, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> DAA treatment (DAAt) have increased the cure rate for HCV patients, but the rates of HCC occurrence and long-term outcomes in those with HCC recurrence post-DAAt are unclear.</p><p><b><i>Methods:</i></b> The study first aimed to identify predictors of HCC occurrence post-DAAt. Among 1218 HCV-infected patients, 1088 who achieved SVR and had no history of HCC treatment were included between September 2014 and November 2018.</p><p><b><i>Results:</i></b> HCC incidence was 0.61%, 1.88% and 3.71% at 6, 12 and 24 months post-DAAt, respectively. Multivariate analysis revealed age (HR 1.0729, P=0.0044) and AFP levels post-DAAt (HR 1.0486, P=0.0486) as independent predictors of HCC. A scoring system (0-2 points) was developed using these factors to predict HCC occurrence. HCC incidence at 2 years was 0.3% for 0 points, 6.27% for 1 point, and 18.37% for 2 points. The second aim was to investigate recurrence rates, factors, and prognosis in 130 patients treated with DAAt after HCC treatment. HCC recurrence rates were 32.5%, 46.3%, and 59.4% at 12, 24 and 36 months, respectively. Multivariate analysis showed palliative treatment prior to DAAt (HR 3.974, 95% CI 1.924–8.207, P=0.0006) and AFP at SVR12 (HR 1.048, 95% CI 1.016–1.077, P=0.0046) were independent factors for HCC recurrence. Overall survival rates at 12, 24, and 36 months were 97.6%, 94.0%, and 89.8%, respectively.</p><p><b><i>Conclusions:</i></b> Age and AFP level post-DAAt are independent predictors of HCC occurrence. This scoring system can help predict HCC risk in HCV-free patients post-DAAt. Despite recurrence rates, the prognosis improved, indicating DAAt should be considered for HCV patients.</p><p><b>PP-02-220</b></p><p><b>Interleukin-6 as a Biomarker for Assessing Liver Fibrosis Severity in Patients with Chronic Hepatitis B</b></p><p><b>Amal Arifi Hidayat</b><sup>1</sup>, Rheza Rahmadika Putra<sup>1</sup>, Ulfa Kholili<sup>2</sup> and Ummi Maimunah<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aimed to examine the relationship between IL-6 serum levels and the severity of liver fibrosis in patients with chronic hepatitis B.</p><p><b><i>Materials and Methods:</i></b> An observational cross-sectional analytic study involving chronic hepatitis B patients who received antiviral therapy for at least 1 year. All subjects were examined for IL-6 serum levels and liver fibrosis severity. IL-6 serum levels was measured by ELISA method. Liver fibrosis severity was determined using transient elastography and categorized as follow: mild fibrosis (F0-F1), significant fibrosis (F2-F3) and advance fibrosis (F4). Data were analyzed using Kruskal-Wallis test.</p><p><b><i>Results:</i></b> This study involved 78 subjects predominantly males (66.7%) with the average of age 43.05 + 10.77 years old. The median of IL-6 serum level was 6.75 (0.2-96.5) pg/ml. The median of liver stiffness was 6.35 + 8 kPa, with the proportion of mild fibrosis (16/78, 20.5%), significant fibrosis (21/78, 26.9%) and advance fibrosis (41/78, 52.5%). There was a significant association between IL-6 serum levels with liver fibrosis severity for mild, significant and advance fibrosis (4.1 pg/m vs 4.65 pg/m vs 11 pg/m, respectively; p < 0.001).</p><p><b><i>Conclusion:</i></b> The findings of this study suggest that elevated IL-6 serum levels was associated with more severe liver fibrosis in patients with chronic hepatitis B.</p><p><b>PP-02-221</b></p><p><b>Relationship between hepatic fibrosis and several basic laboratory parameters in non-chirrotic chronic hepatitis C subjects</b></p><p><b>Boy Hutaperi</b><sup>1</sup> and Putut Bayupurnama<sup>2</sup></p><p><sup>1</sup><i>Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia;</i> <sup>2</sup><i>Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hepatic fibrosis and its sequence of cirrhosis remain important problem in managing chronic hepatitis C. Unfortunately, sophisticated hepatic fibrosis assessment methods are not widely available. Requirement of basic laboratory test take place into this gap.</p><p><b><i>Objective:</i></b> Evaluate correlation between hepatic fibrosis state and several basic laboratory parameters in non-chirrotic chronic hepatitis C.</p><p><b><i>Methods:</i></b> Retrospective study was conducted at Sardjito hospital, enrolled 72 non-cirrhotic hepatitis C from January 2022 to February 2024. Basic laboratory measures such hemoglobin, platelet, ALT, AST, albumin and HCV-RNA level were collected. Mild and significant fibrosis group was defined by liver stiffness measurement (Fibroscan®).</p><p><b><i>Results:</i></b> Among 72 non-cirrhotic hepatitis C subjects, mean age was 47.8 ± 13.2 years and 54 (74%) were men. Significant fibrosis was found in 37 (50.7%) subjects. Mann-Whitney test did not show any significant differences in terms of hemoglobin, ALT level and HCV-RNA between two group. Albumin level was higher significantly in mild fibrosis group (p = 0.032), meanwhile age and AST level were lower significantly in mild fibrosis group (p = 0.002 and p = 0.015 respectively).</p><p>Spearman correlation test revealed that age and AST level correlate positively with fibrosis state (r = 0.3; p = 0,014 and r = 0.4; p = 0.002, respectively). On contrary, hemoglobin, platelet, albumin, ALT level and HCV-RNA did not correlate positively to fibrosis.</p><p><b><i>Conclusion:</i></b> Age and AST level solely had significantly positive correlation to fibrosis stage in non-chirrotic chronic hepatitis C</p><p><b>PP-02-222</b></p><p><b>Treatment efficacy and safety of sofosbuvir and velpastavir based treatement in Korea: Multi-institutional prospective study</b></p><p><b>Sanghyuk Jung</b> and Ga Ram You</p><p><i>Chonnam National University Hospital, Gwangju, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Direct-acting antivirals (DAAs) have revolutionized Hepatitis C virus (HCV) treatment, significantly improving efficacy. The regimen of sofosbuvir, velpatasvir, and voxilaprevir has shown high efficacy across all Hepatitis C virus genotypes. This study investigates their real-world efficacy and safety profile in South Korea.</p><p><b><i>Methods:</i></b> From November 2022 to January 2024, patients with HCV undergoing SOF/VEL-based treatment at six hospitals were enrolled. Patients from chronic hepatitis to decompensated liver cirrhosis, received sofosbuvir and velpatasvir (SOF/VEL) for 12 weeks. Patients who failed to achieve sustained virological response (SVR) with previous DAA treatments, from chronic hepatitis to compensated liver cirrhosis, received sofosbuvir, velpatasvir, and voxilaprevir (SOF/VEL/VOX) for 12 weeks. Exclusions included patients with hepatocellular carcinoma within six months.</p><p><b><i>Results:</i></b> Among 101 patients treated with SOF/VEL, the mean age was 64.71 years, with 40.9% male. Genotype distribution was 40.6% genotype 1b and 59.4% genotype 2. The mean baseline HCV RNA level was 3,088,097 IU/mL. Underlying compensated liver cirrhosis was present in 21.8% and decompensated in 5.0%. SVR12 was achieved in 86.1% (87 patients), while 13.9% (14 patients) were lost to follow-up.</p><p>Among 17 patients on SOF/VEL/VOX, the mean age was 61.84 years, with 29.4% male. Fourteen patients had previously failed DAA treatment, one had achieved SVR, one had self-stopped DAA, and one did not check SVR. Four had compensated liver cirrhosis. SVR12 was achieved in all 16 assessable patients. No serious adverse events (≥grade 3) were reported.</p><p><b><i>Conclusion:</i></b> Sofosbuvir and velpatasvir-based treatments demonstrated excellent SVR12 rates and a favorable safety profile.</p><p><b>PP-02-223</b></p><p><b>The effect of tenofovir alafenamide on alanine aminotransferase levels in chronic hepatitis B patients</b></p><p><b>Lilian Yan Liang</b><sup>1</sup>, Jimmy Che-To Lai<sup>1,2</sup>, Terry Cheuk-Fung Yip<sup>1</sup>, Vincent Wai-Sun Wong<sup>1</sup> and Grace Lai-Hung Wong<sup>1</sup></p><p><sup>1</sup><i>Medical Data Analytic Centre (MDAC), Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, SAR, China;</i> <sup>2</sup><i>Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong, SAR, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Tenofovir alafenamide (TAF) is known to improve alanine aminotransferase (ALT) levels and we aimed to evaluate ALT level changes in TAF-treated chronic hepatitis B (CHB) patients with or without exposure to other antiviral drugs.</p><p><b><i>Materials and methods:</i></b> This is a retrospective cohort study including TAF-treated CHB patients with available ALT levels. Patients exposed to other nucleos(t)ide analogues (NAs) after starting TAF were combined with those treated only with TAF, with follow-up censored at the start of the other NAs. Those who received other NAs before TAF were classified as another group. Baseline was defined as the start date of TAF treatment.</p><p><b><i>Results:</i></b> This study included 4,977 TAF-treated CHB patients, with a mean age of 61 years and 65.5% males. The corresponding ALT levels (U/L) at baseline, month 3, 6, 9, 12 were 28.8 [interquartile range: 21.0–50.0], 26.3 [19.0–36.0], 25.0 [18.0–36.0], 24.8 [17.9–35.0], 25.0 [19.0–35.0] for TAF-treated only patients and 26.0 [19.7–38.0], 24.0 [17.0–35.0], 23.0 [17.3–32.0], 23.7 [17.0–33.0], 23.0 [17.0–31.4] for TAF-treated patients exposed to other NAs (Figure). The percentages of patients with normal ALT at baseline were 77.5% and 68.6% (P < 0.05) for TAF-treated patients with and without exposure to other NAs and increased to 86.5% and 83.3% (P = 0.189) at 12 months, respectively.</p><p><b><i>Conclusion:</i></b> TAF treatment reduced the ALT levels regardless of exposure to other NAs and increase the incidence of ALT normalization to comparable levels at 12 months between those with and without other NAs.</p><p><b>PP-02-224</b></p><p><b>Comparison One Year Treatment with Tenofovir and Adefovir in Chronic Hepatitis B Patients in Indonesia</b></p><p><b>Ummi Maimunah</b><sup>1</sup>, Muhammad Miftahussurur<sup>1</sup>, Firda Iragama Wessless<sup>1</sup>, Juniastuti Juniastuti<sup>2</sup> and Yoshio Yamaoka<sup>3</sup></p><p><sup>1</sup><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine - Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>2</sup><i>Department of Microbiology, Faculty of Medicine/Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>3</sup><i>Department of Enviromental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Evaluation of the Tenofovir and Adefovir as chronic hepatitis B treatment is rare in Indonesia. We evaluated Tenofovir and Adefovir therapy in chronic hepatitis B patients after one-year treatment.</p><p><b><i>Patients and methods:</i></b> This study is an observational analytic study that included 74 chronic hepatitis B adult patients was determined based on the biochemical, improvement of fibrosis, and virological status.</p><p><b><i>Results:</i></b> Most of the patients were HBeAg positive (43/74, 58.10%) and received treatment at 31–50 aged years (42/74, 56.75%). Significant differences in alanine aminotransferase (ALT) improvement between tenofovir-treated and adefovir-treated patients in HBeAg-positive patients (18/26, 69.23% vs. 5/17, 29.41%; P<0.05). In addition, based on the improvement of fibrosis status, there was a significant difference between tenofovir and adefovir treated patients in TE value (15/26, 57.69% vs. 8/17, 47.05%; P<0.05) and degree of fibrosis (10/26, 38.46% vs. 8/17, 47.05%; P<0.05) both in the HBeAg-positive and negative groups. Furthermore, based on virological status among patients receiving tenofovir, there was a significant difference in median HBV DNA between HBeAg-positive and HBeAg-negative groups before and after one year of treatment (1.7×106IU/ml vs.7.1×107IU/ml; P<0.05) and (6.5×105IU/ml vs. 2.8×106IU/ml; P<0.05, respectively). A significant difference in median value reduction was also found between tenofovir and adefovir patients in the HBeAg-positive group (1.08×108IU/ml vs. 3.9×107IU/ml; P<0.05).</p><p><b><i>Conclusion:</i></b> After one year of treatment, tenofovir was advanced to reducing the virus and improving ALT and fibrosis compared to adefovir.</p><p><b>PP-02-225</b></p><p><b>Elevated Serum TNF-α Levels Correlate with Increased Liver Fibrosis in Treated Chronic Hepatitis B Patients</b></p><p><b>Ummi Maimunah</b><sup>1</sup>, Dio Brimantyo<sup>2</sup> and Ulfa Kholili<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aiming to analyze the correlation between serum TNF-α level and degree of liver fibrosis in chronic hepatitis B patients in the Gastroenterohepatology Outpatient Installation at Dr. Soetomo Hospital Surabaya.</p><p><b><i>Methods:</i></b> This was a cross-sectional study including 82 treated chronic hepatitis B subjects. TNF-α serum levels were measured using specific monoclonal antibodies via a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kit. Transient elastography result was classified according to METAVIR score, F0, F1, F2, F3, and F4. Data was analyzed by the Spearman correlation test and liner with a p<0.05 was considered statistically significant.</p><p><b><i>Results:</i></b> The study included 82 predominantly male participants, with an average age of 42.22 years. The median serum TNF-α level was 8.70 pg/mL, ranging from 0.40 pg/mL to 312.40 pg/mL. The median liver stiffness, a measure of fibrosis, was 6.35 kPa, with values ranging from 2.6 kPa to 27.6 kPa. There was significant association between TNF-α value with the METAVIR score (p < 0.01). Regression model showed increased TNF-α value of 19.9 in each increase fibrosis stage (95%CI = 10.32 - 29.64, p < 0.001)</p><p><b><i>Conclusion:</i></b> The findings of this study suggest that higher serum TNF-α levels are associated with increased liver fibrosis in chronic hepatitis B patients</p><p><b>PP-02-226</b></p><p><b>SCFA and Butyrate Level Relationship with APRI and FIB-4 Score in Hepatitis B or C</b></p><p>Syifa Mustika<sup>1</sup>, Mochamad Fachrureza<sup>1</sup>, Iraky Mardya Rakhmadhan<sup>2</sup>, <b>Muhammad Reyhan</b><sup>2</sup> and Adam Adam<sup>2</sup></p><p><sup>1</sup><i>Staff of Gastroentero-Hepatology Division, Internal Medicine Department, Dr. Saiful Anwar General Hospital, Malang, Indonesia;</i> <sup>2</sup><i>Indonesian Society of Internal Medicine Resident, University of Brawijaya, Malang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The study investigated the relationship between SCFA and butyrate acid levels and APRI and FIB-4 scores in hepatitis B and C-related cirrhosis.</p><p><b><i>Materials and Methods:</i></b> This cross-sectional study included 27 fecal samples from patients with cirrhosis related to hepatitis B and C in the gastroentero-hepatology clinic at Saiful Anwar General Hospital in Malang, East Java, where SCFA and butyrate acid levels were measured using a gas chromatography analyzer.</p><p><b><i>Results:</i></b> The study analyzed fecal SCFA levels among Hepatitis B and C patients using APRI scores between low-risk and intermediate-risk groups (9.18 ± 4.2 vs 12.40 ± 6.2, p=0.172), as well as butyrate levels (1.45 ± 1.09 vs 2.70 ± 1.88, p=0.056). In addition, the patients were categorized into low, intermediate, and high-risk groups based on their FIB-4 scores. However, there were no significant differences in butyrate levels (1.55 ± 1.18 vs 1.10 ± 0.28 vs 3.02 ± 2.01, p=0.067) or average fecal SCFA levels (9.6 ± 4.53 vs 7.80 ± 3.27 vs 12.75 ± 6.55, p=0.30) between these groups. The analysis suggests that fecal SCFA levels may vary among different groups of patients.</p><p><b><i>Conclusion:</i></b> The study found that liver fibrosis stages in hepatitis B and C patients were not significantly correlated by fecal SCFA and butyrate levels, possibly due to confounding factors like diets and medications history that were not being measured.</p><p><b>PP-02-227</b></p><p><b>Efficacy and safety of glucocorticoids therapy for severe fulminant viral hepatitis: a meta-analysis</b></p><p><b>Kailash Mani Pokhrel</b> and Kapil Khanal</p><p><i>Maharajgunj Medical Campus, Kathmandu, Nepal</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Severe fulminant viral hepatitis is associated with high mortality rates. The effectiveness of glucocorticoid therapy in this condition is debated across studies. Therefore, we conducted this meta-analysis to access the efficacy and safety of glucocorticoid therapy for severe fulminant viral hepatitis.</p><p><b><i>Materials and methods:</i></b> Adhering to PRISMA guidelines, we systematically searched Embase, PubMed, Scopus, and Google Scholar using specific search strategies for studies published in English up to July 9, 2024, comparing glucocorticoids with non-glucocorticoids treatments. The primary outcome was reduction in mortality rate, and secondary outcomes included improvement in liver function parameters and complications associated with glucocorticoid treatment.</p><p><b><i>Results:</i></b> Our meta-analysis included four randomized trials and 12 cohort studies, involving 2972 patients. Mortality was significantly lower in glucocorticoid treatment group compared to non-glucocorticoid group [Odds ratio (OR) = 0.41, 95% confidence interval (CI) 0.21 – 0.80, p = 0.009]. There was no significant difference in standardized mean differences (SMD) of total bilirubin and albumin levels between corticosteroid group and non-corticosteroid group before and after treatment. Bleeding [OR = 1.54, 95%CI 0.69 – 3.46, p = 0.29] and infection rates [ OR = 1.63, 95% CI 0.57 – 4.69, p = 0.36] were higher in the corticosteroid group, but these differences were not statistically significant.</p><p><b><i>Conclusion:</i></b> Glucocorticoid therapy for severe fulminant viral hepatitis significantly reduces the mortality without significant increase in bleeding or infection rates.</p><p><b>PP-02-228</b></p><p><b>Meta-analysis of hbv vaccine response in hemodialysis patients: impact of diabetes mellitus and hemoglobin level</b></p><p><b>Edgar Irvin Raranta</b><sup>1</sup>, Bradley Jimmy Waleleng<sup>2</sup>, Fandy Gosal<sup>2</sup>, Luciana Rotty<sup>2</sup>, Jeanne Winarta<sup>2</sup> and Andrew Waleleng<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>2</sup><i>Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This meta-analysis aims to assess and synthesize the existing evidence regarding the Hepatitis B Virus (HBV) vaccine response in hemodialysis patients, focusing on the impact of comorbid diabetes mellitus and hemoglobin levels.</p><p><b><i>Methods:</i></b> A comprehensive review of relevant studies was conducted from online databases PubMed, EMBASE, the Cochrane Library, and Google Scholar using terms like \"Hepatitis B vaccine\", “Response”, \"Diabetes mellitus\", and \"Hemoglobin\" for studies published up to August 2024. Duplicates were removed, and titles, abstracts, and full texts were screened for relevance. The review included studies that reported the impact of DM and hemoglobin levels on HBV vaccine response.</p><p><b><i>Results:</i></b> Out of 183 identified articles, 57 were screened and 40 were deemed relevant. We identified 8 studies involving 4,377 patients on HD. The findings indicate that patients with DM exhibit a significantly lower seroconversion rate compared to non-diabetic patients, highlighting the adverse effect of this comorbidity on HBV vaccine response. Additionally, patients with higher hemoglobin levels demonstrated improved seroconversion rates, suggesting that adequate hemoglobin may enhance immune response to the vaccine. Aggregation of study results showed a significant decrease in response rates among the diabetic patients [pooled OR=0.59 (95% CI 0.50-0.70)] and higher hemoglobin levels in responders [mean difference 0.40 (95%CI 0,25-0.54].</p><p><b><i>Conclusion:</i></b> Our meta-analysis showed a significant association between diabetes mellitus and hemoglobin levels with impaired response to HBV vaccine in HD patients. Future research should prioritize exploring optimized vaccination protocols and management of comorbidities to enhance protective immunity against HBV in HD patients.</p><p><b>PP-02-229</b></p><p><b>Association between vitamin d level and hepatitis b disease: a systematic review</b></p><p><b>Edgar Irvin Raranta</b><sup>1</sup>, Bradley Jimmy Waleleng<sup>2</sup>, Fandy Gosal<sup>2</sup>, Luciana Rotty<sup>2</sup>, Jeanne Winarta<sup>2</sup> and Andrew Waleleng<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;</i> <sup>2</sup><i>Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This systematic review aims to assess and synthesize the existing evidence regarding the relationship between vitamin D levels and clinical conditions in patients with hepatitis B.</p><p><b><i>Methods:</i></b> Following PRISMA guidelines, this review conducted a thorough search of PubMed, EMBASE, the Cochrane Library, and Google Scholar using terms like \"Hepatitis B,\" \"Vitamin D,\" and \"Liver Disease\" for studies published up to August 2024. Duplicates were removed, and titles, abstracts, and full texts were screened for relevance. The review included randomized controlled trials (RCTs) that reported serum vitamin D levels and their effects on HBV outcomes.</p><p><b><i>Results:</i></b> Out of 214 identified articles, 148 were refined to those with free full-text access, and 100 were deemed relevant. Five high-quality RCTs, published between 2014 and 2024, were included. These studies, involving diverse populations such as infants, chronic hepatitis B (CHB) patients, and HIV-infected adults, used various vitamin D measurement methods, mainly focusing on serum 25-hydroxyvitamin D (25(OH)D). The findings showed mixed effects of vitamin D on HBV outcomes, with some studies indicating no significant impact on HBV replication or vaccine response, while others highlighted the importance of adequate vitamin D levels before vaccination for optimal vaccine efficacy.</p><p><b><i>Conclusion:</i></b> The evidence regarding vitamin D and HBV infection is inconclusive. While some data suggest potential benefits, such as improved vaccine efficacy and immune modulation, other studies report no significant effects. Further research is needed to resolve these inconsistencies and determine the association betweel vitamin D and Hepatitis B.</p><p><b>PP-02-231</b></p><p><b>Acute Kidney Injury in Acute-on-Chronic Liver failure: Prognostic indicators and Clinical outcomes</b></p><p><b>Sumaswi Angadi</b><sup>1</sup>, Srujan Reddy<sup>1</sup>, Suprabhat Giri<sup>2</sup> and Sukanya Bhrugumalla<sup>1</sup></p><p><sup>1</sup><i>Nizam's Institute of Medical Sciences, Hyderabad, India;</i> <sup>2</sup><i>Kalinga Institute of Medical Sciences, Bhubaneswar, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The present study aims to analyze the progression of AKI in ACLF and identify predictive factors for mortality.</p><p><b><i>Materials and Methods:</i></b> This is a single-center, retrospective analysis of patients with ACLF and AKI admitted to a tertiary center in South India between January 2023 and April 2024. The diagnosis of ACLF was based on the Asia Pacific Association for the Study of Liver (APASL) definition. Clinical outcomes at 28 days and 90 days were recorded. Factors predicting mortality were analyzed using Receiver Operating Characteristic (ROC) curves. Survival analysis was performed using Kaplan-Meier and Cox regression models.</p><p><b><i>Results:</i></b> A total of 102 patients with ACLF and AKI were included in the study. In-hospital mortality was 36.27%, while mortality at 28 days was 38.24%, and at 90 days was 49.02%. Hepatorenal syndrome (HRS)- acute kidney disease (AKD) developed in 9.8%, and 1.96% developed HRS- chronic kidney disease (CKD). Non-survivors had a significantly higher APASL Research Consortium Score (AARC) score (p<0.001) and a higher stage of AKI compared to survivors (p<0.001). Also, HRS was common among survivors compared to Inflammation/sepsis-associated AKI among non-survivors (p=0.001). The AARC score had a high AUROC of 0.907, followed by the MELD score (AUROC:0.872), serum urea (AUROC:0.709), creatinine (AUROC:0.704) and bilirubin (AUROC: 0.697). Independent predictors of mortality were AARC score (HR:1.759, CI:1.331-2.325, p <0.001) and serum urea (HR:1.009, CI:1.002 - 1.016, p=0.016).</p><p><b><i>Conclusion:</i></b> AARC score and serum urea are better mortality predictors than serum creatinine, serum bilirubin, and MELD scores. Inflammation/Sepsis-associated AKI is common among non-survivors.</p><p><b>PP-02-232</b></p><p><b>Plasma Exchange for Acute-on-Chronic Liver Failure from Severe Alcoholic Hepatitis with Failed Steroid Therapy</b></p><p>Nagarajan Venkatchocku Ashok Annamalai<sup>1</sup> and Marianne Anastasia De Roza<sup>2</sup></p><p><sup>1</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Sengkang General Hospital, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Acute-on-chronic liver failure (ACLF) associated with multi-organ failure, has a high mortality burden. The mechanisms are thought to be driven by hyperactive inflammatory and immune responses triggered by microbial and non-microbial factors. Plasma exchange (PLEX) is a potential alternative to liver transplant that improves survival.</p><p><b><i>Case Report:</i></b> A 56-year-old man with Child-Pugh C11 alcoholic liver cirrhosis was admitted to the intensive care unit for Grade 3 ACLF, precipitated by severe alcoholic hepatitis. (Albumin 29 Bilirubin 414 ALP 133 ALT 40 AST 128 Na 123 Creatinine 487 INR 1.82) with MELD score of 39. He required inotropic support and renal replacement therapy(RRT).</p><p>Patient was covered with antibiotics and started on steroids for Maddrey Discriminant Function (MDF) score of 61.5. However, there was no improvement with Lille score 0.603 (>0.45) on day 5 and 0.535 on day 7, hence steroids were discontinued. Patient was not a liver transplant candidate due to alcoholism and poor social support. He was commenced on a trial of PLEX and completed 3 cycles with standard volume plasma replacement resulting in clinical and biochemical improvement. He was discharged from hospital with 30-day survival at outpatient review.</p><p><b><i>Discussion:</i></b> In summary, this highlights that PLEX is a potential treatment option in Alcoholic ACLF patients which would otherwise have a 1 month mortality of 74%, as it reduces systemic inflammatory reactions & modulates the adaptive immune response. This is especially important in view of Singapore’s organ scarcity and the limited transplant options for those without social support.</p><p><b>PP-02-233</b></p><p><b>Ancient Remedies, Modern Risks: Case Series of Acute Liver Failure Due to Traditional Complementary Medicine</b></p><p><b>Wah Loong Chan</b>, Yee Ling Tan, Kee Huat Chuah, Stanley Khoo and Wah Kheong Chan</p><p><i>University Of Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Traditional Complementary Medicine (TCM) is widely used in Asia and can lead to fulminant liver failure. This case series highlights severe hepatotoxicity associated with TCM use, even in patients without pre-existing liver disease, and emphasizes the importance of thorough medication history in evaluating unexplained liver injury.</p><p><b><i>Case series:</i></b> Case 1: A 41-year-old Thai woman with no past medical history developed acute liver failure (ALF) with elevated liver enzymes (AST 9660 U/L, ALT 1438 U/L), hyperbilirubinemia (273 μmol/L), hyperlactatemia (4.10 mmol/L), coagulopathy (INR 1.9), and hepatic encephalopathy after consuming TCM containing Borneolum Syntheticum for three months. Despite intensive care including N-acetylcysteine and plasma exchange (PLEX), she succumbed to complications as liver transplantation was not an option for her.</p><p><b><i>Case 2:</i></b> A 30-year-old healthy woman presented with ALF (AST 15,210 U/L, ALT 9,520 U/L), hyperbilirubinemia (463 μmol/L), and hepatic encephalopathy after taking unspecified TCM for three months. She fully recovered after two cycles of low-volume PLEX without needing liver transplantation.</p><p><b><i>Discussion and conclusion:</i></b> Both cases demonstrate severe hepatotoxicity associated with TCM use. TCM-induced liver injury can present as hepatocellular, cholestatic, or mixed patterns. This report emphasizes the importance of detailed medication history, including TCM, in evaluating unexplained liver injury. Increased awareness of TCM hepatotoxicity is necessary among practitioners and the public. Although both cases met the King's College Criteria for ALF, liver transplantation was limited. PLEX can be a bridging and definitive rescue therapy for selected ALF patients.</p><p><b>PP-02-234</b></p><p><b>Successful Treatment of Acute Liver Failure with Conservative Therapy in Young Adults</b></p><p><b>Putri Nurra Hakim</b> and Bella Monika Rajagukguk and Muhammad Yugo Hario Sakti Dua and Shinta Vera Renata Hutajulu and Franz Josef Vincentius Pangalila</p><p><i>Mayapada Hospital Jakarta Selatan, Jakarta, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Acute Liver failure (ALF) is a rapid loss of liver function that occurs in days or weeks without pre-existing liver disease. It can be caused by medications, autoimmune disease, metabolic disease, hepatitis and other viruses. This report presents the case of an 18-year-old female with acute liver failure following tuberculosis treatment and the role of supportive therapies in managing ALF.</p><p><b><i>Case Report:</i></b> An 18-year-old female presented to the emergency room with hepatic encephalopathy. Patient showed agitation, respiratory distress and decreased consciousness and was then transferred to the intensive care unit to be intubated. Patient had a history of lymphadenitis tuberculosis with treatment regimen of Rifampicin and Pyrazinamide. Diagnostic workup revealed very high level of AST and ALT, bilirubin, amylase, ammonia, and a reactive result of CMV IgG. Hepatitis C and hepatitis B marker were found to be non-reactive. Patient was given antibiotics and administration of L-ornithine-L-aspartate (LOLA), that reduce ammonia levels and improve mental status (GCS), and Stronger Neo-Minophagen C (SNMC), which provides a hepatoprotective effect and reduce ALT and AST levels. Patient showed good response with therapy, regained consciousness and extubated after four days. Patient was discharged to the ward the following day.</p><p><b><i>Discussion:</i></b> This case highlights the use of appropriate and prompt supportive therapies in managing ALF and the effectiveness of non-surgical approaches in stabilizing patients without invasive intervention. This report also indicates the crucial role of early recognition, prompt initiation of appropriate treatments and close monitoring in improving clinical outcomes for patients with acute liver failure.</p><p><b>PP-02-235</b></p><p><b>The Application of a kind of ROS-Sensitive Nanoparticles in the Treatment of Acute Liver Injury</b></p><p><b>ZiYi Peng</b></p><p><i>Department Of Gastroenterology And Hepatology, Tianjin Medical University, Tianjin, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>Acetaminophen (APAP),which is widely used all over the world and also prone to overdose, is the leading cause of acute liver injury(ALF).Meanwhile,reactive oxygen species (ROS) plays crucial role in the mechanisms of APAP-induced liver injury (AILI).Restoring liver functions to relieve acute liver injury by cutting off ROS overproduction is a potential strategy for ALF treatment.N-acetylcysteine (NAC) has been the only clinically available antidote but its therapeutic window is quite limited.</p><p>Therefore,we design a kind of single atom nanoparticles which almost accumulate in the liver and exert reactive oxygen species scavenging function.</p><p>Moreover,this kind of ROS-Sensitive nanoparticles also plays a positive role in responding to inflammation,cell apoptosis, mitochondrial damage,and regenerative disorders studied in vivo and in vitro,which are other relative mechanisms mediating ALF.Considering the superior performance of combing nanomedicine and hepatology, SeNPs therapeutic effect and good biocompatibility may provide an effective alternative for further clinical treatment of AILI.</p><p><b>PP-02-236</b></p><p><b>The Effectiveness of Heparin in Preventing Liver Failure in Post-Hepatectomy Patients: An Evidence-Based Case Report</b></p><p><b>Rizki Trismimanda</b><sup>1</sup>, Fhathia Avisha<sup>2</sup>, Crisdina Suseno<sup>3</sup> and Masyfuk Zuhdi Jamhur<sup>4</sup></p><p><sup>1</sup><i>General Practitioner, Kudungga District Hospital, Kutai Timur, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Ciptomangukusumo Hospital, Jakarta, Indonesia;</i> <sup>3</sup><i>General Practitioner, Mentawai Island District Hospital, Mentawai Island Regency, Indonesia;</i> <sup>4</sup><i>General Practitioner, Medical Faculty of Andalas University, Padang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Post-hepatectomy liver failure (PHLF) is a significant complication of hepatectomy procedure in the first few days after surgery. Heparin is an anticoagulant that protects endothelial cells, prevents thrombosis in hepatic vessels, reduces hepatic hemodynamic abnormalities, and significantly decreases hepatocyte apoptosis due to hemorrhagic and reperfusion injuries. However, the effectiveness of using heparin as a prophylactic therapy in liver surgery remains debated and requires further research.</p><p><b><i>Objective:</i></b> To determine the effectiveness of heparin in preventing liver failure after hepatectomy or liver resection.</p><p><b><i>Methods:</i></b> A literature search was conducted on four databases, namely PubMed, Cochrane, Scopus, and ScienceDirect on June 27, 2024, using the keywords “Heparin” OR “Unfractionated heparin” OR “Anticoagulant” AND “Post Hepatectomy” OR “Liver Resection” AND “Liver Failure”. There were 0 studies on PubMed and Cochrane, 1 study on Scopus, and 9 on ScienceDirect. The articles found were screened based on the inclusion and exclusion criteria. Critical reviews were conducted using the Critical Appraisal Skills Programme for Cohort Studies.</p><p><b><i>Results:</i></b> Heparin showed a significant association with a decreased risk of PHLF (OR: 0.518; 95% CI: 0.295-0.910; P=0.022). Furthermore, heparin therapy was linked to improved short-term postoperative outcomes, such as shorter ICU stays, decreased need for respiratory support and CRRT, lower occurrences of hypoxemia, and ICU mortality.</p><p><b><i>Conclusion:</i></b> Heparin administration in post-hepatectomy patients decreases the occurrence of liver failure and improves short-term postoperative outcomes. Heparin therapy may be a valuable prophylactic treatment for preventing PHLF in post-hepatectomy patients.</p><p><b><i>Keywords:</i></b> Hepatectomy, Heparin, Liver Resection, Post Hepatectomy Liver Failure</p><p><b>PP-02-237</b></p><p><b>Decoding the Crosstalk: Neutrophil Extracellular Traps and CD8+ T Cells in Autoimmune Hepatitis</b></p><p><b>Shuhui Wang</b>, Yu Chen, Yu Lei, Dean Tian and Han Wang</p><p><i>Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> CD8+ T cells cluster in the liver during autoimmune hepatitis, leading to ongoing disease and relapse after treatment stops. High levels of neutrophil extracellular traps are found in the livers of AIH patients and models, but their relationship with CD8+ T cells remains unclear.</p><p><b><i>Materials and Methods:</i></b> The chronic AIH mouse model was developed and progressed through infection of AAV9-CYP2D6. Depletion of NETs was accomplished by utilizing PAD4 knockout (KO) mice. The mechanism by which NETs regulate the functions of CD8+ T cells was explored through RNA sequencing. Flow cytometry and immunofluorescence analyses were conducted to investigate the interaction between NETs and CD8+ T cells.</p><p><b><i>Results:</i></b> In the context of AIH in the liver, there was a positive correlation between the presence of NETs and CD8+ T cells. Reduction of NETs was found to impede the progression of AIH and the infiltration of CD8+ T cells. Analysis of RNA sequencing data indicated that NETs influenced the mobility and cytotoxicity of CD8+ T cells. Flow cytometry analysis further confirmed that NETs hindered the migration and cytokine-mediated cytotoxicity of CD8+ T cells, while also entraped these cells. In vivo experiments with PAD4 KO mice showed that inhibiting NETs reduced the cytotoxic activity of CD8+ T cells.</p><p><b><i>Conclusion:</i></b> NETs can drive AIH progression by entrapping and affecting CD8+ T cells, leading to increased cytotoxic activity and promoting crosstalk between innate and adaptive immunity in the liver. Targeting NETs and CD8+ T cell interactions may be a promising treatment approach for AIH.</p><p><b>PP-02-238</b></p><p><b>Imaging findings of massive splenic infarction that occurred before liver transplantation: a case report</b></p><p><b>Ki Choon Sim</b>, Min Ju Kim, Beom Jin Park, Deuk Jae Sung, Na Yeon Han and Yeo Eun Han</p><p><i>Department of Radiology, Korea University Anam Hospital, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Splenic infarction, often linked to hemato-oncological, infectious, immunological conditions, or ischemia, occurs when the spleen's arterial or venous circulation is compromised. We present the case of a 56-year-old male with alcoholic liver cirrhosis who developed massive intraabdominal bleeding and hepatorenal syndrome preoperatively, and successfully underwent liver transplantation (LT).</p><p><b><i>Case Description:</i></b> The patient was transferred to our tertiary institution due to worsening refractory ascites and recently developed intraabdominal bleeding. A CT scan confirmed hemoperitoneum, and successful embolization was performed on the bleeding cystic artery branch. Retrospective CT review showed a large, indistinct low-density area in the spleen, but this was initially missed. However, no physician, including the radiologist, was aware of the spleen issue at that time. Subsequently, living donor LT was performed using a modified right lobe graft of the orthotopic standard type, and splenic artery ligation was not performed. On postoperative day 3, a CT follow-up revealed a massive splenic infarction was recognized for the first time on imaging. Subsequently, complicated fluid collection that could not be controlled with conservative treatment continued in the perisplenic area. Therefore, an exploratory laparotomy, including splenectomy, was performed 48 days after the LT. Histopathology showed the spleen was hemorrhagic and necrotic appearance with cystic change, consistent with splenic infarction.</p><p><b><i>Discussion:</i></b> We hypothesize that the massive splenic infarction most likely resulted from hypoperfusion and a hypercoagulable state before liver transplantation, and authors believe it had a negative impact on the patient's recovery after the liver transplantation.</p><p><b>PP-02-239</b></p><p><b>Characteristics of patients referred to a specialized fatty liver clinic in Abu Dhabi</b></p><p>Heba Abualkas and <b>Mahmoud Kiblawi</b> and Wala Hamed and Omar Khaddam and Ahmad Alrifai</p><p><i>Sheikh Shakhbout Medical City Ssmc, Abudhabi, United Arab Emirates</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To highlight the characteristics of the patients referred to the first specialized clinic for steatotic liver disease in Abu Dhabi, Sheikh Shakhbout Medical City during the period between 2018 to 2020.</p><p><b><i>Method:</i></b> This is a retrospective cohort study on patients referred to fatty liver clinic with abdominal ultrasound showing fatty liver. All data were obtained from the electronic outpatient records. Individual data was collected for patients including comorbidities, relevant laboratory values amongst others. All patients had assessment for fibrosis by usual scores and VCTE( vibration controlled transient elastography).</p><p><b><i>Result:</i></b> A total of 444 patients were included in the study with male representing 39.4%. we found that 40.% were diabetic, 65% patients were obese , 25 % were hypertensive, 44 % has dyslipidemia and 3.4% had cardiovascular disease. 28% of patients has more than1component of metabolic syndrome. 0.9% on presentation had NASH cirrhosis. using fib4 score17% of the patients were consider to have significant fibrosis (FIB 4>1.4). All patients had liver stiffness measurement using VCTE. 13.3% of the patients were classified as significant fibrosis (LS >8 KPA) of them 53% diabetic and 31% obese.</p><p><b><i>Conclusion:</i></b> Our cohort showed that majority of patients attending the fatty liver clinic fulfill the criteria of MASLD( metabolic dysfunction associated liver disease). Significant percentage of them had advanced fibrosis on presentation and 1 % already cirrhotic. Patients with advanced fibrosis has high prevalence of diabetes and obesity and these findings support screening in this high risk population.</p><p><b>PP-02-240</b></p><p><b>Characteristics of Metabolic Dysfunction Associated Steatotic Liver Disease patients with advanced fibrosis assessed by fibroscan</b></p><p>Heba Abualkas and <b>Mahmoud Kiblawi</b> and Wala Hamed and Omar Khaddam and Ahmad Alrifai</p><p><i>Sheikh Shakhbout Medical City SSMC, Abudhabi, United Arab Emirates</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To study the characteristics of patients who underwent a vibration controlled transient elastography (VCTE) fibroscan assessment in fatty liver clinic. The goal is to compare patients with an advanced fibrosis score of F3–F4 with the group of patients with a score of F0–F2.</p><p><b><i>Methods:</i></b> This is a retrospective review of the electronic medical records of adult patients who had fibroscan during 2018 to 2020, total of 399. we looked at various variables including demographics, comorbidities, and relevant laboratory values amongst others. Wilcoxon rank sum test was used to compared continuous and ordered data. Fisher’s exact test was used to compare categorical data.</p><p><b><i>Results:</i></b> The study included 399 patients who underwent a VCTE assessment. Patients with advanced fibrosis (F3-F4) were 7.5% of the total study population. The median age of patients with score of F3-F4 was 52 years of age as compared to patients with score of F0-F2 which was 47, this difference was statistically significant (p=0.034). Patients classified as F3-F4 on Fibroscan were more likely to have dyslipidemia, diabetic, and obese. Moreover they tend to have cirrhosis on ultrasound, higher Non Alcoholic Fatty Liver Disease (NAFLD) score, higher Fibrosis Index Based on 4 factors (FIB-4) score, and higher Aspartate Aminotransferase to Platelet Ratio Index (APRI) score.</p><p><b><i>Conclusion:</i></b> Patients with features of metabolic syndrome and with high fibrosis score tend to have advanced fibrosis in our studied population. This highlights the importance of early screening and implementation of prevention strategies in our population to prevent disease progression.</p><p><b>PP-02-241</b></p><p><b>Risk of liver fibrosis in patients on methotrexate with coexisting metabolic and other risk factors</b></p><p><b>Shiran Appuhamy</b><sup>1</sup>, Chamila Ranawaka<sup>1</sup>, Suranjan Viraj<sup>1</sup>, Sakshara Ranasinghe<sup>1</sup>, D M Amaratunga<sup>1</sup> and Duminda Munidasa<sup>2</sup></p><p><sup>1</sup><i>Colombo North Teaching Hospital, Ragama, Sri Lanka;</i> <sup>2</sup><i>Rheumatology and Rehabilitation Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The study aims to evaluate the risk of liver fibrosis in patients undergoing long-term Methotrexate (MTX) therapy for rheumatoid arthritis (RA) and psoriasis, considering the influence of metabolic and other risk factors including diabetes mellitus, hypertension, dyslipidaemia, alcohol use, and Ayurvedic medication use.</p><p><b><i>Materials and Methods:</i></b> We analysed clinical data from 206 patients on MTX for rheumatoid arthritis or psoriasis for more than 6 months. Stage of liver fibrosis was assessed by transient elastography (fibroscan). Ordinal regression and correlation analyses were performed to investigate the relationships between liver fibrosis and the specified risk factors.</p><p><b><i>Results:</i></b> Descriptive statistics revealed that 73.8% of patients were on MTX therapy, with 78.2% having RA and 21.8% having psoriasis. The ordinal regression analysis indicated that MTX use is associated with a lower risk of advanced liver fibrosis stages (coefficients ranging from -0.354 to -0.163 across thresholds). Correlation analysis showed a weak negative correlation between MTX use and liver fibrosis (r = -0.108). Positive correlations were observed between liver fibrosis and alcohol use (r = 0.225), diabetes (r = 0.231), hypertension (r = 0.131), and dyslipidaemia (r = 0.139).</p><p><b><i>Conclusion:</i></b> The study suggests that long-term MTX use in patients with RA or psoriasis is not associated with a significant risk of advanced liver fibrosis. However, coexisting metabolic conditions and alcohol use, significantly increase the risk of liver fibrosis. These findings underscore the importance of monitoring liver health and managing metabolic risk factors in patients undergoing MTX therapy for RA and Psoriasis.</p><p><b>PP-02-242</b></p><p><b>Factors Influencing Liver Steatosis and Fibrosis: A Descriptive and Regression Analysis</b></p><p><b>Shiran Appuhamy</b>, Chamila Ranawaka, Suranjan Viraj and Sakshara Ranasinghe</p><p><i>Colombo North Teaching Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to identify the factors influencing liver steatosis and fibrosis, focusing on age, gender, BMI, alcohol use, Ayurvedic medication history, diabetes mellitus, hypertension, and dyslipidaemia.</p><p><b><i>Materials and Methods:</i></b> Data, including demographic and clinical variables were collected from 204 patients who underwent transient elastography (fibroscan) and analysed. Steatosis and fibrosis stages were classified using established criteria. Regression analysis was performed to determine the relationship between selected factors and liver conditions. Descriptive statistics and correlation analyses were used to elucidate gender differences and the impact of BMI and age.</p><p><b><i>Results:</i></b> Descriptive analysis showed a median age of 55 years and a mean BMI of 25.31. Males comprised 60.29% of the cohort. The most severe steatosis stage (S3) was more prevalent in males (47.15%) compared to females (43.21%). Advanced fibrosis (cirrhosis) was slightly higher in males (15.45%) than females (12.35%). The regression analysis revealed that BMI (p<0.001) and dyslipidaemia (p=0.039) positively influenced liver steatosis. The model explained 16.1% of the variance (R²=0.161). For fibrosis, diabetes (p=0.005) was positively associated, explaining 12.7% of the variance (R²=0.127).</p><p><b><i>Conclusion:</i></b> BMI and dyslipidaemia significantly influence liver steatosis, while DM is a key factor in liver fibrosis. The study highlights the importance of implementing lifestyle and medical interventions focusing on weight reduction, diabetes control and management of dyslipidaemia to mitigate liver disease progression.</p><p><b>PP-02-243</b></p><p><b>Prevalence of liver fibrosis in patients with ultrasound-proven fatty liver disease and chronic liver disease</b></p><p><b>Shiran Appuhamy</b>, Chamila Ranawaka, Suranjan Viraj and Sakshara Ranasinghe</p><p><i>Colombo North Teaching Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to assess the prevalence and severity of liver fibrosis in patients with ultrasound evidence of either Fatty Liver Disease (FLD) or Chronic Liver Disease (CLD).</p><p><b><i>Materials and Methods:</i></b> We evaluated 1533 patients with ultrasound-diagnosed FLD or early CLD by using transient elastography (fibroscan) from October 2021 to May 2024. Data were analysed to determine the distribution of steatosis and fibrosis stages in both patient groups. Steatosis and fibrosis stages were classified using established criteria.</p><p><b><i>Results:</i></b> Among FLD patients, 56.04% had severe steatosis (S3), 19.04% had moderate steatosis (S2), and 15.07% had no steatosis (S0). In CLD patients, 43.24% had no steatosis (S0), 24.32% had severe steatosis (S3), and 23.42% had moderate steatosis (S2). Considering fibrosis, 39.98% of FLD patients had no fibrosis (F0), 21.61% at F2, and 14.98% at F3. In CLD patients, 53.15% found to have advanced cirrhosis, 17.12% at F4, and 12.61% at F3.</p><p><b><i>Conclusion:</i></b> The findings reveal a significant prevalence of severe steatosis in patients with ultrasound-diagnosed FLD. Many FLD patients remain at early fibrosis stages (F0), yet a notable proportion show significant fibrosis (F2 and F3) highlighting the need for early detection and management to prevent progression. In contrast, CLD patients exhibit a high prevalence of advanced fibrosis and cirrhosis, reflecting the chronic and progressive nature of the disease. Monitoring of patients with ultrasound evidence FLD or CLD using transient elastography is pivotal for early fibrosis detection, enabling better disease management and prevention of progression cirrhosis.</p><p><b>PP-02-244</b></p><p><b>Performance of FibroTouch<sup>®</sup> in Assessing Hepatic Steatosis and Fibrosis for Metabolic Dysfunction-Associated Steatotic Liver Disease</b></p><p><b>Roongruedee Chaiteerakij</b> and Soe Thiha Maung and Thanikan Sukaram and Yuda Chongpison and Chonlada Phathong</p><p><i>Roongruedee Chaiteerakij, Associate Professor, Chulalongkorn University, Bangkok, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> FibroTouch® has shown efficacy in staging hepatic fibrosis in patients with chronic viral hepatitis B, but its performance in assessing liver steatosis and fibrosis in metabolic dysfunction-associated steatotic liver disease (MASLD) patients remains understudied. We aimed to evaluate the diagnostic performance of FibroTouch® in assessing steatosis and fibrosis in MASLD population.</p><p><b><i>Materials and Methods:</i></b> Liver stiffness measurements and steatosis were assessed using FibroTouch® and FibroScan®, with FibroScan® as the reference standard. Pearson’s correlation test evaluated correlations, and kappa statistics determined agreement between two methods. Optimal cut-off values of FibroTouch® for predicting hepatic steatosis and fibrosis stages were determined through ROC curve analysis, with Youden index method.</p><p><b><i>Results:</i></b> Strong correlations were observed between FibroTouch® UAP and FibroScan® CAP (rho=0.74) and LSM values (rho=0.87) (p<0.001 for both) in total 380 patients. The mean CAP value for the entire cohort was 285±51 dB/m, and the median LSM for the cohort was 5.3kPa. The optimal FibroTouch® UAP cutoffs were 229 dB/m for S0 vs. S1, 267 dB/m for S1 vs. S2, and 294 dB/m for S2 vs. S3. For FibroTouch® LSM, the optimal cutoffs were 6.0kPa for F0-F1 vs. F2, 7.9kPa for F2 vs. F3, and 10.6kPa for F3 vs. F4. Moreover, FibroTouch® effectively assessed hepatic steatosis and fibrosis in patients with different BMIs.</p><p><b><i>Conclusion:</i></b> FibroTouch® proved valuable in assessing hepatic steatosis and liver fibrosis staging in MASLD patients, enhancing its applicability in various clinical settings as a suitable and convenient option for MASLD patients.</p><p><b>PP-02-245</b></p><p><b>Phenotyping of patients admitted in the past year in a colombian referral center in ibd</b></p><p><b>Viviana Parra-izquierdo</b> and Ginary Orduz and Silvia Hhoyos and Andrea Reatiga and Oscar Pinto and Juliep Sarmiento and Carlos Cuadros and Oscar Pinto and Alexis Duarte and Johon Garces and Juan Acevedo and Juan Frias and Cristian Florez</p><p><i>Leuven University, Leuven, Belgium</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> In Colombia there are currently 5 centers of excellence certified by the Pan American Crohn's and Colitis Organization and one of them is the only one that is certified and serves the population of eastern Colombia.</p><p><b><i>Objective:</i></b> Description of the cohort of patients who have been admitted in the last year to a Colombian Center of of Excellence in Inflammatory Bowel Disease.</p><p><b><i>Materials and Methods:</i></b> Descriptive observational cross-sectional study of patients diagnosed with pediatric and adult diagnosed pediatric and adult patients with Inflammatory Bowel Disease who have been admitted to a Center of Excellence.</p><p><b><i>Results:</i></b> Within the cohort of patients of the Center of Excellence in Inflammatory Bowel Disease, there are 90 patients, 56.6% of whom are women. Intestinal Disease, there are 90 patients of which 56.6% are omen. The average age is 40.8 years, with a minimum age of 7 years and a maximum age of 80 years, with 10 patients of pediatric age. The 68.8% are Ulcerative Colitis (37% pancolitis, 54.8% left colitis and 8% proctitis) and 31.1% Crohn's Disease (64.2% ileal, 28.5% ileocolonic and 7.14% colonic) with 17.85% with fistulizing and stenosing involvement. In Ulcerative Colitis 27.41% are receiving biologic therapy, infliximab being the most used biologic 47%, adalimumab 23.5%, vedolizumab 23.5% and 5.8% golimumab 5.8%.</p><p><b><i>Conclusions:</i></b> This study provides valuable information on inflammatory bowel disease in eastern Colombia, showing that there is a predominance of ulcerative colitis with greater use of biologic therapy</p><p><b>PP-02-246</b></p><p><b>TrkB mitigated liver fibrosis through regulating macrophage recruitment mediated by the TGF-β/c-Fos/CCL2 axis</b></p><p><b>Yueying Chen</b><sup>1,2</sup>, Jiayi Wei<sup>1,2</sup>, Shuxuan Li<sup>1,2</sup>, Kefan Yin<sup>1,2</sup>, Heming Wang<sup>1,3</sup>, Ling Dong<sup>1,2</sup>, Guangqi Song<sup>1,3</sup>, Qunyan Yao<sup>1,2</sup> and Xizhong Shen<sup>1,2</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Shanghai Institute of Liver Diseases, Shanghai, China;</i> <sup>3</sup><i>Joint Laboratory of Biomaterials and Translational Medicine, Puheng Technology, Suzhou, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Liver fibrosis is a main indicator for increased mortality and long-term comorbidity in nonalcoholic steatohepatitis. Our prior research has established that TrkB overexpression in hepatic stellate cells inhibited the TGFβ/SMAD signaling and alleviated hepatic fibrosis. This study aimed to delineate the role of TrkB overexpression in hepatocytes for liver fibrosis.</p><p><b><i>Materials and Methods:</i></b> Using the GEO database and liver samples with liver fibrosis, we investigated the expression of TrkB in different stages of liver fibrosis and normal live. The in vivo and in vitro models and 3D liver fibrosis organoids, single-cell sequencing, RNA-seq, CHIP-qPCR were used to elucidate the role and mechanism of TrkB in hepatocytes.</p><p><b><i>Results:</i></b> In 3D liver organoids and mice with elevated TrkB level in hepatocytes, the liver fibrosis and the inflammatory and fibrotic gene expressions was inhibited. The hepatocytes with TrkB overexpression inhibited the proliferation and activation of HSC. A diminished infiltration of hepatic macrophages in AAV8-TrkB-injected mice and3D liver organoids. The liver biopsy specimens illustrated a negative correlation between TrkB expression and infiltrated macrophage.</p><p>The CCR2 receptor signaling pathway in the liver of AAV8-TrkB-injected mice, with a decreased expression of CCL2. Additionally, TrkB suppressed the TGF-β/Smad3 signaling pathway in hepatocyte and the secretion of CCL2.</p><p>We identified the overexpression of FOS could significantly increase the level of CCL2 and reverse the inhibitory effect of TrkB on it.</p><p><b><i>Conclusion:</i></b> In conclusion, our study delineated that TrkB in hepatocytes attenuates liver fibrosis and the hepatic macrophage recruitment, through the TGF-β/Smad3/c-FOS/CCL2 signaling pathway.</p><p><b>PP-02-247</b></p><p><b>Bariatric endoscopy improves liver related parameters in MASLD – a systematic review and meta-analysis</b></p><p><b>Nathanael Chong</b><sup>1</sup>, Jian Shiun Tan<sup>2</sup>, George Boon Bee Goh<sup>3,4</sup>, Ravishankar Asokkumar<sup>3</sup> and Mark CC Cheah<sup>3</sup></p><p><sup>1</sup><i>Yong Loo Lin School Of Medicine, Singapore;</i> <sup>2</sup><i>Department of Internal Medicine, Singapore General Hospital, Singapore;</i> <sup>3</sup><i>Department of Gastroenterology and Hepatology, Singapore General Hospital, Singapore;</i> <sup>4</sup><i>Duke-NUS Medical School, National University of Singapore, Singapore</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> While bariatric endoscopy (BE) has emerged as a less invasive modality for weight loss, which is also a cornerstone of treatment for MASLD, the benefits of BE in MASLD remains to be clarified. We conducted a systematic review and meta-analysis to explore the potential impact of bariatric endoscopy in the context of MASLD</p><p><b><i>Methods:</i></b> MEDLINE, Embase and Web of Science were searched using terms related to MASLD and BE. Studies of MASLD patients who underwent endoscopic sleeve gastrectomy, intra-gastric balloon, duodenal mucosal resurfacing, POSE endosleeve were included. Metabolic and liver related parameters at 6- and 12-months post intervention were compared using a random effects model with data pooling by inverse variance weighting.</p><p><b><i>Results:</i></b> We included 14 studies in the meta-analysis with a total cohort size of 436 patients. At 6 months, there was histological improvement measured by the NAFLD activity score (MD = -2.49, 95%CI:[-2.99, -1.97], p = 0.011, I2=0) and no significant worsening of fibrosis stage (MD=0.21, 95%CI:[-1.61, 2.03], p=0.380, I2=0). There were reductions in the controlled attenuation parameter (MD = -46.99dB/m, 95%CI:[-67.29, -26.69], p = 0.003, I2=38.1), ALT (MD = -19.58, 95%CI:[-30.75, -8.42], p = <0.001, I2=65.5) and HbA1c (MD = -0.74%, 95%CI:[-1.20, -0.28], p=0.009, I2=0). Reductions in BMI were observed (MD =-4.08, 95%CI:[-4.95, -3.21] p=<0.001, I2=0) and sustained at 12 months (MD=-6.43, 95%CI:[-6.89, -5.98] p=<0.001, I2=0).</p><p><b><i>Conclusion:</i></b> Our study demonstrates that BE is an effective therapy for overweight/obese patients with MASLD, evidenced by improvements in several surrogates of liver and metabolic parameters with durable weight loss.</p><p><b>PP-02-248</b></p><p><b>Association between triglyceride, il-6 levels in non alcoholic fatty liver disease patients kandou hospital manado</b></p><p><b>Ari Christian</b>, Jimmy, Bradley Jimmy Waleleng, Luciana Rotty, Fandy Gosal, Andrew Waleleng and Jeanne Winarta</p><p><i>Division of Gastroenterology and Hepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Circulating levels of interleukin (IL)-6, a well-known inflammatory cytokine. Elevated IL-6 levels has been reported to be associated with non-alcoholic fatty liver disease (NAFLD) and also triglyceride levels. This study aims to assess the association between triglyceride and IL-6 levels in NAFLD patients.</p><p><b><i>Material and Methods:</i></b> A cross-sectional study involving all NAFLD patients in the outpatient ward of the Internal Medicine Department at Kandou Hospital Manado was conducted from January to July 2024. Aged 18–60 years were included in the study. Patients with other cardiovascular infection and Alcoholic were excluded. Subjects meeting the inclusion and exclusion criteria were included in the study until the required sample size was achieved. After data collection, statistical analysis was performed using SPSS version 25.0. Fisher's Exact and Pearson correlation test was used to analyze the association between triglyceride and IL-6 levels. The p-value of ≤0.05 was considered significant.</p><p><b><i>Results:</i></b> A total of 48 subjects were included in the study, most were males (52.1%) with mean age of 49.77±11.05 years. Most of the subjects had triglyceride levels of ≤200 mg/dl and IL-6 leels od ≤5 pg/ml. Fisher's Exact test did not show significant association between triglyceride and IL-6 levels (p=0,666). No significant correlation was found between triglyceride and IL-6 levels (p=0,321, r=-0,146). This might be due to several confounding factors which is not adjusted in this study, i.e. comorbidities and history of taking medication for hypertriglyceridaemia.</p><p><b><i>Conclusion:</i></b> There is no association between triglyceride and IL-6 levels in NAFLD patients in Kandou Hospital Manado.</p><p><b>PP-02-249</b></p><p><b>Identification of hereditary PROS1 variation in a patient with non-cirrhotic portal vein thrombosis by WGS</b></p><p><b>Yingjie Ai</b>, Sitao Ye, Xiaoquan Huang and Shiyao Chen</p><p><i>Zhongshan Hospital, Shanghai, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Genetic factors could account for a subset of patients with portal vein thrombosis (PVT), especially non-cirrhotic patients. Protein S (PS) inactivates factor Va and VIIa and its deficiency is associated with increased risk of venous thromboembolism. PROS1 encodes PS and its variation was often detected in hereditary PS deficiency. Identification of PROS1 variation may explain the occurrence of PVT in non-cirrhotic patients.</p><p><b><i>Case Description:</i></b> The patient was a 27-year-old man with PVT and PS deficiency. In September 2022, he was presented to emergency because of acute upper abdominal colic pain. Ultrasound and CT showed thrombosis formation in portal vein, superior mesenteric vein, right distal brachial vein to forearm segment of the cephalic vein, and left forearm segment of the cephalic vein. Autoimmune diseases, blood system disease or malignancy were excluded. WGS revealed a proven pathogenic missense variant of PROS1 (c.1543C>T). The amino acid substitution R515C is presumed to result in unstable PS protein which is degraded intracellularly. Consistent with WGS result, he had a decreased protein S activity (18%). His mother also presented identical PROS1 variation while his father did not. The patient was treated with LMWH and then switched to rivaroxaban. In September 2023, re-examination by ultrasound showed thrombus recanalization.</p><p><b><i>Discussion:</i></b> Our study applied WGS as a precise and accurate method to identify the genetic factors underlying non-cirrhotic PVT. Anticoagulation therapy could be recommended for patients with PVT caused by PROS1 mutations.</p><p><b>PP-02-250</b></p><p><b>A case of Portal Vein Thrombosis in a Patient with Breast Cancer</b></p><p><b>Yujin Kim</b></p><p><i>Cheju Halla General Hospital, Jeju-si, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Portal vein thrombosis is an uncommon complication in a patient with breast cancer. Malignancy and several drugs are known to have prothrombic effects. We introduce a case of a patient with history of metastatic breast cancer under treatment with aromates inhibitor, and developed ascites and esophageal varices due to portal vein thrombosis.</p><p><b><i>Case Description:</i></b> A 47-year old female patient was referred from the oncology department with six months of abdominal distension. She has been treated for breast cancer for 6 years with surgery, chemotherapy, and radiotherapy.</p><p>An aromatase inhibitor, letrozole has been used for years.</p><p>Physical examination revealed distended abdomen and shifting dullness.</p><p>Initial blood test revealed platetet count 90,000/mL, AST/ALT 34/17 U/L, albumin 3.1 g/dL, and prothrombin time 13.4 sec. Serology test for hepatitis B and C virus infection was negative.</p><p>No abnormal result related with thrombophilia was found.</p><p>F2 esophageal varices without red color signs were seen on gastroscopy.</p><p>Diagnostic paracentesis was performed. The serum-ascites albumin gradient was 2.5g/dL, and ascites protein level was 1.1 g/dL. No malignant cell was found from the ascites.</p><p>The patient was diagnosed with pre-hepatic portal hypertension due to portal vein thrombosis associated with breast cancer.</p><p>Diuretics including furosemide and spinorolactone was given to control ascites.</p><p><b><i>Discussion:</i></b> Malignancy is a hypercoagulable state.</p><p>An aromatase inhibitor also can raises the risk for venous thromboembolism.</p><p>Development of portal vein thrombosis should be an important differential diagnosis when patients with history of malginancy and under treatment of drugs raise thromboembolic event developed new onset ascites.</p><p><b>PP-02-251</b></p><p><b>Portal Hypertension Bleeding Caused by Diffuse Liver Metastastic of Pancreatic cancer: a Case Report</b></p><p><b>Meti Metiani</b><sup>1</sup>, Dolvy Girawan<sup>2</sup>, Muhammad Begawan Bestari<sup>3</sup>, Nenny Agustanti<sup>4</sup> and Eka Surya Nugraha<sup>5</sup></p><p><sup>1</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, West Java;</i> <sup>3</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>4</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;</i> <sup>5</sup><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Portal hypertension due to diffuse spread of pancreatic cancer to the liver is rare. Massive secondary cancer to the liver results in portal flow resistance, changes in liver architecture, and mechanical compression of tumor infiltration. We report a case of variceal bleeding due to portal hypertension caused by extensive liver metastases in pancreatic cancer.</p><p><b><i>Case Description:</i></b> A 55-year-old woman came to the clinic with complaints of black tarry stools for three days. The patient was diagnosed with pancreatic cancer and had a history of gastrointestinal bleeding five years ago. There was no history of chronic liver disease. The liver function was within the normal limit. An enhanced computerised tomography scan of angiography and venography showed the portal vein was partially dilated; there was visible portosystemic collateral formation and multiple massive masses throughout the liver lobes. Endoscopy results showed large fundal varices and grade one esophageal varices.</p><p><b><i>Discussion:</i></b> Non-cirrhotic portal hypertension in liver metastases without chemotherapy occurs because of the presence of regenerating nodular hyperplasia, extensive stromal fibrosis, and diffuse tumor infiltration, which causes vascular compression. The prevalence of portal hypertension in liver metastases is unknown. Data on clinical features, management, and prognosis are limited. There are currently no diagnostic criteria, primarily based on imaging techniques. Rapid diagnosis and appropriate monitoring are needed to prevent complications of variceal bleeding or hepatic encephalopathy.</p><p><b>PP-02-252</b></p><p><b>Acute hemodynamic response to Carvedilol in cirrhotic children with clinically significant portal hypertension</b></p><p><b>Deepika Yadav</b>, Rajeev Khanna, Seema Alam, Vikrant Sood and Bikrant Bihari Lal</p><p><i>Department of Pediatric Hepatology, Institute of Liver and Biliary sciences, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To study the proportion of children (2-18year age) with acute hemodynamic response in those with clinically significant portal hypertension (CSPH) and its predictors in cirrhotic children with CSPH.</p><p><b><i>Material and Methods:</i></b> Under-18-age cirrhotic children underwent HVPG via the transjugular route. Those with CSPH were administered a single dose of carvedilol (0.2 mg/kg) via oral or nasogastric route. AHR was defined as a decrease in HVPG ≥20% from baseline or absolute HVPG value declining to ≤12 mm Hg, 90 min after carvedilol.</p><p><b><i>Results:</i></b> Thirty-eight children (63% males) with median age of 12.7 years (IQR11, 15years) with CSPH were included. Autoimmune liver disease (55%) was the commonest etiology. Median PELD and Child-Pugh scores were 11(IQR 2, 19) and 8(IQR 5, 10).Twenty-one (55%) had prior decompensation– 4 had variceal bleed;18 (47%) had clinically significant varices. The median baseline HVPG was 17 mm Hg (IQR15, 22mm Hg). Following 90 minutes of carvedilol, 18 (47%) patients had an AHR. On multivariate analysis, AHR in HVPG was predicted by portal vein size[Exp(B)=0.492;95% CI=0.289-0.838, P=0.001] and serum sodium [Exp(B)=1.407; 95% CI=1.004-1.971, P=0.033], but not by other demographic, clinical, laboratory, or endoscopic variables. Children who did not achieve AHR had poor SNL at 6 months (OR = 2.308; 95% CI 1.53-3.47, p=0.19) – there were 4 deaths and 2 liver transplants at 6 months of follow-up.</p><p><b><i>Conclusions:</i></b> Around half of the children achieved AHR following carvedilol. Larger portal vein diameter and lower serum sodium levels indicative of advanced portal hypertension and liver disease predict non-response to carvedilol.</p><p><b>PP-02-253</b></p><p><b>Enhancing Furosemide Bioavailability for the Management of Portal Hypertension Using Self Nano Emulsifying Drug Delivery</b></p><p><b>Pankajkumar Yadav</b></p><p><i>Shuats, Prayagraj, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> The obsjective of this study was to enhance the bioavailability of furosemide (FURO), an antihypertensive loop diuretic used in the management of portal hypertension, by improving its water solubility, permeability, and absorption after oral administration. To achieve this aim, a novel drug delivery system, Self Nano Emulsifying Drug Delivery System (SNEDDS), was employed.</p><p><b><i>Materials and methods:</i></b> Various oils, surfactants, and co-surfactants were tested to determine their ability to improve the solubility of FURO. The self-emulsification region was identified using pseudoternary diagrams, and SNEDDS formulations were developed accordingly. The formulations were characterized using zeta potential determination, droplet size analysis, dilution test, viscosity determination, in vitro dissolution studies, and in vivo pharmacodynamic evaluation.</p><p><b><i>Results:</i></b> Mean droplet size of the optimized formulation was found to be 26.8 nm. In vitro performance of the optimized preparation was satisfactory as observed by various analyses such as dilution test, emulsification time, and precipitation assessment. In vitro dissolution studies exhibited that the optimized SNEDDS formulation F3 exhibited a 1.7 fold increase in dissolution efficiency as compared to plain FURO and marketed formulations. In vivo studies showed enhanced bioavailability of F3 in terms of diuretic efficacy.</p><p><b><i>Conclusion:</i></b> The study confirms the potential use of SNEDDS formulation as an alternative to traditional oral formulations of FURO to enhance its bioavailability in the management of portal hypertension.</p><p><b>PP-02-254</b></p><p><b>Correlation of USS confirmed FLD and cirrhosis with VCTE in a cohort of Sri Lankans</b></p><p><b>Shiran Appuhamy</b>, Suranjan Viraj, Anjalika Madhubhashini, Sakshara Ranasinghe and Chamila Mettananda and Chamila Ranawaka</p><p><i>Colombo North Teaching Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Being a low-middle income country, Vibration-Controlled Transient Elastography (VCTE) (FibroScan) availability is very less in Sri Lanka and most rely on ultrasound scan (USS) for accessing fatty liver disease (FLD) and cirrhosis. Therefore we aim to study the correlation of USS and VCTE for FLD and cirrhosis in Sri Lankans.</p><p><b><i>Materials and Methods:</i></b> We conducted a descriptive study from October 2021 to August 2024 (34 months) at Gastroenterology and Hepatology department Colombo North Teaching Hospital Ragama. Patients with USS diagnosed fatty liver disease (FLD) or cirrhosis was referred for VCTE. Non-Significant fibrosis defined by liver stiffness measure (LSM) < 7.5 and significant fibrosis (SF) LSM 7.5 to 19 kPa and cirrhosis LSM > 19 kPa. No Steatosis defined as controlled attenuation parameter (CAP) <238 and steatosis CAP ≥238 dB/m.</p><p><b><i>Results:</i></b> Of the 1104 patients identified, 56% were male, mean age was 48. Patients with only FLD on USS was 657 (59.5%) and only cirrhosis was 447 (40.5%). Out of the 657 patients with FLD on USS, VCTE showed steatosis in 575 (87.5%). 82 (12.5%) had no steatosis. Out of 447 cirrhotics on USS, VCTE showed significant fibrosis in 191 (42.7%) and cirrhosis in 182 (40.7%). 74 (16.6%) had no significant fibrosis.</p><p><b><i>Conclusion:</i></b> USS showed to be good at identifying steatosis (specificity -87.5%). However USS had a false positive of 59.3% when identifying cirrhosis. VCTE is superior in identifying cirrhosis and quantifying fibrosis over USS. Therefor increasing VCTE availability will make a considerable impact on identifying patients with cirrhosis.</p><p><b>PP-02-255</b></p><p><b>Rates of Hepatic Fibrosis and Steatosis in a cohort of Sri Lankans referred for VCTE</b></p><p>Shiran Appuhamy, <b>Suranjan Viraj</b>, Anjalika Madhubhashini, Sakshara Ranasinghe, Chamila Mettananda and Chamila Ranawaka</p><p><i>Colombo North Teaching Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Data on rates of liver Fibrosis and Steatosis among Sri Lankan population is limited. Therefore, we aim to study the rates of hepatic fibrosis and steatosis measured by vibration-controlled transient elastography (VCTE) (FibroScan) in a cohort of Sri Lankans referred VCTE, due to specific indications.</p><p><b><i>Materials and Methods:</i></b> We conducted a descriptive study from October 2021 to August 2024 (34 months) at Gastroenterology and Hepatology department Colombo North Teaching Hospital Ragama. Patients referred for VCTE with indications of fatty liver disease(FLD) or cirrhosis on ultrasound scan (USS), obesity (BMI>30kg/m2) or type 2 diabetes were studied. All patients had VCTE. Significant fibrosis (SF) defined by liver stiffness measure (LSM) 7.5 to 19 kPa and Cirrhosis LSM> 19 kPa. Significant steatosis(SS) defined as controlled attenuation parameter (CAP) ≥290 dB/m.</p><p><b><i>Results:</i></b> Of the 1990 (100%) patients identified, 56% were male, mean age was 51 years. Indications for VCTE were USS with FLD 657 (33%), USS with cirrhosis 447 (22.5%), obesity 383 (19.2%) and diabeties 503 (25.3%). Rate of SF were 921 (46%) and cirrhosis 361 (18.1%). Rate of SS 1090 (54.8%). Obesity was present among 50.7% patients with SF, and 70.8% with SS. Diabetes was seen in 38.6% patients with SF and 53.9% with SS.</p><p><b><i>Conclusion:</i></b> Nearly half of the patients that underwent VCTE had Significant Fibrosis or Significant Steatosis, and 18% had cirrhosis. Obesity and diabetes was prevalent among patients with Significant Fibrosis or Significant Steatosis.</p><p><b>PP-02-256</b></p><p><b>Incidence and Mortality of Gallbladder Cancer in Hong Kong: a trend analysis</b></p><p><b>Junjie Huang</b>, Shui Hang Chow, Chenwen Zhong, Sze Chai Chan and Martin Wong</p><p><i>The Chinese University Of Hong Kong, Sha Tin, Hong Kong</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of gallbladder and extrahepatic bile duct cancer in Hong Kong.</p><p><b><i>Methods:</i></b> Data on new cases and deaths related to gallbladder and extrahepatic bile duct cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).</p><p><b><i>Results:</i></b> In 2020, a total of 445 new cases of gallbladder and extrahepatic bile duct cancer were reported, with an ASR of 2.3. The incidence was slightly higher among males (ASR=2.4) compared to females (ASR=2.1), and remarkably higher in the older population aged over 50 years (ASR=10.7) compared to the younger population (ASR=0.1). There were 151 newly reported deaths, with an ASR of 0.7. The mortality was marginally higher in males (ASR=0.8) than in females (ASR=0.6), and substantially higher in the older population (ASR=3.2) compared to the younger population (ASR=0.07). Trend analysis revealed a significant decreasing mortality trend for both sexes (AAPC: -3.7, 95% CI: -6.5, -0.9, p=0.02) and in the older population (AAPC: -4.3, 95% CI: -7.4, -1.1, p=0.02), while the incidence trends remained relatively stable over the study period.</p><p><b><i>Conclusion:</i></b> This population-based study demonstrated a stable incidence trend and a declining mortality trend for gallbladder and extrahepatic bile duct cancer in Hong Kong. Continued efforts on primary prevention through lifestyle modifications could be the next step to further reduce its incidence.</p><p><b>PP-02-257</b></p><p><b>Factors predicting advanced liver fibrosis in patients with Autoimmune hepatitis-Single Centered experience from Pakistan</b></p><p><b>Raja Taha Yaseen Khan</b>, Abbas Ali Tasneem and Nasir Hassan Luck</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To identify factors predicting advanced fibrosis in patients with autoimmune hepatitis (AIH) using non-invasive methods.</p><p><b><i>Material and Methods:</i></b> This cross-sectional study was conducted at the Hepatogastroenterology unit of Sindh Institute of Urology and Transplantation, Karachi and included the patients diagnosed with AIH based on the serology and histology. Advanced fibrosis and cirrhosis was defined using the Metavir score on liver biopsy. Multivariate logistic regression analysis was employed to identify predictors of advanced fibrosis.</p><p><b><i>Results:</i></b> The study included 162 AIH patients. Advanced fibrosis was observed in 70 (43.2%) patients. Multivariate analysis revealed that female gender (p<0.001) (OR 29.4, 95% CI 4.6-188.6), decreased TLC (p<0.001) (OR 0.2, 95% CI 0.087-0.468) and platelet count (p=0.001) (OR 1.034, 95% CI 1.015-1.054) and increased total bilirubin (p=0.003) (OR 165.8, 95% CI 5.7-485.1), INR (p<0.001) (OR 44.8, 95% CI 14.6-68.1),serum IgG (p=0.025) (OR 0.95, 95% CI 0.91-0.99) and ANA levels of >1:80 (p=0.027) (OR 2.3, 95% CI 1.11-4.66) were significantly associated with the presence of advanced fibrosis and cirrhosis on histology.</p><p><b><i>Conclusion:</i></b> This study identified several significant predictors of advanced fibrosis and cirrhosis in patients with autoimmune hepatitis (AIH). Female gender, decreased total leukocyte count (TLC) and platelet count, and raised serum IgG and ANA levels, international normalized ratio (INR) and total bilirubin were all independently associated with advanced fibrosis and cirrhosis. These findings emphasize the importance of these clinical and biochemical markers in identifying AIH patients at risk for advanced liver disease, aiding in early diagnosis and targeted management strategies.</p><p><b>PP-02-258</b></p><p><b>Clinical Profile of Wilson’s Disease Patients from Tertiary Care Centre of Coastal Karnataka</b></p><p><b>Abhay Mahajan</b>, Ganesh Bhat and Athish Shetty</p><p><i>Kmc, Manipal, Udupi, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The present study aimed to evaluate the clinical and biochemical characteristics of patients with Wilson's Disease.</p><p><b><i>Material & methods:</i></b> This retrospective study was conducted in the department of gastroenterology of tertiary care centre of coastal Karnataka from January 2021to January 2024. Patients with the confirmed diagnosis of Wilson disease irrespective of age and gender were included in the study. Patients’ information including demographic data, clinical and biochemical parameters were reported in a pre-designed format.</p><p><b><i>Results:</i></b> A total of 51 patients were included in the study, with a majority being male (30 patients, 58.8%). The mean age of onset was 11.21 ± 1.32 years, the mean delay in diagnosis was 13.12 ± 9.22 months, and the mean age at diagnosis was 15.13 ± 2.42 years. Among the patients, 15 (29.4%) were paediatric patient, and 36 (70.5%) were adults. Kayser-Fleischer rings (51%) were the most common initial presenting feature. Cirrhosis at diagnosis was present in 47.1% patients. On physical examination, 16 patients (45.7%) had hepatomegaly, and 19 (54.3%) reported splenomegaly. Neurological symptoms were present in 22 patients (43.1%), with poor scholastic performance being the most commonly reported neurological issue (13.6%). The majority of patients received zinc treatment (80.4%), followed by penicillamine (19.6%).</p><p><b><i>Conclusion:</i></b> This study adds to the existing data on Wilson's disease. In patients with no family history and no tell tale signs of liver cell failure,there might be a delay in diagnosis. Thus an early and guidelines based diagnosis can decrease morbidity and mortality of this treatable disease.</p><p><b>PP-02-259</b></p><p><b>Triglyceride-Glucose index role in liver fibrosis detection in patients with alcoholic liver disease</b></p><p><b>Dorina Osmanaj</b><sup>1</sup>, Xhensila Pemaj<sup>2</sup>, Adriana Babameto<sup>1</sup>, Marsela Sina<sup>1</sup>, Skerdi Prifti<sup>1</sup> and Arvit Llazani<sup>3</sup></p><p><sup>1</sup><i>Division of gastrohepatology, Mother Theresa University Hospital Center, Tirana, Albania;</i> <sup>2</sup><i>American Hospital Center, Tirana, Albania;</i> <sup>3</sup><i>Division of General Surgery, Mother Theresa University Hospital Center, Tirana, Albania</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Alcohol abuse can cause metabolic disorders such as increasing the resistance of insulin and alcoholic liver disease (ALD). The aim of this study is to evaluate the relation of Triglyceride-Glucose (TyG) index, liver fibrosis and Body Mass Index (BMI) in heavy drinking patients.</p><p><b><i>Material and Methods:</i></b> This is a retrospective study of 51 patients with alcohol addiction and ALD presented at our University Hospital Center from January-June 2023. Patients with diabetes were excluded from the study. Demographic, laboratory data, and BMI were collected for each patient. AST to Platelet Ratio Index (APRI) score and abdominal ultrasound were used to evaluate the stage of liver fibrosis. Patients were classified as overweight, normal weight and underweight according to World Health Organization.</p><p><b><i>Results:</i></b> All the patients were male with a mean age 45.63±10.61 and also heavy drinkers with 22 unit of alcohol consumption per day for 13 years. 49% of the patients had F2-F3 fibrosis, 35 % F3-F4 fibrosis, and 15% had F0-F1 fibrosis. 92% of patients had TyG index over 4.49 suggesting insulin resistance. No association was found between TyG index and stage of liver fibrosis (p=0.934). 65% of the patients were overweight 31% were normal weight and 4 % were underweight. No statistics significant association was found between TyG index and BMI (p=0.862).</p><p><b><i>Conclusions:</i></b> Although most of the patients who abuse with alcohol were over the cut off TyG index this was not correlated with their BMI or liver fibrosis staging.</p><p><b>PP-02-260</b></p><p><b>Fibrosis pattern in patients of MASLD with history of Diabetes mellitus and Dyslipidemia</b></p><p><b>Anuj Kumar Soni</b></p><p><i>Government Medical College, Thiruvananthapuram, Kerala, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aim:</i></b> Metabolic Dysfunction-associated Steatotic Liver Disease(MASLD) has emerged as a major health problem globally. We tried to find the pattern of fibrosis in different groups of MASLD patients with history of (h/o) diabetes mellitus(DM) and dyslipidemia(DLP).</p><p><b><i>Methods:</i></b> Cross-sectional observational study of 2240 MASLD patients. Liver fibrosis was assessed using Vibration controlled transient elastography (VCTE). INASL 2022 criteria was applied to define significant fibrosis. One way ANOVA test was applied to compare means in the more than two groups followed by Post Hoc test (Tukey HSD). Chi square test was applied to calculate association of fibrosis with DM and DLP.</p><p><b><i>Results:</i></b> 334(14.9%) patients had h/o DM, 460(20.5%) patients had h/o DLP. 177(7.9%) patients had h/o both DM and DLP. Mean VCTE in group of patients having h/o both DM & DLP was 10.33±4.86 kpa and 57.1% patients had significant fibrosis in this group. Mean VCTE was 10.76±5.84 kpa in the DM alone group and 9.01±3.61 kpa in the DLP alone group (p<0.05). 58.0% had significant fibrosis among the patients with h/o DM alone and 45.9% in the DLP alone group (p<0.05).</p><p><b><i>Conclusion:</i></b> History of DM is a greater driver of fibrosis than DLP in patients with MASLD. Adding on DLP to DM does not add any significant difference in development of fibrosis in MASLD.</p><p><b><i>Keywords:</i></b> MASLD, DM, DLP, VCTE</p><p><b>PP-02-261</b></p><p><b>Real-World Analysis: The Effect of Recombinant Human Thrombopoietin on Chronic Liver Disease-Thrombocytopenia with Concurrent Infections</b></p><p><b>Xun Wei</b> and Yi Kang</p><p><i>Henan Provincial People's Hospital, Zhengzhuo, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to assess the therapeutic effect of recombinant human thrombopoietin (rhTPO), on chronic liver disease (CLD) -related thrombocytopenia (TP) with concurrent infections.</p><p><b><i>Materials and methods:</i></b> We retrospectively analyzed the clinical data from 259 patients diagnosed with CLD and TP who underwent treatment with rhTPO at the Department of Infectious Diseases, Henan Provincial People's Hospital, from January 2021 to October 2023. And we evaluated changes in platelet count (PLT) and monitored treatment-related adverse events, including bleeding and thrombus formation.</p><p><b><i>Results:</i></b> rhTPO significantly increased platelet counts from day 3, effective in both infected and non-infected patients. Patients with Child-Pugh grades A and B demonstrated a more substantial increase in PLT post-treatment compared to those with Child-Pugh grade C. By day 7, significant differences in PLT increments were evident among the three Child-Pugh groups, with respective values of 38.0 × 10^9/L, 32.0 × 10^9/L, and 13.0 × 10^9/L (P = 0.003). The therapeutic impact of rhTPO monotherapy versus its combination with avatrombopag varied, with the former showing a quicker response time compared to the latter (P = 0.011). No serious adverse events were reported.</p><p><b><i>Conclusion:</i></b> rhTPO is effective in managing CLD-related TP, reducing bleeding risks, and transfusion reliance, potentially improving patient prognosis.</p><p><b>PP-02-262</b></p><p><b>Surgical management of Pancreatic Insulinoma: Case report of a rare tumor</b></p><p><b>Nirodha Abeywardhana</b>, Bandula Samarasinghe, Kasun Herath, Imila Jayakodi and Sathira Premarathna</p><p><i>Teaching Hospital Peradeniya, Kandy, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Insulinoma is a rare neuroendocrine tumor that secretes insulin inappropriately causing hypoglycemic episodes. Patients typically present with autonomic and neuroglycopaenic symptoms. Elevated C peptide levels confirms the diagnosis of insulinoma. Preoperative localization of the tumor is important. Here we present a case report of a young lady who was referred from medical clinic with recurrent hypoglycaemic episodes over 2 years. Insulinoma was localized using contrast enhanced computed tomography. Patient was completely free of symptoms after the surgery.</p><p><b><i>Case Description:</i></b> We report a case of 25 year old lady who was investigated for recurrent fainting attacks over 2 years. She also had on and off seizures as well. She also has had Faintishnes and dizziness which improves following meals. Her serial random blood sugar levels were between 60 mg/dl -70 mg/dl and there were episodes of below 50mg/dl as well. Contrast enhanced computed tomography was done and it showed features of a insulinoma of the distal pancreas. Laparotomy and excision of insulinoma done and following that the symptoms improved completely. Her post-operative period was uneventful.</p><p><b><i>Discussion:</i></b> Insulinoma is a very rare tumor. Clinicians should have a high index of suspicion for this condition in patients with history of recurrent episodes of faintishness or seizures. Proper patient follow-up and repeated analysis helps to recognize early and refer for definitive treatment.</p><p><b>PP-02-263</b></p><p><b>A serial case of melanoma maligna rectum: should we reconsider abdominoperineal resection for treatment?</b></p><p><b>Kezia Christy</b> and Alma Wijaya</p><p><i>Padjajaran Univeristy, Bandung, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Melanoma maligna of rectum is an extremely rare disease and poor prognosis with surgical treatment controversy. We present two cases of a 61-y.o- female and 56-y.o- male with melanoma maligna of rectum who underwent APR.</p><p><b><i>Case reports:</i></b> A 61-year-old female with a history of rectal bleeding since 2 months ago. Colonoscopy biopsy showed melanoma maligna at distal rectum. CT Scan showed intrahepatic metastasis. The patient underwent APR and died two weeks after APR.</p><p>A 56-year-old male with anal pain since 2 months ago. CT Scan showed intrahepatic and pulmonary metastasis. The patient underwent APR and pathological anatomy showed melanoma maligna at rectum. The patient died one month after the operation.</p><p><b><i>Discussion:</i></b> The surgical treatment is still controversy. The 5-year survival rate can range from 16 to 34%. In patients who have metastasis at the time of diagnosis, the disease-free survival rate may drop to 16% from 22%. The controversy has been whether APR is needed or wide local excision (WLE) is adequate for complete treatment. APR has a highly morbid operation,but thought to be the best to control anorectal melanoma. WLE are quicker recovery, no need for a stoma, and minimal impact on bowel function. Chemotherapy, radiation therapy, and immune therapy have a limited role.</p><p><b><i>Conclusion:</i></b> The rarity of this disease and the limited number of patients who present with early disease, have prevented definitive trials examining the optimal treatment of curable anal melanoma. These 2 cases showed poor result of APR to treat melanoma maligna of rectum.</p><p><b>PP-02-264</b></p><p><b>Feasibility and outcomes following implementation of modified-ERAS for laparoscopic colorectal cancer surgery in Sri Lanka</b></p><p><b>Umesh Jayarajah</b><sup>3</sup>, Chamila Lakmal<sup>3</sup>, Nishenth Muralidharan<sup>3</sup>, Tharusha Madushan<sup>3</sup>, Nilushika Perera<sup>2</sup> and Bawantha Gamage<sup>2</sup></p><p><sup>1</sup><i>Department of Surgery, National Hospital of Sri Lanka, COLOMBO 4, Sri Lanka;</i> <sup>2</sup><i>Department of Surgery, Faculty of Medical Sciences, University of Sri Jayewardenepura, Colombo, Sri Lanka;</i> <sup>3</sup><i>Department of Surgery, Colombo South Teaching Hospital, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Enhanced Recovery after Surgery (ERAS) protocols have improved patient outcomes. ERAS with some modifications (mERAS) has been introduced at a resource limited tertiary care centre in Sri Lanka. This study explores the feasibility and potential benefits of mERAS for laparoscopic colorectal procedures in the local context.</p><p><b><i>Materials and Methods:</i></b> A prospective study was conducted including diagnosed patients with colorectal carcinoma who underwent laparoscopic surgical management with modified ERAS protocol (n=237, males= 58%, median age: 65 years). A multidisciplinary change in the routine practice was implemented based on mERAS. A descriptive analysis was performed with respect to clinical and treatment characteristics.</p><p><b><i>Results:</i></b> Almost all adhered to pre-op mERAS (99%) protocol. Most underwent anterior resection (n=133,56.1%). Most had a pre-operative albumin of >35g/Litre(84%). Some required conversion to open due to technical difficulties (n=27,11.3%). The majority were mobilized on day-1 (65.2%) and day-2 (27.3%) and nasogastric tube removed on day-1(73.3%). Most were discharged from ICU care after post-op day-1 (40%) or day-2 (39%). Abdominal drain was removed by day-4 in 87.8%. Solids were started by day-3 in 85.4%. Post–op complication rates (n=37,15.6%) were within predicted value and most were surgical site infections (n=15) and anastomotic leak was seen in 6 patients. Median hospital stay was 5 days (Range: 4-12).</p><p><b><i>Conclusion:</i></b> Modified ERAS was practical and effective in our unit along with complication rates and recovery rates comparable with Western values where the standard ERAS is practiced. Thus, we recommend the modified ERAS protocol for resource limited settings.</p><p><b>PP-02-265</b></p><p><b>Incidence and pattern of oesophageal cancer in Sri Lanka from 2001-2019</b></p><p><b>Umesh Jayarajah</b>, Jesuthasan Mithushan and Sanjeewa Seneviratne</p><p><i>Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> An increase in the global incidence of oesophageal cancer has been noted in the last few decades. This study was aimed to describe the trends in the incidence of oesophageal cancer in Sri Lanka which would enable the planning of oesophageal cancer care.</p><p><b><i>Materials and methods:</i></b> The trends in the incidence of oesophageal cancer were calculated using the published data from the Sri Lanka Cancer Registry for the period from 2001 to 2019. The trends in the WHO age standardised incidence rates were presented using the Joinpoint regression analysis.</p><p><b><i>Results:</i></b> From 2001 to 2019, 26,459 oesophageal cancers were diagnosed, with a male predominance of 53%. The overall mean age was 62.8 years (males=62.2 and females=63.5). The highest incidence was seen in the 70–74 age group. The WHO age-standardised incidence of oesophageal cancer has risen significantly from 5.78 to 8.46 per 100,000 from 2001 to 2019. This increase was prominent till 2016 and thereafter showed a decreasing trend till 2019. The magnitude of the rise in incidence was higher for males [estimated annual percentage change (EAPC):4.6 with a steady trend, p<0.05] compared with females, who had a variable trend with a non-significant EAPC.</p><p><b><i>Conclusion:</i></b> A statistically significant increase in the incidence of oesophageal cancer in Sri Lanka was noted from 2001 to 2019, with greater significant proportional increases in men. Further studies, including tumour stage, underlying reasons, and mortality, may help better understand changing patterns of disease burden. Given the rising trend, community-based screening policies should be considered.</p><p><b>PP-02-266</b></p><p><b>Incidence and pattern of gastric cancer in Sri Lanka from 2001-2019</b></p><p><b>Umesh Jayarajah</b>, Jesuthasan Mithushan and Sanjeewa Seneviratne</p><p><i>Department of Surgery, Faculty of Medicine, University of Colombo, COLOMBO, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Varying patterns in the incidence of gastric cancer (GC) has been reported globally. This study aims to describe the trends in the incidence of gastric cancer in Sri Lanka.</p><p><b><i>Materials and methods:</i></b> The trends in the incidence of gastric cancer were calculated using the published data from the Sri Lanka Cancer Registry for the period from 2001 to 2019. The trends in the WHO age standardised incidence rates were presented using the Joinpoint regression analysis.</p><p><b><i>Results:</i></b> From 2001 to 2019, 7914 gastric cancers were diagnosed, with a male preponderance of 71%. The overall mean age was 60.7 years, which was similar for males and females. The incidence of gastric cancer was highest in the 70-74 year age group. The WHO age-standardised incidence of gastric cancer in Sri Lanka has increased from 1.06 to 3.3 per 100,000 from 2001–2016, followed by a decreasing trend to 2.9 per 100,000 in 2019. During the rising trend, the estimated annual percentage change (EAPC) was 8.3 (p<0.05). Females showed a higher proportional increase in incidence (EAPC: 9.9 vs. 8.2).</p><p><b><i>Conclusion:</i></b> A rising incidence of gastric cancers is likely due to the combination of better reporting and a true increase in incidence. A greater and more significant proportional increase in incidence was noted in females. Future studies analysing tumour characteristics and mortality would enable a better understanding of the burden of gastric cancer and potential underlying causes for the increasing incidence.</p><p><b>PP-02-267</b></p><p><b>Chyle Leak: A Dreaded Surgical Complication Following Abdominal Surgeries</b></p><p><b>Jayamini Kaushalya</b> and Anjana Abayasinghe and Samitha Senevirathne and Duminda Subasinghe</p><p><i>Professorial Surgical Unit, National Hospital-Colombo Sr Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Chyle leak is a rare but severe complication of abdominal surgeries, leading to high morbidity and mortality due to associated metabolic disturbances, immunosuppression, poor wound healing, and wound-related issues. It is typically suspected during the postoperative period when drain output appears milk-like with elevated volumes. Diagnosis is confirmed biochemically by measuring triglyceride levels in the drainage fluid, which should exceed 100 mg/dL or be higher than serum levels.</p><p><b><i>Case Report:</i></b> A 62-year-old female who was investigated for anemia was diagnosed with multiple gastrointestinal stromal tumors (GISTs) originating from the greater curvature of the stomach and fundus, with one large tumor adhering to the pancreas and the transverse colon. After an initially uncomplicated postoperative period, she developed an increase in abdominal drain volume and a change in fluid color to light yellow by postoperative day six. Analysis of the drain fluid revealed elevated triglyceride levels compared to serum and normal amylase levels, confirming a chyle leak. The condition was managed effectively with dietary modifications and pharmacological therapy, avoiding the need for invasive procedures.</p><p><b><i>Discussion:</i></b> Prompt diagnosis and conservative management are essential for addressing chyle leaks. Initial treatment typically involves low fat diet, pharmacological options like somatostatin analogues and pancreatic lipase inhibitors, and measures to reduce intra-abdominal pressure, including limiting physical activity and using antitussives and laxatives. Surgical intervention is considered only for refractory leaks or severe metabolic disturbances. This case demonstrates the success of conservative management strategies in resolving a chyle leak without invasive procedures.</p><p><b>PP-02-268</b></p><p><b>Identifying prognostic factors and independent risk factors in colorectal cancer</b></p><p>Pasindu Nanayakkara<sup>1</sup>, <b>Ruseik Rahumath</b><sup>1</sup>, Dileepa Ediriweera<sup>2</sup>, Gayana Mahendra<sup>3</sup>, Janaki Hewavisenthi<sup>3</sup>, Sumudu Kumarage<sup>4</sup> and Pramodh Chandrasinghe<sup>4</sup></p><p><sup>1</sup><i>Postgraduate Institute of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>Health Data Science Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, Faculty of Medicine, University of Kelaniya, Sri Lanka;</i> <sup>4</sup><i>Department of Surgery, Faculty of Medicine, University of Kelaniya, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Establishing factors influencing the overall survival is crucial for improving prognostication and treatment in colorectal cancer(CRC). This study aims to identify key prognostic factors and independent risk factors in CRC by analyzing survival outcomes.</p><p><b><i>Materials and Methods:</i></b> In CRC patients undergoing surgical resection from January-2013 to May-2019, followed until May-2024, Age, Sex, tumor differentiation(TD), T stage, N stage, having a positive LN (PLN) & LVI along with time since surgery to last follow-up/death were collected. Survival rates were estimated using Kaplan-Meier curves and the Log-rank test(LRT) the level of significance being p<0.05. Cox proportional-hazard models identified survival-associated features, with forward variable selection determined by the log-likelihood ratio test.</p><p><b><i>Results:</i></b> Of 137 participants, 54(39.42%) were males, with a median age of 61(IQR: 52-68). 34 out of 58 deaths were caused by CRC. The overall 5-year-survival(OS) rate was 60.4%(95%CI, 52.5%-69.5%), and the corresponding disease-specific survival(DSS) rate was 72.2%(95%CI, 64.5%-80.8%). When considering OS, the following factors indicated a significant difference in the LRT: LVI (P < 0.0001), N0-N1-N2 stage (P=0.022, P=0.051), PLN (P = 0.0026, TD(P=0.0003), T4 Stage(P=0.047).</p><p>Results of forward selection showed the model with LVI, age, and N stage was the best to explain OS. The hazard ratios were: LVI =3.094(95% CI,1.782-5.372, P < 0.0001), age =1.029(95% CI,1.005-1.054, P=0.0173), and N stage =1.545(95% CI,1.062-2.247, P=0.0229).</p><p><b><i>Conclusion:</i></b> For survival, age, tumor differentiation, T stage, N stage, and LVI were significant factors. LVI, age, and N stage were independent prognostic factors, with LVI being the strongest predictor.</p><p><b>PP-02-269</b></p><p><b>Prognostic significance of categorizing lymphovascular invasion as nodal stage n1 in colorectal cancer</b></p><p><b>Pasindu Nanayakkara</b><sup>1</sup>, Ruseik Rahumath<sup>1</sup>, Dileepa Ediriweera<sup>2</sup>, Gayana Mahendra<sup>3</sup>, Janaki Hewavisenthi<sup>3</sup>, Sumudu Kumarage<sup>4</sup> and Pramodh Chandrasinghe<sup>4</sup></p><p><sup>1</sup><i>Postgraduate Institute of Medicine, University of Colombo, Sri Lanka;</i> <sup>2</sup><i>Health Data Science Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, Faculty of Medicine, University of Kelaniya, Sri Lanka;</i> <sup>4</sup><i>Department of Surgery, Faculty of Medicine, University of Kelaniya, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Positive nodal metastases indicate lymphovascular invasion(LVI) in colorectal carcinoma(CRC) is a known concept. The reverse situation has not been investigated. This study aims to analyze the prognostic significance of categorizing LVI-positive nodal negative cases as stage N1.</p><p><b><i>Materials and Methods:</i></b> The nodal stage, LVI status, and time since surgery to the last follow-up/death were obtained from the database for patients who underwent surgical resection from January-2013 to May-2019, with follow-up until May-2024. Cox proportional-hazard models were used to identify features associated with survival. LVI-positive, N0 cases were transferred to N1 stage, establishing a new model(NM) for statistical analysis for comparison with the existing model prior to transfer -Traditional model(TM).</p><p><b><i>Results:</i></b> Of 137 participants, 41(29.93%) were LVI positive. Nodal stages were N0-65, N1-51, N2-21. Of the 96 LVI negative cases, 53 were in N0 stage and 43 were nodal positive. Among 41 LVI positive cases, 29had nodal metastasis, while 12 did not. These 12 cases were transferred to N1. Hazard ratio for overall survival (HR) for LVI in NM was 3.094(95%CI,1.782-5.372,P<0.0001) compared to 2.706(95%CI,1.537-4.764,P=0.0006) in TM. HRs for TM and the NM were 1.545(95%CI,1.062-2.247,P=0.0229) and 1.760(95%CI,1.169-2.648,P=0.0068), respectively.</p><p>Transferring LVI positives changed the N stage coefficient P-value from 0.0229 to 0.006753 (Wald test) and increased concordance from 0.725 to 0.726 in NM.</p><p><b><i>Conclusion:</i></b> The NM demonstrate superior model evaluation parameters than TM. Transferring LVI positives from N0 to N1 improved prognosis accuracy, suggesting need to explore stage migration from N0 to N1 with positive-LVI without nodal metastases in a larger cohort.</p><p><b>PP-02-270</b></p><p><b>Clinicopathological Characteristics and Lymph Node Metastasis Rates in Early Gastric Lymphoepithelioma-like Carcinoma</b></p><p>Tae-Se Kim, <b>Youngeun Oh</b>, Ji Yeong An, Yang Won Min, Hyuk Lee, Byung-Hoon Min, Jun Haeng Lee, Poong-Lyul Rhee and Jae J. Kim</p><p><i>Samsung Medical Center, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Lymphoepithelioma-like carcinoma (LELC) is a rare subtype of gastric cancer. We aimed to identify the clinicopathological features and rate of lymph node metastases (LNM) to investigate the feasibility of endoscopic submucosal dissection (ESD) for early gastric LELC confined to the mucosa or submucosa.</p><p><b><i>Material and Methods:</i></b> We compared the clinicopathological characteristics of 116 early gastric LELC patients and 5,753 early gastric well- or moderately differentiated (WD or MD) tubular adenocarcinoma patients treated by gastrectomy.</p><p><b><i>Results:</i></b> Compared to WD or MD early gastric cancer (EGC) patients, early LELC patients were younger and had a higher prevalence of proximally located tumors. Despite more frequent deep submucosal invasion (86.2% versus 29.8%), lymphatic invasion was less frequent (6.0% versus 16.2%) in early LELC patients than in WD or MD EGC patients. Among tumors with deep submucosal invasion, tumor size was smaller, lymphatic invasion was less frequent (6.0% versus 40.2%) and the rate of LNM was lower (10.0% versus 19.4%) in patients with LELC than those with WD or MD EGC. Overall rate of LNM in early LELC patients was 8.6% (10/116). Risk of LNM in patients with mucosal, shallow submucosal invasive, or deep submucosal invasive LELC was 0% (0/6), 0% (0/10), and 10% (10/100), respectively.</p><p><b><i>Conclusion:</i></b> Early LELC is a distinct subtype of EGC with more frequent deep submucosal invasion but less lymphatic invasion and LNM than WD or MD EGCs. ESD may be considered curative for patients with early LELC confined to mucosa or shallow submucosa, given its negligible rate of LNM.</p><p><b>PP-02-271</b></p><p><b>Aberrant right hepatic artery (ARHA) with dominant supply during pancreaticoduodenectomy: We report you decide</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Nimeshana Bandara<sup>2</sup> and Sivasuriya Sivaganesh<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Aberrant right hepatic arteries (ARHA) occur in 15-20% of the population. Most arise from the superior mesenteric artery (SMA) and pass posterior to the head of the pancreas (HoP) and less commonly through or anterior to the HoP. Aberrant RHAs may be accessory or replaced (rARHA).</p><p><b><i>Case description:</i></b> A 61-year-old male with a bleeding duodenal malignancy prepared for a pancreaticoduodenectomy had a replaced RHA passing posterior to the HoP on CT. This was confirmed at surgery with early isolation and preservation. Postoperative recovery was uneventful, and histopathology confirmed an R0 resection. The key challenges when operating with ARHAs are preservation of arterial flow to the right lobe of the liver and biliary tract and obtaining R0 resection. Meticulous preoperative CT imaging and evaluation of the vasculature by the surgeon himself, as well understanding of surgical anatomy and surgical planning are crucial to achieving the above outcomes.</p><p><b><i>Discussion:</i></b> Identification and preservation of an rARHA is mandatory during pancreatico-biliary resections. Failure could lead to ischaemia of the right hepatic lobe and biliary tree with early bilio-enteric anastomotic breakdown or late strictures. Pre-operative evaluation of arterial anatomy and planning surgical strategy prevents inadvertent injury. A ventral instead of conventional dorsal approach, early right to left retro-pancreatic exposure of the SMA are advocated. There are 4 options to manage ARHA. Preservation, Transection and reconstruction, Sacrifice (presence of s dominant RHA) and Pre-operative embolization to facilitate collateral formation.</p><p><b>PP-02-272</b></p><p><b>Forgotten remedy for duodenal tumours: Surgical ampullectomy in the era of interventional endoscopy</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Nimeshana Bandara<sup>2</sup>, Nilesh Fernandopulle<sup>1,2</sup> and Sivasuriya Sivaganesh<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Ampullary or duodenal adenomas are uncommon digestive tract tumours. Conventional treatment with surgical ampullectomy and pancreaticoduodenectomy has been largely superseded by endoscopic resection. However, large, broad-based tumours still require surgical excision.</p><p><b><i>Case description:</i></b> Two females aged 28 (patient 1) and 61 years (patient 2) investigated for dyspeptic symptoms had large polyps in the 2nd to 3rd parts of the duodenum. Endoscopic resection was not done due to their size and broad-based attachment. P1 had an open transduodenal ampullectomy and P2 a local papillectomy. Access was via right subcostal and midline laparotomies followed by Kocherisation and a longitudinal duodenotomy. Submucosal saline injection was performed to facilitate mucosal excision.</p><p>P1 had a tumour arising from the ampulla of Vater enclosing the common pancreatico-biliary channel. She underwent an ampullectomy and a papilloplasty. P2 had an anterior wall polyp of the 2nd and 3rd part of the duodenum and underwent a polypectomy A full thickness defect resulting from excision was primarily closed. The duodenotomies were closed in 2 layers. Both had uneventful post-operative periods. Both patients had tubular adenoma with low grade dysplasia.</p><p><b><i>Discussion:</i></b> Open transduodenal excision is a safe and effective therapeutic option for large, benign duodenal polyps not amenable to endoscopic resection. Caution should be exercised for lesions arising in the pancreatico-mesenteric aspect to avoid challenges with closure.</p><p><b>PP-02-273</b></p><p><b>Atypical Hepatic Metastasis of Pancreatic Adenocarcinoma Unveiled Through Persistent and Unresolved Pyrexia</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Chamod Nirmal<sup>2</sup>, Umesh Jayarajah<sup>2</sup>, Harshima Wijesinghe<sup>3</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>Department of Pathology, University of Colombo, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The diagnosis of neoplastic fever poses a considerable diagnostic challenge due to the necessity of excluding identifiable etiologies. Fever as a predominant clinical manifestation of pancreatic adenocarcinoma is an uncommon occurrence, with limited instances documented in the medical literature. Herein, we present an atypical case of metastatic pancreatic carcinoma primarily characterized by pyrexia of unknown origin (PUO).</p><p><b><i>Case Description:</i></b> A 63-year-old male of Sri Lankan origin, a non-smoker with a medical history notable for diabetes, hypertension, and dyslipidemia, presented with a two-month history of fever, anorexia, and weight loss. Despite completing treatment for positive serology for paratyphi, his symptoms persisted, and inflammatory markers remained elevated, while all other infectious screening tests returned negative results. Subsequent evaluation revealed hypodense lesions in the distal pancreas and multiple liver lesions with ring enhancement on imaging studies. Histopathological examination obtained via CT-guided biopsy confirmed the diagnosis of pancreatic adenocarcinoma with liver metastasis. Despite receiving symptomatic management for fever and palliative care, the patient ultimately succumbed to the disease.</p><p><b><i>Discussion:</i></b> In cases where atypical liver metastases are observed with evidence of ring enhancement on computed tomography (CT) imaging, biopsy becomes imperative for accurate diagnosis and subsequent treatment planning. Hence, in the setting of atypical metastatic pancreatic adenocarcinoma, the recommended approach entails palliative chemotherapy alongside pharmacological management of fever symptoms.</p><p><b>PP-02-274</b></p><p><b>Association between intestinal fibrosis and clinical recurrence after ileocolonic resection in stenotic Crohn's disease patients</b></p><p><b>Tianyi Che</b>, Jiaxin Wang, Yao Zhang, Yubei Gu and Duowu Zou</p><p><i>Department of Gastroenterology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aim to investigate the association between fibrosis at the site of intestinal stenosis and clinical recurrence after ileocolonic resection in patients with stenotic Crohn's disease.</p><p><b><i>Materials and Methods:</i></b> A retrospective, single-center study was conducted on stenotic Crohn's disease patients who received ileocolonic resection between January 2020 and December 2022. The most representative pathological slides from the resected stenotic intestinal tissues were selected. We used Masson's trichrome staining to visualize collagen fibers and calculated the proportion of collagen fiber area in the slides using the Image-Pro-Plus software to assess the degree of intestinal fibrosis. Baseline information and postoperative clinical recurrence were collected through electronic medical record systems and telephone follow-up. Survival analysis and Cox regression models were used to assess the impact of intestinal fibrosis on clinical recurrence.</p><p><b><i>Results:</i></b> Sixty-nine patients were included in this study. 29 (42%) of the patients experienced clinical recurrence during the follow-up period. Medium (HR 16.545, 95%CI 2.164-126.464, P=0.007) and high (HR 32.997, 95%CI 4.304-252.986, P=0.001) degree of intestinal fibrosis were associated with clinical recurrence after surgery in patients with stenotic Crohn's disease. This association remained significant when considering fibrosis only in the mucosa, submucosa, or muscular layers.</p><p><b><i>Conclusion:</i></b> The degree of fibrosis at the site of intestinal stenosis was significantly associated with clinical recurrence after ileocolonic resection in patients with stenotic Crohn's disease. Intestinal fibrosis should be considered in postoperative treatment and follow-up decision-making.</p><p><b>PP-02-275</b></p><p><b>Recurrence after ileo-caecal resection in Crohn’s disease – Lower with post-operative colonoscopy within 12 months</b></p><p>Millicent DeVries and <b>Rajan Patel</b></p><p><i>Christchurch Hospital, Christchurch, New Zealand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ileo-caecal (IC) resection for patients with small bowel Crohn’s disease is common. Complications of disease recurrence at the anastomosis can be prevented with optimised post-operative management. We aimed to investigate the incidence of disease recurrence following IC resection and identify associated factors.</p><p><b><i>Materials and Methods:</i></b> Retrospective review of the most recent 50 patients who underwent IC resection for Crohn’s disease with at least 12 months follow up. Data obtained from Business Intelligence Data & Analytics from 2018-2023. Electronic records reviewed for demographics, post-operative colonoscopy findings and medications initiated post-surgery. Statistical analysis performed using Fishers Exact test.</p><p><b><i>Results:</i></b> Cohort comprised 50 patients with median age 39 years (range 16-76); 42% female. Mean follow-up duration from surgery 1268 days (range 401 - 2085). Seventy-four percent of resections were laparoscopic, and 88% received prophylactic metronidazole. Post operative thiopurine or biologic initiated in 44% of patients (18% received thiopurine only, 82% biologic +/- thiopurine). Post-operative colonoscopy performed in 62% of patients, with mean time to endoscopy 19 months (range 6-55 months); 22% had colonoscopy within 12 months.</p><p>Among 44 patients assessed endoscopically or radiologically, 45.6% showed anastomotic recurrence. Recurrence did not differ based on post-operative metronidazole use (p=0.5). However, patients who underwent colonoscopy within 12 months of surgery had a significantly lower recurrence rate (p=0.04). No repeat resections occurred during the follow-up period.</p><p><b><i>Conclusion:</i></b> Post-operative metronidazole is not associated with reduced recurrence rates in the biologic era. Post-operative colonoscopy, and not radiological assessment, within the first year of surgery correlates with disease remission.</p><p><b>PP-02-276</b></p><p><b>Clinico-pathological characteristics of patients with malignant liver lesions: preliminary results from an HPB surgical unit</b></p><p><b>Duminda Subasinghe</b><sup>1</sup>, Ashan Jayawickrama<sup>3</sup>, Nilesh Fernandopulle<sup>1</sup>, Vihara Dassanayake<sup>2</sup> and Sivasuriya Sivaganesh<sup>1</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>Department of Anaesthesiology and Critical care, University of Colombo, Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Liver malignancies are the fourth commonest cause of cancer-related deaths globally. Of these, secondary liver malignancies are more common, especially colorectal metastases. This study studies the demography, clinicopathological features and management of malignant liver lesions (MLL) in a tertiary hepatobiliary center.</p><p><b><i>Materials and Methods:</i></b> A prospective study was conducted on patients with MLL (n=44) presenting from May 2023 – May 2024. Data on clinicopathological and radiological features were obtained from a custom-made database, followed by a descriptive analysis.</p><p><b><i>Results:</i></b> The majority were males (84%) with a mean age of 63.9 (38-82) years. Most patients (47.7%) were from the Colombo district. Constitutional symptoms were common (59%). Alcohol (n=25,56.8%) and diabetes (n=24,54.5%) were major associations, with (n=11,25%) diagnosed with chronic liver disease. Most lesions on CT were multifocal (n= 23, 52.2%), followed by solitary lesions (n= 17,38.6%). Primary malignancies included HCCs (n=19,43.1%), intrahepatic-cholangiocarcinomas (n=3,6.8%) and combined HCC/Cholangiocarcinoma (n=2,4.54%). Metastases were mainly from the pancreas (n=6,13.6%), large bowel (n=5,11.3%) and distal CBD (n=4,4.54%). Management included palliation (n= 20,45.4%), hepatectomy (n=13,29.5%), TACE (n=6,13.6%), and ablative therapy (n= 3, 6.8%). Surgery included right hepatectomy (n=4, 30.7%), left lateral sectionectomy (n=4,30.7%), non-anatomical resection (n=2,15.3%), segmentectomy (n=2,15.3%) and parenchymal sparing hepatectomy (n=1,7.69%).</p><p><b><i>Conclusion:</i></b> The clinical and demographic spectrum of malignant liver tumors observed in this cohort are similar to that in Asia. However, liver secondaries in Asia predominantly originates from lung and colorectal primaries compared to pancreatic and colorectal origins in our cohort, which affects survival rates.</p><p><b>PP-02-277</b></p><p><b>Successful management for necrotizing fasciitis due to colon cancer with intracorporeal jejunocolic bypass</b></p><p><b>Rio Miyashita</b> and Tadayoshi Yamaura and Yuki Tokuyama and Masasaaki Moriyama and Makoto Kawase and Satoshi Kanto and Yosuke Kinjo and Nobukazu Kuroda</p><p><i>National Hospital organization Himeji Medical Center, 68, Honmachi, Himeji, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Necrotizing fasciitis (NF) is a severe infectious disease. NF derived from colon cancer perforation is rare. The treatment for this type of NF needs balancing cancer and infection treatment. Nevertheless, keeping this balance is difficult, especially in the elderly.</p><p><b><i>Case report:</i></b> The patient is a 90–year–old female with dementia. The abdominal wall bulge had appeared from 2 weeks ago. Abdominal CT scan and colonoscopy revealed ascending colon cancer that invaded the abdominal wall and right external iliac artery. Additionally, colon perforation causes NF mainly in right side abdominal wall, spreading beyond the midline on ventral side, to the left side on dorsal side. As initial therapy, she was treated with antibiotic administration, and not extensive surgical debridement because of her age. After 4 weeks of this, her condition became better. However, she needed the prevention of colonic juice to flow into an abdominal wall for oral intake and infection control. Extended resection of cancer was hard for her. Stoma management was also hard due to her dementia. Finally, we performed laparoscopic intracorporeal jejunocolic bypass with all trocars placed the left side of abdominal wall: normal healthy side. On 24 postoperative days, she was discharged without any complications including surgical site infection.</p><p><b><i>Discussion:</i></b> The strategy for NF due to unresectable cancer has no absolute answer. We believe that less invasive treatment for this severe and poor prognosis disease improves their quality of life. We present successful management for NF owing to colon cancer in an extremely old patient.</p><p><b>PP-02-278</b></p><p><b>Caterpillar hump of right hepatic artery at laparoscopic cholecystectomy: Picture Story based on case report</b></p><p><b>Duminda Subasinghe</b><sup>1,2</sup>, Nimeshana Bandara<sup>2</sup> and Sivasuriya Sivaganesh<sup>1,2</sup></p><p><sup>1</sup><i>Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Anomalies in and around the hepatocystic triangle are common (20-50%) with variations in the origin, course and branches of the RHA documented. A sinuous, tortuous RHA coursing through the hepatocystic triangle is a less common but potentially dangerous anomaly. This morphology of the RHA is referred to as the caterpillar or Moynihan’s hump.</p><p><b><i>Case Description:</i></b> A 26-year-old female presenting with biliary pain and elevated bilirubin levels was found to have choledocholithiasis. She underwent endoscopic retrograde cholangiopancreatography (ERCP) and ductal clearance followed by a laparoscopic cholecystectomy 6 weeks later. During the dissection of the Calot’s triangle, a prominent tortuous artery was observed passing anterior to a short cystic duct. This was identified as a single loop caterpillar or Moynihan’s hump of the right hepatic artery (RHA) from which a short cystic artery originated (Figure 1,2). Cholecystectomy was completed after the demonstration of the critical view of safety and division of the cystic artery and duct between clips.</p><p><b><i>Discussion:</i></b> The incidence of the RHA caterpillar hump is 7%, with 40% passing anterior and 60% passing posterior to the cystic duct. Single and double loop configurations account for 55% and 45% respectively. A short cystic artery arising from this hump may be avulsed or the RHA may be accidentally divided during dissection in the Calot’s triangle.</p><p>Demonstrating the critical view of safety will minimize bilio-vascular injuries at laparoscopic cholecystectomy. Awareness and anticipation of vascular anomalies in the hepatocystic triangle and careful dissection will minimize inadvertent injuries.</p><p><b>PP-02-279</b></p><p><b>The safety of hepatobiliary-pancreas surgery in elderly patients : retrospective analysis using comprehensive frailty assessment</b></p><p><b>Mirang Lee</b></p><p><i>Asan Medical Center/Division of Hepatobiliary and Pancreatic Surgery, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In the field of surgery, frailty is an independent risk factor for mortality, morbidity, length of stay, and postoperative complication. In an aging society, as the age of patients undergoing hepatobiliary-pancreas surgery increases and the incidence increases, we aim to investigate the impact of frailty on the occurrence of postoperative complications.</p><p><b><i>Materials and Methods:</i></b> From June 2021 to April 2024, we retrospectively analyzed 227 patients who underwent comprehensive frailty assessment among patients who underwent hepatobiliary-pancreas surgery at Asan Medical Center. We measured a 34-item frailty index constructed using Rockwood’s deficit accumulation approach. Demographics, clinicopathologic, and surgical data were retrieved for analysis.</p><p><b><i>Results:</i></b> Out of 227 patients, there were 25 frail patients and 202 non-frail patients. In the frail group, 36.0% and in the non-frail group, 5.9% received preoperative interventions including nutritional support, drug adjustment, and exercise education through preoperative geriatric consultation. There were no differences between frail and non-frail patients in complications (32.0% vs. 36.1%, p=0.684), ICU admission (20.0% vs. 15.3%, p=0.548), postoperative stay (12.6 vs. 13.7 days, p=0.576), readmission (9.1% vs. 7.6%, p=0.806).</p><p><b><i>Conclusions:</i></b> While frailty is an important factor that increases the burden after surgery, if appropriate preoperative evaluation and subsequent perioperative intervention are conducted, it should not be the absolute contraindication for hepatobiliary-pancreas surgery.</p><p><b>PP-02-280</b></p><p><b>Impact of Roux-en-Y Gastric Bypass on Esophageal Motility in Morbidly Obese Patients</b></p><p><b>Veeriya Tantatsanawong</b><sup>1</sup>, Pakkapon Rattanachaisit<sup>2,3</sup>, Pattharasai Kachornvitaya<sup>4</sup>, Suthep Udomsawaengsup<sup>4,5</sup>, Tanisa Patcharatrakul<sup>3,6</sup> and Sutep Gonlachanvit<sup>3,6</sup></p><p><sup>1</sup><i>Faculty Of Medicine Chulalongkorn University, Bangkok, Thailand;</i> <sup>2</sup><i>Department of Physiology, Faculty of Medicine, Chulalongkorn University, Thailand;</i> <sup>3</sup><i>Center of Excellence in Neurogastroenterology and Motility, Faculty of Medicine, Chulalongkorn University, Thailand;</i> <sup>4</sup><i>Department of Surgery, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Thailand;</i> <sup>5</sup><i>Treatment of Obesity and Metabolic Disease Research Unit, Faculty of Medicine, Chulalongkorn University, Thailand;</i> <sup>6</sup><i>Division of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Thailand</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Obesity increases the risk of gastroesophageal reflux disease (GERD). Roux-en-Y gastric bypass (RYGBP) effectively treats GERD in morbid obesity. This study examined esophageal motility changes before and after RYGBP.</p><p><b><i>Materials and Methods:</i></b> Morbidly obese patients (BMI ≥40 kg/m² or ≥35 kg/m² with comorbidities) planned for RYGBP were prospectively enrolled. Gastrointestinal symptoms and high-resolution esophageal manometry (HRM) were evaluated before and after surgery. Ten swallows of a standardized 5 mL liquid were performed in supine and upright positions. The Manoview software version 3.0 (Medtronic Inc., MN, USA) analyzed parameters: lower esophageal sphincter (LES), integrated relaxation pressure (IRP), distal latency (DL), and distal contractile integral (DCI).</p><p><b><i>Results:</i></b> Nine patients (age 42±11 years, BMI 44.53±4.7 kg/m²) were enrolled. Preoperatively, 40% experienced nausea and heartburn. Post-surgery, heartburn resolved. HRM was conducted with a median follow-up of 42±25 days. Patients had a significant BMI decrease (36.8±5.7 kg/m², p=0.028). Pre-surgery manometry showed normal findings with median supine IRP slightly elevated (13.35 mmHg) but normal in the upright position. Post-surgery, all manometric parameters tended to decrease without statistical significance. Preoperative LES pressure was 32.50±6.3 mmHg (upright) and 43.50±21.8 mmHg (supine); IRP was 8.73±2.6 mmHg (upright) and 13.35±6.3 mmHg (supine). Postoperative LES pressure was 24.95±6.3 mmHg (upright) and 33.35±16.1 mmHg (supine); IRP was 2.85±3.7 mmHg (upright) and 7.85±10.8 mmHg (supine). No significant change in normal peristalsis (p<0.05).</p><p><b><i>Conclusion:</i></b> There were no changes in esophageal motility post-RYGBP in morbidly obese patients. GERD symptom improvement may result from other mechanisms such as decreased intragastric pressure or accelerated gastric emptying.</p><p><b>PP-02-281</b></p><p><b>Gut Microbiota as Potential Biomarker for Metabolic dysfunction-associated fatty liver disease (MAFLD): A Systematic Review</b></p><p><b>Hery Djagat Purnomo</b><sup>1</sup>, Cecilia Oktaria Permatadewi<sup>1</sup>, Kevin Tandarto<sup>2</sup>, Hesti Triwahyu Hutami<sup>1</sup>, Didik Indiarso<sup>1</sup>, Agung Prasetyo<sup>1</sup> and Hirlan Hirlan<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Metabolic dysfunction-associated fatty liver disease (MAFLD) is the new global epidemic. Previous studies revealed the relationship between dysbiosis and MAFLD's progression via immune dysregulation and metabolic shifts. This review seeks to establish the role of gut microbiota as a biomarker for fibrosis in MAFLD.</p><p><b><i>Method:</i></b> A comprehensive search will be performed across multiple electronic databases including PubMed, Scopus, and Web of Science. The search strategy will combine relevant Medical Subject Headings (MeSH) terms and keywords related to \"gut microbiota\", \"MASLD\", “NAFLD”, “MAFLD”, \"fatty liver disease\", “Steatohepatitis”, and \"biomarker\".</p><p><b><i>Results:</i></b> A total of 16 studies were included in this review. Recent studies have extensively explored the gut microbiota's composition across various stages of MAFLD. Studies highlighted that Proteobacteria, Actinobacteria, and Verrucomicrobia were positively correlated with liver steatosis, whereas Firmicutes and Euryarchaeota showed negative correlations. Another investigation using 16S rRNA sequencing noted a decrease in Clostridium sensu stricto abundance with the onset of steatosis and fibrosis, with Escherichia/Shigella potentially serving as a discriminating microorganism for fibrosis. In Asia, a study in China using shotgun metagenomic sequencing found reduced Methanobrevibacter and Phascolarctobacterium levels, and increased Slackia and Dorea formicigenerans in MAFLD. Recent study from South Korea revealed that ET-P dominated by the genus Prevotella, constitutes a high-risk group for MAFLD in the Asian population. Japanese research noted the decreased Ruminococcaceae and Faecalibacterium, suggesting these changes as biomarkers for disease severity in Asian populations.</p><p><b><i>Conclusion:</i></b> Increasing evidence from clinical research underscores the significant involvement of gut microbiota and their metabolites in MAFLD progression</p><p><b>PP-02-284</b></p><p><b>Therapeutic Potential of Probiotics in Radiation-Induced Gastrointestinal Toxicity in Cervical and Rectal Cancer: A Meta-Analysis</b></p><p><b>Ninda Septia Yuspar</b>, Eddy Yuristo and Suyata Suyata</p><p><i>Sriwijaya University, Palembang, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Radiation-induced gastrointestinal (GI) toxicity is a significant adverse effect in patients undergoing radiotherapy for cervical and rectal cancer, often leading to severe diarrhoea and compromising treatment outcomes. Probiotics have been proposed as a therapeutic option to mitigate these toxicities.</p><p><b><i>Objectives:</i></b> To systematically review and quantitatively synthesize the evidence on the therapeutic potential of probiotics in reducing radiation-induced GI toxicity, specifically focusing on the incidence of diarrhoea and severe diarrhea in patients with cervical and rectal cancer.</p><p><b><i>Methods:</i></b> A comprehensive literature search was conducted in PubMed, Embase, and Cochrane Library databases to identify studies assessing the impact of probiotics on radiation-induced GI toxicity. Inclusion criteria were studies reporting on the incidence of diarrhoea and severe diarrhoea, defined by the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) as grade ≥3. Data were extracted and pooled using a random-effects model, and odds ratios (OR) were calculated. Heterogeneity was assessed using the I² statistic.</p><p><b><i>Results:</i></b> A total of 7 studies, including 1103 patients, met the inclusion criteria. The pooled analysis showed that probiotics insignificantly reduced the incidence of diarrhoea (OR 0.71, 95% confidence interval [CI] 0.38-1.33) and had a significant effect on reducing severe diarrhoea (OR 0.22, 95% CI 0.07-0.69). Heterogeneity among studies was moderate (I² = Z%).</p><p><b><i>Conclusion:</i></b> This meta-analysis indicates that probiotics have potential therapeutic benefits in reducing radiation-induced GI toxicity in patients with cervical and rectal cancer, particularly in decreasing the incidence of severe diarrhea.</p><p><b><i>Keywords:</i></b> probiotics, radiation-induced gastrointestinal toxicity, cervical cancer, rectal cancer, severe diarrhea</p><p><b>PP-02-306</b></p><p><b>Compassion fatigue, burnout and professional quality of life among organ transplant coordinators: A descriptive study</b></p><p><b>Aye Su Mon</b></p><p><i>National University Centre for Organ Transplantation, Singapore</i></p><p><b><i>Objectives:</i></b> To access compassion fatigue, burnout and professional quality of life among liver and kidney transplant coordinators.</p><p><b><i>Materials and Methods:</i></b> A non-experimental, exploratory descriptive study was carried out by using Professional Quality of Life Scale Version 5 (ProQOL-V) among liver and kidney transplant coordinators in one of the transplant centers in Singapore. A total of 11 transplant coordinators participated in this study.</p><p><b><i>Results:</i></b> Based on the responses, there is moderate level of compassion fatigue, burnout is present among the transplant coordinators. But, on the other hand, there is an average level of compassion satisfaction for being able to help patients as transplant coordinators.</p><p><b><i>Conclusion:</i></b> Healthy and supportive work environment play an essential role in the mental well-being of healthcare professionals which in turn contributes to overall patients’ care quality, enhanced job satisfaction and organizational success. Studies with a greater number of participants will be required for better understanding and for implementation of necessary changes.</p><p><b>PP-02-314</b></p><p><b>Numb mitigates radiation-induced intestinal epithelial cell senescence via a Polo-like kinase 1-dependent pathway</b></p><p><b>Yongtao Yang</b><sup>1</sup>, Xiao Hu<sup>1</sup>, Yuwei Pan<sup>1</sup> and Tao Liu<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Chongqing University Cancer Hospital, Chongqing, China;</i> <sup>2</sup><i>Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to explore the impact of Numb on senescence of intestinal epithelial cells and injury to the intestinal mucosal barrier by modulating Plk1 activity following radiation.</p><p><b><i>Materials and Methods:</i></b> C57BL/6J mice and colonic cells were exposed to radiation to simulate radiation-induced intestinal injury in vivo and in vitro. Intraperitoneal injection of Numb antisense oligodeoxynucleotides was used to downregulate Numb expression in mouse intestinal epithelial cells. Intestinal tissue pathology was evaluated through histological staining. Intestinal mucosal barrier permeability was evaluated through FITC-dextran assay and serum DAO concentration. Gene and protein expression levels were assessed using quantitative RT-PCR, immunoblotting and immunohistochemistry, while flow cytometry was employed to analyze apoptosis rates and cell cycle progression.</p><p><b><i>Results:</i></b> Inhibiting Numb expression in intestinal epithelial cells exacerbated histopathological damage to the intestinal mucosa post-radiation, leading to increased intestinal mucosal barrier permeability. This inhibition also resulted in elevated levels of γH2AX, p21, and SA-β-Gal in intestinal epithelial cells, along with enhanced expression of inflammatory factors. In vitro experiments revealed that interfering with Numb expression led to an accumulation of colon cells in the G2 phase, promoting cell senescence and upregulating inflammatory factors. Additionally, interfering with PLK1 expression was found to enhance radiation-induced intestinal cell senescence, while overexpressing PLK1 could reverse this senescence induced by Numb knockdown.</p><p><b><i>Conclusion:</i></b> This study highlights the crucial role of Numb in safeguarding intestinal mucosal barrier function by inhibiting radiation-induced senescence of intestinal epithelial cells. Modulating the Numb-PLK1 signaling pathway may present a promising therapeutic target for radiation enteritis.</p><p><b>PP-02-315</b></p><p><b>Clinicopathological characteristics, immunohistochemical findings, and genetic mutations in carcinoma of the pancreatic neck</b></p><p><b>Yoshihiro Hamada</b> and Yusuke Ishida and Masatoshi Kajiwara</p><p><i>Fukuoka University Hospital, Fukuoka, Japan</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The pancreatic neck (Pn) is an important region because it is located next to important blood vessels like the portal vein, superior mesenteric vein, common hepatic artery, and gastroduodenal artery, and it forms the resection line in pancreatoduodenectomy or distal pancreatectomy for pancreatic neoplasms. We analyzed the clinicopathological characteristics, immunohistochemical findings, and genetic mutations of IDCPn.</p><p><b><i>Methods:</i></b> Among 487 patients diagnosed with IDCP, 4 (5.2%) had tumors located in the Pn with diameter <2 cm.</p><p><b><i>Results:</i></b> The IDCPn patients comprised two men and two women with an age range of 67–75 years. Two patients underwent pancreatoduodenectomy, and two patients underwent total pancreatectomy. All four tumors were moderately differentiated adenocarcinoma. The four tumors had obvious carcinoma in situ (CIS) infiltration (24–56 mm). One patient had regional lymph node metastasis. The tumor cells showed p53 abnormalities were recognized in all patients, but neither p16 inactivation nor DPC4 inactivation was observed. KRAS mutations on codons 12 and 61 were not detected in the patients. Despite a lack of intraductal papillary mucinous neoplasm (IPMN) findings on preoperative imaging or pathological examinations, GNAS mutations at codon 201 were detected in two patients. One patient died of liver metastasis, two patients remained alive without recurrence, and one patient died of another cause.</p><p><b><i>Conclusion:</i></b> Surgical resection of IDCPn should be conducted carefully because IDCPn can have extended CIS. IDCPn showed typical morphological findings of IDCP, but its carcinogenesis and progression may differ from IDCP at other anatomical sites based on the genetic mutation analysis.</p><p><b>PP-02-316</b></p><p><b>Morphological severity in the chronic pancreatitis could guess the severity of the pancreatic exocrine insufficiency</b></p><p><b>Min Kyu Jung</b><sup>1</sup> and Byung Ik Jang<sup>2</sup></p><p><sup>1</sup><i>Kyungpook National University School Medicine, Daegu, South Korea;</i> <sup>2</sup><i>Yeungnam University College of Medicine, Daegu, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> The association between pancreatic exocrine insufficiency (PEI) and morphologic findings in chronic pancreatitis has not been fully studied yet. Thus, the aim of this study was to investigate the correlation between PEI severity and computed tomography (CT)-based morphological severity in patients with chronic pancreatitis.</p><p><b><i>Methods:</i></b> This multicenter retrospective study included 180 patients with chronic pancreatitis aged 18 years or older between January 2018 and December 2021. PEI severity was measured by PEI questionnaire (PEI-Q). Morphological severity was measured using a CT-based scoring system including pancreatic duct caliber, pancreatic duct stricture or intraductal obstructing calculus, pancreatic atrophy, and pancreatic calcification. In addition, 35 patients who received pancreatic enzyme replacement therapy (PERT) were evaluated by PEI-Q to determine whether PEI improved after PERT.</p><p><b><i>Results:</i></b> PEI severity was normal (n = 89), mild (n = 69), moderate (n = 14), or severe (n = 8). Severities of pancreatic duct caliber and pancreatic duct stricture or intraductal obstructing calculus had small but significant associations with PEI severity (Cramer’s V = 0.121 and 0.141, respectively). Severities of pancreatic atrophy and pancreatic calcification were not significantly associated with PEI severity. PEI severity showed a significant improvement after PERT (P < 0.001).</p><p><b><i>Conclusions:</i></b> PEI severity had significant associations with CT-based morphological severities, including severities of pancreatic duct caliber and pancreatic duct stricture or intraductal obstructing calculus. In addition, PEI-Q could be a useful indicator for evaluating therapeutic effect of PERT in clinical practice.</p><p><b>PP-02-317</b></p><p><b>Natural History of Infected Pancreatic Necrosis(IPN) - Prospective evaluation of management challenges in clinical practice</b></p><p><b>Jahangeer Basha Medarapalem</b>, Gauri Nayak, Zahher Nabi, Mohan Ramchandani, Rajesh Gupta, Rupjyoti Talukdar, Nitin Jagtap, Sundeep Lakhtakia and D Nageshwar Reddy</p><p><i>Asian Institute Of Gastroenterology, Hyderabad, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> One critical factor in deciding the outcome of Infected Pancreatic Necrosis(IPN) is the incorporation of an appropriate treatment strategy at the right time in the course of Acute Necrotizing Pancreatitis(ANP). We aimed to evaluate the management strategies followed using a standard treatment protocol in a cohort of IPN patients.</p><p><b><i>Methods:</i></b> This was a prospective observational study that included consecutive patients of ANP with IPN from January-December 2022. Patients with IPN who didn’t improve with antibiotics were considered for drainage. Assessment for EUS-drainage with LAMS(Lumen Apposing Metal Stents) was attempted as a first preference. If EUS-drainage not feasible, percutaneous catheter drainage(PCD) was considered, as a part of step-up approach. The outcomes measured - treatment strategy adopted, timing of intervention, clinical outcome, and mortality.</p><p><b><i>Results:</i></b> 117 patients were included in the study (males 62%, mean age-37.46 years). 38 patients(32%) could be managed conservatively, remaining 79(68%) patients required intervention. Initial drainage performed was EUS-guided in 30 (38%) while 49 (62%) underwent PCD. Early drainages(<4 weeks) were performed in 30/79 patients (38%), of which EUS drainage was possible in 8, while the majority (22) underwent PCD (10% vs 28%; p=0.06). The overall mortality was 20.5%. There was no significant difference in mortality between EUS drainage (22.2%) and PCD patients (30%).</p><p><b><i>Conclusion:</i></b> PCD remains the primary modality both in early(< 4 weeks) and overall drainages in IPN despite efforts to consider EUS drainage. Adopting the specific modality of drainage is decided by the feasibility rather than choice in real world clinical practice.</p><p><b>PP-02-318</b></p><p><b>Analysis of bile acid composition in recurrent bile duct gallstone patients</b></p><p>Kihyun Ryu and <b>Taehee Lee</b></p><p><i>Konyang University Hospital, Daejeon, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract Body</b></p><p><b><i>Background/Aims:</i></b> Among bile duct gallstone patients encountered in clinical practice, patients who recur after initially removing bile duct gallstone account for approximately 20%. The purpose of this study is to determine whether differences in the composition of bile acids affects the recurrence of bile duct gallstone.</p><p><b><i>Methods:</i></b> From 2019 to 2022, at a single institution at Konyang University Hospital, the composition of bile from patients who underwent bile drainage for biliary tract diseases was investigated for 15 types of bile acids. Five of them are unconjugated bile acids; cholic acid (CA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA) and lithocholic acid (LCA). The remaining 10 are conjugated bile acids formed by combining with taurine and glycine.</p><p><b><i>Results:</i></b> Of the total 184 patients with bile duct gallstone, 155 were in the first-diagnosis group and 29 were in the recurrent group. The mean age (years) was significantly older in the recurrent group than the first-diagnosis group (77.79 vs. 68.96, P=0.005). Among the conjugated bile acids, the quantitative values (ppm) of tauro-CA, tauro-DCA, glyco-CA, glyco-DCA, and glyco-LCA were significantly higher in the first-diagnosis group.</p><p><b><i>Conclusion:</i></b> The recurrence of bile duct gallstone was significantly higher in older patients, and significant differences were shown in the composition of several bile acids. The low amount of bile acid production through the classical pathway in the recurrent group means that the classical pathway mainly occurs in the liver, so the amount of bile acid produced in the liver is especially low.</p><p><b>PP-02-328</b></p><p><b>Effectiveness of Saroglitazar in MASLD associated steatosis and fibrosis-An open label, real world study</b></p><p><b>Manoj Kumar Agarwal</b><sup>1</sup>, Sandeep Gupta<sup>1</sup>, Abhinav Agarwal<sup>2</sup> and Chandni Agarwal<sup>1</sup></p><p><sup>1</sup><i>Belle Vue Clinic, Kolkata, India;</i> <sup>2</sup><i>SMSMC, Jaipur, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Saroglitazar, a dual PPARα/γ agonist, has shown promising potential in the management of MASLD and MASH (metabolic dysfunction-associated steatohepatitis). We primarily aimed to evaluate the efficacy of Saroglitazar 4 mg OD on fibrosis and steatosis by assessing changes in liver stiffness measurement(LSM) and controlled attenuation parameter(CAP) using transient elastography(TE).</p><p><b><i>Materials and Methods:</i></b> A cohort of MASLD patients (n=114, 68.4% male, 60.5% with F3-F4 fibrosis[LSM>10kPa], and mean age 51.9±12.4 years) on Saroglitazar were assessed for changes in fibrosis(LSM), steatosis(CAP), and liver enzymes at 24 and 52 weeks. The baseline mean LSM was 13±6.4 kPa, with 69(60.5%) having advanced(F3-F4) fibrosis. The paired sample t-test was used to assess changes from baseline.</p><p><b><i>Results:</i></b> The study showed statistically significant improvements in LSM(kPa) by 2.23(17.2%, 13 ±6.4 to 10.8 ±4.6, p <0.05) and 3.7(28.1%, 13 ±6.4 to 9.4 ±3.7, p<0.001), in CAP(dB/m) by 30.3 (9.5%, 317.8 ±45.9 to 287.5 ±44.4, p<0.001) and 36.4(11.5%, 317.8 ±45.9 to 281.3 ±40.8, p<0.001), in ALT by 38.8 (46.6%, 83.3 ±47.3 to 44.5 ±24.8, p<0.001) and 51.7 (62.1%, 83.3 ±47.3 to 31.6 ±14.02, p<0.001), and in AST by 36.9(45.6%, 80.8 ±42.2 to 43.9 ±20.9, p<0.001) and 46.6 (57.7%, 80.8 ±42.2 to 34.2 ±12.8, p<0.001), at 24 and 52 weeks respectively.</p><p><b><i>Conclusion:</i></b> Saroglitazar was found to be effective in reducing LSM and CAP, as well as liver enzyme levels SGOT and SGPT. It was also found to be equally effective in advanced stages of MASH-related fibrosis. Future well-designed randomized controlled studies will provide more strength to these findings.</p><p><b>PP-02-331</b></p><p><b>Increasing awareness among primary care physicians about MASLD reduced unnecessary referral rates</b></p><p>Eileen L Yoon<sup>1</sup>, Sang Bong Ahn<sup>2</sup> and <b>Dae Won Jun</b><sup>1</sup></p><p><sup>1</sup><i>Hanyang University, Seoul, South Korea;</i> <sup>2</sup><i>Eulji University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> This study aimed to assess the impact of an online educational program on MASLD for physicians and its influence on modifying their clinical practice patterns.</p><p><b><i>Methods:</i></b> A total of 869 physicians [72 physicians at referral centers and 797 primary care physicians (PCPs)], who consented to receive educational materials on MASLD, participated in this study. They completed an initial survey regarding their clinical practices for patients with MASLD, followed by a second online survey eight weeks after receiving a series of seven weekly educational materials on MASLD.</p><p><b><i>Results:</i></b> Frequency of utilizing noninvasive tests for liver fibrosis, used as the first tier evaluation in both physicians at referral centers and PCPs, was low at 6.9% and 6.9%, respectively, and there was no difference between the two groups. Regarding barriers to the management of MASLD, specialists in referral centers considered the ‘short consultation time’ as the primary challenge, while PCPs cited the ‘the absence of a fee for this service’ as the major barrier. Additionally, the proportion of PCPs considering noninvasive tests testing for patients in the 'at-risk group' increased significantly for those with diabetes (32.6% to 38.5%), cardiovascular disease (9.2% to 16.1%), and ischemic stroke (8.4% to 11.5%). After education, the percentage of PCPs who immediately referred patients to a specialist after a MASLD diagnosis decreased from 15.4% to 12.3% (P value = 0.042).</p><p><b><i>Conclusions:</i></b> Increasing awareness among PCPs about MASLD reduced unnecessary referral rates and an increased cardiovascular evaluation.</p><p><b>PP-02-332</b></p><p><b>Diagnostic performances of FIB-4 and NFS in MASLD/MAFLD in primary care clinic of Asia</b></p><p>Eileen L Yoon<sup>1</sup>, Sang Bong Ahn<sup>2</sup> and <b>Dae Won Jun</b><sup>1</sup></p><p><sup>1</sup><i>Hanyang University, Seoul, South Korea;</i> <sup>2</sup><i>Eulji University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and aims:</i></b> We aimed to explore extent to which individuals previously diagnosed with nonalcoholic fatty liver disease (NAFLD) meet the criteria fulfilled with the new nomenclature, metabolic dysfunction-associated steatotic liver disease (MASLD), within an Asian primary care cohort. Additionally, we assessed the reliability of the diagnostic performance of FIB-4 and NFS for MASLD within the primary care clinic cohort.</p><p><b><i>Methods:</i></b> This retrospective cross-sectional study included participants who underwent 6,740 magnetic resonance elastography (MRE) and abdominal ultrasonography during their health checkups at nationwide health promotion centers.</p><p><b><i>Results:</i></b> The prevalence rates of NAFLD and MASLD diagnosed based on ultrasonography results were 36.7% and 38.0%, respectively. Notably, 96.8% of patients in the NAFLD cohort fulfilled the new criteria for MASLD. A small proportion of patients with NAFLD (n = 80, 3.2%) did not meet the MASLD criteria. Additionally, 168 patients (6.6%) were newly added to the MASLD group. The areas under the receiver operating characteristic curves for diagnosing advanced hepatic fibrosis for FIB-4 (0.824 in NAFLD vs. 0.818 in MASLD, P = 0.891) and NFS (0.803 in NAFLD vs. 0.781 in MASLD, P = 0.618) were comparable between the MASLD and NAFLD. Furthermore, the sensitivity, specificity, positive and negative predictive value of FIB-4 and NFS for advanced fibrosis in MASLD were also comparable to those in NAFLD.</p><p><b><i>Conclusions:</i></b> Almost patients (96.8%) previously diagnosed with NAFLD fulfilled the new criteria for MASLD in an Asian primary clinic cohort. Diagnostic performance of FIB-4 in the primary care MASLD cohort demonstrated satisfactory results.</p><p><b>PP-02-337</b></p><p><b>Anti-inflammatory effect of MLKL ATP binding inhibitor via necroptosis independent pathway in alcoholic liver disease.</b></p><p>Eileen L Yoon<sup>1</sup>, Sang Bong Ahn<sup>2</sup> and <b>Dae Won Jun</b><sup>1</sup></p><p><sup>1</sup><i>Hanyang University, Seoul, South Korea;</i> <sup>2</sup><i>Eulji University, Seoul, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Mixed Lineage Kinase Domain Like Pseudokinase (MLKL) induces cell death through the stimulation of necroptosis. Recent studies have demonstrated that the necroptosis-independent pathway exhibits anti-inflammatory effects. Our objective is to investigate whether an MLKL inhibitor can attenuate liver disease through the necroptosis-independent anti-inflammatory pathway.</p><p><b><i>Method:</i></b> Cell viability was observed following the induction of necroptosis in RAW cells. Additionally, the expression of chemokines and adhesion molecules was analyzed after inducing necroptosis in RAW cells. Compound-4, identified as an MLKL inhibitor binding to the ATP binding site, was administered to animal models with non-alcoholic fatty liver disease and alcoholic liver disease, despite lacking the ability to inhibit necroptosis.</p><p><b><i>Results:</i></b> In contrast to HT29 cells, RAW cells demonstrate the ability to evade cell death in response to necroptosis stimuli. RAW cells exhibit no MLKL phosphorylation and activation after necrotic stimulation; however, they can induce necrosis-associated cell death following demethylation. While an MLKL ATP pocket binding inhibitor does not prevent cell death induced by necroptosis in RAW cells, it does reduce the expression of CXCL2, ICAM, and VCAM resulting from necroptosis. Notably, this inhibitor diminishes the expression of CXCL2, ICAM, and VCAM by inhibiting the IKKb and NFkB pathways without inducing cell death through necroptosis. Furthermore, the MLKL ATP binding inhibitor, Compound-4, demonstrated attenuation of hepatic inflammation in alcoholic animal models.</p><p><b><i>Conclusion:</i></b> MLKL ATP pocket binding inhibitor has an anti-inflammatory effect through the necroptosis independent pathway in liver disease.</p><p><b>PP-02-339</b></p><p><b>Sorafenib Resistance in Hepatocellular Carcinoma: A Bibliometric Analysis from 2008 to 2024</b></p><p><b>Mao-sheng Liu</b><sup>1</sup>, Si-Si Zhong<sup>2</sup>, Jin-Ke Wang<sup>1</sup>, Ting Wang<sup>1</sup> and Kun-He Zhang<sup>1</sup></p><p><sup>1</sup><i>The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China;</i> <sup>2</sup><i>The First Affiliated Hospital of Gannan Medical University, Ganzhou, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Resistance to sorafenib in hepatocellular carcinoma (HCC) treatment is common, and a systematic and comprehensive presentation of the research trends and hotspots in this field can assist researchers in developing more comprehensive treatment strategies.</p><p><b><i>Materials and methods:</i></b> Using the keywords \"hepatocellular carcinoma\", \"sorafenib\" and \"resistance\", we searched the Web of Science database for relevant literature published between 2008 and 2024. Eligible articles in this field were included, and quantitative analysis and visualization were performed using bibliometric analysis software VOSviewer and Citespace. The contributions of countries, institutions, authors, and journals in this field were analyzed, as well as highly cited articles, research trends, and hotspots. Figure 1 shows the detailed information in the literature collection and analysis.</p><p><b><i>Results:</i></b> A total of 1,534 eligible literatures were included in the study. The annual publication and citation counts gradually increased and reached a peak in 2022. China is the country with the largest contribution in this field, with Fudan University as the institution with the highest number of publications and Chen Kuen-Feng as the most influential researcher. Current research hotspots include cancer stem cells and stemness, hypoxia and glycolysis, autophagy and ferroptosis, tumor microenvironment, and immune checkpoint inhibitors. The research frontiers are tumor microenvironment and immunotherapy.</p><p><b>PP-02-340</b></p><p><b>Diagnostic Value of Multivalent Serum Aptamers for Early Hepatocellular Carcinoma</b></p><p><b>Mao-sheng Liu</b>, Jin-Ke Wang, Ding-Fan Guo, Qi Wen, Yun-Hui Liang, Ting Wang and Kun-He Zhang</p><p><i>The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The current blood-based diagnostic methods for early-stage hepatocellular carcinoma (eHCC) are inadequate for clinical application due to their suboptional performance. Previously, we generated a group of aptamers against HCC sera. In this study, we aimed to evaluate the diagnostic value of nanoparticle-based multivalent HCC serum aptamers for eHCC.</p><p><b><i>Materials and methods:</i></b> Serum samples and clinical data were collected from eHCC patients (n=104), chronic liver disease patients (cirrhosis and hepatitis) (n=184), and healthy controls (n=96) at our hospital. Using our previously developed aptamer-based triple serum fluorescence intensity (ATSFI) assay, we sequentially measured the autofluorescence of 5μL serum, the fluorescence after adding 5μL EvaGreen dye (16×), and the fluorescence after adding 5μL multivalent HCC serum aptamers (0.2pmol/μL). The diagnostic performance of the original and derived fluorescence indicators for eHCC was assessed using the area under the receiver operating characteristic curve (AUROC).</p><p><b><i>Results:</i></b> The highest AUROCs among single fluorescence indicators were 0.863 for distinguishing eHCC from chronic liver disease, 0.919 for distinguishing eHCC from healthy controls, and 0.887 for distinguishing eHCC from non-HCC individuals (chronic liver disease and healthy controls). The AUROC of AFP was 0.732 for eHCC vs. non-HCC. Subgroup analysis showed AUROCs of 0.903, 0.889, and 0.907 for distinguishing AFP-negative eHCC, small eHCC, and poorly differentiated eHCC from non-HCC individuals, respectively. Logistic stepwise regression modeling using the ATSFI indicators yielded corresponding AUROCs of 0.936, 0.948, and 0.950, respectively.</p><p><b><i>Conclusion:</i></b> Multivalent HCC serum aptamers are valuable for the diagnosis of eHCC, including AFP-negative, small, and poorly differentiated eHCCs.</p><p><b>PP-02-341</b></p><p><b>A Comparison of Safety and Efficacy of Elobixibat and Lubiprostone in Chronic Constipation:A Systematic Review</b></p><p><b>Yulisa Tanjung</b></p><p><i>Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, M, Medan, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>A Comparison of Safety and Efficacy of Elobixibat and Lubiprostone in Chronic Constipation: A Systematic Review</p><p>YULISA Astari Tanjung [1]; MASRUL Lubis [2]; IMELDA rey [3]; TAUFIK Sungkar [4]; ILHAMD Ilhamd [5]; DARMADI Darmadi [6]</p><p>1-Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; 2-Division of Gastroenterohepatology, Department of Internal Medicine</p><p><b>Abstract</b></p><p><b><i>Objectives:</i></b> Constipation is one of major gastrointestinal disorders worldwide. Chronic constipation impairs patients' quality of life. Previous treatment options for chronic constipation show unsatisfactory outcome. This systematic review aims to compare efficacy and safety between elobixibat and lubiprostone in patients with chronic constipation.</p><p><b><i>Materials and methods:</i></b> Literature searching was conducted in multiple databases. Inclusion criteria were studies involving patients with chronic constipation, utilization of elobixibat or lubiprostone, and reporting efficacy and safety outcomes. Data was analyzed using Microsoft Excel software.</p><p><b><i>Results:</i></b> A total of 6 studies with 2,949 patients was included in this study. A total of 3 studies reported symptom’s improvement after administration of lubiprostone compared to placebo while 2 studies showed similar findings after administration of elobixibat. Adverse events were less common in elobixibat compared to lubiprostone.</p><p><b><i>Conclusion:</i></b> Elobixibat and lubiprostone are effective in treating chronic constipation. Elobixibat shows superiority in safety profile compared to lubiprostone.</p><p><b><i>Keywords:</i></b> constipation, efficacy, elobixibat, lubiprostone, safety</p><p><b>PP-02-342</b></p><p><b>Chronic Abdominal Pain Due to Liver Infarction Initially Suspected as Hepatoma : A Rare Case</b></p><p><b>Nyimas Maida Shofa</b><sup>1</sup>, Tri Asih Imro'ati<sup>2</sup>, Budi Widodo<sup>1</sup> and Titong Sugihartono<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroentero-Hepatology, Department of Internal Medicine, Airlangga University – Dr. Soetomo General Hospital, Surabaya, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine University Airlangga Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Liver infarction are extremely rare because the liver receives blood from various sources. Liver infarction has an unknown cause. The lack of distinct imaging appearances and clinical signs typically results in a misdiagnosis and poor prognosis. It is unclear how best to treat these infarctions once they are diagnosed</p><p><b><i>Case Description:</i></b> A 66-year-old man complained of persistent pain in the right upper abdomen, nausea and appetite loss. Abdominal ultrasonography demonstrated irregular edge hypoechoic nodules size 2.2cm in diameter with suspected hepatoma. Liver function and virus marker tests within normal limits. Computed tomography examination resulted in a 2.4x3.6x1.6 cm hypodensities lesion in the 6th segment of the right lobe of the liver which, when given contrast, was neither wash in nor wash out contrast on the intralesion. Seen arterial enhancement on in the extralesion, consistent with hepatic infarction. Anticoagulant therapy was chosen as the treatment strategy. Patients were given rivaroxaban. Swift resolution of the abdominal pain was noted within a month. The patient continued treatment for six months</p><p><b><i>Discussion:</i></b> The causes of liver infarction are complex, and can even be fatal. Simple imaging, such as ultrasound, CT scan, or magnetic resonance imaging, is sometimes insufficient. In addition, detailed history taking, physical examination, and related serum studies are also crucial. We should also be aware of the clinical characteristics, imaging appearance, and serum results of other liver lesions, such as liver cancer. Anticoagulant therapy can be considered in the treatment of liver infarction of unknown etiology.</p><p><b>PP-02-343</b></p><p><b>Efficacy of Saroglitazar on Liver fibrosis in MASLD:An open label real world study</b></p><p><b>Sujit Chaudhuri</b> and Agnibha Dutta</p><p><i>Manipal Hospital, Broadway, Kolkata, India</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Liver stiffness measurement (LSM) by transient elastography(TE) is a valuable tool in the non-invasive assessment and management of liver fibrosis in chronic liver diseases(CLD). Saroglitazar, a dual PPARα/γ agonist, is approved in India for management of MASLD(metabolic dysfunction associated Steatotic liver disease)with evidence of fibrosis. We evaluated the effect of Saroglitazar in different fibrosis stages of MASLD.</p><p><b><i>Materials and Methods:</i></b> 133 documented MASLD patients on Saroglitazar treatment(male 70.3%(92), non-diabetic 52.3%(68), non-hypertensive 46.1%(59), non-dyslipidemic 71.2%(89), 38.3%(51) with LSM>14 kPa) were evaluated for changes in mean liver stiffness(LSM) and steatosis (CAP) from baseline to follow-up visits till 96 weeks. Paired sample t-test at 95% CI was applied and p<0.05 is considered as statistical significant. Safety was assessed at every follow up visit based on the frequency and severity of any adverse events.</p><p><b><i>Results:</i></b> The mean improvement in LSM was 6.9kPa(42.6%,p<0.001) from 16.2±12.4 to 9.3±4.8 and in CAP was 59.7dB/m(19.3%,p<0.001) from 309.6±48.9 to 249.8±49.8, after 96 weeks. In cohort analysis based on fibrosis stages(F0-F4), mean LSM improvement in cohort1(G1,LSM<7.5kPa(F0-F1),n=20) was 0.61(9.3%,p=0.03) from 6.5±0.68 to 5.9±1.04; in cohort2(G2,LSM7.5-10kPa(F2),n=39) was 1.73(20.1%,p<0.001) from 8.7±0.67 to 6.9±2.6; in cohort3(G3,LSM10-14kPa(F3),n=23) was 3.35(28.1%,p<0.001) from 11.95±0.9 to 8.6±3.7; and in cohort4(G4,LSM>14kPa(F4),n=51) was 15.3(54.6%,p<0.001) from 28±13.3 to 12.7±5.3.There was no major side effect during the treatment except 2 brief incidences (skin rash and loose motion).</p><p><b><i>Conclusion:</i></b> Saroglitazar is found to be effective in fibrosis reduction across the stages from F0-F1 to F4 in MASLD. Further well controlled randomized studies are needed to support the finding of this study.</p><p><b>PP-02-344</b></p><p><b>Early and Late-Onset Colorectal Cancer in African Americans During COVID-19</b></p><p>Hassan Brim<sup>1</sup>, Lakshmi Chirumamilla<sup>1</sup>, Challa Suryanarayana Reddy<sup>1</sup>, Adeyinka Laiyemo<sup>1</sup>, Reza Oskrochi, Mrinalini Deverapall<sup>1</sup>, Rumaisa Rashid, Rabia Zafar<sup>1</sup> and Mudasir Rashid<sup>1</sup> and Babak Shokrani<sup>1</sup> and <b>Hassan Ashktorab</b><sup>1</sup></p><p><sup>1</sup><i>Howard University, Washington, United States;</i> <sup>2</sup><i>American Middle East University, Kuwait</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The incidence of early-onset colorectal cancer (EOCRC, <45 years of age at onset) is on the rise among adults, including African Americans (AA).</p><p><b><i>Aim:</i></b> To examine differences between EOCRC and late-onset colorectal cancer (LOCRC) among AA patients and any effect during COVID by comparing data during pre-covid (2015-2019) and the COVID era (2020-2023).</p><p><b><i>Methods:</i></b> We conducted a retrospective review of Howard University Hospital records from 2015 to 2023 for CRC patients that included demographics, clinicals, pathology, and colonoscopy records. A three-year interval analysis was performed to compare post-COVID era (2020-2023) to preceding years to discern temporal trends.</p><p><b><i>Results:</i></b> 138 LOCRC and 13 EOCRC cases of which >80% of patients were AA. Compared to pre-COVID, LOCRC cases increased in number from 55 to 83, and EOCRC cases increased from 6 to 7 during COVID. There was no change in mean age for LOCRC (64.7 vs. 65.3 years) but mean age increased for EOCRC (37.3 vs. 41.5 years). Males predominated in both groups particularly during the pandemic. More than 65% of LOCRC patient colonoscopies were for diagnostic purposes. GI bleeding as a colonoscopy indication and reduced bowel preparation quality were increased during the pandemic. EOCRC patients showed a shift from stage 4 (49.2%) to stage 2 (30%) and LOCRC patients staging trends changed from stage 4 (40%) to stage 3 (28.6%).</p><p><b><i>Conclusion:</i></b> We report increase in CRC cases during the COVID-19 era, especially among young AA males. EOCRC and LOCRC patients showed distal location predominance, most commonly in recto-sigmoid region.</p><p><b>PP-02-345</b></p><p><b>Comparative diagnostic performance of RUT with the sweeping versus conventional method after Helicobacter pylori eradication</b></p><p><b>Kee Lee</b> and Jae Keun Kim and Choong Kyun Noh and Kil Ho Lee and Byung Moo Yoo</p><p><i>Ajou University School Of Medicine, Suwon, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> The rapid urease test (RUT) is widely used to detect Helicobacter pylori (H. pylori) infection; however, it is not preferred as a monitoring strategy following eradication owing to its low sensitivity. In this study, we evaluated the diagnostic performance of RUT using the sweeping method, which overcomes the limitations of conventional tissue sampling methods following eradication.</p><p><b><i>Methods:</i></b> Patients who received H. pylori eradication treatment were enrolled. Each of the sweeping and conventional methods was performed on the same patients to compare diagnostic performance. Urea breath test (UBT), histology, and PCR were performed to determine true infection. Logistic regression analysis was conducted to investigate reasons for discrepancies between the results of the two methods.</p><p><b><i>Results:</i></b> In 216 patients, the eradication success rate was 68.1%, sensitivity and specificity of the sweeping method were 0.812 and 0.912, respectively, whereas those of the conventional method were 0.391 and 0.993, respectively (P<0.05 for all). AUROC for the sweeping method was higher than that for the conventional method (0.862 vs. 0.692; P<0.001). The mean time to H. pylori detection for the sweeping method was 4.7±4.4 min and 12.3±16.1 min for the conventional method (P<0.001). The risk for inconsistent results between the two methods was the highest in the UBT value 1.4–2.4‰ (OR 3.8, P=0.016).</p><p><b><i>Conclusions:</i></b> The RUT with the sweeping method could potentially replace the tissue sampling method as a test to confirm H. pylori eradication and be an alternative option to UBT for patients requiring endoscopy.</p><p><b>PP-02-346</b></p><p><b>Slc26a9 governs gastric parietal cell identity and has an important role in the injury-induced metaplasia</b></p><p><b>Bei Ji</b><sup>1</sup>, Zhiyuan Ma<sup>1</sup>, Zilin Deng<sup>1</sup>, Enqing Wu<sup>1</sup>, Jiaxing Zhu<sup>1</sup>, Biguang Tuo<sup>1</sup>, Taolang Li<sup>2</sup> and Xuemei Liu<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China;</i> <sup>2</sup><i>Department of general surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gastric injury induces metaplasia is based on parietal cells loss and reprogramming chief cells into proliferative, mucin-rich spasmolytic polypeptide-expressing metaplasia (SPEM) cells. Slc26a9 is a key regulator to maintain mucosal homeostasis. However, the exact role of Slc26a9 in injury-induced metaplasia is need further investigated.</p><p><b><i>Methods:</i></b> PCR and histopathological and immunofluorescence (IF).</p><p><b><i>Results:</i></b> We first demonstrated that Slc26a9 is expressed in all early gastric progenitors and strongly expressed in mature parietal cells. In addition mice with selective Slc26a9 deficiency in parietal cells exhibited SPEM based on autoimmune atrophic gastritis and significant up-regulation of parietal death-related markers including GSDMD, Caspase- 1, ASC, IL-1β, IL-18, and NLRP3 expression, with activated GSDMD leading to mitochondrial mediators of ACO2 leading to the death of parietal cells, followed Tuft cell migration and release of the alerting hormone IL-25, which activates ILC2 cells and promotes the activation of the IL-25-ILC2-M2 macrophage axis, resulting in the development and progression of SPEM. Also in human SPEM tissues, Slc26a9 significantly downregulated and the IL-25-macrophage M2 polarization marker CD163+ was significantly upregulated. These results suggest that deletion of the gastric parietal cell-specific Slc26a9 gene leads to parietal cell death by regulating GSDMD-mediated mitochondrial damage; and the development of SPEM through the modulation of IL-25-ILC2-M2-type macrophage polarization.</p><p><b><i>Conclusions:</i></b> We identify Slc26a9 as a potential key regulatory gene for parietal cells. Slc26a9 plays an essential protective role in stabilizing gastric mucosal immunity and gastric epithelial cell homeostasis during injury-induced chemotaxis.</p><p><b>PP-02-347</b></p><p><b>Comparison of Synergistic Sedation With Midazolam and Propofol Versus Midazolam and Pethidine in Colonoscopies</b></p><p><b>Hyun-soo Kim</b>, Dong Hyun Kim, Jae Woong Lim, Min Jae Kim and Gang Han Lee</p><p><i>Chonnam National University Hospital And Medical School, 42, Jebong-ro, Dong-gu, Gwangju, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Colonoscopy is crucial for the early detection of colorectal cancer. However, discomfort associated with this procedure often necessitates sedation, and the optimal sedation method remains unclear.</p><p><b><i>Methods:</i></b> We conducted a prospective randomized controlled study in which patients undergoing colonoscopy were randomly assigned to one of two treatment regimens. Group A received midazolam and propofol, while group B received midazolam and pethidine.</p><p><b><i>Results:</i></b> A total of 51 patients were included in the analysis, with 23 and 28 patients in groups A and B, respectively. Adverse events did not significantly differ between the two groups. Additionally, there were no differences in cecal intubation and total procedural times. However, group A demonstrated a lower frequency of postural changes (1.0±0.7 vs. 1.5±0.7, p=0.02) and a reduced rate of manual compression (52.2% vs. 82.1%, p=0.02). There were no significant differences between the two groups in terms of subjective pain or satisfaction.</p><p><b><i>Conclusions:</i></b> Both sedation methods demonstrated similar safety profiles and satisfactory outcomes. The combination of midazolam and propofol showed comfortable insertion by minimizing the need for patient repositioning and manual compression during colonoscopy.</p><p><b>PP-02-348</b></p><p><b>Outcomes of Colorectal Endoscopic Submucosal Dissection According to the Size of Colorectal Neoplasm</b></p><p><b>Hyun-soo Kim</b> and Dong Hyun Kim</p><p><i>Chonnam National University Hospital And Medical School, 42, Jebong-ro, Dong-gu, Gwangju, South Korea</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aim:</i></b> Endoscopic submucosal dissection (ESD) is a valuable technique for treating early colorectal neoplasms. However, there are insufficient data concerning the treatment outcomes in relation to the size of colorectal neoplasms.</p><p><b><i>Patients and Methods:</i></b> The data on ESD for colorectal epithelial neoplasms between January 2015 and December 2020 were retrospectively collected from five tertiary medical centers. Colorectal neoplasms were stratified into groups based on their longitudinal diameter: <20 mm as Group 1, 20–39 mm as Group 2, 40–59 mm as Group 3, and 60 mm or more as Group 4.</p><p><b><i>Results:</i></b> Of the 1,446 patients, 132 patients were in Group 1 (<20 mm), 1,022 in Group 2 (20–39 mm), 249 in Group 3 (40–59 mm), and 43 in Group 4 (≥60 mm). There was an observed trend of increasing age from Group 1 to Group 4, accompanied by a corresponding increase in the Charlson Comorbidity Index. Procedure time also exhibited a gradual increase from Group 1 to Group 4. Similarly, the length of hospital stay tended to increase from Group 1 to Group 4. The predictive model, using restricted cubic spline curves, revealed that as the size of lesion exceeded 30 mm, complete resection steadily decreased, and major complications notably increased.</p><p><b><i>Conclusion:</i></b> As the size of colorectal neoplasms increases, the rate of complete resection decreases and the rate of complications increases.</p><p><b>PP-02-349</b></p><p><b>Unusual cause of upper Gastro-Intestinal Bleeding with gastric and duodenal ischemia: A case report</b></p><p><b>Dalouny Sihalath</b><sup>1</sup>, Sengdao Vannarath<sup>1</sup> and Thitsamay Luangxay<sup>2</sup></p><p><sup>1</sup><i>Mahosot Hospital, Vientiane, Lao People's Democratic Republic;</i> <sup>2</sup><i>National Cancer Center Laos, Vientiane, Lao People's Democratic Republic</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p>Idiopathic Catastrophic Anti-Phospholipid Syndrome (CAPS) is a rare condition with multiple complications to end organ. We report a rare case of CAPS, a 58-year-old Laotian female presented to the emergency department with coffee ground emesis for 2 days and postprandial abdominal fullness for 3 weeks. Physical examination revealed mildly abdominal tenderness. The patient was hemodynamically stable. Laboratory findings were total white blood cell was normal, hemoglobin level of 9.7 g/dL, platelet count of 238,000/cu.mm, normal creatinine level, Prothrombin Time was 18 seconds with an INR level of 1.1.</p><p>Esophagogastroduodenoscopy revealed an edematous purplish discoloration of the mucosa with multiple subepithelial hemorrhages from the proximal gastric body to second part of the duodenum. Abdominal computed tomography showed thickening of gastric and duodenum mucosa walls and decreased enhancement of the wall without pneumatosis. Multiple sites of thrombosis were found at the gastro-duodenal and splenic arteries, superior mesenteric vein thrombosis, splenic vein, and splenic infarction. Further findings included of a C-Reactive protein 95.018 mg/dL, and work-up for hypercoagulable states such as Anticardiolipin antibodies and lupus anticoagulant were normal. Additionally, previous use of oral contraceptive drugs was noted in the patient’s history.</p><p>The final diagnosis was acute gastro-duodenal ischemia due to suspected Idiopathic CAPS and managed with bowel rest, parenteral nutrition, and conservative with Anticoagulant therapy. She gradually improved abdominal pain. Although, CAPS might be rare a medical condition, it can manifest as a bleeding complication and could be fatal. More in-depth study needs to be conduced this syndrome has scarce information.</p><p><b>PP-02-350</b></p><p><b>ASSOCIATION BETWEEN ENDOSCOPY FINDINGS AND THE DEGREE OF INFLAMMATION FROM BIOPSY RESULTS IN GASTRITIS PATIENTS</b></p><p><b>I Komang Wisuda Dwija Putra</b>, I Ketut Mariadi, Gde Somayana and Komang Agus Wira Nugraha</p><p><i>Rsup I.G.N.G Prof Ngoerah, Denpasar, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gastritis is an inflammation of the gastric mucosa which can be acute or chronic. Gastritis may be erosive (superficial, deep, or hemorrhagic) or non-erosive. Endoscopy and stomach tissue biopsy are the most accurate screening method in assessing the severity of gastritis and evaluating the risk of metaplasia. Level of inflammatory cell infiltration and Helicobacter pylori infection proven to be related to increased risk of gastric cancer. By knowing the pattern of severity gastritis in patients, then the level risk of stomach cancer in an area can be predicted. Objectives: evaluating the association between endoscopy findings and inflammation level in gastritis patients.</p><p><b><i>Materials and methods:</i></b> This study uses a retrospective cohort design. Subjects were sampled using consecutive random sampling. Patients with gastritis diagnosis, age 18 - 80 were included in this study. The subject's biopsy material was taken from endoscopy examination. Univariate analysis was carried out to measure the frequency distribution. Bivariate analysis was carried out using chi square test.</p><p><b><i>Results:</i></b> The average age of the subjects was 47.08 ± 14.46 years old with a range of 19 – 79 years old. There was no statistically significant relationship between degree and activity level, H.pylori, atrophic condition, intestinal metaplasia, inflammation level with a p value of 0.353; 0.267; 0.142; 0.512; 0.864. There was also no statistically significant relationship between location and inflammation level with a p value of 0.479.</p><p><b><i>Conclusion:</i></b> There were no significant relationship between endoscopy findings and the degree of inflammation from biopsy results in gastritis patients.</p><p><b>PP-02-351</b></p><p><b>Fecal Bifidobacterium is Associated with Improvement of Liver Fibrosis and Survival in Virus-related Liver Cirrhosis</b></p><p><b>Komang Agus Wira Nugraha</b><sup>1</sup>, I Ketut Mariadi<sup>1</sup>, Gde Somayana<sup>1</sup> and Cokorde Istri Yuliandari Krisnawardani Kumbara<sup>2</sup></p><p><sup>1</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia;</i> <sup>2</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Badung, Indonesia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Imbalance of gut microbiota, has been linked to inflammation and the progression of liver diseases. Investigating the function of the Bifidobacterium in liver cirrhosis holds promise for identifying therapeutic targets that could revolutionize the management of liver cirrhosis and improve the survival. This study aimed to investigated the role of fecal Bifidobacterium in improvement of liver fibrosis and survival.</p><p><b><i>Materials and Methods:</i></b> This prospective cohort study recruited virus-related liver cirrhosis patients in Prof. Dr. I.G.N.G. Ngoerah Hospital from May 2021. All subjects received antiviral therapy for Hepatitis B or C. The fecal Bifidobacterium (CFU/g) was examined using DNA isolation and then categorized as decreased (<0.78 CFU/g) and normal-increased (≥0.78 CFU/g). Liver fibrosis (kpA) was determined using transient elastography. After 6 months of follow up, we evaluated the improvement of liver fibrosis and survival.</p><p><b><i>Results:</i></b> A total of 78 patients were enrolled in this study, and the mean age of subjects were 51.37 years (SD 11.87). The Logistic Regression analysis showed that normal-increased Bifidobacterium was associated with improvement of liver fibrosis (AOR: 11.19, 95% CI: 3.58-34.93, p<0.001). The Kaplan-Meier curve revealed that the median survival of subjects with a normal-increased Bifidobacterium (22 weeks, 95% CI 20.24-23.75) was higher than the subjects with a low Bifidobacterium (12 weeks, 95% CI 9.15-14.84)(p=0.003). A normal-increased Bifidobacterium also had an association with survival (HR: 0.084, 95% CI 0.011-0.66, p=0.019), after adjustment to confounding variables in Cox Regression analysis.</p><p><b><i>Conclusion:</i></b> The normal-increased level of Bifidobacterium is associated with improvement of liver fibrosis dan survival.</p><p><b>PP-03-001</b></p><p><b>Choledochal cyst type ii with cystolithiasis managed with ercp and papillotomy in an adult male</b></p><p><b>Luigi Dan Alit</b> and Ike Minerva</p><p><i>West Visayas State University Medical Center, Iloilo City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Choledochal cyst is a rare biliary tree anomaly, with type 2 comprising only 2% of the different types. It usually presents during childhood and is four times more common in females. Associated complications are cystolithiasis, pancreatitis, and malignant transformation. Simple excision or diverticulectomy is the treatment approach. In this case, work up of an adult male showed choledochal cyst with intracystic stones successfully managed with ERCP and papillotomy.</p><p><b><i>Case description:</i></b> A 33-year-old, male, occasional alcoholic beverage drinker consulted due to one month epigastric pain associated with nausea. No reported fever, jaundice, or palpable mass. Initial work up revealed mild leukocytosis and slightly elevated bilirubin but with normal serum amylase, alkaline phosphatase, and aminotransferases. Initial work up using whole abdomen ultrasound revealed acalculous cholecystitis and non-obstructing right nephrolithiasis. Further imaging studies with abdominal CT scan considered choledochal cyst type II and pancreatic head cyst. MRCP and ERCP confirmed the presence of choledochal cyst with intracystic stones. Papillotomy and biliary stent placement were performed for biliary drainage. Biliary exploration with possible transduodenal excision of cyst was contemplated prior to discharge. On reevaluation after eight months, ERCP showed no choledocholithiases nor distal CBD cyst lithiases.</p><p><b><i>Discussion:</i></b> Choledochal cysts type II is a rare biliary duct anomaly, more so in adults. They may present with nonspecific manifestations which may delay diagnosis, thus, a high index of suspicion is needed. Complications such as cystolithiasis and pancreatitis may necessitate papilla sphincterotomy and/or biliary drainage while awaiting definitive management.</p><p><b>PP-03-002</b></p><p><b>Metastatic Renal Cell Carcinoma Presenting as Gastrointestinal Bleeding: A Case Report</b></p><p><b>Vivien Ang</b> and Porciuncula Vicente II</p><p><i>St. Luke’s Medical Center Quezon City, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Renal Cell Carcinoma (RCC) is infamous for its propensity for widespread metastasis. However, metastasis to the gastrointestinal tract discounting the liver, is extremely rare, comprising only 0.2-0.7% of cases.</p><p><b><i>Case description:</i></b> This is a case of a 44-year-old female who was recently diagnosed with Renal Cell Carcinoma Stage IV (Metastatic to the Lungs, Bones, Lymph Nodes, Adrenals and Ovary by PET-CT scan) presenting with hematochezia. Patient underwent esophagogastroduodenoscopy showing multiple gastric polyps with central ulceration and duodenal ulcers (Forrest Class III), as well as a colonoscopy revealing non-bleeding colon ulcers at the ileocecal valve and ascending colon. Biopsies all revealed ulcerating poorly differentiated carcinoma. Immunohistochemical staining was requested and was positive for Cytokeratin, PAX8, CD10, Vimentin and WT-1, which are compatible with Renal Cell Carcinoma. The patient was managed with blood transfusions and was subsequently started on Lenvatinib.</p><p><b><i>Discussion:</i></b> RCC is a primary renal malignancy which accounts for an estimated 3% of all adult cancers. Metastasis is uncommon in the gastrointestinal tract, with less than 30 cases reported in literature. Colon involvement is rarer compared to the stomach and small bowel- all of which are present in our patient’s case. Symptoms for gastrointestinal tract involvement are mainly gastrointestinal bleeding and iron deficiency anemia. Management options for gastrointestinal metastatic lesions include endoscopic resection, embolization, chemotherapy and immunotherapy, but optimal treatment remains controversial. Our case outlines the importance of careful endoscopic evaluation and histologic examination of RCC patients presenting with gastrointestinal bleeding, since although rare, metastasis should be a consideration.</p><p><b>PP-03-003</b></p><p><b>Hereditary Hemorrhagic Telangiectasia in an Elderly Filipino Female: A Rare Case of Gastrointestinal Bleeding</b></p><p><b>Ferdinand Anzo</b> and Edgardo Bondoc</p><p><i>St. Luke's Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hereditary hemorrhagic telangiectasia (HHT) or Osler-Weber-Rendu syndrome is a rare genetic disorder characterized by small vascular lesions in the oral cavity, lips, fingers, and mucosa of the nasal cavity and gastrointestinal tract. Due to its systemic complications, a multidisciplinary management approach is essential. This case report will discuss a patient with multiple telangiectasia following a clinical diagnosis of HHT, based on the Curaçao diagnostic criteria.</p><p><b><i>Case Presentation:</i></b> This is the case of an 81-year-old Filipino female, known hypertensive, who was admitted due to anemia presenting with epistaxis and melena. Upon physical examination, multiple punctiform telangiectasia on the lips, oral mucosa, and hands. Gastroscopy revealed multiple arteriovenous malformations lacking intervening capillaries in the esophagus, stomach, and duodenum. Argon plasma coagulation was performed. The patient kept NPO while receiving Pantoprazole 40mg IV twice daily and was gradually reintroduced to a diet 24hour post-procedure. On hospital day 4, she was discharged and advised to follow up after one week with a recommendation to undergo repeat gastroscopy.</p><p><b><i>Conclusion:</i></b> Despite not undergoing genetic testing due to a lack of funds, patients present with abnormal blood vessels in several areas of the body (such as the lips, tongue, hands, esophagus, stomach, and duodenum) along with signs of bleeding (epistaxis and melena). This presentation is consistent with HHT as defined by the Curaçao criteria. Additionally, having first-degree relatives with the same condition constitutes the classic triad of the syndrome; however, it is unknown whether the patient is the first in their family to have this syndrome.</p><p><b>PP-03-004</b></p><p><b>Insulinoma Diagnosis: Uncovering a Rare Pancreatic Lesion</b></p><p><b>Ferdinand Anzo</b>, Ronald Vallar and Jan Axel Yusi</p><p><i>St. Luke's Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Insulinoma is an uncommon pancreatic neuroendocrine tumor characterized by disproportionately high levels of insulin or C-peptide during a 72-hour fast-induced hypoglycemia. Diagnostic techniques commonly used to identify pancreatic tumors include CT, MRI, and EUS. Surgical excision are preferred treatments for solitary tumors. This study presents a rare case of insulinoma detected via EUS, despite normal findings on CT and MRI.</p><p><b><i>Case Presentation:</i></b> This is a 74-year-old female with a history of Multinodular toxic goiter, who presented with persistent hypoglycemia, accompanied by loss of consciousness and subsequent recovery following intravenous glucose administration. Work-up revealed elevated C peptide (8.28 ng/mL) and fasting insulin (281.90 uU/mL). A whole abdominal CT scan showed unremarkable findings. A subsequent abdomen MRI revealed a small hepatic and renal cyst, with the pancreas appearing unremarkable. Despite these findings, due to the persistence of hypoglycemia, an EUS was performed, revealing a 3.5 mm hypoechoic to almost anechoic well-defined lesion at the pancreatic body. FNAB confirmed a round cell neoplasm. Immunohistochemical staining showed positivity for Synaptophysin, Chromogranin, with a Ki67 index of 1.3% and retained nuclear expression of SMAD 4. The patient subsequently underwent pancreatic enucleation, which confirmed a neuroendocrine tumor. Following surgery, the patient has reported no further episodes of hypoglycemia.</p><p><b><i>Conclusion:</i></b> In summary, for suspected cases of insulinoma, imaging techniques like CT and MRI are initial steps, but invasive modalities such as EUS are notably superior for localizing lesion. Laparoscopic enucleation is the preferred surgical technique, ensuring complete removal of the tumor capsule to prevent recurrence.</p><p><b>PP-03-005</b></p><p><b>Neuroendocrine Tumor presenting as cystic lesion in the Liver and Pancreas</b></p><p><b>Ferdinand Anzo</b>, Juliet Cervantes and Jessika Iza Chua</p><p><i>St. Luke's Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Neuroendocrine tumors are indeed uncommon compared to other types of tumors, but they can occur in various organs, including the pancreas and liver. While solid masses are predominantly common presentations with higher incidence in males, cystic forms do occur, though less frequently. Neuroendocrine tumors of the pancreas and liver to be discussed underscores the challenge in diagnosing and determining the specific location of tumor origin involving an adult female presenting with abdominal pain.</p><p><b><i>Case Presentation:</i></b> A 47-year-old female presented with intermittent right upper quadrant abdominal pain initially managed as cholecystolithiasis. Medications provided partial relief, leading to hepatobiliary ultrasound revealing an enlarged right liver lobe with a 19.9 x 18.3 x 18.4 cm complex mass and another 12.2 × 13.9 × 11.1 cm mass in the mid upper abdomen. Laboratory tests showed normal liver function but elevated lipase (3x) and CA19-9 (55.36). CT scans revealed hepatomegaly with cirrhotic changes and multiple peripherally enhancing hypodense lesions in the liver, and an atrophic pancreas with a large, complex cyst. Aspiration and drainage were performed, with negative cultures and cytology. She underwent exploratory laparotomy and wedge resection confirming a Grade 2 Well-Differentiated Neuroendocrine Tumor</p><p><b><i>Conclusion:</i></b> Cystic neuroendocrine tumors of the pancreas and liver are rare and pose diagnostic challenges, often overlooked due to nonspecific symptoms, leading to delayed diagnosis and treatment. Prognosis varies based on factors such as tumor grade, stage at diagnosis, presence of metastasis, and hormone secretion status (functional vs. non-functional).</p><p><b>PP-03-006</b></p><p><b>Clinical Outcomes of Achalasia after Per-oral Endoscopic Myotomy from a Tertiary Hospital in the Philippines</b></p><p><b>Ferdinand Anzo</b> and Marc Julius Navarro</p><p><i>St. Luke's Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Achalasia is an uncommon neurogenic esophageal motility disorder characterized by incomplete relaxation of the lower esophageal sphincter, coupled with the absence of organized peristalsis. POEM is a hybrid technique for treating achalasia and preferred and current endoscopic treatment of choice due to lower complication rate compared to laparoscopic Heller myotomy. Since POEM has only been introduced to the Philippines recently, there are few centers who offer this procedure. So in this study, we will focus on the patients who underwent POEM and their clinical outcomes.</p><p><b><i>Case Presentation:</i></b> Each of the five female patients sought consultation for dysphagia associated with typical clinical manifestation of esophageal motility disorder. All patients underwent EGD and high resolution esophageal manometry (HREM) as part of the pre evaluation of POEM. It revealed retained food particles in the esophageal lumen, dilated esophagus with narrowing of the esophagogastric junction (EGJ) with puckered appearance and type 1 achalasia in HREM. A barium esophagogram was also done to all patients with consistent findings of achalasia. Pre-POEM and Post-POEM Eckardt score with reflux symptoms were evaluated using GERDQ system with positive outcomes. All patients who underwent repeat EGD noted a good myotomy site and the EG junction. No complications were noted for all patients after POEM.</p><p><b><i>Conclusion:</i></b> Achalasia is a rare motility condition. There is a good clinical outcome of patients diagnosed with achalasia after POEM in spite of different variables. All five subjects showed symptoms improvement and weight gain underscoring POEM’s important role in the management of achalasia.</p><p><b>PP-03-007</b></p><p><b>The Effectiveness of Left Lateral Decubitus Sleeping Position in Gastroesophageal Reflux Disease (GERD): An EBCR</b></p><p><b>Fhathia Avisha</b> and Rima Karlina</p><p><i>Department Of Internal Medicine, University Of Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> GERD is a disorder in which gastric contents experience repeated reflux into the esophagus, causing disturbing symptoms and complications.</p><p><b><i>Objective:</i></b> To determine the effectiveness of non-pharmacological therapy for left lateral decubitus (LLD) and right lateral decubitus (RLD) and supine sleeping positions on reflux symptoms in adult patients with GERD.</p><p><b><i>Methods:</i></b> A literature search was conducted on five databases, namely Pubmed, Cochrane, Scopus, and Sciencedirect on February 19, 2024 using the keywords “Gastroesophageal Reflux Disease,” and “Sleep Position” or “Left Lateral Decubitus” or “Right lateral Decubitus or Supine AND nocturnal reflux symptoms or Symptoms”. There were seven studies on Pubmed, no study on Cochrane, one study on Scopus, and eighteen studies on Sciencedirect. The articles found were screened based on the inclusion and exclusion criteria.</p><p><b><i>Results:</i></b> There were significant differences in acid exposure time (AET) and acid clearance time (ACT) in patients with LLD sleep position compared to RLD and supine sleep position. Two non-randomized studies showed decreased AET and ACT in LLD sleep position compared to RLD (mean difference [MD] -2,03 [95% CI: -3,62 to -0,45]; -81,84 [95% CI: - 127,48 to -36,20],) and supine position (MD -2,71 [95% CI: -4,34 to -1,09]; -74,47 [95% CI: - 116,26 to -32,69], respectively).</p><p><b><i>Conclusion:</i></b> LLD sleeping position significantly reduced acid exposure time and acid clearance time among patients with GERD compared to RLD and supine sleeping positions. LLD sleep position is recommended for GERD patient to reduce reflux symptoms.</p><p><b><i>Keywords:</i></b> Sleep positions, Left Lateral Decubitus, Nocturnal Gastroesophageal Reflux.</p><p><b>PP-03-008</b></p><p><b>Recurrent severe anaemia related to rare oesophageal and gastric lymphangioma treated with haemostatic radiation therapy</b></p><p><b>Muhammad Khifzhon Azwar</b>, Amalia Nur Pratiwi, Leonard Nainggolan, Amanda Pitarini Utari and Saskia Aziza Nursyirwan</p><p><i>Faculty Of Medicine, Universitas Indonesia - Department Of Internal Medicine, Cipto Mangunkusumo Hospital, Jakarta Central Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Review of cases published in English in electronic databases showed only 30 cases of oesophageal lymphangioma and 24 cases of gastric lymphangioma ever reported. We aimed to report successful management of oesophageal and gastric lymphangioma case presenting with recurrent severe microcytic anaemia due to gastrointestinal bleeding and iron deficiency.</p><p><b><i>Case Description:</i></b> A 21-year-old lady came with chief complaint of worsening fatigue since 3 days before admission. Vomiting of fresh blood preceded the worsening of fatigue, accompanied by epigastric pain and passing black stools. The complaints have recurred since 3 years before admission. We observed malnutrition and unilateral preaxial polydactyly of the upper extremity. Full blood count on admission often showed haemoglobin levels between 2 and 4 g/dL. Fatigue episodes improved with packed cells transfusions. Oesophagogastroduodenoscopy showed proliferative mass starting from distal oesophagus, extending to oesophagogastric junction and gastric cardia. Pathological examinations suggested gastric polyp, as well as gastric and oesophageal lymphangioma. Combination of dietary modification per nasogastric tube, packed cells transfusion, iron repletion, and symptomatic treatments did not result in lasting improvement. Patient received 10 fractions of 2 Gy haemostatic intensity-modulated radiation therapy (IMRT) and subsequently achieved two-month clinical, radiological, and laboratory-based improvements.</p><p><b><i>Discussion:</i></b> Anaemia and gastrointestinal bleeding were considered rare clinical presentations of oesophageal and gastric lymphangioma. There may be genetic component contributing to the occurrence of lymphangioma in our case report patient. Haemostatic IMRT is proven to be safe and effective for this case.</p><p><b>PP-03-009</b></p><p><b>Hepatitis A presenting with Acute Liver Failure and Severe Acute Pancreatitis: A rare case report</b></p><p><b>Rajat Bansal</b><sup>1</sup>, Hitendra Garg<sup>1</sup>, Mohammad Irtaza<sup>1</sup>, Arun Kumar<sup>2</sup>, Sudeep Khanna<sup>1</sup> and Neerav Goyal<sup>2</sup></p><p><sup>1</sup><i>Department of Gastroenterology and Hepatology, Indraprastha Apollo Hospital, New Delhi, India;</i> <sup>2</sup><i>Department of Liver Transplant, Hepatobiliary and Pancreatic Surgery, Indraprastha Apollo Hospital, New Delhi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Acute liver failure (ALF) and severe acute pancreatitis (SAP) are rare but serious complications of hepatitis A virus (HAV) infection, particularly in adolescents. This report presents a case of a young male diagnosed with both ALF and SAP secondary to acute HAV infection.</p><p><b><i>Case Report:</i></b> A 17-year-old male presented with fever for three days followed by severe abdominal pain, vomiting, jaundice, and altered mental status for two days. Investigations showed elevated liver enzymes, hyperbilirubinemia, coagulopathy, and raised ammonia levels. Serum lipase, amylase were markedly elevated, confirming acute pancreatitis. HAV IgM antibodies were positive. Imaging showed hepatomegaly without biliary obstruction and diffuse pancreatic inflammation with peripancreatic collection. He had encephalopathy and respiratory failure and was intubated and managed in ICU with supportive therapy, N-acetyl-cysteine, and CRRT. Due to worsening sepsis, percutaneous drainage of peripancreatic collection was done. He later underwent retroperitoneal debridement and necrosectomy for infected peripancreatic collections. He gradually improved and was discharged in stable condition.</p><p><b><i>Discussion:</i></b> Hepatitis A typically causes self-limiting illness, but rarely, it can lead to ALF and SAP. ALF occurs in less than 1% HAV cases. Acute pancreatitis has been reported to occur mostly without fulminant hepatitis (94%) in HAV and is severe in 9% cases. The pathophysiology is not fully understood. HAV is proposed to cause ALF from an overwhelming immune-mediated response leading to massive hepatocyte necrosis and pancreatitis due to immune-mediated mechanisms, direct viral injury, and ampullary edema causing pancreatic fluid outflow obstruction. Early recognition and supportive management are essential for favorable outcome.</p><p><b>PP-03-010</b></p><p><b>Pediatric recurrent acute pancreatitis with choledochal cyst, pancreatobiliary maljunction and ansa pancreatica: A Case Report</b></p><p><b>Rajat Bansal</b>, Mohammad Irtaza, Anupam Sibal and Hitendra Garg</p><p><i>Indraprastha Apollo Hospital, New Delhi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Recurrent acute pancreatitis (RAP) in children is rare and often poses a diagnostic challenge. We report a pediatric case of RAP with a triple whammy of pancreaticobiliary anomalies: choledochal cyst, pancreaticobiliary maljunction (PBM), and ansa pancreatica.</p><p><b><i>Case Description:</i></b> A 11-year-old female presented with acute abdominal pain and a history of recurrent pancreatitis since age 4. Initial MRCP revealed choledochal cyst, cholelithiasis and choledocholithiasis. She had undergone ERCP, CBD clearance, and later, Roux-en-Y hepaticojejunostomy with cholecystectomy. Despite surgery, she experienced recurrent episodes of pancreatitis requiring hospitalizations. This episode of pancreatitis was mild and managed conservatively. MRCP showed dilated remnant CBD with intraluminal defects and peripancreatic inflammation in head of pancreas. ERCP revealed a dilated residual CBD stump with small filling defects and a long common pancreaticobiliary channel, and balloon sweeps retrieved small calculi and sludge. Pancreatic duct cannulation via minor papilla revealed an ansa pancreatica loop. Minor papilla sphincterotomy was done but pancreatic stent placement was not possible. The patient had no further pain episodes on follow-up.</p><p><b><i>Discussion:</i></b> In pediatric patients, anatomical anomalies should be considered as a cause of RAP. PBM leads to continuous reflux between pancreatic juice and bile, causing pancreatitis and is often associated with choledochal cyst. Ansa pancreatica, a rare anatomic variant, is characterized by a curved communicating duct between the ventral and dorsal ducts. It has been reported with choledochal cysts and can predispose to pancreatitis due to poor pancreatic duct drainage. Treatment typically involves supportive measures, with ERCP and sphincterotomy considered in refractory cases.</p><p><b>PP-03-011</b></p><p><b>Insidious colitis: amebiasis</b></p><p><b>Dmitriy Baranov</b> and Anna Poddymova and Evgeniy Solonitsyn</p><p><i>Almazov Nmrc, Saint-Petersburg, Russia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Currently, inflammatory bowel diseases (IBD) are widespread and well-known, but another pathology may be hiding under this mask.</p><p><b><i>Materials and methods:</i></b> We present the case of a white 61-year-old man who, against the background of complete well-being, had an episode of abdominal pain, rectal bleeding, without fever and features of an epidemiological history.</p><p>Colonoscopy with colon biopsy was performed. There is ulcerative lesion in the dome of the cecum, EGDS – without features. Laboratory tests for bacterial, viral and parasitic diseases are negative. ANCA, ASCA is the norm. Anemia of mild degree (Gb 122 g/l). Morphology was performed in three different laboratories, only one revealed amoebas. The patient received a course of Metronidazole for 14 days, was sent to an infectious disease specialist: \"amoebiasis\" was excluded by the doctor. However, inflammatory changes and anemia persisted. On repeated clonoscopies– ulceration is preserved. The diagnosis was made – Crohn's disease of the colon, treatment was started (Prednisone, Azathioprine) – without effect.</p><p>A year later, the deterioration of the condition, laboratory parameters. Hospitalization, diagnosis – myeloma. Colonoscopy, morphology: signs of amoebiasis. 5-day course of Paromomycin</p><p><b><i>Results:</i></b> At the control colonoscopy: no ulcers, morphology – no amoebas.</p><p>The patient is currently being treated for myeloma.</p><p><b><i>Conclusion:</i></b> Often a completely different disease is hidden under the mask of one disease, and doctors can make mistakes. In diagnostics, it is important not only to perform analyses, but also to double-check them, despite the opinions of other specialists.</p><p><b>PP-03-012</b></p><p><b>A successful ERCP outcome in a pregnant patient with acute choledocholithiasis in severe cholangitis</b></p><p><b>Bautista Carmelo III</b> and Caesar Noel Palaganas and Marie Ellaine Velasquez and Jonathan Viernes</p><p><i>Baguio General Hospital Medical Center, Baguio City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gallstone-related disease occurs in <1% in pregnancy. This rare non-obstetric emergency may threaten the fetus and mother’s life and requires a multidisciplinary approach. In this report, we aimed to describe clinical and morphological features of acute cholangitis in our pregnant patient and discussed appropriate management.</p><p><b><i>Case description:</i></b> This case is a pregnant 28-year-old Filipino female at 29-weeks-age-of-gestation who presented with jaundice and increasing epigastric pain. Ultrasound showed dilated CBD at 1.4cm with a 1.7cm stone in its distal segment. Physical exam showed a gravid, tympanitic and soft abdomen with a positive Murphy’s sign. A diagnosis of obstructive jaundice secondary to choledocholithiasis in severe cholangitis was made. Pertinent laboratories revealed leukocytosis, transaminitis and hyperbilirubinemia. ERCP showed multiple choledocholithiasis and acute purulent cholangitis. Cholangiogram revealed dilated CBD and CHD, with multiple filling defects in the distal CBD, the largest ~18mm. Bile fluid showed growth of Stenotrophomonas maltophilia, TB-PCR was negative. Lastly, a long biliary stent was inserted showing good egress of fluid. No complications were encountered during the procedure.</p><p><b><i>Discussion:</i></b> During pregnancy, acute lithiasis cholangitis is rare and present with classic Charcot’s triad. Among imaging studies, abdominal ultrasound remains the first-line examination.Using more specific modalities i.e. CT and MRI may be limited and crucial in pregnancy. Hence, a multidisciplinary approach is necessary. With this, ERCP with sphincterotomy and stone extraction in acute cholangitis among pregnant women is the better and safer therapeutic option. The approach may be challenging for the gastroenterologist but early recognition without delay of complications is necessary.</p><p><b>PP-03-013</b></p><p><b>Esophageal Stent Insertion: A Bridge to the Treatment of Tuberculous Bronchoesophageal Fistula</b></p><p><b>Jan Bendric Borbe</b>, Mark Anthony De Lusong and Carlo Jay Dejelo</p><p><i>Philippine General Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Infection and trauma are among the leading benign causes of bronchoesophageal fistulas. Conventionally, bronchoesopahgeal fistulas are treated by surgical resection of the fistulous tract. However, infectious causes, including tuberculosis, have been described in literature to resolve with appropriate anti-microbial treatment. This case describes a patient who underwent esophageal stenting while undergoing anti-Koch’s treatment.</p><p><b><i>Case Description:</i></b> The patient is a 37/M, known case of HIV WHO Clinical Stage IV on anti-retroviral agents. He presented with a 1 month history of dysphagia with post-prandial coughing episodes prompting admission. CT scan done on admission showed a bronchoesophageal fistula at the left main bronchus. An upper endoscopy was subsequently performed. An ulcer with a fistulous opening at 31cm from the oral incisors was seen. Segments of the bronchial tree can be visualized at the distal portion of the fistulous tract. Multiple biopsy samples taken over the ulcer showed granulation tissue with marked acute on chronic inflammation. Samples sent for TB GeneXpert came back positive. A fully covered 22x100mm esophageal stent was placed over the fistula. Upon completion of anti-Koch’s treatment, a repeat upper endoscopy was done to remove the esopahgeal stent. The previously noted defect was seen to have re-epithelialized.</p><p><b><i>Discussion:</i></b> The case highlights the utility of esophageal stenting in patients with bronchoesophageal fistulas with a tuberculous etiology. It provides an opportunity to allow spontaneous closure of the fistula while the patient is receiving treatment. Furthermore, esophageal stenting provides an enteral access to improve nutritional status and quality of life.</p><p><b>PP-03-014</b></p><p><b>Treatment of advanced synchronous hepatocellular carcinoma and rectal cancer: A successful case</b></p><p><b>Quang Loc Bui</b><sup>1</sup>, DucHuy Tran<sup>2</sup>, MauThai Nguyen<sup>3</sup>, ThangNgoc Nguyen<sup>4</sup> and Akiko Tomonari<sup>1</sup></p><p><sup>1</sup><i>Hanoi French Hospital, Hanoï, Viet Nam;</i> <sup>2</sup><i>Bach Mai Hospital, Hanoi, Viet Nam;</i> <sup>3</sup><i>VietNam National Cancer Hospital, Hanoï, Vi ệ t Nam;</i> <sup>4</sup><i>Central Military Hospital 108, Hanoï, Vi ệ t Nam</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> The frequency of synchronous multiple primary cancers is rare, from 2 to 17% in literature.</p><p>Herein, we report the successful treatment of synchronous hepatocellular carcinoma (HCC) and rectal cancer (RC).</p><p><b><i>Case Report:</i></b> A 72 years old male, with a history of two small bowel resections for firearm injuries and a cured hepatitis C, presented with multiple HCC’s. The largest lesion was 130x77 mm with portal vein tumor thrombus. AFP was 852.6 ng/ml. Subsequently, an adenocarcinoma was found 5 cm from the anal verge, cT3bN0. CEA was unelevated.</p><p>HCC was prioritized. Atezolizumab and bevacizumab (AB) were chosen for the first line treatment. At 4 months after initiating AB, HCC showed partial response with a decrease of the largest lesion to 56x67 mm and dissapearances of the smaller lesions. AFP was decreased to 6 ng/ml.</p><p>Following this course, radio-chemotherapy for rectal cancer with CAP-50 protocol was started with concomitant atezolizumab monotherapy. Resection for the rectal cancer was not performed due to the past history of multiple abdominal surgeries. AB was restarted after radiotherapy, which continued to show an excellent response with mRECIST CR for HCC and PR for RC.</p><p><b><i>Discussion:</i></b> Synchronous double advanced cancers are rare and can be challenging for prioritizing the target and choosing the optimal agents.</p><p>In this case, using bevacizumab concomitant with radiation increased the risk of fistula formation; thus, we decided to use atezolizumab for HCC and CAP-50 protocol for rectal cancer. Conclusively, we were able to achieve sufficient response with no major adverse events.</p><p><b>PP-03-015</b></p><p><b>A rare case of Dysphagia</b></p><p>Nazish Butt, <b>Ghulam Mohiuddin</b> and Gulzar Khan</p><p><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Esophageal leiomyomas are the most common benign tumors of the esophagus, being pedunculated polyp presentation is very rare. A 65-year-old female having symptoms of troublesome dysphagia was found, incidentally on Esophagogastroduodenoscopy (EGD) as pedunculated polyp of approximately size 3 ×1.7×1.5 cm in size covered by normal looking mucosa. The polypoid lesion was then removed successfully with hot polypectomy snare. The histopathological diagnoses of the polyp turned out as leiomyoma. Patient symptoms were relieved after successful removal.</p><p><b>PP-03-016</b></p><p><b>A large liver mass turned out to be a rare tumor of Liver</b></p><p>Nazish Butt, <b>Ghulam Mohiuddin</b> and Gulzar Khan</p><p><i>Jinnah Postgraduate Medical Centre, Karachi, Pakistan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Follicular Dendritic Cell sarcoma (FDS) of the liver is a rare entity. It mostly presents in the lymph nodes, extra lymph nodal presentation is very rare A 40 -year-old male having no known comorbidities presented to us with complaints of abdominal pain and abdominal distension for the last 2 months, Triphasic CT scan abdomen showed large irregular mass of approximately size of 17.7 × 15.7×15.9 cm in the right lobe of the Liver with thrombus in the Portal Vein, splenomegaly and mild abdominal ascites representing atypical Hepatoma , the biopsy was done which was consistent with Follicular Dendritic Cell Sarcoma (FDS), a very rare tumor.</p><p><b><i>Keywords:</i></b> Follicular Dendritic Cell sarcoma, abdominal pain, abdominal distension, Portal Vein Thrombus, Atypical Hepatoma</p><p><b>PP-03-017</b></p><p><b>Conversion therapy with Durvalumab and GemCis in advanced intrahepatic cholangiocarcinoma achieved an extremely-long recurrence-free period</b></p><p><b>Yajuan Cao</b>, Jin Peng and Decai Yu</p><p><i>Division of Hepatobiliary and Transplantation Surgery, Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Biliary tract cancer (BTC) is an aggressively invasive malignancy often diagnosed at an advanced stage, precluding surgical intervention and conferring a dire prognosis. The TOPAZ-1 study demonstrated that the combination of durvalumab (D) plus gemcitabine and cisplatin (GC) yielded significant survival benefits. We present a case of stage IV intrahepatic cholangiocarcinoma (ICC) that underwent successful conversion with D plus GC, achieving a recurrence-free status 19 months post-surgery.</p><p><b><i>Case Description:</i></b> A 51-year-old male was diagnosed with stage IV ICC, with left supraclavicular lymph nodes metastasis. Following four cycles of GC and two doses of durvalumab, the target lesions shrank by 30% (RECIST 1.1), with the disappearance of distant lymph nodes and a notable reduction in regional lymph nodes, achieving partial remission (PR). The patient was subsequently downstaged and underwent laparoscopic radical liver resection and regional lymph nodes dissection. Postoperative pathology revealed ICC with tumor necrosis rate of approximately 90%. Adjuvant therapy with durvalumab was administered for six months post-surgery. The patient has remained disease-free for over 19 months.</p><p><b><i>Discussion:</i></b> Radical surgery remains the cornerstone of treatment for BTC, offering the best chance for long-term survival. The patient had high expression of PD-L1. The addition of PD-L1 inhibitor to the previous standard treatment (GC) had significant therapeutic effects and provided the patient with the opportunity for radical surgery. Continuing adjuvant therapy post-surgery has prolonged his disease-free interval to 19 months. The D+GC regimen presents a promising alternative for conversion therapy in advanced BTC patients, underscoring further exploration in extensive clinical trials.</p><p><b>PP-03-018</b></p><p><b>Eosinophilic gastrointestinal disease involving multiple segments of the gi tract with ascites: a case report</b></p><p><b>Jobel Feliz Castillo-Corañez</b> and Eulenia Nolasco</p><p><i>Manila Doctors Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Eosinophilic gastrointestinal disease (EGID) is a rare inflammatory condition characterized by eosinophilic infiltration of specific segments of the gastrointestinal tract, leading to diverse symptoms. This case report discusses a 53-year-old female with an uncommon EGID presentation involving multiple segments from both the upper and lower GI tract. She also presented with ascites, another rare manifestation.</p><p><b><i>Case description:</i></b> The patient presented with reflux, heartburn, bloatedness, epigastric pain, vomiting, and loose watery stools. Symptoms persisted despite initial treatment for GERD and infectious diarrhea. Investigations revealed significant eosinophilia in peripheral blood and was also noted to have eosinophilic ascites. Endoscopy and colonoscopy were done, with multiple biopsies, and confirmed eosinophilic infiltration in the esophagus, stomach, small intestine, and colon. The patient was treated with montelukast and corticosteroids, which resulted in rapid and marked improvement of all her symptoms.</p><p><b><i>Discussion:</i></b> EGID’s clinical manifestation is dictated by the specific segment (esophagus, stomach, intestine, or colon) and the layer of the GI wall (mucosa, muscularis, serosa) involved. This case underscores the rare and complex presentation of EGID in our patient involving multiple GI segments consistent with her symptoms. Likewise, the presence of ascites suggests deep eosinophilic infiltration to the serosal layer, which is the least common layer affected. This case contributes knowledge to the literature that despite multiple segments and bowel wall layer involvement, the condition is very responsive to therapy, emphasizing the importance of awareness of this condition among clinicians for early diagnosis and appropriate treatment.</p><p><b>PP-03-019</b></p><p><b>An Extremely Rare Case of Secondary Aorto-enteric Fistula in a Filipino Patient with Herald Bleed</b></p><p><b>Alyssa Chan</b></p><p><i>St Luke's Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Secondary aortoenteric fistula (SAEF) is a fistulous communication between the duodenum and the aorta. SAEF is a rare yet lethal complication of abdominal aortic reconstruction which arises months to years after aortic surgery. Herald bleeding refers to a bleeding episode with abdominal pain that occurs before a severe hemorrhage. This is a case of SAEF in a young male who presented with massive hematochezia.</p><p><b><i>Clinical Presentation:</i></b> 32-year-old male with past medical history of abdominal aortic aneurysm repair presented with herald bleeding of 3 weeks’ duration. Endoscopy showed unremarkable results. Examination revealed tachycardia, generalized pallor and epigastric tenderness. Tests showed hemoglobin of 7.9 mg/dL, platelets of 251,000 /μL, and INR of 1.26.</p><p><b><i>Results:</i></b> Tagged RBC scan was negative. Antegrade Push Enteroscopy showed an SAEF with eroding graft at segment D3-D4. Exploratory laparotomy revealed the secondary aortoduodenal fistula at D3 segment with multiple pockets of necrotic tissue and densely adherent to the native aorta and graft. The patient underwent SAEF repair and post-operative course was complicated by sepsis and DIC and eventually went into arrest on his fourth week.</p><p><b><i>Significance:</i></b> This is the first case of SAEF documented at our institution, and possibly within the nation as well. It is extremely rare with an estimated annual incidence of 0.036–1.6%.</p><p><b><i>Recommendations:</i></b> Every patient presenting with UGIB with a history of aortic graft surgery should be presumed to have an aortoenteric fistula until proven otherwise. All patients should undergo early endoscopy as prompt surgical intervention is the key to survival among these patients.</p><p><b>PP-03-020</b></p><p><b>Case Report: AI-Assisted Capsule Endoscopy Rapidly Detecting Obscure Gastrointestinal Bleeding in High-Risk Cardiac Patients</b></p><p><b>Wah Loong Chan</b>, Sivaraj Xaviar, Esther Jara Edmund Nyipa and Stanley Khoo</p><p><i>University Of Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> This report details our experience using Artificial Intelligence (AI)-assisted capsule endoscopy in diagnosing obscure gastrointestinal bleeding (GIB) in a high-risk patient with a recent non-ST elevation myocardial infarction (NSTEMI).</p><p><b><i>Case description:</i></b> A 79-year-old man with coronary artery disease presented with NSTEMI and underwent percutaneous coronary intervention (PCI). He was initiated on antiplatelet therapy but he developed melena and his haemoglobin dropped until 6.4g/dL. Conventional diagnostic methods, including upper endoscopy, colonoscopy, and CT angiography, failed to locate the bleeding source. His hemoglobin continued to decline by 1 g/dL daily despite transfusions. We employed AI-assisted capsule endoscopy (NaviCam® SB System with ProScan™ AI) to identify the cause of his persistent anemia.</p><p>Initial colonoscopy revealed clots and blood suggesting small bowel bleeding, but further examinations, including a second colonoscopy, showed no active bleeding. AI-assisted capsule endoscopy quickly detected mid-jejunal bleeding within minutes upon initiation of its ProScan™ AI feature, displaying a much faster analysis than the manual review methods. This led to surgical intervention, where an exploratory laparotomy and on-table enteroscopy confirmed an arteriovenous malformation 70 cm beyond the duodenojejunal flexure, which was successfully resected.</p><p><b><i>Discussion:</i></b> Our experience highlights the transformative potential of AI-assisted capsule endoscopy in managing obscure GIB. The technology’s quick and precise detection was crucial for timely surgical intervention, enabling prompt management and continuation of essential antiplatelet therapy in this high-risk patient.</p><p><b>PP-03-021</b></p><p><b>A case of ulcerative colitis associated with Hypersensitivity Ectodermal Dysplasia</b></p><p><b>Tsubomi Cho</b> and Shingo Tsuda</p><p><i>Tokai University, Hachioji, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Ectodermal dysplasia is a genetic disorder that causes dysplasia of tissues derived from the ectoderm, such as skin appendages, teeth, and nails. Among them, anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) is caused by mutations in the gene encoding nuclear factor-κB essential modulator protein (NEMO) and abnormalities of the inhibitor of NFκB α (IKBA) gene have been reported.</p><p><b><i>Case report:</i></b> The patient reported here is an 18-year-old man with an EDA-ID–like phenotype, including ectodermal dysplasia with sparse hair (hypotrichosis), abnormal conical teeth, and hypo hidrosis. He had recurrent hemorrhoids fistula since childhood. In 20XX, he visited hospital due to fever, diarrhea, and bloody stools. Colonoscopy was performed and referred to our department because ulcerative colitis was suspected. In colonoscopy, revealed edema, redness, inflammation and pus are appearance in the sigmoid colon and rectum. Pathology showed diffuse inflammatory cell infiltration, crypt abscess, goblet cell depletion, and abnormal glandular arrangement. The diagnosis of ulcerative colitis was made.</p><p>Upper gastrointestinal endoscopy and small bowel angiography were performed and no abnormalities were found. He was treated with mesalazine and prednisolone which provided symptom improvements and discharged in Day28.</p><p><b><i>Discussion:</i></b> In this case, his father and aunt had the same disease, so NEMO gene abnormality (X-linked recessive heredity) is assumed to be present. This type of EDA-ID is estimated to be 1 in 250,000 individuals. Patients who have NEMO gene abnormality with ulcerative colitis is rare condition.</p><p><b>PP-03-022</b></p><p><b>Gastrointestinal Tuberculosis Appearing as a Colon Mass</b></p><p>Franz Jeryl Liangco, <b>Nicole Allyson Chua</b>, Gina Tecson and Kenji Sato</p><p><i>Cardinal Santos Medical Center, San Juan City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal tuberculosis (GI TB) accounts for approximately 1% to 3% of all the tuberculosis cases worldwide. Early diagnosis remains difficult, due to the nonspecific clinical presentation of TB, which can mimic other gastrointestinal diseases and may vary from an acute to a chronic abdomen in areas endemic for TB. A delay in diagnosis and treatment can result in significant morbidity and mortality.</p><p><b><i>Case Description:</i></b> This is a case of a Filipino male in his early 50s, with no known comorbidities, with 1-year history of 11-kg weight loss, and a strong family history of colon cancer, who underwent screening colonoscopy revealing an ulcerating, friable, granular mass at the ascending colon approximately 1-1.5cm in widest diameter. Magnified chromoendoscopy suggests non-neoplastic features. Multiple biopsies taken and showed negative TBGeneXpert as well as absence of acid-fast bacilli but showed chronic granulomatous inflammation with multinucleated Langhans type giant cells as well as acute-on-chronic colitis with mucosal erosion and architectural distortion; positive for crypt abscess. Patient was then started on empiric anti-tuberculosis treatment. The patient completed a 6-month course of the anti-tuberculosis regimen with repeat colonoscopy revealing mucosal healing of the intestinal lesion, as well as weight gain of 2-kg post-treatment.</p><p><b><i>Discussion:</i></b> Gastrointestinal tuberculosis poses a diagnostic challenge as it typically presents with non-specific clinical, radiologic and endoscopic features. High index of clinical suspicion and appropriate use of investigative modalities, can aid in early recognition, diagnosis and prompt treatment thereby reducing morbidity and mortality.</p><p><b>PP-03-023</b></p><p><b>Gastrointestinal Tuberculosis Mimicking Ulcerative Colitis in a Young Filipino Female</b></p><p><b>Vernon Chuabio</b></p><p><i>Philippine General Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal tuberculosis (TB) can mimic numerous conditions like inflammatory bowel disease (IBD). A usual dilemma is differentiating between gastrointestinal TB and Crohn disease since both commonly involve the ileocecal region, unlike ulcerative colitis (UC). This case describes a patient initially assessed to have UC but eventually diagnosed with gastrointestinal TB.</p><p><b><i>Case Description:</i></b> A 23-year-old Filipino female with no known comorbidities and unremarkable family history presented with 1-month watery diarrhea, rectal pain, and weight loss. She had no fever, abdominal pain, or hematochezia. On physical examination, she had bitemporal wasting, pink palpebral conjunctivae, and soft nontender abdomen. A firm, blood-tinged, nodular mass was noted on digital rectal examination. Contrast-enhanced abdominal CT scan showed a 6 x 6.2-cm circumferential, enhancing rectal mass. Colonoscopy revealed a 10 cm-long friable mass-like ulcerating lesion in the rectum, involving the anal verge. Transversely oriented clean-based ulcers 3-4 cm-long were seen in the right colonic side and sigmoid. The remaining colonic segments and terminal ileum were normal-looking, while the ileocecal valve was competent and also normal-looking. Mesalazine was started given an overall presumptive diagnosis of UC. Biopsies finally showed chronic granulomatous inflammation with caseation necrosis. Sputum tests also microbiologically confirmed TB. With the revised TB diagnosis, mesalazine was shifted to anti-tuberculosis therapy.</p><p><b><i>Discussion:</i></b> A lack of ileocecal involvement does not rule out gastrointestinal TB, which may still be considered in left-sided colonic disease that can mimic UC, especially in TB-endemic regions. Correlation between clinical, endoscopic, and histologic findings is crucial for proper diagnosis and treatment.</p><p><b>PP-03-024</b></p><p><b>Varied Presentations of Adult Gastrointestinal Tuberculosis: A Case-series Of 5 Filipino Patients</b></p><p><b>John Derek Clutario</b></p><p><i>Makati Medical Center, Pasig, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Tuberculosis (TB) showed an increasing rate from 2020 to 2022. Among the cases of TB, 15-25% was estimated to be extrapulmonary. Among these, gastrointestinal tuberculosis, the most common form of abdominal TB, has shown a rising trend in recent years. It poses significant diagnostic and therapeutic challenges due to the non-specific and varied nature of its symptoms, limited diagnostic test accuracy, impaired drug absorption with GI involvement, and drug resistance. Timely diagnosis and treatment are essential to prevent severe morbidity, prolonged hospitalization, and the need for surgical intervention.</p><p><b><i>Case description:</i></b> This is a case series of 5 patients showing the wide array of presentations of GI TB like severe abdominal pain, dysphagia, gastrointestinal bleeding, gastric outlet obstruction and even a palpable mass.</p><p><b><i>Case 1:</i></b> 44-year-old female with SLE on immunosuppressants presented with dysphagia and gastrointestinal bleeding. Treatment with an anti-TB regimen and endoscopic hemostasis controlled the bleeding. Case 2: Initially presented with symptoms of gastric outlet obstruction and vomiting. Case 3: A 20-year-old female presented with severe abdominal pain. Case 4: A 24-year-old female presented with right upper quadrant pain and a palpable mass. Case 5: 60-year-old male with a history of pulmonary TB presented with severe abdominal pain and partial bowel obstruction.</p><p><b><i>Discussion:</i></b> This study describes the epidemiology, risk factors, and current diagnostic and treatment strategies of GI TB in the given cases. This case series underscores the diverse clinical manifestations of gastrointestinal TB and highlights the need for heightened awareness and timely intervention to improve patient outcomes.</p><p><b>PP-03-025</b></p><p><b>Non-cirrhotic portal hypertension from a portal vein fusiform aneurysm with arteriovenous malformation: A Case Report</b></p><p><b>John Derek Clutario</b></p><p><i>Makati Medical Center, Pasig, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Disorders associated with noncirrhotic portal hypertension are categorized based in the site of involevement. It can be prehepatic, intrahepatic or posthepatic. Our case involves both a prehepatic and intrahepatic cause of noncirrhotic portal hypertension.</p><p><b><i>Case description:</i></b> A 16 year-old male presented with severe right lower quadrant and hematochezia managed as a case of Ruptured diverticulosis and underwent Diagnostic Laparoscopy, Peritoneal Lavage, Drainage of Pelvic Abscess. He was readmitted for left lower quadrant pain now associated with loose watery stools. This event was assessed as another episode of ruptured diverticulitis. Five weeks later, he was readmitted for loss of consciousness and anemia. Endoscopy revealed esophageal varices. Rubber band ligation was done which resolved the anemia and was discharged stable. Three months after discharge, he had another episode of loss of consciousness and recurrence of anemia. Imaging revealed portal hypertension associated with a large aneurysm involving the confluence of the common hepatic artery with fistula formation to the right portal vein. He underwent another endoscopy still revealing esophageal varices where rubber band ligation was again done. The patient was stable and advised to undergo whole exome sequencing to assess for a possible connective tissue disorder particularly vascular Ehler-Danlos syndrome.</p><p><b><i>Discussion:</i></b> We have a patient presenting with abdominal pain, loss of consciousness and anemia. The search for the cause of anemia revealed esophageal varices leading to further imaging showing portal hypertension from a common hepatic artery aneurysm and arteriovenous malformation.</p><p><b>PP-03-026</b></p><p><b>Overt gastrointestinal bleeding from a pseudoaneurysm complicating a case of necrotizing pancreatitis: A case report</b></p><p><b>John Derek Clutario</b></p><p><i>Makati Medical Center, Pasig, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Acute pancreatitis is a common disease with multiple known risk factors and triggers. Mild cases have good prognosis however when severe, could lead to several complications. This case report discusses severe pancreatitis with several complications including the fistula formation and pseudoaneurysm.</p><p><b><i>Case description & discussion:</i></b> The patient is a 59 year-old male initially managaed as a case of gallstone pancreatitis and underwent subsequent cholecystectomy. Two days after discharge, he was readmitted for recurrence of epigastric pain now managed as acute pancreatitis with peripancreatic fluid collection with some necrotic component. He was medically managed and was discharged stable. Five days after, he had isolated back pain, chills and vomiting episodes. CT revealed acute pancreatitis on top of necrotizing pancreatitis with hemorrhagic component which was initially managed with antibiotics. Repeat scans revealed progression of acute hemorrhagic pancreatitis to necrotizing pancreatitis. Due to the multiloculated nature of the fluid collection, drainage was held off and medical management was maximized where the patient recovered. Two weeks after he was readmitted for hematochezia. An endoscopy was done which revealed an ulcerating friable mass with an oozing duodenal bleed where argon plasma coagulation was done. Post procedure the patient continued to be unstable hence a mesenteric angiogram was done which revealed a pseudoaneurysm and subsequent embolization was done. Clinical status was stable and repeat endoscopy with endoscopic ultrasound was done revealing a fistulous opening oozing with purulent discharge. Medical management was maximized and a nasoduodenal tube was inserted to bypass the lesions and D2 segments.</p><p><b>PP-03-027</b></p><p><b>TWIST OF FATE: Small Bowel Intussusception secondary to Peutz-Jegher Syndrome</b></p><p><b>Rial Juben De Leon</b>, Christine Marianne Sy, Ma Teresita Gamutan and Esparanza Grace Santi</p><p><i>De La Salle University Medical Center, Dasmarinas, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Acute intestinal intussusception in adults is rare, accounting for only 5% of all cases of intussusception and contributing to 1-5% of intestinal obstructions.</p><p>This study reports a case of a 33-year-old Filipino male without comorbidities who presented with sudden, severe abdominal pain, post-prandial vomiting, hematochezia, and obstipation. His family history was notable for hamartomatous polyps in his father and sibling. PE revealed hyperpigmented macules on lips and feet, hypoactive bowel sounds, and rebound tenderness in the upper abdomen.</p><p>Plain abdominal CT scan revealed small bowel intussusception. Laboratory findings were unremarkable except for mild anemia and leukocytosis. Emergency laparotomy confirmed the jejuno-jejunal intussusception and revealed palpable lesions in the colon, prompting referral to gastroenterology. Colonoscopy uncovered multiple varied sized polyps with grossly dysplastic features in the ascending and sigmoid colon. Histopathology of excised intestinal segments and colonic polyps revealed hamartomatous polyps establishing the diagnosis of Peutz-Jegher Syndrome (PJS). Post-operatively, the patient showed improvement and was discharged after a 10-day hospital stay.</p><p>PJS is characterized by progressive polyp growth, leading to complications such as pain from polyp infarction, chronic bleeding, and intestinal obstruction. It is also associated with a significantly increased risk of both gastrointestinal and extra-intestinal cancers. Treatment primarily involves surveillance and symptom management, with surgical intervention necessary for complications like intussusception. Regular surveillance every 2-3 years is recommended post-diagnosis to mitigate risks associated with PJS, including bleeding, obstruction, and cancer development. While no cure exists for PJS, early detection and intervention can significantly improve patient outcomes and quality of life.</p><p><b>PP-03-028</b></p><p><b>Unveiling the enigma: banti‘s syndrome and the mysteries of congestive splenomegaly</b></p><p><b>Rial Juben De Leon</b> and Rona Marie Lawenko</p><p><i>De La Salle University Medical Center, Dasmarinas, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>An 18-year-old, non-alcoholic, Filipino female was hospitalized following an acute episode of hematemesis and melena. No history of prior NSAID, steroids, or herbal use. With no significant prior medical conditions, the patient had pallor with signs of hypovolemia. There was no jaundice, skin lesions, abdominal pain, edema, altered sensorium, or fever. Abdominal PE was unremarkable. Severe anemia (54 g/L) and hypoalbuminemia (15 g/L) were noted. Transaminases and other liver function tests were normal. APRI score was 0.5. PBS was negative for atypical cells or malarial parasites. Viral hepatitis panel and ANA were negative. Ultrasound revealed normal-sized liver, mild splenomegaly with no signs of cirrhosis, mass, or ascites. The patient was initially started on Omeprazole and Octreotide drip and IV Ceftriaxone. Blood transfusion was done. Subsequent esophagogastroduodenoscopy revealed grade 3 esophageal varices with mild portal hypertensive gastropathy. No gastric/duodenal varices seen. Rubber band ligation was done and later on, she was started on Propanolol for secondary prophylaxis. She was eventually discharged improved.</p><p>The diagnosis of Banti's syndrome was established which is characterized by persistent splenic enlargement leading to blood cell destruction with subsequent anemia, and signs of portal hypertension without any liver pathology. While the exact pathophysiology remains unclear, the syndrome typically presents with gastrointestinal hemorrhage and splenomegaly resulting to pancytopenia. In emergency situations, endoscopic sclerotherapy and/or variceal band ligation can be performed, and beta-blockers are recommended for primary prophylaxis in portal hypertension, irrespective of cirrhosis, while splenectomy may be necessary for recurrent bleeding and severe anemia requiring multiple transfusions.</p><p><b>PP-03-029</b></p><p><b>Empyema Thoracis from Transdiaphragmatic Extension of Pyogenic Hepatic Abscess, A Case Report</b></p><p><b>Christopher Delima</b>, Lady Angela Robles and Melissa Estur</p><p><i>Divine Word Hospital, Tacloban City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Empyema thoracis as a complication of pyogenic hepatic abscess is a rare condition yet lethal. It is unusual with an incidence rate ranging from 0.5% to 0.8%.and recent literatures are limited to only 23 cases.</p><p><b><i>Case Report:</i></b> A 53-year-old female experienced right upper quadrant abdominal pain and fever two weeks prior to admission. It was associated with dyspnea. Patient is newly diagnosed with Type 2 Diabetes Mellitus. Whole abdominal computed tomography scan with contrast revealed irregular thick walled multiloculated lesion in the right hepatic lobe considering hepatic abscess with signs of capsular and diaphragmatic rupture extending to right pleural space. Patient undergone emergency exploratory laparotomy, evacuation of hepatic abscess, diaphragm repair, and closed tube thoracostomy, right. Blood and abscess cultures noted no growth. Patient observed to be clinically improving. Antibiotic therapy completed. Closed tube thoracostomy removed and discharged.</p><p><b><i>Discussion:</i></b> Pyogenic hepatic abscess is both a diagnostic and therapeutic challenge as clinical signs are nonspecific despite modern imaging techniques. Type 2 diabetes mellitus remains a strong risk factor for its development. Drainage of both the hepatic abscess and empyema with antibiotic therapy is recommended to achieve good prognosis as drainage of only one site will not be adequate for resolution of the disease Multidisciplinary management and early detection are essential to achieve good outcomes as evidence is still scarce. This case requires standard clinical guidelines and further investigation.</p><p><b>PP-03-030</b></p><p><b>Resolving Buried Bumper Syndrome with Balloon-Assisted Percutaneous Endoscopic Gastrostomy Placement: A Case Report</b></p><p><b>Ma. Regina Dimaculangan</b>, Laurence Laurel, IV Federico Peralta and Alexandra Laya</p><p><i>St. Luke's Medical Center - Global City, Taguig, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Buried bumper syndrome is a serious complication associated with long-term PEG tube placement, and management often involves endoscopic interventions. These procedures can be challenging in certain patients, hence the Balloon-assisted PEG placement (BAG) emerged as a promising option. This technique involves the use of an inflatable balloon catheter to help dilate the gastrostomy site, facilitating easier passage of the new PEG tube, and potentially reducing complications.</p><p><b><i>Case Description:</i></b> A 91 year old male with known chronic cerebrovascular disease was admitted as a case of Buried Bumper Syndrome. The PEG tube - Mushroom type, was fixed, anchored, and cannot be pushed into the stomach lumen, confirming the CT Scan findings of the PEG tube located within the anterior abdominal wall. Since the patient was unstable to undergo EGD guided PEG replacement, the team decided to try a bedside approach. A guidewire was first placed coursing through the existing PEG site. A catheter was advanced over the wire, and water soluble contrast was flushed. Spot x-ray image revealed contrast in the small bowel. The catheter was then removed, and a CRE Balloon Dilator was advanced over the wire, and inflated sequentially from 8-10mmHg to achieve the desired tract dilation. A F24 Balloon PEG Tube was then advanced and secured in place. Contrast was again flushed, and radiographs confirm its intra-gastric placement. Patient tolerated the procedure well with no untoward events.</p><p><b>PP-03-031</b></p><p><b>Ileo-colic Intussusception secondary to Primary Diffuse Large B-cell Lymphoma of the Cecum: A Case Report</b></p><p><b>Ma. Althea Kathrine Elinzano</b></p><p><i>East Avenue Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Ileo-colic intussusception refers to the invagination of the ileum into an adjoining segment of the ascending colon. Intussusception in adults is rare and is often associated with malignancy. Primary colorectal lymphoma is a rare phenomenon among malignancies affecting the colon.</p><p><b><i>Case report:</i></b> We present a case of a 67-year-old Filipino male with one month history of vague abdominal pain and unintentional weight loss. The medical history was unremarkable. He was advised colonoscopy wherein a bulky, partially obstructing, nodular mass at the cecum was noted. Histopathology and immunohistochemical staining revealed lymphoma of the diffuse large B-cell type. Contrast CT scan of the abdomen showed a segment of the terminal ileum pulled into the ascending colon. Right hemicolectomy was done. There was a 6.5x6.0x4.0 cm tan brown, doughy mass at the cecal segment, completely obstructing the lumen. Surgical margins were negative for tumor invasion, however, there was lymph node involvement. The patient was advised for chemotherapy.</p><p><b><i>Discussion:</i></b> Primary cecal lymphoma presenting with intussusception is extremely rare and requires a multi-disciplinary approach. Surgical intervention is recommended without attempting prior reduction, given the likelihood of encountering malignancy and the associated risks of perforation and tumor spread.</p><p><b>PP-03-032</b></p><p><b>Autoimmune Hepatitis and Systemic Lupus Erythematosus Overlap Syndrome: A Case Report</b></p><p><b>Ma. Althea Kathrine Elinzano</b></p><p><i>East Avenue Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Autoimmune hepatitis (AIH) and systemic lupus erythematosus (SLE) are distinct autoimmune disorders characterized by aberrant immune responses targeting various organs and tissues. However, overlap between these conditions, poses diagnostic and management challenges due to its rarity and heterogeneous clinical presentation.</p><p><b><i>Case report:</i></b> We present a case of a 40-year-old female with a 5-year history of joint pains, malar rash, undocumented fever, and jaundice. Initial laboratory tests showed elevated liver enzymes. ANA was positive, with a titer of 640, and serum IgG was elevated. She underwent liver biopsy and was compatible with autoimmune hepatitis. She was maintained on prednisolone, however, due to resolution of symptoms, the patient was lost to follow-up and discontinued her medications for two years. Subsequently, she developed recurrence of fever, jaundice, joint pains, with associated increasing trends of liver enzymes. She was diagnosed to have a relapse of autoimmune hepatitis type I, with systemic lupus erythematosus. Contrast CT scan of the abdomen and Magnetic Resonance Cholangiopancreatography (MRCP) revealed an unremarkable liver and biliary tree. Serum ammonia, liver enzymes, bilirubins, and INR were elevated. The patient underwent Double Plasma Molecular Adsorption System (DPMAS) for impending liver failure. Methylprednisolone pulse therapy was initiated and was subsequently tapered to prednisone. Clinical and biochemical improvement was noted prior to discharge from the intensive care unit. Normalization of liver enzymes and resolution of jaundice was noted on follow-up.</p><p><b><i>Discussion:</i></b> This case underscores the importance of multidisciplinary collaboration and individualized care in managing this complex autoimmune syndrome.</p><p><b>PP-03-033</b></p><p><b>Sinistral Portal Hypertension Secondary to Chronic Pancreatitis in a 20-year old Male: A Case Report</b></p><p><b>Ma Althea Kathrine Elinzano</b></p><p><i>East Avenue Medical Center, Quezon City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Sinistral portal hypertension is a rare condition characterized by the obstruction or thrombosis of the splenic vein, leading to increased pressure in the splenic circulation. Chronic pancreatitis (CP) is a progressive inflammatory disease of the pancreas characterized by irreversible damage to pancreatic tissue, leading to persistent abdominal pain, malabsorption, and impaired quality of life.</p><p><b><i>Case report:</i></b> We present a case of a 20-year-old male with a 4-year history of recurrent epigastric pain radiating to the back. He had prior multiple admissions for recurrent necrotizing pancreatitis. Contrast CT scan of the abdomen showed severe necrotizing pancreatitis, splenomegaly, and multiple dilated perisplenic and gastric collateral vessels. Splenic doppler ultrasound revealed splenic vein thrombosis. An isolated gastric varix at the cardia was seen in esophagogastroduodenoscopy. Endoscopic ultrasound showed a diffusely hypoechoic pancreatic body, with stranding, hyperechoic foci and lobulations at the pancreatic head, consistent with chronic pancreatitis. The patient was maintained on propranolol and apixaban. Hematologic work-up for JAK2 mutation and myeloproliferative disorders were unremarkable. ANA and IgG4 were within normal. The patient was advised further genetic testing for a hereditary etiology of chronic pancreatitis.</p><p><b><i>Discussion:</i></b> Although rare, understanding the less common complications of chronic pancreatitis is crucial for clinicians to effectively diagnose and manage patients with this condition, ultimately improving patient outcomes and quality of life.</p><p><b>PP-03-034</b></p><p><b>Adhesion-induced gastric stenosis four years after adjustable gastric band removal surgery</b></p><p><b>Sang Soo Eom</b> and Seok In Kang</p><p><i>Inje University College Of Medicine, Ilsan Paik Hospital, Gyunggi, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Laparoscopic adjustable gastric band surgery (LAGB) was previously performed as a treatment for obesity, but recently, due to various complications, it is gradually being ruled out. Although various complications have been reported after LAGB such as band erosion, reports of gastric stenosis after gastric band removal surgery are extremely rare. Herein, we report a rare case of adhesion-induced gastric stenosis 4 years after adjustable gastric band removal.</p><p><b><i>Case Description:</i></b> A 60-year-old female presented to the emergency department with recurrent vomiting. She had undergone LAGB 6 years prior, and the adjustable gastric band was removed 4 years ago. The laboratory results showed elevated serum creatinine (2.92 mg/dL), indicating acute kidney injury, and hypokalemia (2.6 mmol/L). Gastric stenosis was diagnosed by pre-operative computed tomography (CT) and esophagogastroduodenoscopy (EGD) (Figure 1). Laparoscopic surgery was performed to resolve stenosis, severe fibrosis encircling mid body of the stomach was in the surgical field; lymph node station 4sb and 7 were adhered to the anterior wall and posterior wall of the stomach, respectively, due to adhesion (Figure 2). Subsequently, adhesiolysis was performed, resolving gastric deformity. Post-operative CT and EGD confirmed the restoration of the stomach’s normal anatomical structure (Figure 3).</p><p><b><i>Discussion:</i></b> Despite its rarity, adhesion-induced gastric stenosis should be considered as a differential diagnosis even after removal of adjustable gastric band, necessitating adhesiolysis.</p><p><b>PP-03-035</b></p><p><b>Budesonide for Immune Checkpoint Inhibitor-associated Gastritis: A Case Series</b></p><p>Scott Mackay<sup>1</sup>, Stephen Wolman<sup>2</sup> and <b>Flavio Habal</b><sup>3</sup></p><p><sup>1</sup><i>Toronto General Hospital UHN, Toronto, Canada;</i> <sup>2</sup><i>University of Alberta, Edmonton, Canada;</i> <sup>3</sup><i>Toronto General Hospital UHN, Toronto, Canada</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Immune checkpoint inhibitor (ICI) therapies are used to treat a variety of malignancies and have well-established adverse effects throughout the gastrointestinal tract.1 “ICI-associated gastritis” is a less recognized complication of ICI therapy which can lead to significant complications of inflammation and bleeding and intolerance to oral intake.2 Literature to date reports attempts at treatment of “ICI-gastritis” with proton pump inhibitors, gastrointestinal protectants, and systemic glucocorticoids (i.e., prednisone, methylprednisolone), which are often not successful. There remains no specific guidelines for management.3 This case series summarizes four patient cases in which budesonide (Entocort®) led to dramatic symptomatic clinical resolution.</p><p><b><i>Case Series:</i></b> Four patients receiving ICI therapy developed ICI-associated dyspeptic symptoms. Their symptoms included nausea, vomiting, anorexia, weight loss, and epigastric discomfort with onset ranging from one cycle to four years after initiation of ICI therapy. Endoscopic findings were varied with having normal appearing mucosa or erythema and erosions. Pathology consistently showed increased intraepithelial lymphocytes and varying severities of inflammation. All patients reported symptomatic improvement within two to four days of starting budesonide 9 mg oral daily. Endoscopic appearance was unchanged.</p><p><b>PP-03-036</b></p><p><b>Unique chlorophyllum molybdites poisoning : role of polymeric protein in molybdophyllysin</b></p><p><b>Angkasa Hamdan</b> and Dolvy Girawan and Muhammad Begawan Bestari and Nenny Agustanti and Palar Wijaya</p><p><i>Universitas Padjadjaran, Kota Bandung, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Background:</i></b> Since time immemorial, identifying edible mushroom for consumption becomes the main problem of mushroom poisoning, even for experienced person. Mushroom toxin consist of protoplasmic, neurotoxin, and gastrointestinal irritation. However, Chlorophyllum molibdites sp. poisoning can manifest in peculiar fashion due to its unique properties of protein.</p><p><b><i>Case Report:</i></b> There were three cases presented, who suffered from various clinical manifestations after mushroom consumption during camping at the same time. A 36 years old male experienced altered consciousness and elevated level of transaminase (SGOT/SGPT: 1804/4139). Unusual ascites was found. There was no decline in renal function despite indication of acute liver failure. A 20 years old male experienced elevated level of transaminase (SGOT/SGPT: 8233/7521), jaundice, hypoalbumin and unusual ascites without altered consciousness. Lastly, a 26 year old male experienced severe diarrhea with moderate elevation of transaminase (SGOT/SGPT: 2108/2632).</p><p><b><i>Discussion:</i></b> Chlorophyllum molybdites causes most of mushroom poisoning in Indonesia. Polymeric protein of Chlorophyllum sp consist of complex arrangements which can be influenced by even demographic area and climate its found. We identified a patient with severe liver failure who didn’t manifested any decline in his kidney function, in contrast to most severe mushroom poisoning.</p><p><b><i>Keywords:</i></b> mushroom poisoning, chlorophyllum, liver failure</p><p><b>PP-03-037</b></p><p><b>Impact of pancreatolithiasis treated by using a novel slim peroral pancreatoscope</b></p><p><b>Mari Hayashi</b>, Hiroyuki Kojima, Takayoshi Tsuchiya, Reina Tanaka, Ryosuke Tonozuka, Syuntaro Mukai, Kenjiro Yamamoto, Kazumasa Nagai, Yukitoshi Matsunami, Hirohito Minami, Noriyuki Hirakawa, Kento Shionoya and Takao Itoi</p><p><i>Department of gastroenterology and hepatology, Tokyo medical university, Shinjyuku-ku, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> In symptomatic pancreatolithiasis, treatment options include Extracorporeal Shock Wave Lithotripsy (ESWL) and Endoscopic Retrograde Cholangiopancreatography (ERCP). The effectiveness of pancreatic stone fragmentation through laser lithotripsy (LL) under peroral pancreatoscope (POPS) has been reported. We present a case where pancreatic stone removal was performed using a novel slim POPS following LL.</p><p><b><i>Case Description:</i></b> An 80-year-old male with chronic pancreatitis experienced recurrent acute pancreatitis due to a 10mm pancreatic stone detected via endoscopic ultrasound in April 20XX. In May, ERCP revealed stones in the pancreas head. A drill dilator was used to penetrate the stones and advance into the pancreatic duct distal to the stones, where the POPS was then inserted. The stones were fragmented using a Yttrium aluminum garnet (YAG) laser. Due to removal difficulties, a pancreatic stent was placed. Subsequent ESWL and YAG laser treatments fragmented residual stones. However, removing these smaller fragments under fluoroscopy was challenging; thus, under POPS guidance, a retrieval basket was deployed distal to the stones, allowing for the extraction of these smaller fragments into the intestinal tract along with the POPS.</p><p><b><i>Discussion:</i></b> The use of POPS facilitates reliable stone removal and prevents basket impaction. The novel slim design allows treatment in cases with narrow pancreatic ducts, ensuring safe and definitive stone removal.</p><p><b>PP-03-038</b></p><p><b>A case of local recurrence of the cholangiocarcinoma 10 years after surgery</b></p><p><b>Kento Hisamatsu</b>, Kotaro Takeshita, Yuma Fujita and Satoshi Asai</p><p><i>Tane General Hospital, Osaka, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> In the gastrointestinal malignancies, postoperative follow-up is commonly completed after 5 years without recurrence. we experienced a case of distal cholangiocarcinoma that recurred 10 years after surgery.</p><p><b><i>Case Description:</i></b> The patient is a 67-year-old Japanese male. He underwent pancreatoduodenectomy and Billroth-II reconstruction for distal cholangiocarcinoma. The chemoradiotherapy, the follow-up was completed without recurrence at 5 years after surgery. 5 years later, he came to our hospital with a fever. CT scan showed a liver abscess and antibiotics was started. However, the contrast-enhanced CT scan showed an enlarged abscess, so percutaneous transhepatic abscess drainage (PTAD) was performed on the 14th day. Although the abscess was almost improved, contrast through the PTAD showed the abscess and posterior branch of the bile ducts were in communication and the bile drainage of the PTAD did not decrease, ERCP was performed on the 18th day. There was a stenosis at the bile duct jejunal anastomosis, which was suspected to be a recurrence. Cholangiography showed bile duct stenosis at various points in the intrahepatic ducts. Since stent placement in the posterior bile duct was a failure due to severe stenosis, stenting was endoscopically completed assisted by a PTAD route the other day. A biopsy of the stenosis at the anastomosis revealed adenocarcinoma pathologically similar to the cholangiocarcinoma of 10 years ago.</p><p><b><i>Discussion:</i></b> We report here a rare case of local recurrence of distal cholangiocarcinoma 10 years after surgery.</p><p><b>PP-03-039</b></p><p><b>Linitis Plastica in a patient with breast cancer: primary gastric cancer or breast cancer metastases?</b></p><p><b>Jonas Ho</b></p><p><i>Singapore General Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Linitis plastica is a challenging diagnosis in the setting of a patient with previous breast cancer.</p><p><b><i>Case Description:</i></b> A 74-year-old Chinese Female with a history of left breast invasive ductal cancer (ER+, PR+, HER+) 4 years prior, and completed neoadjuvant surgery and chemotherapy, with clinical remission on letrozole.</p><p>She had presented with epigastric pain, nausea, early satiety and weight loss of 5kg and an Oesophageogastroduodenoscopy (OGD) which revealed that gastric folds were thickened and prominent with overlying pangastritis associated with mucosal oedema and sloughing.</p><p>Gastric biopsies done demonstrated gastric mucosa with features of poorly differentiated adenocarcinoma with signet ring cells. Immunohistochemistry demonstrated CDX2 positivity in tumour cells. CK7 and CD20 show variable positivity in tumor cells. Staining for GATA3 and ER were performed which was negative. TTF-1 was also negative. The degree of HER2 overexpression was equivocal. The overall findings are conclusive for a primary gastric adenocarcinoma.</p><p>A computer tomography of the thorax and abdomen was performed which showed diffuse mural thickening around the distal oesophagus and stomach with enlarged perigastric lymph nodes. She subsequently underwent a diagnostic laparoscopy revealing peritoneal metastases, and was started on systemic chemotherapy.</p><p><b><i>Discussion:</i></b> Differentiating primary gastric adenocarcinoma and metastatic breast cancer is of pivotal importance given the downstream implications for treatment and prognosis. Immunohistochemistry remains the definitive means of ascertaining this where positivity for GATA3 and ER and CK7 is consistent metastatic breast cancer.</p><p>Therefore, deep biopsies or even endoscopic ultrasound guided needle biopsies are warranted when the index of suspicion is high.</p><p><b>PP-03-040</b></p><p><b>Amebic Colitis presenting with Chronic Iron-Deficiency Anaemia</b></p><p><b>Naung Latt Htun</b> and Dilip Thottacherry</p><p><i>Suri Seri Begawan Hospital, Ministry Of Health, Kuala Belait, Brunei Darussalam</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Entamoeba histolytica is an intestinal protozoan that causes a wide range of intestinal problems. It is common in many parts of the world and is transmitted by faecal-oral route. It can cause chronic iron-deficiency anaemia due to bleeding from an amoebic ulcer or colitis.</p><p><b><i>Case Description:</i></b> A thirty-year-old man presented with dizziness and fatigue. The examination was unremarkable, apart from mild conjunctival pallor. The blood tests showed haemoglobin of 10.7 with MCV 87 and transferrin saturation of 5. He underwent OGD and colonoscopy, which revealed a small duodenal ulcer and numerous 5—to 10-mm shallow ulcers in the ascending colon and caecum. A colon biopsy showed focal areas of erosions associated with mild to moderate acute inflammatory cell infiltrate. PAS histochemical stain highlights organisms with features compatible with Entamoeba histolytica. He was treated with Metronidazole for ten days.</p><p><b><i>Discussion:</i></b> As amebiasis comes with non-specific GI symptoms like diarrhoea and abdominal pain, there can be a delay in getting a diagnosis. Chronic intestinal amebiasis can lead to iron deficiency anaemia, intestinal perforation, or liver abscess. Therefore, it is essential to consider amoebic colitis as the cause of chronic iron-deficiency anaemia.</p><p><b>PP-03-041</b></p><p><b>Cured intermediate stage hepatocellular carcinoma (HCC) after single session of transarterial chemoembolization</b></p><p><b>Boy Hutaperi</b><sup>1</sup> and Putut Bayupurnama<sup>2</sup></p><p><sup>1</sup><i>Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia;</i> <sup>2</sup><i>Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Intermediate stage HCC comprises wide variety of diease phenotype. TACE is the standard of care, instead of refinement in technique and patient selection, it remains non-curative.</p><p><b><i>Case report:</i></b> A sixty-two years old female suffered dull pain around right abdomen for one month. She was controlled diabetic and negative for neither hepatitis B nor C. Physical examination found pale skin, tender hepatomegaly. Icteric, ascites and other sign of chronic liver were negative. Complete blood count showed hemoglobin 8.6 g/dL, negative finding of HBsAg, Anti-HBc and Anti-HCV, normal transaminase while liver and renal function preserved. AFP level was 1.79 ng/mL. MSCT showed right lobe liver mass, involving segmen 5,6,7 aproximately 9,0 x 8.0 x 10.0 cm fulfilled LIRADS 5 criteria, without portal vein thrombus or metastasis. Liver biopsy revealed hepatocellular carcinoma.</p><p>Selective TACE was opted as treatment modality using doxorubicin and cisplatin. Two month after TACE, MSCT showed necrotic area developed at mass location in segmen 6, next TACE was holded. Subsequent four month MSCT revealed the mass was disappeared. Patient stated as cancer free.</p><p><b>PP-03-042</b></p><p><b>Ruptured Abdominal Aortic Aneurysm Masquerading As Upper Gastrointestinal Bleeding: A Case Report</b></p><p><b>Edward Louie Ignacio</b>, Tristan Colasito and Herlyn Abasolo-Dumaguin</p><p><i>Cebu Velez General Hospital, Cebu City (Capital), Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Abdominal aortic aneurysm (AAA) is the weakening and dilatation of the abdominal aorta and is usually asymptomatic. When ruptured, it usually presents with severe abdominal pain and hypotension requiring emergency repair. However, gastrointestinal bleeding is an uncommon presentation for ruptured AAA unless with confounding complication of a rare aortoenteric fistula.</p><p><b><i>Case Description:</i></b> Presenting a case of 71-year-old female who came in with multiple episodes of hematemesis associated with epigastric abdominal pain, managed initially as upper gastrointestinal bleeding wherein esophagogastroduodenoscopy was done revealing a large submucosal extrinsic mass compressing the proximal gastric wall. CT scan of whole abdomen was done revealing a ruptured AAA with large paraaortic hematoma closely adjacent to the stomach. Immediate surgical referral for repair of ruptured aneurysm was done, however due to financial constraints and poor prognosis, opted to sign advanced directives. Patient eventually expired.</p><p><b>PP-03-043</b></p><p><b>Recurrent GI Bleeding in a Probable Hereditary Hemorrhagic Telangiectasia: A Rare Case</b></p><p><b>Fauzan Illavi</b> and Muhammad Firhat Idrus</p><p><i>Departement of Internal Medicine, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia;</i> <i>Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Hereditary Hemorrhagic Telangiectasia (HHT) is a rare genetic disorder with myriad bleeding manifestations. The manifestations encompass epistaxis to GI or even intracranial bleeding.</p><p><b><i>Case Illustration:</i></b> A-33-year-old male came with recurrent melena and severe microcytic anemia. The melena was intermittent without other bleeding manifestations. He had microcephaly without arterio-venous malformation (AVM) in his brain. His sister also had multiple melena episodes and microcephaly. Laboratory panel showed a very low Hb (Hb 5 g/dL) and mild azotemia. Upper and lower GI endoscopy showed no source of bleeding. Enteroscopy showed enteritis and multiple telangiectasias throughout jejunum and proximal ileum without active bleeding. Abdominal CT angiography did not show any abnormality. No genetic examination was performed. Intravenous PPI was administered until the bleeding subsided and the patient was discharged.</p><p><b><i>Discussion:</i></b> Small bowel bleeding is a rarer GI bleeding and should be considered as one of the HHT manifestations. The diagnosis of HHT can be made using Curacao criteria: recurrent epistaxis, mucocutaneous telangiectasia, GI telangiectasia or AVM in any organ (lung, liver, brain, spine), and a first degree relative of HHT. This patient met 2 criteria, thus classified as Probable HHT. The gene mutation in HHT includes Endoglin (ENG, type I HHT) and Activin receptor-like kinase-1 (ACVRL1, type 2 HHT). Unfortunately, genetic mutation assessment could not be assessed due to inavailability of these panels. HHT is a rare genetic disorder that might cause recurrent GI bleeding. The use of Curacao Criteria is vital to diagnose this syndrome, especially when genetic mutation is not available.</p><p><b>PP-03-044</b></p><p><b>Chronic Radiation Proctitis with Giant Ulcer of The Rectum: A Delayed and Fatal Radiotherapy Complication</b></p><p><b>Fauzan Illavi</b> and Rabbinu Rangga Pribadi and Saskia Aziza Nursyirwan</p><p><i>Departement of Internal Medicine, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Bekasi, Indonesia;</i> <i>Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Chronic radiation proctitis (CRP) is a delayed radiotherapy complication in many gynecological cases. CRP may induce colon ulceration, GI bleeding, and perforation if left untreated.</p><p><b><i>Case Illustration:</i></b> A-49-year-old female came to the ER with worsening bloody, abdominal pain and preshock condition. She had endometrial cancer with history of debulking total hysterectomy, chemotherapy, and external beam radiotherapy (EBRT). Intermittent diarrhea appeared six months after she completed EBRT and progressed to hematochezia. Laboratory panels showed severe microcytic anemia (Hb 5.6 g/dL), mild thrombocytopenia, and low eGFR (20.8). Colonoscopy revealed multiple telangiectasia from sigmoid to the rectum, multiple ulceration, and giant ulcer of the rectum. Histopathology displayed ulcerative tissue with accumulation of acute and chronic inflammatory cells which tested negative for Tuberculosis. Sucralfate enema was administered and hematochezia resolved, however the patient’s condition worsened because of sepsis due to urinary tract infection.</p><p><b><i>Discussion:</i></b> Radiotherapy in gynecological cancer therapy can lead to acute or chronic proctitis. The diagnosis might be delayed as in CRP the symptoms usually occur three months after the first radiotherapy. The patient presented with late onset of proctitis radiation, manifesting as CRP with severe anemia requiring blood transfusion. The grading of CRP in this patient was considered severe because it had caused deep ulceration, bleeding requiring transfusion, and abdominal pain. Argon plasma coagulation can be performed if active bleeding is found during colonoscopy. As no clinical colon perforation was present, surgery was not warranted and close monitoring was undertaken to monitor this complication.</p><p><b><i>Keywords:</i></b> radiation, proctitis, hematochezia, endometrial cancer</p><p><b>PP-03-045</b></p><p><b>The efficacy of alternative therapies for glucocorticoid-resistant cases of IgG4-related disease: Two case studies</b></p><p><b>Ken Ito</b><sup>1</sup>, Takehisa Ogura<sup>2</sup>, Michihiro Saito<sup>1</sup>, Yuto Yamada<sup>1</sup>, Shinya Tajima<sup>1</sup>, Masataka Kurihara<sup>1</sup>, Yousuke Okamoto<sup>1</sup> and Manabu Watanabe<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Toho University Ohashi Medical Center, Tokyo, Japan;</i> <sup>2</sup><i>Division of Rheumatology, Toho University Ohashi Medical Center, Tokyo, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> IgG4-related disease (IgG4-RD) typically responds well to glucocorticoid therapy, but retroperitoneal fibrosis (RPF) may develop during the course. Here, we report two cases where alternative therapies effectively managed for IgG4-RD.</p><p><b>Case Description</b></p><p><b><i>Case One:</i></b> A 62-year-old male presented with a 67mm pancreatic head mass and enlarged lymph node. Diffusion-weighted imaging MRI suggested pancreatic cancer, but EUS-FNA revealed no malignancy. IgG4 level was 493mg/dl, diagnosing Type 1 autoimmune pancreatitis and treated with glucocorticoids. Prednisolone (PSL) initially initially improved symptoms. However, tapering to 5mg caused retroperitoneal fibrosis (RPF) and left hydronephrosis. The PSL dose was increased again, but azathioprine was added since there was no change in RPF and hydronephrosis. Currently, RPF has decreased, with stable hydronephrosis and renal atrophy.</p><p><b><i>Case 2:</i></b> An 87-year-old male with post-gastric cancer surgery had CT findings of pancreatic lesions. EUS-FNA found no malignancy, but imaging confirmed locally advanced pancreatic ductal cancer. Treatment with gemcitabine and nab-paclitaxel showed no tumor progression. During treatment, transient salivary gland swelling, RPF, and IgG4 elevation (394 mg/dl) were observed, leading to a diagnosis of IgG4-RD. We selected intravenous cyclophosphamide treatment due to diabetes mellitus and glaucoma but discontinued it because of the development of peripheral neuropathy. Treatment was then switched to a combination therapy of PSL and mycophenolate mofetil (MMF). Currently, RPF has significantly improved, and observation continues with MMF monotherapy.</p><p><b><i>Discussion:</i></b> When RPF appears during glucocorticoid tapering or observation in IgG4-RD, it is important to consider other options, such as azathioprine or MMF.</p><p><b>PP-03-046</b></p><p><b>Duodenal Ulcer Developed as a Complication of Tsutsugamushi disease, A Korean Case</b></p><p><b>Jin Ook Jang</b></p><p><i>Pusan Nation University Yangsan Hospital, Yangsan, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Scrub typhus, or Tsutsugamushi disease, is caused by the transmission of Orientia tsutsugamushi usually through chigger mite bites. Most cases present with fever, chills, and a skin rash, and respond well to antibiotics. In cases of severe tsutsugamushi infection, various organs may be affected. We report a case of duodenal bleeding following a tsutsugamushi infection.</p><p><b><i>Case Description:</i></b> In November 2023, a patient presented to the emergency department with recurrent abdominal pain and diarrhea, which had persisted for seven days. Computed tomography imaging demonstrated wall thickening of the colon, admitted to the gastroenterology department for suspected infectious diarrhea.</p><p>Following hospitalization, a skin rash and eschar were observed, leading to the suspicion of O. tsutsugamushi. This was confirmed by the detection of O. tsutsugamushi Ab (IFA) Positive (1:5120), and doxycycline was promptly initiated. The diarrhea improved as the fever disappeared.</p><p>On the 4th hospital day, he complained of heartburn and melena. A gastroscopy revealed the presence of a semicircular ulcer in the duodenal bulb. Despite the administration of a high-dose proton pump inhibitor (PPI), the melena persisted. On the following day, exposed blood vessels were identified, and hemostasis was performed. A follow-up endoscopy conducted one week later demonstrated the absence of further bleeding, and the patient was subsequently discharged.</p><p><b><i>Discussion:</i></b> It is possible for patients to develop duodenal ulcers as a result of tsutsugamushi infections. Even after antibiotic treatment has been initiated and the fever has subsided, they may continue to experience heartburn and duodenal ulcer bleeding.</p><p><b>PP-03-047</b></p><p><b>Diagnostic and management dilemmas in stump appendicitis; A retrospective series of cases</b></p><p><b>Shehan Koshila Jayaweera</b>, A Jayathilake, S Shrishankar and S Thalgaspitiya and K Senanayake</p><p><i>University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Stump appendicitis is a rare complication in post-appendectomy patients. We report four cases presented with stump appendicitis in a review of diagnosis and management in a resource-scarce setting.</p><p><b><i>Cases:</i></b> A 48-year-old male patient with a history of open appendectomy 8 years ago was admitted with classic clinical symptoms and signs of acute appendicitis with elevated inflammatory markers. An ultrasound scan of the abdomen revealed gross inflammatory changes with fluid collection in the right inguinal fossa region. He underwent completion open appendectomy and uneventful post-operative recovery.</p><p>A 70-year-old female with multiple co-morbidities, who underwent open appendectomy 10 years back admitted with similar symptoms. She had elevated inflammatory markers and the ultrasound was unremarkable. Since she had persistent symptoms despite supportive care a CT scan was done and revealed stump appendicitis with surrounding inflammation. She was treated with 5 days of IV antibiotics and complete recovery achived.</p><p>Two male patients aged 18 and 25 respectively who underwent laparoscopic and open appendectomy 8 months and 3 ½ years back, presented with the same clinical presentation had elevated inflammatory markers and ultrasonographic inflammatory changes in RIF suggestive of stump appendicitis. Both were treated with intravenous antibiotics and completely recovered.</p><p><b><i>Discussion:</i></b> The exact clinical diagnosis of stump appendicitis is supported by sonographic evidence which is even imperative due to difficulty in adequate visualization since most appendicular stumps <5mm in size. Even though Open/laparoscopic completion appendectomy is considered the treatment of choice, depending on patient-related risk-benefit factors conservative management with antibiotics can also be considered.</p><p><b>PP-03-048</b></p><p><b>Importance of multi-disciplinary approach in management of patients with Familial Adenomatous Polyposis (FAP) syndrome</b></p><p><b>Shehan Koshila Jayaweera</b>, K Senanayake, SPB Thalgaspitiya, S Shrishankar and AB Jayatillake</p><p><i>University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Familial Adenomatous polyposis (FAP) syndrome is a rare genetic disease of autosomal dominant inheritance characterized by >100 polyps in the colon with a high risk of developing colorectal carcinoma. Diagnosed patients with classic FAP syndrome need to be evaluated for extra-colonic and extra-intestinal manifestations. Multi-disciplinary team (MDT) involvement in deciding non-surgical and surgical management, arranging family screening, and close follow-up is beneficial.</p><p><b><i>Case report:</i></b> A 33-year-old previously healthy male patient presented with frequent fresh per rectal bleeding and passage of fleshy parts during defecation. He also had symptomatic moderate anemia with recent onset weight loss of >10 kilograms and a paternal side family history of deaths due to unevaluated abdominal malignancies. Numerous size polyps >100 involving the whole length of the colon were noted in colonoscopy and diagnosis of FAP was established. Multiple small benign gastric polyps during Oesophagoduodenoscopy, benign-looking pancreatic cysts in MRCP, and congenital retinal pigment hypertrophy on eye screening were detected as other manifestations. He underwent laparoscopic-assisted total proctocolectomy with ileoanal pouch anastomoses and currently has a satisfactory quality of life. MDT involvement and patient and family concerns are considered in all steps of management, screening, and follow-up plans. His sister was diagnosed having the same syndrome and referred to the oncology team for novel non-operative management options to minimize disease progression since she didn’t consent to surgery.</p><p><b><i>Discussion:</i></b> Since patients with FAP syndrome carries a high risk of numerous malignancies, multi-disciplinary involvement in decision-making is always beneficial in formulating overall management, screening, and follow-up plans.</p><p><b>PP-03-049</b></p><p><b>Adult Ileocolic intussusception: A rare presentation of a common malignancy</b></p><p><b>Shehan Koshila Jayaweera</b>, K Senanayake, AB Jayatillake, SPB Thalgaspitiya and S Shrishankar</p><p><i>University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Ileo-colic intussusception is a rare cause of intestinal obstruction in adults. In contrast to children which is mostly idiopathic, in adults, this condition is always associated with a definitive etiology. Clinical presentation can vary depending on the cause and individual symptoms.</p><p><b><i>Case report:</i></b> A 44-year-old female patient with a background history of hypothyroidism presented with recent onset blood and mucous diarrhea, vomiting with moderate abdominal distension, and fever for 3 days duration admitted to medical casualty for further management of clinically suggestive dysentery. She had moderate anemia and slightly elevated inflammatory markers. Since she was poorly improved with antibiotics and supportive therapy, an abdominal ultrasound scan with plain X-rays was done and features of small bowel obstruction were noted. Urgent contrast-enhanced CT scan abdomen revealed evidence of ileocolic intussusception and she underwent emergency exploratory laparotomy and right hemicolectomy. Histological assessment of the resected part of the bowel showed 2cm size moderately differentiated invasive mucinous carcinoma with clear resection margins and 1 out of 14 lymph nodes containing tumor deposits in the specimen. She was referred for oncological management following uneventful post-surgical recovery.</p><p><b><i>Discussion:</i></b> Clinical diagnosis of adult intussusception is relatively difficult due to non-specific symptoms upon initial presentation. Radiological diagnosis with contrast CT scans of the abdomen is helpful in definitive diagnosis. Timely surgical interventions are essential.</p><p><b>PP-03-050</b></p><p><b>Epidermoid cyst in the Ileocolic junction presented as a large abdominal mass during pregnancy</b></p><p><b>Shehan Koshila Jayaweera</b>, K Senanayake, SPB Thalgaspitiya, AB Jayatillake and S Shrishankar</p><p><i>University Surgical Unit, Teaching Hospital Anuradhapura, Colombo, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Epidermoid cysts are benign encapsulated lumps filled with keratin materials that are usually found in skin. This is a rare case of an epidermoid cyst found in ileocolic area adjacent to appendix presented during pregnancy.</p><p><b><i>Case report:</i></b> A 36 year old multiparous female in her first trimester of third pregnancy presented with gradually enlarging mobile right sided abdominal lump with on and off pain for 3 weeks duration. On examination there was a mobile, firm palpable lump measuring 8cm×6cm in the right hypochondriac and lumbar regions. It was neither attached to skin nor solid organs clinically. She underwent ultrasound assessment and suggested Magnetic Resonant (MRI) scan for further evaluation to rule out Gastro-intestinal stromal tumor (GIST). MRI scan was done and diagnosis of duodenal duplication cyst was given. Patient underwent diagnostic laparoscopy in her second trimester due to rapid enlargement of the concerned lump.</p><p>Intraoperatively, large well circumscribed mass measuring 13cm×12cm attached to ileo-colic and appendicular wall was noted and converted to open mini laparotomy. Lesion was carefully dissected from the serosa of the concerned bowel and appendix, and sent for histology. Histology revealed a benign epidermoid cyst arising from Ileocaecal wall filled with keratin material.</p><p><b><i>Discussion:</i></b> Epidermoid cyst related to ileocaecal wall and appendix is unique with only few related cases in published literature. It is important as a rare benign differential diagnosis in mobile abdominal lumps with rapid enlargement.</p><p><b>PP-03-051</b></p><p><b>Treatment of Pyoderma Gangrenosum as an Extraintestinal Manifestation of Ulcerative Colitis</b></p><p><b>Alvin Johan</b> and Saskia Aziza Nursyirwan</p><p><i>Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Pyoderma gangrenosum (PG) is an uncommon neutrophilic inflammatory skin condition. Up to 70% of reported PG cases are associated with autoinflammatory conditions such as inflammatory bowel disease (IBD) or inflammatory arthritis.1 The treatment of PG remains challenging due to the lack of generally accepted therapeutic guidelines.2,3</p><p><b><i>Case Description:</i></b> A 19-year-old woman came to the emergency department with complaints of bloody stool and diarrhea that started one week before admission. She also experienced abdominal pain, polyarthralgia, and fever. There were painful skin ulcers in these last two weeks. On physical examination, we found tachycardia and a fever of 39,2 °C. There were well-defined ulcerations with a surrounding zone of erythema on the left chest and left groin. Laboratory examination showed anemia with hemoglobin of 7.9 g/dL and leukocytosis of 13,910 cells/μL. Stool examination showed stool with mucous and blood, with high leukocytes and erythrocytes. Colonoscopy revealed proctocolitis with continuous lesion extending from rectosigmoid to transverse colon with polypoid appearance. Tests for possible bacteria or parasite infections were negative. Histopathology examination found ulcers with distorted crypts. The swab on the ulcer bed found no bacterial growth. Diagnosis of ulcerative colitis with pyoderma gangrenosum was made. Hence, she was started on a course of oral steroids.</p><p><b>PP-03-053</b></p><p><b>Clinical case of radiofrequency ablation of recurrent ampullary adenoma</b></p><p><b>Valeriia Kamalova</b>, Evgeniy Solonitsyn, Sabina Seyfedinova and Dmitriy Baranov</p><p><i>Almazov Nmrc, Saint Petersburg, Russian Federation</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Ampullary adenomas is extremelly rare but all of them should be removed if detected. The range of surgical interventions is extremely wide from pancreaticoduodenal resection to ablation techniques depending on the clinical situation.The presented case describes a long-term recurrence of an ampullary adenoma with successful resolution after RFA. 83 years old patient has a complaint of recurrent epigastric pain. He was diagnosed with ampullary adenoma in 2009 and underwent duodenotomy with excision of adenoma.After 8 years recurrence was noted and endoscopic ampulectomy was performed. In 2024 he underwent gastroscopy on which resected area was seen with scarring changes and enlarged villi. Biopsy was taken and tissue was suspected on adenoma with high grade dysplasia. EUS was performed to assess intraductal spread where dilated common bile was seen with hypoechogenic tissues in the terminal part.A decision was made to perform intraductal radiofrequency ablation. ERCP with intraductal ablation was performed and a plastic biliary stent was installed. In the postoperative period transient hyperamylasemia without clinical manifestations was noted. At the repeated ERCP cholangiocopy was performed on which scar tissues with enhanced vascular pattern was seen. A forceps biopsy was performed and a short plastic stent was placed. At pathological examination the samples consist of focal fibrosis so there were no data for adenoma recurrence.The patient is under outpatient observation now, he has no complaints. The described clinical case demonstrates the use of the latest endoscopic techniques for preoperative diagnosis and minimally invasive intervention for successful treatment of recurrent course of ampullary adenoma.</p><p><b>PP-03-054</b></p><p><b>Chronic Dysphagia due to an Esophageal Stricture: A Case Report on Lymphocytic Esophagitis</b></p><p>Xuan Yu Tan<sup>1</sup>, Sagar Sharma<sup>1</sup>, Heather Heng<sup>2</sup> and <b>Garrett Kang</b><sup>3</sup></p><p><sup>1</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore;</i> <sup>2</sup><i>Department of Pathology, Sengkang General Hospital, Singapore;</i> <sup>3</sup><i>Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Lymphocytic Esophagitis (LE) is a rare form of chronic esophagitis, commonly presenting with dysphagia. Features of LE endoscopically include esophageal rings, linear furrows, whitish exudates, and esophageal strictures. It is characterised histologically by increased peripapillary/intraepithelial lymphocytes. The pathophysiology and aetiology of the disease are poorly understood, but has been associated with inflammatory bowel disease.</p><p><b><i>Case Description:</i></b> We present a case of a 67-year-old female who previously presented to another hospital in 2018 with chronic dysphagia. Her upper endoscopy and barium meal were normal. High-resolution manometry indicated non-specific esophagogastric junction outflow obstruction, and endoscopic ultrasound showed no abnormalities at the gastro-esophageal junction.</p><p>The patient now presented again to our hospital in 2023 with worsening daily esophageal dysphagia. She underwent an esophagogastroduodenoscopy (Figure 1) which revealed a stricture in the upper oesophagus, and patchy esophagitis in the mid and lower esophagus. Biopsy was negative for malignancy, and showed increased intraepithelial lymphocytes without significant eosinophils or neutrophils. There was minimal spongiosis. GMS stain was negative for fungal organisms.</p><p>Taking into account the overall findings, our patient was treated for LE with a course of omeprazole and offered endoscopic dilatation.</p><p><b>PP-03-055</b></p><p><b>Dysphagia from Esophagitis Dissecans Superficialis in a patient with strong smoking history, improved with omeprazole</b></p><p>Rui Kyi Ng<sup>1</sup>, <b>Ethel Ng</b><sup>1</sup>, Issam Jajeh<sup>2</sup> and Garrett Kang<sup>3</sup></p><p><sup>1</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore;</i> <sup>2</sup><i>Department of Pathology and Laboratory Medicine, Sengkang General Hospital, Singapore;</i> <sup>3</sup><i>Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Esophagitis dissecans superficialis (EDS) is a rare and benign disease, usually presenting with dysphagia. It is characterized by sloughing of the esophageal mucosa. There are associations of EDS with various medications, chemical irritants, dermatological and autoimmune diseases, but it can also be idiopathic.</p><p><b><i>Case Description:</i></b> Our patient is a 53-year-old female presenting with daily esophageal dysphagia over the past 2-3 years without heartburn or regurgitation. She is a chronic smoker of at least 15 pack years and consumes a bottle of wine daily. Esophagogastroduodenoscopy (Figure 1) revealed sloughy mucosa and whitish desquamation seen in the lower & mid esophagus. Biopsy revealed basal hyperplasia, severe hyper parakeratosis and splitting. There was no dysplasia, fungal stain was negative. She was started on a course of omeprazole 20mg twice daily and had significant symptomatic improvement, now with symptoms occurring only 2-3 times a month.</p><p><b>PP-03-056</b></p><p><b>Phlegmonous gastritis in an immunosuppressed cirrhotic patient treated with antibiotics</b></p><p>Zihan Song<sup>1</sup>, Shu Ting Chong<sup>1</sup> and <b>Garrett Kang</b><sup>2</sup></p><p><sup>1</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore;</i> <sup>2</sup><i>Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Phlegmonous gastritis (PG) is a rare and potentially fatal infection of the stomach wall, with a 10-54% mortality rate. Patients often present with epigastric pain, fever, vomiting, or bleeding. Although the pathophysiology is unknown, associated risk factors with PG include alcohol and immunodeficiency. Antibiotic treatment is often successful, although surgery may be required.</p><p><b><i>Case Description:</i></b> Our patient is a 52-year-old male, with a background of recently diagnosed ANCA & PR3 positive vasculitis with IgA nephropathy, on treatment with prednisolone & cyclophosphamide. He also had a recent diagnosis of Child B NASH cirrhosis. Esophagogastroduodenoscopy (Figure 1A) was performed for variceal screening and bloating, which revealed small varices and pangastritis. On physical examination, the patient’s abdomen was distended but non-tender. Biopsies of the stomach showed subepithelial congestion with mixed acute & chronic inflammatory cell infiltrate with neutrophils seen, and destruction of gastric glands. This was suggestive of active gastritis suspicious for PG. Non-contrast Computed Tomography of the abdomen showed diffuse thickening of the mid to distal stomach wall (Figure 1B).</p><p>PG was diagnosed based on a combination of imaging, endoscopic and histological findings. In view of the patient’s immunosuppressed status, he was treated with a course of antibiotics with ciprofloxacin and metronidazole for 2 weeks. Our patient remained well clinically on follow up.</p><p><b><i>Discussion:</i></b> This case demonstrates PG in a well but immunosuppressed patient with cirrhosis and vasculitis, that resolved with antibiotics therapy. As PG is potentially fatal, early diagnosis and treatment is crucial, even in an asymptomatic and well-appearing patient.</p><p><b>PP-03-057</b></p><p><b>Nintedanib-related colitis mimicking inflammatory bowel disease improved with Beclomethasone dipropionate</b></p><p><b>Jong Kyu Kim</b>, Yong Sung Choi, Wan Jung Kim, Hyung-Joong Jung and Eun Joo Kim</p><p><i>Daehang Hospital, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Nintedanib is an intracellular inhibitor that targets multiple tyrosine kinases and has been shown to reduce the progression of idiopathic pulmonary fibrosis. However diarrhea was reported the most common adverse effect and led to discontinuation and reduction of nintedanib. Some case reports suggest that steroid may be a treatment for nintedanib-related colitis.</p><p><b><i>Case Description:</i></b> A 35-year-old male was diagnosed with idiopathic pulmonary fibrosis 4 years ago. He had been taking nintedanib (150mg twice daily) after acute exacerbation 10 months ago. 3 months later, diarrhea and abdominal pain started. His symptoms had persisted despite taking anti-diarrheal medications. Infectious diseases were excluded from blood test and stool test. Colonoscopy revealed longitudinal linear ulcerations with friability on the transverse colon mimicking Crohn’s disease. Histopathology showed hyperplastic mucosa with chronic colitis and erosion. Nintedanib continued for idiopathic pulmonary fibrosis. He was prescribed beclomethasone dipropionate (5mg once daily). After 1 month, diarrhea improved without anti-diarrheal medications. Follow up sigmoidoscopy showed mild diffuse edematous mucosal change from transverse colon to sigmoid colon.</p><p><b><i>Discussion:</i></b> Nintedanib was effective for idiopathic pulmonary fibrosis. However the adverse effect of nintedanib was mainly gastrointestinal problems, particularly diarrhea. Although the mechanism of diarrhea was unclear, metabolites of nintedanib could be directly caused damage to the intestinal epithelium. In some reports, systemic steroid was effective for nintedanib-related colitis. Beclometasone dipropionate is synthetic glucocorticosteroid which has a powerful local anti-inflammatory effect but little systemic effect. Beclometasone dipropionate may be an option for treatment of nintedanib-related colitis ineffective to anti-diarrheal medications.</p><p><b>PP-03-058</b></p><p><b>Complete CLOSURE USING MANTIS CLIPS After Endoscopic Submucosal Dissection (ESD) for duodenal adenoma</b></p><p><b>Nobuyoshi Kodama</b>, Yorinobu Sumida, Tatsuya Matsumoto, Taisuke Inada, Kousuke Maehara, Masayuki Hijioka, Yuzo Shimokawa and Hirotada Akiho</p><p><i>Kitakyushu Municipal Medical Center, Kitakyusyu-shi, Fukuoka, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Endoscopic submucosal dissection (ESD) can lead to bleeding and perforation, especially in the thin-walled duodenum. Secure closure of mucosal defects is crucial. We report a case of successful complete closure using Mantis clips after duodenal ESD.</p><p><b><i>Case Description:</i></b> A 62-year-old male underwent upper gastrointestinal endoscopy, revealing a semi-circumferential adenoma in the descending duodenum, adjacent to the papilla. ESD was deemed feasible despite the challenging location.</p><p><b><i>Procedure:</i></b> A pancreatic stent was placed to prevent pancreatitis. ESD was performed under general anesthesia, including circumferential dissection. The dissected area was closed using 11 Mantis clips and 6 Sure Clips, avoiding the papilla.</p><p><b><i>Postoperative Course:</i></b> The patient experienced pain but no serious complications. Oral intake began on day 10, and discharge occurred on day 17. Follow-up endoscopies at 2 weeks and 2 months showed mild narrowing, but a standard endoscope passed without difficulty.</p><p><b><i>Discussion:</i></b> Clipping is crucial for preventing complications after duodenal ESD. Mantis clips, designed for sealing mucosal defects, offer easier and quicker application compared to conventional clips. They can approximate larger wound areas, enabling treatment of even large, near-circumferential duodenal tumors. This case demonstrates the potential of Mantis clips in achieving complete closure after duodenal ESD, preventing complications in challenging cases.</p><p><b>PP-03-059</b></p><p><b>EUS-Choledochoduodenostomy as Salvage Therapy for Failed ERCP in Malignant Biliary Obstruction</b></p><p><b>Hendra Koncoro</b></p><p><i>Tzu Chi Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Endoscopic retrograde cholangiopancreatography (ERCP) is the treatment of choice for biliary obstruction. However, endoscopic biliary drainage may be unsuccessful in some patients. EUS- guided biliary drainage (EUS-BD) has emerged as an alternative to percutaneous transhepatic biliary drainage (PTBD) with a high technical and clinical success rate, low risk of complications and a better quality of life.</p><p><b><i>Case description:</i></b> We described a case of endoscopic ultrasound-guided choledochoduodenostomy (EUS-CDS) from the first portion of the duodenum using self-expandable metal stent (SEMS) in a patient with distal malignant biliary obstruction in who ERCP was failed due to neoplastic invasion of the distal bile duct. A 75-year-old woman with a complaint of abdominal pain and jaundice was referred to our polyclinic from oncologist. The patient was diagnosed with stage IV urinary bladder cancer metastasis to vertebra and distal bile duct. The patient was suffering from obstructive jaundice and underwent endoscopic biliary drainage. However cannulation was not achieved on this setting and EUS- BD from the first portion of the duodenum was performed (Figs 1, 2).</p><p><b><i>Discussion:</i></b> Transhepatic biliary drainage has been the most common procedure for the treatment of malignant biliary obstruction in cases which ERCP fails due to tumor infiltration of the distal CBD. During the last decade the development of EUS has implied an alternative for biliary drainage in cases of failed ERCP, demostrating advantages over trans hepatic biliary drainage.</p><p><b>PP-03-060</b></p><p><b>Hidden danger: Aortoenteric fistula as the cause of gastrointestinal bleeding</b></p><p><b>Hendra Koncoro</b>, Andrew Jackson and Sugianto Santoso</p><p><i>St Carolus Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Aortoenteric fistula (AEF) is a rare and potentially fatal condition where the aorta forms an abnormal connection with the gastrointestinal (GI) tract, often leading to severe GI bleeding. Prompt identification and treatment are crucial for improving patient outcomes.</p><p><b><i>Case Report:</i></b> A 46-year-old man presented at St. Carolus Hospital’s Emergency Unit with nausea, upper abdominal pain, and dizziness persisting for 9 hours. He reported occasional black, blood-mixed stools over the past month. On examination, he was conscious and hemodynamically stable with anemic conjunctiva and epigastric tenderness. Lab results showed anemia (Hb 8 g/dL) leukocytosis (14,970/uL) and hypokalemia (K 3.3 mmol/L). Gastroscopy and colonoscopy ruling out active bleeding. CT abdomen with contrast detected an abdominal aortic dissection extending subdiaphragmatically to the bifurcation, alongside a left abdominal aortic aneurysm with suspected thrombosis. CT angiography confirmed the dissection from the left subclavian artery to above the iliac bifurcation (DeBakey III, Stanford B). Collaboration with vascular thoracic surgeons for endovascular aortic repair (EVAR) improved his condition, leading to discharge.</p><p><b><i>Discussion:</i></b> AEF diagnosis requires a high index of suspicion, as the classic clinical triad of bleeding, abdominal pain, and palpable abdominal mass may not be present. AEF should be considered in AAA patients presenting with GI bleeding. Timely diagnosis is challenging without prior AAA identification. EVAR is recommended for patients unfit for complex open surgery. Untreated AEF is almost always fatal, highlighting the need for early diagnosis and intervention.</p><p><b>PP-03-061</b></p><p><b>Autoimmune Pancreatitis with Pancreatic Pleural Effusion Successfully Treated with Steroid Therapy: Two Case Reports.</b></p><p><b>Hoshu Kurebayashi</b><sup>1</sup>, Hiroaki Kitae<sup>1</sup>, Kyoka Takatsuka<sup>1</sup>, Koki Yamamoto<sup>1</sup>, Yusaku Yokotani<sup>1</sup>, Mika Mazaki<sup>1</sup>, Naoto Watanabe<sup>1</sup>, Takaharu Yo<sup>1</sup>, Naoaki Akamatsu<sup>1</sup>, Tsugitaka Ishida<sup>2</sup>, Nobuhiko Yonekura<sup>2</sup>, Masanobu Katayama<sup>2</sup> and Hiroaki Yasuda<sup>2</sup></p><p><sup>1</sup><i>Omihachiman Community Medical Center, Omihachiman, Japan;</i> <sup>2</sup><i>Saiseikai Shiga Hospital, Ritto, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> There are few reports of autoimmune pancreatitis (AIP) with pancreatic pleural effusion. This report presents two cases of AIP with pancreatic pleural effusion successfully treated with steroid therapy.</p><p><b>Case Description:</b></p><p><b><i>Case 1:</i></b> A male in his fifties with a history of alcoholic acute pancreatitis presented to the ER. A CT scan showed recurrent acute pancreatitis with massive pleural effusion in the left thoracic cavity. A chest drainage tube was inserted, but its efficacy was limited. Elevated amylase levels were confirmed by thoracentesis. MRCP and EUS revealed diffuse pancreatic swelling and a high serum IgG4 level, leading to the diagnosis based on the Japanese Clinical Diagnostic Criteria for AIP 2018. After prescribing prednisolone (50mg/day, initial dose), pleural effusion was rapidly resolved.</p><p><b><i>Case 2:</i></b> A male in his seventies was referred to the outpatient with an elevated serum amylase level. A CT scan revealed pancreatic swelling and right pleural effusion. Elevated serum IgG4 and antinuclear antibody levels were also observed. ERCP and MRCP findings were consistent with AIP. Thoracentesis in right thoracic cavity proved elevated amylase level. Prednisolone (30mg/day, initial dose) was prescribed as a steroid trial. Two weeks later, a CT scan demonstrated the resolution of the right pleural effusion.</p><p><b><i>Discussion:</i></b> Management of pancreatic pleural effusion may require various strategies, such as thoracentesis, endoscopic pancreatic duct drainage, administration of octreotide, and pleural surgery in challenging cases. This report suggests that diagnosis by thoracentesis to confirm elevated amylase levels and early steroid therapy can be beneficial in avoiding unnecessary interventions.</p><p><b>PP-03-062</b></p><p><b>Effective Endoscopic Submucosal Dissection of a Huge Esophageal Liposarcoma: A Case Report</b></p><p><b>Moonwon Lee</b><sup>1</sup>, Hyun Ji Lee<sup>2</sup>, Gwang Ha Kim<sup>1</sup>, Bong Eun Lee<sup>1</sup>, Sunghoon Kim<sup>1</sup>, Dong Chan Joo<sup>1</sup>, Deajin Jung<sup>1</sup> and Cheol Woong Kim<sup>1</sup></p><p><sup>1</sup><i>Pusan National University Hospital, Busan, South Korea;</i> <sup>2</sup><i>Pusan National University Yangsan Hospital, Yangsan, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>This case report presents the successful endoscopic submucosal dissection (ESD) of a well-differentiated esophageal liposarcoma in a 51-year-old male with persistent dysphagia. The cause was initially diagnosed as a 10 cm pedunculated lesion extending from the upper esophageal sphincter to the mid-esophagus. An ESD was chosen over traditional surgery because it is less invasive. The procedure involved a precise submucosal injection and excision with special techniques to manage bleeding from a central vessel. Despite the extraction challenges owing to the size of the lesion, it was successfully removed orally. A histopathological examination of the 8.3×4.2×2.3 cm specimen revealed the characteristic features of a well-differentiated liposarcoma, including MDM2 and CDK4 positivity. The follow-up revealed no recurrence, and active surveillance has been performed since. This report highlights the versatility of ESD in treating significant esophageal tumors and provides evidence for its efficacy as a minimally invasive alternative.</p><p><b>PP-03-063</b></p><p><b>A Gastric Magnetic Foreign Body Incidentally Detected Several Years after Ingestion</b></p><p><b>Moonwon Lee</b><sup>1</sup>, Hyun Ji Lee<sup>2</sup>, Gwang Ha Kim<sup>2</sup>, Bong Eun Lee<sup>1</sup>, Dong Chan Joo<sup>1</sup>, Deajin Jung<sup>1</sup> and Sunghoon Kim<sup>1</sup></p><p><sup>1</sup><i>Pusan National University Hospital, Busan, South Korea;</i> <sup>2</sup><i>Pusan National University Yangsan Hospital, Yangsan, South korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Foreign body ingestion is commonly seen in children. However, occasionally it may also be seen among adults and is often associated with intellectual disability, psychiatric disorders, and alcoholism. Ingestion of a magnetic foreign body may cause complications such as gastrointestinal tract perforation, wherein emergency endoscopic removal of the foreign body is generally required. Here, we report a rare case of a 59-year-old male with an intellectual disability and psychiatric disorder in whom metallic objects in the stomach cavity were accidentally discovered during abdominal CT. Esophagogastroduodenoscopy revealed several metallic objects attached to two magnets, which had been ingested several years before and had remained in the stomach cavity. The magnets and metallic objects were safely removed endoscopically using rat-tooth forceps without complications.</p><p><b>PP-03-064</b></p><p><b>Dissecting through the diagnostic complexities of solid-cystic pancreatic tumours</b></p><p><b>Wei-Qiang Leow</b> and Samuel Jun Wei Tho</p><p><i>Singapore General Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> We dissect through the differential diagnoses for a solid-cystic pancreatic tumour, using a case of a pancreatic acinar cell carcinoma, to highlight morphological similarities and differences. We also discuss on use of immunohistochemistry, particularly BCL-10 and its pitfalls.</p><p><b><i>Case Report:</i></b> We present a diagnostic dilemma of a forty-four year-old gentleman who presented with abdominal pain secondary to an 18cm solid-cystic pancreatic tumour. Histologically, the tumour was composed of epithelioid tumour cells with abundant granular cytoplasm and prominent nucleoli. Extensive necrosis and cystic degeneration was also noted. We discuss a list of differential diagnoses including neuroendocrine tumour, solid pseudopapillary neoplasm and acinar cell carcinoma. Initial immunohistochemistry revealed focal positivity for neuroendocrine markers and a moderately high cell proliferative index (Ki-67) of up to 35%. Although BCL-10 performed in our laboratory was negative, a second consultation at an overseas laboratory confirmed the diagnosis of pancreatic acinar cell carcinoma with a positive trypsin immunohistochemical stain.</p><p><b><i>Discussion:</i></b> We discuss the morphological similarities and differences of solid-cystic pancreatic tumours, including the classical immunohistochemical profiles. We delve deeper into the reasons for the discordant BCL-10 immunohistochemistry findings.</p><p><b>PP-03-065</b></p><p><b>Multiple Gastric Polyposis: Familial Adenomatous Polyposis</b></p><p><b>Andhiky Raymonanda Madangsai</b> and Muhammad Firhat Idrus</p><p><i>Pertamina Jaya Hospital, Jakarta, Indonesia;</i> <i>Cipto Mangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Familial adenomatous polyposis is an autosomal dominant polyposis syndrome characterized by mutations in the adenomatous polyposis coli gene1. If high-grade dysplasia is detected in any gastric polyp, endoscopic or surgical resection is recommended1. Endoscopic resection (ER) with bipolar snare, in which the electric current only passes through the tissue between the device’s two electrodes, is a prominent method used to prevent perforation due to electricity potentially. ER with bipolar snare is expected to have good treatment outcomes in 10–15 mm colorectal lesions, with high safety even without submucosal injection2.</p><p><b><i>Case Report:</i></b> A 56-year-old female came with worsening epigastric pain around 3 years before hospital admission. The pain felt continuously, radiates from the back, chest, and lower abdomen. The patient complained about heartburn since a year ago, but it has gotten worse since 2 weeks. Nausea and vomiting occur when the pain is coming, food intake is around quarter portion meals per day. On March 2022, this patient underwent an EGD and the result was found polyp gaster. Patient referred to Cipto Mangunkusumo Hospital for esophagogastroduodenoscopy for polypectomi.</p><p>Polypectomy was performed with hot snare technique on the 10 largest polyps (size varies around 5-10mm). There was no active bleeding after the polypectomy.</p><p><b><i>Conclusion:</i></b> In this case, the familial adenomatous polyposis being resected by hot snare (size approximately around 5-15 mm). By this case, hot snare resection techniques can be effectively used for polypectomy. However, more studies are necessary to evaluate any other resection methods.</p><p><b>PP-03-066</b></p><p><b>Surgery vs Non-Surgery Treatment for Diverticulosis in Elderly</b></p><p><b>Andhiky Raymonanda Madangsai</b> and Haya Harareed</p><p><i>Pertamina Jaya Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Diverticulosis is a clinical condition in which multiple sac-like protrusions (diverticula) develop along the gastrointestinal tract. The majority of individuals with diverticulosis are asymptomatic but some other have symptom like diverticular bleeding1. Surgery is required for unwell patients or those with advanced disease2. The non-operative management begins with stabilization of the patient, obtaining pertinent history, and an appropriate work-up, which often includes technetium-tagged red blood cell scans, mesenteric angiography, and colonoscopy. For colonoscopy, these include cautery, epinephrine injection, and endoclips. These modalities may be utilized as a bridge to surgery, or in select instances as a definitive therapy obviating the need for surgery3.</p><p><b><i>Case Report:</i></b> An 84-year-old female came with melena since 3 days before hospitalized. On 16th May 2024, this patient underwent an EGD and the result came with colon diverticulosis (suspected source of bleeding from diverticula, but currently no active bleeding) and third grade of internal haemorrhoids. The patient is planned to undergo a hemoclip or Argon plasma coagulafion (APC) if recurrent bleeding occurs. Laboratory result shows hemoglobin levels only 5,9 g/dL. 4 packs PRC transfusion was performed to this patient.</p><p><b><i>Conclusion:</i></b> In this case, colonoscopy ligation performed for diverticulosis in elderly. Surgery is not the main option for this patient because the elderly are at high risk to do surgery. From this case, further research in medical management for diverticulosis in elderly still needs to be studied further.</p><p><b>PP-03-067</b></p><p><b>Twice tried, Twice failed… What next?</b></p><p><b>Rishikesh Malokar</b>, Vishal Malokar, Sanjay Chandnani and Pravin Rathi</p><p><i>Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction and Aim:</i></b> POEM usually has excellent clinical success.</p><p>In a study, the long-term clinical success of POEM in years 1, 2, 5, and 7 was 98%, 96%, 92%, and 91%, respectively. Two percent are non-responders at the primary level after the procedure. We describe a case of Achalasia Cardia Type II, who, in spite of undergoing POEM twice, did not have relief of symptoms and had clinical failure.</p><p><b><i>Case Description:</i></b> 35 Y/ M Dysphagia x 1 yr UGIScopy (Outside) - Achalasia Cardia, Eckardt Score 8, HRM ( Outside), Basal LES pressure – High, IRP 43. Underwent Posterior POEM (Outside). Symptoms recurred after one month. In our hospital, ECKARDT- 6. UGI Scopy - Mild resistance at GE Junction Achalasia Cardia HRM- Raised IRP 17.5mmHg, ( Achalasia Cardia II) Underwent Anterior POEM. Symptoms recurred after one month. ECKARDT score 5 Current HRM Median IRP 14.36 mmHg, Ineffective oesophagal motility Planned for Pneumatic balloon dilatation.</p><p>If it fails, 3rd POEM…?</p><p><b><i>Discussion:</i></b> Pathophysiology in recurrence could be due to the development of fibrosis and regeneration of fibres. Third POEM is technically challenging as there’s an issue with the unnatural scope orientation of submucosal fibrosis and an increased risk of bleeding.</p><p>Here, in this case we had exhausted our options and gave an option of PD.</p><p>In a multicentric study by Ichkhanian Y et al., clinical success of Repeat POEM> PD> LHM after failed POEM</p><p><b><i>Conclusion:</i></b> Pathophysiology of recurrence and optimal management of such cases is still unclear. Data regarding3rd POEM is sparse</p><p><b>PP-03-068</b></p><p><b>Reversible cause of Gastroparesis: Disseminated Koch</b></p><p><b>Rishikesh Malokar</b>, Shubham Jain, Sanjay Chandnani, Siddhesh Rane, Harsh Gandhi and Pravin Rathi</p><p><i>Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Idiopathic gastroparesis includes broad category of aetiologies. Viral infections like EBV, CMV, Varicella, and HSV are known to cause gastroparesis. The complex mechanisms include neuroimmune response against the enteral nervous system and post-infectious dysautonomia. Tuberculosis causing gastroparesis is rare entity. We describe a case of Reversible Gastroparesis due to disseminated tuberculosis improved completely post anti-tubercular therapy</p><p><b><i>Case description:</i></b> A 24-year-old female presented with abdominal pain and persistent vomiting for 1 year in 2019.</p><p>She also had intermittent fever, cough, and weight loss of 15kg in 1 year (2019)</p><p>HRCT chest and CT abdomen showed pulmonary Koch with periportal lymph nodes with urogenital Koch. Upper GIScopy (2019) showed food residue in stomoch.</p><p>Gastric emptying was markedly prolonged for both solid and liquid on scintigraphy.</p><p>Patient had repeated hospitalization for persistent vomiting. She received NJ feed initially then oral gastroparesis diet. No improvement. MTB with HR resistance detected in sputum.</p><p>The patient was started on a bedaquiline-based regimen with dietary modifications. The patient improved slowly over two years of therapy. Repeat gastroscopy, gastric emptying study (2024), and electrogastrography (EGG) (2024) were normal.</p><p><b><i>Discussion:</i></b> In idiopathic gastroparesis, pathophysiology could be due to</p><p>Neuroimmune processes targeting ENS.</p><p>Systemic dysautonomia.</p><p>Condition is rarely reversible and requires lifelong dietary modifications and prokinetics.</p><p>Tuberculosis is a rare cause of gastroparesis. Gastroparesis needs to be considered a D/D for persistent vomiting. Antitubercular treatment can improve the neuroimmune process and motility.</p><p>Further microscopic, autoantibody and gastric motility studies required.</p><p><b><i>Conclusion:</i></b> Disseminated Koch is reversible cause of gastroparesis</p><p><b>PP-03-069</b></p><p><b>CROHN’S DISEASE MIMICKING A PENETRATING SIGMOID TUMOR</b></p><p><b>Intan Aaroni Md Isa</b> and Syuhada Dan Adnan</p><p><i>Gastroenterology and Hepatology Unit, Department of Internal Medicine, Hospital Sultanah Nur Zahirah, Kuala Terengganu, Malaysia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Crohn’s disease has been known to imitate other illnesses such as tuberculosis, diverticulitis and others including tumors. The usual site of colonic Crohn’s disease, as observed in the past, is at the ileocecal area and it is usually presented as a stricturing disease. Tumefactive Crohn’s disease is mostly infrequent, what more in the sigmoid area.</p><p><b><i>Case Description:</i></b> We present a rare case of penetrating sigmoid pseudotumor of Crohn’s disease of a 59 years old gentleman who came with a 6 months history of diarrhoea and constitutional symptoms. Initial colonoscopy found mild colitis in the rectosigmoid area and biopsy revealed an active colitis with subtle chronicity where he was treated for infection. Subsequently, he developed clinical features of intestinal obstruction which emergency laparotomy done unveiled a tumor at sigmoid with infiltration to the bladder, and diversion transverse colostomy was decided intra-operatively. Colonoscopy post-operation found a rectosigmoid mass 20 cm from the anal verge and the biopsy showed an active colitis with mild chronicity. With the differential diagnosis of malignancy in mind, a repeated colonoscopy and biopsy were done. However, the verdict remained ambiguous. Consequently, the serial biopsy samples were sent for a second opinion from an IBD-Histopathologist which concluded the features of Crohn’s disease</p><p><b><i>Discussion:</i></b> Dealing with tumefactive sigmoid lesion should warrant a higher clinicians’ suspicion of possibility of Crohn’s Disease, other than malignancy. Added to that, multiple biopsies reviewed by experienced pathologists would tremendously contribute for diagnosis.</p><p><b>PP-03-070</b></p><p><b>Endocytoscopy for optical biopsy of pediatric eosinophilic esophagitis</b></p><p><b>Anastasiya Merkulova</b></p><p><i>Clinical And Research Institute Of Emergency Pediatric Surgery And Trauma, Moscow, Russian Federation;</i> <i>Institute of continuing medical education and professional development, Pirogov Russian National Research Medical University, Moscow, Russian Federation;</i> <i>Morozov Children’s City Clinical Hospital, Moscow, Russian Federation</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Eosinophilic esophagitis (EoE) is one of the most common causes of dysphagia in adults and children. Pathomorphological diagnosis (at least 15 eosinophils) is essential and needs at least 6 biopsies from esophageal mucosa. The same - at follow-up endoscopies. Endocytoscopy claims to be the novel diagnostic technique for EoE verification in vivo.</p><p><b><i>Case report</i></b>: A 13-year-old boy with complaints of abdominal pain and moderate swallowing difficulties was admitted to CRIEPST. Esophagogastroduodenoscopy was held twice: before the treatment and 1 month after. At initial endoscopy active EoE (E1R0E2F1S0), erosive HP-gastritis and acute ulcer of duodenal bulb were detected. After chromoscopy of the esophageal mucosa with 1% methylene blue endocytoscopy with optical magnification up to x520 was performed. Multiple cells with blue bilobed or bean-shaped nuclei and non-stained periphery (eosinophils) were identified among squamous epithelial cells (Fig.1). Biopsy was taken at the site of endocytoscopic observation for histological confirmation of EoE, which revealed eosinophilic infiltration (36-54 eosinophils) of the esophageal mucosa (Fig.2). After therapy with proton pomp inhibitors follow-up endoscopy with endocytoscopy detected isolated single eosinophils in esophageal mucosa (Fig.3). Histology confirmed the effective outcome: there were 0-4 eosinophils in biopsy samples from esophageal mucosa (Fig.4).</p><p><b><i>Discussion</i></b>: This case demonstrates that esophageal eosinophilic infiltration in children can be revealed by endocytoscopy. It allows to decrease the number of biopsies, exclude false negative histological findings, estimate the effectiveness of treatment in children with EoE. This case is by far the first implementation of endocytoscopy in children in Russia. Further research is needed.</p><p><b>PP-03-071</b></p><p><b>An autopsy case of pancreatic cancer with bone marrow carcinomatosis : a case report</b></p><p><b>Yumi Murashima</b>, Hiromi Ejima, Mayuko Tomeoku, Tetsuhiro Ueno, Tsuyoshi Beppu, Yuji Inagaki, Shinji Urade, Masabumi Kaneko, Hiroaki Naota, Hiroko Sugimoto and Kazuhiko Kobayashi</p><p><i>Matsusaka Chuo General Hospital, Matsusaka, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Bone marrow carcinomatosis is very rare in pancreatic cancer, which can cause disseminated intravascular coagulation (DIC) and has poor prognosis. We report herein a case of pancreatic cancer with rapid clinical course in which bone marrow carcinomatosis was found at autopsy.</p><p><b><i>Case report:</i></b> A 75-year-old man visited emergency department of our hospital with upper abdominal pain and fatigue lasting 10 days. Vital signs were normal. Laboratory test revealed abnormal liver function, thrombocytopenia, the presence of erythroblasts and postmyelocytes, and DIC. Computed tomography (CT) showed the mass of pancreatic body, multiple hepatic masses and only a few nodular shadows at lungs. Pancreatic cancer was suspected, but pathological examination could not be performed due to thrombocytopenia. Hypoxemia of unknown cause was gradually progress, and he died on the third day of admission. Autopsy showed a 35 mm mass in the body of the pancreas, and pathology showed poorly differentiated carcinoma. Similar tumor cells were found in the liver and lungs, and the bone marrow was replaced by tumor cells. In addition, there was tumor embolization in small intrapulmonary arteries, which could cause progressive hypoxemia. We diagnosed bone marrow carcinomatosis in pancreatic body cancer, which was considered to cause DIC and lead to hypoxemia and rapid death.</p><p><b><i>Discussion:</i></b> This is the case that had a typical clinical course of bone marrow carcinomatosis, but we didn’t diagnose it before death. In case of pancreatic cancer with DIC, the possibility of bone marrow carcinomatosis should be should be considered in the differential diagnosis.</p><p><b>PP-03-072</b></p><p><b>Probiotics Effect in Liver Cirrhosis Patient : An Evidence Based Case Report</b></p><p><b>Dewi Mustikarani</b></p><p><i>Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Liver cirrhosis can progress to a neurological complication named hepatic encephalopathy. Cirrhosis kills 1.3 million individuals a year and affects over 160 million people. It is critical to discover efficient therapies to stop severe liver damage. Studies have shown that the progression of cirrhosis is associated with inflammatory changes and dysbiosis.</p><p><b><i>Case Description:</i></b> A 22-year-old male patient with a previous history of liver cirrhosis was admitted to the emergency room with an altered mental status. The patient was previously given lactulose from his last admission two weeks ago, but he stopped taking lactulose for three days on his account and has not been able to defecate ever since. The patient’s has edema peritibial and massive ascites. The patient was then diagnosed with a third-degree hepatic encephalopathy. The patient was previously advised to take probiotics but has not yet taken them.</p><p><b><i>Discussion:</i></b> The inclusion and exclusion criteria were met by two meta-analyses conducted by Yang et al. (2024) and Wibawa et al. (2023). Probiotics significantly reverse minimal hepatic encephalopathy (MHE), as demonstrated by Yang's research (RR 1.54, 95% CI: 1.03 to 2.32). Probiotics have been shown by Wibawa to considerably reduce serum ammonia as compared to a placebo (p = 0.04, 95% CI: –50.21 to –1.66).</p><p><b><i>Conclusion:</i></b> The results showed that compared to a placebo or no treatment, probiotics significantly reverse MHE and reduce serum ammonia levels. Further research is encouraged to establish probiotics as one of the routine drug choices in liver cirrhosis.</p><p><b><i>Keywords:</i></b> probiotics, cirrhosis hepatitis, gut, dysbiosis</p><p><b>PP-03-073</b></p><p><b>Cytomegalovirus (CMV) Esophagitis Presenting as Upper Gastrointestinal Bleeding: A case report</b></p><p><b>Rangga Novandra</b>, Rabbinu Rangga Pribadi and Robert Sinto</p><p><i>RSUPN Ciptomangunkusumo, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Cytomegalovirus disease of the gastrointestinal tract is a major cause of morbidity and mortality in immunocompromised patients. Esophagitis is the most common GI manifestation of CMV infection after colitis. Standard diagnosis requires biopsy and pathologic verification. Current medical treatment in this disease still consist of ganciclovir and valganciclovir.</p><p><b><i>Case Illustration:</i></b> Male patients, 58 y.o, came to emergency department with complaints of hematemesis and melena. Patient denied any complaints of odynophagia and dyspaghia. He reported abdominal pain and jaundice since 5 months. The patient has a history of hematemesis, melena, and history of repeated hospitalization for transfusion since 10 years. He had no history of taking antiplatelet, oral anticoagulan or, using long-term NSAID.</p><p>In laboratory examination shows anemia, elevated serum transaminase and bilirubin. Result of HBs Ag, Anti-HCV, and Anti-HIV are nonreactive.</p><p>From endoscopic examination show solitary single transversal ulcer in the distal esophagus with firm demarcation, biopsy sample was taken with real time PCR quantitative CMV value 79.6 x 104. Patient received ganciclovir 500 mg iv/day, devide in 2 doses.</p><p><b><i>Discussion:</i></b> Diagnosis of CMV esophagitis is based on clinical history, endoscopic features, and histopathologic features. Clinically, the most common symptoms of CMV esophagitis are odynophagia, dysphagia, and chest pain. Histopathology with specific immunohistochemical stains or deoxyribonucleic acid polymerase chain reaction using tissues are required for definitive diagnosis of CMV esophagitis.</p><p>Although histopathology with specific IHC stains is the gold standard for the diagnosis of CMV esophagitis, endoscopic features are important for empirical treatment prior to histopathologic diagnosis.</p><p><b>PP-03-074</b></p><p><b>Systemic Lupus Erythematosus in a Patient with Inflammatory Bowel disease: Co-existing or SLE in Evolution?</b></p><p><b>Bea Regine Panganiban</b>, Janika Adrienne Balane and Ma. Lourdes Daez</p><p><i>Philippine General Hospital, Ibaan, Batangas, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE) are chronic immune disorders marked by relapses and remissions. The coexistence of these conditions is rare, with an estimated 0.4% prevalence of ulcerative colitis in SLE patients, and much less with Crohn’s disease. It represents a diagnostic challenge, as they have many similar gastrointestinal manifestations.</p><p><b><i>Case Description:</i></b> A 20-year-old female was diagnosed with IBD in 2019 after presenting with persistent diarrhea, abdominal pain, and pallor. Initial workup revealed hemolytic anemia and thrombocytopenia, leading to a presumptive diagnosis of autoimmune hemolytic anemia. Despite negative autoantibody tests, high clinical suspicion of SLE prompted further evaluation. Radiologic and endoscopic findings were consistent with IBD, and biopsy showed chronic colitis without granulomas. Bacterial, parasitic, viral, tuberculous and malignant etiologies were also ruled out. Treatment with 5-ASA and steroids led to symptom resolution. However, in 2022, the patient was readmitted with edema and laboratory evidence of SLE, including positive ANA, elevated anti-dsDNA, decreased complement, and active urinary sediment. Although planned for pulse steroid therapy, the patient died from nosocomial pneumonia.</p><p><b><i>Discussion:</i></b> This case highlights the uncommon association between IBD and SLE, prompting the possibility that our patient, despite initially negative autoantibodies, may have had a developing SLE that evolved into complete lupus over time. It underscores the importance of maintaining a high index of suspicion for SLE in IBD patients with complex or atypical presentations, as timely identification can significantly impact prognosis. Studies are needed to justify surveillance for autoimmune antibodies in patients with IBD.</p><p><b>PP-03-075</b></p><p><b>Navigating Risks: A Case of Esophageal Perforation During OTSC Application for ERCP-Related Duodenal Perforation</b></p><p><b>Bea Regine Panganiban</b>, Carina Samone Gregorio and Eric Yasay</p><p><i>Philippine General Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Perforations during ERCP is a rare but dreadful complication. The advent of over-the-scope clips (OTSC) has enabled effective endoscopic management of iatrogenic perforations, achieving high success rates. However, complications can arise from its use.</p><p><b><i>Case Description:</i></b> A 94-year-old female with hypertension and chronic kidney disease presented with abdominal pain and jaundice, and was diagnosed with choledocholithiasis on imaging. Initial attempts at ERCP failed, necessitating percutaneous transhepatic biliary drainage (PTBD). A year later, due to discomfort with PTBD, conversion to internal biliary stenting was desired. ERCP was performed, however intra-procedure, cholangiography demonstrated an abnormal perinephric gas shadowing prompting cessation of ERCP. Subsequent endoscopy confirmed a 2.0x1.5 cm perforation at the lateral duodenal wall. Attempts to repair the perforation with endoscopic hemoclips were unsuccessful, hence OVESCO was deployed, achieving good tissue apposition. Shortly after, the patient developed signs of pneumothorax, requiring chest tube insertion. On follow-up endoscopy, a 15mm esophageal mucosal tear was identified and closed with e hemoclips. The patient was conservatively managed and showed no signs of leakage on gastrointestinal series by day 6. She remained stable and was discharged on day 10, tolerating a full diet.</p><p><b><i>Discussion:</i></b> With only two cases reported in literature to date, this case illustrates the rare occurrence of esophageal perforation due to OTSC deployment, emphasizing the risks associated with advanced endoscopic techniques particularly in elderly patients with comorbidities. While OVESCO clips are promising for closing gastrointestinal perforations, this case highlights the necessity for meticulous technique and recognition of potential complications.</p><p><b>PP-03-076</b></p><p><b>Rare Occurrence of Gastric and Gallbladder Metastases in Non-Small Cell Lung Carcinoma: A Case Report</b></p><p><b>Federico Iv Peralta</b>, John Erwin Dumagpi, Raiza Rodriguez and Gerardo Cornelio</p><p><i>St. Luke's Medical Center - Global City, Taguig, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Gastrointestinal metastasis from lung cancer (GMLC), particularly non-small cell lung carcinoma (NSCLC), is a rare and complex condition. NSCLC primarily targets the lungs, but it can metastasize to the gastrointestinal tract, with gastric and gallbladder involvement being particularly uncommon. The rarity of gastrointestinal metastasis complicates diagnosis as it can mimic benign conditions, leading to potential delays in treatment. Symptoms such as gastrointestinal perforation, hemorrhage, and obstruction may occur, although many patients remain asymptomatic. Diagnostic tools including endoscopy, CT scans, and PET scans are essential for accurate identification. Despite advancements in diagnostics and treatment, the prognosis for NSCLC patients with gastrointestinal metastases remains poor.</p><p><b><i>Case Presentation:</i></b> This report describes an 80-year-old female with Stage IVB NSCLC, who developed new abdominal symptoms. A routine surveillance PET scan revealed a new hypermetabolic lesion at the lesser curvature of the stomach and a distended gallbladder. Subsequent esophagogastroduodenoscopy and surgery identified a gastric ulcer and an inflamed gallbladder which were biopsied and removed. Histopathological examination revealed adenocarcinoma. Immunohistochemical staining (CK7, CK20, TTF1, and Napsin A) confirmed the diagnosis of gastric and gallbladder metastases from primary lung cancer. Following these findings, the patient’s treatment was adjusted to include Pemetrexed chemotherapy alongside continued Osimertinib.</p><p><b><i>Discussion:</i></b> This case highlights diagnosing GMLC's clinical challenge due to its rarity and non-specific symptoms. Heightened clinical awareness and comprehensive diagnostics are crucial for NSCLC patients with new gastrointestinal symptoms. Understanding clinical presentations, diagnostic challenges, and management strategies for NSCLC gastrointestinal metastases are vital for improving outcomes and guiding future research.</p><p><b>PP-03-077</b></p><p><b>Herpes simplex oesophagitis in immunocompetent individuals</b></p><p><b>Zeeshan Pervaiz</b>, Ahtsham Zafar, Asif Yasin and Ali Qamar</p><p><i>Walsall Healthcare Nhs Trust, Walsall, United Kingdom</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Herpes esophagitis commonly affects immunocompromised patients, especially those who have undergone organ transplants and are on antitumor drugs. Symptoms include chest pain, difficulty swallowing, and nausea or vomiting. Timely diagnosis is crucial for effective treatment, typically involving antiviral medications like acyclovir. Although rare in immunocompetent individuals, stress can reduce immunity and lead to herpes skin infections. Few cases of herpes esophagitis have been documented in immunocompetent patients.</p><p><b><i>Case Report:</i></b> A 51-year-old man with a history of mild erosive gastritis and psychosis presented with a 5-day history of nausea and vomiting and was found unresponsive with a low GCS score. Initial assessment revealed electrolyte imbalance, acute kidney injury, elevated lactate levels, and raised inflammatory markers. A CT scan identified a cystic mass causing partial gastric obstruction. The patient required intensive care and was started on antifungal medication and acyclovir. An esophagogastroduodenoscopy revealed multiple superficial volcano-like ulcers, and biopsies confirmed herpes simplex virus (HSV) esophagitis. He was treated with intravenous acyclovir and fluconazole.</p><p><b><i>Discussion:</i></b> HSV esophagitis typically affects patients with compromised immunity, but this patient had no clinical signs of immunosuppression. The severity of HSV infection is influenced by the site of involvement, immune status, and whether the infection is primary or recurrent. Endoscopy often reveals lesions in the distal or mid-esophagus. HSV-1 is primarily associated with herpes esophagitis, though rare cases of HSV-2 have been reported.</p><p><b><i>Conclusion:</i></b> This case underscores the rare occurrence of herpes simplex esophagitis in immunocompetent individuals. Prompt antiviral therapy can effectively alleviate symptoms.</p><p><b>PP-03-078</b></p><p><b>A Unique Case of a Triple Primary Gastrointestinal Malignancy in a Filipino Male</b></p><p><b>Matthew Joseph Po</b> and Jenny Limquiaco</p><p><i>Chong Hua Hospital, Cebu City (Capital), Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The occurrence of multiple primary malignancy (MPM) is an uncommon scenario that is characterized by the presence of two or more malignancies that are histologically distinct. Although the frequency of MPM is increasing, cases of three primary gastrointestinal malignancies are exceedingly rare. This case report presents an unprecedented case involving hepatocellular carcinoma (HCC), gastric gastrointestinal stromal tumor (GIST), and pancreatic adenocarcinoma.</p><p><b><i>Case Presentation:</i></b> A 68-year-old Filipino male was incidentally diagnosed with a hepatic mass during routine ultrasound. Contrast CT scan and percutaneous liver mass biopsy were both consistent with HCC. Surgical resection successfully removed the liver mass, but a gastric nodule was seen intraoperatively. The nodule was resected and sent for histopathology and immunohistochemical staining. Results were consistent with a gastric GIST, for which the patient received adjuvant treatment with Imatinib. Sixteen months later, a metachronous pancreatic adenocarcinoma was discovered. Radical surgery and adjuvant chemotherapy were administered, yielding a favorable postoperative course.</p><p><b><i>Discussion:</i></b> Although there have been previous reports of MPM in the gastrointestinal system, none have reported the combination of the tumors seen in this case. In terms of risk factors, there is minimal overlap between those seen in these cancers, most particularly advanced age. Likewise, apart from individual surgical resection of each of the tumors, there is no overlap in the management of these malignancies, quite possibly making it a challenge. Fortunately, the early diagnosis of this case prompted surgical resection of the tumors, along with selected adjuvant chemotherapy.</p><p><b>PP-03-079</b></p><p><b>Antibiotic treatment in acute cholangitis geriatric patient with septic shock: Case report and therapeutic insights</b></p><p><b>Taswin Prawira</b><sup>1</sup> and Dadang Makmun<sup>2</sup></p><p><sup>1</sup><i>Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Acute cholangitis is a serious infection of the bile ducts, typically caused by an obstruction, often due to gallstones. Immediate treatment and choice of antibiotics, particularly in geriatric patients, are critical as acute cholangitis can lead to severe complications, including sepsis and death.</p><p><b><i>Case Description:</i></b> An 83-year-old female with severe abdominal pain and fever was admitted to the emergency department. She had progressively increased jaundice within a week and severe abdominal pain a few hours before admission. Physical examination revealed decreased sensorium, low blood pressure, and icteric sclera. Severe tenderness of the right upper quadrant of the abdomen was also noted. Laboratory examination revealed a haemoglobin level of 10.9 g/dL, WBC count of 26,600 cells/mm³, platelet count of 50,000 cells/mm³, and D-Dimer of 5.07 mg/L. CT scan of whole abdomen revealed multiple choledocholithiasis in the common bile duct, causing obstruction and dilation of intrahepatic and extrahepatic bile ducts. Patient was diagnosed as acute cholangitis. Meropenem was initially administered as an antibiotic, followed by an ERCP procedure with sphincterotomy to remove stones and drain lots of pus. A culture of bile aspirate revealed Pseudomonas aeruginosa and Extended-spectrum beta-lactamase-positive Klebsiella pneumoniae with multi-drug resistance. A novel antibiotic, Ceftazidime-avibactam was administered in accordance with the sensitivity test, and patient's condition was improved significantly. She was discharged in good condition after seven days of administration of antibiotics.</p><p><b>PP-03-080</b></p><p><b>Eosinophilic Enteritis Disorder presented as chronic abdominal pain- a rare case report</b></p><p><b>Hendri Priyadi</b>, Muhammad Bestari, Dolvy Girawan, Nenny Agustanti and Eka Nugraha</p><p><i>Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Eosinophilic enteritis (EE) is a rare gastrointestinal disorder characterized by eosinophilic infiltration of the intestinal wall, leading to a spectrum of clinical manifestations including abdominal pain, nausea, vomiting, and diarrhea. Diagnosis of EE requires a high index of suspicion, often triggered by peripheral eosinophilia and confirmed through endoscopic biopsies demonstrating eosinophilic infiltrates in the intestinal mucosa. Management strategies predominantly involve corticosteroids to mitigate inflammation, with variable treatment responses observed among patients.</p><p>This case report presents a detailed description of a 20-year-old woman admitted to the emergency department with a history of chronic gastrointestinal symptoms over one year. She reported episodes of greenish vomiting, abdominal pain, and chronic diarrhea without fever, weight loss, or rash. Physical examination and initial laboratory tests revealed marked leukocytosis due to eosinophilia, peripheral blood smear confirming hypereosinophilia, and elevated immunoglobulin E levels. Abdominal ultrasound showed findings consistent with gastritis and duodenitis, corroborated by histopathological examination of biopsied tissues demonstrating eosinophilic infiltrates.</p><p>Treatment with methylprednisolone 20 mg/day resulted in significant improvement of symptoms. This case underscores the diagnostic challenge posed by EE and highlights the importance of considering this rare entity in the differential diagnosis of chronic gastrointestinal complaints with eosinophilia. Further research is warranted to enhance understanding of EE's pathophysiology and refine therapeutic approaches, particularly in cases resistant to conventional treatments.</p><p>This abstract emphasizes the critical role of early recognition and tailored management in optimizing outcomes for patients with EE, underscoring the need for increased clinical awareness and comprehensive diagnostic evaluation in similar clinical presentations.</p><p><b>PP-03-081</b></p><p><b>An interesting case of a vanishing polyp during colonoscopy due to ileocolic intussusception</b></p><p><b>Rasyiqatul Raminey</b> and Sumitro Kosasih and Norwani Dewi Basir and Kian Chai Lim and Vui Heng Chong</p><p><i>Gastroenterology Unit, Department of Medicine, RIPAS Hospital, Bandar Seri Begawan, Brunei Darussalam</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Altered bowel habit associated with abdominal pain and weight loss are warning symptoms and requires urgent evaluation given the increasing incidence of colorectal neoplasms including colorectal cancer. A 55-year-old man with a background history of hypertension, dyslipidemia, diet-controlled diabetes mellitus and thalassemia trait presented with a 2 months history of recurrent abdominal pain accompanied with loose stool. He also has weight loss of 3 kg over the 2 months. In between episodes of abdominal pain, his bowel was normal. On admission he had microcytic anemia (Hb 8.5 gm/dL and MCV 53.5). Upper gastrointestinal endoscopy was normal but colonoscopy was abandoned due to poor bowel preparation. A repeat colonoscopy interestingly showed a large elongated polyp seen upon reaching the ascending colon and in the attempt to search for the base of this polyp, the polyp vanished and was not seen on withdrawal. After the polyp vanished, the cecum and the ileocecal valve were seen and noted to be normal. A computed tomography scan confirmed intussusception of an ileal lipomatous polyp. The patient was referred to the surgical department for further management. This case highlights an interesting case of ileocolic intussusception that was reduced during colonoscopy.</p><p><b>PP-03-082</b></p><p><b>Endoscopic ampullectomy for a well to moderately differentiated ampullary adenocarcinoma: case report</b></p><p><b>Romielle Grazel Joi Ramos</b> and James Crisfil Fructuoso Montesa</p><p><i>Manila Doctors Hospital, Las Pinas City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Early ampullary cancers present with good prognosis. Pancreaticoduodenectomy has been standard treatment for ampullary cancers, but postoperative complications rate remains high. Therefore, raises a discussion on the role of local ampullectomy for early ampullary cancers.</p><p><b><i>Case Report:</i></b> A case of a 72-year-old male, known hypertensive and coronary artery disease (s/p PTCAx1) who presented with 1 week history of jaundice and tea-colored urine. On review of systems, patient also had unintentional weight loss of 10kg within 1 month. On evaluation, patient was noted to have generalized jaundice and icteric sclerae. Abdomen was soft, nondistended and nontender without palmar erythema and spider angiomata. CA 19-9 was noted to be 324x elevated (value: >12000). MRI and MRCP of abdomen revealed presence of a 1.6 cm periampullary soft tissue thickening, with moderate to severe upstream dilatation of the intrahepatic and extrahepatic ducts, and in the pancreatic duct. No liver metastasis and lymphadenopathies were noted. Snare ampullectomy performed via hot snare using Endo Cut Q alternate with soft coagulation. Ampullary mass retrieved measuring 2cm x 1cm x 1.5cm and sent for histopathology. Histopathology revealed ampullary adenocarcinoma. Monthly follow-up showed no recurrence of jaundice and decreasing bilirubin levels. Currently, patient is being treated with adjuvant chemotherapy.</p><p><b>PP-03-083</b></p><p><b>Post-cholecystectomy hepatic subcapsular biloma: a case report</b></p><p><b>Romielle Grazel Joi Ramos</b> and James Crisfil Fructuoso Montesa</p><p><i>Manila Doctors Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Hepatic subcapsular biloma is an uncommon yet serious complication after laparoscopic cholecystectomy, marked by bile collecting beneath the liver capsule. Even though it is rare, identifying this condition is essential due to its potential for causing morbidity.</p><p><b><i>Case report:</i></b> Case of a 65-year-old female with a medical history of hypertension and dyslipidemia. Patient is a known case of chronic calculous cholecystitis and underwent an uncomplicated laparoscopic cholecystectomy. Postoperatively, she developed intermittent crampy, generalized abdominal pain and constipation. Upon diagnostics, MRI and MRCP of Upper Abdomen revealed moderate-sized loculated fluid collection with some fluid-filled levels and mild surface enhancement of the liver along the right hepatic lobe, leading to the diagnosis of hepatic subcapsular biloma. The biloma was managed successfully with percutaneous drainage. This exhibits a rare complication managed effectively without the need for endoscopic retrograde cholangiopancreatography (ERCP).</p><p><b>PP-03-084</b></p><p><b>Strongyloidiasis induced duodenal ulcer: an unusual cause of dyspepsia</b></p><p><b>Kimchhay Ro</b><sup>1</sup> and Khang Chea<sup>2</sup></p><p><sup>1</sup><i>Calmette Hospital, Phnom Penh, Cambodia;</i> <sup>2</sup><i>Ekip medical centre, Phnom Penh, Cambodia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Strongyloidiasis is a common helminth infection in tropical and subtropical regions. Majority of infected patient are asymptomatic or experience dyspepsia. We report a case of strongyloidiasis induced duodenal ulcer in an immunocompetent patient presented with dyspepsia.</p><p><b><i>Case Description:</i></b> A 63-year-old non-smoker woman without underlying disease, presented with chronic epigastric pain with postprandial distress syndrome. There were no alarm feature. Initial treatment with proton pump inhibitors (PPI) and prokinetics yielded no improvement. Physical examination and laboratory test were unremarkable. She underwent upper endoscopy which showed a large ulcer surrounded by edematous mucosa at duodenal bulb. Histological examination identified strongyloides stercoralis larva with acute inflammatory cells infiltration and increase eosinophil. Stool examination also confirmed the presence of strongyloides stercoralis ova. The patient was treated with Ivermectin resulting in significant symptomatic improvement. Parasitological stools examination was negative 1 month later.</p><p><b>PP-03-085</b></p><p><b>Pseudotumor lesion of primary eosinophilic colitis: a rare endoscopic finding</b></p><p><b>Kimchhay Ro</b><sup>1,2</sup> and Khang Chea<sup>1</sup></p><p><sup>1</sup><i>Calmette Hospital, Phnom Penh, Cambodia;</i> <sup>2</sup><i>Ekip medical centre, Phnom Penh, Cambodia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Eosinophilic colitis is an uncommon disorder in adults with unclear pathogenesis. There is lack of consensus concerning diagnostic criteria. Colonoscopy were normal in more than 70% of case. This current study reported a rare form of primary eosinophilic colitis mimicking primary colon cancer</p><p><b><i>Case Description:</i></b> A 59-year-old man without underlying disease, presented 3-months history of abdominal pain with non-bloody diarrhea, and significant weight loss. Laboratory investigation showed elevated eosinophils (30.3%) otherwise normal. An abdominal computed tomography scan suggested a malignant mass at the hepatic flexure. Colonoscopy revealed a circumferential mass-like lesion at the hepatic flexure with multiple erosions surrounded by edematous mucosa, other segments of colon were normal. Histological examination reported hyperplastic glandular component with dense eosinophils. No dysplasia or malignancy seen. Patient was treated with 20mg/day of corticosteroid for 4 weeks then tapered progressively over 4 weeks. Significant clinical improvement within first week of treatment. Complete mucosal healing was confirmed by colonoscopy at 12 weeks follow-up.</p><p><b><i>Discussion:</i></b> Endoscopic characteristic of eosinophilic colitis is variable and non-specific. Mucosal lesions usually show nonspecific erythema or ulceration. Pseudotumor lesion is a rare endoscopic finding which could mimic primary colon cancer. Diagnostic establishment rely on the presence of prominent eosinophilic infiltration in colonic mucosa and hypereosinophilia.</p><p><b>PP-03-086</b></p><p><b>Novel ASD device closure for post esophagectomy leak: two cases with follow up, unique healing</b></p><p>Githma Wimalasena<sup>1</sup>, Randima Nanayakkara<sup>3</sup>, Janaka De Silva<sup>4</sup>, Ishan De Zoysa<sup>1,2</sup>, Nilesh Fernandopulle<sup>1,2</sup> and <b>Dulanja Senanayake</b><sup>1</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>Colombo North Teaching Hospital, Ragama, Sri Lanka;</i> <sup>4</sup><i>Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Anastomotic leaks are a notable complication seen in 5% -29% of patients undergoing esophagectomy for malignant indications. Teatment modalities include surgical repair, diversion and exclusion, endoscopic and conservative management. A novel approach involves the utilization of an atrial septal defect (ASD) closure devices, designed for cardiac applications, to seal off leakage sites post-oesophagectomy. Only few case reports of this approach are available in worldwide literature and longterm follow up data are scarce. We present two cases of succesful closure of post esophagectomy anastomotic leaks with an ASD device with 3 years follow up.</p><p><b><i>Case report:</i></b> Two male patients, aged 39 and 72 years underwent laparoscopic oesophagectomy for gastroesophageal junction tumour. Both patients were found to have anastomotic leakage within 10 days after surgery. Conventional management approaches failed and ASD closure devices (11mm and 15mm respectively) were successfully placed. Successful leak closure was observed in both cases. Follow up at 6 weeks with CECT revealed no recurrences and the devices were found to be dislodged. At 36 months post-procedure both patients were asymptomatic despite the devices being dislodged.</p><p><b><i>Discussion:</i></b> Despite limited use in gastrointestinal interventions, ASD closure devices offer minimally invasive, successful leak closure, potentially superior to conventional methods, providing a promising alternative, especially in refractory cases.</p><p>These cases underscores the efficacy of ASD closure devices in managing post-oesophagectomy leaks, highlighting their potential as valuable additions to leak management strategies. Further research into comparative effectiveness is warranted to delineate their optimal role in leak management post-oesophagectomy.</p><p><b>PP-03-087</b></p><p><b>Submucosal tunneling endoscopic resection (STER) for esophageal submucosal tumors: pioneering surgery in Sri Lanka</b></p><p>Githma Wimalasena<sup>1</sup>, Nilesh Fernandopulle<sup>1,2</sup> and <b>Dulanja Senanayake</b><sup>1</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Submucosal tumours (SMTs) are defined as masses originating below the mucosa and protruding into the gastrointestinal lumen. Often asymptomatic, SMTs larger than 3 cm should be resected according to the American Society for gastrointestinal endoscopy (ASGE) guidelines, while smaller non-malignant SMTs can be monitored. Traditional surgical methods are required for deeper lesions, but STER has emerged as a less invasive alternative, combining techniques from endoscopic submucosal dissection, and peroral endoscopic myotomy.</p><p><b><i>Case report:</i></b> A 52-year-old female with a 2-year history of dysphagia worsening over 5 months, was found to have a polypoidal growth at the gastroesophageal junction, of which histopathology confirmed a leiomyoma. Endoscopic ultrasound revealed an additional 42mm by 25mm submucosal lump in the mid-esophagus. The patient, classified as ASA class II, underwent STER under general anaesthesia. The procedure involved creating a submucosal tunnel, dissecting the lesion and removing it through the opening, which was closed by endoclips. There was minimal blood loss, no intraoperative complications and the patient recovered without issue, being discharged four days after surgery.</p><p><b><i>Discussion:</i></b> SMTs are increasingly detected due to advanced imaging and endoscopic techniques, with a reported incidence of 3%. Histological diagnosis calls for endoscopic ultrasound guided fine needle aspiration. Surgical resection although effective, is invasive with higher morbidity. STER, offering high en bloc resection rates and fewer complications, stands out as a safer alternative, with reduced hospital stays and adverse events.</p><p><b>PP-03-088</b></p><p><b>Sinistroposition left-sided gallbladder: A rare incidental intraoperative biliary tract variation during laparoscopic cholecystectomy</b></p><p>Githma Wimalasena<sup>1</sup>, Albert Joseph<sup>3</sup> and <b>Duminda Subasinghe</b><sup>2,3</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;</i> <sup>3</sup><i>University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Introduction:</i></b> The gallbladder is typically located in the hepatic segments IV and V. Left-sided gallbladder, defined as a gallbladder situated to the left of the falciform ligament, is a rare biliary anomaly. It can be, due to situs inversus, or true left-sided gallbladder which is further subdivided into medioposition and sinistroposition. True left-sided gallbladder is exceptionally rare, seen in only 0.04% of cholecystectomy patients. Laparoscopic cholecystectomy in these cases has a higher risk of bile duct injury compared to normally positioned gallbladders.</p><p><b><i>Case:</i></b> A 42-year-old obese (BMI=36.5 kg/m²) female presented with a five-month history of recurrent biliary colics. Her liver function tests were normal. Preoperative ultrasound revealed a solitary stone in the gallbladder neck. During laparoscopic cholecystectomy, a left-sided gallbladder (Type III) was found on segment IVB with the right hepatic artery running anterior to the common bile duct. There was an impacted stone in Hartmann’s pouch and two cystic arteries. Despite these challenges, a complete laparoscopic cholecystectomy was performed with a critical view of safety. The operation time was 120 minutes, and histopathology indicated chronic cholecystitis. The patient recovered uneventfully and was well three months post-surgery.</p><p><b><i>Discussion and conclusions:</i></b> Left-sided gallbladder is a rare anomaly with a prevalence of 0.1-0.7%. Conventional ultrasound often fails to detect it, surprising surgeons during laparoscopy. Successful removal requires modifications during surgery such as patient repositioning. Left-sided gallbladder, and its associated anormalies increase the risk of bile duct injury. Awareness of these anormalies is crucial for safe laparoscopic cholecystectomy.</p><p><b>PP-03-089</b></p><p><b>Gastric per-oral Endoscopic Myotomy for Treating Pylorospasm after Sleeve Gastrectomy</b></p><p><b>Li Wang</b><sup>1,2</sup>, Zu-Qiang Liu<sup>1</sup>, Quan-Lin Li<sup>1</sup> and Ping-Hong Zhou<sup>1</sup></p><p><sup>1</sup><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Endoscopy Center, Shanghai Geriatric Medical Center, Shanghai, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> A 44-year-old man was admitted because of acid reflux and heartburn for three years. The patient had undergone sleeve gastrectomy (SG) and tension-free repair of esophageal hiatal hernia three years and one year before admission, respectively. He was scheduled to undergo gastric per-oral endoscopic myotomy (G-POEM) after gastroscopy and gastric emptying examination, which showed esophagitis, pylorospasm (A), and gastric emptying disturbance.</p><p><b><i>Case Description:</i></b> After submucosal injection, a 2-cm mucosal incision was made 5cm above the antrum pylorus (B). After the submucosal tunnel was extended to 3cm below the pylorus, the muscle bundle was completely transected using the hybrid knife (C). After hemostasis with hot biopsy forceps, the mucosal entry on the stomach was closed with clips. After myotomy, the pylorus was remarkably enlarged, and the gastroscope passed smoothly without resistance (D). The patient recovered uneventfully and was discharged on the postoperative day 2. The patient did not experience any discomfort during the two-year postoperative follow-up.</p><p><b><i>Discussion:</i></b> Pylorospasm is a rare adverse event after SG. G-POEM after SG was more complicated and riskier than normal due to distortion of the gastric cavity and limited space for the procedure. This case provides the possibility of applying G-POEM to treat pylorospasm after SG.</p><p><b>PP-03-090</b></p><p><b>Conquering Challenging Common Bile Duct Stones – A Case Report</b></p><p><b>Louis Wang</b> and Andrew Boon Eu Kwek and James Weiquan Li and Kenneth Weicong Lin and Nicholas Chin Hock Tee and Tiing Leong Ang</p><p><i>Changi General Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Common bile duct (CBD) stones are prevalent and result in severe complications such as acute pancreatitis and cholangitis in untreated patients. Extraction of difficult CBD stones during endoscopic retrograde cholangiopancreatography (ERCP) is challenging.</p><p><b><i>Case Report:</i></b> A 43-year-old Malay male with no prior medical history presented with painless obstructive jaundice of three weeks, with serum total bilirubin 511 umol/L, direct bilirubin 461 umol/L, alkaline phosphatase 274 U/L. CT abdomen revealed marked biliary dilatation. MRCP confirmed two 18mm stones with distal CBD narrowing. A decision was made for ERCP with cholangioscopy-guided lithotripsy. Initial attempts using electrohydraulic lithotripsy (EHL) were ineffective in stone fragmentation. Laser lithotripsy (LL) was performed as a rescue procedure with successful stone fragmentation.</p><p><b><i>Discussion:</i></b> This case illustrates several characteristics of difficult CBD stones, including larger size (>1.5cm), increased number, hardness, impaction, and presence of distal ductal narrowing. While papillotomy with large-balloon dilation is advocated as first-line therapy for difficult CBD stones, cholangioscopy-assisted lithotripsy is increasingly shown to be more superior for CBD clearance with comparable safety. Although the efficacy of EHL is supposedly comparable to LL, single session fragmentation and ductal clearance rate is significantly higher with LL. Each laser impulse is more focused and of greater intensity than EHL. In calcified stones with failed EHL, LL can be successfully used as rescue therapy. It may be that LL, rather than EHL, should be preferentially used to treat calcified stones. This case highlights the considerations in managing difficult CBD stones, advocating for tailored approaches to enhance therapeutic outcomes.</p><p><b>PP-03-091</b></p><p><b>A rare cause of occult gastrointestinal (GI) bleeding: Small bowel metastases from malignant melanoma</b></p><p><b>Ying Wei</b><sup>1</sup>, Alyssa and Shin Yee Sim<sup>1</sup> and Christopher and Tze Wei Chia<sup>2</sup></p><p><sup>1</sup><i>Tan Tock Seng Hospital, Singapore, Singapore;</i> <sup>2</sup><i>Woodlands Health Campus, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Metastases of primary malignant melanoma to the small bowel occur in nearly 50% of patients but typically remain asymptomatic and undetectable until complications such as abdominal pain, bleeding, or anemia arise. Our case highlights an unusual instance of occult gastrointestinal bleeding due to small bowel metastases in a patient with primary vaginal melanoma.</p><p><b><i>Case Report:</i></b> A 48-year-old female with primary vaginal melanoma, diagnosed and resected 15 months prior, presented with severe symptomatic iron deficiency anemia (hemoglobin 5.6 g/L, ferritin 4 ug/L), weight loss, and abdominal bloating. Initial diagnosis and follow-up surveillance 6 months ago showed no distant metastases. A repeat Computed Tomography(CT) scan revealed new small bowel wall thickening and enlarged mesenteric and retroperitoneal lymphadenopathy. Antegrade double-balloon enteroscopy confirmed an ulcerated proximal jejunal mass with associated bleeding. Biopsies identified spindle and epithelioid cells with granular intracytoplasmic melanin pigment, and immunohistochemical stains confirmed recurrent metastatic melanoma. Her hemoglobin levels stabilized with intravenous iron and transfusion, and there was no overt GI bleeding. She was planned for systemic immunotherapy.</p><p><b><i>Discussion:</i></b> Although small bowel metastatic melanoma is a rare cause of GI bleeding, it should always be suspected in patients with a history of melanoma presenting with iron deficiency anemia and/or gastrointestinal symptoms. Early investigation in these cases can lead to prompt diagnosis and improved outcomes, especially with the evolving role of immunotherapy.</p><p><b>PP-03-092</b></p><p><b>Yellow Man: A Case Report About A Patient With Extremely High Bilirubin</b></p><p><b>Shiran Appuhamy</b> and Chamila Ranawaka</p><p><i>Colombo North Teaching Hospital, Ragama, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> This case report describes a 33-year-old male with a complex medical history including glucose-6-phosphate dehydrogenase (G6PD) deficiency, chronic haemolysis, multiple endocrine disorders and gall stone disease. He was presented with right upper abdominal pain, deep jaundice and fever. He was found to have common bile duct (CBD) stones. Endoscopic Retrograde Cholangiopancreatography (ERCP) successfully identified and cleared multiple CBD stones, resulting in significant clinical improvement.</p><p><b><i>Case description:</i></b> A 33-year-old male was admitted with acute right upper abdominal pain, deep jaundice, fever and pallor for five days duration. He is a known patient with G6PD deficiency with chronic haemolysis. He had a past history of cholecystectomy, hypoparathyroidism and hypogonadism. He had consanguineous parents.</p><p>Physical examination revealed deep jaundice, pallor, right hypochondrial (RHC) tenderness and splenomegaly. His blood pressure was 90/60 mmHg and pulse rate was 100 bpm. Initial laboratory tests were suggestive of biliary sepsis with cholestasis and ongoing haemolysis. Initial full blood count revealed pancytopenia. Other investigations were as follows;</p><p>Total bilirubin - 1205.9μmol/L</p><p>Direct bilirubin - 709.1 μmol/L</p><p>Indirect bilirubin - 496.8μmol/L</p><p>CRP - 23 mg/dL</p><p>Ultrasound scan abdomen showed CBD stones causing biliary obstruction with dilated CBD. He underwent urgent ERCP and three large stones were extracted.</p><p><b><i>Discussion:</i></b> Our patient already had chronic haemolytic anaemia due to G6DP deficiency and developed CBD stones over time. CBD stones were complicated with cholangitis, and it triggered acute haemolysis. Both acute haemolysis and cholestasis due to CBD obstruction could the possible explanation for the unusually high indirect and direct bilirubin levels.</p><p><b>PP-03-093</b></p><p><b>Endoscopic prevention of retrograde biliary drainage stent insertion for post-endoscopic retrograde cholangiopancreatography cholangitis</b></p><p><b>Yeong Heon Han</b>, Eui Joo Kim, Huapyong Kang and Yeon Suk Kim</p><p><i>Gachon University Gil Medical Center, Incheon, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Antibiotic prophylaxis may reduce the risk of infectious complications following endoscopic retrograde cholangiopancreatography (ERCP). However, the efficacy of endoscopic prophylactic measures for preventing post-ERCP cholangitis remains a subject of debate.</p><p><b><i>Methods:</i></b> In 2023, patients with choledocholithiasis who underwent ERCP were retrospectively reviewed at a single tertiary medical center. Data collected included patient demographics, diagnostic information, laboratory results, and ERCP-related details. Patients were classified as having post-ERCP cholangitis if they exhibited jaundice, fever, abdominal pain, and/or purulent drainage during a repeat ERCP. Bile duct stones were removed from all patients, and the clinical effect of a 5 Fr endoscopic retrograde biliary drainage (ERBD) stent after successful biliary stone removal was analyzed.</p><p><b><i>Results:</i></b> Data from 261 patients with choledocholithiasis were included. The mean age of the patients was 70.1 years, with 47.1% being women. All patients received prophylactic antibiotics prior to ERCP. Univariate analysis indicated that the 5 Fr ERBD stent was effective in reducing total bilirubin levels 4 hours after ERCP (P<0.023); however, there was no statistically significant effect on total bilirubin levels 24 hours after ERCP (P=0.105). There were 4 patients (1.3%) with post-ERCP cholangitis, and no differences were observed between patients who had the 5 Fr ERBD stent inserted and those who did not (1.7% VS 1.3%, P>0.999).</p><p><b><i>Conclusion:</i></b> In patients whose biliary stones have been completely removed and who are administered prophylactic antibiotics, the insertion of a 5 Fr ERBD stent does not confer additional prophylactic efficacy in reducing post-ERCP cholangitis.</p><p><b>PP-03-094</b></p><p><b>Diagnostic and Therapeutic Utility of SpyGlass Cholangioscopy in Biliary tract diseases: A single center experience</b></p><p><b>Leah Anne Legaspi</b> and Evan Ong</p><p><i>Metropolitan Medical Center, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Biliopancreatic ductal lesions pose diagnostic and therapeutic dilemma due to limited endoscopic access. SpyGlass cholangioscopy has provided an avenue for investigating these lesions through direct visualization. We aimed to review and summarize our single center experience.</p><p><b><i>Materials and Methods:</i></b> Fifty-nine (59) patients underwent SpyGlass cholangioscopy at our center between June 2022 to December 2023. Demographics, indications, technical and clinical success, difficulties, complications, and outcome were determined and assessed.</p><p><b><i>Results:</i></b> Fifty-nine patients underwent 69 SpyGlass cholangioscopies. The average age is 58 years (55±16), with 54% males. Diagnostic indication includes – ductal stricture in 41 cases (59%), ductal clearance documentation in 22 (32%), adjunct to SEMS insertion in 5 (6%), Holmium lithotripsy in 2 (2%) and intraductal fractured guidewire tip removal in 1 (1%). Majority 23 (60%) of strictures were hilar and common hepatic duct in location. Intraductal biopsy was employed in 32 strictures. Sixty-nine percent (25 biopsies) had benign histopathology while premalignant/malignant cases were seen in 31% (11 biopsies). The per-oral route was used in 65 cases (94%) and percutaneously in 4 cases (6%). Both technical and clinical success was achieved in 69 procedures (100%) and 40 procedures (100%) respectively. Difficulties are mainly inadequate visualization. Complication rate was 9% with mild to moderate severity, including cholangitis, pancreatitis, pain and malaise, all managed medically.</p><p><b><i>Conclusion:</i></b> SpyGlass cholangioscopy is a promising and a potentially useful tool in diagnosis and treatment of pancreaticobiliary diseases through direct visualization and targeted therapeutics. It has a high technical and clinical success with low periprocedural complication.</p><p><b>PP-03-095</b></p><p><b>A novel design of sphincterotome for patients with surgically altered gastric anatomy</b></p><p><b>Mai Sorachi</b> and Toshinobu Izumi</p><p><i>NHO Himeji Medical Center, Himeji City, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> It is difficult to control the orientation of a sphincterotome in patients with surgically altered gastric anatomy. Sphincterotomes available in this setting are rarely directed as intended. We designed sphincterotome to obtain the correct axis of the upside-down papilla. We report experiences about the new device.</p><p><b><i>Materials and Methods:</i></b> Sphincterotomy was performed in 10 cases with altered gastric anatomy (4 with Roux-en-Y reconstruction and 6 with Billroth II) from August 2021 to May 2024. Double-balloon endoscopes were used for Roux-en-Y and side-viewing duodenoscopes for Billroth II except a case using a double-balloon endoscope. We adopted a push-type sphincterotome. Its tip was curled by hand with the cutting wire allowed to run along outside of the bowed tip (Figure 1).</p><p><b><i>Results:</i></b> The new sphincterotomes steadily pointed to the proper alignment of the inverse papilla, resulting in completion of the procedure in all the patients. Side-viewing duodenoscopes tended to offer better views of the cutting site than forward-viewing balloon endoscopes. No significant complications including bleeding, pancreatitis and perforation were not found.</p><p><b><i>Conclusion:</i></b> When previous gastric surgery turns the anatomy of the papilla ‘upside down’, sphincterotomy could be performed steadily by using the new instrument.</p><p><b>PP-03-096</b></p><p><b>Metal stent versus plastic stent in endoscopic ultrasound-guided hepaticogastrostomy for unresectable malignant biliary obstruction</b></p><p><b>Daiki Yamashige</b>, Susumu Hijioka and Takuji Okusaka</p><p><i>National Cancer Center Hospital, Chuo-ku, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Whether metal stents (MSs), which are commonly used, or plastic stents (PSs) yield better outcomes for malignant biliary obstruction (MBO) in endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) is controversial. Thus, we aimed to compare outcomes of initial EUS-HGS performed with MSs or PSs.</p><p><b><i>Methods:</i></b> In this single-center retrospective study, we included patients (MS/PS groups: n=158/77) with unresectable MBO and performed multivariable analysis. The landmark date was defined as day 100 and used to evaluate the time to recurrent biliary obstruction (TRBO).</p><p><b><i>Results:</i></b> The clinical success rate was similar in both the groups. The mean total bilirubin percentage decrease at week 2 was significantly higher in the MS group than in the PS group (-45.5% vs. -27.3%, P=0.03). Median TRBO was significantly different between the MS and PS groups (183 and 92 days, respectively, P=0.048). TRBO within 100 days was comparable in both the groups but was significantly shorter only after 100 days in the PS group (adjusted hazard ratio [HR] 14.7, P<0.001). Adverse events (AEs) were significantly more common in the MS group than in PS group (24.7% vs. 9.1%, P=0.005), although AE risk was higher with PS in the cholangitis subgroup (Pinteraction=0.02). After endoscopic re-intervention (ERI), TRBO tended to be longer with revision PS (HR 0.64, P=0.26).</p><p><b><i>Conclusion:</i></b> Although MS provides early improvement of jaundice and long stent patency, PS provides a better safety profile, comparable stent patency until 100 days, and favorable stent patency after ERI. PS can also be an adequate and optimal palliation method in EUS-HGS.</p><p><b>PP-03-097</b></p><p><b>Efficacy and tolarability of the split and the standard bowel preparation regime, for colonoscopy</b></p><p><b>Nirodha Abeywardhana</b><sup>1</sup>, Kesara Rathnathunga<sup>2</sup>, Bandula Samarasinghe<sup>2</sup>, Priyantha Maduranga<sup>1</sup>, Dilan Jayarathne<sup>1</sup>, Kaushika Gunasekara<sup>2</sup> and Inosha Alwis<sup>2</sup></p><p><sup>1</sup><i>Teaching Hospital Peradeniya, Kandy, Sri Lanka;</i> <sup>2</sup><i>University of Peradeniya, Kandy, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Optimal bowel preparation is vital for the safety and efficacy of colonoscopy. The efficacy and tolerability of bowel preparation remains a problem worldwide. Most centers in Sri Lanka use 4L of Polyethylene glycol (PEG) interspersed with similar amount of clear liquids as a single dose. Some centers use split dose regime where 2L of PEG is prescribed in the evening prior to the study and the other 2L is prescribed next morning. To compare the efficacy and the tolerability of the split dose regime in comparison to the single dose regimen.</p><p><b><i>Materials and Methods:</i></b> An interventional study design was used. Patients awaiting colonoscopy were assigned in to the two arms.Those who had previous large bowel resections, diagnosed patients with bowel motility disorders were excluded. They were admitted day prior to procedure and administered the bowel preparation accordingly. One of the investigators inquired regarding compliance and the tolerability. During the procedure, the colonoscopist graded the bowel preparation in each colonic segment according to a validated scale, Boston Bowel Preparation Score (BBPS)</p><p><b><i>Results:</i></b> Fifty-four patients were in each group. Thirty-four out of them were females. Mann-Whitney U test was used to compare the distributions. Split dose regime showed a statistically significant difference in BBPS in all 3 segments of the colon and tolerability compared to continous regime (right: p=0.005, transverse: p<0.001, left: p<0.001)</p><p><b><i>Conclusions:</i></b> The split dose bowel preparation regime is superior to continuous regime in its efficacy of bowel preparation in all colonic segments and tolerability as well.</p><p><b>PP-03-098</b></p><p><b>Diagnostic value of artificial intelligence computer assisted diagnosis (cad eye function) for colorectal polyps</b></p><p><b>Hendra Asputra</b>, Achmad Fauzi, Hasan Maulahela and Cleopas Martin Rumende</p><p><i>Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To assess diagnostic value of AI CAD eye function for colorectal polyps</p><p><b><i>Material & Methods:</i></b> This study used a crosssectional design and was conducted at the RSCM Gastrointestinal Endoscopi Center in January-May 2024 on adult patients with of colorectal polyps.</p><p><b><i>Result:</i></b> A total of 60 patients with 100 polyps were involved in this study. The median age was 61.5 (20–76) years with 50% males and 50% females, the majority was Javanese (46.7%), 12% obese, 30% had IBD, 20% had diabetes, 13.3% had a family history of polyps, and 10% had a family history of colorectal cancer. The majority of polyps are 1-5 mm in size (56%),in the rectosigmoid (51%) and have a sessile morphology (75%). Based on the results of the examination, it was found that the AI CAD eye function examination had a sensitivity 79.17%, specificity 75%, PPV 89.06%, NPV 58.33% and accuracy of 78%. In polyps with dimminutive size, sensitivity 86.27%, specificity 60.00%, PPV 95.65%, NPV 30.00% and accuracy 83.93% were obtained. Meanwhile, in polyps with a nondimminutive size, sensitivity 61.90%, specificity 78.26%, PPV 72.22%, NPV 69.23%, and accuracy 70.45% were obtained. In sessile polyps, sensitivity was 81.54%, specificity 50.00%, PPV 91.38%, NPV 29.41% and accuracy 77.33%. Meanwhile, in non-sessile polyps, sensitivity was 57.14%, specificity 88.89%, PPV 66.67%. NPV 84.21%, and accuracy 80.00%</p><p><b><i>Conclusion:</i></b> AI CAD eye function examination has a high sensitivity value in dimmunitive and sessile polyps and high specificity in nondimmunitive and non sessile polyps.</p><p><b><i>Keywords:</i></b> AI, CAD eye function, colorectal polyps</p><p><b>PP-03-099</b></p><p><b>Characteristics of colorectal polyp patients at the gastrointestinal endoscopy center RSCM Jakarta period January-May 2024</b></p><p><b>Hendra Asputra</b></p><p><i>Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To assess the demographic features, risk factors, and characteristics of colorectal polyps at PESC Cipto Mangunkusumo Hospital from January to May 2024.</p><p><b><i>Material & Methods:</i></b> This descriptive-analytic study was conducted at the PESC RSCM from January to May 2024 on adult patients with colorectal polyps.</p><p><b><i>Result:</i></b> A total of 60 patients with 100 polyps were included in this study. Median (IQR) age was 61.5 years (20-76 years) most are within the range of 60-69 years (38.3%), gender are equal between men and women, which were 50% and 50%, the majority are of Javanese ethnicity (46.7%), followed by Batak and Chinese ethnicity each by 15%, a total of 12% of patients were obese, and 30% patients had a history of IBD, 20% had a history of diabetes mellitus, and 13.3% had a family history of polyps, and only 10% had a family history of colorectal cancer. The majority of polyps were 1-5 mm in size (56%), most located in the rectosigmoid (51%) and the majority of sessile morphology (75%).</p><p><b><i>Conclusion:</i></b> The majority were rectosigmoid sessile polyps, age 60-69, Javanese ethnicity, not obese, no history of IBD, no history of diabetes mellitus, no family history of polyps and colorectal cancer. The appearance of polyps in this study was most likely spontaneous.</p><p><b><i>Keywords:</i></b> demographics, risk factors, colorectal polyp characteristics.</p><p><b>PP-03-100</b></p><p><b>Computer-Aided Detection Versus Conventional Colonoscopy For Proximal Colon Polyps: Single Centre Randomized Tandem Colonoscopy Study</b></p><p><b>Nur Nadia Azman</b>, Rafiz Abdul Rani, Thevaraajan Jayaraman and Muhammad Ilham Abdul Hafidz</p><p><i>Universiti Teknologi MARA (UiTM), Sungai Buloh, Malaysia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Screening colonoscopy has had limited impact on improving outcomes for right-sided colorectal cancers. Computer-aided detection (CADe) was shown to improve detection of colorectal polyps. We aimed to evaluate adenoma detection rate (ADR) in the right colon using CADe vs conventional white light imaging (WLI).</p><p><b><i>Methodology:</i></b> This was a prospective randomized, tandem colonoscopy study conducted at Universiti Teknologi MARA. Screening colonoscopy patients aged 50-70 years were enrolled and randomly assigned to either first undergo CADe (CAD EYE®, Fujifilm Co, Japan) or WLI from caecum till splenic flexure, followed immediately by the other procedure in tandem and by a different endoscopist blinded from the first procedure.</p><p><b><i>Results:</i></b> Fourty four patients met the inclusion criteria with mean age of 63.6 ±6.2 years, majority female (56.8%) and ethnicly mainly Malay (68.2%). 52.3% and 56.8% patients scored Boston Bowel Prep Score of 3 at ascending colon and transverse colon respectively. The median (IQR) withdrawal time for CADe in the right colon was higher 8 (6,10) mins vs 7 (5,10) minutes. CADe had a higher ADR (27.3% vs. 20.5%, p <0.001) with higher total number of polyps detected (85 vs 72, p<0.001). CADe had higher Polyp Detection Rate (PDR 72.7% vs. 56.8%, p <0.0054). All polyps were <1cm. CADe detected more protruded polyps (Paris 0-Ip, 0-Is) (31vs 28, p<0.001) and flat polyps (Paris 0-IIa) (47 vs 37, p<0.001).</p><p><b><i>Conclusion:</i></b> CADe had a higher ADR and a higher number of total polyps detected in the right colon compared to conventional WLI colonoscopy.</p><p><b>PP-03-101</b></p><p><b>Topical Formalin Instillation vs. Argon Plasma Coagulation in Chronic Radiation Proctopathy: A Meta-analysis</b></p><p><b>Jan Bendric Borbe</b> and Eric Yasay</p><p><i>Philippine General Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Radiation proctopathy is a recognized complication of radiation therapy of pelvic tumors which often presents as rectal bleeding. While argon plasma coagulation (APC) is presently considered the treatment of choice for chronic radiation proctopathy (CRP), its use may be limited by availability especially in developing countries. The objective of this study is to assess the efficacy of topical formalin instillation compared to argon plasma coagulation in the management of CRP.</p><p><b><i>Materials and Methods:</i></b> Various electronic databases were searched from inception to December 2023. Randomized controlled trials comparing the effect of argon plasma coagulation and topical formalin instillation in the treatment of CRP were included in the study. The analysis performed using Revman version 5.4.</p><p><b><i>Results:</i></b> A total of 2 studies with a total of 57 patients were included in the quantitative analysis. Pooled analysis showed no statistically significant difference in the pre- and post-treatment change in hemoglobin between the formalin and the APC group 0.53 g/L (p = 0.11). In terms of the number of sessions to achieve the desired therapeutic endpoint, pooled analysis of the studies showed no statistical difference between the two groups with a mean difference of 0.06 days (p = 0.87) with no significant heterogeneity (I² 0%).</p><p><b><i>Conclusion:</i></b> The results suggest that topical formalin and APC are both effective treatments for chronic radiation proctitis with comparable rates of decreasing rectal bleeding as evidenced by improvement in post-treatment hemoglobin values. Similarly, the number of sessions required to achieve therapeutic success are comparable</p><p><b>PP-03-102</b></p><p><b>Lactulose vs. Polyethylene Glycol as Bowel Preparation Agent for Colonoscopy: A Metaanalysis</b></p><p><b>Jan Bendric Borbe</b>, Eric Yasay, Anton Elepano and Glen Anarna</p><p><i>Philippine General Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Lactulose is a non-absorbable disaccharide listed under the World Health Organization’s essential medicines. We aimed to summarize the evidence on the efficacy and tolerability of lactulose as an alternative bowel cleansing regimen to polyethylene glycol (PEG) for colonoscopy.</p><p><b><i>Materials and methods:</i></b> Literature search in databases and registers was done in April 2023. Included studies were randomized trials involving adult patients which compared lactulose to PEG for bowel preparation regardless of the indication for colonoscopy. Outcomes of interest were the quality of bowel preparation measured using ordinal scales, polyp detection, palatability of solution, and adverse events.</p><p><b><i>Results:</i></b> A total of four studies which comprised 536 patients were included in the analysis. Risk of bias is low in terms of participant selection, but there were issues on participant blinding and heterogeneity on the preparation of the interventions.</p><p>In all four studies, the quality of bowel cleanliness with lactulose was non-inferior to that of PEG. Odds of polyp detection favored lactulose over PEG (odds ratio 3.10; 95% confidence interval, 1.42 to 6.74) in one study. Meanwhile, palatability was also better in the lactulose group (odds ratio 0.10; 95% confidence interval 0.03 to 0.39) based on three studies. Usual adverse effects with use of lactulose were nausea, vomiting, abdominal pain, bloatedness, and dizziness.</p><p><b><i>Conclusion:</i></b> This systematic review showed that lactulose oral solution is both efficacious and well-tolerated for pre-colonoscopy bowel preparation. In settings where it is readily accessible, it may be a viable substitute bowel preparation agent to PEG for colonoscopy.</p><p><b>PP-03-103</b></p><p><b>Long-term oncological outcome of colonic stenting as bridge-to-surgery in malignant large bowel obstruction</b></p><p><b>Lee Yoong Elizabeth Chen</b>, Jun Ming Samuel Lim, Andrew Boon Eu Kwek, Mun Wang Lai, James Weiquan Li, Kenneth Weicong Lin and Tiing Leong Ang</p><p><i>Changi General Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Self-expandable metal stents (SEMS) as bridge-to-surgery (BTS) for malignant large-bowel obstruction (MBO) has increased in recent years, with high success-rates. However, the long-term oncological impact of colonic stenting has received mixed reviews, with concerns regarding metastatic recurrence-risk. We performed an audit in a tertiary centre examining outcomes over a 10-year period following SEMS insertion as BTS.</p><p><b><i>Materials and methods:</i></b> Data of patients who underwent SEMS insertion for MBO between September 2013-December 2023 were reviewed. Overall survival, metastases-free survival, metastatic occurrence, and association with AJCC staging and adjuvant chemotherapy were analysed.</p><p><b><i>Results:</i></b> Of 132 patients, we excluded 31 who had metastases at diagnosis and five who were lost to follow-up. Of the remaining 96, overall survival and disease-free survival were 90.9% and 66.2% respectively. Mean overall survival was 86.8 months (95% CI [75.1-98.6]), mean metastases-free survival 95.6 months (95% CI [88.7-102.5]). 24 patients (31%) developed distant metastases: 20 (83.3%) were AJCC stage 3, 11 (45.8%) completed adjuvant chemotherapy, 3 (54.1%) declined or had attenuated doses. Using Kaplan-Meier estimates, the association between lack of completion of adjuvant chemotherapy and metastatic occurrence was insignificant (P-value 0.114). Metastatic recurrence was positively associated with higher AJCC staging (P-value 0.037).</p><p><b><i>Conclusion:</i></b> Rate of occurrence of distant metastases was similar to prior reported literature. Majority who developed metastases were AJCC stage 3 who declined or had attenuated adjuvant chemotherapy. The data suggests that colonic stenting as BTS does not worsen long-term oncological outcome. Metastatic recurrence was related to disease-stage and lack of effective adjuvant chemotherapy.</p><p><b>PP-03-104</b></p><p><b>Use Of Gel Immersion Technique In Colonic Diverticular Bleeding</b></p><p><b>Ronell Lee</b>, Patricia Te and Willy Alba</p><p><i>Chinese General Hospital And Medical Center, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives</i></b></p><p>• To demonstrate the consistency of the gel</p><p>• To compare the visibility of water from the gel once mixed with fresh blood</p><p>• Demonstrate the effectiveness of gel immersion technique in active colonic diverticular bleeding</p><p><b>Materials and methods</b></p><p><b><i>Materials:</i></b> Viscoclear gel #1, 50cc Syringe filled with fresh blood #1, Liter Glass bottles #2, Water</p><p><b><i>Methods:</i></b> Two different 1.0 Liter glass bottles were filled with same amount of water in one bottle and viscoclear in another bottle. Fresh blood in a 50cc syringe was poured on both bottles consecutively. The mixtures were observed up to 1 hour.</p><p><b><i>Clinical:</i></b> An 87 year old male with a Lower Gastrointestinal bleeding from an ascending colon diverticulosis underwent colonoscopy. Gel was flushed to the bleeding site.</p><p><b><i>Results:</i></b> Water immediately mixed with blood upon contact. Gel appeared to hold and prevent blood from scattering throughout the gel medium. After 1 hour, water appeared to mix thoroughly with blood while the blood in the gel did not scatter.</p><p><b><i>Clinical:</i></b> The bleeding lesion was easily identified because the bleeding was slowly oozing from the site and did not scatter. A hemoclip was deployed on the bleeding site. Hemostasis was achieved afterwards.</p><p><b>Conclusion</b></p><p>• The gel immersion technique is an effective method of improving endoscopic visualization during active colonic diverticular bleeding</p><p><b>PP-03-105</b></p><p><b>Improving Colorectal Polyp Size Measurement with Artificial Intelligence: Prospective, Preliminary Comparison of Accuracy</b></p><p><b>Xiangyu Sui</b><sup>1</sup>, Chenglong Wang<sup>2</sup>, Xinxin Huang<sup>1</sup>, Wenlong Li<sup>1</sup>, Zhaoshen Li<sup>1</sup>, Shengbing Zhao<sup>1</sup> and Yu Bai<sup>1</sup></p><p><sup>1</sup><i>Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, China;</i> <sup>2</sup><i>Department of Colorectal Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Accurate polyp size measurements are crucial for risk stratification, selection of polypectomy techniques, and follow-up interval assignments. This study aimed to compare the accuracy of a deep learning-based system (EndoDASS) to visual estimation, non-calibrated tools (biopsy forceps or snares), and calibrated forceps in measuring polyp size during real-time colonoscopies.</p><p><b><i>Materials and methods:</i></b> Visual estimation, non-calibrated tools, calibrated forceps-based estimation, and EndoDASS were compared using a 3D-printed colorectal model with 10 polyps of defined sizes. Ten endoscopists estimated polyp sizes visually, using non-calibrated tools, and calibrated tools. Repeated measurements were performed one week apart to evaluate measurement consistency. Additionally, measurements of 24 colorectal polyps during clinical colonoscopies were compared. The primary outcome was relative accuracy in polyp size measurement.</p><p><b><i>Results:</i></b> EndoDASS exhibited a relative size measurement accuracy of 96.3%, significantly surpassing that of visual estimation (75.7%, P<0.001), non-calibrated tools (86.4%, P<0.001), and calibrated forceps (80.8%, P<0.001) in the 3D-printed model. EndoDASS demonstrated superior measurement consistency, with an intraclass correlation coefficient of 0.999 for two repeated measurements, surpassing visual (0.577, P<0.001), non-calibrated tools (0.807, P<0.001), and calibrated forceps (0.774, P<0.001). During real-time colonoscopies, EndoDASS achieved a significantly higher relative accuracy (86.7%), compared to visual (54.1%, P<0.001) and non-calibrated tools (69.2%, P=0.008). EndoDASS demonstrated a significantly higher percentage of accurate size measurements within 25% of the true size (95.8%), compared to visual estimation (45.8%, P<0.001) and non-calibrated tools (54.2%, P<0.001).</p><p><b><i>Conclusion:</i></b> The novel deep learning-based system, EndoDASS, demonstrated significantly higher accuracy in measuring colorectal polyp size compared to other common sizing methods.</p><p><b>PP-03-106</b></p><p><b>Results of colonoscopic resection of large ( ≥ 2 cm) pedunculated polyps with endoloop-assisting device</b></p><p><b>Thuy Tran Ngoc</b><sup>1</sup> and Tuyet Le Thi Anh<sup>2</sup></p><p><sup>1</sup><i>Thai Nguyen Medical College, Thai Nguyen, Viet Nam;</i> <sup>2</sup><i>108 Military Central Hospital, Ha Noi, Viet Nam</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate efficacy and safety of cutting large pedunculated colorectal polyps using an Endoloop-assisting device.</p><p><b><i>Materials and Methods:</i></b> Prospective study on 88 patients with pedunculated colorectal polyps ≥ 2 cm in size. These polyps were ligated with an Endoloop and cut using an electric snare through flexible endoscopy in 108 Military Hospital, Ha Noi, Viet Nam, from April 2023 to June 2024.</p><p><b><i>Results:</i></b> The average age of the study patient group was 59.8 ± 14.4 years old. The male/female ratio was 2.4/1. The number of polyps ≥ 2 cm was 88 polyps in 88 patients. The main location was in the left colon (48.9%). The average size of polyps was 2.5 ± 0.7 cm. The average length of the polyp stalk was 1.2 ± 0.6 cm. The average polyp stalk diameter was 1.2 ± 0.3 cm. Successful polyp removal results were 100%. The average polyp removal time was 21.2 ± 8.8 minutes. Post-resection complications: secondary bleeding rate was 2.4% due to enlarged blood vessels feeding the polyp, additional bleeding was stopped with clips, post-polyp removal abdominal pain rate was 20%. The rate of patients staying in hospital < 24 hours after polyp removal was 84.0%.</p><p><b><i>Conclusion:</i></b> Removing pedunculated colorectal polyps ≥ 2 cm with the support of Endoloop is an effective, safe method with few serious complications.</p><p><b>PP-03-107</b></p><p><b>Lemmel’s syndrome as uncommon cause of obstructive jaundice : a rare case report</b></p><p><b>Iqbal Habibie</b><sup>1</sup>, Vesri Yoga<sup>2</sup>, Arnelis<sup>2</sup> and Saptino Miro<sup>2</sup></p><p><sup>1</sup><i>Faculty of Medicine, Andalas University, Padang, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Andalas University, Padang, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Lemmel’s syndrome is obstructive jaundice without choledocholithiasis or pancreaticbilliary tumors. This syndrome was first reported in 1934 by Lemmel, who described the presence of periampullary duodenal diverticulum (PAD). Periampullary duodenal diverticulum (PAD) is usually asymptomatic, and is found incidentally in 22% of the population, only 5-10% of patients show complications. This syndrome of obstructive jaundice of duodenal diverticulum is called Lemmel’s syndrome.</p><p><b><i>Case Description:</i></b> A 64-year-old woman presents to the emergency room with jaundice and abdominal pain. Abdominal ultrasound showed dilatation of the central intrahepatic bile duct without a demonstrable cause of obstruction. An ERCP decision is taken, in which to confirm the diagnosis.</p><p><b><i>Discussion:</i></b> Endoscopic retrograde cholangiopancreatography (ERCP) showed giant diverticula in the second part of the duodenum. Cholangiography showed dilatation of the CBD without a filling defect, indicating the absence of stones or tumors in the bile duct. After stent placement, all symptoms improved. Recognizing this condition is important because misdiagnosis can lead to mistreatment and delays in therapy. Lemmel’s syndrome should always be included in the differential diagnosis of obstructive jaundice if PAD is present.</p><p><b><i>Keywords:</i></b> Lemmel’s syndrome, PAD, ERCP</p><p><b>PP-03-108</b></p><p><b>Hybrid Peripheral Nerve Sheath Tumor Detected by Endoscopic Full-Thickness Resection for Gastric Submucosal Tumors</b></p><p><b>Mai Fukuda</b></p><p><i>Department Of Endoscopythe Jikei University School Of Medicine, Tokyo, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Case Description:</i></b> Hybrid peripheral nerve sheath tumors are exceedingly rare, especially those located in the stomach. Here, we present a case of an enlarged gastric submucosal tumor (SMT) treated with endoscopic full-thickness resection (EFTR), leading to a pathology diagnosis of a hybrid peripheral nerve sheath tumor. A 50-year-old woman presented with a 10-mm SMT on the posterior wall of the upper gastric curvature. The tumor, contiguous with the fourth layer of the gastric wall, appeared as a low-homoechoic area on endoscopic ultrasound (EUS). Following a boring biopsy, the tumor tested positive for S100 and SOX10 protein immunostaining, initially diagnosed as a granular cell tumor. However, six months later, the tumor had grown to 15 mm in size, prompting endoscopic resection. Due to the tumor's involvement of the muscle layer, the procedure was performed under general anesthesia, with the possibility of surgical conversion. Despite the challenge posed by the large, kyphotic lesion, resection was successfully achieved across all layers contiguous to the muscle layer. Post-resection, the ulcer, including the perforated area, was closed with clips. The patient's postoperative course was favorable, and she was discharged on the 7th day. Pathology results confirmed the diagnosis of hybrid peripheral nerve sheath tumor. EFTR was not only effective as a treatment but also served as a diagnostic method. This case suggests that EFTR may be an effective diagnostic and therapeutic option for small subepithelial lesions (SELs).</p><p><b>PP-03-109</b></p><p><b>Endoscopic submucosal dissection after chemoradiotherapy for locally advanced rectal cancer</b></p><p><b>Chun-Sheng Shen</b><sup>1,2,3,4</sup>, Ping-Ju Wu<sup>2,3,5</sup> and Ching-Chun Li<sup>1,2,3</sup></p><p><sup>1</sup><i>Kaohsiung Municipal Siaogang Hospital, Kaohsiung City, Taiwan;</i> <sup>2</sup><i>Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan;</i> <sup>3</sup><i>Kaohsiung Medical University, Kaohsiung City, Taiwan;</i> <sup>4</sup><i>Kaohsiung Medical University Gangshan Hospital, Kaohsiung City, Taiwan;</i> <sup>5</sup><i>Graduate Institute of Clinical Medicine Kaohsiung Medical University, Kaohsiung City, Taiwan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> We report an elderly case with locally advanced rectal cancer underwent chemoradiotherapy then resected by endoscopic submucosal dissection.</p><p><b><i>Case Description:</i></b> An 85-year-old male with rectal cancer, 2cm from anal verge, adenocarcinoma, grade 2, cT2N0M0, stage I, who underwent 3 cycles of chemotherapy with FOLFOX6 during October 2023 till November 2023 and radiotherapy (tomotherapy) during October 2023 till December 2023, total dose 5000cGy/25fr. He later took oral form Xeloda from December 2023 till May 2024. Follow-up of colonoscopy revealed regression of the rectal cancer in April 2024. As he has coronary artery disease with stenting in 2020 and heart failure, he is not a surgical candidate. After multiple-discipline team discussion, he underwent endoscopic submucosal dissection (ESD) with only local anesthesia. The ESD expanded 40 minutes, the pathology of specimen consisted of one tissue fragment measuring 4.2 x 2.0 cm in size with only adenomatous hyperplasia and no residual adenocarcinoma. There was no intra-procedure and delayed complication. He discharged safely 3 days later and has been followed up in the out-patient clinic recently.</p><p><b><i>Discussion:</i></b> ESD for locally advanced rectal cancer is an option as local excision rather than wait-and see strategies. For elderly patient who is vulnerable to traditional surgery, this procedure might be the last resort for providing pathology result of proper treatment and might be a curative intervention.</p><p><b>PP-03-110</b></p><p><b>Diagnostic Dilemma of Biliopancreatic Contrast-Enhanced Harmonic Endoscopic Ultrasonography</b></p><p><b>Takahiro Abe</b> and Masayuki Kato and Nana Shimamoto and Keisuke Kanazawa and Masafumi Chiba and Kimio Isshi and Kazuki Sumiyama</p><p><i>The Jikei University School Of Medicine, Tokyo, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The effectiveness of contrast-enhanced harmonic endoscopic ultrasonography (CH-EUS) in the biliopancreatic region is limited because it relies heavily on the endoscopist's skill. This study aimed to validate CH-EUS's efficacy in clinical practice.</p><p><b><i>Methods:</i></b> From January 2018 to March 2019, 301 patients who underwent CH-EUS were enrolled. The diagnostic performance of CH-EUS was compared with dynamic computed tomography (CT), magnetic resonance imaging (MRI), and all combined methods using a Bonferroni correction. Multiple logistic regression was used to identify diseases where CH-EUS matched the final diagnosis.</p><p><b><i>Results:</i></b> No significant differences in diagnostic performance were found among dynamic CT, MRI, and CH-EUS (P = 1.00). However, combining all methods significantly improved diagnostic performance (P < 0.001). Only intraductal papillary mucinous neoplasm (IPMN) showed significant agreement with the final diagnosis (P = 0.006).</p><p><b><i>Conclusions:</i></b> CH-EUS can diagnose IPMN in clinical practice. For other biliopancreatic diseases, a comprehensive diagnosis using multiple methods is needed, rather than relying on CH-EUS alone.</p><p>This research has been published in the (Diagnostics 2022).</p><p><b>PP-03-111</b></p><p><b>Endoscopic Ultrasound Guided Gastroduodenal Artery Embolization- A Case of Refractory Bleeding Ampullary Carcinoma</b></p><p><b>Deborah Chia Hsin Chew</b><sup>1,4</sup>, James Emmanuel<sup>2</sup>, Wing Hang Woo and Nisha Puvanendran<sup>1</sup> and Tiong See Lee<sup>3</sup></p><p><sup>1</sup><i>National University of Malaysia Medical Centre, Cheras, Malaysia;</i> <sup>2</sup><i>Queen Elizabeth Hospital, Kota Kinabalu, Malaysia;</i> <sup>3</sup><i>Selayang Hospital, Batu Caves, Malaysia;</i> <sup>4</sup><i>GUT Research Group, Cheras, Malaysia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>A 62-year-old lady with a background of ampullary cancer with liver metastasis post hepaticogastrostomy due to failed ERCP and palliative chemotherapy presented to the emergency unit with malena. She was in hypovolemic shock with a blood pressure of 85/46 mmHg and a heart rate of 112 beats per minute. Haemoglobin level was 3.5 g/dL. Oesophagogastroduodenoscopy performed revealed a bleeding ampullary tumour. APC temporarily achieved hemostasis, however, she developed a recurrence of Upper GI bleeding the following day. Computed tomography angiography of the abdomen showed dense material within the duodenum in keeping with blood clots however there was no active bleeding identified. Another session of APC was performed unsuccessfully. An EUS was performed immediately to localize the feeding vessel. The proximal aspect of the Gastroduodenal artery was identified from D1 of duodenum via EUS guided Color-doppler and was punctured with a 22G needle followed by injection of 0.5cc histoacryl glue, monomeric n-butyl-2-cyanoacrylate into the vessel. No further Doppler signal was noted from the GDA post embolization and haemostasis was achieved evidenced on the endoscopic view of the echoendoscope. Patient tolerated the procedure well with no evidence of complications. There were no further episodes of rebleed following the embolization procedure and the patient was discharged after 2 days. EUS-guided injection of sclerotherapy offers a less invasive yet effective approach for achieving haemostasis in cases of refractory bleeding AC. This method can be considered as a potential alternative when standard treatments have failed or are not suitable for the patient.</p><p><b>PP-03-112</b></p><p><b>Early Chronic Pancreatitis Findings by Endoscopic Ultrasonography (EUS) in Asymptomatic Patients with Pancreas Divisum</b></p><p><b>Masatsugu Nagahama</b>, Yuichi Takano, Fumitaka Niiya, Tetsushi Azami, Jun Noda and Naoki Tamai</p><p><i>Division of gastroenterology, Showa University Fujigaoka Hospital, Yokohama, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Pancreatic divisum (PD) is a congenital malformation associated with chronic and recurrent acute pancreatitis. Although PD often presents asymptomatically, the extent to which early chronic pancreatitis (ECP) changes occur in asymptomatic patients with PD remains unclear. This study aimed to evaluate endoscopic ultrasonography (EUS) findings indicative of ECP in asymptomatic patients with PD and investigate the relationship between these findings and background factors, such as age, sex, main pancreatic duct diameter, and alcohol intake.</p><p><b><i>Materials and Methods:</i></b> We retrospectively analyzed 17 asymptomatic patients diagnosed with PD at the Showa University Fujigaoka Hospital between January 2016 and May 2024. EUS was used to assess the pancreatic parenchyma and ductal features, and the findings were classified according to the Rosemont Classification (RC) and the Japan Pancreas Society (JPS) 2019 criteria for ECP (JDCECP2019). Statistical analyses were performed to examine the association between EUS findings and patient background factors.</p><p><b><i>Results:</i></b> EUS findings of ECP were observed in eight of 17 asymptomatic patients with PD (47%) according to both the RC and JDCECP2019 criteria. Hyperechoic foci without shadowing or strands were the most common findings, present in 82% of patients. No significant associations were found between EUS findings and background factors of age, sex, main pancreatic duct diameter, or alcohol intake.</p><p><b><i>Conclusions:</i></b> A considerable proportion of asymptomatic patients with PD exhibited EUS findings suggestive of early chronic pancreatitis. These findings indicate that early changes in chronic pancreatitis may be accompanied by asymptomatic patients with PD.</p><p><b>PP-03-113</b></p><p><b>Endoscopic ultrasound (EUS) guided diagnosis of pancreatic lesions – Single center Experience in Sri Lanka</b></p><p>Githma Wimalasena<sup>1</sup>, Dulanja Senanayake<sup>1</sup>, Jesuthasan Mithushan<sup>1</sup>, <b>Duminda Subasinghe</b><sup>2,3</sup>, Sivasuriya Sivaganesh<sup>2,3</sup>, Rohan Siriwardana<sup>4</sup>, Aloka Pathirana<sup>5</sup>, Suchintha Thilakarathna<sup>4</sup>, Malith Nandasena<sup>5</sup>, Harshima Wijesinghe<sup>6</sup>, A Priyani<sup>6</sup> and Nilesh Fernandopulle<sup>1,2</sup></p><p><sup>1</sup><i>Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;</i> <sup>2</sup><i>University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;</i> <sup>3</sup><i>Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;</i> <sup>4</sup><i>Colombo North Teaching Hospital, Ragama, Sri Lanka;</i> <sup>5</sup><i>Colombo South Teaching Hospital, Kalubowila, Sri Lanka;</i> <sup>6</sup><i>Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> EUS-FNA allows histopathological diagnoses of pancreatic tumours to be made when radiological imaging is inconclusive or when histological conformation is required before neoadjuvant therapy for inoperable pancreatic cancers. Previously, these patients were subjected to surgery or chemotherapy solely based on radiology and tumour markers which have their own inherent risks. We aimed to assess the diagnostic accuracy of EUS-FNAB in patients with suspected pancreatic tumours where histological confirmation was required.</p><p><b><i>Material and Methods:</i></b> A total of 297 patients with suspected pancreatic masses were referred for EUS to University Surgical Unit, National Hospital of Sri Lanka ,where EUS-FNAB was done.</p><p><b><i>Results:</i></b> The mean age of the population was 54.91 years (SD – 15.07 years) and 156 (52.5%) were males. A majority of the lesions (161,54.2%) were solid and found in the head (57.5%). Complications included pancreatitis and infection in 2 patients each (0.67%) which were managed medically. 115 (61.2%) were malignanct lesions, 37 (19.7%) inflammatory and 36 (19.1%) benign. Out of the malignant lesions, 68 (59.1%) were adenocarcinomas and 19 (16.5%) neuroendocrine tumors. Solid masses were significantly associated with malignancy (p<0.001). Adenocarcinomas were more likely to be seen in males (p=0.013), and in the head of the pancreas (p=0.009).</p><p><b><i>Conclusion:</i></b> In an era where personalized medicine is advocated, EUS-FNAB of pancreatic neoplasms allows effective and accurate pathological diagnoses to be made permitting oncologists to provide personalized oncotherapy to patients, which cannot be achieved by imaging alone. Single use of needles and onsite histopathology (ROSE) will further improve diagnostic yield.</p><p><b>PP-03-114</b></p><p><b>Comparative study on clinical changes in patients taking bowel cleansers</b></p><p><b>Young Cheon Ra</b></p><p><i>Division of Gastroenterology And Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> There were not many studies that compared blood test results with each bowel cleansers in real world. In addition, several studies have been published on safety and efficacy in elderly patients, but no conclusions have been reached. so it was necessary to check what kind of results actually come out in the real world. In this regard, we attempted to conduct a retrospective comparative study obtained from patients within each group when using oral sulfate tablets compared to previously existing bowel cleansing agents.</p><p><b><i>Materials and methods:</i></b> Data collection was prospectively conducted on patients aged 18 to 80, who visited for colonoscopy, who have taken OST or Low PEG or Very low PEG over the past year (Jan 2022 - Jan 2023). The evaluation variables are electrolyte changes, BUN/Cr, BBPS score, adenoma detection rate, adverse effects, and survey after colonoscopy.</p><p><b><i>Results:</i></b> There were no significant differences in baseline characteristics and no significant difference in adenoma detection rate. there were no life-threatening side effects. When patients took Oral Sulfate Tablet , they often took them all, and it was found that taking them was much easier.</p><p><b><i>Conclusion:</i></b> In real world, compared to classical bowel cleansing agents such as Low PEG and Very low PEG, OST’s efficacy and safety is not inferior. Based on this study’s survey, if this is a group of patients who had difficulty with bowel preparation due to previous PEG, and additional colonoscopy is required, it would be a good idea to consider OST for the next examination.</p><p><b>PP-03-115</b></p><p><b>Feasibility of a novel unassisted single-channel transcolonic endoscopic appendectomy for the treatment of appendiceal lesions</b></p><p><b>Li Wang</b></p><p><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <i>Endoscopy Center, Shanghai, Geriatric Medical Center, Shanghai, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Transcolonic endoscopic appendectomy (TEA) is rapidly evolving and has been reported as a minimally invasive alternative to appendectomy. We aimed to characterize the feasibility and safety of a novel unassisted single-channel TEA.</p><p><b><i>Materials and Methods:</i></b> We retrospectively investigated 23 patients with appendicitis or appendiceal lesions who underwent TEA from February 2016 to December 2022. We collected clinicopathological characteristics, procedure-related parameters, and follow-up data and analyzed the impact of previous abdominal surgery and traction technique.</p><p><b><i>Results:</i></b> The mean age was 56.0 years. Of the 23 patients with appendiceal lesions, fourteen patients underwent TEA, and nine underwent traction-assisted TEA (T-TEA). Eight patients had previous abdominal surgery. The En bloc resection rate was 95.7%. The mean procedure duration was 91.1± 45.5 minutes, and the mean wound closure time was 29.4 ± 18.6 minutes. The wounds after endoscopic appendectomy were closed with clips (21.7%) or a combination of clip closure and endoloop reinforcement (78.3%), and the median number of clips was 7 (range, 3-15). Three patients (13.0%) experienced major adverse events, including two delayed perforations (laparoscopic surgery) and one infection (salvage endoscopic suture). During a median follow-up of 23 months, no residual or recurrent lesions were observed, and no recurrence of abdominal pain occurred. There were no significant differences between TEA and T-TEA groups and between patients with and without abdominal surgery groups in each factor.</p><p><b><i>Conclusions:</i></b> Unassisted single-channel TEA for patients with appendiceal lesions has favorable short- and long-term outcomes. TEA can safely and effectively treat appendiceal disease in appropriately selected cases.</p><p><b>PP-03-116</b></p><p><b>Management of Delayed Bleeding of Peroral Endoscopic Myotomy: A retrospective single-center analysis and Brief Meta-analysis</b></p><p><b>Li Wang</b><sup>1,2</sup>, Shao-bin Luo<sup>1</sup>, Zu-Qiang Liu<sup>1</sup>, Quan-Lin Li<sup>1</sup> and Ping-Hong Zhou<sup>1</sup></p><p><sup>1</sup><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Endoscopy Center, Shanghai Geriatric Medical Center</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Delayed bleeding is a rare but important major adverse event (mAE) after endoscopic submucosal tunneling procedures, which is scarcely reported. We aimed to characterize the clinical characteristics of delayed bleeding and provide better management of this mAE.</p><p><b><i>Materials and Methods:</i></b> From August 2010 to October 2022, we reviewed 3,852 patients with achalasia receiving peroral endoscopic myotomy (POEM) and 1,937 patients with upper GI tumors receiving submucosal tunneling endoscopic resection (STER). Among these, records of 22 patients (15 POEM, 7 STER) with delayed bleeding were collected. Clinical characteristics, treatment, and outcomes of delayed bleeding were analyzed.</p><p><b><i>Results:</i></b> The mean age was 43.6 years. Ten patients (45.5%) were intratunnel bleeding, seven (31.8%) were intratunnel bleeding accompanied by mucosal bleeding, and 5 (22.7%) were mucosal bleeding. The most common accompanied symptoms were hematemesis, fever, and melena. The most common accompanied mAEs were fistula, pulmonary inflammation, and pleural effusion with atelectasis. The mean duration from ESTP to endoscopic intervention was 5.3±4.9 days. Active bleeding was identified in 21 patients (95.5%). The bleeding was successfully controlled by electrocoagulation (19 cases), endoscopic clipping (6 cases), and Sengstaken-Blakemore tube insertion (3 cases) and no patient required surgical intervention. The mean hemostatic procedure duration was 61.8±45.8 minutes. The mean post-bleeding hospital stay was 10.0±6.2 days. A brief meta-analysis of previous studies showed the pooled estimate delayed bleeding rate after POEM, STER, and G-POEM was 0.4%.</p><p><b><i>Conclusions:</i></b> Delayed bleeding is uncommon and could be effectively managed by timely emergency endoscopic procedures without requiring subsequent surgical interventions.</p><p><b>PP-03-117</b></p><p><b>GERD-A Novel predictor,In hoarseness(Of voice) patients with assessment of impedance pH and acoustic voice analysis</b></p><p><b>Jay Chudasama</b></p><p><i>Topiwala National Medical College, Mumbai, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>A study of reflux and voice disorders suggests that up to 55% of patients with hoarseness have laryngopharyngeal reflux. Exact incidence of gastroesophageal reflux disease(GERD) is not known in these sets of patients. Relation of acoustic parameters with GERD is also not studied well. Aim of the study was to investigate frequency of GERD in patients with hoarseness of voice and to study acoustic voice parameters & 24-hour impedance pH-metry finding in hoarseness with GERD patients.</p><p><b><i>Methods</i></b>: 406 patients identified with hoarseness of voice of more than 6 weeks in ENT OPD, speech clinic and gastroenterology OPD combined.On basis of history, indirect laryngoscopy and speech parameters assessment ,374 patients were excluded for smoking, neurological, infectious causes of hoarseness.Remaining 32 patients underwent upper GI scopy, oesophageal manometry and 24-hour pH-metry.GERDwas diagnosed on basis of Lyon consesus.</p><p><b><i>Results:</i></b> After exclusion,32 individuals with hoarseness of voice were evaluated further. Most common associated typical symptom were regurgitation (67%) and heart burn (50%). Mean DeMeester Score was 14.20 ±16.6. Mean AET was 3.96± 5.05 %. Mean No.Of Reflux Episodes 99± 84. Total 19 individuals diagnosed with GERD by using endoscopy(3) and impedance pH analysis(16) criteria.</p><p>12 acoustic parameters were studied in relation to GERD.</p><p>On univariate analysis, Jitter %, Absolute jitter, Pitch perturbation quotient, Shimmer were significantly associated with GERD (p<0.05). On multivariate analysis, Absolute jitter and Shimmer( P < 0.05) were independent predictors for GERD.</p><p><b><i>Conclusions:</i></b> GERD was cause of idiopathic hoarseness of voice in 7.88 % patients.Speech parameters absolute Jitter and Simmer(db) are independent predictors of GERD.</p><p><b>PP-03-118</b></p><p><b>Hiccups-you can’t think this one !</b></p><p><b>Jay Chudasama</b></p><p><i>Topiwala National Medical College, Mumbai, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>50 years old male presented with c/o Hiccups (Daily, Nocturnal, Not responding to PPI, Baclofen), Dysphagia(Solid, Liquid), Weight loss (4-5 kgs), Heartburn since 2 years without any previous comorbidities. Upper GI scopy showed LA Grade C esophagitis without any food residue with Narrowing at GEJ(Ekcardt score–2) Impression was kept as ?Peptic esophageal stricture. Stricture was dilated with SG dilator(14 mm) and PPI was given for 1 month. 6 months later, No improvement in symptoms were noted with persistent hiccups and heartburn with progression of dysphagia from occasional to with every meal with Weight loss of additional 5 kgs. Repeat endoscopy showed normal esophagus with passage of scope across GEJ with moderate difficulty(Ekcardt score–5).High resolution manometry was performed which showed Mean IRP of 10 wet swallows -7.9 mm Hg,LES pressure – 14.1 mm Hg,40 % swallows showed premature contraction(DL <4.5 sec). Barium swallow showed luminal narrowing at GEJ with hold up of barium column in lower half of esophagus s/o outflow obstruction. Diagnosis was made as Distal esophageal spasm. Patient was managed with POEM with long myotomy (8 cm).2 weeks post dilatation, patient was symptom free, hiccups disappeared(Ekcardt score-0).HRM(Post 2 weeks) showed Mean IRP of 10 wet swallows -11.27mm Hg, LES pressure – 40.1 mm Hg, normal distal latency > 4.5 sec with Normal study. It was a First case of distal esophageal spasm presenting as hiccups. It requires high index of suspicion for diagnosis. HRM and TBE should be attempted in intractable hiccups</p><p><b>PP-03-119</b></p><p><b>Understanding Barrett's Esophagus in Systemic Sclerosis: Insight into Phatogenesis and Clinical Management</b></p><p><b>Masyfuk Zuhdi Jamhur</b><sup>1</sup>, Fhathia Avisha<sup>2</sup>, Crisdina Suseno<sup>3</sup> and Rizki Trismimanda<sup>4</sup></p><p><sup>1</sup><i>General Practitioner, Medical Faculty Of Andalas University, Padang, Indonesia;</i> <sup>2</sup><i>Departement of Internal Medicine, Ciptomangunkusumo Hospital, Jakarta, Indonesia;</i> <sup>3</sup><i>General Practitioner, Mentawai Island District Hospital, Mentawai Regency Island, Indonesia;</i> <sup>4</sup><i>General Practitioner, Kudungga District Hospital, East Kutai, Indonesia</i></p><p><b><i>Introduction:</i></b> Gastrointestinal involvement in systemic sclerosis is heterogeneous, ranging from asymptomatic to significant dysmotility. Severity of GI involvement has shown to be a predictor of worse prognosis and mortality in patients with systemic sclerosis. An increased risk of Barrett esophagus in systemic sclerosis due to chronic reflux. We report a case of Barrett’s Esophagus in patients with systemic sclerosis.</p><p><b><i>Case Description:</i></b> A 35-year-old female with a long history of diffuse cutaneous systemic sclerosis (mRSS 27) treated with mycophenolate sodium. The patient comes with chief complaints of heartburn, regurgitation, chest pain, and dysphagia for one year. The esophagogastroduodenoscopy examination showed esophagitis, pangastritis, and hiatal hernia. An abdominal ultrasound examination showed complex ascites. Biopsies confirmed Barrett’s esophagus. The patient was treated with lifestyle modification, PPI, Pro-motility agents, and mucoprotective. The Patient showed clinical improvement in GERD symptoms</p><p><b><i>Discussion:</i></b> Barrett's esophagus is a transformative process wherein acid exposure causes the endogenous stratified squamous epithelium in the lower region of the esophagus to change into a metaplastic columnar epithelium. In systemic sclerosis, esophageal involvement can result from vasculopathy and tissue hypoxia, leading to dysphagia and GERD. Persistent acid reflux increases the risk of Barrett’s esophagus which can progress to adenocarcinoma. Patients with Barrett’s esophagus should be treated with PPI. The management is mainly aimed at alleviating the symptoms and preventing complications. Early detection is very important to prevent malignancy. Routine screening for Barrett’s esophagus is essential because of the high risk of neoplastic progression.</p><p><b><i>Keywords:</i></b> Barrett's Esophagus, Systemic Sclerosis, Gastroesophageal Reflux Disease</p><p><b>PP-03-120</b></p><p><b>Esophageal Gastrointestinal Stromal Tumor: Incidental Finding During Urologic Workup of a Filipino Patient</b></p><p><b>Dovenne Jade Cania</b> and Jenny Limquiaco</p><p><i>Cebu Doctors' University Hospital, Cebu City, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. It most commonly arises from the stomach and less commonly from the small intestine and colorectum. GIST which arises from the esophagus is a very rare occurrence and is only seen in <1% of all GISTs. Most cases are asymptomatic and is discovered incidentally for workup of another entity.</p><p><b><i>Clinical Presentation:</i></b> Index case is a 65 year-old male businessman from Cebu who presented with an incidental finding of submucosal distal esophageal mass on CT-scan during work-up for a urologic pathology. Patient has no known comorbidities other than benign prostatic hypertrophy. He has no gastrointestinal complaints and has largely unremarkable physical examination. Contrast abdominal and chest CT-scan confirmed and delineated the distal esophageal mass. Patient underwent abdominal laparoscopic excision with Nissen fundoplication. Post-operative course was unremarkable as well and patient was discharge in stable condition. Final histopathology of the esophageal mass was consistent with gastrointestinal stromal tumor with a positive immunostaining for CD117. Patient was then referred to oncology service and is currently undergoing imatinib therapy.</p><p><b><i>Discussion:</i></b> Esophageal GIST is a rare disease entity and is usually asymptomatic. As in all subepithelial tumors, prompt removal of tumor is part of definitive management. While large evidences are still lacking, it has been recommended that imatinib be given for all extra-gastric GISTs.</p><p><b>PP-03-121</b></p><p><b>Prognostic Factors Of Mortality In Biliary Tract Cancer : A Systematic Review</b></p><p><b>Fernando Dharmaraja</b><sup>1</sup>, Felix Wijovi<sup>1</sup>, Angeline Tancherla<sup>1</sup>, Ignatius Bima Prasetyo<sup>2</sup> and Andree Kurniawan<sup>2</sup></p><p><sup>1</sup><i>Univesitas Pelita Harapan, Tangerang, Indonesia;</i> <sup>2</sup><i>Internal Medicine Department Siloam Hospitals lippo Vilage, Tangerang, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Biliary tract cancer is a rare gastrointestinal cancer. Despite known prognostic factors, the mortality rate remains high. This paper reviews and evaluates prognostic factors affecting mortality rates in biliary tract cancer patients.</p><p><b><i>Materials and Methods:</i></b> Studies from PubMed, PMC, and ScienceDirect were collected using keywords “Biliary Tract cancer,” “Prognostic Factor,” and “Mortality rate.” Only English full-text studies published in the past five years were included. Data on prognostic factors and overall survival (OS) were analyzed. The risk of bias was assessed using the Quality in Prognosis Studies (QUIPS) tool.</p><p><b><i>Results:</i></b> Twenty-five studies involving 8,414 patients were reviewed. Significant prognostic factors for OS were identified: demographic factors (old age, male sex), nutritional factors (low pre-operative cachexia index, low prognostic nutritional index, radiological sarcopenia), and laboratory factors (elevated serum lactate dehydrogenase, neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, platelet-to-lymphocyte ratio, CEA > 10 μg/L, CA19-9 > 39 IU/ml, reduced albumin levels, elevated CRP). Types of treatment (RFA, radiotherapy, lymphadenectomy, FFP infusion) and tumor characteristics (poor differentiation, gallbladder tumor, diameter > 5 cm, multiple tumors, ALB ≥ 35 g/L, lymph node metastasis, lymphovascular invasion, proximal ductal margin) were also identified. Tumor expression (β-catenin, DKK1+, Tropomyosin receptor kinase+, pyruvate kinase M2 upregulation, CD3+, CD8+, Foxp3+, HER2+, Leucine-Rich α-2-Glycoprotein-1 upregulation, low ribosomal receptor for activated C-kinase 1 expression), genetic factors (CDKN2B-AS1 overexpression, Transforming growth factor β CircLTBP2), and comorbidities (infectious complications) were significant as well.</p><p><b><i>Conclusion:</i></b> This review highlights common and newly identified prognostic factors, expected to guide patient-centered clinical management to reduce biliary tract cancer mortality.</p><p><b>PP-03-122</b></p><p><b>Hematological Parameters Comparison In Colorectal Cancer, Non Colorectal Cancer Patients: Focus in NLR, PLR, LMR</b></p><p><b>Ety Febrianti</b></p><p><i>Sriwijaya University, Palembang, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Introduction:</i></b> Colorectal cancer (CRC) is a gastrointestinal malignancy with high morbidity and mortality rates in Indonesia. Histopatology remains the gold standard for CRC detection and screening. Chronic inflammation and hosts immune responses are known to play important roles in tumorigenesis and cancer progression. This inflammation affects the results of hematological examination. Therefore, parameters such as Neutrophil-Lymphocyte Ratio (NLR), Platelet-Lymphocyte Ratio (PLR), and Lymphocyte-Monocyte Ratio (LMR) are expected to provide information on tumor cell development.</p><p><b><i>Objective:</i></b> This study aims to evaluate the role of NLR, PLR, and LMR in distinguishing CRC and non-CRC at hospital Dr. Mohammad Hoesin Palembang.</p><p><b><i>Methods:</i></b> The study was conducted using a cross-sectional design with a total of 141 suspected CRC patients, with 71 CRC patients and 70 non-CRC patients undergoing colonoscopy and histopathology examinations from April to August 2024.</p><p><b><i>Results:</i></b> There was a comparison of the mean NLR ratio in the CRC group (4.6 ± 6.4) and non-CRC (3.9 ± 4.07). Comparison of the mean PLR ratio in the CRC group (19.3 ± 16.05) and non-CRC (20.8 ± 29.36). Comparison of the mean LMR ratio in the CRC group (3.18 ± 1.85) and non-CRC (3.3 ± 1.8).</p><p><b><i>Conclusion:</i></b> An increased NLR ratio and a decreased PLR and LMR ratio can be potential parameters in differentiating CRC and non-CRC patients.</p><p><b><i>Keywords:</i></b> NLR, PLR, LMR, CRC, Non-CRC</p><p><b>PP-03-123</b></p><p><b>Clinicopathological Profile of Gastric Cancer Patients at Cipto Mangunkusumo National Hospital: A Five-Year Review (2018-2023)</b></p><p><b>Yovita Gotama</b><sup>1</sup>, Steven Alvianto<sup>1</sup> and Rabbinu Rangga Pribadi<sup>2</sup></p><p><sup>1</sup><i>School Of Medicine And Health Sciences, Atma Jaya Catholic University Of Indonesia, Jakarta, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The study highlights gastric cancer's critical demographic and clinical characteristics at Cipto Mangunkusumo National General Hospital between January 2018 and December 2023.</p><p><b><i>Materials and Methods:</i></b> A retrospective study was conducted using medical records of patients diagnosed with gastric cancer at Cipto Mangunkusumo National General Hospital between January 2018 and December 2023. Data collected included demographic information (gender, age, ethnicity, religion), clinical presentation (symptoms), type of gastric cancer, location, metastatic, and treatment modalities. Statistical analysis was performed to identify trends.</p><p><b><i>Results:</i></b> A total of 300 patients were diagnosed with gastric cancer during the study period. The patients were divided into 60 patients with early onset (≤45 years) and 240 patients with conventional (>45 years). There was a significantly higher proportion of the conventional group comorbid hypertension compared with the early-onset group (0 and 13.75%; p=0.000) and also the symptoms of abdominal pain (35.4% and 13.3%, p=0.001). Otherwise, in the early-onset group, significantly higher abdominal distention compared to the conventional group (13.3% and 5%, p=0.037). The most common tumour location for both groups is non-cardia and the histological type from WHO classification is intestinal type. The majority of metastasis is liver (18.3%), lung (5.7%), and peritoneum (2.3%). The modality treatment for resectable cancer through surgery is also 46.3% of the total patients.</p><p><b>PP-03-124</b></p><p><b>Comprehensive meta-analysis of cd44 as diagnostic marker, prognostic and targeting therapy for colorectal cancer</b></p><p><b>Burhan Gunawan</b> and Raymond Sebastian Purwanta</p><p><i>Sumber Waras Hospital, West Jakarta, Jakarta Barat, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> Activation number of signalling pathway by CD44 linked to tumour growth, migration, chemoresistance. But its role in CRC remains unclear. Aim of this study was to investigate CD44 expression, relationship to prognosis, and quantify clinical effect various drug targeting CD44 in CRC.</p><p><b><i>Method:</i></b> The expression level of CD44 in colorectal cancer was analysed in TCGA, GEPIA2, HPA, and GEO database. The Kaplan-Meier Plotter database was used to analyse the relationship between CD44 expression and prognostic value (OS, PFS, PPS). The clinical study used CD44 targeting drug for colorectal cancer included in network meta-analysis using Revman Cochrane Library, following PRISMA guideline.</p><p><b><i>Result:</i></b> The median expression value of CD44 in COAD tumour sample was 151.88 and in normal sample was 49.38, and CNV CD44 found in 78/453 (17.21%) subject. The CD44 significantly highly expressed in colon cancer sample compare to normal adjacent tissue sample (p=0.008). Immunohistochemically stained tissue sampel showed that CD44 significantly overexpressed in tumor tissue. The PFS of CRC patient with high CD44 expression were significantly poor (p=0.0098). Total 10 RCT Phase 1-3 Studies, use CD44 Targeting Drug or Antibody Monoclonal, with total 2627 subjects, included in meta-analysis. The PFS mean difference was 2.40 (95% C.I. 1.15-3.65) month, p=0.0002, I² = 100%.</p><p><b><i>Conclusion:</i></b> CD44 is highly expressed in CRC which can be used as diagnostic and prognostic marker. This is also the first meta-analysis study about CD44 as targeting therapy in human CRC. The study shows the potential drug to regulating CD44 expression in the future for CRC.</p><p><b>PP-03-125</b></p><p><b>Berberine exhibits the capacity to impede the progression of gastric cancer by modulating oxidative stress</b></p><p><b>Xiaodong Han</b> and Xin Chen</p><p><i>Tianjin Medical University General Hospital, Tianjin, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Objective:</i></b> The goal of this study is to elucidate the mechanisms underlying the anti-gastric cancer effects of berberine using network pharmacology and subsequently validate these findings through in vitro experiments.</p><p><b><i>Materials and Methods:</i></b> Target information for the effects of berberine and gastric cancer were collected from databases, and the intersection of these targets were used to construct a \"drug-disease-target\" network as well as a protein-protein interaction (PPI) network. The shared targets underwent GO functional and KEGG pathway enrichment analyses. In vitro experiments were conducted to investigate the impact of berberine on the activity, migration ability, and intracellular ROS levels of AGS and MKN45 gastric cancer cells. Western blot analysis was utilized to examine changes in the Nrf2/HO-1, HIF-1α, and EMT pathways.</p><p><b><i>Results:</i></b> The network pharmacology analysis identified 224 common targets for berberine and gastric cancer. The GO analysis revealed that the top biological processes involving these targets included responses to oxidative stress, oxygen levels, oxidative stress in cells, reactive oxygen species, and hypoxia. The KEGG analysis indicated that pathways such as PI3K-AKT and HIF-1 may be key signaling pathways for the anti-gastric cancer effects of berberine. The in vitro experimental results demonstrated that berberine significantly suppressed the activity and migration ability of gastric cancer cells, elevated intracellular ROS levels, and inhibited the expression of proteins involved in the Nrf2/HO-1, HIF-1α, and EMT pathways in the cells.</p><p><b><i>Conclusion:</i></b> Modulating oxidative stress may represent a crucial mechanism through which berberine inhibits the progression of gastric cancer.</p><p><b>PP-03-126</b></p><p><b>Incidence and Mortality of Cancer of Rectum and Anus in Chinese: a trend analysis</b></p><p><b>Junjie Huang</b>, Shui Hang Chow, Chenwen Zhong, Sze Chai Chan and Martin Wong</p><p><i>The Chinese University Of Hong Kong, Sha Tin, Hong Kong</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of rectum and anus cancer.</p><p><b><i>Methods:</i></b> Data on new cases and deaths related to rectum and anus cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).</p><p><b><i>Results:</i></b> In 2020, a total of 1,898 new cases of rectum and anus cancer were reported in Hong Kong, with an ASR of 11.6. The incidence was significantly higher among males (ASR=15.1) compared to females (ASR=8.4), and in the older population aged over 50 years (ASR=49.1) compared to the younger population (ASR=2.2). There were 745 newly reported rectum and anus cancer-related deaths in Hong Kong, with an ASR of 4.3. The mortality was also notably higher in males (ASR=5.2) than in females (ASR=3.1), and in the older population (ASR=17.4) compared to the younger population (ASR=0.3). Trend analysis revealed a significant decreasing incidence of rectum and anus cancer in the older age group (AAPC: -1.6, 95% CI: -3.1, -0.01, P=0.05) and among females (AAPC: -1.8, 95% CI: -3.1, -0.4, P=0.02).</p><p><b><i>Conclusion:</i></b> This population-based study demonstrated a decreasing trend in the incidence of rectum and anus cancer, particularly among the older population and females in Hong Kong. However, the overall disease burden remains significant, highlighting the need for further reducing the burden of rectum and anus cancer in the region.</p><p><b>PP-03-127</b></p><p><b>Incidence, Mortality and Trends of Oesophageal Cancer in Chinese: a population-based study</b></p><p><b>Junjie Huang</b>, Ching Fung Yat, Chenwen Zhong, Sze Chai Chan and Martin Wong</p><p><i>The Chinese University of Hong Kong, Sha Tin, Hong Kong</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> This study aimed to provide a comprehensive analysis of the oesophageal cancer burden, including the incidence and mortality trends by sex and age group.</p><p><b><i>Methodology:</i></b> Data on new oesophageal cancer cases and deaths were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated using the Segi-Doll world population as the standard. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC) in the incidence and mortality of oesophageal cancer, stratified by sex and age group.</p><p><b><i>Results:</i></b> In 2020, there were 399 newly reported oesophageal cancer cases (ASR=2.3) and 318 oesophageal cancer-related deaths (ASR=1.8) in Hong Kong. The incidence and mortality of oesophageal cancer were higher among males compared to females (incidence ASR: 4.6 vs. 0.7; mortality ASR: 3.2 vs. 0.5) and in the older population aged 50 years and above (incidence ASR: 10.6 vs. 0.2; mortality ASR: 8.14 vs. 0.18). Both the incidence and mortality trends of oesophageal cancer showed decreasing patterns over time, with a notable decline observed in the older population for both incidence (AAPC: -3.65, 95% CI: -5.01, -2.27, p<0.001) and mortality (AAPC: -3.93, 95% CI: -4.98, -2.87, p<0.001).</p><p><b><i>Conclusion:</i></b> This study revealed an overall decreasing trend in the incidence and mortality of oesophageal cancer in Hong Kong, particularly among the older population. Despite this favorable trend, continued monitoring and the promotion of healthy lifestyles are essential to maintain the decreasing pattern of oesophageal cancer burden in the Hong Kong population.</p><p><b>PP-03-128</b></p><p><b>Proton pump inhibitors and gastric cancer: A large-scale propensity score-matched analysis using the UK Biobank</b></p><p><b>Cheal Wung Huh</b><sup>1</sup>, Da Hyun Jung<sup>2</sup> and Hae-Ryong Yun<sup>1</sup></p><p><sup>1</sup><i>Yongin Severance Hospital, Yonsei University College Of Medicine, Seoul, South Korea;</i> <sup>2</sup><i>Severance Hospital, Yonsei University College Of Medicine, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> We aimed to elucidate whether new proton pump inhibitor (PPI) users are at increased risk of gastric cancer compared with non-PPI users or new histamine-2 receptor antagonist (H2RA) users.</p><p><b><i>Design:</i></b> A population-based cohort study was conducted using data from the UK Biobank from 1990–2016. We compared the incidence rates of gastric cancer after a minimum of 1-year drug exposure between PPI users and non-PPI or H2RA users using Cox proportional hazards models. Secondary analyses assessed duration and dose–response associations. Sensitivity analyses, incorporating various matching ratios, extended lag periods, and propensity score stratification, were performed.</p><p><b><i>Results:</i></b> After large-scale propensity score matching, we included 42,732 new PPI, 42,732 non-PPI, and 4,762 new H2RA users. During a median follow-up of 6.7 years, PPI users had a 2.32-fold higher risk of gastric cancer than non-PPI users (HR 2.324, 95% CI 1.646–3.282), with numbers needed to harm of 488 and 442 at 2 and 4 years, respectively. Compared with H2RA users, PPI users also showed a significantly higher incidence of gastric cancer (HR 5.343, 95% CI 1.557–18.335). The risk of gastric cancer tended to increase with prolonged cumulative duration and higher cumulative doses of PPI use. Moreover, these results remained robust after adjustments for various lag periods, matching ratios, and propensity score stratification.</p><p><b><i>Conclusion:</i></b> PPI use was associated with a higher incidence of gastric cancer, albeit with a low absolute risk. Therefore, long-term PPI use should be approached cautiously, even in regions with a low risk of gastric cancer.</p><p><b>PP-03-129</b></p><p><b>FOLFIRINOX Versus Gemcitabine/Nab-paclitaxel in Metastatic Pancreatic Cancer: A Real-World Data Analysis Including NGS Panel Insights</b></p><p>Jeehoon Kim, Galam Leem, Hee Seung Lee, Moon Jae Chung, Jeong Youp Park, Seungmin Bang, Seung Woo Park, Si Young Song and <b>Jung Hyun Jo</b></p><p><i>Yonsei University College of Medicine, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> FOLFIRINOX and Gemcitabine/Nab-paclitaxel are widely used 1st-line regimens in metastatic pancreatic cancer. We would like to present real-world data of 12 years of experience with both regimens including genomics insights.</p><p><b><i>Aims & Methods:</i></b> Patients with metastatic disease were retrospectively included from January 2010 to December 2022. Outcomes were overall survival (OS) and progression free survival (PFS). Median survival time was calculated using Kaplan-Meier estimates adjusted with 1:1 Propensity Score Matching (PSM). Tissue Next Generation Sequencing (NGS) panel data was analyzed.</p><p><b><i>Results:</i></b> A total of 521 patients, 251 patients received FOLFIRINOX and 270 patients received Gemcitabine/Nab-paclitaxel as a first-line regimen. FOLFIRINOX group were younger (61.8 vs. 65.9, p<0.001). Before PSM, FOLFIRINOX group showed prolonged median OS (11.1 vs 7.8 months, p=0.002) and PFS (8.8 vs. 5.8 months, p=0.002) compared to Gemcitabine/Nab-paclitaxel group. After PSM, FOLFIRINOX group still showed prolonged median OS (11.1 vs 9.0 months, p=0.010) and PFS (9.2 vs 6.4 months, p=0.001) than Gemcitabine/Nab-paclitaxel group. NGS was available from 31 and 22 patients in the FOFLIRINOX and Gemcitabine/Abraxane groups, respectively. The most frequently mutated genes were KRAS (94.3%), TP53 (84.9%), CDKN2A (24.5%), SMAD4 (24.5%), ARID1A(13.2%) and RNF43(9.4%). On Cox Regression Analysis, RNF43 mutation was correlated with poor PFS with Gemcitabine/Nab-paclitaxel (HR, 4.849; 95% CI, 1.150-20.450, p=0.032).</p><p><b><i>Conclusion:</i></b> FOLFIRINOX showed prolonged OS and PFS compared to Gemcitabine/Nab-paclitaxel. The NGS data suggest RNF43 could be a potential predictive marker for the response to Gemcitabine/Nab-Paclitaxel, however, a larger size confirmation study is needed to validate.</p><p><b>PP-03-130</b></p><p><b>Predictive factors for malignant lesions among colonoscopy cohorts in a tertiary hospital in indonesia</b></p><p><b>Alvin Johan</b><sup>1</sup> and Saskia Aziza Nursyirwan<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;</i> <sup>2</sup><i>Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To determine factors that may predict malignant lesions among patients who underwent colonoscopy in Indonesia</p><p><b><i>Materials and methods:</i></b> This retrospective cohort study used colonoscopy registry data at Dr. Cipto Mangunkusumo General Hospital during the 2018-2022 period. Binomial logistic regression was performed to ascertain the effects of age, sex, education, job status, smoking history, unhealthy dietary habits, and gastrointestinal symptoms on the likelihood that participants have a malignant lesion on colonoscopy.</p><p><b><i>Results:</i></b> Lesions suggestive of malignancy were found in 301 of 1,694 colonoscopy reports (17.7%).</p><p>Linearity of the continuous variables concerning the logit of the dependent variable was assessed, and a Bonferroni correction was applied using all 11 terms in the model resulting in statistical significance being accepted when p < .0045. Age as the continuous independent variable was linearly related to the logit of the dependent variable. There were no residuals. The logistic regression model was statistically significant, χ²(10) = 129.171, p < .001. The model explained 12.1% (Nagelkerke R2) of the variance in colorectal malignancy and correctly classified 82.5% of cases.</p><p>The positive predictive value was 57.69% and the negative predictive value was 82.9%. Of the nine predictor variables, six were statistically significant: older age, joblessness, gastrointestinal bleeding, and weight loss were associated with higher odds of malignant lesions. Female sex and higher education were associated with lower odds of malignant lesions. (as shown in the table)</p><p><b><i>Conclusion:</i></b> Age, sex, job status, education level, symptoms of gastrointestinal bleeding, and weight loss were predictive of malignant lesions on colonoscopy.</p><p><b>PP-03-131</b></p><p><b>Long-term outcomes of gastric mucosa-associated lymphoid tissue lymphoma A multi-center retrospective study in Honam, Korea</b></p><p><b>Jin Won Kim</b><sup>1</sup>, Seon-Young Park<sup>1</sup>, Ga-Ram You<sup>2</sup>, Wan-Sik Lee<sup>2</sup>, Byung-Chul Jin<sup>3</sup>, Seung-Young Seo<sup>3</sup>, Jung-In Lee<sup>4</sup>, Young-Dae Kim<sup>4</sup>, Chan-Guk Park<sup>4</sup>, Dong-Han Yeom<sup>5</sup> and Suck-Chei Choi<sup>5</sup></p><p><sup>1</sup><i>Chonnnam National University Hospital, 42, Jebong-ro, Dong-gu, Gwangju, South Korea;</i> <sup>2</sup><i>Chonnam National University Hwasun Hospital, 322, Seoyang-ro, Hwasun-eup, Hwasun-gun, South Korea;</i> <sup>3</sup><i>Jeonbuk National University Hospital, Jeonju-si, South Korea;</i> <sup>4</sup><i>Chosun University Hospital, Gwangju, South Korea;</i> <sup>5</sup><i>Wonkwang University Hospital, Iksan-si, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> MALT lymphoma constitutes 50% of all lymphomas in the stomach and is linked to Helicobacter pylori (H. pylori). This Study explores treatment effectiveness, remission, and relapse factors in gastric MALT lymphoma patients under various treatments</p><p><b><i>Methods:</i></b> A retrospective review was performed of 398 patients with gastric MALT lymphoma from 5 hospitals in Honam Province over a 15-year period with a minimum follow-up of 48 weeks.</p><p><b><i>Results:</i></b> Mean age at diagnosis was 55.5 years with 176 males. H. pylori infection was in 293 (73.6%) patients, isolated lesion in 229 (57.5%) and stage IE in 363 (91.2%). There were no significant differences in age, sex, and stage at the diagnosis between H. pylori-positive group (HPP) and H. pylori-negative group (HPN). In the HPP group, 290 patients received eradication, 74 radiotherapy and 18 chemotherapy. In the HPN group, 77 received eradication, 56 radiotherapy and 19 chemotherapy. Overall complete remission rate was 97.0% [98.3% in HPP and 93.3% in HPN (P=0.018)]. HPP had higher complete remission rate compared to HPN (aOR 4.1). 32(8.3%) patients relapsed [23(7.9%) in HPP and 10(10.2%) in HPN]. Non-IE Modified Ann Arbor Stage (HR 2.4), presence of LAP (HR 1.9) and lesions in multiple locations (HR 2.1) were correlated with higher cumulative incidence of relapse (CIR). The presence of LAP was correlated with higher CIR in multivariate Cox regression analysis (aHR 2.9).</p><p><b><i>Conclusions:</i></b> H. pylori infection status is associated with complete remission rates and presence of LAP is associated with the relapse.</p><p><b>PP-03-133</b></p><p><b>Burden of young-onset colorectal cancer: The India perspective from Global Burden of Disease study 2021</b></p><p><b>Laalithya Konduru</b><sup>1,4</sup>, Simranjeet Singh Dahia<sup>1,2</sup> and Arsh Garg<sup>3</sup></p><p><sup>1</sup><i>Flinders University, Adelaide, Australia;</i> <sup>2</sup><i>The University of Adelaide, Adelaide, Australia;</i> <sup>3</sup><i>Government Medical College, Patiala, India;</i> <sup>4</sup><i>Sri Jagannath Health Care and Research Center, Dhanbad, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colorectal cancer (CRC) is the third highest cause of death worldwide. The Global Burden of Disease (GBD) data reveals a rising trend in young-onset CRC (YOCRC; CRC presenting in patients aged 15-49 years) globally. However, the burden of YOCRC in India remains unknown. We aimed to investigate the burden of YOCRC in India using data from GBD 2021.</p><p><b><i>Materials and Methods:</i></b> The GBD India Compare tool (1990-2021) was queried for “colon and rectal cancer” as the “cause” and “incidence,” “deaths,” and “DALYs” as the “measure” for the 15-49 years age group. The national and state-wise burden of YOCRC in India was determined, and the Human Development Index (HDI) was used to evaluate associations between human development and YOCRC burden. The YOCRC burden attributable to leading dietary, behavioral, and metabolic risk factors was also estimated.</p><p><b><i>Results:</i></b> National YOCRC incidence, mortality, and DALY rates increased significantly, particularly among males, between 1990 and 2021. In 2021, YOCRC incidence was highest in Uttarakhand, Uttar Pradesh, and Kerala, with Uttar Pradesh showing the highest YOCRC burden among females nationwide. HDI was not associated with YOCRC burden. This contrasts with global trends, which showed that YOCRC burden increases with increasing HDI. Diets low in milk, whole grains, and calcium remained the most important risk factors of YOCRC from 1990 to 2021.</p><p><b><i>Conclusions:</i></b> India's YOCRC burden is increasing, regardless of HDI, driven by dietary, behavioral, and metabolic risk factors. The findings highlight the need for targeted interventions and greater awareness to address this public health challenge.</p><p><b>PP-03-134</b></p><p><b>Deciphering Changes in Immune Cells Associated with H. pylori-Induced Gastric Carcinogenesis via Single-Cell RNA Transcriptomics</b></p><p><b>Yonghwan Kwon</b></p><p><i>School Of Medicine/kyungpook National University, Daegu, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Backgrounds:</i></b> Tumor microenvironment (TME) complex cellular components and heterogeneity have not been fully resolved in gastric carcinogenesis. Helicobacter pylori (H. pylori) is known to be the major cause of gastric carcinogenesis, the relation between H. pylori and TME were not fully elucidated.</p><p><b><i>Methods:</i></b> we performed single-cell profiling of H, pylori related normal gastric mucosa, gastric dysplasia (GD), and early gastric cancer (EGC), especially intestinal type, identifying alterations in TME cell states and compositions.</p><p><b><i>Results:</i></b> Transcriptome profiles of 70483 cells in the H. pylori infected 7 normal, 5 gastric adenoma, and 4 EGAC tissue samples were analyzed from 12 patients. After dimension reduction and clustering, 10 clusters of cells were identified. In the analysis of macrophages decoded cellular divergence in premalignant and malignant tumors, we identified 10 key genes were overexpressed in EGC compared with normal and GD and SPP1+/INHBA+ macrophages were significantly overexpressed in EGC is a central coordinator of H. pylori related gastric carcinogenesis (Fig 1). the proportion of exhausted CD8+ T cells and regulatory T cells increased. The ratio of cytotoxic CD8+ T cells decreased as GD progressed to EGC.</p><p><b>PP-03-135</b></p><p><b>Malignancy Predicting Model of Pancreatic Cystic Neoplasm in Contrast-Enhanced CT by Machine-Learning and Radiomics</b></p><p><b>Hong Sik Lee</b> and Jae Min Lee and Jong Jin Hyun and Jeong Whan Choi and Kang Won Lee</p><p><i>Korea University Anam Hopsital, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Accurate identification of PCNs is crucial due to their diverse characteristics. To improve diagnostic precision, this study employs radiomics and machine learning to develop a predictive model for PCN malignancy risk, utilizing CT imaging data.</p><p><b><i>Materials and Methods:</i></b> This retrospective study included 71 pts who underwent op for PCN and had CT scans within 90 days before op. PCNs were categorized as benign or malignant based on pathological results. We conducted texture analysis on segmented cystic fluid using an image processing program, calculating the standard deviation of gray-scale histograms(SDGSH) within the segmented area to detect increased heterogeneity. From the volume of interest in PCNs, 824 radiomics features were extracted. Radiomics and clinical features were selected using a random forest regressor, and the PCN classification model was trained and validated using a random forest classifier with 20-fold stratified cross-validation.</p><p><b><i>Results:</i></b> The SDGSH representing texture heterogeneity, increased progressively from the non-dysplasia group to the invasive carcinoma group, with significant differences noted. The SDGSH demonstrated good predictive power for malignancy in pre-op CT images, with an AUC of 0.81 (95% CI 0.67-0.95). An optimal cut-off value of 17.597 yielded 87.5% sensitivity and 69.6% specificity. In internal validation, the radiomics-based machine learning model showed a meaningful AUC of 0.83 (95% CI 0.72-0.94), with a sensitivity of 81.25% and a specificity of 64.10%.</p><p><b><i>Conclusions:</i></b> Radiomics technology, specifically SDGSH correlating with textural heterogeneity, and machine learning models analyzing cyst texture heterogeneity in CT images, were found to be good predictors of malignancy risk in PCN.</p><p><b>PP-03-136</b></p><p><b>Association between nlr, mlr, plr, la, and cea levels in colorectal cancer patients with metastasis</b></p><p><b>Muhammad Nur Ikhsan Liwang</b><sup>1,2</sup>, Rini Rahmawarni Bachtiar<sup>1,2</sup>, Muhammad Luthfi Parewangi<sup>1,2</sup>, Nu’man AS Daud<sup>1,2</sup>, Fardah Akil<sup>1,2</sup>, Susanto H. Kusuma<sup>1,2</sup> and Amelia Rifai<sup>1,2</sup></p><p><sup>1</sup><i>Division of Gastroentyerology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;</i> <sup>2</sup><i>Centre of Gastroenterology-Hepatology HAM Akil, DR.Wahidin Sudirohusodo General Hospital, Makassar, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract</b></p><p><b><i>Objectives:</i></b> Colorectal cancer (CRC) is the third most common cancer worldwide. Early-stage diagnosis with enhanced screening methods is vital as metastases and recurrences increase mortality. The study aims to determine the predictive value of NLR, MLR, PLR, LA, and CEA levels as the metastases predictor of colorectal cancer (CRC).</p><p><b><i>Materials and methods:</i></b> A case-control study was conducted using secondary data (patient medical records) of colorectal cancer patients, CEA and CBC data at Dr Wahidin Sudirohusodo Hospital Makassar between January 2023 and June 2024. The data were grouped and then statistically analyzed using SPSS version 29.0 for Windows.</p><p><b><i>Results:</i></b> The number of patients consisted of 34 males (55.7%) and 27 females (44.3%), the most age >60 years (49.2%), the most tumour location in the rectum (54.1%), with mean ± SD NLR: 3.06 ± 0.56, MLR: 2.26 ± 0.58, PLR 258.92 ± 98.7, LA 4574 ± 1230, and CEA level 49.98 ± 69.18. NLR cut-off value 2.56, PLR 258, MLR 2.38, LA 4413, and CEA 10 ng/mL. NLR, PLR and CEA values higher than the cut-off had a higher incidence of metastasis (p<0.05), while lower LA had a higher metastasis rate (p<0.05).</p><p><b><i>Conclusion:</i></b> There is an association between NLR, PLR, CEA, and LA in the presence of metastasis in colorectal cancer.</p><p><b>PP-03-137</b></p><p><b>Cold Snare Polypectomy for Pouch Polyps After Colectomy in Familial Adenomatous Polyposis: A Case Report</b></p><p><b>Tatsuya Matsumoto</b></p><p><i>Kitakyushu Municipal Medical Center, Fukuoka, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Familial Adenomatous Polyposis (FAP) is an autosomal dominant disorder caused by APC gene mutations, characterized by multiple colorectal adenomas. Main causes of death in FAP patients include colorectal cancer, desmoid tumors, and upper gastrointestinal cancers. Even after total colectomy, adenoma and cancer risks persist in residual rectal mucosa and ileal pouch. This report presents a case where Cold Snare Polypectomy (CSP) effectively treated multiple ileal pouch polyps in an FAP patient post-subtotal colectomy.</p><p><b><i>Case:</i></b> A 62-year-old male with FAP underwent total colectomy, subtotal rectal resection, and ileal J-pouch anal anastomosis at 34. For 10 years post-surgery, endoscopy revealed only solitary rectal polyps. At 53, multiple polyps were detected in residual rectal mucosa. Large polyps were removed by Endoscopic Submucosal Dissection (ESD), but numerous polyps subsequently appeared in residual rectal mucosa and ileal pouch.</p><p>CSP was performed every 6-12 months for newly developed polyps. No rectal or small intestinal cancer has been detected to date. At 61, the patient underwent endoscopic papillectomy for duodenal ampullary adenoma, with no high-risk tumors or cancer developing in the duodenum.</p><p><b><i>Discussion:</i></b> CSP was the primary method for controlling multiple ileal pouch polyps, proving safe and minimally invasive. This approach may potentially avoid surgical intervention. However, further research is needed to establish long-term effectiveness and prognosis of CSP in such cases. This case suggests CSP may effectively control residual gastrointestinal tumors in FAP patients post-total colectomy, offering a promising alternative to more invasive treatments.</p><p><b>PP-03-138</b></p><p><b>Bioelectrical Impedance Analysis Study of Colorectal Cancer Patients in Indonesian Tertiary Hospital</b></p><p><b>Dewi Mustikarani</b></p><p><i>Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Various studies have linked the severity of cancer and a decrease in body composition. The use of Bio Impedance Analysis (BIA) in determining body composition analysis has been considered the better and more feasible choice in hospital settings. This study aims to find the association between BMI, fat mass (FM), fat mass index (FMI), free fat mass (FFM), free fat mass index (FFMI), cachexia, and cancer staging in Indonesian tertiary hospital.</p><p><b><i>Materials and methods:</i></b> This is a cross-sectional study. The analysis was performed using BIA from Seca. The baseline data was taken during the patient's last visit in 2021-2024. No significance was found among BIA results, cachexia, and cancer staging. The data were analyzed using SPSS version 25.</p><p><b><i>Result:</i></b> There are 22 colorectal patients in this study. The mean age is 52 years, and 11 patients are male. With CI 95%, there are significant associations between age and cancer stage (p=0.006), BMI and fat mass (p<0.001), BMI and FFM (p=0.004), and BMI and FFMI (p<0.001). There is no significance found among BMI, cachexia, and cancer staging.</p><p><b><i>Conclusion:</i></b> The progressivity of the cancer and its treatment can cause metabolic changes that affect body composition. BMI correlates with fat mass and free fat mass in colorectal cancer patients is multifactorial, involving pre-existing body composition, metabolic changes, the presence of nutritional interventions, and physical activity levels. Further study with a bigger population is encouraged.</p><p><b><i>Keywords:</i></b> colorectal cancer, BIA, BMI, fat mass, fat mass index, free fat mass, free fat mass index, cachexia</p><p><b>PP-03-139</b></p><p><b>Fat Mass and Free Fat Mass Index as a Determinant of Colorectal Cancer Stage Progression</b></p><p><b>Dewi Mustikarani</b> and Saskia Nursyirwan</p><p><i>Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The intricate relationship between body fat metrics, such as fat mass (FM) and free fat mass index (FFMI), and the progression of colorectal cancer (CRC) has garnered increasing interest. This study explores the association between FM and FFMI with cancer staging in a colorectal cancer population compared to non-cancerous patients in an Indonesian tertiary hospital.</p><p><b><i>Matherials and Methods:</i></b> This cross-sectional study utilized Bioelectrical Impedance Analysis (BIA) tools from Seca to assess body composition. Data were randomly collected from colorectal cancer outpatients between 2021 and 2024. A comparison group of non-CRC patients was also included. Statistical analysis was performed using SPSS version 25.</p><p><b><i>Results:</i></b> A total of 51 participants were included, with 25 diagnosed with colorectal cancer. The study revealed significant associations between FM and FFMI with the cancer stage (p=0.022 and p=0.001, respectively). Additionally, significant correlations were found between FM, FFMI, and the presence of cancer (p=0.000).</p><p><b><i>Conclusion:</i></b> Our findings demonstrate that FM and FFMI, as measured by Bioelectrical Impedance Analysis, are significantly associated with CRC staging. We also found a significant association between cancer and non-cancerous groups. This supports the hypothesis that cancer progression influences body composition. Further research with larger cohorts is recommended to validate these findings and explore the interplay between body composition, cancer stage, and other factors such as age and gender.</p><p><b><i>Keywords:</i></b> colorectal cancer, staging, Bioelectrical Impedance Analysis, fat mass, free fat mass index</p><p><b>PP-03-140</b></p><p><b>UGT1A1 Genotype and Irinotecan Efficacy in Colorectal Cancer: a Systematic Review</b></p><p><b>Cecilia Oktaria Permatadewi</b><sup>2</sup>, Kevin Tandarto<sup>1</sup>, Hery Djagat Purnomo<sup>2</sup>, Budi Setiawan<sup>3</sup>, Hesti Triwahyu Hutami<sup>2</sup>, Didik Indiarso<sup>2</sup>, Agung Prasetyo<sup>2</sup> and Hirlan Hirlan<sup>2</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia;</i> <sup>2</sup><i>Division of Gastroentero- Hepatology, Internal Medicine Department, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia;</i> <sup>3</sup><i>Division of Hematology and Medical Oncology, Internal Medicine Department, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Colorectal cancer (CRC) remains a significant health burden globally. Irinotecan, a key chemotherapeutic agent, is metabolized by UDP-glucuronosyltransferase 1A1 (UGT1A1). This systematic review aims to elucidate the impact of UGT1A1 genotype on irinotecan outcomes in CRC patients.</p><p><b><i>Methods:</i></b> A comprehensive search of Google Scholar, PubMed, Embase, and Cochrane Library databases was conducted following PRISMA 2020 guidelines. Studies assessing the association between UGT1A1 genotype and irinotecan response in CRC patients were included. Data extraction and quality assessment were performed independently by two reviewers.</p><p><b><i>Results:</i></b> A total of 572 articles were screened from the database. Published studies revealed a significant association between UGT1A1 *28 homozygosity and increased risk of severe irinotecan-induced toxicity. Additionally, UGT1A1 *28 homozygotes exhibited inferior progression-free survival and overall survival compared to wild-type or heterozygous individuals. Despite variations in study characteristics, the overall findings consistently supported the role of UGT1A1 *28 genotype in modulating irinotecan outcomes in CRC. Variations in UGT1A1 genotype, notably the *28 allele, have been implicated in irinotecan toxicity and efficacy discrepancies.</p><p><b><i>Conclusion:</i></b> Homozygosity for the *28 allele confers a higher risk of severe adverse events and poorer clinical outcomes. Testing for the UGT1A1 genotype allows for personalized dosing of irinotecan to minimize adverse effects and optimize therapeutic efficacy.</p><p><b>PP-03-142</b></p><p><b>Efficacy and safety of fecal microbiota transplantation in amytrophic lateral sclerosis</b></p><p>Jingshuang Yan, <b>Yunsheng Yang</b>, Jing Cui, Huixin Chen, Lihua Peng, Xiaoyang Lan and Shengyuan Yu</p><p><i>The First Medical Center, Chinese Pla General Hospital, Beijing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Amyotrophic lateral sclerosis(ALS) is a fatal, progressive neurodegenerative disease with unclear pathogenesis. The feasibility of fecal microbiota transplantation(FMT) for ALS with respiratory failure had been reported in two patients. Large clinical validation are needed.</p><p><b><i>Materials and methods:</i></b> A single-arm clinical trial was performed. ALS patients were enrolled to receive FMT by colonoscopy for three times with 6-week-interval. Evaluations were carried out one day before each FMT. The primary measure was the Revised ALS Functioning Rating Scale(ALSFRS-R), and response was defined as the score decreased by ≤ 0 points. Secondary measures were the ALS Assessment Questionnaire(ALSAQ-40), 36-Item Short Form Survey(SF-36), Self-rating Depression Scale(SDS) and Self-rating Anxiety Scale(SAS). Safety was assessed by the number and severity of adverse events.</p><p><b><i>Results:</i></b> Fifty-nine patients were recruited. A total of 34 patients were analyzed at the end point with 22 males and 12 females. The onset age was 52.88±10.20 years, disease duration of 23.12±15.84 months, ALSFRS-R of 36.97±7.36 at baseline.</p><p>Responses occurred in 55.88% of the patients after the first FMT, and 61.76% after the second FMT. The slope of ALSFRS-R was -0.60±0.50 points/month at baseline, -0.53±0.35 after the first FMT, and -0.50±0.34 after the second FMT. Measured by ALSFRS-R subitems, FMT treatment ameliorated the symptoms of bulbar and respiratory function.</p><p>The scores of ALSAQ-40, SF36, SDS also presented an improvement tendency. No adverse events were reported during the trial.</p><p><b><i>Conclusion:</i></b> FMT showed efficacy and safety for ALS with slower functional decline. Large double-blinded randomized controlled trials are needed to further verification.</p><p><b>PP-03-143</b></p><p><b>Dual therapy for type 2 diabetes H.pylori infection and the effect on the glycated hemoglobin</b></p><p><b>Xiaoyong Wang</b> and Xin Cao</p><p><i>The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Patients with diabetes are at a higher risk of failure of H. pylori eradication. This study aimed to evaluate the efficacy and safety of vonoprazan-amoxicillin (VA) dual therapy for the treatment of H. pylori infection in patients with T2DM and to assess the influence of H. pylori eradication on glycated hemoglobin A1C (A1C) level.</p><p><b><i>Materials and Methods:</i></b> This prospective, single-arm clinical trial enrolled 75 patients with T2DM diagnosed with H. pylori infection. Patients were treated with VA dual regimen comprising vonoprazan (20 mg twice daily) and amoxicillin (750 mg thrice daily) for 14 d (14-d VA dual therapy). 13C urea breath test was performed at least 4 weeks after completion of treatment. The A1C levels were measured 3 months post-treatment.</p><p><b><i>Results:</i></b> The eradication rates in the intention-to-treat analysis and per-protocol analysis were 84.0% (63/75) and 87.14% (61/70), respectively. Adverse events occurred in 10 patients (13.3%), with none of the patients developing any severe adverse event. Good compliance was noted in 93.3% (70/75) of patients. Cigarette smoking (P=0.011) and high A1C levels (P=0.019) were associated with failure to eradicate H. pylori. Patients in the successful eradication group showed a significant decrease in the A1C level 3 months post-treatment compared with pre-eradication level (7.70±1.05 vs 7.23±1.00%, P=0.006). The A1C levels in the treatment failure group showed no significant difference before and after treatment (8.06±0.61 vs 7.23±1.16%, P=0.117).</p><p><b><i>Conclusion:</i></b> VA dual therapy is a safe and effective regimen in patients with T2DM. Eradication of H. pylori improves glycemic control in these patients.</p><p><b>PP-03-144</b></p><p><b>1 / 2 Outcome predictors for vedolizumab therapy in patients with ulcerative colitis</b></p><p><b>Ayoung Cho</b></p><p><i>Sevrance Hospital, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Little is known about the prognostic factors related to clinical outcomes during vedolizumab therapy in ulcerative colitis patients. We aimed to investigate outcome predictors for vedolizumab during induction and maintenance therapy in UC</p><p><b><i>Method:</i></b> 73 patients with moderate to severe UC who received vedolizumab treatment at Severance-Hospital between 2017 and 2021 were included. The medical records of patients were retrospectively reviewed. For analysis, logistic regression analysis was used to analyze primary non-response during induction therapy, while Cox regression was used to analyze risk factors for relapse during maintenance therapy. Variables with a p-value < 0.1 in the univariate analysis were included in the multivariate analysis.</p><p><b><i>Results:</i></b> The average age was 43.6 ± 15.7 years, and 61.6% were male. After 14th week of drug administration, primary non-responders were identified in 15(20.5%), with high baseline CRP(> 6 mg/dl) identified as a risk factor(odds ratio 4.181, 95% confidence interval [CI] 1.028-17.013). Meanwhile, among the 41 who achieved clinical remission, clinical relapse occurred in 12%, 30%, 42% at 1year, 3years, 5years during maintenance. In multivariate analysis, vedolizumab dose intensification (hazard ratio 2.952, 95% CI 1.054-8.267) and absence of past anti-TNF use (hazard ratio 4.272, 95% CI 1.443-12.651) were identified as independent predictors of clinical relapse.</p><p><b><i>Conclusion:</i></b> UC patients who receiving vedolizumab, high CRP(> 6 mg/dl) was identified as a risk factor for primary non-response. Meanwhile, vedolizumab dose intensification and no previous anti-TNF use were risk factors for clinical relapse during maintenance. These prognosticators will be useful in selecting appropriate patients for vedolizumab treatment.</p><p><b>PP-03-145</b></p><p><b>Outcome predictors for vedolizumab therapy in patients with ulcerative colitis: A single-center retrospective cohort study</b></p><p><b>Ayoung Cho</b></p><p><i>Sevrance Hospital, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Little is known about the prognostic factors related to clinical outcomes during vedolizumab therapy in ulcerative colitis patients. We aimed to investigate outcome predictors for vedolizumab during induction and maintenance therapy in UC</p><p><b><i>Method:</i></b> 73 patients with moderate to severe UC who received vedolizumab treatment at Severance-Hospital between 2017 and 2021 were included. The medical records of patients were retrospectively reviewed. For analysis, logistic regression analysis was used to analyze primary non-response during induction therapy, while Cox regression was used to analyze risk factors for relapse during maintenance therapy. Variables with a p-value < 0.1 in the univariate analysis were included in the multivariate analysis.</p><p><b><i>Results:</i></b> The average age was 43.6 ± 15.7 years, and 61.6% were male. After 14th week of drug administration, primary non-responders were identified in 15(20.5%), with high baseline CRP(> 6 mg/dl) identified as a risk factor(odds ratio 4.181, 95% confidence interval [CI] 1.028-17.013). Meanwhile, among the 41 who achieved clinical remission, clinical relapse occurred in 12%, 30%, 42% at 1year, 3years, 5years during maintenance. In multivariate analysis, vedolizumab dose intensification (hazard ratio 2.952, 95% CI 1.054-8.267) and absence of past anti-TNF use (hazard ratio 4.272, 95% CI 1.443-12.651) were identified as independent predictors of clinical relapse.</p><p><b><i>Conclusion:</i></b> UC patients who receiving vedolizumab, high CRP(> 6 mg/dl) was identified as a risk factor for primary non-response. Meanwhile, vedolizumab dose intensification and no previous anti-TNF use were risk factors for clinical relapse during maintenance. These prognosticators will be useful in selecting appropriate patients for vedolizumab treatment.</p><p><b>PP-03-146</b></p><p><b>Efficacy and Safety of Mirikizumab in Ulcerative Colitis: A Systematic Review</b></p><p>Mohamed Karam Allah Elkholy<sup>1</sup>, Mazen Negmeldin Aly Yassin<sup>2</sup>, Dina Osama Yehia Ibrahim<sup>3</sup>, Duha Milad Abdullah Abuklish<sup>4</sup>, Amro Mamdouh Abdelrehim<sup>5</sup> and <b>Safia Elshennawy</b></p><p><sup>1</sup><i>College of Pharmacy Zagazig University, Zagazig, Egypt;</i> <sup>2</sup><i>College of Medicine, Cairo University, Cairo, Egypt;</i> <sup>3</sup><i>College of medicine Fayoum University, Fayoum, Egypt;</i> <sup>4</sup><i>Cardiology department, Tripoli hospital center, internal medicine;</i> <sup>5</sup><i>College of Medicine, Misr University for Science and Technology, Egypt</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ulcerative Colitis management is challenging. Despite the availability of several medication classes, about 15% of patients may still need colectomy. Mirikizumab, a novel monoclonal antibody directed against the p19 subunit of IL23 selectively, has demonstrated promising results in clinical trials. This review examines the efficiency and safety of mirikizumab in patients with moderate-to-severe active ulcerative colitis.</p><p><b><i>Materials and Methods:</i></b> Our literature search covered PubMed, Scopus, Web of Science, and Cochrane. We included only the clinical trials. The primary outcomes were clinical response, clinical remission, symptomatic remission, and safety profile.</p><p><b><i>Results:</i></b> Of the 394 studies identified, 4 were included. In the induction phases, evaluating efficacy at week 12, showed that 61% of patients achieved clinical response, 23% achieved remission, and 46% achieved symptomatic remission after intravenous mirikizumab at different doses every 4 weeks.</p><p>During the extended induction phases, evaluating efficacy at week 24, showed 52% clinical response, 11% clinical remission, and 38% symptomatic remission in patients who did not achieve a clinical response during the induction phases.</p><p>In the maintenance phases, evaluating efficacy at week 52, 73% of patients achieved clinical response, 45% achieved remission, and 66% achieved symptomatic remission with subcutaneous mirikizumab every 4 weeks.</p><p>The incidence of adverse events leading to discontinuation varied from 1.6% in the initial induction phases to 3.3% in the extended induction phases and 1.9% in the maintenance phases.</p><p><b><i>Conclusion:</i></b> This is the first systematic review of mirikizumab for UC patients. The results showed promising efficacy and acceptable tolerability, but further investigations are needed.</p><p><b>PP-03-147</b></p><p><b>Unhealing wound with enterocutaneous fistula as a presentation of Crohn's disease</b></p><p><b>Rendy Sidik</b> and Mario Stefanus and Muhammad Firhat Idrus</p><p><i>Division of Gastroenterology, Department of Internal Medicine University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Inflammatory Bowel Disease, in this case Crohn's disease(CD), has the potential to cause extraintestinal complications such as fistulas and the occurrence of colorectal cancer. Fistula complications increase morbidity and mortality, sepsis, and malnutrition. Fistulas formed in CD pose a higher and urgent management challenge, requiring a multidisciplinary approach.</p><p><b><i>Case Report:</i></b> A 52-year-old man was referred from a previous hospital with complaints of pus discharge from a non-healing wound on the right side of the abdomen for the last 2 weeks. Infected wound on the right side of the abdomen that comes and goes, accompanied by diarrhea 4-5 times a day for the past year. Incision drainage abscess surgery was done a year ago. Abdominal CT scan showed a localized abscess in the right abdomen region with fistulation with the ascending colon and the skin of the right abdomen wall. Colonoscopy revealed an irregular mass with ulcers and fistulation in the cecum area up to stenosis. Histological tissue examination showed high-grade dysplasia with negative TB PCR results. The patient was continued on antibiotic therapy and underwent surgery.</p><p><b><i>Discussion:</i></b> Chronic and recurrent abdominal wall wounds after surgery should be suspected of having inflammatory bowel disease, in this case, Crohn's disease. The prevalence of Crohn's disease with enterocutaneous fistula occurs in 10% of cases, although it commonly occurs in the perianal region. With optimal therapy using a combination of surgery and biologic agents, we hope to achieve a better total healing rate and reduce the risk of colorectal cancer.</p><p><b>PP-03-148</b></p><p><b>Clinical Outcomes and Prognostic Factors in Crohn’s Disease Patients with Internal Fistulas</b></p><p><b>Su Ji Woo</b>, Soo Jung Park, Jae Hee Cheon, Tae Il Kim and Jae Jun Park</p><p><i>Severance Hospital, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Although internal fistulas in Crohn's disease patients frequently result in complications and require surgical intervention, their outcome predictors are rarely known. This study investigated the long-term clinical outcomes and prognostic factors for CD patients with internal fistulas.</p><p><b><i>Methods:</i></b> Data were retrospectively collected from CD patients diagnosed with internal fistulas between March 2003 and August 2023. Clinical outcomes, including the occurrence of complications with or without surgical intervention and fistula closure, were assessed as primary endpoints. Statistical analysis methods, including Kaplan-Meier analysis with the log-rank test and Cox regression analysis, were used.</p><p><b><i>Results:</i></b> A total of 72 CD patients were included in the study, with a mean follow-up period of 1280 days. Fistula locations included entero-enteric or entero-colic (n=70, 97.2%), entero-vesical (n=2, 2.8%), and entero-vaginal (n=2, 2.8%). Regarding complications with or without surgical intervention, occurrences were 34.6% at one year, 53.0% at three years, and 54.7% at five years. Multivariate Cox analysis showed that immunomodulator use reduced the occurrence of complications (hazard ratio 0.395, 95% confidence interval [CI] 0.207-0.754), while a history of previous bowel resection was associated with an increased risk of complications (hazard ratio 1.874, 95% CI 1.001-3.516). Meanwhile, among 56 Crohn's disease patients with internal fistulas treated with immunomodulators or anti-TNF agents, fistula closure was confirmed in 10 patients (17.9%) during the follow-up period.</p><p><b><i>Conclusion:</i></b> The use of immunomodulators was associated with a reduction in complications. Our results suggest that close monitoring is necessary, and that the appropriate use of immunomodulators can be beneficial in these patients.</p><p><b>PP-03-149</b></p><p><b>Fecal siderophore genes are potential biomarker for ulcerative colitis</b></p><p>Jingshuang Yan, Rongrong Ren, Zhengpeng Li, Wanyue Dan, Xiaohan Zhang, Xiaoyan Chi, Lihua Peng and <b>Yunsheng Yang</b></p><p><i>The First Medical Center, Chinese PLA General Hospital, Beijing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Gut microbiome-related biomarkers have considerable potential for disease assessment and therapeutic guidance in UC. This study aims to explore the potential value of fecal siderophore genes as a non-invasive biomarker to evaluate UC activity.</p><p><b><i>Methods:</i></b> We included 166 patients with active UC (37 mild, 83 moderate, and 46 severe cases) and 165 healthy controls (HCs). Quantitative real-time PCR were used for the detection of eight siderophore genes, including enterobactin (entF, fepA), salmochelin (iroB, iroN), aerobactin (iucA, iutA), and yersiniabactin (irp1, fyuA).</p><p><b><i>Results:</i></b> Patients with UC showed higher siderophore gene total copy number than HCs (1182.49 vs. 176.44 copies/ng). A threshold of 2877.68 copies/ng distinguished patients with active UC from HCs, with 90.3% specificity and 33.1% sensitivity.</p><p>The total copy number of siderophore genes was significantly higher in patients with severe active UC than in those with moderate (3111.03 vs 1183.54 copies/ng) and mild (3111.03 vs 672.60 copies/ng) active UC. Patients with severe endoscopic activity also exhibited higher total copy number than that in patients with mild-to-moderate endoscopic activity (1318.02 vs. 723.10 copies/ng). A threshold of 10298.63 copies/ng achieved 92.5% specificity and 43.5% sensitivity in identifying severe active UC, and 94% specificity and 22.5% sensitivity in identifying endoscopic severe active UC. In parallel test with C-reactive protein, the sensitivity and specificity increased to 89.1% and 75.8% for the clinical severe active stage.</p><p><b><i>Conclusion:</i></b> Total siderophore gene copy number was positively correlated with clinical and endoscopic disease activity in UC, and serve as a potential non-invasive biomarker for UC activity.</p><p><b>PP-03-150</b></p><p><b>Fecal siderophore gene potentially predict the responsiveness of fecal microbiota transplantation for active ulcerative colitis</b></p><p>Jingshuang Yan, Guanzhou Zhou, Rongrong Ren, Xiaohan Zhang, Zikai Wang, Lihua Peng and <b>Yunsheng Yang</b></p><p><i>The First Medical Center, Chinese Pla General Hospital, Beijing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Predictive markers for the outcomes of fecal microbiota transplantation (FMT) in ulcerative colitis (UC) are poorly defined. We aimed to explore the potential value of siderophore genes as non-invasive biomarker for predicting FMT responsiveness.</p><p><b><i>Materials and methods:</i></b> We enrolled patients with active UC (Mayo score ≥ 3) who underwent two FMT procedures. Fecal samples were collected before and 8 weeks after each FMT session and determined the total copy number of eight siderophore genes, including enterobactin (entF, fepA), salmochelin (iroB, iroN), aerobactin (iucA, iutA), and yersiniabactin (irp1, fyuA). The relationship between total siderophore genes copy number and FMT efficacy was analyzed.</p><p><b><i>Results:</i></b> Seventy-two patients with UC underwent FMT. The UC clinical response and remission rates were 62.5% and 19.4% after the first FMT, which increased to 71.7% and 43.4%, respectively, after the second FMT. Compared with baseline, the total siderophore genes copy number significantly decreased in the responder group, from 1557.32 copies/ng to 251.90 copies/ng after the second FMT. In the non-responder group, the total copy number of siderophore genes showed an increasing trend after the second FMT, from 65.46 copies/ng to 330.78 copies/ng. The total baseline copy number was significantly higher in responders than in non-responders (P < 0.01). A baseline total copy number cutoff value of 289.63 copies/ng showed 85.5% sensitivity and 88.2% specificity in predicting FMT responsiveness.</p><p><b><i>Conclusion:</i></b> The total copy number of fecal siderophore genes in patients with UC correlates with FMT response, providing a promising biomarker for predicting FMT responsiveness.</p><p><b>PP-03-151</b></p><p><b>The swollen gut: a case report of gastrointestinal amyloidosis</b></p><p><b>Kunhan Chiam</b></p><p><i>Tan Tock Seng Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Amyloidosis is an infiltrative disorder due to extracellular deposits of amyloid fibrils. They distort the tissue architecture and function. Incidence is 1 case per 100000 person-years in Western countries and classified into: Systemic and localized amyloidosis. We report a patient with amyloidosis localized to the GI tract complicating with malabsorption, hypoalbuminemia, and anasarca.</p><p><b><i>Case Description:</i></b> A 64 year old male presented with 9 months of lower limb swelling, bloating, and found to have iron deficiency anemia with hypoalbuminemia. Computed tomography imaging showed thickening throughout the small bowel loops from duodenum to terminal ileum, with pleural effusions. Endoscopy showed swelling and friable mucosa in the duodenum, and transverse colon. Biopsies were taken and histology of the duodenum and transverse colon showed deposition of extracellular, amorphous eosinophilic materials in the lamina propria, and Congo red stains of the eosinophilic material exhibit apple green birefringent staining consistent with amyloidosis. Bone marrow aspirate with trephine, and abdominal fat pad biopsy were done which did not show any amyloid deposits. This was conclusive of localized GI amyloidosis without systemic involvement.</p><p><b><i>Relevant investigations:</i></b> Hb 5.9g/dL, Ferritin 4ug/L, albumin <15 g/L</p><p><b>PP-03-152</b></p><p><b>Achalasia or not Achalasia-Is it as simple as we think ?</b></p><p><b>Jay Chudasama</b></p><p><i>Topiwala National Medical College, Mumbai, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>70 years old male presented with Difficulty in swallowing(Solid+, Liquid +) with Retrosternal pain without regurgitation, weight loss and any significant past history since 6 month.(Ekcardt score - 4)Upper GI scopy showed Normal study.36 channel solid state HRM showed Median IRP 16.9 mm Hg with premature peristalsis with compartmental pressurization in >50 % swallows. Impression was given EGJ outflow obstruction with compartmentalized pressurization. HRCT thorax was normal. Trial of PPI given and repeat HRM was advised after 6 months. Took PPI for 2 months but</p><p>Symptoms were persistent. HRM(36 channel water perfusion)preformed, On wet swallows, showed mean IRP of 10 wet swallows -10 mm Hg, LES pressure – 14.1 mm Hg, 40 % swallows showed Ineffective peristalsis(DCI <450).On cookie swallow mean was IRP -9 mm Hg, 100% swallowed showed ineffective peristalsis(DCI < 450).Rapid drink challenge(supine position) showed median IRP-14.9 mm Hg, LES pressure – 42 mm Hg, presence of Pan esophageal pressurization. Timed barium esophagogram showed Smooth narrowing at distal esophagus with 11 cm height of barium column at 2 minutes s/o outflow obstruction/achalasia. Achalasia Cardia type II(In evolution) diagnosis was made and managed with 35 mm pneumatic balloon dilatation[Ekcardt score – 0 (2 weeks post dilatation ),symptom free].EGJ outflow obstruction can be found in normal individuals, persistent symptoms should be thoroughly investigated. Provocative tests can be helpful in a diagnosis of inconclusive manometric findings. Supportive tests like TBE/EndoFLIP should be performed in such cases. Pneumatic balloon dilatation should be used to manage dysphagia in doubtful cases of achalasia.</p><p><b>PP-03-153</b></p><p><b>Gastric amyloidosis- a case report</b></p><p><b>Md. Musab Khalil</b></p><p><i>Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b>Abstract:</b></p><p><b><i>Introduction:</i></b> Amyloidosis are abnormal misfolded proteins that are resistant to degradation. Due to defective metabolism, they may be deposited in various organs causing organ dysfunction. Localized amyloidosis is a rare disorder.</p><p><b><i>Case Presentation:</i></b> We are describing a case of isolated Gastric amyloidosis who presented with recurrent hematemesis and melena due to gastric ulcers. Gastroscopy revealed multiple gastric ulcers of variable sizes and shapes. Biopsy followed by histopathology with Congo red staining revealed Gastric amyloidosis.</p><p><b><i>Conclusion:</i></b> Amyloidosis is a rare disorder. Localized amyloidosis is much rarer compared to systemic amyloidosis. Compared to systemic amyloidosis, localized amyloidosis has a better prognosis. High level of suspicion is needed to diagnose amyloidosis as a rare case of gastric ulcer.</p><p><b>PP-03-154</b></p><p><b>A case of phlebosclerotic colitis</b></p><p><b>Fujuan Luan</b></p><p><i>The First Affiliated Hospital Of Soochow University, Suzhou, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> A man was admitted due to recurring abdominal pain present for 4 months. After undergoing an abdominal CT scan and colonoscopy, he was diagnosed with phlebosclerotic colitis (PC). Subsequent subtotal colectomy and postoperative pathological analysis confirmed this diagnosis.</p><p><b><i>Case Description:</i></b> A 71-year-old man was admitted due to recurrent abdominal pain in the lower right quadrant for a period of four months. Physical examination revealed tenderness in the lower right quadrant without rebound. Abdominal CT scan showed thickening of the wall in the ascending colon and hepatic flexure, with multiple \"thread-like\" calcifications observed in both the colon wall and adjacent mesenteric area. Colonoscopy revealed dark bluish mucosa in the ascending colon and edematous erosive mucosa in the transverse colon. Despite two weeks of conservative treatment, the patient's abdominal pain did not relieve, leading to consideration of exploratory laparotomy. During surgery, we discovered a dark blackened colon wall extending from cecum to descending colon, ultimately resulting in subtotal colectomy being performed. Pathological examination revealed extensive fibrosis and scattered calcification within both submucosal and serosal layers. The venous walls within these layers exhibited thickening along with diffuse fibrosis, hyaline degeneration, and calcification.</p><p><b><i>Discussion:</i></b> PC also known as idiopathic mesenteric venous sclerosis or idiopathic mesenteric venous enteritis, is characterized by extensive calcification of the branch of the superior mesenteric vein and the wall of the colonic vein, along with thickening of the right half of the colonic wall. PC is a type of ischemic colitis that is non-thrombotic and non-embolic.</p><p><b>PP-03-155</b></p><p><b>Protective effect of 1,2-propanediol on radiation-induced intestinal injury in mice</b></p><p><b>Jiwei Zhao</b> and Gang Sun</p><p><i>Chinese PLA General Hospital, Beijing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to investigate the protective effect of 1,2-propanediol on radiation-induced intestinal injury in mice.</p><p><b><i>Materials and Methods:</i></b> 1、Mice were divided into an irradiation group and a drug administration group. The proliferation and regeneration of crypts were observed, and the intestinal damage was assessed by measuring the length of intestinal villi. The 30-day survival rate was also observed.</p><p>2、Immunohistochemistry of Olfm4 and Lysozyme, along with immunofluorescence using Lgr5-EGFP-IRES-CRE/ERT2 mice, was employed to detect the survival, proliferation, and regeneration of intestinal stem cells (ISCs).</p><p><b><i>Results</i></b>: 1、The number of regenerated crypts in the irradiation group sharply decreased from 135.06±6.72 to 18.73±3.49. Compared with the irradiation-only group, the drug administration group showed an increase in the number of crypts and a significant increase in villus length, with a 30-day survival rate of 100%.</p><p>2、In the irradiation group, the number of Olfm4+ cells in the intestinal crypts decreased. Compared with the control group, the 1,2-PD administration group showed a significant reduction in Olfm4+ cells within 48 hours. After 3.5 days, proliferation and regeneration increased, inhibiting the loss of Paneth cells, and the proliferation of Lgr5-EGFP+ISCs increased.</p><p><b><i>Conclusion:</i></b> 1,2-Propanediol has a protective effect against radiation-induced intestinal injury.</p><p><b>PP-03-156</b></p><p><b>1,2-propanediol ameliorated radiation-induced intestinal injury in mice via regulating P53</b></p><p><b>Jiwei Zhao</b><sup>1</sup>, Gang Sun<sup>1</sup> and Zuyin Yu<sup>2</sup></p><p><sup>1</sup><i>Chinese PLA General Hospital, Beijing, China;</i> <sup>2</sup><i>Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aims to investigate the protective effect of 1,2-propanediol (1,2-PD) on radiation-induced intestinal injury in mice via the P53 pathway.</p><p><b><i>Materials and Methods</i></b>: IEC-6 cells from both the irradiation group and the 1,2-PD-treated group were collected at 0 h, 3 h, 6 h, 12 h, and 24 h, and PI staining was performed to examine the cell cycle status. After 24 hours of incubation with different concentrations of 1,2-PD, proteins were extracted for immunoblotting experiments to screen for molecular targets related to cell cycle regulation.</p><p>Total protein and RNA were extracted from intestinal crypt cells of mice at 6 h and 24 h post-irradiation. Protein immunoblotting and RT-qPCR assays were conducted to detect molecules related to the P53-PUMA pathway. Additionally, survival observations and intestinal BrdU immunohistochemical detection were performed using P53-/- mice.</p><p><b><i>Results</i></b>: After 12 hours of 1,2-PD administration, IEC-6 cells exhibited cell cycle arrest. With increasing concentrations of 1,2-PD, phosphorylation at the Rb S87/811 sites of the cell cycle regulatory protein Rb showed a gradient inhibition effect, leading to the dephosphorylation of Rb protein into an active state, which inhibits cell cycle progression.</p><p>Administration of 1,2-PD significantly inhibited the expression of genes and proteins related to the P53 pathway, as well as their downstream target proteins such as PUMA, Bax, and P21, at 6 hours and 24 hours post-irradiation.</p><p><b><i>Conclusion</i></b>: 1,2-Propanediol exerts protective effects against radiation-induced intestinal injury by inhibiting apoptosis of crypt cells mediated by the P53-PUMA pathway</p><p><b>PP-03-157</b></p><p><b>Ascitic fluid and serum homocysteine as a potential biomarker in spontaneous bacterial peritonitis: A meta-analysis</b></p><p><b>Rukesh Yadav</b></p><p><i>Maharajgunj Medical Campus, Institute Of Medicine, Tribhuvan University, Kathmandu, Nepal</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> A polymorphonuclear leukocyte cell count of greater than 250/mm³ is the primary diagnostic criterion for spontaneous bacterial peritonitis (SBP). This approach is invasive and may not be diagnostic for all SBP variants. Accessible biomarkers are therefore required for the diagnosis of SBP. The purpose of the meta-analysis was to evaluate the potential diagnostic utility of homocysteine for SBP.</p><p><b><i>Materials and Methods:</i></b> From the inception to January 2024, the Pubmed, Google Scholar, and Embase were searched for studies that assessed homocysteine concentrations of cirrhotic patients with and without SBP in the serum and the ascitic fluid. Results comprised the mean difference (MD) in the homocysteine concentrations in the serum and ascitic fluid of the two groups with a 95% Confidence Interval (CI). According to the heterogeneity, the homocysteine values were analyzed using the Revman 5.1 program using either a fixed or random effect.</p><p><b><i>Results:</i></b> In this meta-analysis, four studies with 533 cirrhotic patients were included. 182 had SBP and 351 did not have SBP. Based on three studies, the analysis revealed a significantly higher ascitic fluid homocysteine in SBP patients as compared to non-SBP patients (MD: 3.25, CI: 2.26-4.23, I²=78%, P value <0.00001, figure 1) Furthermore, serum homocysteine in SBP patients was significantly higher than the non-SBP patients (MD: 6.22, CI: 4.47-7.96, I²=0%, P value <0.00001).</p><p><b><i>Conclusions:</i></b> This meta-analysis shows that patients with SBP have significantly higher serum and ascitic homocysteine levels than individuals without SBP. For any SBP variants, serum homocysteine may offer a trustworthy and noninvasive diagnostic value.</p><p><b>PP-03-158</b></p><p><b>MAFLD in Older Adults is Associated with Atrial Fibrillation and Physical Disability But Not Mortality</b></p><p><b>Daniel Clayton-Chubb</b><sup>1,2</sup>, Stuart Roberts<sup>1,2</sup>, Ammar Majeed<sup>1,2</sup>, Robyn Woods<sup>2</sup>, Andrew Tonkin<sup>2</sup>, Mark Nelson<sup>3</sup>, Andrew Chan<sup>4</sup>, Joanne Ryan<sup>2</sup>, Ms Cammie Tran<sup>2</sup>, Alexander Hodge<sup>2</sup>, John Lubel<sup>1,2</sup>, Hans Schneider<sup>1,2</sup>, Amy Brodtmann<sup>2</sup>, John McNeil<sup>2</sup> and William Kemp<sup>1,2</sup></p><p><sup>1</sup><i>Alfred Health, Melbourne, Australia;</i> <sup>2</sup><i>Monash University, Melbourne, Australia;</i> <sup>3</sup><i>University of Tasmania, Australia;</i> <sup>4</sup><i>Massachusetts General Hospital, Boston, USA</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The impact of Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) on older adults is understudied particularly in relation to cardiovascular disease-related outcomes. Therefore, we aimed to evaluate the impact of MAFLD on key health outcomes for older adults using data from the ASPirin in Reducing Events in the Elderly (ASPREE) trial and ASPREE-XT cohort.</p><p><b><i>Materials and Methods:</i></b> ASPREE included 16,703 community-dwelling Australian participants aged ≥70 years without cardiovascular disease, dementia, or independence-limiting physical disability; 9,846 had complete data for this analysis. Anthropometric, biochemical, and questionnaire data were collected at baseline. Hepatic steatosis (HS) was defined as a Fatty Liver Index (FLI) score ≥ 60. MAFLD was defined as HS along with usual cardiometabolic criteria. FLI < 30 was used as a no-HS comparator.</p><p><b><i>Results:</i></b> Of the 9,846, 38.0% (3,742) had MAFLD (mean age 75.0 ± 4.2 years, 53.2% female). MAFLD is strongly associated with persistent physical disability (aHR 1.46 [95% CI 1.19 – 1.80]) and atrial fibrillation (aHR 1.44 [95% CI 1.11 – 1.86]). While it’s associated with major adverse cardiovascular events (MACE) on unadjusted analyses (HR 1.42 [95% CI 1.17 – 1.71]), this association is lost when fully adjusted (aHR 1.18 [95% CI 0.95 – 1.47]). MAFLD is not associated with mortality (aHR 1.03 [95% CI 0.88 – 1.20]).</p><p><b><i>Conclusion:</i></b> MAFLD is common in older adults, is related to atrial fibrillation, MACE, and physical disability, but does not confer an excess risk of all-cause mortality. These data have important public health implications.</p><p><b>PP-03-159</b></p><p><b>Disease Prevalence and Current Therapies for Metabolic Dysfunction-Associated Steatotic Liver Disease in India</b></p><p><b>Geeta S Desai</b><sup>1</sup>, Sandesha Ghorpade<sup>2</sup> and Santosh Hajare<sup>3</sup></p><p><sup>1</sup><i>Department of Gastroenterology JNMC, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India;</i> <sup>2</sup><i>Department of Gastroenterology, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India;</i> <sup>3</sup><i>H.O.D., Department of Gastroenterology, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The increasing global prevalence of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD), requires effective treatments. Saroglitazar, Vitamin E, Omega-3 fatty acids, and lifestyle modifications have shown varying effectiveness. This study aims to determine MASLD prevalence and evaluate treatment efficacy in North Karnataka.</p><p><b><i>Methods:</i></b> From 2021, patients aged 18-55 without liver disease history were screened on various parameters excluding alcoholic and viral causes. Fatty liver on imaging indicated MASLD/MASH. We assessed treatments (Diet and Exercise, Saroglitazar, Vitamin E, Omega-3 fatty acids) in 100 MASLD/MASH patients in 1:1:1:1 ratio. Liver stiffness, transaminases, and triglycerides were monitored at baseline, 24 weeks, and 52 weeks.</p><p><b><i>Results:</i></b> Total 3,348 patients screened, 1410 were diagnosed with MASLD/MASH. Among 1,026 analyzed patients, 12.34% had abnormal liver function tests, 45.53% had abnormal CAP, 27.23% had abnormal LSM, and 14.89% had abnormal LFTs. Among MASLD/MASH patients, 17.58% had diabetes and 2.55% had lean MASH. Among Treatment group age averages (in years): Diet and Exercise 43.04 ± 7.31, Saroglitazar 47.73 ± 6.52, Vitamin E 40.44 ± 8.03, Omega-3 FAs 41.72 ± 10.75. Over 52 weeks, liver stiffness changes were: Saroglitazar -5.18 KPa (-42.91%, P=0.001), Vitamin E -2.12 KPa (-19.40%, P=0.001), Diet and Exercise -1.33 KPa (-11.88%, P=0.001), Omega-3 FAs -0.45 KPa (-4.24%, P=0.342). Significant improvements in transaminases and triglycerides were noted for Saroglitazar and Vitamin E. No adverse events were reported.</p><p><b><i>Conclusion:</i></b> The 42.11% prevalence of MASLD and MASH in North Karnataka underscores the urgent need for lifestyle awareness. Saroglitazar effectively improves liver health markers in MASLD, warranting further investigation.</p><p><b>PP-03-160</b></p><p><b>New Onset Metabolic Syndrome Associated with MAFLD in A Young Adult: A Case Report</b></p><p><b>Yovita Gotama</b>, Anak Agung Istri Kumala Dewi, I Made Suma Wirawan and Made Wirama Diyana</p><p><i>Department of Internal Medicine, Wangaya Regional Hospital, Denpasar, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Metabolic-associated fatty liver disease (MAFLD) is increasingly recognized as a significant health issue linked to metabolic dysfunction – with an estimated global prevalence of 37%. Metabolic syndrome is a cluster of conditions, including obesity, insulin resistance, hypertension, and dyslipidemia. This case report explores a new onset of MAFLD in young adults diagnosed with metabolic syndrome, emphasizing the need for early recognition and intervention.</p><p><b><i>Case Description:</i></b> A 33-year-old female with intermittent right upper abdominal pain. She has a medical history of well-controlled hypertension, but no history of diabetes or family history of diabetes. No history of alcoholism. The patient had a BMI of 26.6 kg/m2, categorized as grade I obesity, and a waist circumference of 100 cm. Laboratory tests revealed a fasting blood glucose of 240 mg/dL, HbA1C of 9.8%, HDL of 28 mg/dL, and triglycerides of 339 mg/dL. Liver function test results AST 48 (0-37) U/L and ALT 55 (0-42) U/L. USG examination showed a grade III fatty liver, cholelithiasis, and cholecystitis. The medication includes diet and healthy lifestyle, long-acting insulin combination with antidiabetic drugs, antihypertension, antibiotics, fibrate, and ursodeoxycholic acid.</p><p><b><i>Discussion:</i></b> The patient presented with metabolic syndrome and liver function tests and USG results confirmed MAFLD. This case highlights MAFLD manifesting in younger populations, generally considered at lower risk. The mechanisms linked to insulin resistance, chronic inflammation, and lipid metabolism dysfunction. It is essential to assessed metabolic status and implementation of lifestyle interventions and medical management. Early intervention is needed to manage and mitigate the progression of MAFLD.</p><p><b>PP-03-161</b></p><p><b>Plasma Proteomic Signatures revealed altered Lipid/cholesterol metabolism, Coagulation and Complement pathways associated with Nonalcoholic steatohepatitis</b></p><p><b>Abhishak Gupta</b><sup>1,2,3</sup>, Puja Sakhuja<sup>2</sup> and Shiv K Sarin<sup>3</sup></p><p><sup>1</sup><i>Artemis Hospitals, Gurugram, India;</i> <sup>2</sup><i>G B Pant Hospital, DELHI, India;</i> <sup>3</sup><i>Institute of Liver and Biliary Sciences, Delhi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Fat accumulates in the liver and serves as a defining feature of non-alcoholic fatty liver disease (NAFLD). Plasma is a reflection of the body's metabolic changes and a useful tool for identifying biomarkers in the disease state. Objectives: We aimed to standardize low abundance plasma proteins in order to find possible biomarkers for NAFLD/NASH.</p><p><b><i>Methods:</i></b> Plasma proteomic profiles of a total of 74 plasma (25controls+54 NAFLD/NASH) were analyzed after depleted major proteins (globulins and albumin) using two-dimensional gel electrophoresis (2D-Gel) and coupled with MALDI-TOF. The differentially expressed proteins between the comparable groups were quantified and validated by ELISA.</p><p><b><i>Results:</i></b> After depleting major plasma proteins, we observed ≈300 proteins on 2D-Gel electrophoresis. Sixty differentially expressed (2-4 folds) protein spots were identified by the Mascot search database. Our integrated analysis show seven (n=7) significantly different plasma proteins (p<0.05), such as Transferrin(TF), Transthyretin(TTR), Complement C3, Fatty acid binding protein(FABP), Apolipoprotein-A1(APO-A1), Haptoglobin(Hp) and Fibrinogen(FBG) were involved in lipid/cholesterol metabolism, coagulation and complement pathways in NASH patients. Two-proteins, TF and TTR, were down-regulated and others were up-regulated in NASH. Further, plasma A-FABP level was significantly higher in NAFLD patients as compared with controls (47.3±14.8 vs. 13.7±9.8ng/ml; p<0.001). We also observed significant correlation between circulating A-FABP levels and BMI in the subgroup of NAFLD patients who had BMI≥25 kg/m2(n=36,74%) as compared with those BMI≤25kg/m2 (n=18,26%)(42.4±19.2vs. 33.7±15.2ng/ml;P<0.01), respectively.</p><p><b><i>Conclusions:</i></b> Plasma proteomic signatures reflect altered coagulation/complement pathway, cholesterol/lipid metabolism in NASH patients. A-FABP may also be a useful biomarker for prognosis and assessing disease severity.</p><p><b>PP-03-162</b></p><p><b>Correlation of Non-Invasive Fibrosis Scores with Vibration-Controlled Transient Elastography in MASLD Patients</b></p><p><b>KK Rashid</b>, Jesse Jacob Skariah, S Sreekumar, A Shanid, Srijaya S Sreesh and Krishnadas Devadas</p><p><i>Government Medical College, Thiruvananthapuram, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Fibrosis is a key indicator of disease severity in Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD). Non-invasive tests (NITs) offer safer, more convenient alternatives to liver biopsy for assessing fibrosis. This study compares the NAFLD Fibrosis Score (NFS), Fibrosis-4 (FIB-4) index, and Aspartate Aminotransferase to Platelet Ratio Index (APRI) against Vibration-Controlled Transient Elastography (VCTE) in MASLD patients.</p><p><b><i>Materials and Methods:</i></b> This retrospective observational study included 1169 MASLD patients who underwent VCTE. Fibrosis was staged by the APASL (Asian Pacific Association for the Study of the Liver) grading system based on VCTE. APRI, NFS, and FIB-4 index were calculated. The diagnostic performance of APRI, NFS, and FIB-4 in identifying significant fibrosis and above was assessed by calculating the area under the receiver operating characteristic curve (AUROC).</p><p><b><i>Results:</i></b> Among the patients, 643 (55%) were males. Fibrosis stages were F0-F1 (65%), F2 (11%), F3 (16%), and F4 (8.2%). Correlation analysis between VCTE and other parameters, including APRI, FIB-4, and NFS, showed statistically significant correlations (Pearson correlation coefficients, r= 0.349, 0.475, and 0.542, respectively). ROC curve analysis demonstrated the predictive capability of various parameters for fibrosis (VCTE > 8.2), with AUROC values of 0.713 for APRI, 0.799 for FIB-4, and 0.852 for NFS.</p><p><b><i>Conclusion:</i></b> Non-invasive scoring systems like APRI, FIB-4, and NFS are effective bedside tools for diagnosing liver fibrosis in MASLD patients. These tests offer a streamlined approach to diagnosis, enhancing patient care by reducing the need for invasive procedures in MASLD</p><p><b>PP-03-163</b></p><p><b>Investigating the Relationship Between Hepatic Steatosis and Fibrosis in Chronic Hepatitis B Patients</b></p><p><b>K Cokorde Istri Yuliandari Krisnawardani</b><sup>1</sup>, Ketut Mariadi<sup>2</sup>, Gde Somayana<sup>2</sup> and Komang Agus Wira Nugraha<sup>2</sup></p><p><sup>1</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Denpasar, Indonesia;</i> <sup>2</sup><i>Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> With the rising prevalence of obesity, hepatic steatosis often coexists with chronic hepatitis B (CHB) infection and may influence liver-related outcomes. This study aims to investigate the effect of hepatic steatosis on fibrosis progression in CHB patients.</p><p><b><i>Materials and Methods:</i></b> This cross-sectional, single-center study included 303 CHB patients. Exclusions were chronic hepatitis C, alcoholic liver disease, hepatocellular carcinoma, and autoimmune liver disease. Anthropometric measurements (weight and height) were taken. Liver stiffness and controlled attenuation parameter (CAP) for hepatic steatosis were measured using transient elastography. Liver fibrosis was defined as TE stage F2-4, and hepatic steatosis and severe steatosis were defined as CAP ≥248 dB/m and ≥280 dB/m, respectively. Appropriate statistical analyses were conducted.</p><p><b><i>Results:</i></b> The median age of 303 CHB patients (63.7% male) was 49 years old. Hepatic steatosis prevalence was 29.7%. Patients with steatosis had a higher BMI than those without (p<0.001). There was no significant difference in liver fibrosis between patients with and without steatosis (9.9 kPa vs. 10.41 kPa, p=0.371), even with increasing steatosis severity (p=0.452). Some patients in this study had already on nucleoside analogs therapy for a median duration of 6 months, yet no difference in fibrosis was found between the treated and untreated groups (9.2 kPa vs 11.9 kPa, p=0.941). Multivariate logistic regression showed increasing age and male sex were independently associated with liver fibrosis (AOR: 1.04, 95% CI: 1.01-1.06, p<0.001 and AOR: 3.41, 95% CI: 1.97-5.93, p<0.001 respectively).</p><p><b><i>Conclusion:</i></b> The presence of hepatic steatosis was not associated with fibrosis in CHB patiens.</p><p><b>PP-03-164</b></p><p><b>Probiotics Improve Liver Function in Non-Alcoholic Fatty Liver Diseases: A Systematic Review and Meta-Analysis</b></p><p><b>Meti Metiani</b><sup>1</sup>, Idn Wibawa<sup>2</sup> and Hasani Farhan<sup>3</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Hermina Arcamanik Hospital, Bandung, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Balimed Hospital, Denpasar, Indonesia;</i> <sup>3</sup><i>Bandung Islamic University, Bandung, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This review aimed to systematically analyze the effect of probiotics on improving liver function in NAFLD treatment.</p><p><b><i>Methods:</i></b> The authors searched for best quality studies (RCTs) up to 1 January, 2012 in 3 databases (Pubmed, Cochrane Library, and Google Scholar) on administering probiotics to NAFLD patients. The risk of bias was assessed and the results were combined using specialized software. Mean differences between groups were analyzed with 95% confidence intervals. Heterogeneity between studies was examined to determine appropriate statistical models. Results are presented visually and interpreted with a significance level of p<0.05</p><p><b><i>Results:</i></b> We analyzed 14 studies with 841 participants. The mean differences (MD) for the benefits of probiotics to reduce ALT were -3.05 (confidence interval [CI] -5.00, -1.10), to reduce AST were -0.56 (confidence interval [CI] -2.21, 1.09), to reduce GGT were -0.64 (confidence interval [CI] -163, 036), and to reduce ALP were 0.34 (confidence interval [CI] -4,99, 5.67). In addition, the benefits of probiotics on inflammatory markers were tested with TNF alpha were 0.46 (confidence interval [CI] -0.88, 1.81) and IL-6 were 0.42 (confidence interval [CI] -0.13, 0.96).</p><p><b><i>Conclusion:</i></b> Probiotics improved liver enzyme values and inflammatory markers in NAFLD, but only the reduction in ALT levels was statistically significant.</p><p><b>PP-03-165</b></p><p><b>Probiotics as a Potential Treatment for Non-Alcoholic Fatty Liver Diseases: A Systematic Review and Meta-Analysis</b></p><p><b>Meti Metiani</b><sup>1</sup>, I Dewa Nyoman Wibawa<sup>2</sup> and Hasani Farhan<sup>3</sup></p><p><sup>1</sup><i>Department of Internal Medicine, Hermina Arcamanik Hospital, Bandung, Indonesia;</i> <sup>2</sup><i>Department of Internal Medicine, Balimed Hospital, Denpasar, Indonesia;</i> <sup>3</sup><i>Bandung Islamic University, Bandung, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To evaluate the effect of probiotics as a potential treatment for non-alcoholic fatty liver disease (NAFLD).</p><p><b><i>Materials and Methods:</i></b> A systematic search was conducted in PubMed, Cochrane Library, and MEDLINE Complete for randomized control trials (RCTs) of probiotics in patients with NAFLD from 2011-2024. The results were meta-analyzed using RevMan 5.4 software and the Cochrane Risk-of-Bias 2 tool.</p><p><b><i>Results:</i></b> The study included 18 RCTs with 865 patients diagnosed with NAFLD. The results showed that the administration of probiotics reduced liver enzyme levels; although only ALT (MD = −1.65, 95% CI [-2.39, -0.91], P < 0.0001) and GGT (MD = −5.81, 95% CI [-7.68, -3.93], P < 0.00001) were significantly decrease. Probiotics were found to decrease liver steatosis; however, the reduction was only significant for grade 1 (OR = 0.49, 95% CI [0.27, 0.89], P = 0.02) and grade 2 (OR = 0.33, 95% CI [0.18, 0.61], P = 0.0004). Improvement in liver fibrosis was assessed by transient elastography (Fibroscan) (MD = -0.65, 95% CI [-1.10, -0.20], P = 0.005), and probiotics were also shown to reduce levels of inflammatory markers; however, only TNF-α (MD = -1.46, 95% CI [-1.86, -1.07], P < 0.00001) and IL-6 (MD = −0.94, 95% CI [-1.30, -0.58], P < 0.00001) showed a significant decrease.</p><p><b><i>Conclusion:</i></b> Probiotics have been shown to have a positive effect on NAFLD by normalizing liver enzyme, improving fibrosis, and reducing steatosis and inflammatory markers. Further study is needed to confirm the effect of probiotics and evaluate long-term efficacy and safety.</p><p><b>PP-03-166</b></p><p><b>Lifestyles associated with MASLD</b></p><p><b>Hirofumi Mikami</b> and Akio Moriya</p><p><i>Mitoyo General Hostipal, Kanonji, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To clarify what specific lifestyle habits affect MASLD.</p><p><b><i>Subjects and Methods:</i></b> The subjects were 5866 participants who underwent health checkups including abdominal ultrasound examination between April 2014 and June 2022. We performed logistic regression analysis of the association with MASLD for exercise habits (>30 min), weight gain (more than 10 kg since age 20), high physical activity, walking speed, eating speed, dinner time, snack, skipping breakfast, alcohol consumption, and smoking.</p><p><b><i>Results:</i></b> The prevalence of MASLD in the subjects was 16.4%; the median BMI was 23.0, the median age was 54 years, and 55% were male. Overweight or obesity was present in 53%, glucose intolerance in 62%, hypertension in 41%, and dyslipidemia in 34%.</p><p>Results of logistic regression analysis adjusted for age, gender, and cardio metabolic risk factors showed that weight gain (odds ratio, 2.109; 95% confidence interval, 1.781-2.502) and high physical activity (0.830, 0.701-0.981) were the associated factors. When stratified by BMI, weight gain was an associated factor in normal weight, overweight, and obesity, excluding underweight. For normal weight only, high physical activity (odds ratio 0.668 for 95%, 0.450-0.978) and skipping breakfast (odds ratio 0.422 for 95%, 0.183-0.850) were also associated factors.</p><p><b><i>Conclusion:</i></b> Weight gain (more than 10 kg since age 20) was a particularly important factor associated with MASLD. High physical activity and skipping breakfast were also associated with MASLD only in the normal weight group.</p><p><b>PP-03-167</b></p><p><b>Beyond biopsy:Comparing Vibration-controlled transient elastography and 2D-shear wave elastography in Metabolic dysfunction-associated steatotic liver disease</b></p><p><b>S Sreekumar</b>, Arjun Haridas, Krishnadas Devadas and Srijaya Sreesh</p><p><i>Government Medical College, Thiruvananthapuram, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Vibration-controlled transient elastography(VCTE) and two-dimensional shear wave elastography(2D-SWE) are non-invasive techniques for liver stiffness measurement(LSM). We aimed to analyze the reproducibility of LSM on repeated VCTE and to compare LSM using 2D-SWE and VCTE in Metabolic dysfunction-associated steatotic liver disease(MASLD).</p><p><b><i>Materials and Methods:</i></b> 275 patients from MASLD clinic at Government Medical College, Thiruvananthapuram were included. LSM using VCTE with FibroTouch-FT100 were performed twice, 2 hours apart, in fasting patients. LSM using 2D-SWE with SuperSonic Imagine’s-Aixplorer was also performed on the same day.</p><p><b><i>Results:</i></b> The correlation coefficient between LSM on repeated VCTE was 0.96. The median LSM was 7.8(6.6-10.1)kPa and 8.3(6.8-10.4)kPa using VCTE and 2D-SWE respectively. The median difference in LSM between VCTE and 2D-SWE was 1.0kPa(0.5–1.5). In fibrosis ≤F2 and ≥F3, the difference was ±1.1kPa and ±0.9kPa respectively. Using Bland-Altman plot, significant disagreement between LSM values was noted in 21(7.63%). In multivariate analysis, central obesity and triceps skinfold thickness, were found to be independently associated with this. LSM values showed strong correlation(r=0.665). In fibrosis ≤F2 and ≥F3, correlation was 0.45 and 0.67 respectively. The area under the ROC curve for 2D-SWE in predicting ≥F3-fibrosis was 0.866. Based on Youden index, 2D-SWE cutoff of 9.9kPa had 79.5% sensitivity and 89.3% specificity in diagnosing ≥F3-fibrosis.</p><p><b><i>Conclusion:</i></b> Repeated LSM using VCTE has high degree of reproducibility. The difference in LSM between both techniques was more at lower values of LSM and showed stronger correlation in higher degrees of fibrosis. Given the strong diagnostic accuracy of 2D-SWE, both techniques could be used interchangeably.</p><p><b>PP-03-168</b></p><p><b>Fucoidan alleviated NASH via inhibiting oxidative stress by promoting GSTA2</b></p><p><b>Jingyi Si</b><sup>1</sup>, Yanting Zou<sup>1</sup>, Yifan Gao<sup>1</sup>, Xizhong Shen<sup>1</sup>, Changfeng Zhu<sup>1</sup> and Qunyan Yao<sup>1,2,3</sup></p><p><sup>1</sup><i>Zhongshan Hospital, Fudan University, Shanghai, China;</i> <sup>2</sup><i>Department of Gastroenterology and Hepatology, Zhongshan Hospital (Xiamen), Fudan University, Xiamen, China;</i> <sup>3</sup><i>Shanghai Geriatric Medical Center, Shanghai, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aims to explore the effect of fucoidan on non-alcoholic steatohepatitis (NASH) and the underlying molecular mechanisms by constructing a 3D NASH model based on Nucleic-Acid-nanostructures-decorated-living-Cells (NACs).</p><p><b><i>Materials and Methods:</i></b> This study constructed NAC NASH models from human cell lines and murine primary cells using NACs. Effects of fucoidan on lipid accumulation, fibrosis, and inflammation in NAC NASH models were investigated through pathological, biochemical detection and ELISA assay. RNA-seq technology was used to explore the underlying molecular mechanisms.</p><p><b><i>Results:</i></b> The pathological evaluation results showed that fucoidan could alleviate ballooning degeneration and cell necrosis in NAC NASH models, and could reduce lipid accumulation in liver cells and activation of stellate cell. Meanwhile, the immune assay for supernatant indicated that fucoidan could alleviate inflammation levels and collagen secretion in the NAC NASH models. The detection of reactive oxygen species (ROS), total superoxide dismutase (T-SOD) and malondialdehyde (MDA) indicated that fucoidan could reduce oxidative stress levels in the NAC NASH models. Meanwhile, transcriptome sequencing results indicated that fucoidan was involved in pathways such as TGF-β signaling pathway, inflammatory response, and reactive oxygen species metabolism. Further analysis indicated that GSTA2 was a key upregulated gene in the NAC NASH models treated with fucoidan.</p><p><b><i>Conclusion:</i></b> This study highlights the favorable properties of fucoidan for NASH, making fucoidan a promising candidate for NASH treatment.</p><p><b>PP-03-169</b></p><p><b>Vitamin E Improves Serum Markers and Histology in Adults with MASLD: Systematic Review and Meta-analysis</b></p><p>Nicholas Ming-Zher Chee, <b>Ram Prasad Sinnanaidu</b> and Wah Kheong Chan</p><p><i>University Of Malaya, Kuala Lumpur, Malaysia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and aim:</i></b> Multiple clinical trials have been conducted to study the potential benefits of vitamin E for the treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). This study aimed to assess the effect of vitamin E on serum markers of liver inflammation, specifically serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and histology, including resolution of metabolic dysfunction associated steatohepatitis (MASH), in adult patients with MASLD.</p><p><b><i>Methods:</i></b> A systematic literature search on randomised controlled trials published in English was conducted using electronic databases. Standardized mean difference (SMD) and mean difference (MD) were used for continuous outcomes, while risk ratio (RR) was used for dichotomous outcomes, with corresponding 95% confidence interval (CI).</p><p><b><i>Results:</i></b> A total of eight studies were included in the qualitative synthesis while seven studies were included in the meta-analysis. Vitamin E significantly reduced serum ALT and AST levels with SMD of -0.82 (95% CI, -1.13 to -0.51) and -0.68 (95% CI, -0.94 to -0.41), respectively. Vitamin E significantly reduced steatosis, lobular inflammation, and hepatocyte ballooning with a MD of -0.60 (95% CI, -0.83 to -0.37), -0.34 (95% CI, -0.53 to -0.16), -0.32 (95% CI, -0.53 to -0.12), and increased MASH resolution with a RR of 1.9 (95%CI, 1.20 to 3.02). However, vitamin E did not reduce fibrosis, with a MD of -0.23 (95% CI, -0.51 to 0.05).</p><p><b><i>Conclusion:</i></b> Vitamin E resulted in significant improvement in serum markers of liver inflammation and histology in patients with MASLD.</p><p><b>PP-03-170</b></p><p><b>Clinical Ultrasound Diagnosis of NAFLD and Complication of Metabolic Syndrome: Relationship with RTE, Attenuation Imaging</b></p><p><b>Masahiko Sugano</b></p><p><i>Sugano Internal Medicine Clinic, Himeji-City, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Aim</i></b>: NAFLD is the most abdominal ultrasonography examination cases in our clinic. Complicated fibrosis is important in relation to Metabolic Syndrome (MS). NAFLD has a high MS complication:20.7%. Among 1290NAFLD examples(2018-23), NAFLD Fibrosis Score (NFS): High(>0.676) MS merger is 40.4%, Intermediate: 24.4%, Low(<-1.455): 17.6%. Hepatic steatosis is also associated with MS, we examined the relation between ultrasonic fibrosis, steatosis evaluation and MS merger.</p><p><b><i>Matherial and Method</i></b>: We examined 1290 NAFLD cases (56.5y.o.; M725:F566, BMI:24.0, ALT27.0) who performed abdominal ultrasound in 2018-23 (March-May). Focusing on Strain Imaging; Real-time Tissue Elastography (Liver Fibrosis Index:LFI) and Share Wave Measurement (Vs) and Attenuation Imaging (ATT) at ultrasonic examination and Fib4index (F4i), M2BPGI (M2) were used to predict MS merger.</p><p><b><i>Correlation between each Fibrosis Index</i></b>: LFI between Vs(r=0.273)/ NFS(0.252)/ M2(0.179); LFI<1.58; Vs:1.17(mean)/ NFS:-2854/ M2:0.48, 1.58-2.03; 1.18/ -2.289/ 0.53, 2.03~2.40; 1.25/ -1.840/ 0.67, 2.40~2.86; 131/ -1.533/ 0.76, >2.86; 1.50/ -1.058/ 0.90. F4i between LFI (r=0.129)/ Vs(0.227)/ M2(0.590)/ NFS(0.724);Table.</p><p><b><i>Study of MS Merger rate</i></b>: LFI; <1.60 (56 cases): MS 1.8%, 1.60-1.80 (98):4.1%, 1.80-2.00 (170):4.1%, 2.00-2.20 (243):10.7%, 2.20-2.40 (267):16.9%, 2.40-2.60 (214):28.5%, 2.60-2.80 (129):43.4%, >2.80 (65):63.7%. MS cut off is considered as LFI>2.4 (sensitivity 0.69, specificity 0.74). The difference is not noticeable in Vs: (>1.25:MS25.5%; <1.25:16.1), F4i; (Low:22.5%, High:21.9). ATT:<0.62 (450):11.1%, 0.62-0.66 (184):17.9, 0.67-0.72 (190):24.2, >0.73 (206):34.5. MS C.O. is 0.67 (sensitivity 0.54, specificity 0.61).</p><p><b><i>Conlusion</i></b>: LFI can numerically estimate the MS merger of NAFLD (above 2.4). For serum markers NFS was useful. In NAFLD cases, MS should be searched for with a focus on LFI and reference to Vs, NFS, and ATT.</p><p><b>PP-03-171</b></p><p><b>Does name matter? unraveling the implications of nafld to mafld then masld</b></p><p><b>Erika Johanna Tañada-Escanlar</b> and Stephen Wong</p><p><i>University of Santo Tomas Hospital, Manila, Philippines</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The second-most recent change in steatosis nomenclature required 2/3 weight-metabolic criteria to be met and may lead to missed diagnosis in patients who develop weight-metabolic abnormalities later on. The metabolic-dysfunction-associated steatotic liver disease (MASLD) criteria requires only one criterion and may mitigate this. We aimed to characterize our steatotic patients into: 1.) nonalcoholic fatty liver disease (NAFLD) only; 2.) MASLD only; 3.) MAFLD only ; 4.) MAFLD-MASLD.</p><p><b><i>Materials and methods:</i></b> A cross-sectional analytical retrospective study reviewed 663 NAFLD patients with steatotic liver on ultrasound in a Hepatology clinic database from August 2007-July 2017 were included. Demographic, laboratories, AST/Platelet ratio (APRI), FIB4, and NAFLD fibrosis score (NFS) were compared between the groups.</p><p><b><i>Results:</i></b> The 663 steatotic patients were categorized into: 4(0.9%) NAFLD, 8 (1.2%) MASLD only, 325(49%) and 651(98.2% ) belongs to MAFLD-MASLD overlap. All MAFLD-MASLD diagnosed patients are diabetic while none of the MASLD and NAFLD groups are diabetic. More patients from the MASLD and NAFLD groups have hepatitis B than the MAFLD-MASLD group. On the other hand, more MAFLD-MASLD patients have hypertension (74.0%), dyslipidemia (83.1%), HCC (6.0%), cirrhosis (4.5%), and CAD/CVD (17.2%) than the other two groups. They all have high fibrosis risk with 14.6% likely to have advanced fibrosis.</p><p><b><i>Conclusions:</i></b> Majority (98.2%) of patients with steatosis on ultrasound meet MAFLD MASLD criteria which means that changing nomenclature has little difference on epidemiology. Additional longitudinal studies are needed to further illuminate on the implications of the terminology changes.</p><p><b>PP-03-172</b></p><p><b>Liver Stiffness in Metabolic-Associated Fatty Liver Disease With and Without Hepatitis: A Single Center Experience</b></p><p>Riki Tenggara<sup>1,3</sup>, Herlina Uinarni<sup>2,4</sup>, Mario Steffanus<sup>1,3</sup>, Titos Ahimsa<sup>3</sup>, Angelina Yuwono<sup>1</sup>, Sugento Sugento<sup>4</sup>, Denio Adrianus Ridjab<sup>5</sup>, <b>Alver Prasetya</b><sup>1</sup> and Rino Alvani Gani<sup>6</sup></p><p><sup>1</sup><i>Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia;</i> <sup>2</sup><i>Department of Anatomy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia;</i> <sup>3</sup><i>Department of Internal Medicine, Gastroenterology and Hepatology Division Pantai Indah Kapuk Hospital, Jakarta, Indonesia;</i> <sup>4</sup><i>Department of Radiology Pantai Indah Kapuk Hospital Jakarta Indonesia, North Jakarta, Indonesia;</i> <sup>5</sup><i>Department of Internal Medicine, Cardiology and Vascular Division Pantai Indah Kapuk Hospital, Jakarta, Indonesia;</i> <sup>6</sup><i>Division of Hepatobiliary, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Magnetic Resonance Elastography (MRE) is an advanced imaging technique used to assess fat accumulation and fibrosis in the liver, particularly in Metabolic-Associated Fatty Liver Disease (MAFLD). Hepatitis infection may contribute to the progression of liver fibrosis. This study aims to explore the correlation between liver stiffness in MAFLD patients with and without hepatitis infection.</p><p><b><i>Material and Methods:</i></b> This retrospective cross-sectional study included 46 patients with clinically diagnosed fatty liver who underwent MRE examination between 2021 and 2022. The study evaluated the correlation between baseline variables, including hepatitis infection, and liver stiffness.</p><p><b><i>Results:</i></b> Liver stiffness ranged from 1.45-7.80 kPa, with a geometric mean of 3.26 kPa and a coefficient of variation of 40.6%. No significant correlation was found between hepatitis infection and liver stiffness in MAFLD patients (p = 0.174). However, liver stiffness showed significant correlations with age (p = 0.047, r = 0.295), fibrosis markers (FIB-4 score, p < 0.001, r = 0.720; platelets, p < 0.001, r = -0.559), and indicators of hepatocellular damage (SGOT, p < 0.001, r = 0.615; GGT, p = 0.002, r = 0.533; ALP, p = 0.002, r = 0.573). Additionally, metabolic markers related to glucose control (HbA1c, p = 0.015, r = 0.501) and fat metabolism (total cholesterol levels, p = 0.011, r = -0.519) were correlated with liver stiffness.</p><p><b><i>Conclusion:</i></b> Hepatitis infection was not correlated with liver stiffness in MAFLD patients. In Indonesian fatty liver patients, liver stiffness was significantly correlated with age, liver fibrosis markers, hepatocellular damage, and metabolic dysfunction.</p><p><b>PP-03-173</b></p><p><b>Impact of Glucagon-like Peptide-1 Agonists on MASH/ MASLD: Meta-Analysis of 14 Studies</b></p><p><b>Daniel Yi Nin Tung</b><sup>1</sup>, Etham Tham<sup>2</sup>, Pojsakorn Danpanichkul<sup>3</sup>, Jieling Xiao<sup>2</sup> and Mark Muthiah<sup>1</sup></p><p><sup>1</sup><i>Division of Gastroenterology and Hepatology, Department of Medicine, National University Hospital, Singapore, Singapore;</i> <sup>2</sup><i>Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore;</i> <sup>3</sup><i>Department of Internal Medicine, Chiang Mai University, Chiang Mai, Thailand</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> This study aimed to evaluate the impact of GLP-1 agonists on patients with MASH/ MASLD via a systematic review and meta-analytic approach.</p><p><b><i>Materials and methods:</i></b> Medline and Embase were searched for studies which were selected based on evaluation of the effects of GLP-1 agonists on outcomes such as resolution of MASH, liver fat content, fibrosis-4 index, fibrosis, weight, transaminases, cholesterol and triglycerides. Data was analysed to obtain odds ratio or standardised mean difference (MD) comparing use of GLP-1 to patients without GLP-1 usage.</p><p><b><i>Results:</i></b> A total of 14 articles were included in the overall analysis. Patients treated with GLP-1 agonists had lower liver fat content (MD -3.82 CI -5.4 to -2.23; p<0.01), lower triglyceride levels (MD -39.68, CI -61.63 to -17.73; p<0.01), lower total cholesterol levels (MD -18.79, CI -35.19 to -2.39; p<0.01), lower LDL levels (MD -6.73, CI -11.45 to -2; p<0.01), lower HbA1c (MD -1.28, CI -1.66 to -0.91). They also had higher rates of resolution of MASH (OR 4.95 CI 3.6 to 6.82; p <0.01) and 1 or more point reduction in fibrosis with no worsening of MASH (OR 1.76, CI 1.29 to 2.42; p <0.01).</p><p><b>PP-03-174</b></p><p><b>Psyllium fiber improves hangovers and inflammatory liver injury by inhibiting intestinal drinking</b></p><p><b>Keungmo Yang</b><sup>1</sup>, Beom Sun Chung<sup>2</sup> and Tom Ryu<sup>3</sup></p><p><sup>1</sup><i>The Catholic University of Korea, Seoul, South Korea;</i> <sup>2</sup><i>Yonsei University Wonju College of Medicine, Wonju, South Korea;</i> <sup>3</sup><i>Soonchunhyang University College of Medicine, Seoul, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Excessive alcohol intake often results in hangovers and inflammatory liver damage, posing a significant health concern. Current treatment options for hangovers are still insufficient, highlighting the urgent need for new therapeutic approaches. Psyllium fiber (PF) is well-known for its gastrointestinal benefits, but its effect on hangovers is less explored. We utilized a mouse model with a single binge drinking (4 g/kg) to induce hangover and inflammatory liver injury. Intestine and liver injury were serologically and histologically estimated. Hangover symptoms were assessed using cylinder and footprint tests to objectively quantify hangover symptoms in mice. Binge drinking significantly activated alcohol-metabolizing enzymes in the small intestine and liver, leading to inflammatory damage. Concurrently, there was a rise in alcohol metabolites such as acetaldehyde and acetone, which exhibited a positive correlation with hangover symptoms in mice. Interestingly, the oral administration of PF (100 mg/kg) alongside alcohol consumption significantly reduced the activity of these enzymes and lowered the levels of alcohol metabolites. Mice treated with PF exhibited a considerable improvement in hangover symptoms and a reduction in hepatic inflammation, compared to control groups. Furthermore, in vitro experiments using HepG2 cell lines and semipermeable membranes demonstrated that PF effectively inhibits alcohol absorption into the body. In conclusion, PF demonstrates a potential protective effect against alcohol-induced hangover and liver injury by inhibiting the absorption of alcohol and lowering hangover-related alcohol metabolites. This study suggests that PF could serve as an effective therapeutic option for mitigating the adverse effects of excessive alcohol consumption.</p><p><b>PP-03-175</b></p><p><b>The prognostic impact of psychiatric intervention on alcohol-associated liver disease: the UK Biobank cohort study</b></p><p><b>Keungmo Yang</b><sup>1</sup>, Sunghwan Kim<sup>1</sup>, Hyun Yang<sup>1</sup>, Bumseok Jeong<sup>2</sup>, Hyun Kook Lim<sup>1</sup> and Si Hyun Bae<sup>1</sup></p><p><sup>1</sup><i>The Catholic University of Korea, Seoul, South Korea;</i> <sup>2</sup><i>Korea Advanced Institute for Science and Technology, Daejeon, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background/Aims:</i></b> Alcohol-associated liver disease (ALD) is a public health concern. ALD patients often have psychiatric comorbidities, but the effects of psychiatric interventions on ALD are not well-established. This study explores the prognostic impact of psychiatric intervention on ALD within UK Biobank cohort.</p><p><b><i>Methods:</i></b> This prospective study included a total of 502,370 participants. Psychiatric intervention was defined by a consultation with psychiatrists during hospitalization or a history of medication related to alcohol use disorder and psychiatric comorbidities. Survival analysis was conducted, incorporating propensity score matching (PSM) and doubly robust (DR) estimation, to precisely assess the impact of psychiatric intervention.</p><p><b><i>Results:</i></b> Among 2,417 ALD patients in the final analysis, those with F10 codes had poorer survival outcomes. Psychiatric intervention significantly improved the outcomes of both all-cause and liver-related mortality and reduced the incidence of liver cirrhosis. In subgroup analyses or 2-year landmark analyses, psychiatric intervention consistently showed a survival benefit in ALD patients. In the multivariate analysis, psychiatric intervention was identified as a favorable prognostic factor (hazard ratio, 0.780; P = 0.002 after PSM). Furthermore, the average treatment effects from DR estimation provided detailed insights into the impact of psychiatric intervention (2.9 years, P < 0.001 after PSM).</p><p><b><i>Conclusions:</i></b> This study demonstrates the favorable effect of psychiatric intervention in ALD patients with psychiatric comorbidities. These findings emphasize the importance of integrated management for ALD patients to address both their medical and psychiatric aspects. Therefore, we suggest the potential benefits of early psychiatric interventions in improving survival outcomes in ALD.</p><p><b>PP-03-176</b></p><p><b>Frailty, Genetic Susceptibility and Non-alcoholic Fatty Liver Disease: from Two Large Cohorts and Genetic Analyses</b></p><p><b>Han Zhang</b><sup>1</sup>, Lingyi Li<sup>1</sup>, Lijun Zhang<sup>1</sup>, Zheng Li<sup>2</sup>, Yuying Ma<sup>1</sup>, Wentao Huang<sup>1</sup>, Ruijie Zeng<sup>1</sup>, Dongling Luo<sup>1</sup>, Yanjun Wu<sup>1</sup>, Meijun Meng<sup>1</sup>, Felix W Leung<sup>3</sup>, Chongyang Duan<sup>4</sup>, Weihong Sha<sup>1</sup> and Hao Chen<sup>1</sup></p><p><sup>1</sup><i>Guangdong Provincial People's Hospital, Guangzhou, Guangdong Province, China;</i> <sup>2</sup><i>Shantou University Medical College, Shantou, Guangdong Province, China;</i> <sup>3</sup><i>University of California Los Angeles, Los Angeles, United States of America;</i> <sup>4</sup><i>Southern Medical University, Guangzhou, Guangdong Province, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To evaluate the association, causality and genetic correlation between frailty and non-alcoholic fatty liver disease (NAFLD).</p><p><b><i>Materials and Methods:</i></b> UK Biobank and the National Health and Nutrition Examination Survey (NHANES) respectively employed Cox proportional hazard regression models and logistic regression models to explore the role of frailty in NAFLD. Frailty Index (FI) and Frailty Phenotype (FP) were used to assess the frailty status. The combined effects of genetic susceptibility were explored using the polygenic risk score (PRS). Mendelian Randomization, Linkage Disequilibrium Score Regression (LDSC), Genetic Covariance Analyzer (GNOVA) and Heritability Estimation from Summary Statistics (ρ-HESS) were used to explore the causal association and genetic correlation.</p><p><b><i>Results:</i></b> In the FP analysis using UK biobank, pre-frail (hazard ratio [HR] 1.48; 95% confidence intervals [CI] 1.38-1.58; P<0.001) and frail individuals (HR 2.10; [95% CI 1.92-2.30]; P<0.001) had increased risks of NAFLD. In the FI, the risk of NAFLD increased in individuals under pre-frailty (HR 1.93; [95% CI 1.80-2.06]; P<0.001) and frailty (HR 3.50; [95% CI 3.24-3.77]; P<0.001). Similar results were obtained in the NHANES. Subgroup analysis based on PRS showed even pre-frail/frail people with low genetic susceptibility were also at risk for NAFLD. There was significant causality (OR 2.00; [95% CI 1.40-2.86]; P<0.001) and positive genetic correlations (LDSC: rg =0.576, P<0.001; GNOVA: rg =0.777, P<0.001; ρ-HESS: rg =0.828) between FP and NAFLD.</p><p><b><i>Conclusion:</i></b> Frailty greatly increases the risk of developing NAFLD, even in individuals with lower genetic risk. There was a significant causal and positive genetic correlation between FP and NAFLD.</p><p><b>PP-03-177</b></p><p><b>The association between triglyceride-glucose index and its combination with obesity indicators and MAFLD: NHANES 2017-2020</b></p><p>Jiayin Yu<sup>2</sup>, Yutong Bai<sup>2</sup>, Bo Wang<sup>1</sup>, Yongqiang Xiong<sup>1</sup> and <b>Shu Zhang</b><sup>1</sup></p><p><sup>1</sup><i>The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;</i> <sup>2</sup><i>Xi’an Jiaotong University, Xi’an, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> The relationship between the triglyceride-glucose (TyG) index and TyG combined with indicators of obesity and MAFLD has been less well defined.</p><p><b><i>Methods:</i></b> This cross-sectional study included 2007 adults from the National Health and Nutrition Examination Survey (NHANES) 2017-2020.Liner regression, binary logistic regression analyses, and receiver operating characteristic (ROC) were used to analyze the relationship between TyG and its combined obesity-related indicators and MAFLD.</p><p><b><i>Results:</i></b> TyG, WHtR, and TyG-WHtR were significantly and positively associated with controlled attenuation parameter and MAFLD (TyG: OR 4.269, 95%CI 3.594-5.071, WHtR: OR 3.256, 95%CI 2.871-3.692, TyG-WHtR: OR 3.843, 95%CI 3.372-4.379). ROC curves showed that these three indicators were all related to MAFLD, TyG-WHtR had more robust diagnostic efficacy than TyG and WHtR (95%CI 0.795-0.832).</p><p><b><i>Conclusions:</i></b> TyG and TyG-WtHR enhance diagnostic efficacy of MAFLD. TyG-WtHR are expected to become more effective metrics for identifying populations at early risk of MAFLD.</p><p><b>PP-03-178</b></p><p><b>WY14643, a agonist of PPARα, attenuates HFD-fed AIH via inhibiting JNK signaling pathway</b></p><p><b>Cuijuan Zhao</b> and Lu Zhou</p><p><i>Department Of Gastroenterology And Hepatology, Tianjin Medical University General Hospital, Tianjin, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> The regulation molecular mechanism of HFD on the occurrence and development of AIH by PPAR α and JNK signaling pathway.</p><p><b><i>Materials and methods:</i></b> Mice were fed for 16 weeks either normal chow diet (ND) or high fat diet (HFD). Mice were intravenously administered Con A (20 mg/kg) to induce AIH. GW6471 (20 mg/kg) or WY14643 (6 mg/kg) was injected intraperitoneally for 3days. The mice and liver and spleen tissues were weighted. The histopathological analysis was performed by HE staining.The mRNA and protein levels were detected using RT-qPCR and Western blotting.</p><p><b><i>Results:</i></b> HFD could exacerbate the ConA-induced AIH, including liver steatosis and inflammation. Compared with other groups, necroptosis and apoptosis-related proteins, which were expressed at abnormally high levels in liver tissues of HFD+ConA group, were significantly up-regulated by HFD treatment. Therefore, HFD could promote the necroptosis and apoptosis in AIH mice. Meanwhile, HFD could inhibit the expression of PPARα and activate the JNK signaling pathway in AIH mice. Furthermore, PPARα inhibitor GW6471 could further aggravate the necroptosis and apoptosis of AIH mice and PPARα agonist WY14643 could alleviate the deterioration of HFD in AIH mice. Importantly, PPARα agonist WY14643 could further alleviate the necroptosis and apoptosis of AIH mice through inhibiting the JNK signaling pathway.</p><p><b><i>Conclusion:</i></b> PPARα could have efficacy on inhibiting HFD-fed AIH progression by inhibiting the necroptosis and apoptosis of hepatocyte and the lipid metabolism abnormity of liver. Further molecular mechanism study found that WY14643 could upregulate the PPARα and inhibit the function of activating JNK signaling pathway.</p><p><b>PP-03-179</b></p><p><b>Estimation of fibrosis in autoimmune hepatitis with Shear wave Elastography</b></p><p>Raja Taha Yaseen Khan, <b>Vijesh Kumar</b>, Abbas Ali Tasneem and Nasir Hassan Luck</p><p><i>Sindh Institute Of Urology And Transplantation, Karachi, Pakistan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> This study aimed to determine the association between histologic liver fibrosis and Shear Wave Elastography (SWE) measurements of liver stiffness in patients with autoimmune hepatitis (AIH).</p><p><b><i>Material and Methods:</i></b> Conducted at the Hepatogastroenterology unit at Sindh Institute of Urology and Transplantation Karachi, this cross-sectional study included all diagnosed AIH patients who had received a liver biopsy and undergone SWE from March 2022 to December 2023. Patients with other liver diseases were excluded. Liver fibrosis was evaluated using the Metavir scoring system. SWE measurements, performed by an experienced radiologist, required patients to fast for at least 4 hours before the examination. SWE results were compared with histologic findings from liver biopsies. Data were analyzed using SPSS 23.0.</p><p><b><i>Results:</i></b> The study included 162 patients (91 males, 71 females) with a mean age of 35.8 ± 16.6 years. ANA was positive in 123 patients, ASMA in 47, and anti-LKM in 4. SWE detected fibrosis stages as F1 in 50 (30.9%), F2 in 44 (27.1%), F3 in 40 (24.7%), and F4 in 28 (17.3%) patients. Liver biopsies revealed severe fibrosis in 40 patients and cirrhosis in 30. SWE measurements significantly correlated with Metavir scores from liver biopsies (p < 0.001), with higher fibrosis stages (F3-F4) showing higher SWE measurements.</p><p><b><i>Conclusion:</i></b> SWE is a reliable non-invasive method for estimating liver fibrosis in AIH patients, significantly correlating with histologic findings. This technique can potentially reduce the need for liver biopsies, minimizing associated risks and improving patient care.</p><p><b>PP-03-180</b></p><p><b>A Case of Sustained Virological Response of Chronic Hepatitis E With Ribavirin and Zinc Therapy</b></p><p><b>Huanghuan Li</b><sup>1</sup>, Yiying Pei<sup>2</sup> and Rajneesh Kumar<sup>2</sup></p><p><sup>1</sup><i>Sengkang General Hospital, Singapore, Singapore;</i> <sup>2</sup><i>Singapore General Hospital, Singapore, Singapore</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Hepatitis E virus (HEV) is a one of the leading causes of hepatitis worldwide. Here, we report a case of refractory chronic hepatitis E achieving sustained virological suppression with 2 months of dual therapy of ribavirin and zinc.</p><p>72 year old Chinese female was seen in 2016 for AST of 325 U/L and ALT of 225 U/L. She has background of Evan Syndrome with immune thrombocytopenia (ITP) and Systemic Lupus Erythematous (SLE) on high dose prednisolone since 2012. Attempt was made to reduce her prednisolone dose, followed by ribavirin 400mg daily for 3 months in view of persistence of HEV viral load. Undetectable HEV and normal transaminases were noted thereafter. Seven months after stopping ribavirin, AST and ALT rose to 159 IU/ml and 129 IU/ml with detectable HEV. He was restarted on ribavirin. HEV RNA persisted around 3.4 – 7 log for the next 6 years. Fibroscan showed stage 1 fibrosis in 2022 and 2023. Zinc 50mg daily was added in Nov 2023 after 6 years of maintenance ribavirin therapy. HEV RNA was undetectable in PCR since January 2024 with normal transaminases.</p><p>There are currently no established treatment options for chronic HEV patients who could not achieve sustained virological response in spite of reduction in immunosuppressant and ribavirin use. In our case report, zinc 50mg OM was added to ribavirin therapy for 2 months. Chronic HEV was suppressed. This is one of the first few case reports on use of zinc and ribavirin dual therapy in achieving chronic HEV suppression.</p><p><b>PP-03-181</b></p><p><b>Performance of noninvasive seromarkers in predicting liver fibrosis in mafld patients</b></p><p><b>Annisa Zahra Mufida</b><sup>1</sup>, Nurike Setiyari Mudjari<sup>2</sup> and Titong Sugihartono<sup>1</sup></p><p><sup>1</sup><i>Medical Faculty, Airlangga University, Surabaya, Indonesia;</i> <sup>2</sup><i>Dr Soetomo Teaching Hospital, Surabaya, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objective:</i></b> To compare Fibrosis-4 (FIB-4), AST to platelets ratio index (APRI) and NAFLD fibrosis score (NFS) to FibroScan for the assessment of hepatic fibrosis in MAFLD patients.</p><p><b><i>Material and Method:</i></b> A cross-sectional study was conducted on adult T2DM patients at Dr. Soetomo Hospital, Surabaya, Indonesia. Subjects underwent laboratory and transient elastography (TE) examination. The liver stiffness measurement (LSM) and controlled attenuation parameter (CAP) determined using FibroScan (Echosense). Patient with history of alcohol, hepatitis B or C, autoimmune hepatitis, and steatogenic drugs were excluded. The variables include the APRI Score, FIB-4, NFS, and FibroScan Score. Analysis was conducted using Pearson correlation, independent t test, and ROC curve analysis.</p><p><b><i>Results:</i></b> There were 80 patients in this study with an average age of 54.83 years and female patients (55.0%). Independent test results showed that there were significant differences in APRI Score, FIB-4, and NFS between patients who did not experience fibrosis (F0 and F1) and patients who experienced fibrosis (F2, F3, and F4). The results showed that NFS could diagnose liver fibrosis (F2, F3, and F4) compared to APRI Score and FIB-4 with AUC 0.693, optimal cut off -1.265, sensitivity 95% and specificity 85%. The APRI Score index obtained an AUC of 0.685, optimal cut off 0.155, sensitivity 80%, and specificity 48.3%. Meanwhile, the FIB-4 index obtained an AUC of 0.693, an optimal cutoff of 0.655, a sensitivity of 95% and a specificity of 85%.</p><p><b><i>Conclusion:</i></b> NFS is the best index for diagnosing liver fibrosis compared to APRI Score and FIB-4.</p><p><b>PP-03-182</b></p><p><b>Risk factors for primary biliary cholangitis combined with Sjogren's syndrome: a cohort of patients from China</b></p><p><b>Yu Peng</b> and Xiaowei Liu</p><p><i>Xiangya Hospital, Central South University, Changsha, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> To know about the risk factors of primary biliary cholangitis (PBC) combined with Sjogren's syndrome in the Chinese patients.</p><p><b><i>Materials and Methods:</i></b> Retrospective analysis of data from resident patients diagnosed with PBC at Xiangya Hospital from January 2017 to December 2023 was performed. The patients were divided into groups combined with or without Sjogren's syndrome. A total of 42 items from clinical features to laboratory tests were included in the statistical analysis. Data were analyzed using Chi-squared test, Fisher exact test, Logistic regression.</p><p><b><i>Results:</i></b> 93 consenting PBC combined with Sjogren's syndrome patients, were compared with 93 PBC patients matched for age. Among the patients, 21(11.3%) male and 165(88.7%) female. Chi-squared test showed that PBC combined with Sjogren's syndrome was related to the following factors: 1) gender (p<0.05); 2) the level of extractable nuclear antigen (ENA) (p<0.001); 3)c. the level of total bile acid (TBA) (p<0.05); 4) the level of prothrombin time (PT) (p<0.05); 5) the level of international normalized ratio (INR) (p<0.005); 6) the level of activated partial thromboplastin time (APPT) (p<0.05). The independent risk factors for PBC combined with Sjogren's syndrome was the level of INR (odds ratio (OR): 5.683, 95% confidence interval (CI): 1.401-31.719, p=0.017).</p><p><b><i>Conclusion:</i></b> This pilot study suggests that the independent risk factors for PBC combined with Sjogren's syndrome in Chinese patients was the level of INR. Indeed, the pathogenic mechanisms, clinical features, and optimal therapeutic approaches for PBC and Sjogren's syndrome are not yet fully defined. This issue should be systematically investigated.</p><p><b>PP-03-183</b></p><p><b>Unveiling the Enigma, case of Mosaic Alpers Syndrome</b></p><p><b>Vujwal Roy</b></p><p><i>Aster Medcity, Kochi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Alpers-Huttenlocher syndrome, first received attention from Alpers in 1931 and was further described with its hepatic manifestation by Huttenlocher et al. It is a rare mitochondrial disease characterized by its classic triad of refractory seizures, psychomotor regression, and hepatopathy.</p><p><b><i>Case discussion:</i></b> 38 years male, known Decompensated Cirrhosis of Liver presented to our institute with c/o progressive abdominal distension. Ascitic Fluid study was suggestive of Spontaneous Bacterial peritonitis and was started on Antibiotics and other supportive measures.</p><p>His past history revealed history of refractory seizures, hemineglect, hemianopia, Sensorineural hearing loss and hemiparesis at the age of 21 years, for which he was started on dual anticonvulsants elsewhere. MRI Brain showed hyperintense gyri suggestive of Mitochondrial disease. CSF Analysis showed lymphocytic pleocytosis with high protein, viral etiologies were ruled out. He also had high lactate levels and a diagnosis of MELAS was made and he was started on oral steroids. He persisted to have intermittent episodes of seizures for which another anticonvulsant was added. He developed pedal edema and was diagnosed to have cirrhosis at 31 years of age.</p><p><b><i>Discussion:</i></b> In current admission, considering his liver disease, encephalopathy, seizures a clinical diagnosis of Mosaic Alper Syndrome was made. He has been counselled for liver transplantation and is currently awaiting donor. Alpers-Huttenlocher syndrome is a mitochondrial disorder polymerase gamma(POLG) mutations, that decrease mitochondrial DNA replication. Ultimately, POLG dysfunction induces a progressive depletion of mitochondrial DNA and evolving organ dysfunction. Mosaic Alpers is a milder form or variant of classic Alpers syndrome presentation</p><p><b>PP-03-184</b></p><p><b>Pyogenic hepar abscess et causa mtb and salmonella paratyphi serotype a: a case report</b></p><p><b>Arlyando Saragih</b></p><p><i>Hermina Jatinegara, Jakarta Timur, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> A liver abscess is defined as a pus-filled mass in the liver. The incidence of liver abscess is typically low, the annual incidence rate is about 2.3 cases per 100,000 people. It is essential to early detect and manage these lesions, since there is a significant mortality risk in untreated patients. One of the most common forms of liver abscess is pyogenic liver abscess.</p><p><b><i>Case Description:</i></b> We report a case of liver abscess that occurred in segment IV of the liver, with non-specific complaints, with culture results showing 2 causative germs, namely Mycobacterium Tuberculosis and Salmonella Paratyphi Serotype A. The patient came with chief complaints of middle abdominal pain accompanied by nausea and intermittent fever. Radiological examination in the form of CT-Scan and USG confirmed the diagnosis of liver abscess in the fourth segment. Aspiration abscess drainage combined with antibiotic therapy and anti tuberculosis is a safe and effective therapeutic approach for this case.</p><p><b><i>Discussion:</i></b> Liver abscess has a low incidence rate, and not a very common case. This case shows that suspicion is necessary for the diagnosis of liver abscess in a young woman with good socio-economic and nutritional conditions, and the importance of carrying out a complete examination of the specimen to determine the exact etiology.</p><p><b>PP-03-185</b></p><p><b>Clinical presentation and outcome of Hepatic Langerhans cell histiocytosis: Two-decade experience from a tertiary center</b></p><p><b>Jayendra Seetharaman</b>, Arul Premanand Lionel, Leni Grace Mathew, Mercy Pulipatti and Leenath Thomas</p><p><i>Christian Medical College, Vellore, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Langerhans cell Histiocytosis (LCH) is a rare disorder of children characterised by neoplastic dissemination of CD1a and CD207 (langerin) positive cells. The liver involvement of LCH happens in isolation or as a part of multisystemic disease. The literature on clinical presentations and outcome following treatment are scarce. The study aims to assess the presentation of hepatic LCH and liver outcomes following treatment in children</p><p><b><i>Methods:</i></b> Children < 18 years, diagnosed LCH between 2003-2023 with any one a. Presence of hepatomegaly/ Jaundice/ ascites b. deranged liver function tests (LFT) c. abdominal imaging suggesting liver involvement were included. The presentations and outcomes following treatment were retrospectively analysed.</p><p><b><i>Results:</i></b> 66 children [mean age (21.83 ± 12.63) months, 48 boys] diagnosed hepatic LCH were included. The duration of symptoms was 5.174 ± 3.75 months. Most common presentations were fever (93.9%) followed by abdominal distention (80.3%), anemia (75.8%) and jaundice (39.4%). 65 (98.4%) underwent ultrasonogram (USG) of which 22 (33.3%) underwent computed tomography (CT) abdomen and 10 underwent liver biopsies. 5 (7.6%) had isolated liver involvement. 56 children had at least one follow-up (30.09 ± 32.53 months). 20 were cured, 30 has persistent LCH, 10 died either on or after treatment. 31 (55.3%) children had liver dysfunction (jaundice, portal hypertension, sclerosing cholangitis) during last follow-up. Characteristics of children with good and bad liver outcomes were compared (Table 1).</p><p><b><i>Conclusion:</i></b> Children with hepatic LCH has high mortality and morbidity. Presence of jaundice, anaemia, elevated transaminases and abnormal parenchymal architecture at presentation predicts poor liver outcome.</p><p><b>PP-03-186</b></p><p><b>Unique case of Budd Chiari Syndrome with Sagittal sinus Thrombosis By Dr Zafar</b></p><p><b>Athesham Zafar</b></p><p><i>Walsall Manor Hospital, Walsall, United Kingdom</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Budd-Chiari syndrome (BCS) is a rare but serious condition characterized by the obstruction of hepatic venous outflow, leading to liver damage. This blockage can result from thrombosis, compression, or other forms of obstruction in the hepatic veins or the inferior vena cava. Early diagnosis and treatment are crucial.</p><p><b><i>Case Description:</i></b> 28 year old female who attended her doctor due to 2 months of lethargy and abdominal pain. An ultrasound was performed that showed gross ascites. (CT) was arranged that showed a heterogenous liver parenchyma with faintly visible hepatic veins. It therefore was decided to transfer her to our liver unit at Birmingham.</p><p>She also complained of headache and blurring of her vision. Fundoscopy was performed it showed papilledema.</p><p>CT triple phase showed thready left hepatic artery and collapsed hepatic veins. Histology from liver biopsy found severe venous outflow obstruction with confluent perivenular necrosis, in keeping with the diagnosis of Budd Chiari Syndrome.</p><p>CT Venogram Confirmed Sagittal Sinus Thrombosis. She was prothrombotic.</p><p>Accordingly, the patient was started on low molecular weight (LMW) heparin and underwent a Transjugular intrahepatic portosystemic shunt (TIPPS) without complication.</p><p>The cause of her prothrombotic state remained uncertain and she lacked obvious risk factors. Flow cytometry identified a substantial clone of Paroxysmal Nocturnal Haemoglobinuria. She was started on Eculizumab for treatment of PNH.</p><p><b><i>Discussion:</i></b> Budd-Chiari syndrome requires a high index of suspicion for early diagnosis. Management strategies for BCS include anticoagulation and Liver transplantation may be considered in cases of severe liver failure.</p><p><b>PP-03-187</b></p><p><b>Littoral Cell Angioma as a Cause of Non Cirrhotic Portal Hypertension with Severe Splenomegaly</b></p><p><b>Donald Boy P Purba</b>, Budi Widodo and Titong Sugihartono</p><p><i>Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Dr. Soetomo General Academic Hospital, Universitas Airlangga, Surabaya, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Littoral Cell Angioma (LCA) is a rare primary tumor of the spleen arises from the lining cells of the trabeculated mesh in the reticuloendothelial system. Only 150 cases reported in the medical literature, but none of them from Indonesia.</p><p><b><i>Case Illustration:</i></b> A 28year old male presented with a three month history of upper left abdominal pain, abdominal distension, nausea, decreased appetite, jaundice, and dark urine. Physical examination revealed jaundice, hepatosplenomegaly, and ascites. Laboratory tests showed leukocytosis, thrombocytosis, direct hyperbilirubinemia, and elevated transaminases. Abdominal ultrasound revealed hepatosplenomegaly, ascites, and multiple nodules on the spleen. Contrast enhanced CT scan showed hepatomegaly without cirrhosis signs, splenomegaly, ascites, and perisplenic sub lymph paraaortic portocaval lymphadenopathy. Endoscopy revealed grade III esophageal varices with bleeding signs, antral varices, moderate portal hypertension gastroduodenopathy confirming noncirrhotic portal hypertension. Spleen FNAB showed CD3 positive endothelial cells lining capillary blood vessels, with CD30 and CD34 negativity, consistent LCA. The patient was treated furosemide 40 mg, propranolol 40 mg, spironolactone 100 mg daily. splenorenal shunt surgery was performed to reduce portal pressure.</p><p><b><i>Discussion:</i></b> LCA can occur across various age groups and genders. Clinical manifestations include splenomegaly of unknown etiology, accompanied by abdominal pain, sometimes incidentally discovered as single or multiple lesions on the spleen. Characteristic findings on CT, MRI, ultrasound, and Tc99m RBC scan correlate with histopathological and immunohistochemical features. Definitive diagnosis of LCA relies on morphological and immunohistochemical features distinguishing it from other vascular lesions of the spleen.</p><p><b>PP-03-188</b></p><p><b>Updated Burden of Stomach Cancer in Hong Kong Chinese: a time-trend analysis</b></p><p><b>Junjie Huang</b>, Yat Ching Fung, Chenwen Zhong, Sze Chai Chan and Martin Wong</p><p><i>The Chinese University Of Hong Kong, Sha Tin, Hong Kong</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of stomach cancer.</p><p><b><i>Methodology:</i></b> Data on new cases and deaths related to stomach cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).</p><p><b><i>Results:</i></b> In 2020, there were 1,197 newly reported stomach cancer cases in Hong Kong, with an ASR of 6.9. The incidence was higher among males (ASR=8.7) compared to females (ASR=5.3), and remarkably higher in the older population aged over 50 years (ASR=29.3) compared to the younger population (ASR=1.3). There were 613 newly reported deaths, with an ASR of 3.2. Similarly, the mortality was higher in males (ASR=4.1) than in females (ASR=2.4), and substantially higher in the older population (ASR=13.8) compared to the younger population (ASR=0.4). Trend analysis revealed a decreasing mortality trend for both sexes and age groups, while no significant increase or decrease was observed in the overall incidence trend. Notably, the male population was the only group with a significant decline in both incidence (AAPC: -1.5, 95% CI: -2.3, -0.6, p=0.005) and mortality (AAPC: -3.4, 95% CI: -5.8, -1.0, p=0.006) trends.</p><p><b><i>Conclusion:</i></b> The findings of this population-based study suggest that while the mortality of stomach cancer in Hong Kong is decreasing, the incidence remains relatively stable. Continued efforts to promote healthy dietary habits could be crucial in lowering the overall incidence of stomach cancer.</p><p><b>PP-03-189</b></p><p><b>Endoscopic Ultrasonographic Diagnosis of Pancreatic Tuberculosis: A Case Report</b></p><p><b>Sri Angraeni</b>, Saskia Aziza Nusyirwan and Hasan Maulahela</p><p><i>Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Departement of Internal Medicine, Faculty of Medicine, University of Indonesia, Ciptomangunkusumo Hospital, Jakarta, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Despite the high prevalence of tuberculosis (TB) in developing countries, pancreatic TB remains a rare disease. Endoscopic ultrasound provides high resolution images of the pancreatic lesions as well as an opportunity to sample these lesions for cytological confirmation.</p><p><b><i>Case Presentation:</i></b> A 23-year-old female patient came with chief complaint abdominal pain since 3 month ago, weight lost 4 kg in the last 3 months, and there was lump in the left side of the neck. Physical examination showed abdominal pain and left colli lymphadenopathy, HIV test was negative, chest x ray was normal. MRCP showed multiple loculated/ lobulated cysts in caput, uncinate and proximal of pancreatic body and multiple lymphadenopathy at para-aorta. Endoscopic ultrasound with fine-needle aspiration (EUS FNA) was performed and found peri-pancreatic lymphadenopathy, hypoechoic, with the clear boundary. We performed cytology and TB PCR test. The result of TB PCR was positive. Cytology examination from EUS FNA found granulomatous chronic inflammation, and cytology of left colli lymphadenopathy showed chronic lymphadenitis were suspected caused by tuberculosis.</p><p>The final diagnosis were pancreatic tuberculosis and tuberculous lymphadenitis. The patient is currently undergoing the 11th months out of the planned 12 months of standard treatment of anti tubercular therapy. Now the patient no longer experiences complaints of abdominal pain and lump in the neck, her weight has increased.</p><p><b><i>Discussion:</i></b> Pancreatic tuberculosis is one of the rarest forms of tuberculosis and it presents with non- specific clinical manifestations. Regarding establishment of an accurate diagnosis, endoscopic ultrasound with fine-needle aspiration has an important role.</p><p><b>PP-03-194</b></p><p><b>Ulcerative Collitis with Colorectal Carcinoma</b></p><p><b>Andry Kurniawan</b>, Lily Fajriati, Vesri Yoga, Saptino Miro and Arnelis Arnelis</p><p><i>Rumah Sakit Umum Pusat Dr. M. Djamil Padang, Padang, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Ulcerative colitis with colorectal cancer has presented a series of clinical problems, with its poorer prognosis and higher mortality rate than sporadic colorectal carcinoma. Chronic inflammation is a significant contributor to the development of ulcerative colitis with colorectal carcinoma. In the management of ulcerative colitis, it can depend on the severity of the disease.</p><p><b><i>Case description:</i></b> 62 year old female patient with bloody stools that had increased since 2 days ago. Diarrhea had occured since 6 months ago. Based on physical examination, BP was found to be 140/90 mmHg, anemic conjunctiva and epigastric pain. The results of supporting examinations showed that Hb was 9.3 gr/dl, a chest x-ray examination revealed cardiomegaly with a CRT > 60%. Colonoscopy showed severe ulcerative colitis with descending colon Ca, the examination was then followed by colon biopsy with results of colon carcinoma with ulcerative colitis. The patient received Mesalazine therapy 3x500 mg with prednisone 3x 10 mg. The patient also received a 1 unit PRC transfusion while the bleeding was still active. During treatment, the patient showed clinical improvement as indicated by the cessation of rectal bleeding and reduced frequency of diarrhea.</p><p><b><i>Discussion:</i></b> Patients with ulcerative colitis have a two- to three-fold increased risk of developing colorectal cancer compared to the general population. Ulcerative colitis with colorectal cancer has poorer prognosis and higher mortality rate than sporadic colon carcinoma.</p><p><b><i>Key words:</i></b> ulcerative colitis, Colorectal Carcinoma</p><p><b>PP-03-257</b></p><p><b>Celiac disease: variable presentations & social implications, data from a developing country</b></p><p><b>Talal Bhatti</b> and Madeha Irfan and Fareed Muslim and Ali Sufyan</p><p><i>Szabmu, Islamabad, Pakistan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Intro:</i></b> Celiac disease is an autoimmune enteropathy triggered by ingestion of gluten in genetically susceptible individuals. It has varied clinical presentations. Not much is known about presentation of this disease in Pakistani adults.</p><p><b><i>Methods:</i></b> This is a cross sectional study conducted at a public sector tertiary care setup of Islamabad. Patients who were diagnosed cases of Celiac disease on the basis of serology and/or histopathology were interviewed via a structured questionnaire via non probability consecutive sampling technique. statistical analysis was performed IBM SPSS version 25.</p><p><b><i>Results:</i></b> A total of 340 patients were enrolled in present study. Most predominant intestinal symptom was Diarrhea reported by 46.8% of participants followed by Abdominal pain/Bloating in 44.7% (Figure I). Most common Extra-intestinal features were Anemia reported by 51.5% of patients (Figure II). Of note constipation was reported by 21.5% and Stunted growth by 35.3% of patients. Most common utilized diagnostic modality was Serology in 62.9% of patients (Figure III). 86.8% of patients reported symptom resolution with Gluten free diet. This association was statistically significant. Patients who reported Abdominal pain/Bloating were significantly more likely to report improvement in symptoms following Gluten free diet (Figure IV). Challenges in Gluten free diet were availability issues reported by 37.5% followed by financial issues in 25%, social challenges in 19.2% and other issues including compliance and cross contamination by 40% (Figure V)</p><p><b><i>Conclusion:</i></b> Celiac Disease has an extremely Variable Presentation in Pakistani patients. It can present with Classical Diarrhea and Non classical presentation with abdominal pain and bloating as well.</p><p><b>PP-03-258</b></p><p><b>Celiac disease- extra intestinal presentations; perspective from a developing country</b></p><p><b>Talal Bhatti</b> and Fareed Muslim and Madeha Irfan and Ali Sufyan</p><p><i>Szabmu, Islamabad, Pakistan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Celiac disease (CD) is an immune mediated bowel disorder characterized by sensitivity to gluten. Diagnosis of celiac disease is made by history and clinical presentation compatible with CD, serological screening compatible with CD, histological findings, compatible with CD, obvious clinical and serological response to a gluten-free diet. Extra Intestinal manifestations in Celiac population in Pakistan in not well studied.</p><p><b><i>Methods:</i></b> This is a cross sectional study conducted at a public sector tertiary care setup of Islamabad. Patients who were diagnosed cases of Celiac disease on the basis of serology and/or histopathology were interviewed via a structured questionnaire via non probability consecutive sampling technique. Age, gender, age of diagnosis, diagnostic modality, extra intestinal features, gluten free diet status response recorded while ensuring patient anonymity. Data was entered and statistical analysis was performed via IBM SPSS version 25.</p><p><b><i>Results:</i></b> A total of 340 patients were enrolled in present study. Mean age of participants was 21.07 ± 12.99 years with predominantly female patients (62.9%). Most common Extra-intestinal feature was Anemia reported by 51.5% of patients (Figure I). Dermatological features and Malaise were significantly more common in patients with higher age. Anemia was significantly more likely in Female patients. Patients with stunted growth is significantly more likely to feel socially left out.</p><p><b><i>Conclusion:</i></b> Celiac Disease can present with multiple extra intestinal features. Most common feature was Anemia. Extraintestinal manifestations may have gender predisposition as Anemia is significantly more likely in female gender and age as Dermatological features were more likely in relatively older patients.</p><p><b>PP-03-261</b></p><p><b>Capsule endoscopy: Unfolding the diagnostic dilemma</b></p><p><b>Jitender Koli</b></p><p><i>army hospital research and refferal, New Delhi, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Capsule Endoscopy: Unfolding The Diagnostic Dilemma</p><p>KOLI Jitender; DHIMAN Priyank</p><p>Army Hospital Research and Referral ,Delhi</p><p><b><i>Objective:</i></b> The aim of this study was to reveal the usefulness of capsule endoscopy in diagnosing small bowel lesions in patients who presented with various indications in our Centre not diagnosed by conventional endoscopic techniques.</p><p><b><i>Material and Methods:</i></b> We studied the capsule endoscopy reports of 75 patients who presented to our center in last 1 year for evaluation of obscure gastrointestinal bleed,iron deficiency anemia , unintentional weight loss and patients of abdominal pain failed to diagnosed by convention bidirectional endoscopy.</p><p>Out of the 75 patients , 61 patients were of obscure gastrointestinal bleeding, 10 of Iron deficiency anemia ,3 of abdominal pain and 1 of unintentional weight loss.</p><p><b><i>Results:</i></b> In 30 patient we could not find anything and was reported as normal study.</p><p>7 out of 10 IDA patient,4 out of 4 abdominal pain,19 out of 61 obscure gastrointestinal bleed had normal study.</p><p>In 52 patient of obscure GI bleed 12 patients had duodenal and jejunal telengiectasias,6 had ileal telengiectasias,5 had small bowel erosions,4 had terminal ileal ulcers,5 had small bowel erosions,5 had jejunal ulcers,2 had jejunal polyp,2 had ascariasis and 1 had smalll bowel GVHD.</p><p>In 4 IDA patient 1 had ascariasis,2 had jejunal telengiectasia and 1 ileal ulcer.</p><p><b><i>Conclusion:</i></b> Capsule endoscopy is very useful tool for the diagnosis of small bowel lesion leading to obscure gastrointestinal bleeding, anemia. Utility is limited if used in patients of unintentional weight loss.</p><p><b>PP-03-276</b></p><p><b>A mucosal prolapse syndrome with preoperative suspicion of cancer treated by endoscopic mucosal resection</b></p><p><b>Takanori Suganuma</b></p><p><i>Suganuma Hospital, Iida, Nagano, Japan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Conservative treatment, such as improving defecation habits, is the first choice for treatment of rectal mucosal prolapse syndrome (MPS), but in cases refractory to treatment, excision of the MPS itself is an option.</p><p><b><i>Case Description:</i></b> 85 year old male. He has been bleeding hemorrhoids for a long time. Positive result of occult blood in stool at physical checkup. A total colonoscopy was performed by his previous physician. A 20 mm-sized SMT-like elevated lesion was found in contact with the dentate line in the rectum-anal canal region. The biopsy by the previous physician was Group 4, and the lesion was suspected to be a mucinous adenocarcinoma. Rectal examination revealed no mass and only a slight lump at 5 o'clock. Contrast-enhanced CT showed an area of abundant blood flow in the anal wall.</p><p>Cancer was suspected, but Miles surgery was too invasive and the patient was referred to our hospital. After a second lower endoscopy, NBI showed a Type 2B (JNET) structural pattern, and IDUS showed no evidence of deep invasion, so the patient underwent en bloc resection by EMR. Pathological examination showed varicocele formation across the dentate line, erosion and regeneration of the capsular epithelium, granulation of the stroma, and regenerative atypia of the epithelium, which was a finding of MPS associated with hemorrhoid. Hemorrhoidal bleeding improved.</p><p><b><i>Discussion:</i></b> In this report, we describe a case of MPS in which cancer was suspected preoperatively and hemorrhoidal bleeding symptoms improved after endoscopic resection.</p><p><b>PP-03-288</b></p><p><b>Contrast-enhanced Endoscopic-ultrasound improves diagnostic performance of EUS-guided-tissue-acquisition in solid pancreatic masses arising in chronic pancreatitis</b></p><p><b>Nikhil Sonthalia</b>, Awanish Tewary, Akash Roy, Uday Ghoshal and Mahesh Kumar Goenka</p><p><i>Institute of Gastrosciences and Liver diseases, Apollo Multispeciality Hospital, Kolkata, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Ancillary techniques for improving diagnostic accuracy of Endoscopic-ultrasound guided-tissue acquisition(EUS-TA) for solid pancreatic masses, are evolving. We aimed at evaluating role of contrast-enhanced EUS(CE-EUS) and EUS-Elastography(EUS-E) in improving diagnostic performances of EUS-TA for solid pancreatic masses.</p><p><b><i>Materials and Methods:</i></b> Patients with solid pancreatic masses undergoing EUS-TA using either standard B-mode-EUS alone or CE-EUS guidance from July 2023 to June 2024 were analyzed. Outcome measures were i) comparison of diagnostic performance between two modalities in all solid pancreatic masses and ii) comparison of diagnostic performance in pancreatic mass with chronic pancreatitis. Strain ratio obtained by EUS-E was also recorded for masses where CE-EUS was used. Diagnostic performance of use of combined strain ratio and enhancement pattern was also analyzed.</p><p><b><i>Results:</i></b> Out of total 94 patients, 62 underwent B-mode-EUS guided-TA and 32 underwent CE-EUS guided-TA.Overall for differential diagnosis, B-mode-EUS performed similar to CE-EUS. To diagnose malignant lesion in background of chronic pancreatitis, when compared to B-mode, CE-EUS had higher diagnostic accuracy(90.91%vs78.95%,p-0.0083), higher sensitivity (83.33 %vs50%,<0.0001) with similar specificity, similar positive predictive value and similar negative predictive value (table 1). Presence of combination of strain ratio above 6.24 and hypo-enhancement pattern on CE-EUS had sensitivity of 85% and specificity of 100% for predicting adenocarcinoma. When this combination was used in pancreatic masses with chronic pancreatitis, sensitivity increased to 100% for predicting adenocarcinoma.</p><p><b><i>Conclusion:</i></b> Advanced EUS modalities including contrast-enhancement and elastography should be used while performing EUS-TA for pancreatic masses with chronic pancreatitis as it improves diagnostic performance over standard B-mode EUS.</p><p><b>PP-03-290</b></p><p><b>Alteration in Gut Microbiota after Colonoscopy: Proposed Mechanisms and the Role of Probiotic Interventions</b></p><p><b>Yong Sung Kim</b></p><p><i>Wonkwang University, Digestive Disease Research Institute, Seongnam, South Korea</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Colonoscopy induces transient gastrointestinal symptoms and alterations in the gut microbiota. This study aims to review the alterations in the gut microbiota following colonoscopy, their possible mechanisms, and the effect of probiotics.</p><p><b><i>Materials and Methods:</i></b> We conducted a thorough literature search in the PubMed database in this review. For studies investigating alterations in gut microbiota after colonoscopy, we used search terms: (colonoscopy AND (microbiota OR microbiome) AND (dysbiosis OR effect OR alter OR change)). To identify studies examining the effects of probiotic use before and after colonoscopy, we used the search terms: (colonoscopy AND (probiotics OR probiotic)). We did not restrict publication year, language, or study design. Studies were screened for relevance based on titles and abstracts, and duplicates were removed.</p><p><b><i>Results:</i></b> Factors such as rapid colonic evacuation, increased osmolality, and mucus thinning caused by bowel preparation and exposure to oxygen during the procedure contribute to these alterations. Typically, the alterations revert to the baseline within a short time. However, their long-term implications remain unclear, necessitating further investigation. Split-dose bowel preparation and CO2 insufflation during the procedure result in fewer alterations in the gut microbiota. Probiotic administration immediately after colonoscopy shows promise in reducing alterations and gastrointestinal symptoms. However, the widespread use of probiotics remains controversial due to the transient nature of the symptoms and microbial alterations in the microbiota.</p><p><b><i>Conclusion:</i></b> Colonoscopy induces transient dysbiosis. Probiotics may offer greater benefits to individuals with preexisting gastrointestinal symptoms. Thus, probiotic administration may be a viable option for selected patients.</p><p><b>PP-03-305</b></p><p><b>Colorectal cancer incidence and mortality in Mongolia, 2018–2022</b></p><p><b>Yumchinsuren Tsedendorj</b>, Nomin-Erdene Davaasuren, Ganchimeg Dondov, Tsenguun Ganbat, Tsogzolmaa Shiirevnyamba, Tulgaa Lonjid and Batbold Batsaikhan</p><p><i>Institute of medical sciences, Mongolian national university of medical sciences, Ulaanbaatar, Mongolia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> We aimed to update data on the morbidity and mortality rate of colorectal cancer (CRC) among the population of Mongolia by province between 2018 and 2022.</p><p><b><i>Materials and methods:</i></b> This study was designed using a descriptive method. The data were collected from 21 general hospitals of provinces, 9 general hospitals of districts, and the National Cancer Center in 2018–2022. The incidence and mortality were calculated as mean annual numbers per 100,000 populations.</p><p><b><i>Results:</i></b> In the country, a total of 1316 (634 male) new cases were diagnosed and 782 deaths were caused by CRC in the last 5 years (2018–2022). The incidence of CRC in the last 5 years was 7.9 per 100,000 populations, and the mortality rate was 4.7 per 100,000. The provinces of Orkhon (12), Khentii (11), and Central (10) reported the highest incidences of CRC, whereas the provinces of Sukhbaatar (6.9), Selenge (6.6), Dornod (6), and Darkhan-Uul (6) had the highest death rates (per 100,000 populations). The incidence of CRC didn’t differ statistically significantly between men and women. Additionally, the estimated incidence has grown dramatically with patients’ ages.</p><p><b><i>Conclusion:</i></b> Our study presents evidence of a steadily increasing CRC incidence in Mongolia over the past five years. Therefore, it is necessary to determine the distribution of risk factors, learn from the experience of developed countries strategy for prevention, diagnosis, and treatment.</p><p><b>PP-03-312</b></p><p><b>Empirical Therapy Versus Susceptibility Testing-Guided Therapy for Refractory Helicobacter pylori Infection: A Randomized Controlled Trial</b></p><p><b>Jyh-Ming Liou</b>, Mei-Jyh Chen, Chieh-Chang Chen and Ming-Shiang Wu</p><p><i>National Taiwan University Hospital, Taipei, Taiwan</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background and Aims:</i></b> We aimed to evaluate whether optimized empirical therapy is non-inferior to susceptibility-guided therapy in treating refractory H. pylori infection.</p><p><b><i>Methods:</i></b> We conducted a multicenter, open-label, randomized controlled trial in Taiwan. Eligible patients were allocated to receive either empirical guided therapy or susceptibility testing guided therapy. The MICs were determined using the agar dilution test. Study participants received levofloxacin-based quadruple, bismuth quadruple therapy, rifabutin-based or clarithromycin-based concomitant therapies based on previous medication history or susceptibility testing results. The status of H. pylori was determined using the 13C-UBT at least 6 weeks after completing eradication therapy. The pre-specified margin for non-inferiority was set at 9%.</p><p><b><i>Results:</i></b> Among the 233 patients with refractory H. pylori infection who were randomly assigned and underwent post-eradication evaluation, the eradication rates for the empirical guided therapy group and the susceptibility testing guided therapy group were 82.6% (95% CI: 75.7%-89.5%) and 85.6% (95% CI: 79.3%-91.9%) in the ITT analysis (p = 0.533), respectively. In the PP analysis, the eradication rates were 82.5% (95% CI: 75.5%-89.4%) versus 87.1% (95% CI: 81.0%-93.2%) (p = 0.330). The difference in eradication rates between the empirical guided therapy and susceptibility testing guided therapy groups was -3.0% (95% CI: -11.0% to 5.0%, non-inferiority p = 0.107) in the ITT analysis, and -4.6% (95% CI: -12.5% to 3.0%, non-inferiority p = 0.179) in the PP analysis.</p><p><b><i>Conclusions:</i></b> Optimized empirical therapy demonstrated comparable efficacy to susceptibility testing guided therapy for treating refractory H. pylori infection.</p><p><b>PP-03-331</b></p><p><b>Enteric Glial Induce Tr17 Cells to Exert Immunosuppressive Effects in DSS Colitis</b></p><p><b>Jiahui Yang</b> and Shuixiang He</p><p><i>Department of Gastroenterology, The First Affiliated Hospital Of Xi'an Jiaotong University, Xi'an, China</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> RORγt+ Foxp3+ regulatory T (Treg) cells, known as Tr17 cells, are a novel subset of Tregs that modulate immune responses. The role of Tr17 cells in the pathogenesis of inflammatory bowel disease (IBD) is not well understood. Our previous studies demonstrated that enteric glial cells (EGCs), which may serve as potential antigen-presenting cells, can exert immunosuppressive effects by inducing Tregs. We further investigated the how EGCs affect Tr17 cells.</p><p><b><i>Materials and Methods:</i></b> Using C57 and EGC-ablated (GFAP-HSV-TK) mice, we induced DSS colitis. Some mice received fluorocitrate to inhibit glial metabolism. Immunofluorescence assessed the spatial relationship between cells. Flow cytometry (FACS) evaluated the proportions of Tr17, Foxp3+ Treg and CD39+ CD73+ T cells in mesenteric lymph nodes and colonic lamina propria. Cell co-culture assays evaluated Tr17 cell induction, and mixed lymphocyte reactions assessed their suppressive function. RNA-seq and PCR were conducted on EGCs post-co-culture to identify relevant molecular pathways.</p><p><b><i>Results:</i></b> EGCs were found in direct contact with Tr17 cells, which were significantly reduced upon EGC ablation, correlating with increased colitis severity. FC treatment did not notably affect Tr17 cell proportions or inflammation. In vitro, EGCs induced Tr17 cell through contact-dependent mechanisms, with Tr17 cells showing superior suppression of T cell activation compared to conventional Tregs. High expression of CD39 and CD73 was noted in Tr17 cells. Key molecules like I-CAM1, V-CAM1, and CCL2 and CCL7 may mediate these interactions.</p><p><b><i>Conclusion:</i></b> EGCs mitigate DSS colitis through contact-dependent induction of Tr17 cells, suggesting a novel pathway for immune regulation in IBD.</p><p><b>PP-03-332</b></p><p><b>Can impotence caused by Adalimumab be reversed? and Crohn’s be managed with alternatives like Ustekinumab</b></p><p><b>Asif Yasin</b>, Ali Qamar, Zeshan Pervaiz and Athesham Zafar</p><p><i>Walsall Manor Hospital Nhs Trust Uk, Walsall, United Kingdom</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Tumor Necrosis Factor-alpha (TNF-α) inhibitors, such as adalimumab, are widely used in treating autoimmune conditions. We present a case of erectile dysfunction (ED) induced by adalimumab, which resolved upon switching to Ustekinumab, another biologic drug.</p><p><b><i>Case Presentation:</i></b> A 50-year-old male with Crohn’s disease (CD) was treated with adalimumab. He developed progressive ED following the initiation of adalimumab. The Naranjo causality assessment score indicated a probable association between the TNF-α inhibitor and the adverse event.</p><p><b><i>Intervention:</i></b> Adalimumab was discontinued, and the patient was transitioned to Ustekinumab for continued disease management.</p><p><b><i>Outcome:</i></b> The patient's ED resolved after switching to Ustekinumab. Follow-up assessments confirmed sustained improvement in erectile function.</p><p><b><i>Conclusion:</i></b> This case suggests a potential link between TNF-α inhibitors and ED, highlighting the importance of considering such adverse events in clinical practice. The resolution of ED upon switching to Ustekinumab underscores the potential role of interleukin pathways in maintaining sexual function and suggests that ED may be specific to TNF-α inhibitors. Clinicians should remain vigilant for ED in patients undergoing TNF-α inhibitor therapy and consider alternative biologic agents when appropriate.</p><p><b><i>Keywords:</i></b> Erectile Dysfunction, Adverse Drug Reactions, Inflammatory Bowel Disease, Crohn’s Disease, Adalimumab, Naranjo Causality Assessment.</p><p>This case emphasizes the dynamic nature of therapeutic interventions in managing Crohn's Disease, highlighting the need for personalized treatment approaches. Further investigation into the mechanisms underlying these associations and the long-term safety profiles of different biologic agents is essential for optimizing patient care and minimizing treatment-related adverse events.</p><p><b>PP-03-333</b></p><p><b>Infliximab-induced seronegative Hepatitis Leading to Acute Liver Failure and liver transplant: A Case Report</b></p><p><b>Asif Yasin</b>, Zeshan Pervaiz, Athesham Zafar and Ali Qamar</p><p><i>Walsall Manor Hospital Nhs Trust Uk, Walsall, United Kingdom</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Infliximab, a TNF-α inhibitor used for autoimmune disorders like ulcerative colitis (UC), can occasionally cause drug-induced liver injury (DILI) and seronegative hepatitis. Unlike typical autoimmune hepatitis (AIH), this condition often lacks autoimmune antibodies. While most cases resolve positively, severe instances may lead to acute liver failure and require transplantation.</p><p>A 50-year-old woman with UC presented with abnormal liver function tests. She had no abdominal symptoms and her appetite and weight were stable. She had recently traveled to Tenerife and was treated with two doses of infliximab, being intolerant to thiopurines. There was no history of excessive alcohol consumption. Her liver function deteriorated rapidly, with bilirubin levels exceeding 100 and elevated ALT and ALP, despite a normal liver screen. A liver biopsy revealed seronegative hepatitis, leading to an orthotopic liver transplant within four weeks. Post-transplant, she is well on immunosuppressive medications.</p><p>This case highlights the complexity of managing IBD and potential infliximab-induced liver complications. Recognizing infliximab-induced hepatitis without autoantibodies and balancing effective IBD treatment with liver injury monitoring are significant challenges. A speculative model suggests that an unknown drug metabolite may become antigenic, causing hepatitis. Early recognition and diagnosis of drug-induced liver injury are crucial, emphasizing vigilant liver function monitoring in patients on immunomodulatory therapies and the development of diagnostic pathways to prevent severe complications.</p><p>Healthcare providers should monitor hepatic complications closely in patients on infliximab, promptly investigate abnormal liver tests, and consider early referral for specialized management if DILI is suspected.</p><p><b>PP-03-334</b></p><p><b>Hemorrhoid Ligation as an Effective Treatment Option for Hemorrhoids: An Evidence-Based Case Report</b></p><p><b>Ahmad Nur Aulia</b><sup>1,2</sup>, Camelia Camelia<sup>1</sup>, Muhammad Firhat Idrus<sup>1</sup> and Raymond Sebastian Purwanta<sup>2</sup></p><p><sup>1</sup><i>Pusat Endoscopy Saluran Cerna - RSUPN Dr. Cipto Mangunkusumo, Jakarta Pusat, Indonesia;</i> <sup>2</sup><i>Bali International Hospital, IHC Pertamedika, Sanur, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p>Hemorrhoids are swollen veins of the hemorrhoidal plexus characterized by symptoms resulting from prolapse and dilatation. The disease often presents with inflammation, pruritus, hemorrhage, and thrombosis. Several risk factors contribute to the development of hemorrhoids, including chronic constipation, low fiber diet, excessive straining, pregnancy, heredity, age, and occupation. The treatment varies with the prolapse grade, with grades I and II treated clinically. Grades III and IV, or those unresponsive to clinical therapy, often require more extensive treatments, ranging from non-operative and minimally invasive procedures to surgical techniques.</p><p>This study demonstrated a case of an 84-year-old woman presented with recurrent bloody stool with a history of osteoporosis, hypertension, and routine consumption of NSAID. The patient underwent a colonoscopy and received band-ligation treatment for internal hemorrhoids. This case underscores challenges in the treatment strategies of hemorrhoids. Endoscopic rubber band ligation merges traditional elastic ligation with endoscopic examination, allowing for improved control, photographic documentation, and sedation within a hospital setting. Endoscopic band ligation has recently been reported as an efficient method for treating symptomatic hemorrhoids grades II and III due to its low complication rates and high safety.</p><p><b><i>Keywords:</i></b> Hemorrhoids Internal, ligation, endoscopy</p><p><b>PP-03-340</b></p><p><b>Ileocolitis as a manifestation of Henoch-Schonlein Purpura: A case report</b></p><p><b>Yaditta Mirdania</b><sup>1</sup>, Mario Steffanus<sup>1</sup> and Muhammad Firhat Idrus<sup>2</sup></p><p><sup>1</sup><i>Fellow of gastroenterohepatology Departement of Internal medicine Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia;</i> <sup>2</sup><i>Division of Gastroenterology Departement of Internal medicine Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Introduction:</i></b> Henoch-Schonlein purpura is a form of immunoglobulin A vasculitis that is relatively rare in adults. This disease may have cutaneous and systemic manifestations, such as gastrointestinal, renal, and musculoskeletal involvements.</p><p><b><i>Case Description:</i></b> A woman, aged 64 years, complained of increasing reddish spots on both legs since 1 month prior to admission. The spots initially appear as red spots, then within 12 hours they widen and increase in number, without itching or pain. The patient also complained of bloody stool since 1 week prior to admission. On physical examination, the patient was afebrile, and vital signs were normal, the lower limbs were found multiple hyperpigmented macules with no tenderness. The Skin Biopsy Results was consistent with vasculitis, with visible damaged blood vessels surrounded by inflammatory cells, neutrophils and nuclear dust.</p><p>Colonoscopy Results was Ileocolitis caused HSP manifestation. Colonic Histopathology: Histology is consistent with low grade tubular adenoma, colonic dysplasia in active chronic ileocolitis with mild distortion. In this patient, a diagnosis of HSP was made with ileocolitis caused by HSP, and steroid therapy was given. Haematoschezia was reported to improve along with improvement of the skin lesions.</p><p><b><i>Discussion:</i></b> Gastrointestinal manifestations can be found in 44.7% of HSP sufferers, including abdominal pain, diarrhea and GI bleeding. Skin lesions usually be seen as the first manifestation of HSP, but in 10-15% of cases, gastrointestinal manifestation precede the skin lesions. Management of GI manifestations in HSP is oral steroids and supportive therapy.</p><p><b>PP-03-341</b></p><p><b>Manometric evaluation of Functional Constipation and Response to Biofeedback therapy: A tertiary Care Experience</b></p><p><b>Sujit Chaudhuri</b> and Agnibha Dutta</p><p><i>Manipal Hospital, Broadway, Salt, Lake, Kolkata, India</i></p><p>Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> In patients with refractory chronic constipation where organic causes have been ruled out, colonic transit study and anorectal manometry with balloon expulsion tests are important diagnostic tool to assess and categorise the disease. In patients with dyssynergic defecation (DSD), biofeedback therapy is treatment of choice. This study assess the prevalence of DSD in patients of Chronic idiopathic constipation and response of Biofeedback therapy in patients with DSD.</p><p><b><i>Materials and Methods:</i></b> Between January 2020 and May 2024, consecutive patients attending Gastroenterology OPD at Manipal Hospitals, Broadway has been evaluated as per standard protocol. Patients suspected to have functional defecatory disorders underwent anorectal manometry with balloon expulsion test. Patients willing to undergo biofeedback therapy received the same and treatment response was assessed.</p><p><b><i>Results:</i></b> Total 310 patients underwent anorectal manometry for chronic constipation. Mean age was 43±19 years. Of them 141 patients found to have DSD. Type I (129, 91,4%) was commonest. Twenty eight patients underwent biofeedback therapy with median 4 sessions (IQR=1). Type I DSD was in the majority (N=27). Twenty six patients had satisfactory response.</p><p><b><i>Conclusion:</i></b> DSD is a major cause of refractory constipation. Sessions of biofeedback therapy offers good outcome in this subgroup of patients.</p><p><b>PP-03-346</b></p><p><b>A Post-hoc Analysis : Association Between Metabolic Dysfunction-Associated Fatty Liver Disease Subgroups and Long-term Outcomes</b></p><p><b>Wah Loong Chan</b><sup>1</sup>, Nicholas Ming-Zher Chee<sup>1</sup>, Lee-Lee Lai<sup>1</sup>, Kee Huat Chuah<sup>1</sup>, Sanjiv Mahadeva<sup>1</sup>, Nik Raihan Nik Mustapha<sup>2</sup>, Sanjiv Mahadeva<sup>1</sup> and Wah Kheong Chan<sup>1</sup></p><p><sup>1</sup><i>Gastroenterology and Hepatology Unit, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;</i> <sup>2</sup><i>Department of Pathology, Hospital Sultanah Bahiyah, Alor Setar, Malaysia</i></p><p>Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Objectives:</i></b> Metabolic dysfunction-associated fatty liver disease (MAFLD) encompasses a diverse patient group. This study aims to assess liver disease severity and long-term clinical outcomes in adult MAFLD patients by subgroup classification.</p><p><b><i>Materials and methods:</i></b> This single-centre prospective study included MAFLD patients who underwent baseline liver biopsy. Analysis was conducted for three subgroups: MAFLD with type 2 diabetes (T2D) (group 1), MAFLD without T2D but were overweight or obese (group 2), and lean MAFLD without T2D who had at least two metabolic risk abnormalities (group 3).</p><p><b><i>Results:</i></b> Among 202 patients, 67.8%, 30.7%, and 1.5% were in groups 1, 2, and 3, respectively. The data for patients in group 1 [mean age 56.5±10.0 years old; male, 47.4%] and group 2 [mean age 47.4±10.8 years old; male, 48.4%] were analysed. The median follow-up was 7 (4–8) years. Group 1 patients were more likely to have metabolic dysfunction associated with steatohepatitis (MASH) (p <0.05), fibrotic MASH (p <0.05) and advanced liver fibrosis (p <0.001) compared to group 2 patients. Cumulative incidence of liver-related events, cardiovascular events, malignancy, and mortality of patients in group 1 were 0.69, 2.22, 0.83 and 0.69 per 100 person-years of follow-up, respectively, while in group 2, were 0, 1.43, 0.24 and 0.48 per 100 person-years of follow-up, respectively. The cumulative incidences were not significantly different between patients in group 1 and 2.</p><p><b><i>Conclusion:</i></b> T2D is associated with more severe liver disease in MAFLD patients, emphasizing the need for targeted screening and individualized management based on underlying metabolic abnormalities.</p><p><b>PP-03-348</b></p><p><b>The efficacy and safety of antidepressants and psychotherapy in irritable bowel syndrome: a meta-analysis</b></p><p>Zilin Ma and Guan Zhou Zhou and Xiaoyan Chi and Fei Pan</p><p><i>PLA General Hospital, Beijing, China</i></p><p><b><i>Objective:</i></b> To assess the efficacy and safety of antidepressants and psychotherapy for irritable bowel syndrome (IBS) patients.</p><p><b><i>Materials and Methods:</i></b> We conducted a literature search in Medline, Embase, Cochrane Library, and PubMed for randomized controlled trials (RCTs) up to June 2024, investigating the impact of antidepressants and psychotherapy on IBS versus placebo or control. A network meta-analysis with a multivariate consistency model random-effect metaregression was utilized. The primary outcome was the comparative effect of antidepressants or psychotherapy on overall IBS symptoms. Secondary outcomes included efficacy assessment based on antidepressant type or psychological therapy and adverse events.</p><p><b><i>Results:</i></b> This study included 74 RCTs (10,128 participants, 69% female): 32 studies compared antidepressants to placebo, and 42 compared psychological therapies to control, encompassing 22 different antidepressants and 36 psychological therapy modalities (Figure 1). Antidepressants generally showed greater efficacy than placebo (relative risk RR 0.84, 95% confidence interval CI 0.78-0.95). Tricyclic antidepressants (TCAs) were notably more effective in reducing overall IBS symptoms (RR 0.66, 95% CI 0.52-0.79), albeit with moderate heterogeneity (I2=49%, P=0.08). Adverse events were more common with antidepressants, but none were serious. Among psychological therapies, cognitive behavior therapy (CBT) was the most efficacious for IBS (RR 0.81, 95% CI 0.68-0.95). No significant differences were observed in adverse effect rates among the various psychotherapies and control therapy.</p><p><b><i>Conclusion:</i></b> Antidepressants have proven effective in alleviating IBS symptoms, with TCAs demonstrating the highest efficacy. Concurrently, sychotherapy, particularly CBT, emerges as a promising therapeutic approach. However, the evidence base is limited, potentially exaggerating reatment effectiveness.</p><p><b>PP-03-349</b></p><p><b>FMT is less effective in UC patients with depression, anxiety, insomnia or poor psychological resilience</b></p><p>Guanzhou Zhou<sup>1,2</sup>, Xiaolei Liu<sup>3</sup>, Ronghuan Jiang<sup>3</sup>, Hong Guo<sup>3</sup>, Yunsheng Yang<sup>2,4</sup> and Lihua Peng<sup>2</sup></p><p><sup>1</sup><i>School of Medicine, Nankai University, Tianjin;</i> <sup>2</sup><i>Clinical Division of Microbiota, Department of Gastroenterology and Hepatology, Microbiota Laboratory;</i> <sup>3</sup><i>Department of Medical Psychology, The First Medical Center of Chinese PLA General Hospital;</i> <sup>4</sup><i>National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China</i></p><p><b><i>Objectives:</i></b> Fecal microbiota transplantation (FMT) exhibited potential for remission induction in patients with ulcerative colitis (UC) while with uncertain efficacy. Those patients also suffered from abnormal psychological condition like depression and anxiety, which affected FMT efficacy. Therefore, we conducted a series of questionnaires about mental health in patients receiving FMT for UC to predict its effectiveness.</p><p><b><i>Materials and Methods:</i></b> Patients with active UC were enrolled in this study. Before the first FMT, four questionnaires were conducted and their cut-off values were based on their individual criterion. It included Patient Health Questionnaire depression scale (PHQ-9), Generalized Anxiety Disorder Scale (GAD-7), Insomnia Severity Index (ISI-7) and Connor-Davidson Resilience Scale (CD-RISC-25). The abnormal scores were defined as ≥5, ≥4, >7 and <70 individually.</p><p><b><i>Results:</i></b> All sixty-one patients with active UC received at least once FMT therapy. The mean Mayo score decrease from 7.95±2.15 to 5.18±2.69 (P<0.001). Patients with abnormal PHQ-9, ISI-7 and CD-RISC-25 scores decreased less in the Mayo assessment than patients with normal scores (1.885 ±2.142 vs. 3.457±2.571, P=0.014; 1.467±2.066 vs. 3.371±2.636, P=0.017; 3.600±3.043 vs. 1.550±2.188, P=0.027) (Figure1).</p><p>Additionally, we performed a logistic regression analysis to identify predictors of FMT efficacy. The abnormal scores on PHQ-9, GAD-7, ISI-7 and CD-RISC-25 indicated a poor response to FMT therapy. The regression model showed a 76.19% of specificity and 64.29% of sensitivity with 0.806 of AUC.</p><p><b><i>Conclusion:</i></b> Patients with abnormal PHQ-9, GAD-7, ISI-7 and CD-RISC-25 scores benefited less from the FMT treatment and those abnormal scores were promising predictors of poor response to FMT</p><p><b>PP-03-351</b></p><p><b>PROFILE OF COLORECTAL CANCER AT HAJI GENERAL HOSPITAL MEDAN IN 2019-2023</b></p><p><b>Dewi Murni Sartika</b><sup>1,2</sup>, Imelda Rey<sup>2</sup>, Taufik Sungkar<sup>2</sup>, Masrul Lubis<sup>2</sup> and Ilhamd Ilhamd<sup>2</sup></p><p><sup>1</sup><i>RSU Haji Medan, Medan, Indonesia;</i> <sup>2</sup><i>Haji Adam Malik General Hospital, Medan, Indonesia</i></p><p>Poster (Day 3), Poster Area, November 24, 2024, 8:30 AM - 5:00 PM</p><p><b><i>Background:</i></b> Colorectal cancer is the third most common cancer in the world and the second leading cause of cancer – related death. According to Globocan in 2012, the incidence of colorectal cancer in Indonesia was 12.8 per 100.000 adults with a mortality of 9.5% of all cancer cases.</p><p>The prevalence of colorectal cancer continuous to increase. This study aims to determine the characteristics of colorectal cancer patients at Haji General Hospital Medan as one of the referral hospital in North Sumatera Province.</p><p><b><i>Methode:</i></b> This retrospective study to the medical record of colorectal cancer patiens hospitalized at Haji General Hospital Medan in 2019-2023. The uncomplete data will be excluded from this study. The collected characteristics data was demographic, location of tumor, degree of differentiation. Univariate analysis was carried out using statistic program.</p><p><b><i>Result:</i></b> Total 102 colorectal cancer patients hospitalized in the last 5 years. Most patients were males (61,8%), average age 54 years old (33-78 years old). Most cancers located in the rectum (64,7%), left sided (20,6%), and right sided (14,7%). The degree of differentiation were obtained well-differentiated (58,8%), moderate-differentiated 23,5%, and poorly differentiated (17,7%)</p><p><b><i>Conclusion:</i></b> Colorectal cancer was most common among males (61,8%), located in the rectum (64,7%), and 58,8% well differentiated.</p>","PeriodicalId":15877,"journal":{"name":"Journal of Gastroenterology and Hepatology","volume":"39 S2","pages":"182-487"},"PeriodicalIF":3.7000,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jgh.16780","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Gastroenterology and Hepatology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jgh.16780","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0
PP-01-001
Long-term prognosis after ESD for superficial spreading esophageal squamous cell carcinoma of ≥50 mm
1Department of Gastroenterology, Hiroshima University Hospital;2Department of Molecular Pathology, Graduate School of Biomedical and Health Sciences, Hiroshima University
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To evaluate the outcomes and prognosis after ESD for superficial spreading esophageal squamous cell carcinoma (SESCC).
Subjects and Methods: We examined treatment outcomes (en bloc resection rate, tumor depth, lymphovascular invasion, and complications) in 79 patients with SESCC of 50 mm or more diameter and 79 lesions who underwent ESD between 2011 and 2018 at our department (observation period 86.9±33.2 months).
Results: En bloc resection rate was 99% (78/79). Tumor depth was pT1a-EP/LPM 45 (57%), pT1a-MM 16 (20%), pT1b-SM1 5 (6%), pT1b-SM2 13 (16%), lymphovascular invasion was observed in 12 (15%). No postoperative bleeding, intraoperative perforation in 4 (5%), and refractory stenosis (≥5 times endoscopic balloon dilatation) in 32 (41%) were observed. No local recurrence was observed, and 16 patients received additional treatment (CRT in 12, and operation in 4). Prognosis was 91%/84% for overall survival, 87%/80% for recurrence-free survival, and 77%/67% for disease-free surviva (3/5 years). Lymph node or other organ metastasis was observed in 6 patients (pT1a-MM,Ly0,V0/pT1b-SM1/pT1b-SM2 3/1/2). Death due to the primary disease was observed in 4 patients (pT1a-MM,Ly0,V0/pT1b-SM1/pT1b-SM2 2/1/1).
Conclusion: Although ESD for SESCC could be safely performed with en bloc resection, metastasis and primary death were observed in pT1a-MM lymphovascular invasion-negative cases that did not receive any additional treatment.
PP-01-002
A rare case of mycobacterium avium complex enteritis in advanced HIV
Isaac Chan1, Alyssa Sim2, Raymond Liang2, Hwei Yee Lee2 and Stephanie Sutjipto3
1National Healthcare Group, Singapore, Singapore;2Tan Tock Seng Hospital, Singapore, Singapore;3National Centre for Infectious Diseases, Singapore, Singapore
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Mycobacterium Avium Complex (MAC) infection is an AIDS-defining opportunistic infection seen in advanced human immunodeficiency virus (HIV). However, it rarely involves the gastrointestinal (GI) tract and endoscopic findings may be non-specific. We report a case of disseminated MAC infection with GI involvement in a patient with advanced HIV.
Case Description: A 43-year-old male with untreated HIV since diagnosis in 2016 presented with chronic, refractory diarrhoea and significant weight loss. His CD4 count was< 20 cells/uL. Evaluation of diarrhoea was negative for C.difficile toxin, stool cultures, microscopy and parasites. Computed tomography (CT) revealed hepatomegaly, diffuse lymphadenopathy and small-bowel thickening. Oesophagogastroduodenoscopy (OGD) revealed non-specific gastric erosions but showed diffuse, scattered white nodules involving the entire second part of duodenum with atrophic mucosa. However, normal mucosa was seen on sigmoidoscopy. Biopsies from abnormal duodenal mucosa, random gastric and rectal biopsies revealed diffuse foamy macrophages in the lamina propria, duodenal villous blunting and the presence of acid-fast bacillus organisms, without evidence of cytomegalovirus. This was also seen on lymph node biopsies. A diagnosis of disseminated MAC with GI involvement was made and treatment with rifabutin, azithromycin and ethambutol was initiated.
Discussion: 20-40% of patients with CD4 count < 50 will develop disseminated MAC infection. Rarely, there will be GI involvement with manifestations of diarrhea, abdominal pain or non-specific systemic symptoms. Endoscopic findings may not be typical. Vigilance and early recognition to obtain tissue for histology is crucial for diagnosis in suspected patients. Currently, the role of repeat endoscopy for treatment follow-up is unclear.
PP-01-003
An unusual case of rectal pain: migration of a decade-old IUCD, successfully managed endoscopically
Dulanja Senanayake1, Githma Wimalasena1, Duminda Subasinghe1,2 and Nilesh Fernandopulle1,2
1Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;2University Surgical Unit, National Hospital Sri Lanka, Colombo, Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Intrauterine contraceptive device (IUCD) is an effective and safe contraceptive method with few known complications which includes uterine perforation and migration. Here we present a unique case of an IUCD migrating across the vagina to the rectum and causing rectal pain and per-vaginal gas discharge, 10 years after insertion.
Case Description: A 38-year-old female presented with rectal pain and passing gas through vagina for 4 months. An IUCD was inserted 10 years back. Digital rectal examination revealed protruding rod like object anteriorly. Sigmoidoscopy showed a plastic tubular stump protruding through the anterior rectal wall. The loop was removed through the scope using a snare and the 3mm defect which appeared epithelialized was coagulated and closed with 3 endo-clips (Resolution 360TM Clip). The passage of gas from the vagina and rectal pain subsided and she remains asymptomatic 6 months post-intervention.
Discussion: Migration is a rare but recognized complication of IUCDs and should be suspected when the threads are not palpable on examination. Migration can occur to the bladder, omentum, intestines and rectum. They may cause significant complications such as peritonitis, bowel perforation, fistula and abscess formation. Retrieval can be done via hysteroscopy, endoscopy or laparoscopically depending on the location and complications. Endoscopic closure is successful in accessible sites like the intestines. Regular self-examination and follow-up are recommended for early detection. Endoscopic retrieval and closure of defects is a successful non-invasive method when the IUCD limbs are visible through the intestines.
PP-01-004
Beaver tail liver –Laparoscopic and computed tomographic views of a rare hepatic morphology
1Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;2Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;3University Surgical Unit, National Hospital Sri Lanka, Colombo;4Department of Radiology, National Hospital of Sri Lanka, Colombo
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Beaver tail of the liver(BTL) is a rare morphological lobar anatomical variant. It is characterized by extension of liver segment II/III beyond anterior margin of spleen. It is difficult to differentiate from spleen in imaging as echogenicity or density is similar and can lead to unnecessary investigations. This can mimic other pathologies like splenic subcapsular haematoma, peri-splenic fluid collections.
Case Description: A 62-year-old, ASA II male presented with anorexia, evening pyrexia and weight loss for one year. His serum biochemistry was normal except for an elevated erythrocyte sedimentation rate. Ultrasound abdomen showed splenomegaly and para-aortic lymph nodes suggestive of lymphoma. His CT abdomen incidentally detected BTL with the left lobe of liver encircling the spleen. He underwent a diagnostic laparoscopy for tissue diagnosis and revealed an additional extension liver parenchyma from left lateral segment of the liver encircling the spleen resembling the tail of a beaver. He had laparoscopic liver wedge biopsy for confirmation of the diagnosis.
Discussion: There can be ectopic, accessory lobes of the liver. One of the most commonly found accessory lobes corresponds to hypertrophied segment V/VI, best known as Riedel’s lobe. It is important to emphasize how the unexpected presence of the left hepatic lobe in the upper left quadrant of the abdomen can lead to misinterpretations in cross-sectional imaging. This case highlights the rare and unique challenges BTL presents to surgeons and radiologists and the importance of knowledge of rare hepatic anatomical variations in interpreting imaging studies.
PP-01-005
Hepatic tuberculosis mimicking hepatic metastases of a lymphoma –A rare case diagnosed with laparoscopy
Dulanja Senanayake1, Githma Wimalasena1, Sivasooriya Siwaganesh2,3 and Duminda Subasinghe2,3
1Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;2Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;3University Surgical Unit, National Hospital Sri Lanka, Colombo
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Hepatic tuberculosis(TB) without active pulmonary TB is quite rare. An estimate of the incidence is around 1% of all active TB cases. It manifests as thickened liver capsule with subcapsular nodular lesions and is referred to as “sugar-coated” or “frosted” liver.
Case Description: A 62-year-old, ASA II male presented with anorexia, evening pyrexia, and loss of weight for one year, with USS findings of splenomegaly and para-aortic lymph nodes and mediastinal lymphadenopathy on HRCT. His basic serum biochemistry was normal except for an elevated ESR of 100. His LDH was 105U/L (125-220). A CT abdomen was performed revealing multiple small non-enhancing hypoattenuating ill-defined lesions noted in both lobes of the liver, suggestive of lymphoma with involvement of the liver. A diagnostic laparoscopy was performed, and multiple bi-lobar liver lesions were found suggestive of secondary liver involvement of lymphoma. A wedge liver biopsy was performed which revealed granulomatous inflammation of the liver suggesting tuberculosis. Although the Acid-Fast stain was negative the patient was started on anti-TB regimen and the patient is currently asymptomatic and being followed up.
Discussion: This case report highlights the significance of taking TB into account as a potential cause in patients with lower gastrointestinal symptoms in TB-endemic areas by emphasizing the diagnostic challenges posed by hepatic tuberculosis with isolated liver involvement. To prevent serious complications of abdominal TB, early diagnosis and timely treatment is crucial. It also shows the importance of diagnostic laparoscopy and biopsy in view of early diagnosis.
PP-01-006
Clinicopathological features of colorectal cancer: A retrospective cross sectional study from tertiary referral hospital of Karachi
Nazish Butt, Nimrah Mehak, Ghulam Mohiuddin and Ghulam Haider
Jinnah Postgraduate Medical Centre, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Colorectal cancer (CRC) is a global health problem. It is cancer of colon, appendix and rectum. According to GLOBOCON 2018, It is the 4th most common cancer in the world and the third leading cause of cancer mortality.
Objective: To evaluate the clinicopathological features of CRC. Method: This was cross-sectional retrospective study conducted at the Gastroenterology Department of JPMC, Karachi from Jan. 2018 till Dec. 2022. All patients with CRC were included in the study. The data obtained was analyzed on the statistical software SPSS version 23.
Results: Total 1149 patients were enrolled with CRC and 566 patients were analyzed. The most common age group was 40 -60 years of age. The majority of patients were male 382 (64.7% ) Smoking had a strong association 51(9%) were smokers followed by Gutka 21 (3.7%). The common clinical presentation was abdominal pain 255(45% ), Bleeding per Rectum 178(31%), constipation 64( 28%), altered bowel habits 15.5% (n=88), diarrhea 8.3%(n=47), weight loss 64 (11.%). Colonoscopy revealed polypoidal growth in 387 (68.3%) of patients. The most common site of CRC was Rectum followed by Sigmoid colon 219(8.6%) and the least common site was Ascending colon 7(1.2%). Moderately differentiated Adenocarcinoma was the most common histological type found in 468(82.5%).
Conclusion: CRC is the leading cause of mortality. Most commonly involving the left side of colon. We need more studies to make guidelines for screening and surveillance of CRC in our population.
PP-01-007
Frequency and clinical characteristics of inflammatory bowel disease : A multicentre study from Karachi ,Pakistan
Nazish Butt and Omar Idris
Jinnah Postgraduate Medical Centre, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Inflammatory bowel diseases (IBD), including Ulcerative colitis (UC) and Crohn's disease (CD), are chronic gastrointestinal disorders influenced by genetic, environmental, immunological, and microbiological factors. Despite limited epidemiological studies in Pakistan, evidence indicates that IBD is increasingly prevalent in the region.
Aims: The aim of this study is to determine frequency and multifaceted clinicopathological features of IBD
Methods: This is an ongoing observational, cross-sectional study which is being conducted at, Jinnah Post Graduate medical Centre and National medical center, Karachi . All adult patients newly diagnosed with IBD were included in the study between January 2023 till date.
Results: The majority of our patients had UC (214, 97%) with a mean age of 38±18 years, equally prevalent in females 112(51%) and males 107(48%). The highest frequency was among the Sindhi community 38(17%), followed by Pathan 27(12%) and Punjabi 15(6%). A positive family history of IBD was seen in 89 (40%) patients, with 77 (35%) reporting per rectal bleed, and 66 (30%) experiencing abdominal pain and weight loss. Extensive colitis (E3) was present in 130 (54%) patients, severe disease in 120 (54%), with 42 (19%) having arthritis and 27 (12.3%) oral ulcers; 83 (37%) responded to Mesalazine and oral steroids, and 4 (1.8%) were referred to surgery
Conclusion: Ulcerative colitis, affecting adults aged 15-60 with no gender preference, often presents severely with extensive colitis and common extraintestinal symptoms like arthritis. Prompt diagnosis and early gastroenterologist involvement are crucial to prevent progression to colorectal cancer
PP-01-008
Colorectal carcinoma in young patients: Single centre study from Karachi, Pakistan
Nazish Butt and Ghulam Mohiuddin and Nimrah Mehak
Jinnah Postgraduate Medical Centre, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Colorectal cancer (CRC) is rising among young adults. According to GLOBOCON 2018, It is the 4th most common cancer in the world and the third leading cause of cancer mortality.
Objective: To evaluate the clinicopathological features of CRC among young adults.
Method: This was cross-sectional Retrospective study conducted at the Gastroenterology and Oncology Department of JPMC, Karachi from Jan. 2018 till Dec. 2022. All patients with CRC under age 30 were included in the study. Diagnosis was made on Colonoscopy and histopathology confirming CRC. The data obtained was analyzed on the statistical software SPSS version 23. Descriptive statistics were obtained by frequencies and percentages.
Results: Out of 566 patients of 117 young CRC patients were analyzed. The majority, 67 (57.3%) patients were male and 50 (42.7%) were female. Most common ethnic group were in Urdu speaking 47(40%). Smoking had a strong association 13(9%) were smokers. The common clinical presentation was abdominal pain 64 (53%) constipation 45 (38.5%) and Bleeding per Rectum 42 (36%).
Colonoscopy revealed polypoidal growth in 79 (67%) of patients followed by ulcerated lesions in 23 (19.7%). The most common site of CRC was Rectum affecting 48 (41%) followed by Sigmoid colon 46 (39.3%) and Ascending colon 7(2.6%). Moderately differentiated Adenocarcinoma was the most common histological type found in 96 (82.5%).
Conclusion: CRC is on rising among young adults, most commonly involving the left side of the colon. We need more studies to make guidelines for screening and surveillance of CRC in our population.
PP-01-009
Clinical and endoscopic features of solitary rectal ulcer syndrome: Multicenter study in Karachi, Pakistan
Nazish Butt1, Ghulam Mohiuddin1 and Amanullah Abbasi2
1Jinnah Postgraduate Medical Centre, Karachi, Pakistan;2National Medical Centre, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Solitary rectal ulcer syndrome (SRUS) is a chronic benign disorder characterized by the presence of an abnormality of the rectum in persons who have a long history of straining during defecation.
Methods: This was a retrospective observational longitudinal study, conducted in the department of Gastroenterology at Jinnah Post graduate Medical Center and National Medical Center, Karachi, from 1st March 2017 to till July 2024. All adult patients of either gender diagnosed with SRUS on basis of clinical history and endoscopic features were included in the study. The data obtained was analyzed on the statistical software SPSS version 26.
Results: There were total 85 patients in our study out of which 49(58%) were female and 36(42%) were male. Mean age was 35 ±17 years. Most common presentation was per rectal bleeding 68(80%). The most common predisposing risk factor was 85 (100%) self-digitation, 51(60%) prolong stay in toilet and rectal prolapse 5 (7.1%). Endoscopically most common finding was of single ulcer 80(95.7%). Histology revealed mucosal ulceration 75(88%) extension of muscular fibers 65 (77%), fibromuscular obliteration of lamina propria 59(70%) and crypt distortion 54 (64.3%). Associated psychological condition among patients were obsessive compulsive disorder 51 (60%) and anxiety disorder 46(52%). Most of the patients were treated through lifestyle modification and biofeedback therapy. Only 4 patients were referred to surgical intervention.
Conclusion: SRUS is a rare disorder with variable clinical presentation. This study revealed rectal self-digitation and psychological illness was the main risk factor for SRUS.
PP-01-010
Spectrum of colon polyps in patients presented to tertiary care hospital: Multicenter study in Karachi, Pakistan
Nazish Butt1, Ghulam Mohiuddin1, Ashfaque Ahmed2 and Amanullah Abbasi2
1Jinnah Postgraduate Medical Centre, Karachi, Pakistan;2National Medical Centre, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Colonic polyps are considered as premalignant conditions. Early detection and endoscopic removal of these precancerous lesions are very effective in reducing the incidence and mortality rate of colorectal cancer.
Methods: This was a prospective observational study, conducted in the department of Gastroenterology , Jinnah Post graduate Medical Center and National Medical Center, Karachi, from 1st March 2014 July 2024. Patients with either gender of any age were included in the study while patient with history of Colonic resection and pregnant were excluded. Polypectomy was done either by EMR technique, Hot/Cold snare or by Biopsy Forceps.
Results: There were total 210 patients in our study out of which 129(61%) were male and 81(39 %) were female. Mean age was 42 ±16 years. The most common type on gross examination was of large polyps 115(55.7%).Most commonly located in the sigmoid colon. Most common presentation was abdominal pain 145(69%) followed by per rectal bleed 105 (50%) and Constipation 70 (30%).Most common histological diagnosis were tubular adenoma 90(43%), followed by hyperplastic polyp 38 (18%), juvenile retention polyp 32 (15%), 9 (4.3%) Peutz Jaghers Syndrome and malignant polyps 21(10%).
Conclusion: Tubular adenoma were found in mostly colonoscopies followed by hyperplastic polyp which can develop into the cancer. Patients with adnominal pain and bleeding per rectum should undergo colonoscopies to rule out polyps.
PP-01-011
Inverted colonic diverticula-potential pitfalls in colonoscopy
Tsz Yui Chan
Department Of Surgery, New Territories West Cluster, Hospital Authority, Hong Kong, Hong Kong, Hong Kong
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Inverted colonic diverticula (ICD) are rare colonoscopy findings and occur in approximately 0.7% of people. This could possibly be under reporting due to the lack of awareness and limited cases presented as case reports only. This article aims to evaluate the incidence from a personal series prospective, features of inverted colonic diverticulum and possible methods that can help diagnose ICD.
Materials and Methods: Patients with colonoscopy findings of ICD were reviewed from 2015 to 2024 in the department of surgery, New Territories West Cluster, Hospital Authority, HKSAR.
Results: Total 2604 colonoscopies were performed by a single endoscopist in this time period and 5 patients were diagnosed with ICD (0.19%).
The mean age of the patients was 64.2 years old (ranging from 56 years old to 71 years old), with 3/2 male/female ratio. All (5/5) of the ICD were in the area of multiple colonic diverticula, and 60% (3/5) were located in the right-hemi colon. The features of ICD include elevated sessile ‘bubble wrap like’ appearance with fine concentric folds surrounding the lesion which enhanced with Methylene Blue. Gentle pressure with biopsy forceps can revert ICDs into typical diverticula. ICD cases were also reviewed by using Artificial Intelligence (AI) endoscopy system, sometimes failures in differentiating ICDs from polyps by AI were still encountered.
Conclusion: ICD is a rare intraluminal lesion that can be misinterpreted as elevated polypoid lesion. Meanwhile endoscopists’ experience may still be the most important factor in diagnosing ICD to prevent serious complications from ‘polypectomy’ for misdiagnosed ICDs.
PP-01-012
Optimized endoscopic submucosal dissection for colorectal lesions: A retrospective study of a prospective database
Jian Chen1 and Ruihua Shi1,2
1Southest University, Nanjing, China;2Zhongda Hospital affiliated to Southeast University, Nanjing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Early diagnosis and treatment are crucial in reducing related mortality of Colorectal cancer, but technical difficulties have limited the application of Endoscopic Submucosal Dissection (ESD) in Colorectal. Hybrid ESD is a simplified method, but has been reported with low en bloc resection rate and higher recurrence risk. Therefore, a modified method known as Optimized ESD was utilized to treat colorectal lesions.
The study aimed to evaluate the effectiveness and safety of Optimized ESD in treating colorectal lesions compared to Conventional ESD and Rescue Hybrid ESD.
Materials and Methods: The study included 277 colorectal mucosal lesions of ≥20 mm size treated with Optimized ESD, Conventional ESD, and Rescue Hybrid ESD. The primary outcomes included operation time and speed, while secondary outcomes included en bloc resection rate, postoperative complications rate, and recurrence rate.
Results: Optimized ESD was significantly better than Conventional ESD and Rescue Hybrid ESD in terms of operation time and speed(38.39 vs 60.19 vs 51.61min; P<0.001; 17.86 vs 14.68 vs 16.52 mm2/min; P=0.045). Optimized ESD was comparable to Conventional ESD and superior to Rescue Hybrid ESD in terms of en bloc resection rate(92.9% vs 96.3% vs 69.7; P<0.001). There was no significant difference in postoperative complications and local recurrence among the three groups.
Conclusion: Optimized ESD is a safe and effective technique for treating colorectal lesions, with a fast operation speed, short duration, satisfactory en bloc resection rate and recurrence rate.
PP-01-013
Study of colonic stent placement for ileocecal obstruction
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colonic stent placement in the ileocecal region is technically challenging, and clinical indications should be carefully considered. With only a few cases reported in the literature, we present the results of stent placement in the ileocecal region at our hospital.
Materials and Methods: We retrospectively reviewed 14 patients who underwent colonic stent placement for ileocecal obstruction at our hospital between September 2019 and April 2024.
Results: Among the 14 patients, five were bridged to surgery (BTS) and nine received palliative care. All patients experienced prompt relief of obstruction following stent placement and were able to resume eating. In all the five BTS cases, a stent of φ18 × 60 mm was used. No early complications such as perforation or migration were observed. Late complications included subileus before surgery, necessitating early hospitalization in three patients. The average time to surgery was 35 days, with all procedures performed laparoscopically in one-stage.
In the nine patients who received palliative care, stents of φ18 × 60 mm were used in four, φ18 × 100 mm in three, and φ18 × 120 mm in two. In one patient, microperforation was observed as an early complication; however, the patient’s condition improved with conservative care. As a late complication, two patients required stent replacement due to re-obstruction. The remaining seven patients experienced no stent-related complications before death.
Conclusion: Stent placement in the ileocecal region carries a significant risk of reocclusion. Prompt surgical intervention should be considered for patients undergoing BTS, as demonstrated in our patient cohort.
PP-01-014
Association between longer cecal intubation time and detection and miss rates of colorectal neoplasms
Ji Min Choi, Seon Hee Lim, Yoo Min Han, Jooyoung Lee, Eun Hyo Jin, Ji Yeon Seo and Jung Kim
Seoul National University Hospital, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: A longer cecal intubation time (CIT) occurs during colonoscopy under difficult insertion conditions, which may hinder meticulous mucosal observation. However, whether a longer CIT has detrimental effects on the detection of adenomas remains unclear. We aimed to evaluate the effects of CIT on the detection and miss rate of colorectal neoplasms in asymptomatic subjects.
Materials and Methods: Healthy examinees who underwent colonoscopy between March and July 2011 and August 2015 and December 2016 were retrospectively included. The primary outcome was the adenoma detection rate (ADR) across CIT quartiles; the secondary outcomes included the mean number of adenomas, advanced ADR (AADR), clinically significant serrated lesion (CSSP) detection, adenoma miss rate (AMR), miss rate of CSSPs and any colorectal neoplasms, and mean number of missed colorectal neoplasms in relation to CIT.
Results: Overall, 12,402 colonoscopy cases were classified into quartiles based on the CIT. The longer the CIT, the lower the ADR (P<0.001), AADR (P=0.004), and mean number of adenomas (P<0.001). The CSSP detection rate was not associated with CIT. In the follow-up colonoscopy, the AMR increased with increasing CIT (P=0.065). The missed rate of CSSPs (P=0.002) and any colorectal neoplasms (P=0.001) also increased with increasing CIT. In multivariate analysis, CIT was significantly associated with ADR, AADR, and AMR.
Conclusion: A longer CIT is associated with a lower ADR and higher AMR. Therefore, meticulous inspection is important for high-quality colonoscopies, especially in subjects requiring a longer CIT.
PP-01-015
Effect of ethyl acetate fraction from andaliman fruit extract on colorectal cancer cell line HT29
Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Me, Medan, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To evaluate the effect of the ethyl acetate fraction of andaliman (Zanthoxylum acanthopodium) fruit extract on colorectal adenocarcinoma cell line HT29.
Materials and methods: The extract of andaliman fruit was obtained through maceration of its simplicia. Chemical compound identification and antioxidant testing were performed, continued by assay of cytotoxicity by the MTT method, inhibition of G1 phase and G1-S phase cell cycle using Cyclin D1 and Cyclin E, and apoptosis induction using Caspase 3. These examinations were performed using flow cytometry.
Results: The IC value from the cytotoxicity test of andaliman fruit was 274,850 μg/mL. Administration of 1IC50 (274.85 μg/ml), ¾IC50 (206.14 μg/ml) from ethyl acetate fraction of andaliman fruit extract could decrease expression of Cyclin D1 or stopped the G1 phase cell cycle and could reduce the expression of cyclin E or inhibit the G1-S phase cell cycle. The IC50 from ethyl acetate fraction of andaliman fruit extract could increase Caspase 3 activity or could trigger apoptosis.
Conclusion: The ethyl acetate fraction of Andaliman fruit extract has potential as an anticancer agent by reducing cyclin D1, cyclin E expression and having the ability to induce apoptosis by increasing the activity of the Caspase 3 enzyme.
Keywords: Andaliman, extract, ethyl acetate, colorectal cancer
PP-01-016
The recurrence rate of advanced colorectal adenomas after polypectomy: A retrospective cohort study
Ma. Regina Dimaculangan and Marianne Linley Sy-Janairo
St. Luke's Medical Center - Global City, Taguig, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colorectal cancer is the third most commonly diagnosed malignancy worldwide. It develops in a multi-step manner from normal epithelium, to a pre-malignant adenoma, and into a malignant carcinoma, hence surveillance colonoscopy is necessary. This study aims to determine the association of high-grade adenoma features and its risk of recurrence. This is critical in early diagnosis and treatment, thereby increasing the chance for good clinical outcomes.
Materials and Methods: Eligible adult subjects are selected from the pathology data bank, and daily in-hospital census, who have advanced polyps detected during their first colonoscopy, and with at least 2 colonoscopies done from January 2019 to December 2023 in St. Luke's Medical Center - Global City, a tertiary hospital in the Philippines.
Results: This study included 71 adult patients, with a mean age of 63 years old, and are mostly males. Among the high risk features, the following showed statistically significant p-values that contribute to the recurrence rate of advanced polyps: Family history of colon cancer (HR 1.59, 95% p=0.003), Polyps more than 4mm in size (HR 1.80, p=0.04), More than one high polyp identified (HR 1.26, p=0.04), BPPS with a score 1 (HR 1.78, p=0.03), Tubulovillous morphology (HR 1.59, p=0.03), and Severe dysplasia (HR 1.98, p=0.02). The recurrence years show that the risk is significantly higher within the first 1-2 years post-polypectomy (HR 1.16, p=0.003).
PP-01-017
Comparison of in-hospital and home-administration of bowel preparation agent before outpatient colonoscopy in elderly patients
1Division Of Gastroenterology, Department Of Internal Medicine, Faculty Of Medicine, Saga University, Saga City, Japan;2Department of Gastroenterology, Ureshino Medical Center, Ureshino, Japan;3Nursing Department, Ureshino Medical Center, Ureshino, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Most patients come to the hospital after taking a bowel preparation agent at home before an outpatient colonoscopy. However, some elderly patients take a bowel preparation agent in the hospital after coming to the hospital for a colonoscopy. This study aimed to compare in-hospital and home use of a bowel preparation agent in elderly patients.
Materials and Methods: Patients aged 70 years or older who took bowel preparation agent (Moviprep®) before outpatient colonoscopy either in-hospital or at home from September 2022 to September 2023 were prospectively enrolled in Ureshino Medical Center. The primary endpoint was the bowel preparation time before the start of colonoscopy.
Results: One hundred fifty-one patients (51 in group A and 101 in group B) were enrolled. The mean age was 82.6 ± 5.3 years in Group A and 75.6 ± 3.8 years in Group B (P<0.001). The percentage of patients with ASA-PS:0 was significantly lower in Group A (84.3%) and Group B (98.0%) (P=0.003). The bowel preparation time was 203.9 ± 73.8 minutes in Group A and 270.3 ± 61.5 minutes in Group B (P<0.001), significantly shorter in Group A. Colonoscopy time was similar in both groups (Group A: 29.3 ± 13.7 min vs. Group B: 29.1 ± 12.4 min; P=0.943), and there was no significant difference in adverse events between the two groups.
Conclusions: In elderly patients with low PS, in-hospital administration of a bowel preparation agent can shorten the bowel preparation time and ensure the same safety as home administration.
PP-01-018
Deep neural networks for confirming cecum achievement during screening colonoscopy
Sabina Guseinova
Yaroslavl Cancer Hospital, Yaroslavl, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Screening colonoscopy is the gold standard for preventing colorectal cancer. An AI approach can be helpful for confirming cecal achievement and monitoring colonoscopy quality.
Materials and Methods: A digital database was created with 2,671 hand-labeled images from SC collected from more than 250 patients. 2,294 images were negative (without the appendiceal orifice) and 377 were positive (containing the AO). The database was randomly divided into a validation set (80%) and a training set (20%). The validation base had 2,136 images, including 311 positive and 1,825 negative. The training set had 535 images, including 66 positive and 469 negative. For training, we used a ResNet50 convolutional neural network pre-trained on the ImageNet dataset.
Results: The following results were obtained on a test dataset in the process of the study - the best result on the validation set was AUC = 0.97, and the best value is F1-score = 0.85, when a threshold is th = 0.608. Then the trained model was checked on a test set the area under the curve is equal to AUC = 0.95, F1-score equal 0.9 with a threshold th = 0.462. (table 2) The average analysis time of one image is 29 ms, which allows to process up to 40 images per second.
Conclusion: We have developed and clinically tested an algorithm based on a DNN for object classification on endoscopic images to confirm cecum achievement with high accuracy. These results can be integrated into a quality control system, reducing subjective medical errors during SC.
PP-01-019
Application of automated deep learning for diagnosing high-risk adenomas in colorectal polyps ≤10mm
Da Yeon Ham, M.D. Hyun Joo Jang, M.D. Sea Hyub Kae and Jae Gon Lee
Division of Gastroenterology, Department of Internal Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to develop a computer-aided diagnosis (CADx) model using an automated deep learning program to classify low- and high-risk adenomas among colorectal polyps ≤10 mm with standard white-light imaging (WLI) endoscopy.
Methods: Still images of colorectal adenomas ≤10 mm were extracted. High-risk adenomas were defined as high-grade dysplasia or adenomas with villous histology. Neuro-T version 3.2.1 (Neurocle Inc., Seoul, Korea), an automated deep learning software, was used for deep learning. Accuracy, precision, recall, and F1 score of the deep learning model were calculated. Endoscopy expert and trainee were invited to diagnose endoscopic images to compare their diagnostic accuracy with that of the deep learning models.
Results: A total of 2,696 endoscopic images (2,460 low-grade and 236 high-grade adenomas) were used for training of the deep learning model. In classifying high- and low-risk adenomas in the validation dataset (323 low-grade and 35 high-grade adenomas), the model demonstrated 95% accuracy, 85.2% precision, 87% recall, and 86.1% F1 score overall. The area under the receiver operating characteristic value for classifying high- and low-risk adenomas was 0.936 and 0.925, respectively. The expert endoscopist and endoscopy trainee showed an overall accuracy of 96.6% and 89.7%, respectively, for discriminating high- and low-risk adenomas in the validation dataset.
Conclusion: A CADx model established by an automated deep learning program showed high diagnostic performance in differentiating high- and low-risk adenomas among colorectal polyps ≤10 mm using only WLI. The performance of the model was comparable to the expert and superior to the trainee.
PP-01-020
The risk stratification of delayed postpolypectomy bleeding in patients with and without chronic kidney disease
Hye Kyung Hyun, Hae-Ryong Yun and Cheal Wung Huh
Yonsei University College Of Medicine, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The association between colonoscopic delayed post-polypectomy bleeding (DPPB) and chronic kidney disease (CKD) remains unclear. We aimed to elucidate whether CKD population are at increased risk of DPPB compared with non-CKD population.
Methods: A large cohort study involved patients who underwent colonoscopy and polypectomy in Korea between 2005 and 2022. We extensively collected various covariates, including patient-related, polyp-related, and procedure-related factors. We conducted large propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) to determine whether CKD affects the occurrence of DPPB. The primary outcome was the association CKD and risk of DPPB, and the secondary outcomes were the risk of DPPB depending on the stage of CKD.
Results: Among whom 16,885 patients and their respective 41,994 polyps were included in the analysis. Of these, 2,351 individuals (13.9%) were diagnosed with CKD, categorized as follows: 894 (38.0%) in early stage (stages 1,2), 1,457 (68.0%) in advanced stage (stage 3-5); the number of non-CKD population was 14,534. The risk of DPPB in patients with CKD was significantly higher compared to those in the non-CKD group (OR 1.532, CI 1.040-2.257, P=0.031). After large-scale PSM and IPTW, the risk of DPPB was significantly increased according to the stage of CKD (OR 2.375, 95% CI 1.001–5.636 for early stage and OR 2.797, 95% CI 1.201–6.512 for advanced stage, all P<0.05).
Conclusions: CKD independently increased the risk of DPPB, even in early stages, and was associated with progression to advanced CKD. Careful monitoring during polypectomy is essential at all CKD stages.
PP-01-021
Impact of procedure sequence on cecum insertion time in same-day bidirectional endoscopy: Trainee versus expert
Hyeong Ho Jo, Eun Young Kim, Joong Goo Kwon, June Hwa Bae, Kang Ho Lee, Jae Jin Lee, Juseok Lee and Gwanghyo Yim
Daegu Catholic University School of Medicine, Daegu, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The optimal sequence for same-day bidirectional endoscopy remains controversial. This study aims to further investigate the impact of procedure sequence on cecum insertion time, with a particular focus on differences between experts (≥10 years) and trainees (<1 year). We hypothesized that abdominal gas insufflation from esophagogastroduodenoscopy (EGD) could impact colonoscopy (CF), specifically prolonging cecal insertion time in trainees.
Materials and Methods: This retrospective cohort study was conducted at Daegu Catholic University Medical Center from March 2023 to February 2024. A total of 1095 CF cases were included, with 655 performed by experts and 440 by trainees. Among the expert cases, 269 followed EGD first, and 386 followed CF first. In the trainee group, 236 followed EGD first, and 204 followed CF first. Cecum insertion times were compared between these groups.
Results: In the expert group, the cecum insertion time was 8.6 minutes for the EGD-first group and 8.0 minutes for the CF-first group, indicating no significant difference (p = 0.127). In the trainee group, the cecum insertion time was 13.8 minutes for the EGD-first group and 12.2 minutes for the CF-first group, showing a statistically significant difference (p = 0.011).
Conclusion: This study demonstrates a significant difference in cecum insertion times between the EGD-first and CF-first procedures in the trainee group but not in the expert group. These findings suggest that performing EGD before CF can increase cecum insertion time for trainees, highlighting the need for protocol considerations in training programs. No significant impact was observed for experienced practitioners.
PP-01-022
Meteospasmyl® (alverine citrate plus simethicone) improving the tolerability of bowel preparation for colonoscopy
Anastasiia Kasikhina, Sergei Kashin and Dmitrii Zavyalov
Yaroslavl State Cancer Hospital, Yaroslavl, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Colonic dysmotility and spasm are often causing not only difficulties in scope insertion, patient’s discomfort and pain during colonoscopy but also affecting stool transit and patient tolerability during bowel preparation.
A prospective, single-center, comparative, randomized study was conducted to assess the effect of Meteospasmyl® on the tolerability and effectiveness of preparation for colonoscopy in increased risk of poor-quality bowel cleansing patients. 65 patients aged 32-69 years were randomized in two groups: group I–osmotic sulphate-based laxative Eziсlen® split-regimen preparation (n-32); group II-Eziсlen® split-regimen preparation+Meteospasmyl®(n-33) administered 1 capsule 2 times on the day before colonoscopy and 1 capsule in the morning the day of the exam.
Spontaneous peristalsis and spasm during colonoscopy prevent the endoscope from moving forward. Spasm aggravates bowel transit, which reduces the quality of preparation and increases the total time of colonoscopy. We found a decrease in the total time of colonoscopy in Meteospasmyl® group (12.4min vs 14.8min (p = 0.04) as well as the time of colonoscope insertion. "Excellent" bowel preparation in Meteospasmyl® group was in 43.8%. Bloating during preparation was observed in 12% compared with 3.1% in Meteospasmyl® group(p<0.001). The overall tolerability of preparation using five-point Likert scale was assessed as excellent in 91% of Meteospasmyl® group and in 76% without Meteospasmyl®(p = 0.02).
Novel approach for overcoming colonic spasm is peroral antispasmodic agent Meteospasmyl®, which improve tolerability of bowel preparation, reduce bowel dysmotility, time of colonoscope insertion and provide high-quality colonoscopy.
PP-01-023
Effectiveness of colonoscopy on colorectal cancer incidence among persons 40-49 years
Hyun-soo Kim and Su Young Kim and Hyunil Kim
Yonsei Wonju Severance Christian Hospital, Wonju, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and Aim: There are few data on the preventive effect of colonoscopy at ages 40-49 years at the population level. In this study, the risk of CRC according to the presence or absence of CSP in their 40s was evaluated in a retrospective cohort design.
Methods: Using the Korean National Health Insurance System data between 2004 and 2006, we constructed 1:5 colonoscopy cohort (CsC) and non-colonoscopy cohort (NCsC) at ages 40-49 years and followed through 2020. A total of 2,940,792 subjects were enrolled and CRC risk was evaluated after propensity score matching between CsC and NCsC based on age, sex, smoking, metabolic syndrome (MetS) and potentially relevant variables.
Results: Among 490,132 CsC subjects and 2,450,660 NCsC subjects at baseline periods in 40-49 ages, 689 (0.14%) and 9,440 (0.39%) cases of CRC were found during 10 years follow up period, respectively. According to age group, cumulative CRC incidences in early 40’s (40-44) were 0.10% in CsC and 0.25% in NCsC respectively. For late 40’s (45-49), those were 0.17% in CsC group and 0.49% in NCsC group, respectively. Compared with NCsC, the adjusted hazard ratios (aHRs) for CRC development in CsC group were 0.42 (men, 95% CI: 0.35-0.50) and 0.37 (women, 95% CI: 0.30-0.47) in early 40’s and 0.33 (men, 95% CI: 0.29-0.37) and 0.39 (women, 95% CI: 0.33-0.45) in late 40’s.
Conclusion: Overall, colonoscopy in their 40s significantly lowered the risk of colorectal cancer development by 58-67%, suggesting the rationales for optimization of age and gender to start colonoscopy.
PP-01-024
A Comparative study of oral sulphate solution and polyethylene glycol in bowel preparation for colonoscopy
Ji Liu, Yanjun Chen, Fujuan Luan and Jianying Lu
The First Affiliated Hospital Of Soochow University, Suzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study compared the efficacy of 3 liters of Oral sulfate solution (OSS) and 3 liters of Polyethylene glycol (PEG) in bowel preparation for colonoscopy.
Materials and Methods: This study retrospectively included 1140 participants who underwent colonoscopy at the First Affiliated Hospital of Soochow University from June 9 to October 26, 2023. Participants were divided into OSS group and PEG group(570 participants per group). The efficacy of bowel preparation was evaluated based on the Boston Bowel Preparation Scale (BBPS).
Results: There were no significant difference in baseline information between two groups (P>0.05). The bowel preparation success rate was correlated with the type of colonoscopy prep laxatives, gender and diabetes(P<0.05). Bowel preparation success rate in OSS group was higher than that in PEG group (92.3% vs 84.4%, P<0.001). The total BBPS score and scores of each colon segment in OSS group were higher than those in PEG group (P<0.05). There were no difference in bowel movement frequency and adverse reaction incidence rate between both(P >0.05). Additionally, in PEG group,the main factors of the bowel preparation success rate were gender and diabetes,and the bowel preparation success rate of women was 2.025 times that of men (P=0.018),and the bowel preparation success rate in diabetic patients was reduced by 58.8% compared to non-diabetic patients (P= 0.044). There were no significant factors of bowel preparation success rate in OSS group (P > 0.05).
Conclusion: 3L OSS has more advantages over 3L PEG in bowel preparation for colonoscopy, with a higher bowel preparation success rate.
PP-01-025
Explainable AI model for colorectal polyp detection and classification based on wasp and attention mechanism
先生 Haoxiang Ni
The First Affiliated Hospital of Soochow University, Suzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To develop an interpretable classification model for colorectal lesions to assist endoscopists in learning and clinical decision-making.
Materials and Methods: We created a Fast RCNN_Resnet50v2_ATT model for detecting and classifying colorectal polyps. The model integrates an attention mechanism to boost precision. It addresses the WASP classification's seven binary questions, synthesizes responses with a Random Forest algorithm for final pathological judgment (HP/AD/SSL), and outputs findings to aid endoscopist comprehension.
We also trained models using direct pathological typing to emulate non-interpretable models, comparing their accuracy with our model and Suzhou endoscopists on an external test set.
We utilized the POLAR dataset from Amsterdam for training and validation, applying Chinese endoscopic images for testing.
Results: The model excels in adenoma with high accuracy (0.89), recall (0.85), and F1 score (0.87). However, it struggles with differentiating hyperplastic polyps and serrated lesions, showing respective scores of 0.55/0.86/0.67 and 0.85/0.56/0.68. These challenges mirror past issues with WASP classification by endoscopists. We plan to refine the model, focusing on the attention module's contribution.
PP-01-026
Development of a smartphone application by artificial intelligence to predict bowel preparation quality for colonoscopy
Shoko Ono1, Yuji Ono2, Mio Matsumoto3, Erina Ishibe4 and Masaki Inoue1 and Naoya Sakamoto
1Hokkaido University Hospital, Sapporo, Japan;2Sapporo City General Hospital, Sapporo, Japan;3Sapporo Cancer Screening Center, Public Interest Foundation Hokkaido Cancer Society, Sapporo, Japan;4Hokkaido University Graduate School of Medicine, Sapporo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The quality of a colonoscopy (CS) is heavily dependent on the quality of bowel preparation. This study aimed to develop an artificial intelligence (AI) system within a cloud computing environment to automatically assess bowel preparation adequacy in real time.
Materials and Methods: Images of stools taken by patients during preparation for CS were collected and annotated as either complete or incomplete. An AI system was trained on this annotated dataset and integrated into a smartphone application. The application was configured to activate the camera upon scanning a unique QR code assigned to each patient. Patients then photographed the stool in the toilet bowl, and the AI immediately determined the adequacy of the bowel preparation, displaying the result on the screen. The accuracy of the AI system was evaluated using a test set of images and compared with visual judgments made by blinded endoscopists.
Results: The training set consisted of 107 images with complete preparation and 69 images with incomplete preparation. The test set consisted of 135 images. The application demonstrated an accuracy of 90.4% (95% CI: 84.2−94.3). The sensitivity and specificity were 91% (95% CI: 84.2−94.3) and 89.5% (95% CI: 82.2−94.1), respectively.
Conclusion: An AI-driven application for the real-time evaluation of bowel preparation quality for colonoscopy has been developed. A clinical trial is currently ongoing to further assess its efficacy.
PP-01-027
Investigation for the clinicopathological characteristics of post-colonoscopy of colorectal cancer(PCCRC) at our hospital
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: The purpose of this study was to investigate the clinicopathological characteristics of PCCRC at our hospital.
Methods: Among 880 colorectal cancer cases (excluding Tis) diagnosed at our hospital from January 2018 to December 2022, PCCRC was defined as cases detected after endoscopy without colorectal cancer diagnosis within the past 3 years.
Results: Eight cases of PCCRC were identified during the observation period. The mean age at diagnosis was 72.3 years, and the male-to-female ratio was 6:2. The localization was 2:1:1:0:4:0 in C:A:T:D:S:R. The mean lesion size was 25.3 mm, and the gross appearance were Isp, IIa, IIa+IIc, and IIc+IIa in one case each, and type2 in four cases. The main histological types were tub1 and tub2 in 4 cases each, with mixed por and muc in 1 case each. The depth of the lesions was 2:1:2:2:1 for T1a, T1b, T2, T3, and T4b, and the stage classification was 5:1:2 for I, IIIb, and IV. The mean time from the previous endoscopy to the diagnosis of PCCRC was 24.8 months. Two cases were referred from other hospitals for polyp resection, and multiple polyps were resected. The average colonoscopic withdrawal time was 19.3 minutes, but in 2 cases the withdrawal time was shorter at 3 minutes.
Conclusion: Some PCCRC cases at our hospital were found in advanced cancers including metastatic lesions. The results suggest that it may be necessary to pay attention to the cases referred for endoscopic treatment, especially involving treatment of multiple lesions, and withdrawal time.
PP-01-028
The Effectiveness of artificial intelligence-based colonoscopy: Propensity score matching study
1Inje University Busan Paik Hospital, Busan, South Korea;2Pusan National University Hospital, Busan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Adenomas, accounting for over 70% of colon cancer origins, make their detection during colonoscopy a critical procedure in colon cancer prevention. Artificial intelligence based on deep learning system has been developed and is under study in countries such as the United States, Japan, and China. This study aims to evaluate the effectiveness of the domestically developed deep learning system-based artificial intelligence, Endoscopy as AI-powered Device (ENAD).
This study evaluates ENAD's efficacy by retrospectively analyzing 654 colonoscopy cases, excluding 197. Among them, 104 cases used ENAD, and 353 did not. Propensity score matching was used to reduce selection bias.
Baseline characteristics such as age, gender ratio, Body Mass Index (BMI), obesity ratio, American Society of Anesthesiologists (ASA) score, Boston Bowel Preparation Scale, indications for colonoscopy, and practitioner experience did not significantly differ between the ENAD-assisted and non-assisted groups post-matching. Post-colonoscopy outcomes revealed that the ENAD-assisted group showed higher average polyp detection rates and adenoma detection rates compared to the non-assisted group, although these differences were not statistically significant before or after matching.
Although the ENAD-assisted group exhibited higher average polyp and adenoma detection rates post-colonoscopy, these differences were not statistically significant. Consequently, the study concludes that ENAD did not significantly improve adenoma or polyp detection rates compared to conventional colonoscopy. Further research with a larger sample size and detailed analysis of medical histories and colonoscopy indications is needed to fully understand the potential benefits of AI-assisted technologies in colon cancer detection and prevention.
PP-01-029
A comparison between 1 L polyethylene glycol and oral sodium sulfate tablet for bowel preparation
Jin Hwa Park1, Jeong-Sik Byeon2 and Su Hyun Park2
1Hanyang University, Seoul, South Korea;2Asan Medical Center, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and Aims: A low-volume (1 L) polyethylene glycol plus ascorbic acid (PEG-A) solution and oral sodium sulfate tablet (OST) are recently introduced agents for colonoscopy bowel preparation. This study investigated the efficacy, safety, and tolerability of 1 L PEG-A versus OST.
Methods: This single-center, prospective, randomized, endoscopist-blinded study randomly assigned patients into 2 groups: 1 L PEG-A (group A); and OST (group B). Efficacy of bowel preparation was assessed using the Boston Bowel Preparation Scale (BBPS). Tolerability and safety were investigated using a standardized questionnaire.
Results: A total of 174 patients were included in the final analysis (group A, n=92; group B, n=82). Successful bowel preparation was achieved in 91.3% and 95.1% of patients in groups A and B, respectively (p=0.324). Overall mean satisfaction with bowel preparation was greater among those in group B versus vs. those in group A (8.2±1.7 vs. 6.8 ± 2.0, respectively; p<0.001). Although abdominal distention was less common in group A than group B (3/92 [3.3%] vs. 9/82 [11.0%], respectively; p=0.045), overall adverse events developed similarly in both groups (27/92 [29.3%] vs. 21/82 [25.6%], p=0.583). In subgroup analysis of older patients (≥ 65 years of age), efficacy, overall satisfaction, and safety profiles were not different between groups A and B.
Conclusions: Both 1 L PEG-A and OST demonstrated efficacy, tolerability, and safety for colonoscopy bowel preparation. OST was slightly better tolerated, whereas 1 L PEG-A resulted in less abdominal distention. Both agents were effective and safe in older patients.
PP-01-030
Endoscopic stricturotomy of anastomotic strictures following ileo-caecal resection in Crohn’s: First New Zealand Case Series
Rajan Patel and Mehul Lamba
Christchurch Hospital, Christchurch, New Zealand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Endoscopic stricturotomy (EST) is a novel endoscopic technique for the management of IBD-related strictures. There are no published reports of this procedure from New Zealand.
Materials and Methods: We describe the demographics, stricture criteria and early outcomes of 6 cases that underwent EST at a large tertiary hospital in NZ.
Results: 6 patients with colonoscopy or radiologically confirmed anastomotic strictures at the site of previous ileo-caecal resection for Crohn’s disease have been included. 2 endoscopists performed the procedures. Mean age 49.1 (range 25 to 72), Female 40%. Mean interval between most recent surgery and endoscopic intervention 12.2 years (range 2 to 30 years). No immediate or delayed complications, surgery or hospital admissions at 3 months follow up. No repeat endoscopic interventions were required during the follow up period.
Conclusion: EST appears to be a safe and efficacious approach to short anastomotic strictures in Crohn’s disease. Longer range follow up and further work to investigate optimised medical management following EST is required.
PP-01-031
Bowel preparation quality for colonoscopy - Does distance make the colon grow dirtier?
Callum Hill, Harry White, Rajan Patel and Jonty Morreau
Christchurch Hospital, Christchurch, New Zealand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Aotearoa has an important mix of urban and rural populace resulting in challenging deliverance of healthcare. Increased distance to health services negatively affects patient outcomes, including international literature suggesting poorer bowel preparation quality. We aimed to investigate the effect of distance to an endoscopy unit on the quality of bowel preparation in an Aotearoa population.
Materials and Methods: A retrospective study was undertaken of the colonoscopies conducted at a large tertiary hospital between August 2023 and October 2023. The distance and expected travel time between patient’s home and endoscopy unit was calculated. Quality of bowel preparation, assessed with the Boston bowel preparation scale (BBPS), at the time of colonoscopy, was recorded. Adequate bowel preparation was defined as BBPS 6 or above. Data was also collected on gender, ethnicity, age, comfort score, indication and number of prior procedures.
Results: 440 colonoscopies were included in the data analysis. Procedures were stratified into three groups with reference to local geography: urban population (less than 15 minute commute), regional population (15 to 30 minute commute) and rural population (greater than 30 minute commute). The mean commute was 26.9km or 39.1 minutes. Analysis revealed that there was inadequate quality of bowel preparation in 11.8%, 12.4% and 11.8%, respectively.
Conclusion: About 1 in 10 patients have inadequate bowel preparation. In contrast to international literature, this study suggests there is no negative correlation between distance from endoscopy unit and quality of bowel preparation.
PP-01-032
Factor screening to improve the quality of bowel preparation: A prospective single-centre study from China
Yu Peng and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To screen the factors that affect the quality of bowel preparation and design a predictive model for improving the quality of bowel preparation.
Materials and Methods: A total of 1,000 patients who underwent electronic colonoscopy at the Digestive Endoscopy Centre of Xiangya Hospital Central South University were randomly selected. The model was built from 600 consecutive patients (development cohort) who were prospectively scheduled for colonoscopy between September and November 2023. The validation cohort included 400 patients who underwent colonoscopy between December 2023 and January 2024. Bowel preparation was classified as adequate or inadequate. Cleansing was evaluated using the Boston Bowel Preparation Scale (BBPS).
Results: In the development cohort, BBPS score was adequate in 355 patients (59.2%). In the multivariate analysis, dietary restriction (odds ratio [OR] 2.10, 95% confidence interval [CI] 1.49 –2.96), time interval from the last dose of preparation and colonoscopy (OR 0.40, 95% CI 0.33–0.48), and number of stools after bowel preparation (OR 1.65, 95% CI 1.28 – 2.12) were independent predictors for inadequate cleansing. The model built with these variables showed an area under the curve of 0.76 in the development cohort and 0.70 in the validation cohort. A cut-off of 1.50 predicted inadequate bowel preparation with a sensitivity, specificity, positive predictive value, and negative predictive value of 61.6%, 78.6%, 24.6%, and 94.2%, respectively in the development cohort, 61.2%, 75.1%, 27.3%, and 87.1%, respectively in the validation cohort.
Conclusion: The model could assist clinicians in predicting which patients are at high risk of inadequate bowel cleanliness.
PP-01-033
Factors that affect a patient's decision to undergo anesthetic colonoscopy: Prospective single-center study from China
Yu Peng and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To identify the factors that affect a patient’s decision to undergo anesthetic colonoscopy and establish a predictive model to decide whether sedation or analgesia is appropriate before the examination.
Materials and Methods: A total of 1,000 patients who underwent electronic colonoscopy in the Digestive Endoscopy Center of Xiangya Hospital at Central South University from September to December 2023 were randomly selected. Cleansing was evaluated using the Boston Bowel Preparation Scale (BBPS) by uniformly trained endoscopists. A questionnaire on the factors influencing the choice of anesthetic colonoscopy was completed by all patients after the examination, and a risk model for predicting pain during the colonoscopy was established.
Results: In the study, 439 patients chose anesthetic colonoscopy. In multivariate analysis, age (odds ratio [OR] 1.45, 95% confidence interval [CI] 1.08 –1.96), the pain tolerance threshold (OR 0.55, 95% CI 0.43 – 0.71), the degree of the worst pain (OR 2.45, 95% CI 1.66 – 3.60), and the duration of pain (OR 9.25, 95% CI 4.58 –18.70) were independent predictors affecting whether patients chose anesthetic colonoscopy. The model built with these variables showed an area under the curve of 0.833 for the cohort, with a sensitivity, specificity, positive predictive value, and negative predictive value of 86.5%, 62.4%, 87.6%, and 56.8%, respectively.
Conclusions: Patients who are older, have a low pain tolerance threshold, have previously experienced a high degree and longtime of pain in colonoscopy should choose anesthetic colonoscopy.
PP-01-034
Argon plasma coagulation in the management of chronic radiation proctitis at Jakarta tertiary referral hospital
Adiatmo Pratomo
Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
ABSTRACT
Introduction: Argon Plasma Coagulation (APC) is a non-tactile ablative therapy that helps to stop rectal bleeding in patients who have developed actinic proctitis after exposure to radiotherapy. Chronic Radiation Proctitis (CRP) is a complication that occurs in patients who receive radiation therapy for pelvic malignancies.
Objectives: This study evaluates the endoscopic features and clinical outcome of patients with Chronic Radiation Proctitis (CRP) after Argon Plasma Coagulation (APC) treatment at Digestive and Pancreatobilioary Endoscopy Department Cipto Mangunkusumo Hospital Jakarta.
Materials and Methods: The study is a descriptive cross sectional study with total sampling method from secondary data medical record that includes 81 patients who were diagnosed with CRP in the management of APC at Digestive and Pancreatobilioary Endoscopy Department Cipto Mangunkusumo Hospital Jakarta in 2020-2024.
Observation: The CRP in the management of APC were performed mostly in female patients (81,5%) and at aged range of 34 to 84 years old, with cervix cancer as the main cause of actinic proctitis (87,5%). Control of the bleeding after APC treatment was achieved in 98% of the cases.
Conclusion: APC treatment was successful in the treatment of CRP patient at Gastroenterology, Pancreatobiliary and Gastrointestinal Endoscopy Department, Cipto Mangunkusumo hospital, Jakarta.
Predictors of adenoma detection rate in colonoscopies for patients with positive fecal immunochemical test
R Rajesh, Kimwei Lim, Carlos Paolo Francisco, Christopher Khor Jen Lock and Ravishankar Asokkumar
Singapore General Hospital, SINGAPORE, Singapore
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Adenoma detection rate (ADR) is an established quality measure for colonoscopy. Professional societies recommend an overall ADR of 25% for screening colonoscopy and an overall ADR of 40% for patients who are faecal immunochemical test(FIT) positive. In this study, we evaluated the ADR in our patients who underwent FIT positive colonoscopy(FITC) and identified predictors that increased it.
Methods: We retrospectively reviewed the records of patients who underwent FITC between November 2016 to November 2020 at a tertiary medical centre in Singapore.
Results: We analysed 742 patients of which 382 (51%) underwent colonoscopy by gastroenterologists and 360 (49%) by non-gastroenterologists. The mean ±SD age was 66 ±8 years, and majority were females (52%). The mean Boston bowel preparation score was 6.6 ±1.6, the caecal intubation rate was 99%, and the withdrawal time was 14 ±11 minutes. The overall ADR, advanced adenoma detection rate(AADR), and adenoma per colonoscopy (APC) were 57%, 17%, and 1.24 respectively. We found the ADR was significantly higher for gastroenterologists than non-gastroenterologists (69% vs. 44%, p<0.001). Age (OR 1.03), endoscopists' years of experience (OR 2.82), performance of chromoendoscopy (OR 8.18), and withdrawal time (OR 1.22) were significantly predictive of higher ADR. The serrated adenoma and cancer detection rate was 2.8% and 4.6%.
Conclusion: FITC are held to more stringent quality metrics but this can be surpassed in routine clinical practise with appropriate training and experience. Specifically chromoendoscopy can be an important adjunct in improving adenoma detection in these patients.
PP-01-036
Efficacy of endoscopic direct diverticulitis therapy for acute diverticulitis
Jianzhen Ren, Silin Huang, Jun Cai, Bo Li, Guang Yang and Xiaolong Xian
Shenzhen University, South China Hospital, Shenzhen, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Acute diverticulitis is mainly treated through medication and surgical intervention. Digital single-operator cholangioscopy (DSOC) has proven effective for managing inflammations in natural conduits such as the bile duct, pancreatic duct, and appendix. This study aimed to investigate the diagnostic and therapeutic value of endoscopic direct diverticulitis therapy (EDDT) of acute diverticulitis.
Materials and Methods: We performed a retrospective study of patients who underwent EDDT from May 2023 to May 2024 at South China Hospital, Shenzhen University. In the study period, 6 patients with acute diverticulitis underwent EDDT using DSOC. The diagnosis of acute diverticulitis was confirmed by direct colonoscopy imaging and cholangioscope. Fecaliths were meticulously fragmented, extracted, and removed using a disposable basket following repeated lavages with metronidazole and sodium chloride, rendering the mucosa cleansed yet, without evidence of perforation. The success rate of DSOC-assisted EDDT, the procedure time, postoperative length of hospital stay, complications, and recurrence rate were recorded.
Results: Technical success rate was 100%, with high quality imaging of the diverticular cavity achieved using DSOC in all 6 patients. The mean procedure time was 95.17 (standard deviation [SD] =23.81 ) minutes. All patients experienced immediate relief from abdominal pain after the procedure. Mean postoperative hospitalization was 4.17 (SD=1.17) days. No recurrence occurred during 1–12 months of follow-up.
Conclusion: EDDT provided a feasible, safe and effective alternative approach for diagnosis and management of acute diverticulitis. This novel approach could reshape the management of acute diverticulitis, emphasizing the importance of technological integration in endoscopic practices.
PP-01-037
Inflammatory Cloacogenic Polyps in adolescents with chronic constipation. Can this be endoscopically managed?
Jayendra Seetharaman, Arul Premanand Lionel, Leenath Thomas, Sudipta Dhar Chowdury and Amit Kumar Dutta
Christian Medical College, Vellore, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Inflammatory Cloacogenic polyps/polyposis (ICP) are rare kind of polyps arising from anal transitional zone which can spread out in the distal rectum. ICP is one of the components of mucosal prolapse syndromes [1,2]. The diagnosis and management of ICP are poorly defined in literature, especially in the setting of constipation. We present two adolescents with constipation presented with ICP being endoscopically managed.
Case report: Case 1: 14-years old boy with four years of constipation with the feeling of ineffective evacuation presented with mass descending PR for three months. Colonoscopy showed multiple polypoidal lesions just above the anal verge. Endoscopic mucosal resection done (EMR) for large polypoidal lesion (4 cm) and hot snare polypectomy (HSP) in multiple settings done for the rest. Histopathology is consistent with ICP. 12 months follow-up showed polyp recurrence that was removed by HSP. Anorectal manometry (ARM) revealed type-1 dyssynergia. 6 months follow-up post biofeedback training did not show recurrence. Case 2: 16-years old boy presented with 10 years of constipation with mass descending PR for 2 months. HSP done in 3 sessions for the polypoidal lesions (0.5- 2cms) revealed ICP in histology (Figure 1). Bio-feedback sessions improved the symptoms after ARM showed type-1 dyssynergia. There was no pain or bleeding post-procedure in both the children
Conclusions: Polyps in the anal verge with inflammatory histology can be ICP. Endoscopic clearance in multiple sessions is required due to its minimal malignant potential. In the setting of constipation, dyssynergia needs to identified and treated to prevent recurrence.
PP-01-038
Improvement of colonoscopy insertion technique with the mikoto colonoscopy model: An international multicenter study protocol
1Department Of Endoscopic Diagnostics And Therapeutics, Saga University Hospital, Saga City, Japan;2Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga City, Japan;3Department of Gastroenterology and Nephrology, Tottori University Hospital, Yonago City, Japan;4Department of Endoscopy, The Jikei University School of Medicine, Tokyo, Japan;5Department of Gastroenterology, Saga-Ken Medical Centre Kosikan, Saga City, Japan;6Department of Gastroenterology, Saiseikai Karatsu Hospital, Karatsu City, Japan;7Department of Gastroenterology, Imari Arita Kyouritsu Hospital, Nishimatsuura-gun, Japan;8Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka City, Japan;9Department of Gastroenterology, Kitakyushu Municipal Medical Center, Kitakyushu City, Japan;10Department of Gastroenterology, National Hospital Organization Nagasaki Medical Center, Omura City, Japan;11Department of Gastroenterology, Sasebo City General Hospital, Sasebo City, Japan;12Department of Gastroenterology, Nagasaki Harbor Medical Center, Nagasaki City, Japan;13Department of Gastroenterology, Tottori Prefectural Central Hospital, Tottori City, Japan;14Department of Gastroenterology, Wakkanai City General Hospital, Wakkanai City, Japan;15Department of Internal Medicine, National Taiwan University Hospital, Taipei City, Taiwan;16Division of Endoscopy, Hokkaido University Hospital, Sapporo City, Japan;17Division of Gastroenterology and Hepatology, Mayo Clinic Arizona, Scottsdale, the United States of America;18Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Japan;19Department of Endoscopy and Gastroenterology, Nagasaki University Hospital, Nagasaki City, Japan;20Department of Clinical Research Center, Saga University Hospital, Saga City, Japan;21Advanced Medicine Innovation and Clinical Research Center, Tottori University Hospital, Yonago City, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: It is essential for beginning colonoscopists (trainees) to improve the quality of colonoscopy and accelerate their progress, and there are various training methods for this purpose. Furthermore, colonoscopy is more difficult than upper gastrointestinal endoscopy, and colonoscopy insertion training is important for the trainee. This prospective study (imikoto study) was designed with the hypothesis that training using the mikoto colonoscopy model will improve colonoscopy insertion technique in trainees.
Materials and Methods: This was an international, multicenter, randomized, controlled trial. Twelve trainees with limited colonoscopy experience (<50 cases) were eligible to participate in the study. One trainee per institution will be assumed, and institutions will be assigned to either the study group (mikoto training group) or the standard group (mikoto non-training group) with institution-based randomization (cluster randomization). The primary outcome will be cecal intubation time, and secondary outcomes will include adenoma detection rate (ADR), polyp detection rate (PDR), and ACE tool score. The sample size was calculated to be 35 cases each institution, 420 cases.
Results: The study is ongoing. (UMIN000054647)
Conclusions: Our study aims to provide evidence that training with the mikoto colonoscopy model improves colonoscopy insertion technique in trainees.
PP-01-039
Applying machine learning to predict bowel preparation adequacy in elderly patients for colonoscopy
Dalong Sun
Department Of Gastroenterology, Zhongshan Hospital, Fudan University(Xiamen Branch), Xiamen, China;Department Of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to develop and validate a machine learning model to predict bowel preparation adequacy in elderly patients before colonoscopy.
Materials and Methods: This study targeted elderly patients undergoing colonoscopy, employing the Boruta algorithm for feature selection. Predictive models were constructed using Logistic Regression (LR), Light Gradient Boosting Machines (LightGBM), Support Vector Machines (SVM), Decision Trees (DT), Random Forests (RF), and Extreme Gradient Boosting (XGBoost). Multiple performance metrics were used to compare predictive performance. The SHAP algorithm was utilized to rank feature importance and explain model outputs. A web-based application was developed using the Streamlit framework.
Results: The study included clinical data from 471 elderly patients with a 23.14% bowel preparation failure rate. The Boruta algorithm identified 7 key features. Among the models constructed, the SVM model performed optimally with an AUC of 0.895 (95% CI: 0.822-0.969), and accuracy, sensitivity, and specificity of 0.889, 0.739, and 0.932, respectively. SHAP analysis revealed significant impacts of these features on model decision processes, with contributions from factors like the last bowel movement being clear liquid, daily bowel movement conditions, and activity levels. The SVM model was transformed into a user-friendly web prediction tool to enhance its practical utility in clinical settings.
Conclusion: This study successfully developed and validated an interpretable machine learning model for predicting bowel preparation adequacy in elderly patients prior to colonoscopy, culminating in a practical web-based application. This model is expected to accurately identify high-risk patients, allowing for early interventions to significantly improve bowel preparation outcomes.
PP-01-040
Investigating the effects of music therapy on patient anxiety during colonoscopy
Aichi Medical University Hospital, 1-1 Karimata Yazako Nagakute, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Music therapy has shown promise in reducing anxiety during medical procedures. However, most studies focused on colonoscopies with sedation. We investigated the effects of music therapy on anxiety during colonoscopies performed by same-sex endoscopists without sedation.
Methods: This was a single-center, randomized controlled trial conducted at Aichi Medical University Hospital between August 2020 and October 2021. We enrolled 160 patients scheduled for colonoscopies and compared their clinical outcomes between music and non-music groups using the Stait-Trait Anxiety Inventory-Form JYZ (STAI) and the Visual Analog Scale.
Results: Overall, music therapy did not significantly reduce anxiety scores (STAI) compared to the non-music group (p=0.2674). However, female patients and those with a shorter insertion time (<10 minutes) in the music group showed significantly lower STAI scores compared to the non-music group (p=0.0275 and p=0.036, respectively). Additionally, the univariate analysis identified age, presence of polyps, and being a beginner endoscopist as potential risk factors for increased anxiety (p=0.004, 0.043, and 0.049, respectively). Multivariate analysis confirmed the presence of polyps (odds ratio=2.76, 95% confidence interval [CI] 1.2-6.39, p=0.017) and being a beginner endoscopist (odds ratio=2.98, 95%CI 1.21-7.38, p=0.018) were independent risk factors for higher anxiety.
Conclusion: While music therapy did not universally reduce anxiety during colonoscopy, it may be beneficial for female patients and those experiencing shorter procedures.
PP-01-041
Usefulness and safety of new ultrasmall-diameter colonoscope for cases with difficult insertion: A retrospective study
Division Of Gastroenterology And Hepatology, Department Of Medicine, Nihon University School Of Medicine, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colonoscopies are widely available, but there are cases where insertion can be difficult, even for experienced endoscopists. EC-760XP/L (FUJIFILM Corporation, Tokyo, Japan), a new ultrasmall-diameter long scope, may be useful in such cases. The objective of this study is to examine the effectiveness and safety of this new ultrasmall-diameter long scope.
Materials and Methods: This single-center retrospective study included 39 cases where colonoscope insertion was previously difficult. Endoscope insertion difficulties were defined as cases where cecal intubation could not be achieved by endoscopists of our hospital, or where the cecal intubation time was 15 minutes or longer despite an experienced endoscopist. The primary outcome was the cecum intubation rate using EC-760XP/L compared to a previous examination with a standard scope. The secondary outcomes included the cecal intubation time, sedative use rate, occurrence of adverse events, and pain experience.
Results: The standard scope achieved cecal intubation in 30 cases (76.9%), whereas EC-760XP/L reached the cecum in all cases (p < 0.01). Cecal intubation times with the EC-760XP/L (9.5 minutes) were significantly shorter compared to the standard scope (19 minutes) (p < 0.01). There was no significant difference in sedative use between the two groups. No adverse events associated with the examination were observed in either group. Pain was observed in 3 cases (8.3%) with the EC-760XP/L, significantly lower than the 22 cases (56.4%) with the standard scope (p < 0.01).
Conclusion: EC-760XP/L proved to be useful in cases where colonoscope insertion was difficult.
PP-01-043
Ultra early needle-knife fistulotomy versus conventional cannulation methods for biliary access during ERCP
João Fernandes1,2,3, Rita Ribeiro1, Alda Andrade1, Isabel Tarrio1, Tarcísio Araújo1, Patrício Costa2, Jorge Canena4,5 and Luís Lopes1,2
1ULS Alto Minho, Viana do Castelo, Portugal;2ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal;3ULS Cova da Beira, Covilhã, Portugal;4University Department of Gastroenterology - CUF Tejo Hospital - Nova Medical School, Lisbon, Portugal;5Amadora Sintra Hospital, Amadora, Portugal
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Primary needle-knife fistulotomy (NKF) was proposed as a superior method for biliary access compared to conventional cannulation methods (CCM), showing higher success rates in biliary cannulation and lower incidence of post-ERCP pancreatitis (PEP), even without specific PEP prevention measures. In clinical practice, endoscopists typically attempt CCM 2-3 times before considering alternative methods. We introduced the concept of ultra-early NKF (UE-NKF), involving one or two attempts lasting less than 2 minutes each before proceeding to NKF. We compared the outcomes of CCM versus UE-NKF in terms of biliary access success and adverse event rates.
Materials and Methods: A total of 373 patients were prospectively enrolled and randomly assigned to either the CCM or UE-NKF group based on their biliary access technique. We assessed PEP rates, overall adverse events, risk factors, procedural times, and successful cannulation rates between the two groups. All patients received PEP prevention measures.
Results: Both groups exhibited similar mean risk factors for PEP (CCM 1.33 ± 1.02; UE-NKF 1.31 ± 1.03). Mean cannulation times were 3 minutes and 13 seconds for CCM and 5 minutes and 10 seconds for UE-NKF. The cannulation success rate was 71.36% for CCM compared to 94.25% for UE-NKF (p < 0.001). The overall adverse event rate was 16.90% for CCM versus 13.33% for UE-NKF. PEP occurred in 9.15% of CCM cases and 6.06% of UE-NKF cases (p < 0.01).
Conclusion: UE-NKF demonstrates significantly higher rates of successful biliary access and lower complication rates, particularly PEP.
PP-01-044
Primary needle knife fistulotomy in a large population with different risks of post-ERCP pancreatitis
João Fernandes1,2,3, Henrique Coelho4, Rita Ribeiro1, Fábio Correia4, Alda Andrade1, Isabel Tarrio1, Tarcísio Araújo1, Gonçalo Alexandrino4, Luís Lourenço4,5, David Horta4,5, Patrício Costa2, Luís Lopes1,2 and Jorge Canena4,5
1ULS Alto Minho, Viana do Castelo, Portugal;2ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal;3ULS Cova da Beira, Covilhã, Portugal;4Amadora Sintra Hospital, Amadora, Portugal;5University Department of Gastroenterology - CUF Tejo Hospital - Nova Medical School, Lisboa, Portugal
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Needle-knife fistulotomy (NKF) as an initial method of cannulation has emerged as a technique associated with a high success rate and virtually no cases of post-ERCP pancreatitis, even in a population at high risk for PEP, without PEP prevention. The objective of this study was to assess the feasibility of performing primary NKF in a large group of consecutive patients with varying risks for PEP.
Materials and Methods: Multicenter prospective study including all consecutive ERCP patients who underwent primary NKF for biliary access. PEP prevention measures were employed in all cases. We evaluated the success rate, adverse event rate, and PEP rate in the overall population and in subgroups: low risk (0 risk factors for PEP) and high-risk (>1 risk factors). Procedural times and factors associated with PEP were also investigated.
Results: We included 302 patients (41 with low risk and 251 at high risk, with a mean risk factor of 1.5). The successful biliary cannulation rate was 97.4% (294/302). The mean time to achieve success was 4.54 ± 2.13 minutes. The adverse event rate was 2.6% (n=8), with a PEP rate of 1.65% (n=5) and no severe complications. PEP occurred only in patients with three or more risk factors (p < 0.001).
Conclusion: Primary NKF is feasible in consecutive patients with a high success rate and a low rate of PEP (although not zero). Having three or more risk factors for PEP increases the risk of PEP when using primary NKF.
ClinicalTrials.gov ID: NCT06452875
PP-01-044A
Recurrence of choledocholithiasis in patients submitted to cholangioscopy-guided lithotripsy-long-term follow-up
Rita Ribeiro1, Jorge Canena2,4, Tarcísio Araújo1 and Luís Lopes1,5
1ULS Alto Minho, Viana do Castelo;2University Department of Gastroenterology, CUF Tejo Hospital - Nova Medical;3School, Lisbon;4Amadora Sintra Hospital, Amadora;5ICVS – Life and Health Sciences Research Institute and ICVS/3B’s, Braga, Portugal
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Cholangioscopy-guided lithotripsy (CGL) is a safe and effective therapy in the treatment of difficult biliary stones. However, there is a lack of information about the long-term recurrence of choledocholithiasis after this technique. This study aimed to evaluate, in the long-term, the possible recurrence of choledocholithiasis in patients with and without gallbladder.
Materials and Methods: Single-tertiary center prospective study of all patients submitted to CGL between 2017 and 2023. The recurrence of choledocholithiasis, average time until its occurrence, therapies used during the recurrence, number and size of stones as well as factors associated with recurrence were evaluated.
Results: CGL was performed in 76 patients (51.3% female), 27 of whom had previously undergone cholecystectomy. The mean number of stones removed was 2, with a mean size of 19.4 mm and 92.1% achieved complete stone removal in the first session, with a 100% succeed rate after the second procedure. The mean follow-up time post CGL was 24 ± 18 months. There was recurrence of choledocholithiasis in 11/76 (14,5%) patients, of which 5 presented with acute cholangitis, with a mean time of 5.5 ± 6.6 months after CGL. In patients with recurrence, 10/11 (90.9%) were female and 8/11 (72.7%) had previous cholecystectomy (both P < 0.01 on multivariate analysis). The mean number of stones removed was 2.4 with a mean size of 21.1 mm. All recurrences were treated with conventional endoscopic techniques.
Conclusion: CGL is associated with a high success rate and with low rates of choledocholithiasis recurrence during follow-up. Previous cholecystectomy and female gender were independent predictors of choledocholithiasis recurrence.
PP-01-045
ERCP to the Rescue: From Iatrogenic Biloma to Recovery
Helen Engelina, Tirta Setiawan Tjokroprawiro, Hendra Koncoro and Maria Mayasari
Sint Carolus Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Percutaneous drainage is the treatment of choice for large bilomas. However, it may not always result in complete resolution in some patients. ERCP has emerged as both a diagnostic and therapeutic tool with a high clinical success rate
Case: A 30-year-old woman presented to the Emergency Unit with right upper abdominal pain, jaundice, and bloating. These symptoms began a month after a laparoscopic cholecystectomy, suggesting a probable iatrogenic bile duct injury leading to bilomas. Physical examination revealed a palpable mass and tenderness in the RUQ. Laboratory results showed anemia (Hb 8.2 g/dL) and elevated direct bilirubin (4.15 mg/dL). A CT scan revealed large cyst-like lesions near the liver and spleen. Percutaneous drainage was performed, which reduced the size but didn't completely resolve the issue, as confirmed by MRCP. ERCP revealed a bile leak (Hannover Classification type C1-C2) in the mid and proximal CBD. Subsequently, stent placement was performed to facilitate the healing of the leak. One day after the ERCP, the patient developed acute pancreatitis, which resolved with supportive treatment. The direct bilirubin level decreased to 1.47 mg/dL. She was discharged and reassessed a week later, reporting no complaints. An upper abdominal ultrasound showed significant reduction of fluid collection in the hepatic region.
Discussion: Percutaneous drainage of biloma is commonly used as an initial or supplementary approach to provide symptom relief. While ERCP becomes essential to address the underlying issue.
Conclusion: Combining percutaneous drainage with ERCP enables clinicians to manage bilomas more effectively thereby enhancing patient outcomes.
PP-01-046
Treatment Strategies for Pancreatic Stones and Appropriate Timing of Surgical Conversion
Yoshiyuki Harada, Arata Sakai and Yuzo Kodama
Kobe University, Kobe, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Minimally invasive endoscopic treatment is commonly used for symptomatic pancreatic stone disease. However, there is no clear consensus on when to transition to surgery if endoscopic procedures are unsuccessful. This study aims to determine the optimal timing for surgical intervention in cases of pancreatic stone disease.
Materials and Methods: We retrospectively reviewed the outcomes and prognoses of 122 patients who underwent endoscopic treatment for pancreatic stone disease at our hospital between November 2006 and October 2022.
Results: The median age of the patients was 61 years (range 6-84 years). Of the 122 patients, 77 (72%) underwent ESWL and 11 (9%) EHL, with 97 (80%) achieving complete removal of the pancreatic stones. Surgery was performed in 16 patients (13%). Among these, 2 patients were suspected of having pancreatic cancer and thus underwent surgery. Four patients who had surgery within one year of the initial treatment did not require pancreatectomy. In contrast, among those who had surgery more than one year after the initial treatment, 5 out of 10 patients required pancreatectomy (including 2 cases of PD). Moreover, one patient, who had portal vein obstruction, had difficulty with PD and subsequently underwent gastric jejunal bypass and bile duct jejunal anastomosis.
Conclusion: While endoscopic treatment for pancreatic stones generally yields favorable results, early surgical intervention should be considered for patients who are difficult to treat endoscopically.
PP-01-047
Ice water spray for the prevention of bleeding in precut sphincterotomy
Toshinobu Izumi and Mai Sorachi
NHO Himeji Medical Center, Himeji, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Although bleeding is a frequent complication of precut sphincterotomy, it should not be disregarded. Even a small amount of blood makes it difficult to obtain clear endoscopic view of the cutting site, resulting in likelihood of perforation. Ice water spray could cool down the duodenal papilla and cause vasoconstriction that is the same mechanism of hemostasis as epinephrine. We tried to prevent precut bleeding using ice water spray and evaluated efficacy and safety compared with conventional precut.
Materials and Methods: Between October 2019 and June 2024, 32 patients underwent precut sphincterotomy for biliary access after standard cannulation failed. 18 patients had conventional needle-knife precut by October 2021 and after that, 14 patients underwent ice water spray method. To cool down the papilla, 20ml of ice water was sprayed on the papilla through a needle-knife lumen immediately before starting precut. A small incision of precut was made repeatedly and 5ml of ice water was sprayed every interval of incisions.
Results: Success rate of biliary access in the ice water spray group and the conventional precut group was 93.0% and 94.4%, respectively. The bleeding rate that required hemostatic procedure including spray or injection of dilute solution of epinephrine and monopolar cautery was lower in the ice water spray group (6.70%) than the conventional precut group (22.2%). No complications of pancreatitis and perforation were found in both the groups.
Conclusion: Ice water spray during precut sphincterotomy might lower bleeding rate and contribute to performing precut sphincterotomy safely.
PP-01-048
Feasibility study of the usefulness using Bullet-type biliary metallic stents
Yuki Kawasaki
Showa University Koto Tosyosu Hospital, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The usefulness of biliary metallic stents for malignant biliary obstruction is already reported. Metallic stents are thought to provide longer stent patency due to their wider lumen compared to plastic stents. However, metallic stents, due to their wide lumen, increase the risk of retroreflux cholangitis. In the present study, the usefulness of a novel metallic stent (Niti-S○c, Biliary S-type Stent Bullet-type) was evaluated, which may reduce the risk of retroreflux cholangitis by tapering the distal end of the stent.
Materials and Methods: The technical and clinical success rate, adverse events, recurrent biliary obstruction (RBO) and time to RBO (TRBO) were evaluated in 15 patients who had Bullet-type metal stents placed between April 2023 and June 2024.
Results: The patient background was a median age of 84 years (50-93) and the primary disease was pancreatic cancer/biliary tract cancer/lymph node metastasis of other cancers 10/3/2. The technical and clinical success rates were 100%. No adverse events, including retroreflux cholangitis, were observed. RBO was observed in one patient (6.7%) and was due to migration. TRBO was 112 days (29-172).
Conclusion: Due to the short observation period, TRBO is shorter than for previously reported metallic stents, but the RBO rate is lower, and no adverse events have been observed. Bullet-type metallic stents may reduce the risk of retroreflux cholangitis better than conventional metallic stents.
PP-01-049
Risk factors of ascending cholangitis developing after endoscopic biliary stenting
Raja Taha Yaseen Khan and Hina Ismail and Abbas Ali Tasneem and Nasir Hassan Luck
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Ascending cholangitis is associated with increased morbidity and mortality in patients with history of ERCP. We aimed to identify the risk factors of ascending cholangitis following endoscopic biliary stent placement.
Materials and Methods: In this cross-sectional study, all the patients presenting at hepatobiliary clinic at least two weeks after biliary stenting were included in the study. While, the patients who subsequently found to have cholangitis due to causes e.g. stent occlusion, stent migration, sepsis, urosepsis, pneumosepsis and patients whose biliary drainage was achieved through percutaneous biliary drain (PTBD) placement were excluded. Presence or absence of ascending cholangitis was recorded. Univariate followed by multivariate cox regression analysis was performed to identify independent predictors of ascending cholangitis.
Results: A total of 128 patients were included in the study. Among them, 70(54.7%) were males. Most common indication for ERCP was CBD stricture noted in 87(68%) patients. Sixty six (51.6%) patients underwent two or more ERCP sessions. Papillotomy was performed in 69(53.9%) patients while sphincteroplasty was done in 38(29.7%) patients. Post ERCP, 53(41.4%) patients had a hospital stay of more than 3 days. Ascending cholangitis developed in 27(21%) patients. The presence of diabetes, prior history of multiple ERCP, papillotomy performed during ERCP and antibiotics given for short duration after ERCP were associated with increased risk of ascending cholangitis.
Conclusion: The risk factors associated with development of ascending cholangitis were recorded. Prolonged usage of antibiotics after ERCP in patients with these risk factors can decrease the risk of developing ascending cholangitis.
PP-01-050
Measurement of sliding force of covered metal stents for migration risk assessment in EUS-guided hepaticogastrostomy
1Department of Gastroenterology and Medicine, Fukuoka University, Fukuoka, Japan;2Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Migration of covered self-expandable metal stent (CSEMS) during endoscopic ultrasound-guided hepatogastrostomy (EUS-HGS) is a serious complication. The risk of migration is assumed to be influenced by the mechanical properties of the CSEMS, such as radial force (RF) and wire structure (braided or laser-cut types). However, the specific impact of these factors remains unclear. This experimental study aimed to identify the mechanical properties of the CSEMS that influence migration by measuring the sliding force (SF) generated when the CSEMS migrates from the gastric wall.
Materials and Methods: Seven types of CSEMS were evaluated, including four braided and three laser-cut types. An experimental model using porcine gastric walls and a universal testing machine was used to measure the SF of CSEMS. The CSEMSs were inserted through the porcine gastric wall, and traction was applied to simulate CSEMS migration. The maximum SF (SFmax) for each CSEMS was recorded. Additionally, the RF of each CSEMS was measured, and the correlation between SFmax and RF was analyzed.
Results: A very strong positive correlation (r>0.9) was found between SFmax and RF. The SFmax values of the laser-cut and braided type CSEMSs exhibited positive and negative residuals, respectively, when compared to the regression line predictions in scatter plots of SFmax and RF.
Conclusion: The RF of CSEMS is strongly correlated with SF, which together with the wire structure, influences the risk of migration during EUS-HGS.
PP-01-051
An unusual case of bowel obstruction following biliary stent migration
Chee Lim
SCUH, Sunshine Coast, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Endoscopic biliary stenting is a common bridging treatment for difficult to remove choledocholithiasis. 10% of stents migrate, but bowel obstruction from plastic stents is rare.
Case Description: We present a case of a 80-year-old male presenting with painful jaundice and fevers, on a background of cholelithiasis with intact gallbladder, and low anterior resection for rectal cancer. Workup revealed obstructive liver tests (bilirubin 220umol/L), gram negative bacteremia, and CT showing dilated common bile duct with large choledocholithiasis and an incidental 97x82mm cyst at the appendiceal tip.
During emergency endoscopic retrograde cholangiopancreatography (ERCP), a 7Fr x 5cm double pigtail stent (DPS) within a partially covered metal stent (SEMS) were deployed due to remnant choledocholithiasis and haemostasis. Despite an initial clinical improvement, patient developed worsening jaundice, vomiting and abdominal pain the next day. DPS was not seen endoscopically and on scout films, so SEMS was replaced with a 7Fr x 7cm DPS after biliary clearance. Follow up CT showed initial DPS lodged proximal to the appendiceal cyst with upstream obstruction. Laparotomy revealed widespread adhesions, and an ileocolic resection was performed to remove the cyst and DPS - histology showed a pT4apN0 appendiceal mucinous neoplasm.
Patient recovered, and 2 months later underwent laparoscopic cholecystectomy. Subsequent ERCP revealed a clear occlusion cholangiogram after retrieval of remaining DPS.
Discussion: While distal plastic biliary stent migration is commonly regarded as a fairly benign event, this case highlights the potential for significantly morbid complications in the setting of risk factors for bowel obstruction.
PP-01-052
CT Volumetry: A guide for optimal biliary drainage in unresectable malignant hilar biliary obstruction
Rishikesh Malokar1, Shubham Jain1, Manisha Joshi1, Prasanta Debnath1, Sanjay Chandnani1, Harsh Gandhi1, Jay Chudasama1, Sridhar Sundaram2 and Pravin Rathi1
1Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India;2TATA Memorial Hospital, Mumbai, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Abstract
Background and Aim: Outcomes of drainage in hilar biliary obstruction may depend on the volume of liver accessed and drained. The aim of this study was to determine the effect of draining more than 50% of liver volume on clinical success after intervention.
Material and methods: Advanced unresectable malignant hilar biliary obstruction (UMHBO) with Bismuth Corlette type II blocks and above were recruited prospectively. Patients underwent CT abdomen, and volumetric analysis was performed manually using TeraReconTM software. Patients were subjected to endoscopic or percutaneous drainage. The primary outcome was clinical success (a fall in baseline bilirubin of ≥50% on day 7), and secondary outcomes were complete drainage, cholangitis, reinterventions, and survival. Patients were categorized into those achieving≥ 50% and <50% drainage groups and outcomes were compared.
Results: A total of 196 UMHBO were screened, and 80 patients were analyzed after exclusion. Sixty-seven patients (83.75%) underwent ≥ 50% drainage. Clinical success was achieved in 47 (58.75%). Clinical success was significantly higher in the≥ 50% group (OR 3.411; 95% CI, 1.164-9.996; P =0.025), with lesser cholangitis (15%vs 26.3%; P=0.001), reinterventions (12.5%vs23.8%; P=0.001) with improved 90-day survival (58.8% vs 10%; P=0.013). On multivariate analysis, clinical success was a significant predictor against the development of cholangitis, and reinterventions and associated with higher complete drainage.
Conclusion: Preprocedural CT volumetry may guide the drainage approach with prospective validation of the concept of draining ≥50 % of liver volume. Higher volume drainage leads to better clinical outcomes, lesser cholangitis, reinterventions and 90-day survival.
PP-01-053
Pattern of bile cultures and antibiotic sensitivity tests in patients diagnosed with obstructive jaundice
Pratiwi Retnaningsih
Sebelas Maret University, Sukoharjo, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Abstract
Background: Biliary obstruction causes bacteriobilia and significant morbidity and high mortality, To indentify the characteristics of microbial isolates and from bile cultures taken from obstructive jaundice patients.
Methods: This was a prospective study conducted between January 2020 and December 2023 at Dr Moewardi Hospital, Surakarta, on 45 patients diagnosed with obstructive jaundice who an ERCP procedure electively. Bile samples were taken intraoperatively, then cultured and tested for antibiotic sensitivity.
Result: Sixty percent of patients diagnosed with obstructive jaundice who underwent ERCP were women with a ratio of (3:2). Thirty-two percent of patients were between 60 and 75 years old (elderly). The most common cause of obstructive jaundice is gallstones (43% of cases). The dominant isolated pathogenic bacteria in this study was Escherichia coli (40% of cases). This biliary pathogen is sensitive to Amikacin in 40% of cases and meropenem in 40% of cases. Finally, in all patients in this study, biliary pathogenic bacteria were found to be resistant to broad-spectrum antibiotics.
Conclusions: Microbiological analysis may become a diagnostic as it leads to more accurate selection of antibiotics for the treatment of cholangitis. Selection of empiric antibiotic therapy based on routine bile culture monitoring in patients at high risk of bacteriobilia will potentially help improve outcomes and optimize treatment of acute cholangitis, which is associated with a high mortality rate.
Keywords: Bile culture, Bacterial Sensitivity Test, Obstructive Jaundice
PP-01-054
Precut procedure using FlushKnife for endoscopic minor papilla sphincterotomy
Department Of Gastroenterology, Hyogo Prefectural Harima-himeji General Medical Center, Himeji, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Patients of pancreatic divisum may develop recurrent acute pancreatitis (RAP) due to dysfunction of the minor papilla. Therefore, endoscopic minor papilla sphincterotomy is sometimes performed for RAP patients diagnosed by pancreatic divisum. However, some cases are very challenging to insert not only catheter and papillotomy knife but even tip of guidewire to the accessory pancreatic duct (APD) through the poorly formed minor papilla.
Case Description: We report an 80s female case achieved the cannulation to the APD after the minor papilla precutting procedure using FlushKnife 1.5mm (FUJIFILM, Tokyo, Japan). After cannulation of the APD was obtained, an additional sphincterotomy (Clevercut papillotomy knife; Olympus, Tokyo, Japan) was performed and an endoscopic pancreatic stent was placed and removed after one week to prevent post-ERCP pancreatitis. RAP did not develop 24 months after EMPST.
Discussion: A precut procedure using a needle electric knife is sometimes performed by an expert endoscopist for difficult biliary cannulation through the main duodenal papilla; however, few cases have reported a precut procedure to cannulate the APD through the minor papilla. We usually perform the precut procedure for the main duodenal papilla in difficult biliary cannulation case using FlushKnife which is usually used for endoscopic submucosal dissection. A characteristic of FlushKnife is that the needle length is short. therefore, precutting procedures for the minor papilla can be performed safely without adverse events. The precut procedure for minor papilla using FlushKnife was useful and safe.
PP-01-055
Endoscopic retrograde cholangiopancreatography training model using a silicone simulator using a 3D printing technique
Suk Pyo Shin1, Kyong Joo Lee2 and Chang-Il Kwon Kwon1
1Cha Bundang Medical Center, Seongnam, South Korea;2Hallym University Dongtan Sacred Heart Hospital, Dongtan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: We aimed to determine whether endoscopy trainees acquired proficiency in ERCP techniques and assess any improvements in their skill levels from using this ERCP training model.
Materials and Methods: An ERCP training model was generated using 3D printing techniques, including five distinct interchangeable and transparent ampullar–common bile duct (CBD) modules. A prospective study using this model was conducted with ten trainees. The technical success rate and examination times for duodenoscope insertion and biliary cannulation were evaluated. In addition, the successful plastic-stent insertion rate and trainee satisfaction were measured.
Results: These training models simulated all steps of the ERCP procedure; fluoroscopic guidance was not required because of the transparency of the ampulla-CBD module. The success rates for duodenoscopy, cannulation, and plastic stent insertion were 94, 100, and 92%, respectively. The mean satisfaction scores for duodenoscope insertion, cannulation, and plastic stent insertion were 4.4, 4.7, and 4.6 on a 5-point scale, respectively. Five attempts decreased the insertion time (R = −0.591, P < 0.001) and cannulation time (R = −0.424, P = 0.002).
Conclusion: This ERCP-training silicon model is durable, simulates ERCP techniques easily, and helps trainees improve their ERCP techniques.
PP-01-056
Lemmel's syndrome: Case report of a successful endoscopic management
Dedy Sudrajat
Ciputra Hospital Citra Garden City Jakarta, Jakarta, Barat, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Lemmel's syndrome is a rare cause of obstructive jaundice caused by a periampullary duodenal diverticulum compressing common bile duct with resultant bile duct dilatation. We present a 56-year-old man came with worsening heartburn since 2 days before admission. The patient felt bloated, nauseous, and had decreased appetite. Physical examination showed epigastric tenderness and jaundice. Leucocytosis, elevated of transaminase enzymes, amilase, lipase, and bilirubine were detected. MRCP resulted bile stone in CBD, but ERCP found narrowing of distal CBD but didn't found any stone in CBD. A sphincterotomy was performed succesfully. Two month follow up, no recurrence jaundice was observed. Imaging is pivotal to diagnose Lemmel's syndrome accurately. Ultrasound could reveal biliary tract dilatation but cannot identify duodenal diverticula, which could be assessed by CT, MRI or ERCP. MRCP has very low sensitivity in the detection of duodenum diverticula, but is the method of choice to evaluate their relationship with the distal part of the CBD. The initial management is endoscopic retrograde cholangiopancreatography (ERCP), but in case of failure, interventional radiology and surgery can be an option.
PP-01-057
The influence of papilla morphology on biliary cannulation: A nationwide multicenter cohort study in Japan
Mamoru Takenaka and Koichi Fujita and Shujiro Yazumi and Toru Maruo and Kazuya Matsumoto and Masanori Asada and Hiroko Nebiki and Keiji Hanada and Toshiharu Ueki and Hirofumi Kawamoto
Kindai University, osaka-sayama, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and objectives: Although the impact of Vater papilla’s morphology on biliary cannulation has been reported, multicenter, multi-case analyses are limited. This study investigated the nationwide multicenter prospective cohort study in Japan.
Methods: The study enrolled 3647 patients with naïve Vater papilla undergoing selective biliary cannulation at 35 Japanese centers between April 2017 and March 2018.
The macroscopic appearance of the Vater papillae was classified as either separate or non-separate, and the length of the oral ridge, presence of diverticulum, and size of the papillae were also assessed to evaluate the impact of each factor on difficult biliary cannulation.
Result: The rate of the case in which cannulation times required longer than 5 minutes was significantly higher in the non-separated group (55.9(1657/2964) vs 46.0(314/683) %, P<.0001), the long oral protrusions group (58.7(612/1043) vs 52.2(1359/2604) %, P=0.0004) and the periampullary diverticulum non-exist group (55.9(1523/2724) vs 48.3(442/915), P<.0001).
The rate of the case with unexpected pancreatography was significantly higher in the non-separated group (41.3(1224/2964) vs 30.8(210/683) %, P<.0001), and the long oral protrusions group (42.9(447/1043) vs 37.9(987/2604) %, P=0.0062).
The rate of the case with unexpected guidewire insertion into the pancreatic duct was significantly higher in the non-separated group (35.5(1053/2964) vs 26.1(178/683) %, P<.0001), the long oral protrusions group (38.1(397/1043) vs 32.0(834/2604) %, P=0.0006) and the periampullary diverticulum non-exist group (35.0 (953/2724) vs 30.1 (275/915) %, P=0.0068).
Conclusion: In a nationwide multicenter analysis, Vater’s papilla’s morphology apparently influenced the difficulty of biliary cannulation.
PP-01-058
Effect of TXI assessments of Vater's papilla morphology on selective biliary cannulation: A multicenter study
Masayuki Kurimoto and Mamoru Takenaka and Yuki Tanisaka and Akihiko Yoshida and Hidekazu Tanaka and Tomohiro Fukunaga and Akashi Fujita and Masafumi Mizuide and Shomei Ryozawa and Masatoshi Kuso
Kindai University, osaka-sayama, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and Aims: Texture and color enhancement imaging (TXI) is considered superior to white light imaging (WLI) for performing endoscopic assessments of the orifice of the papilla of Vater. This improved ability to evaluate the orifice of the papilla of Vater using TXI may contribute to improved first-attempt cannulation (FAC) rates and reduced procedure times. This multicenter study aimed to determine whether the evaluation of the morphology of the papilla of Vater using TXI improves FAC rates and reduces biliary cannulation and procedure times.
Methods: Patients with naïve papilla of Vater who underwent selective biliary cannulation between June 2022 and October 2022 were retrospectively analyzed. The primary outcome of this study was the contribution of TXI to FAC.
Results: Biliary cannulation was performed in 57 and 56 patients using TXI and WLI, respectively. The biliary cannulation success rates with TXI and WLI were 98.3% and 98.2%, respectively (P=1.00). The FAC success rates with TXI and WLI were 45.6% and 25.0%, respectively (P=0.03). The time required for biliary cannulation and total procedure time of the FAC and non-FAC groups were 2 min and 12 min, respectively (P<0.0001) and 30.5 min and 44 min, respectively (P=0.0017). A multivariate linear regression analysis indicated that using TXI and separate-type papilla of Vater was associated with successful FAC.
PP-01-059
Development and evaluation of a self-expandable and easily removable biliary hydrogel stent
1Department of Gastroenterology and Hepatology, Tokyo Medical University, Tokyo, Japan;2Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: The study aimed to develop a novel biliary stent, the "Hydrogel Stent (HGS)," which combines the advantages of plastic stents (PS) and metallic stents (MS), offering self-expandability, long-term patency, removability, and replaceability. This was achieved using the swelling properties of polyvinyl alcohol (PVA) hydrogel as the driving force for expansion.
Methods: Building on previous research, we confirmed that the swelling and mechanical strength of PVA hydrogel can coexist. We further developed a high-strength HGS that initially resembles a PS and self-expands upon absorbing bile, akin to MS. We evaluated the stent's long-term patency and ease of removal through animal experiments.
Results: In its dry state, the cylindrical PVA gel exhibited flexibility similar to PS. Upon swelling, it expanded approximately 2.0 times in diameter and length, transitioning into an elastic material with a storage modulus and tear strength of 260 ± 20 kPa and 5.9 ± 0.5 N mm-¹, respectively. The stent demonstrated sufficient strength and flexibility for endoscopic delivery and could be removed endoscopically even after softening due to swelling. In vivo experiments on five pigs showed successful stent placement, with two cases of stent dislodgement and one case of successful endoscopic removal after 4 weeks. Post-removal evaluations revealed minimal bacterial adhesion.
Conclusion: The novel HGS developed using PVA offers promising features for endoscopic biliary stenting, including self-expandability, long-term patency, and easy removability.
PP-01-060
Significance of short guide wires in ercp-related procedures in Japan
Jun Ushio, Hisaki Kato, Yuki Kawasaki, Kazuya Sumi and Haruhiro Inoue
Showa University Koto-toyosu Hospital Digestive Disease Center, Koto-ku/Toyosu, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: In Japan and some Asian countries, long guide wire of 0.025 inch is often used for ERCP. On the other hand, in Western countries, short guide wire of 0.035 inch is mainly used, and therefore, there are differences in the devices used. In recent years, excellent instruments for ERCP have been developed from Asian countries, but they cannot be spread to Western countries without modification. Therefore, we examined whether short guidewires are appropriate for Japanese medicine.
Materials and Methods: The instruments were 0.035inch, 260cm Acrobat2TM guidewire (Cook Medical) and Fusion OMNI sphincterotome TM for bile duct cannulation, and various types of extraction balloon and monorail basket catheters were used for stone removal. Stone was removed in a single procedure, and the success rate, procedure time, and adverse events of the procedure were evaluated in 32 patients with common bile duct stones who had no previous papillary procedure were included in the study.
Results: All patients underwent successful bile duct cannulation, but 2 patients had difficulty with bile duct cannulation and underwent pancreatic sphincter pre-cutting. 7 patients had large stones larger than 10 mm, 25 patients had small stones, and 3 patients underwent lithotripsy. All patients were removed stones completely. Total procedure time averaged 8.7 minutes (6-19 minutes). There was one case of mild pancreatitis.
Conclusion: The success rate and safety of the short guide wire technique were satisfactory. The development of a device compatible with the short guide wire technique would be useful in Japanese medicine.
PP-01-061
Intraductal radiofrequency ablation for neoplasms of the major duodenal papilla with intraductal spread. first results
Ayubkhan Vagapov and Yury Starkov and Seda Dzhantukhanova and Rodion Zamolodchikov and Amina Badakhova
Vishnevsky National Medical Research Center for Surgery, Moscow, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The introduction of intraductal radiofrequency ablation into clinical practice provides new opportunities for minimally invasive treatment of patients with adenomas of the major duodenal papilla (MDP), including those with extensive extension to the bile ducts. The aim of our study was to evaluate the feasibility, effectiveness and safety of intraductal RFA in the treatment of patients with type III and IV of MDP adenomas with extensive intraductal spread of more than 10 mm.
Materials and Methods: From 2022 to 2023, 14 patients with adenomas of the MDP with extension to the CBD and/or МPD underwent intraductal radiofrequency ablation (Table 2). The extent of spread to the common bile duct ranged from 10 to 30 mm, to the main pancreatic duct - from 5 to 11 mm.
Results: Technical success was achieved in all observations. Complications after performing intraductal RFA were noted in 4 cases: in 2 cases, post-manipulation pancreatitis developed, and in another 2 cases, during control cholangioscopy, residual adenomatous growths were revealed, and therefore these patients required a repeat session of intraductal RFA. Technical implementation of stenting of the main pancreatic duct and common bile duct was achieved in all observations.
Conclusion: The use of intraductal radiofrequency ablation in the treatment of patients with adenomas of the MDP, characterized by extensive spread to the ducts, made it possible in all cases to ensure complete destruction of the intraductal component of the tumor with a lasting clinical effect without the need for highly traumatic surgical intervention.
PP-01-062
ERCP vs EUS for primary biliary drainage in malignant distal biliary obstruction: Systematic review andmeta-analysis
Praveen Reddy Vasepalli, Zaheer Nabi and Manu Tandon and Nageshwar Reddy
Asian Institute Of Gastroenterology, Hyderabad, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and Objectives: Endoscopic ultrasound-guided biliary drainage (EUS-BD) is a viable alternative in cases with failed ERCP transpapillary drainage (ERCP-TPD). This systematic review and meta-analysis aims to compare the efficacy and safety of EUS-BD and ERCP-TPD for primary biliary drainage in patients with distal malignant biliary obstruction (DMBO).
Methods: We searched Embase, PubMed, and Medline databases for studies comparing EUS-BD and ERCP-TPD in DMBO, from inception until September 2023. The primary endpoint was clinical success and secondary endpoints included technical success, procedure duration, and adverse events.
Results: Eight studies (815 patients, 56.1% male) were included in this analysis. Indications for biliary drainage were pancreatic carcinoma (75.1%), followed by cholangiocarcinoma (10.1%). Clinical success was comparable between EUS-BD and ERCP-TPD groups (OR 1.34, 95%CI 0.75 to 2.40; p=0.32). Technical success was similar between the two groups (OR 2.09, 95%CI 0.83 to 5.25; p=0.12). There was a trend towards fewer adverse events in the EUS-BD group (OR 0.65 [95%CI 0.40, 1.07]; p=0.09), with significantly lower odds of post-procedure pancreatitis (OR 0.17 [95%CI 0.06, 0.50]; p=0.001).
Conclusion: EUS-BD is comparable to ERCP for primary biliary drainage in DMBO patients with shorter procedural time and a significantly reduced risk of post-procedure pancreatitis.
Key words: Malignant biliary obstruction; ERCP; Metal stent; EUS
PP-01-063
Predictive factors for spontaneous passage of common bile duct stone
Wasuwit Wanchaitanawong1, Phichayut Phinyo2,3, Phuripong Kijdamrongthum1 and Nithi Thinrungroj1
1Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;2Center for Clinical Epidemiology and Clinical Statistics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;3Department of Biomedical Informatics and Clinical Epidemiology (BioCE), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To explore predicting factors associated with spontaneous passage of common bile duct stone (CBDS).
Materials and Methods: A retrospective observational cohort study of patients diagnosed with CBDS from January 2020 to December 2021 at Chiang Mai University Hospital was conducted. The confirmation of spontaneous passage of CBDS was performed by either endoscopic ultrasonography (EUS) or endoscopic retrograde cholangiopancreatography (ERCP).
Results: Of 172 patients with CBDS, 44 (25.6%) patients had spontaneous passage of CBDS, which was confirmed by ERCP in 34 patients and by EUS in 10 patients. In multivariable analysis, presentation with pancreatitis (RR 1.20, 95% CI 1.01 - 1.43), CBDS size ≤ 3.5 mm (RR 1.41, 95% CI 1.09 - 1.84), single CBDS (RR 1.21, 95% CI 1.08 - 1.35), distal location of CBDS (RR 1.11, 95% CI 1.01 - 1.22), and alkaline phosphatase (ALP) resolution (RR 1.13, 95% CI 1.00 - 1.28) were associated with the likelihood of spontaneous passage of CBDS. There was no significant difference in post-ERCP complications between patients who had spontaneous passage of CBDS and those with non-passage of CBDS (26.5% vs 19.8%, p = 0.479).
PP-01-064
Survival benefit of pancreatic stents in patients with pancreatic cancer: a retrospective cohort study
Weng Fai Wong1, Yu Ting Kuo1, Ming Lun Han1 and Hsiu Po Wang2
1Department of Integrated Diagnostics & Therapeutics, National Taiwan University Hospital, Taipei, Taiwan;2Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Malnutrition is common in patients with pancreatic ductal adenocarcinoma (PDAC), reducing their tolerance to anti-cancer treatments. Tumor-induced pancreatic duct obstruction blocks the outflow of pancreatic juice, causing maldigestion and poor nutritional status. We aimed to determine if pancreatic stents provide benefits in terms of survival and nutrition for patients with pancreatic cancer.
Materials and Methods: This retrospective study included patients with PDAC and obstructive jaundice who underwent endoscopic retrograde cholangiopancreatography (ERCP) for biliary stenting between 2007 and 2021 at a medical center in Taiwan. Pancreatic stents were placed following incidental pancreatic duct cannulation for the prevention of post ERCP pancreatitis (PEP), as determined by the endoscopists. Survival and nutritional status were compared between patients with and without pancreatic stents.
Results: Five hundred and sixty-two patients were enrolled, with 39 patients (7%) having pancreatic stents. The basic characteristics and the proportion of anti-cancer treatments were comparable between the two groups. The incidence of PEP was similar (stent vs. no stent, 5.1% vs. 2.1%, P = 0.227). However, patients with pancreatic stents demonstrated higher severity when pancreatitis occurred (BISAP score, median [IQR], 2.5 [2-3] vs. 1 [1-2], P = 0.045). The pancreatic stent group had shorter overall survival (median [IQR], 170 [95-306] vs. 217 [118-414] days, P = 0.077) and a lower one-year survival rate (HR 1.44, P = 0.048). The nutritional markers revealed no significant difference between the two groups.
Conclusion: Pancreatic stents provide no benefit in improving nutritional status or prolonging survival in patients with PDAC.
PP-01-065
Role of ampullary morphology in assessing difficult biliary cannulation and its impact on ERCP outcomes
Raghavendra Yarlagadda
American oncology institute/ Citizens hospitals, Hyderabad, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Biliary cannulation involves engaging of accessory with papillary orifice and traversing the intraduodenal part.Role of type of papillary orifice and intraduodenal part of CBD in the successful deep cannulation forms main objective of present study.
Material & Methods: Retrospective study of prospectively registered ERCPcases for biliary indications was performed.Papillae were classified-normal(Type I),small(Type II),bulging(Type IIIa),pendulous(Type IIIb),creased(Type IV) or peridiverticular(Type D)according to Mohamed etal(modified Haraldsson).Papillary orifice was classified according to Inomata classification,Separate type:isolated(papilla I),gyrate (papilla G)and septal type:Annular(Papilla A),Villous(Papilla V)and unstructured(papilla U).Difficult cannulation is defined as per ESGE guidelines.Any salvage techniques,adverse outcomes following the procedure were recorded according to ASGE-LEXICON.
Results: 72 naïve papilla from Oct2022-June2024 were included.Malignant etiology were 15,benign cases were 57(cbd stones50,distal CBD stricture4,bile leaks3).Periampullary diverticulum(type D)noted in 7 cases.Papillary type is not significantly associated with etiology.Type II and IIIb papillae and papillary orifice types A and U were associated with significantly longer cannulation times of 4.87(95%CI 2.18-7.32)and 3.11 (95% CI 1.36-7.24)minutes,3.82(95% CI 1.45 – 6.32),4.57(95% CI 3.13 – 7.24)respectively compared to regular papilla(type I) and separate papillary orifices.Type IIIb and U type orifice were associated with lower deep cannulation success.Precut was needed in 3 cases (type A and IIIb).PD stent is placed in 5 cases (septate type)due to inadvertent PD cannulation(typeU 4,typeA 1 and I,II,IV-2,2,1).No difference in cannulation failure between different papillae.Post ERCP pancreatitis in 5cases(typeU and typeII).No difference in the post-ERCP bleeding and infection rates between the different papillae.
Conclusions: Pendulous papilla(type IIIb)with unstructured(type U)were more difficult to cannulate compared to regular papilla.Identifying papilla type is useful because it may influence the success of cannulation.
PP-01-066
Effective combination therapy with full-covered metallic stent and self-assembling peptide for biliary bleeding
博士 Dan Zennyoji1, Takehiro Shimizu1, Aoi Kita1, Hiroko Hosaka1, Yoji Takeuchi2 and Toshio Uraoka1
1Department Of Gastroenterology And Hepatology, Gunma University Graduate School Of Medicine, Showa-machi, Maebashi, Gunma, Japan;2Department of Endoscopy and Endoscopic Surgery Gunma University Hospital, Showa-machi, Maebashi, Gunma, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Biliary bleeding caused by malignant tumors is difficult to manage, with endoscopic hemostasis using a full-covered metallic stent (FCMS) normally being the treatment of choice. PuraStat® (3-D Matrix, Tokyo), a self-assembling peptide, was developed as an absorbable local hemostatic agent and has been launched in Japan since December 2021 for gastrointestinal endoscopic procedures for oozing bleeding.
Case Report: A man in his 60s was diagnosed with acute cholangitis due to distal bile duct cancer (T3, N0, M1(HEP), Stage IV), and underwent ENBD. A 10Fr×70mm plastic stent was placed, followed by chemotherapy. Four months after the procedure, the plastic stent was replaced with an 8mm×60mm FCMS. Five months after the replacement, he was hospitalized for cholangitis. ERCP revealed the previous FCMS had dislodged, with continuous bleeding from the tumor. A 10mm×60mm FCMS was placed, however bleeding persisted from the gap between the papilla and the FCMS. PuraStat® (3ml) was applied around the FCMS, achieving hemostasis. After endoscopic hemostasis, no further bleeding was observed until the patient's death two months later
Discussion: FCMS alone failed to achieve hemostasis, but the application of PuraStat® effectively sealed the bleeding site. PuraStat® is easy to apply as it can be easily combined with other hemostasis methods.
PP-01-067
Predictive modeling of 30-day mortality in carbapenem-resistant enterobacterales sepsis Post-ERCP: A retrospective cohort study
1Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University, Hangzhou, China;2Key Laboratory of Integrated Traditional Chinese and Western Medicine for Biliary and Pancreatic Diseases of Zhejiang Province, Hangzhou, China;3Hangzhou Institute of Digestive Disease, Hangzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to examine the risk factors for 30-day mortality in patients with carbapenem-resistant Enterobacterales (CRE) sepsis following endoscopic retrograde cholangiopancreatography (ERCP) and to develop a nomogram for accurately predicting 30-day mortality risk.
Materials and Methods: Data from 235 patients who experienced post-ERCP CRE sepsis between January 2005 and December 2022 were analyzed. Variable selection was optimized via the least absolute shrinkage and selection operator (LASSO) regression model. Multivariate logistic regression analysis was then employed to develop a predictive model, which was evaluated in terms of discrimination, calibration, and clinical utility. Internal validation was achieved through bootstrapping.
Results: The nomogram included predictors such as age >80 years, ICU admission within 90 days prior to ERCP, hypoproteinemia, quick Pitt bacteremia score ≥2, post-ERCP pancreatitis, inappropriate empirical therapy, delayed definitive therapy, and short treatment duration (<10 days). The model demonstrated strong discrimination and calibration.
Conclusion: This study identified significant risk factors associated with 30-day mortality in patients with post-ERCP CRE sepsis and developed a nomogram to accurately predict this risk. This tool enables healthcare practitioners to provide personalized risk assessments and promptly administer appropriate therapies against CRE, thereby reducing mortality rates.
PP-01-068
Safety and necessity of pre-stenting endoscopic sphincterotomy in preventing severe pancreatitis
Toru Zuiki, Jun Oki, Takashi Ui, Ns Kaori Nakajima and Ns Mitsuko Sakairi
Yuki Hospital, Yuki, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Endoscopic retrograde biliary drainage (ERBD), involving plastic stent placement post-endoscopic retrograde cholangiopancreatography (ERCP), is crucial for acute suppurative cholangitis or obstructive jaundice due to tumors. However, post-ERCP pancreatitis following ERBD stenting presents a serious concern, attributed to pancreatic duct compression or obstruction by the stent tube, or heightened intrapancreatic duct pressure. In patients with severe pain and hyperamylasemia post-ERBD stenting, immediate stent tube removal prevents severe pancreatitis. Since 2020, procedural modifications included pre-ERBD stenting endoscopic sphincterotomy (EST) to mitigate pancreatic duct compression. The study aims to evaluate whether pre-stenting EST reduces severe post-ERCP pancreatitis.
Materials and Methods: This retrospective study involved 163 patients (91 men, 72 women) undergoing ERBD stenting for cholangitis or jaundice from January 2016 to May 2024. Outcomes were compared between 57 patients without EST and 106 with EST.
Results: No EST patients required stent tube removal, whereas three non-EST patients did. Among those requiring stent removal, post-procedure serum amylase levels exceeded 2000 IU/L in three non-EST patients, while none of the EST patients surpassed 1500 IU/L. Neither group experienced gastrointestinal perforation; two EST patients had manageable gastrointestinal bleeding. EST did not significantly affect pain or analgesic use, but no EST patients reported severe pain.
Conclusion: Pre-stenting EST appears effective in preventing severe post-ERCP pancreatitis without serious complications.
PP-01-069
Retractable robotic device for colorectal endoscopic submucosal dissection
Sang Hyun Kim and Hyuk Soon Choi
Korea University, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background/Aims: Appropriate tissue tension and clear visibility of the dissection area using traction are essential for effective and safe endoscopic submucosal dissection (ESD). In this study, we developed a retractable robot-assisted traction device and evaluated its performance in colorectal ESD.
Methods: An experienced endoscopist performed ESD 18 times on an ex vivo porcine colon using the robot and 18 times using the conventional method. The outcome measures were procedure time, dissection speed, procedure-related adverse events, and blind dissection rate.
Results: Thirty-six colonic lesions were resected from ex vivo porcine colon samples. The total procedure time was significantly shorter in robot-assisted ESD (RESD) than in conventional ESD (CESD) (20.1±4.1 minutes vs 34.3±8.3 minutes, p<0.05). The submucosal dissection speed was significantly faster in the RESD group than in the CESD group (36.8±9.2 mm2/min vs 18.1±4.7 mm2/min, p<0.05). The blind dissection rate was also significantly lower in the RESD group (12.8%±3.4% vs 35.1%±3.9%, p<0.05). In an in vivo porcine feasibility study, the robotic device was attached to a colonoscope and successfully inserted into the proximal colon without damaging the colonic wall, and ESD was successfully performed.
Conclusions: The dissection speed and safety profile improved significantly with the retractable RESD. Thus, our robotic device has the potential to provide simple, effective, and safe multidirectional traction during colonic ESD.
PP-01-070
Treatment Outcomes of ESD for Rectal pT1 Cancer: Single-center retrospective observational study
Yu Ebisawa, HIDEYUKI CHIBA, MASAKI KOGA, Akimichi Hayashi, Jun Arimoto, Hiroki Kuwabara and Michiko Nakaoka
OMORI RED CROSS HOSPITAL, Otaku, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Rectal cancer is suggested to have a higher potential for malignancy and recurrence compared to colon cancer. On the other hand, surgical treatment for lower rectal cancer (Rb cancer) is highly invasive, leading to an increase in diagnostic ESD for lesions suspected of submucosal invasion. However, the safety and efficacy of this approach remains unclear.
Material and Methods: A retrospective observational study was conducted on cases diagnosed with pT1 rectal cancer from April 2012 to March 2024, among 1,611 lesions treated with colorectal ESD at our hospital. The outcomes of ESD were compared between the Rb group and the non-Rb (Ra or Rs) group.
Results: Among 37 cases of rectal pT1 cancer, 16 were in the Rb group and 21 in the non-Rb group. Postoperative bleeding occurred in one case in the Rb group. Pathological evaluation showed the depth of invasion (pT1a/pT1b) as 4/12 in the Rb group and 9/12 in the non-Rb group. Only one case in the Rb group had a positive vertical margin. Among the 27 non-curative resections for rectal T1 cancer, 20 cases underwent additional surgical procedures, and lymph node metastasis was found in 4 cases (14.8%).
Conclusion: Rectal ESD was generally safe. However, for rectal T1 cancer as a whole, there were many non-curative resections. It is suggested that challenging ESD procedures were performed considering the location of the lesions. In this study, there was only one case with a positive vertical margin. However, for accurate pathological diagnosis, complete en bloc resection is expected.
PP-01-071
Using a novel hemostatic peptide solution to prevent bleeding after ESD of a gastric tumor
Kuniyo Gomi, Erika Yoshida, Misako Tohata and Masatsugu Nagahama
Showa University Fujigaoka Hospital, Yokohama, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Bleeding from an ESD ulcer occurs in 4.4% of post-ESD patients. We wondered if applying PuraStat® (PS) to post-ESD ulcers might reduce post-ESD bleeding. We investigated the preventive potential of PS, a hemostatic formulation, against bleeding in post-ESD gastric ulcers.
Materials and Methods: From May 2022 to March 2023, 101 patients (Group P) who underwent ESD for gastric diseases at our hospital received PS (2 mL) applied to their post-ESD ulcer. We retrospectively compared this group with a control group (Group C) comprising 297 patients who underwent ESD for gastric diseases at our hospital between April 2017 and March 2021. Post-ESD bleeding was the primary endpoint, while the secondary endpoints included the number of days from ESD to post-ESD bleeding and adverse events associated with PS administration.
Results: Post-ESD bleeding occurred in 6 (5.9%) (95%CI, 2.8-12.4) and 20 (6.7%) (95%CI, 4.4-10.2) patients in Groups P and C, respectively, with no significant between-group difference. Relative risk was 1.01 (95%CI, 0.95-1.07). No adverse events were observed with PS application. In addition, the median number of days between when ESD was performed and when post-ESD bleeding started was 2 (1–12) and 7.5 (1–14) days in Groups P and C, respectively, with no significant differ between the groups. The lesser curvature or anterior wall was the bleeding site in all 5 patients who experienced postoperative bleeding in Group P.
Conclusion: PS application is not associated with post-ESD bleeding. However, we infer that gravitational forces affect the effectiveness of applied PS.
PP-01-072
Post-ESD wounds closure using mantis clip: Experience with challenging cases
Kitakyushu Municipal Medical Center, Kitakyushu-city, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Endoscopic submucosal dissection (ESD) is commonly used for gastrointestinal tumors but carries risks such as bleeding and delayed perforation. Effective wound closure after ESD is crucial, yet conventional clips often struggle in areas with thick gastric mucosa and muscle layers. The Mantis clip, with its strong anchoring and gripping ability, may allow for complete wound closure using clips alone. We report a case where the Mantis clip was used in a challenging closure scenario.
Case: A male in his 60s underwent ESD for an 8 cm type 0-IIc lesion on the greater curvature of the antrum near the pylorus, achieving en bloc resection. Due to the large wound and high bleeding risk, closure was performed using Mantis clips. To prevent stricture formation, clips were placed to create a fold in the muscular layer, and complete closure was achieved with 11 Mantis clips and 9 Sure clips. No bleeding or perforation occurred, and the patient was discharged on day 8 post-treatment without complications. Follow-up endoscopy on day 85 showed complete scar formation with minor narrowing but smooth passage of the endoscope.
Discussion: The Mantis clip enables complete wound closure in cases where conventional methods are challenging. This technique shows promise in high-risk gastric cases and may prevent post-ESD complications. Further studies comparing its cost-effectiveness and preventive benefits are needed.
PP-01-073
Novel insulated tip knife combined with injector can decrease the burden of assistant during procedure
Sujin Kim and Jin Ook Jang
Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Procedure of endoscopic submucosal dissection (ESD) is composed of submucosal injection, precut, and dissection. One-step knife (OSK) is a novel knife that combines a knife for ESD and an injection needle into one sheath. In this study, we aimed to evaluate the safety, efficacy, endoscopist and assistant satisfaction of OSK during ESD.
Methods: We prospective collected medical records of consecutive patients with ESD using OSK from Sep. 2021 to Sep. 2023. We also checked the satisfaction of endoscopists and assistants using questionnaire (5-point scale; 1 very satisfactory, 2 satisfactory, 3 not so satisfactory, 4 dissatisfactory, and 5 very dissatisfactory) about overall procedure and submucosal injection.
Results: In the study period, a total of 203 patients were analyzed. The total procedure time was 11 min. The procedure time for each location was 14 min in the body, 10 min in antrum. Post-ESD bleeding and perforation rates were 3.9% and 1.0%. Regarding responses to the questionnaire, endoscopists rather than assistants yielded a very satisfactory trend. Rates of very satisfactory or satisfactory were 72% in endoscopists and 87% in assistants.
Conclusions: OSK can be a feasible knife for gastric ESD. Especially, it could increase the satisfaction of ESD procedure for endoscopists and assistants.
PP-01-074
The endoscopic resection for low grade gastric neuroendocrine tumors: A retrospective analysis
Wonjun Jang and Cheoul Woong Choi
yangsanpusan university, pusan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The recent surge in screening endoscopy has led to increased detection of gastric neuroendocrine tumors (G-NETs). The selection between radical surgical resection and local excision, including endoscopic resection, for the management of G-NETs remains controversial. Currently, endoscopic resection is recommended for G-NETs < 10 mm. We aimed to evaluate the feasibility of diagnostic endoscopic resection of G-NETs.
Methods: We retrospectively analyzed 31 patients diagnosed with grade 1 or 2 G-NETs at a single tertiary referral center between January 2009 and December 2023. Outcomes, including histopathology, complete resection, and metastasis rates, were analyzed.
Results: The mean follow-up period was 38.9 ± 38.4 months. The mean size of G-NET was 4.9 ± 3.4 mm, and most patients’ NETs were <10 mm (87.1%). The maximal NET diameter of NETs was 16 mm. During the study period, most NETs were grade 1 (type 1: 90.9%; type 3: 85.0%). R1 resection patients (19.4%) showed no evidence of metastasis during follow-up without additional surgical management. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Recurrent or multiple G-NETs were observed only in the patients with type 1 NETs (27.2%, 3/11). Modified endoscopic mucosal resection (EMR) (precut, ligation assisted, cap-assisted, and underwater), and endoscopic submucosal dissection (ESD) showed a 100% complete resection rate.
Conclusions: We observed that G-NETs 1 or 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection is recommended for gastric SETs ≤16 mm in size. Modified EMR or ESD is preferred to conventional EMR.
PP-01-075
Vonoprazan or proton-pump inhibitor for gastric endoscopic submucosal dissection in patients under antithrombotic therapy
1Division of endoscopy, Shizuoka Cancer Center, Sunto-gun Nagaizumi-cho, Japan;2Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya city, Japan;3Department of Gastroenterology, Japanese Red Cross Aichi Medical Center Nagoya Daiichi Hospital, Nagoya, Japan;4Department of Gastroenterology, Meitetsu Hospital, Nagoya city, Japan;5Department of Gastroenterology, Handa City Hospital, Handa city, Japan;6Department of Gastroenterology, Yamashita Hospital, Nagoya city, Japan;7Department of Gastroenterology, Ishikawa Prefectural Central Hospital, Kanazawa city, Japan;8Department of Gastroenterology, Kariya Toyota General Hospital, Kariya city, Japan;9Department of Gastroenterology, Konan Kosei Hospital, Konan city, Japan;10Department of Gastroenterology, Yokkaichi Municipal Hospital, Yokkaichi city, Japan;11Department of Endoscopy and Endoscopic Surgery, The University of Tokyo Hospital, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: The aim of this study was to evaluate the superiority of vonoprazan (VPZ) over proton pump inhibitor (PPI) in preventing delayed bleeding after gastric endoscopic submucosal dissection (ESD) in patients under antithrombotic therapy.
Materials and Methods: A multicenter, open-label, randomized controlled trial was conducted at 10 tertiary hospitals in Japan between September 2020 and October 2023. Patients with gastric neoplasms on antithrombotic agents scheduled for ESD were enrolled and randomly allocated in a 1:1 ratio to either the VPZ group (VPZ 20mg once a day) or the PPI group (Esomeprazole 20mg once a day). Antithrombotic agents were managed according to Japanese guidelines, and adjusted factors were institution and interruption of antithrombotic agents. The primary endpoint was the delayed bleeding rate within 8 weeks after ESD, evaluated using intention-to-treat analysis.
Results: A total of 125 patients were randomized, and 119 patients (median age 78 years, male/female 93/26) were included in this study (59 in the VPZ group and 60 in the PPI group). The delayed bleeding rates were 13.6% (8/59) in the VPZ group and 8.3% (5/60) in the PPI group, with no significant difference (P=0.36). Other adverse events included intraoperative perforation (two in the VPZ group, one in the PPI group), cerebral infarction (one in the VPZ group), drug rash (one in the PPI group), and aspiration pneumonia (one in the PPI group).
Conclusion: The present study did not demonstrate the superiority of VPZ over PPI in preventing delayed bleeding after gastric ESD in patients under antithrombotic therapy. (UMIN000040641/jRCTs041200045)
PP-01-076
A study of forceps resection for small esophageal squamous cell carcinoma
1Department of Gastroenterology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan;2Department of Pathology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: During surveillance endoscopy with iodine staining after endoscopic resection for superficial esophageal cancer, it is common to detect small esophageal squamous cell carcinomas (ESCCs). These ESCCs sometimes disappear upon biopsy as initial diagnosis, however, persistent cases pose challenges in determining the appropriate course of action. At our hospital, we occasionally perform excision using biopsy forceps for these ESCCs. This study aimed to evaluate the efficacy of forceps resection for small ESCCs.
Materials and Methods: From January 2019 to December 2023, we performed forceps resection on ESCC via endoscopic biopsy forceps at our hospital. We conducted a retrospective analysis on 23 lesions in 21 patients who were followed for more than 6 months.
Results: The median age was 74 years (range 49-96), with a male to female ratio of 19:4. The median lesion diameter before forceps resection was 4 mm (range 2-7). All patients were diagnosed with squamous cell carcinoma on initial biopsy and had endoscopically diagnosed as cT1a-EP/LPM; none showed signs of deep invasion. The median number of forceps samples taken per lesion was 2 (range 1-4), and the median follow-up period by endoscopy was 19 months (range 6-46). No instances of local recurrence were observed.
Conclusion: Short-term outcomes of forceps resection for small ESCCs were promising. Although further research is needed, forceps resection appears to be an effective, immediate, and minimally invasive treatment option that does not require hospitalization.
PP-01-077
Effective training methods for colorectal endoscopic submucosal dissection (ESD) using outside video and strategy note
Mikio Kobayashi, Hideyuki Chiba, Masaki Koga, Akimichi Hayashi, Yu Ebisawa, Jun Arimoto, Hiroki Kuwabara and Michiko Nakaoka
Omori Red Cross Hospital, Ota-ku, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: When a trainee performs ESD at our institution, the trainee is generally instructed under the backup of the supervisor, emphasizing on angle manipulation and stability of the scope operation. We not only take inside but also outside video of endoscopic manipulations during ESD, including the voice of the supervisor providing advice and the sounds of the high-frequency device. After synchronizing inside and outside video, two screens video of the case is made and the trainee can take detailed feedback during review. Before ESD, the trainee also simulates the target lesion in as much detail as possible and prepare strategy note.
Materials and Methods: 1498 cases of colorectal ESD were performed at our institution from April 2012 to April 2024, and all 1495 cases had en bloc resection except for 3 cases in which treatment was interrupted.
Results: The resected lesion size was 30 mm (median: 10 mm - 220 mm), and the procedure time was 29 minutes (median: 3-600). 740 cases were performed by 9 trainees (started with ≥30 gastric ESD cases and up to <100 colorectal ESD cases). Trainee's results were excellent with a lesion size of 28 mm (median: 10 mm - 150 mm), a treatment time of 33 minutes (median 7-480), and a self-completion rate of 89.6%.
Conclusion: The training methods for colorectal ESD at our institution using a combination of outside video and strategy note were safe and effective.
PP-01-078
Efficasy of purastat after endoscopic submucosal dissection for gastric neoplasm.
Sanshiro Kobayashi and Yuu Takahashi and Tomomitsu Tahara and Naohiro Nakamura and Takuya Shijimaya and Yasushi Matsumoto and Makoto Naganuma
Kansai Medical University, Osaka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Post-ESD bleeding for gastric neoplasm is occurred in about 5%.
Purastat is a synthetic petptide-based hemostatic agent. Upon contact with acid or bloo, d, it rapidly forms nanofibers and gels, creating p physical barrier at the bleeding site.
Therefore, we examined the efficacy of Purastat on post-ESD Bleeding.
Matrials and Methods: We retrospectively compared Purastat group which applied to the post-ESD ulcer of stomach at our hospital from September 2023 to February 2024 and thrombin group from September 2022 to February 2023.
We defined Post-ESD bleeding cases as decreasing Hb of 2 g/dl or more.
Results: Purastat/Thrombin group were 63/66 cases. Age 75 (40-90)/75.5 (55-96) years old.
Procedure time 60 (10-298)/53.5 (10-363) minutes. Tumor resection size 824.3 (175.8-4239)/686.8 (211.6 -5004.3) cm2. Under oral anticoagulantion 18/16 cases.
Diabetes mellitus 21/10 cases. Median tumor resection size tended to be larger in the PuraStat group. There were significantly more cases of diabetes in the Purestat group. PuraStat group had 2 cases of post-ESD bleeding. One case took aspirin. 4 cases were seen in the thrombin group, one case took apixaban and the other case took clopidogrel.
Conclusion: Despite the larger resection size and significantly more diabetic patients, there was a trend toward less post-ESD bleeding in the PuraStat group.
PP-01-079
Management of intraoperative perforation during colorectal underwater endoscopic submucosal dissection.
1Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;2Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan;3Center for Diagnostic and Therapeutic Endoscopy, Keio University School of Medicine, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The underwater technique facilitates the creation of a field of view in colorectal endoscopic submucosal dissection (ESD). Although there are concerns about abdominal pollution with intestinal contents when intraoperative perforation occurs, little is known about the actual clinical course of perforation during colorectal underwater ESD (UESD) in the real world. We aimed to clarify the clinical course of patients with perforations during colorectal UESD.
Materials and Methods: This was a retrospective observational study. We reviewed cases with intraoperative perforations during colorectal UESD in our institution between June 2020 and May 2024. We evaluated the clinical characteristics, ESD outcomes, management of perforation, and clinical course after ESD.
Results: Among 534 cases of colorectal UESD, intraoperative perforation occurred in 25 cases (4.7%). The median age was 64 [range, 43−86], the median lesion size was 25 mm [range, 12−80], and the most common location was the ascending colon (30%). En-bloc resection was achieved in 24 cases (96%). Only the perforation site was closed with clips in 15 cases (60%), while the entire wound was completely closed with clips in the remaining cases. After the procedure, fever (>37.5°C) and localized abdominal pain were seen in 8 cases (29%) and 11 cases (46%), respectively. Antibiotics were administered in 21 cases (84%). The median length of hospital stay was 3 days [range,3-9] after colorectal UESD, and there were no cases requiring emergent surgery.
Conclusion: Intraoperative perforations during colorectal UESD could be controlled only by conservative treatment.
PP-01-080
Comparisons cold snare and hot snare endoscopic mucosal resection for intermediate sized colorectal polyps
Chang Kyo Oh1, Young Wook Cho2, Jin Bae Kim1 and Young-Seok Cho2
1Hallym University Kangnam Sacred Heart Hospital, Hallym University College Of Medicine, Seoul, South Korea;2Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background/Aims: Endoscopic mucosal resection (EMR) is a standard method for the resection of Intermediate sized colorectal polyps. However, several studies are currently conducting on the feasibility of cold snare EMR (CS-EMR) for polyps larger than 10 mm based on the safety of cold resection. Even aside from the cauterization effect, EMR is still widely used because of the benefit of submucosal injection (making the margin of the lesion easier visible and manipulating the lesion into a form that is easier to remove). We aimed to assess the efficacy of CS-EMR, compared with hot snare EMR (HS-EMR), for 10-15 mm sized colorectal polyps.
Methods: 10-15 mm sized colorectal polyps were randomly allocated to either the CS-EMR group or the HS-EMR group. Primary outcome was recurrence and residual adenoma (RRA) rate.
Results: A total of 70 and 70 polyps were resected using CS-EMR or HS-EMR, respectively. In the intention-to-treat population, the RRA rate was 1.4% in the CS-EMR group and 1.4% in the HS-EMR group (p=1.000). En bloc resection rate for the CS-EMR and HS-EMR group was 72.9% vs 91.4% (p=0.004) and R0 resection rate was 47.1% vs 78.6% (p=0.001), respectively. Delayed bleeding was 2.9% vs 4.3% (P =0.681) in CS-EMR group and in HS-EMR group, respectively.
Conclusion: CS-EMR was non-inferior to HS-EMR for the RAA of 10-15 mm sized colorectal polyps. CS-EMR can be considered one of the preferred methods for the removal of 10-15 mm sized benign colorectal polyps.
PP-01-081
Usefulness of strip biopsy for small gastric neoplasia
Nihon University School Of Medicine, Itabashi-ku, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Endoscopic submucosal dissection (ESD) is a standard treatment for early gastric cancer without lymph node metastasis, but is technically challenging, particularly for lesions in the upper (U) and middle (M) third of the stomach. Strip biopsy, a simpler endoscopic mucosal resection (EMR) method, is used for small gastric neoplasia. This study investigates the efficacy and safety of strip biopsy for small gastric neoplasia.
Materials and Methods: We retrospectively compared the therapeutic outcomes in 54 patients who underwent ESD (ESD group) and 6 patients who underwent strip biopsy (strip biopsy group) for small gastric lesions (≤15 mm) at our hospital from April 2022 to March 2024.
Results: The median lesion size was 10 (2-15) mm in the ESD group vs. 4.5 (2-15) mm in the strip biopsy group (p = 0.04). The median resected specimen size was 29.5 (17-40) mm vs. 11 (9-23) mm (p < 0.01). The lesion in U and M/lower stomach was 33/21 vs. 5/1 (p = 0.40). The median procedure time was 43 (10-120) vs. 6.5 (3-8) minutes (p < 0.01). Both groups had a 100% en bloc resection rate. The R0 resection rate was 96.3% vs. 100%, with no significant difference. There were 3 cases of delayed perforation (5.6%) and 0 cases of delayed bleeding in the ESD group, while the strip biopsy group had no such cases.
Conclusion: Strip biopsy is a useful treatment for small gastric lesions, offering a shorter procedure time without inferior treatment outcomes compared to ESD.
PP-01-082
Effective closure of large duodenal esd defect using mantis clip with suction: Case report
Department of gastroenterology, Kitakyushu Municipal Medical Center, Kitakyushu City, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Endoscopic submucosal dissection (ESD) for duodenal tumors is technically challenging and high-risk. Secure closure of the mucosal defect is crucial to prevent delayed perforation. The Mantis clip (Boston Scientific) enables closure of large post-ESD defects with clips alone.
Case: A 70-year-old male underwent ESD for a duodenal tumor. An 8 cm mucosal defect was closed using Mantis clips. The closure technique involved:1. Water immersion to prevent defect expansion; 2. Long hood attachment with suction; 3. Tissue grasping and inversion with Mantis clips; 4. Closure using 8 Mantis clips and 5 Sure Clips; No postoperative complications occurred. The patient was discharged after one week.
Discussion: The Mantis clip offers advantages over conventional clips:
1. Strong grasping power;
2. Large tissue retention capacity;
3. Minimal positional shift;
This technique allows simple procedure completion using clips alone. Combining suction with a long hood enables effective closure of large mucosal defects. Further studies are needed to evaluate its effectiveness in preventing postoperative complications.
PP-01-083
Japanese Gastric Cancer Treatment Guidelines can be safely applied for endoscopic resection in the west
Edward Young1,2, Louisa Edwards2,3, Andrew Ruszkiewicz1,4 and Rajvinder Singh1,2
1Lyell McEwin Hospital, Northern Adelaide Local Health Network, Adelaide, Australia;2Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia;3Royal Adelaide Hospital, Central Adelaide Local Health Network, Adelaide, Australia;4SA Pathology, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: There is considerable disparity in the incidence of gastric cancer between eastern and western countries, with many eastern countries now participating in nationwide gastric cancer screening programs. This has led to the development of risk-stratification models to identify appropriate lesions for endoscopic resection, included in the Japanese Gastric Cancer Treatment Guidelines (2021). We sought to assess whether these guidelines can be safely applied in a western population.
Materials and Methods: Data was retrospectively recorded for all patients who underwent any form of gastrectomy in four Australian Public Hospitals between 2000 and 2021. Demographic data, lesion characteristics (size, differentiation, invasion depth, lymphovascular invasion and ulceration) as well as the presence and number of lymph node metastases was recorded.
Results: A total of 1,465 patients were included, 558 of whom underwent gastrectomy for gastric adenocarcinoma (median age 70, 64.2% male). Of these, only 18.4% (n=101, CI 15.4-21.9%) were T1 lesions. Based on the Japanese Gastric Cancer Treatment Guidelines, 11.5% (n=64, CI 9.1-14.4%) met absolute criteria for endoscopic resection, with 7.8% (n=5, CI 3.4-17%) having positive lymph nodes at gastrectomy. According to the eCura system, 9.9% (n=55, CI 7.6-12.6%) would have been considered eCura A or B based on their histology, with none of these having positive lymph nodes at gastrectomy.
Conclusion: The eCura system for endoscopic curability could have been safely applied in this western population. Even in western countries, patients with early gastric cancer meeting Japanese guidelines for endoscopic resection should where possible undergo en bloc endoscopic resection.
PP-01-084
EG-840TP with a dedicated hood can safely resect an early esophageal cancer with esophageal stricture
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The EG-840TP (Fujifilm Co, Tokyo, Japan) has a tip diameter of 7.9 mm, which is thinner than conventional therapeutic endoscopes. We have used it for endoscopic procedures with a dedicated tapered hood with a 4 mm tip. We present a case in which ESD was successfully completed using these devices in a patient with early esophageal cancer and cervical esophageal stricture.
Case report: The patient is a 75-year-old man with cervical esophageal stricture caused by chemoradiotherapy for the right pyriform sinus carcinoma. An EGD was performed to screen for metachronous cancer, using an ultrathin 5.8 mm tip diameter endoscope due to the stricture. There was a semi-circumferential 0-IIb lesion in the mid-thoracic esophagus, which was suspicious for squamous cell carcinoma on biopsy. We planned ESD for this lesion and the EG-840TP was inserted with the dedicated hood. The tapered side of the hood wedged into the stricture and bougie dilatation was performed without problems. We were able to reach the lesion and complete the resection by ESD. The pathology results showed a curative resection.
Discussion: Endoscopic treatment of a lesion located beyond a stricture requires ingenuity. Balloon dilation of the stricture carries a risk of bleeding and perforation. Bleeding makes ESD difficult due to poor visualization. Although it is possible to perform the procedure with an ultrathin endoscope, the small channel diameter limits the devices available. The approach using the thin therapeutic endoscope and the dedicated hood is useful for lesions on the deeper side of a stricture.
PP-01-085
Conventional endoscopic mucosal resection as a cost-effective and potent option for diminutive rectal neuroendocrine tumors
Dong Jun Oh
Dongguk University Ilsan Hospital, Goyang, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Endoscopic mucosal resection (EMR) is being conducted for the treatment of small (≤10mm) rectal neuroendocrine tumors (NETs). Several studies suggest utilizing modified EMR to achieve complete resection. However, in resource-limited settings, modified EMR may be challenging to perform. And few studies have compared conventional EMR and modified EMR in the treatment of diminutive (≤5mm) rectal NETs. This study aims to compare the clinical outcome and cost-effectiveness of conventional EMR (cEMR) and cap assisted EMR (EMR-C) for diminutive rectal NETs.
Materials and Methods: This single-center retrospective study included patients who underwent endoscopic treatment for diminutive rectal NETs between March 2022 and March 2024. All EMR procedures were performed by experienced endoscopists.
Results: A total of 68 patients were enrolled. Seventeen patients underwent cEMR, while 48 patients underwent EMR-C. The mean histologic size of NETs was 3.5 ± 1.2 mm in the cEMR and 3.4 ± 1.4 mm in the EMR-C. The complete resection rate was 94.1% (16/17) in the cEMR and 93.8% (45/48) in the EMR-C, with no significant difference (p = 0.999). There were 3 cases of delayed bleeding in the EMR-C, while no complications were observed in the cEMR (p = 0.702). The mean hospitalization period for patients who underwent EMR-C was 2.1 days, while all EMR patients were managed on an outpatient setting.
Conclusion: Based on the available endoscopic equipment and expertise, cEMR can be considered a completely resectable and cost-effective treatment option for diminutive rectal NETs. Further multicenter prospective studies are needed.
PP-01-086
Argon plasma coagulation versus endoscopic resection to treat gastric adenoma: A systematic review and meta-analysis
Jin Hwa Park1, Jae Gon Lee2, Sang Pyo Lee1 and Kang Nyeong Lee1
1Hanyang University, Seoul, South Korea;2Hallym University, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Endoscopic resection (ER) is regarded as the best treatment for gastric adenoma. Argon plasma coagulation (APC) is also widely used to treat gastric adenomas, but its efficacy and safety have not been fully established. We performed a systematic review and meta-analysis to determine the efficacy and safety of APC compared with ER in treating gastric adenoma.
Materials and Methods: PubMed, EMBASE, and the Cochrane Library were searched up to April 2024. All studies that evaluated the clinical outcomes of APC or ER for treating gastric adenomas were included. Outcomes included local recurrence rate, procedure time, length of hospital stay, and complications.
Results: A total of 7 studies were included, of which 3 were retrospective single-arm studies that reported only outcomes of APC, and 4 were retrospective studies that compared the outcomes of APC vs ER. APC showed a higher local recurrence rate in treating gastric adenoma than ER (risk ratio [RR] 4.378, 95% CI 1.995-9.607), but resulted in shorter procedure times (MD -45.228, 95% CI -49.436 to -41.021), shorter hospital stays (MD -2.684, 95% CI -2.932 to -2.437), and fewer complications (RR 0.329, 95% CI 0.124 to 0.869).
Conclusions: APC was associated with a higher local recurrence rate, but a lower risk of complications than ER. APC can be considered an alternative to ER in treating gastric adenomas.
PP-01-087
Cold versus hot endoscopic mucosal resection of large non-pedunculated colorectal polyps: A meta-analysis
Federico Iv Peralta, Nikko Theodore Raymundo and Enrik John Aguila
St. Luke's Medical Center - Global City, Taguig, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Large non-pedunculated colorectal polyps (>20mm) can be effectively managed with hot snare EMR (H-EMR); however, electrocautery-related complications may incur substantial morbidity. It has been suggested that cold snare EMR (C-EMR) may be better than H-EMR because of fewer adverse events. This meta-analysis aims to compare the outcomes of H-EMR and C-EMR.
Materials and Methods: Relevant studies were identified through systematic searches of PubMed, MEDLINE, Cochrane, and Google Scholar databases. Out of 173, four eligible studies (2 randomized controlled trials and 2 retrospective studies) were included in the analysis. Quality assessment was performed using the Cochrane Risk of Bias tool for the RCTs and the Newcastle-Ottawa scale for the retrospective studies.
Results: A total of 1293 large non-pedunculated colorectal polyps resected using EMR were included (367 C-EMR vs. 906 H-EMR). There is no statistically significant difference in the technical success rate of C-EMR compared to H-EMR (OR 2.11, 95% CI [0.14-31]; I²=67%). Additionally, there is no significant difference in the risk of perforation between the two methods (OR 0.23, 95% CI [0.04-1.30]; I²=0%). H-EMR has a higher risk of delayed bleeding compared to C-EMR (OR 0.2, 95% CI [0.07-0.56]; I²=0%). In contrast, C-EMR has been shown to have a higher rate of polyp recurrence compared to H-EMR (OR 2.56, 95% CI [1.40-4.70]; I²=43%).
Conclusion: C-EMR is non-inferior to H-EMR in the resection of large nonpedunculated colonic polyp. C-EMR appears considerably safer with a lower risk of delayed bleeding than H-EMR; however, at the cost of a higher polyp recurrence rate.
PP-01-088
Pancreatic toxoplasmosis based on endoscopic ultrasound evaluation: A rare case report
Cosmas Rinaldi A Lesmana1,2, Randy Adiwinata1 and Jennifer Simsca1
1Gastrointestinal Cancer Center, MRCCC Siloam Hospital Semanggi, Jakarta, Indonesia;2Division of Hepatobiliary, Department of Internal Medicine, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine Universitas Indonesia, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Toxoplasmosis can present as systemic disease affecting many organs, especially in immunocompromised patients. Most cases of toxoplasmosis present as encephalitis, while extracerebral toxoplasmosis is rare, particularly in gastrointestinal tract. Here we reported 48-year-old HIV infected male patients with toxoplasmosis manifesting as pancreatic nodule, chronic pancreatitis and encephalitis
Case description: A 48-year-old male HIV patient was referred to our hospital for evaluation of pancreatic nodules. He had been hospitalized in previous hospital due to hemiparesis and dizziness. Brain MRI with contrast revealed solid-occupying-lesion in cerebellum with surrounding edema. PET scan indicated focal metabolic sign in the head and body of pancreas suggesting for primary pancreatic tumor. Lipase was elevated below 2 times of upper-limit-normal. Amylase, Ca 19-9, and CEA was normal. We performed endoscopic ultrasound (EUS) and we found multiple hypoechoic nodules in pancreatic head and body. EUS fine needle biopsy (FNB) using 22-G acquire needle (Boston Scientific, USA) was then performed for further evaluation. Histopathology examination showed chronic pancreatitis, no malignant cell, and bradyzoite-containing tissue cysts suggestive of toxoplasmosis. He was treated for toxoplasmosis by infectious disease specialist.
Discussion: Pancreatic Toxoplasmosis is rarely reported due to its uncommon occurrence, even in HIV patients. Ahuja et al. reported a case of necrotizing pancreatitis and multisystem organ failure associated with toxoplasmosis in AIDS patient. Toxoplasma may directly invade pancreatic organ and induce inflammation. Differential diagnosis for hypoechoic pancreatic lesion includes chronic pancreatitis, neuroendocrine tumor, lymphoma, or metastatic tumor. EUS FNB has emerged as valuable diagnostic procedure for evaluating pancreatic lesion.
PP-01-089
Diagnostic yields and clinical impact of endoscopic ultrasound-guided tissue acquisition for biliary tract lesions
Daiki Agarie, Susumu Hijioka and Takuji Okusaka
National Cancer Center Japan, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed at clarifying the efficacy and safety of endoscopic ultrasound-guided tissue acquisition (EUS-TA) for diagnosing biliary tract lesions.
Materials and Methods: Puncture targets included patients with biliary tract lesions. Cases in which lymph nodes or liver metastases were punctured were excluded. Diagnostic yields of EUS-TA and adverse event rates were evaluated for each lesion site. Information on cases in which Endoscopic retrograde cholangiography (ERC) guided biopsy/cytology was performed was also collected.
Results: EUS-TA, attempted in 56 patients with biliary tract lesions, 45 were malignancy and 11 were benign respectively, had a sensitivity, specificity, and diagnostic accuracy of 95.6%, 100%, and 96.4% (54/56), respectively. The diagnostic accuracy for hilar bile duct lesions (90.5%; 20/22) was lower than that for the other sites (100%; 34/34; p=0.15). Procedure-related adverse events were observed in one case (1.8%) of mild bile leakage peritonitis with punctured hilar bile duct lesion.
Moreover, 217 cases of ERC guided biopsy/cytology were performed on suspected malignant biliary tract lesions in the study period, with diagnostic accuracy of 82.0% (178/217); the addition of endoscopic ultrasound-guided tissue acquisition for suspected false-negative cases resulted in an elevated diagnostic accuracy (93.1%; 202/217; p<0.001).
Conclusion: EUS-TA is an accurate and safe test for the diagnosis of biliary lesions. And it is also effective for the cases that are difficult to diagnose by ERC, thus the addition of EUS-TA should be considered when false negative ERC results are suspected.
PP-01-090
Efficacy EUS-BD in the treatment of malignant distal biliary and gastric/duodenal outlet obstruction
Catarina Budyono and Rabbinu Rangga Pribadi
Fellow of Gastroenterohepatology Department of Internal Medicine Faculty of Medicine Universitas Indonesia / Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia;Division of Gastroenterology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Endoscopic ultrasonography-guided biliary drainage (EUS-BD) is one of the alternative methods in treating malignant biliary obstruction (MBO) when endoscopic retrograde cholangiopancreatography (ERCP) cannot be performed due to inaccessibility. This method is classified into three techniques, i.e. EUS-guided choledocoduodenostomy (EUS-CDS), EUS-guided hepatogastrostomy (EUS-HGS) and EUS-guided antegrade stenting (EUS-AGS).
Case Report: A 44-year-old female was consulted from digestive surgeon with chief complaints of jaundice since a month ago and fever since 1 week ago. Physical examination showed icteric sclera and skin. Laboratory examination showed increased leukocytes, decreased hemoglobin and hematocrit. In addition, total bilirubin 14.92 mg/dL, direct bilirubin 10.5 mg/dL, and indirect bilirubin 4.42 mg/dL. MRI-MRCP examination showed solid mass at pancreatic head and body, with CBD stricture and upstream dilatation of CHD and bilateral intrahepatic bile duct. When ERCP was performed, there was stenosis and duodenal bulb ulcer, so the coverage could not reach D-2. Five days post EUS-AGS and EUS-HGS, there was a decrease in total, direct, and indirect bilirubin levels to 4.8 mg/dL, 3.41 mg/dL, and 1.39 mg/dL respectively. In addition, acute cholangitis resolved and the patient underwent biliodigestive bypass.
Discussion: EUS-BD is expected to be the main alternative in managing biliary drainage. When compared to percutaneus transhepatic biliary drainage (PTBD), which is also an alternative to ERCP, EUS-BD has a higher success rate and lower complication rate. EUS-BD also provides better comfort and nutrient absorption, and avoids electrolyte loss. The technical and clinical success of EUS-BD is higher than ERCP in pancreatic patients with indwelling gastro-duodenal stents.
PP-01-091
Paradigm shift: Case report of synergistic use of eus-guided gastrojejunostomy(EUS-GJ) and Hepaticogastrostomy(EUS-HGS) for malignant obstruction
Wah Loong Chan, Farahani Khamis, Khairiah Ahmad and Stanley Khoo
University Of Malaya, Kuala Lumpur, Malaysia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To report the successful use of endoscopic ultrasound-guided gastrojejunostomy (EUS-GJ) and hepaticogastrostomy (EUS-HGS) in managing gastric outlet and biliary obstructions in a patient with metastatic pancreatic adenocarcinoma.
Case Description: A 47-year-old female with metastatic neck of pancreas adenocarcinoma, previously treated with FOLFIRINOX, presented with post-prandial vomiting, abdominal pain, and lethargy. Clinical examination revealed jaundice and epigastric tenderness. Past interventions included duodenal stenting and EUS-guided biopsy confirming adenocarcinoma.
Results: Laboratory tests showed worsening liver function with hyperbilirubinemia (126 μmol/L), Alanine Transaminase (ALT) 449 U/L, Aspartate Aminotransferase (AST) 214 U/L, Gamma-glutamyl Transferase (GGT) 721 U/L, and Alkaline Phosphatase (ALP) 363 U/L. CA 19-9 had increased to 5882 U/mL. Gastroscopy revealed a displaced and disintegrated duodenal stent with tumoral invasion. Positron emission tomography–computed tomography (PET-CT) demonstrated disease progression with worsening metastases and biliary obstruction. EUS-GJ was performed using a 20mm x 10mm electrocauterylumen-apposing metal stent (LAMS). EUS-HGS was completed using a 10cm partially covered hybrid self-expandable metal stent (SEMS). Post-procedure, the patient’s liver function improved and she resumed a soft diet.
Discussion and Conclusion: This case demonstrates the success of combined EUS-GJ and EUS-HGS in managing complex malignant obstructions. Some studies demonstrated that EUS-GJ to be superior to enteral stenting, with lower reintervention rates and higher clinical success rates. EUS-HGS has emerged as a viable alternative to percutaneous drainage. The positive outcome in this case highlight the importance of learning these advanced techniques from local and international experts to elevate the standard of care in managing patients with advanced malignancies.
PP-01-092
Comparing safety, clinical success, and re-intervention in EUS-gastroenterostomy vs. Enteral metal stents in malignant GOO
Arun Arora Pagadapelli and Amit Daphale and Rohan Yewale and Amol Bapaye
Deenanath Mangeshkar Hospital, Pune, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
This study aimed to compare the safety, clinical outcomes, and rates of re-intervention between two palliative treatments for malignant gastric outlet obstruction(GOO): EUS-guided gastroenterostomy(EUS-GE) and enteral self-expanding metal stents(SEMS). Over 3.5 years(December 2019–August 2023), a retrospective analysis was conducted on 50 patients(20 EUS-GE, 30 enteral SEMS). Both groups, had mean ages around 62 years, predominantly male. Technical success rates were 90% for EUS-GE and 100% for enteral SEMS, with comparable clinical success rates. Mean hospital stays were 4.8 days ± 2.9 days for EUS-GE and 3.5 days ± 3.0 days for enteral SEMS (p=0.133). Most common cause for GOO in both groups was Carcinoma head of pancreas. Location of GOO was Proximal duodenum in 75% of patients in EUS-GE group and 66.7% in Enteral SEMS group(p=0.529), antrum in 25% of patients in EUS-GE group and 33.7% in Enteral SEMS group. Re-intervention was not required in the EUS-GE group, while two patients in the enteral SEMS group needed it(p=0.16). Median survival was similar in both groups, approximately 5.4 ± 4.6 months for EUS-GE and -5.5 ± 4.2 months for enteral SEMS(p=0.976).
Study concluded that both EUS-GE and enteral SEMS showed comparable efficacy in terms of technical success, clinical outcomes, safety profiles, and re-intervention rates. However, the limitation of a median survival of less than 6 months for both groups might have influenced outcomes. For patients with expected survival less than 6 months, enteral SEMS appeared non-inferior. Future studies focusing on long-term survival are necessary to determine if one method is superior.
PP-01-093
EUS guided Hepatic Solid mass (HSM) biopsy: The New Paradigm?
Pritam Das and Samir Mohindra and Dhruv Thakur and Gourav Borah and Naganath K Wodeyar and S Rakesh Kumar
KGMU, Lucknow, India, Lucknow, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: The advent of EUS guided procedures expanded the horizon of diagnostic and therapeutic procedures with real-time imaging and greater sensitivity of detection of smaller lesions (< 1 cm). EUS guided Hepatic Solid mass (HSM) biopsy had advantages over the traditional method (Percutaneous Ultrasound Guided) with ability to improved detection rate of smaller lesions (< 1 cm), diagnosis of nature of the lesion as well as in accordance to surrounding structures, performing therapeutic procedures (e.g., biliary drainage).
Method: This was a hospital based, observational prospective pilot study conducted in a tertiary centre in a period of one year. We included all patients of age ≥ 18 years old referred for liver biopsy for HSM. The aim of the study was assessment of the diagnostic yield and safety of Endoscopic Ultrasound Guided liver biopsy (FNB) in HSM. The primary outcome Measures included diagnostic adequacy of the tissue sample.
Results: The study included a total of 34 patients, of which FNB was done in 32 patients. Diagnostic Adequacy was present in 29/32 (90.6%) patients, inability to diagnose 3/32 (9.4%). The total number of core samples taken were 3.46 ± 1.29. The mean size of longest intact core was 6.625 ± 4.399 mm. The total sample length was 12.531 ± 6.968 mm.
Conclusion: Although the use of 16G needle by percutaneous method increases the diagnostic accuracy of the pathology specimen, EUS guided method (19G, 22G) yielded good diagnostic yield with minimal complication rates inspite of relatively smaller longest intact core sample.
PP-01-094
Evaluation of cryoablation using a prototype cryoablation needle in swine liver
Jonghyun Lee1,2, Hyunjoon Son3, Tae In Kim1,2, Dong Uk Kim4, Daejin Kim3, Gun-Ho Kim3 and Sung Yong Han1,2
1Division of Gastroenterology, Department of Internal Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, South Korea;2Department of Internal Medicine, School of Medicine, Pusan National University, Busan, South Korea;3Department of Mechanical Engineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea;4Department of Internal Medicine, CHA Gumi Medical Center, CHA University, Gumi -si, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background/Aims: Pancreatic cancer presents a significant challenge due to its late-stage diagnosis and high mortality rate. Cryoablation, a method employed for treating several cancer types, has shown promise in combination with other therapies for improving the prognosis of pancreatic cancer; however, its application is hindered by limitations such as lengthy procedures and specialized equipment. This study focused on developing a cryoablation needle suitable for endoscopic ultrasonography that could facilitate easier application in pancreatic cancer treatment.
Methods: This study involved cryoablation experiments on swine liver tissue using cryo-needles to assess the extent of cell death at various cryoablation temperatures and durations.
Results: The cryoablation system utilized liquid carbon dioxide and achieved rapid cooling, reaching temperatures below -60°C within 30 s and sustaining cryoablation for 200 s. These conditions induced liver tissue necrosis. Significant cell changes were observed up to 15 mm from the cryoablation needle.
Conclusions: This experimental study demonstrated the effectiveness of cryoablation with a cryo-needle in the swine liver. Further trials on pancreatic tissue are anticipated to confirm its effectiveness, with ongoing research crucial to establishing its value as an adjunct therapy.
PP-01-095
EUS provocation test to identify the origin of unexplained upper abdominal pain
Hyun Don Joo
Hanyang University, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Identifying the source of abdominal discomfort can be challenging, necessitating direct stimulation of the affected organ. However, achieving accurate stimulation is sometimes difficult. This study proposes a new method using endoscopic ultrasound (EUS) to pinpoint the origin of unclear upper abdominal pain.
Materials and Methods: Patients with unexplained upper abdominal pain, despite undergoing extensive medical evaluations, were enrolled. They underwent an EUS provocation test (EPT), which involved endoscopic inspection and mechanical stimulation such as stretching, torsion, and compression. A positive EPT result was determined if the patient experienced pain similar to their usual discomfort.
Results: From January 2015 to December 2020, EPT identified the origin of pain in 15 out of 17 patients (88.2%). Gastric stimulation yielded positive results in nine patients, cholecystic stimulation in three, and pancreatic stimulation in three. Two patients experienced pain that was not localized and resembled their usual discomfort.
Conclusion: This study demonstrates that EPT, a novel application of EUS, has a high sensitivity for identifying the origin of ambiguous abdominal pain. Targeting the organs identified through EPT could enhance treatment effectiveness.
PP-01-096
Outcome of ultrasound endoscopic gallbladder drainage for acute cholecystitis at single institution
Fumio Kakizaki
Cancer and Infectious Disease Center Komagome Hospital, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: EUS-guided gallbladder drainage (EUS-GBD) has been reported in patients with poor surgical tolerance. However, indications, techniques, equipment, and efficacy of EUS-GBD procedure are inconsistent and are equivocal in Japan. Therefore, this study aimed to evaluate the results and safety of EUS-GBD cases.
Materials and Methods: This study retrospectively evaluated the clinical characteristics of 10 patients who underwent EUS-GBD at Komagome Hospital between July 2018 and May 2024.
Results: (Patient Background) Median age was 84.5 years, male/female: 4/6, ASA-PS 0/1/2/3: 3/3/2/2, mean hospital stay was 30.4 days, and background diseases were dementia/degraded ADL/terminated (including benign and malignant)/other (failure of other treatments, comorbidities): 3/1/3/3. (Procedure details)Approach site: duodenal bulb/gastric: 9/1, stent type: plastic stent /ENBD: 9/1, fistula dilation rate: 90% (9/10), fistula dilation type: electric current/electric current + mechanical/mechanical: 7/1/1, procedure time: insertion to puncture/puncture to stent placement: 10 min /14.5 min. The procedural success rate was 90%, and the reason for failure was stent migration. The clinical success rate was 89% (8/9), and the reason for failure was prolonged fever. The complication rate was 20% (2/10), including two complications (perforation and stent migration) observed in the early induction stage of the EUS-GBD procedure, but have been treated without complications since then.
Conclusion: The EUS-GBD procedure is a relatively safe and effective EUS technique for patients with acute cholecystitis in the late stages of dementia or cancer.
PP-01-097
Atypical endosonographic features of pancreatic neuroendocrine tumors
Valeriia Kamalova, Evgeniy Solonitsyn, Dmitriy Baranov and Evgeniy Lebedev
Almazov Nmrc, Saint Petersburg, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: It is generally accepted that pancreatic neuroendocrine tumors (PNETs) on endosonography always appear as solid hypoechoic tumors, often multiple, with clear smooth edges, and hypervascular on Dopler scan. As experience with such patients accumulated, we noted that the appearance of PNETs can be highly variable; this study is dedicated to the descriptions of these cases.
Materials and Methods: This is a retrospective single-center study conducted from 2020 to 2024. Patients with PNETs verified by EUS-FNA (Endoscopic Ultrasound-Guided Fine Needle Aspiration) were included in the study. The study included 55 patients.
Results: Among the 55 patients, the male-to-female ratio was 1:1.66, with an average age of 62 (range 18-81). Neuroendocrine tumors were located with equal frequency in the head, body, and tail of the pancreas; in 23.2% of cases, multiple lesions were noted. The average size of the tumors was 30 mm (range 5-80 mm). Among the 55 tumors, 7.2% appeared as cystic-solid lesions, and 5.4% as cystic. In some cases, the PNETs had an unclear irregular border (5.4%), and a small portion were avascular on Doppler imaging (3.6%). All patients underwent EUS-FNA for diagnostic verification, and in 19.8% of cases, the presumed diagnosis based on the EUS image was not confirmed.
Conclusion: When performing EUS, it is important to remember the characteristic appearance of tumors; in some cases, to establish the correct diagnosis, it is necessary to correlate clinical data, visual characteristics, and perform EUS-FNA.
PP-01-098
Endoscopic ultrasound liver biopsy (EUS-LB) vs trans jugular liver biopsy (TJ-LB), in a special population
Shivam Khare and Anil Arora
Sir Ganga Ram Hospital, Delhi, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Gold standard route for liver biopsy is percutaneous-liver-biopsy(PC-LB) in absence of coagulopathy, thrombocytopenia and ascites.Trans-jugular-liver-biopsy(TJ-LB) is consider main route,in presence of coagulopathy,thrombocytopenia and ascites.Now,there is enough data available which showed non-inferior efficacy and safety of EUS-guided-liver-biopsy(EUS-LB) in comparison to PC-LB.EUS-LB offers certain advantages over traditional methods such as,one can obtain good sample in presence of ascites by getting ascites free window and similarly,in early coagulopathy by choosing vessel free approach on doppler.TJ-LB is a cumbersome procedure,therefore, Aim of the study is to compare the EUS-LB and TJ-LB in terms of adequacy and safety,in a special population where PC-LB is contraindicated but EUS-LB can perform(ascites/mild coagulopathy{INR between 1.5-2}) instead of TJ-LB.
Methods and material: This is a retrospective study. Adequacy of tissue diagnosis and Adverse events were compared between the two groups.
Results: 50 patients were enrolled in TJ-LB group, 40 in EUS-LB. Both groups were propensity matched in terms of age,gender,ascites,INR and child status. Total specimen length(TSL) and number of complete portal tract(CPT) were more in EUS-LB group than in TJ-LB group(3.5±0.9cm vs 1.7±1cm with p-value 0.000 and 19±11 vs 10.6±5.7 with p-value 0.000, respectively).Fragmentation was higher in EUS-LB vs TJ-LB(4.5±2.3 vs 2.7±1.3, p<0.05),However, longest fragment length were significantly higher in EUS-LB vs TJ-LB(mean 1.9cm vs 0.7cm).Adequate biopsy rate was 100% in EUS-LB and 96% in TJ-LB.No major complications occurred in the both group.
Conclusion: This first unique study from Asia is comparing EUS-LB and TJ-LB in special population.TSL,CPT,Adequate biopsy rate,Conclusive diagnosis and longest fragment length were more in EUS-LB than TJ-LB group.
PP-01-099
EUS-guided cystgastrostomy in pancreatic pseudocyst using electrocautery-enhanced lumen apposing metal stent delivery system
Hannah Angelica Lacar, Bin Chin, Josef Carlo Lazaro and Angela Djajakusuma
The Medical City, Pasig, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Pancreatic pseudocyst is an encapsulated collection of homogeneous fluid with little or no necrotic tissue located near the pancreas in patients with a history of pancreatitis. It occurs when the damaged pancreatic ducts cause extravasation and collection of the pancreatic fluid.
Case Description: The patient is a 44-year-old-female, diabetic, who experienced a 2-week period of intermittent crampy epigastric pain radiating to the back which progressed to persistent severe boring pain. On contrast CT scan, she had acute pancreatitis with peripancreatic fluid collection and was discharged. In the interim, she had abdominal fullness and on follow-up CT scan showed an increase in size of the well-defined fluid collections anterior to the pancreas, previously ascribed to a pseudocyst or walled off necrosis. A dilated pancreatic duct with abrupt cut off at the region of the pancreatic head. On EUS, there was a large well-encapsulated fluid collection posterior to the stomach measuring 15cm x 11cm. EUS-guided cystgastrostomy using Electrocautery-enhanced Lumen Apposing Metal Stent Delivery System was done and was able to drain 2 liters of pancreatic fluid. After 4 weeks, abdominal MRI MRCP showed no pancreatic ductal obstruction and on repeat EUS, there was a significant decrease in the pancreatic fluid collection. The stent was then successfully removed.
PP-01-100
Recurrent hematemesis unveiling gastric tuberculosis: A case report emphasizing the diagnostic value of endoscopic ultrasound
Ronell Lee, Mary Anne Go and Michael Louie Lim
Chinese General Hospital And Medical Center, Manila, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
In 2021, the Philippines ranked fourth globally in tuberculosis cases, with 650 individuals affected per 100,000, designating it as a high TB burden country. Gastric tuberculosis, though rare, poses diagnostic challenges due to clinical manifestations resembling more prevalent gastric pathologies. Gastrointestinal bleeding, typically associated with peptic ulcers, esophageal varices, or gastric malignancies, can occasionally indicate infectious processes like tuberculosis, especially in high-prevalence regions.
This report details the diagnostic journey of a 35-year-old male with recurrent hematemesis. Initial investigations, including upper gastrointestinal (GI) endoscopy and CT scans, failed to yield a definitive diagnosis. Subsequent GI endoscopy revealed a 2.0cm mucosal protrusion with a clean-based ulcer, emphasizing the importance of advanced diagnostic techniques like endoscopic ultrasound (EUS). EUS identified a hypoechoic lesion at the muscularis mucosa, with multiple hypoechoic lymph nodes. The fine-needle biopsy during EUS confirmed Mycobacterium tuberculosis (MTB) infection.
PP-01-101
Lower esophageal sphincter of achalasia patients under endoscopic ultrosongraphy : genesis and therapeutic outcomes
Cong Liu, Lili Zhang, Wei Zhao, Bin Whang, Hong Jin, Shujin Li, Shiwei Ru and Xuechai Liu
Tianjin Medical University General Hospital, Tianjin, CN, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the thickness and histological properties of lower esophageal sphincter (LES) in achalasia (AC) patients as well as its relationship with the prognosis of per-oral endoscopic myotomy (POEM).
Materials and Methods: Fifteen AC patients preparing for POEM as preliminary therapy and sixteen patients with submucosal tumor of stomach who accepted EUS as control group I were enrolled. Thickness of LES was measured and compared between groups. Ten patients who underwent distal esophagectomy for gastric cancer as control group II were also included, and the similar muscle was taken for histological evaluation. According to thickness of LES, AC patients were subdivided into thicker and thinner subgroups. The pathological features were assessed by H&E staining and compared between groups. In addition, the prognosis of AC were also compared between groups.
Results: The LES, internal circular muscle and outer longitudinal muscle of AC patients were thicker than that of control group I, respectively. AC patients showed severer atrophy and fibrosis than control group II. Furthermore, AC patients were subdivided to thicker and thinner LES group (with 3mm as standard), severer fibrosis was found in thicker group. There was no significant difference in response rate, Eckardt score and reflux rate between the thinner and thicker LES groups, while the Demesster score 2 years after POEM was higher in thicker group.
Conclusion: There was thicker LES of AC patients, which was associated with atrophy and fibrosis. AC patients with thicker LES under EUS was predictive of a severer GER two years after POEM.
PP-01-102
Comparison of EUS PV versus intra-operative direct pressure measurements in pre-transplant cirrhotic- pilot study
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Direct portal pressure measurement under EUS-guidance (EUS-PP) measurement is a promising alternative to conventional indirect Hepatic Venous Pressure Gradient (HVPG). In patients undergoing liver transplantation for cirrhosis, high pre-operative portal vein (PV) pressure requires intra-operative portal vein inflow modulation (PIM). This pilot study aims to assess correlation between EUS-PP with direct intra-operative portal pressure measurement (IO-PP).
Methods: Cirrhosis patients scheduled for liver transplant within next 48 hours were included. EUS-PPM was performed using a 22G EUS-FNA needle to puncture PV. Both, compact manometer (CM) and arterial pressure transducer (PT) were used to measure the intra-vascular pressure, pre- and intra-operatively. All adverse events were recorded. IO-PP was done just soon after laparotomy using PT.
Results: 25 patients, 23/25 were males. 19, 5 & 1 patients were CHILD PUGH C, B and A respectively.
Procedural technical success was 100 %. EUS guided pre-transplant PP using novel CM & PT was 27.12 +/- 6.2 mmHg and 31.68 +/- 10.2 mmHg, respectively. Intraoperative measurements just after laparotomy using CM & PT were 22.76 +/- 6.09 mmHg & 22.4 +/- 5.10 mmHg, respectively. 24-hour post liver transplant portal pressure measurements using CM & AT were 9.44 +/- 1.70 mmHg & 8.8 +/- 1.77 mmHg, respectively. Only 3/25 patients had mild hematomas and did not require any additional surgical intervention.
Conclusion: EUS guided direct PV puncture & PP measurement using compact manometer is a safe and correlates well with PT, both preoperatively & intra-operatively. Multi-centric validation with higher numbers study are needed.
PP-01-103
Endoscopic Ultrasound(EUS)-Guided Biopsy; A Diagnostic Tool in Lymphadenopathy Surrounding Gastrointestinal Tract: A tertiary center experience
1Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;2University Surgical Unit, National Hospital, Sri Lanka, Colombo;3Department of Pathology, Faculty of Medicine, University of Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: EUS with transluminal biopsy has revolutionized the diagnosis of patients with enlarged lymph nodes (LN) of the mediastinum, para-aortic and hilar regions where traditional more invasive surgical approaches were the gold standard. This pioneering study in Sri Lanka highlights the effective use of EUS in obtaining histology of LNs in these sites with difficult access.
Materials and Methods: From March 2018 to March 2024, 27 patients with LN masses requiring EUS-guided biopsy were included in this study. A single endoscopist (ANR) performed the procedures.
Results: The mean age was 49.44 years (SD 15.52) with 16 (59.3%) male patients. LN locations were as follows: 11 (40.7%) para-aortic, 5 (18.5%) porta hepatis, 4 (14.8%) mediastinal, 4 (14.8%) celiac, and 3 (11.1%) para-oesophageal. The LN sizes ranged from 10 to 47 mm. Diagnoses were available in 21 (77.8%) cases, in 6 the diagnosis remained inconclusive. Among the positive diagnoses, 11 (52.4%) were malignant, 7 (33.3%) benign, and 3 (14.28%) infective. The diagnoses included 4 (18.5%) Adenocarcinoma, 3 (11.1%) Hodgkin lymphoma, 2 (7.4%) non-Hodgkin lymphoma, 2 (7.4%) poorly differentiated carcinoma, and 3 (11.1%) tuberculosis cases. No complications occurred during the procedure.
Conclusion: EUS-guided biopsy is a safe and effective diagnostic tool for LN masses accessible via the upper gastrointestinal tract, providing a non-invasive alternative to surgical methods.
PP-01-104
Endoscopic Ultrasound(EUS) guided biopsy as a diagnostic tool in upper-gastrointestinal(UGI) lesions, A tertiary center experience
1Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;2University Surgical Unit, National Hospital, Colombo, Sri Lanka;3Department of Pathology, Faculty of Medicine, University of Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Despite its widespread use internationally, EUS-guided transluminal biopsy of UGI lesions is rarely requested and therefore no data is available in Sri Lanka. Without histology, these patients may have had to undergo surgery or follow-up with prolonged anxious periods. This study outlines the experience of a tertiary care referral center.
Materials and Methods: From March 2018 to March 2024, EUS-guided biopsy was performed on 46 patients with UGI tract lesions by a single endoscopist (ANR).
Results: The mean age was 57.93 years (SD=16.375) with 30 (65.2%) males. Lesion sites were, stomach (n=17,37.0%), gastro esophageal junction (GOJ) (n=10, 21.7%), duodenum (n=10, 21.7%) and esophagus (n=9, 19.6%). The majority were solid (n=42, 91.35%), sizes ranging from 12 -34mm. No complications were seen.
A diagnosis was made in 91.3% (n=42) cases with only 2 (4.3%) inconclusive and 2 (4.3%) inadequate tissue. 16 (34.8%) were malignant. Following are location specific diagnoses. Esophageal: Gastrointestinal Stromal Tumors (GIST) (n=2), spindle cell tumour (n=2) and normal tissue (n=2). GOJ: Leiomyoma (n=4), squamous cell carcinoma (n=2), GIST/leiomyoma (n=2), normal tissue (n=1). Stomach: GIST (n=6), poorly cohesive carcinoma (n=1), GIST/leiomyoma (n=2), spindle cell tumour (n=2), gastric adenoma (n=2). Duodenum: Adenocarcinoma (n=3), GIST (n=2), Neuro Endocrine Tumor (n=2), Poorly cohesive carcinoma (n=2).
Conclusion: EUS guided biopsy is an effective and safe diagnostic tool for UGI lesions which can be utilized in Sri Lanka to provide histological diagnosis and determine appropriate treatment.
PP-01-105
EUS-guided coil in the management of gastric varices- A single center case series in Malaysia
Ram Prasad Sinnanaidu and Khoo Stanley
University Of Malaya, Kuala Lumpur, Malaysia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Aims: Gastric varices (GV) have high rates of rebleeding and mortality. Current endoscopic therapies are premised on endoscopic cyanoacrylate injection, a non-targeted therapy and carries a higher risk of rebleeding. Endoscopic Ultrasound (EUS) guided therapy in lately has gain much attraction in the management of GV. We present a case series of EUS guided coiling in combination with cyanoacrylate (CYA) injection for the treatment of GV.
Methods: Six patients with GV underwent EUS guided coiling and CYA injection from July 2021 to December 2023. Embolization coils (size 12mm to 16mm) were inserted under EUS guidance followed by CYA injection. Obliteration of GV was confirmed by doppler imaging and endoscopic visualization. All patients were given prophylactic antibiotics. The baseline characteristics, technical success, clinical success, rebleeding rates and adverse events were recorded.
Results: Among the 6 patients included, the most common etiology of GV was cirrhosis (83%), with MAFLD being the most common cause (80%). The main indication for therapy was primary prophylaxis (50%). 67% had GOV-2 with mean GV size of 18±6.0mm and the mean number coils used were 2±1.1. All patients achieved technical and clinical success. There were no intraprocedural adverse events, however 3 patients developed low grade fever post procedure requiring antibiotics and hospital admission. Only one patient had delayed rebleeding after 6 weeks.
Conclusions: Combination of EUS coil and CYA therapy is an effective management for gastric varices with acceptable safety profile. Transient fever seen in half of the patients, was an interesting observation which needs further exploration.
PP-01-106
Replacement technique for dislocated lumen-apposing metal stent in endoscopic necrosectomy by the forceps
氏 Hiroshi Takihara
Uji Tokushukai Hospital/ Gastroenterology, kyoto, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Lumen-Apposing Metal Stents(LAMS) are essential for endoscopic necrosectomy(EN) in severe pancreatitis. However, LAMS often drops out during EN. We present a simple method to replace a dislocated LAMS by a forseps.
Case Description: A 52-year-old man suffered from severe pancreatitis caused by gallstones. He was treated with endoscopic sphincterotomy(EST) and recovered well. But 1 week later,He developed septic shock and abdominal compertment syndrom with massive ascites. We urgently performed percutaneous drainage and placed LAMS(Hot-AxiosTMstent20mm) in posterior wall of gastric antrum.
3weeks later,we performed EN in the walled-off necrosis(WON). There was massive necrotic tissue and we reperted EN 3times.
During necrosectomy, LAMS interfered with snare operations. then we removed the LAMS through the esophageal overtube and after EN, we replaced the LAMS by a forseps.
8th days later necrosectomy, there were delayed bleeding of pseudoaneurysm. So we plosedured percutaneous vascular embolization. After that, He recovered well at all.
Discussion: It is a simple method to replace a dislocated LAMS by the forseps. This case had a large cavity, it was easy to replace the LAMS. If the cavity is not enough to replace it, we have to plosedure another method like “through the channel replacement”.
PP-01-107
The diagnostic performance of endoscopic ultrasound guided fine-needle biopsy of solid pancreatic lesion
Nicholas Tee, Weicong Lin, Weiquan Li, Andrew Boon Eu Kwek and Tiing Leong Ang
Changi General Hospital, Singapore, Singapore
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Endoscopic ultrasound (EUS) - guided tissue acquisition is important to establish a histological diagnosis of solid pancreatic lesions. The objective of this study is to evaluate the diagnostic performance of EUS-guided fine needle biopsy (FNB) of solid pancreatic lesions performed in our endoscopy center compared to the recommended societal standards.
Materials and Methods: Data from patients who underwent EUS-guided FNB for solid pancreatic lesions, were retrospectively retrieved from January 2020 to December 2023. The overall sensitivity, specificity, accuracy of diagnosis and sample adequacy were analyzed. The diagnostic performance between 20G, 22G and 25G FNB needles were also analyzed. The FNB needles primarily used in our center were EchoTip ProCore needles, Cook Medical. All EUS procedures were performed by credentialed endosonographers.
Results: A total of 167 patients were analyzed. The overall tissue acquisition rate was 92.8%. The overall sensitivity, specificity and diagnostic accuracy were 90.8%, 100% and 92.2% respectively. With regards to tissue sample adequacy, the tissue acquisition rates for 20G (n=114), 22G (n=37) and 25G (n=16) FNB needles were 92.9%, 89% and 100% respectively. The 20G needle had a 90.7% sensitivity and a diagnostic accuracy of 91.2%, compared to 22G (sensitivity, 90%; accuracy, 91.9%) and 25G (sensitivity, 93.8%; accuracy 93.8%) needles.
Conclusion: The diagnostic performance of EUS-guided FNB of solid pancreatic lesions performed at our endoscopic center has met the standards for both tissue acquisition and sensitivity rate ≥ 85%. Further studies are required to evaluate any significant difference in diagnostic accuracy between the different needle sizes.
PP-01-108
Diagnostic yield and Safety for Pancreatic Cystic Lesions: Comparison of EUS-FNA and EUS-FNB
Mingmei Ye1, Xiaoyu Yu1, Yawen Ni2, Qianqi Liu1, Pan Gong1, Yuanyuan Huang1, Xiaoyan Wang1 and Li Tian1
1The Third Xiangya Hospital of Central South University, Changsha, China;2Nanchang University Queen Mary School, Nanchang, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The incidence and detection rate of pancreatic cystic lesions (PCLs) is increasing in recent years. Endoscopic ultrasound (EUS) plays an irreplaceable role in the diagnosis and differential diagnosis of PCLs, but the evidence on the comparative diagnostic performance of EUS-guided fine-needle aspiration (EUS-FNA) and fine-needle biopsy (FNB) is limited. So, we aimed to compare the diagnostic yield, specimen adequacy, and safety of EUS-FNA and FNB in PCLs to help guide current clinical practice.
Materials and Methods: Between January 2014 and August 2021, patients with PCLs who underwent EUS-FNA or FNB were retrospectively enrolled. The primary outcome was the diagnostic yield of EUS-FNA and FNB. The secondary outcomes were specimen adequacy and adverse events.
Results: A total of 90 patients were included (52 in FNA and 38 in FNB). The diagnostic yield was similar in the FNA group compared with FNB (94.2% vs 94.7%, P>0.05). The specimen adequacy was 71.2% and 81.6% in FNA and FNB, respectively (P>0.05). In terms of adverse events, there was no statistical difference(P>0.05).
Conclusion: EUS-FNA and EUS-FNB showed equally high diagnostic rate and specimen adequacy in PCLs with an excellent safety profile. Both FNA and FNB may be good and safe diagnostic tools for PCLs.
PP-01-109
Cold water infusion can improve the image quality of the miniprobe endoscopic ultrasonography under sedation
Xiaoyu Yu1, Mingmei Ye1, Jiangtao Liao2, Yang Hu3, Min Guo4, Bin Zeng5, Juan Li2, Liu Peng3, Wenfang Hu4, Han Liu5, Pan Gong1, Yu Long1, Xiaoyan Wang1 and Li Tian1
1The Third Xiangya Hospital of Central South University, Changsha, China;2the Hunan Provincial People’s Hospital, Changsha, China;3the Affiliated Nanhua Hospital of University of South China, Hengyang, China;4the First People’s Hospital of Changde City, Changde, China;5the First Affiliated Hospital of University of South China, Hengyang, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Miniprobe endoscopic ultrasonography (mEUS) plays an increasingly significant role in gastrointestinal (GI) tumors. Previous studies showed the water temperature could affect patients’ GI peristalsis and safety, however, the optimal water temperature for mEUS remains uncertain. We aimed to investigate the effects of different water temperatures on mEUS under sedation.
Materials and Methods: We conducted a prospective, multicentre, double-blind, randomized study. Patients were randomly assigned to the cold (6–10 °C, CG), warm (20–24 °C, WG), and hot (35–39 °C, HG) water groups. The primary outcome was the image quality. Secondary outcomes included diagnostic accuracy, peristaltic grade, haemodynamics (T0–T5), comfort and satisfaction scores, and adverse events.
Results: 240 patients were randomly assigned, with 80 patients in each group, between June 2022 and March 2023. The percentage of images with a quality score of 5 in the CG, WG, and HG was 88.8%, 75.0%, and 65.0%, respectively (P = 0.002); the diagnostic accuracy of the CG was higher than that of the WG and HG, although not statistically different (97.0% vs 81.5% vs 81.1%, P = 0.083). The CG had significantly lower peristaltic scores after water infusion than the WG and HG (P<0.001). No serious adverse events were observed. The comfort and satisfaction scores were similar.
Conclusion: The CG was more advantageous than the WG and HG in reducing GI peristalsis, further improving image quality with excellent safety. Cold water (6–10 °C) may be the optimal water temperature for mEUS under sedation, with the potential to improve the diagnostic accuracy.
PP-01-110
Mastering Excellence: Learners' Insights into Deliberate Practice and Simulation-Based Learning
Tonya Kaltenbach1,2,3, Roy Soetikno1,2,3, Hasan Maulahela1,4, Carlos Paolo Francisco1,5, Mark Anthony De Lusong1,6, Eric Yasay1,6, Enrik John Aguila1,5, Yung Ka Chin1,7, Wen-Feng Hsu1,8, Ruter Maralit6, Jonard Co5, Yasuhisa Abe1 and Patricia Anne Cabral-Prodigalidad1,5,6
1Academy of Endoscopy, Woodside, United States;2Department of Gastroenterology and Hepatology, San Francisco VA Medical Center, San Francisco, United States;3Department of Medicine, University of California, San Francisco, United States;4Faculty of Medicine University of Indonesia - Cipto Mangunkusumo General Central National Hospital, Jakarta, Indonesia;5Institute of Digestive and Liver Diseases, St. Luke’s Medical Center - Global City, Taguig City, Philippines;6Department of Gastroenterology, Department of Medicine, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines;7Mount Elizabeth Hospital, Singapore;8Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction and Objectives: Deliberate Practice (DP) is the most effective method to develop expertise in all disciplines. This approach allows learners to practice specific skills repeatedly until they reach a predetermined level of mastery. Simulation-Based Mastery Learning (SBML) is a stringent form of competency-based education that leverages the principles of DP. By using case-based simulations, SBML creates a structured and controlled learning environment, ensuring that learners achieve a higher level of proficiency.
Traditional training methods often involve passive learning, where learners acquire theoretical knowledge without ample opportunity to practice the skills. In contrast, SBML engages learners in simulated scenarios, enabling them to gain hands-on experience in a risk-free setting. We have used SBML to provide a significantly more effective endoscopy training. During the training, learners focus primarily on mastering the content of the course. However, they are not explicitly taught the underlying principles of DP that make SBML so effective. Herein, we aim to share the learners' understanding of the details of the SBML method.
Methods and Results: We asked gastroenterology trainees (n=32) to provide reflections on their learning experience during a colonoscopy course. These reflections were recorded and analyzed using qualitative analysis to document their understanding of the SBML method used to learn the principles of colonoscopy. From this data, we created a video to showcase their insights.
Conclusion: Trainees of this SBML course exhibit a great understanding and appreciation of its principles. They expressed how they applied these principles effectively, allowing them to maximize their learning opportunities.
PP-01-111
Does artificial intelligence improves the quality of screening colonoscopy?
Sabina Guseinova1, Sergey Kashin1,2 and Dmitry Zavialov1,2
1Yaroslavl Regional Cancer Hospital, Yaroslavl, Russian Federation;2Yaroslavl State Medical University, Yaroslavl, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: According to WHO colorectal cancer is the third most common diagnosis in the world in 2022. The detection rates of polyps and adenomas are inversely proportional to the risk of interval colorectal cancer. They also assess the competence of an endoscopist. Deep neural network algorithms is a promising way to improve colonoscopy quality.
Materials and Methods: In prospective single-center study it was assessed the impact of AI-program for polyp detection during the screening colonoscopy (SC), which was performed by an experienced doctor from May to December 2023. Patients who had not undergone colonoscopy before were randomly assigned into 2 groups. All detected lesions were sent for histological examination. Then it was evaluated the colonoscopy quality indicators.
Results: The control group included 54 patients (mean age 61.7), the group of patients who had a SC with AI-program — 56 (mean age 60.9). The cecal intubation rate (CIR) was 100% both. All patients had bowel preparation more than 7 points on the BBPS; Respectively in control and study groups PDR was 0.52 and 0.56; ADR — 0.26 and 0.29; APC — 0.39 and 0.41; APP — 1.5 and 1.6; CWT — 8.39 and 11.48.
Conclusion: The use of the AI-software insignificantly improved colonoscopy quality indicators by qualified doctor, but significantly increased the colonoscopic withdrawal time. A comparative study will be conducted with the participation of endoscopists with less than 3 years of experience. This will reveal whether the AI-program can enhance the quality of colonoscopy performed by inexperienced endoscopists.
PP-01-112
Endoscopic interventions for malignant afferent loop syndrome: Which is the best treatment?
Tomohiro Kaketani
Tokyo Medical University Hospital, Shinjuku, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Afferent loop syndrome (ALS) is a life-threatened adverse event after gastrointestinal surgery requiring appropriate early decompression treatment. Several endoscopic interventions have been attempted for treatments such as endoscopic enteral metal stent placement (EMSP), endoscopic ultrasound (EUS)-guided entero- enterostomy (EUS-EE), and EUS-guided hepaticogastrostomy (EUS- HGS). However, there are limited data on outcomes, including duration of stent patency. Therefore, we evaluated the usefulness of each endoscopic intervention for malignant ALS.
Materials and Methods: We retrospectively collected data of 3 ALS patients each whom underwent EMSP, EUS-EE, and EUS-HGS. Information on technical success, clinical efficacy, adverse events, stent dysfunction, and overall survival was gathered and analyzed.
Results: All of the stent placement has done successfully, without any fatal event. Abdominal pain and cholangitis were the most frequent symptoms. Throughout the study, 2 patients treated by EUS-HGS suffered from stent dysfunction, 8 patients died of primary disease during a median follow-up of 157 days.
Conclusions: Choosing any of the endoscopic interventions for malignant ALS can be expected to produce similar outcomes, including duration of stent patency. The choice of endoscopic intervention should be made based on the characteristics of each treatment.
PP-01-113
Management of Chronic Hemorrhagic Radiation Proctitis: From Six Cases Treated with Endoscopic Argon Plasma Coagulation
Ida Mujahidah Kamaruddin1,2, Muhammad Luthfi Parewangi1,2, Fardah Akil1,2, Nu'man AS Daud1,2, Rini Rachmawarni Bachtiar1,2, Susanto Hendra Kusuma1,2, Amelia Rifai1,2 and Resha Dermawansyah Rusman1,2
1Division of Gastroentero-hepatology, Department of Internal medicine, Faculty of medicine, Hasanuddin University, makassar, Indonesia;2Centre of Gastroenterology-Hepatology HAM Akil, DR. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: A common treatment for gynecological cancers is radiation therapy, it can be helpful in treating cancers, but also cause collateral GI tract damage. While acute toxicity can manifest as proctitis or enteritis, the more concerning sign is the development of a chronic hemorrhagic radiation proctitis (CHRP). The hallmarks of CHRP included mucus discharge, tenesmus, hematochezia, and occasionally fecal incontinence. Endoscopic argon plasma coagulation (APC) therapy stops or significantly reduces rectal bleeding in 79% to 100% of patients whose rectal bleeding is caused by CHRP.
Case Illustration: Six cases that were initially evaluated at Dr Wahidin Sudirohusodo Hospital, Makassar, Indonesia. Each patient had pelvic radiation treatment for their malignancy, and they were all referred for ongoing transfusion-dependent hemorrhage due to radiation proctitis. The rectum has telangiectasis caused by radiation. After one to three APC Erbe Vio 200 S therapy sessions with a gas flow of 2.0 l/min and a temperature range of 40–60 W, all patients saw a significant reduction in their symptoms. After treatments, neither patient needed blood transfusions, and their hemoglobin levels improved. At the 3- and 6-month follow- up, they were both doing well.
Discussion: Radiation therapy, while effective against cancers like gynecological cancers, can lead to collateral damage in the GI tract, such as CHRP. This case illustration demonstrated that APC therapy successfully reduced rectal bleeding in patients with radiation-induced proctitis and eliminating the need for transfusions, with sustained benefits observed at follow-up appointments. We recommend colonoscopy screening for patient who underwent pelvic radiation therapy.
PP-01-114
LOCALLY ADMINISTERED PHOTODYNAMIC THERAPY FOR CANCER USING NANO=ADHESIVE PHOTOSENSITIZER
Yoshiki Komatsu1 and Toru Yoshitomi2 and Hirofumi Matsui
1University of Tsukuba Hospital, Tsukuba, Japan;2National Institute for Materials Science, Tsukuba, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Photodynamic therapy (PDT) is a great potential anti-tumor therapy owing to its non-invasiveness and high spatiotemporal selectivity. However, systemically administered photosensitizers diffuse in the skin and the eyes for a long duration, which cause phototoxicity to bright light and sunlight. Therefore, following PDT, patients must avoid exposure of to light and sunlight to avoid this phototoxicity. In this study, we have developed a locally administered PDT using nano-adhesive porphyrin with polycations consisting of quaternary ammonium salt groups (aHP) as a photosensitizer. The aHP, approximately 3.0 nm in diameter, adhered the negatively charged cell membrane via electrostatic interaction. The aHP localized to the endosome via cell adhesion and induced apoptosis upon 635 nm light irradiation. On being administered subcutaneously on the tumor, 30% of the injected aHP remained in the administered sites. However, low-molecular-weight hematoporphyrin dihydrochloride (HP) disappeared due to rapid diffusion. PDT with locally administered aHP showed a higher anti-tumor effect after light irradiation at 635 nm for three days compared to low-molecular-weight HP. Intraperitoneal administration of HP caused severe phototoxicity upon irradiation with ultraviolet A at 10 J cm-2, whereas aHP did not cause phototoxicity because its diffusion into the skin could be suppressed, probably due to the high-molecular weight of aHP. Therefore, locally administered PDT with aHP is a potential PDT having high therapeutic efficacy without phototoxicity.
PP-01-115
A Meta-analysis on Endoscopic Gastric Balloon Placement versus Endoscopic Sleeve Gastroplasty for Management of Obesity
Natasha Bernadine Mapa and Marc Julius Navarro
Makati Medical Center, Makati City, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Managing obesity that does not respond to lifestyle change or pharmacotherapy poses significant challenges, especially in determining the best intervention. The situation emphasizes the challenge of maintaining weight loss over time and the importance of using comprehensive strategies to effectively address obesity. In the recent years, bariatric surgery, such as gastric bypass and sleeve gastroplasty, offers significant weight loss and metabolic advantages, especially for those with severe obesity or obesity-related health conditions.
Methodology: A meta-analysis was performed using 4 studies which directly compared the effectiveness of endoscopic gastric balloon (EGB) placement versus endoscopic sleeve Gastroplasty (ESG) for management of obesity. Electronic databases such as The Cochrane Library, Pubmed, Google Scholar, and Science Direct were searched for relevant studies on May 01 2024 using the search strategy: (gastric balloon placement) AND (sleeve Gastroplasty) AND (obesity). Random effects meta-analysis was performed to compute for mean difference and risk ratio to pool individual study data.
Results: The pooled mean difference showed that ESG is associated with higher %TBWL than EGB at 1 month (MD=-2.3, 95%CI=-4.1 to -0.6, p=0.009) and at 12 months (MD=-7.6, 95%CI=-10.8 to -4.5, p<0.00001). No significant difference was observed in the 6 months period (MD=-2.3, 95%CI=-5.3 to 0.7) and in terms of adverse events (RR=1.6, 95%CI= 0.2 to 12.8).
Conclusion: We observed a higher benefit of ESG for weight loss compared to EGB among obese individuals. Both interventions are equally safe and not associated with serious adverse events.
PP-01-116
Acute pancreatitis from compressive effect of intragastric balloon auto-inflation due to Candida infection
Yan Ling Ong1, R Rajesh1,2, Carlos Paolo Francisco3 and Ravishankar Asokkumar1,2
1Gastroenterology and Hepatology, Singapore General Hospital, Singapore;2DUKE- NUS Graduate Medical School, Singapore;3Institute of Digestive and Liver Diseases, St Luke’s Medical Centre-Global City, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Obesity is a metabolic disorder with considerable health implications affecting populations worldwide. Intragastric balloon (IGB) placement has gained popularity in the recent years as a less invasive and reversible management option for Obesity in comparison to bariatric surgery. IGBs are safe but can have rare adverse outcomes.
Case Description: We present a rare case of IGB auto-inflation due to Candida infection that resulted in local compressive effects on the pancreas and resulted in pancreatitis. Urgent endoscopic removal of the balloon resulted in complete resolution of the pancreatitis and symptoms.
Discussion: Spontaneous balloon hyperinflation resulting in local compression on the pancreas has been described to be related to bacterial and fungal overgrowth. The management of complications related to IGB placement including IGB-induced acute pancreatitis typically involves early recognition and balloon removal to prevent further progression.
PP-01-117
Post procedural pain management following endoscopic sleeve gastroplasty
Yan Ling Ong1, Charlene Tan Hui Ru1, R Rajesh1,2, Carlos Paolo Francisco3 and Ravishankar Asokkumar1,2
1Gastroenterology and Hepatology, Singapore General Hospital, Singapore;2DUKE- NUS Graduate Medical School, Singapore;3Institute of Digestive and Liver Diseases, St Luke’s Medical Centre-Global City, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: There is growing concern that patients undergoing bariatric surgery are exposed to excessive opioids post-procedure with studies showing 6-10% of opioid naïve patients developing chronic dependence post-operatively. Endoscopic sleeve gastroplasty (ESG) is a minimally invasive alternative to bariatric surgery that is gaining popularity but currently, there is no established guideline on post-procedural pain management. The data on opioid use and dependance post-ESG is scant.
Methods: This was a retrospective study of patients who underwent ESG or revision-ESG in a tertiary medical center in Singapore from January 2020 to July 2024. The primary outcome was the number of patients requiring post-operative opioid therapy. Secondary outcomes included frequency and dosage of post-operative pain medications and post-operative pain scores.
Results: Of the 194 patients who underwent ESG or R-ESG, 62 patients (31.9%) developed post-procedural abdominal pain with a mean pain score of 3.4. These patients required low dose intravenous pethidine and paracetamol limited to one day while inpatient. They did not require post-operative patient-controlled analgesia (PCA) or further opioids. On discharge, they were given only one week of per requirement panadeine (paracetamol + codeine) and none required chronic analgesia thereafter. The mean amount of opioids prescribed expressed as morphine milligram equivalents (MME) is 10 MME on day 1 and 3.6 MME for 1 week, considerably less than post-bariatric surgery. None of the patients developed chronic opioid dependence.
Conclusion: ESG is a safe procedure with minimal post-procedure pain requiring limited use of analgesia including opioids, thereby mitigating the risk of chronic opioid dependence.
PP-01-118
Collagenous Ileitis: Rare Condition with Diagnostic Challenges
Fei Yang Pan1,2 and Ahmed Alrubaie3
1Macquarie University Hospital, Sydney, Australia;2Macquarie University, Sydney, Australia;3Bankstown-Lidcombe Hospital, Sydney, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Collagenous ileitis (CI) is a rare condition characterized by the thickening of the subepithelial collagen band in the ileum, presenting with symptoms such as diarrhea, altered bowel habits, and abdominal pain. CI is often under-recognized and challenging to diagnose due to its rarity and the need for advanced diagnostic procedures.
Case Description: A 77-year-old female presented with recurrent melena and iron deficiency anaemia. Anti-gliadin and anti-tissue transglutaminase IgA, IgG and stool MCS tests were negative. Faecal calprotectin was elevated at 213 μg/g, with negative ANCA and ASCA. Video capsule endoscopy and antegrade double-balloon endoscopy revealed abnormal nodular mucosa in the distal jejunum and proximal ileum. Biopsies of the proximal ileum showed mildly oedematous ileal mucosa with broadening of the villi and a patchy increase in the subepithelial collagen plate between 10 and 20 μm, establishing a diagnosis of CI. Treatment with 9 mg of budesonide daily led to symptom improvement. Follow-up colonoscopy is planned to reassess the condition and determine the duration of steroid therapy.
Discussion: CI remains a rare and under-recognized condition, often requiring double-balloon endoscopy for diagnosis. This case highlights the difficulty in diagnosing CI, particularly when the terminal ileum is not involved. Given that intermittent diarrhea is a common symptom, collagenous disorders of the small bowel should be considered in cases of refractory diarrhea. The patient’s improvement with steroids suggests a better prognosis compared to other collagenous disorders like collagenous sprue. Further research is essential to understand CI's pathogenesis and establish diagnostic and treatment guidelines.
PP-01-119
Fluoroscopy-Guided Endoscopic Balloon Dilatation for Corrosive Esophageal Stricture with Distal-to-Proximal Technique: A Prospective Case-Series Study
Sri Rahayu Paputungan1,2, Muhammad Luthfi Parewangi1,2, Fardah Akil1,2, Nu'man AS Daud1,2, Rini Rachmawarni Bachtiar1,2, Susanto Hendra Kusuma1,2 and Amelia Rifai1,2
1Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;2Centre of Gastroenterology-Hepatology HAM Akil, DR Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Abstract
Background: The corrosive esophageal stricture is the most common sequela of caustic ingestion, significantly impairing the physical and socioeconomic abilities of patients and posing a major therapeutic challenge. Endoscopic dilatation is the first-line management option. Corrosive strictures can affect every esophageal segments, often being multiple, long, irregular, and more resistant to dilation compared to other causes of benign stricture. Fluoroscopic-guided esophageal balloon dilation is safe and easy to perform, provided the operators are aware of potential complications and the procedure is properly tailored to the patient's underlying condition. Among the therapeutic options for enhancing the effects of endoscopic dilation, one novel technique is the intralesional injection of corticosteroids.
Case report: This case series study involves three patients with esophageal strictures due to corrosive injury who underwent fluoroscopic endoscopic balloon dilation at Wahidin Sudirohusodo Hospital. The procedure involved four to eight weekly dilations using a fluoroscopy-guided distal-to-proximal technique with a 15-20 mm balloon dilator, followed by a 40 mg intralesional injection of triamcinolone, totalling 26 procedures. The clinical targets were the relief in dysphagia and increasing body weight. All esophageal strictures were successfully dilated with no significant adverse events.
Discussion: Fluoroscopic endoscopic balloon dilatation using a distal-to-proximal technique was performed on three patients with long esophageal strictures due to corrosive injury. This technique reduced the procedure time, and can be performed in long, multiple strictures. Steroid injection may be helpful in reduced of required dilatation session.
PP-01-120
Comparative study protocol on the efficacy of remimazolam and midazolam in endoscopic sedation in Japan
1Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan;2Department of Gastroenterology, Saitama Saiseikai Kawaguchi General Hospital, Saitama, Japan;3Department of Gastroenterology, Internal Medicine, Kitasato University School of Medicine, Sagamihara, Japan;4Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan;5Clinical Research Center, Saga University Hospital, Saga, Japan;6Department of Endoscopic Diagnostics and Therapeutics, Saga University, Saga, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The need for sedation in gastrointestinal endoscopy is increasing and has become a highly important medical procedure in daily practice. Remimazolam is a newly developed, ultra-short-acting benzodiazepine and has a shorter pharmacokinetic half-life than midazolam. It is expected to shorten the time to awaken after an endoscopy and the time to leave the recovery room, making it useful for sedation in the endoscopy field. This prospective study (RECOVER study) was designed based on the hypothesis that remimazolam would be useful as a sedative agent in gastrointestinal endoscopy in Japanese patients.
Materials and Methods: This multicenter, randomized, controlled trial in Japan randomized patients aged 18-80 years undergoing upper gastrointestinal endoscopy or colonoscopy with sedation to either the remimazolam or midazolam group. The primary outcome will be the percentage of study patients who are ambulatory 5 minutes after the endoscopy. The secondary outcomes will be the success rate of sedation with a Modified Observer's Assessment of Alertness/Sedation (MOAA/S) score ≤ 4 before endoscopy and the dose of remimazolam and midazolam required to achieve sedation (MOAA/S score ≤ 4), etc. The sample size was calculated to be 80 cases (40 upper gastrointestinal endoscopies and 40 colonoscopies) with reference to the pilot study.
Results: The study is ongoing. (jRCT pending)
Conclusions: This study aimed to design a study comparing the sedative effects of remimazolam and midazolam in gastrointestinal endoscopy in Japanese patients and to provide evidence that remimazolam is useful for sedation in the endoscopy field.
PP-01-121
Revisiting duodenal mucosal resurfacing in treatment of type 2 diabetes melllitus: An updated meta-analysis
Ahmad Fariz Malvi Zamzam Zein1,2, Ayu Oktaviana3, Edi Mulyana1, Nikko Darnindro1, Annela Manurung1, Marina Epriliawati4, Muhammad Ikhsan Mokoagow4, Jerry Nasarudin4 and Anak Agung Arie Widiastuti4
1Division of Gastroenterohepatology, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia;2Department of Internal Medicine, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;3Faculty of Medicine, Trisakti University, Jakarta, Indonesia;4Division of Endocrinology, Metabolism, and Diabetes, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This meta-analysis was performed to emphasize the benefit and safety of duodenal mucosal resurfacing (DMR) among patients with type 2 diabetes mellitus (T2DM) based on the most current evidence.
Materials and Methods: Cochrane Library, MEDLINE, Proquest, Pubmed, Scopus, and ScienceDirect were systematically searched reporting the outcome of DMR among patients with T2DM and published up to April 15th, 2024. Data extraction was performed independently by authors. The primary outcome was metabolic improvement including HbA1c, body weight (BW), and HOMA-IR. The secondary outcome was safety profile of DMR encompassing severe adverse event (SAE). Heterogeneity was examined using I² statistics and a random-effects model was employed. Review Manager 5.4.1 was used for statistical analysis.
Results: There were 7 studies recruiting a total 298 patients. All studies were prospective clinical studies. Six studies reported change in HbA1c in six months and one study reported at three months. There was significant HbA1c reduction in patients with DMR compared to baseline by 5.18% (95% CI -6.90, - 3.46, p <0.001). Additionally, DMR was associated with BW reduction by 0.28 kg (95% CI -0.47, -0.09, p <0.001) and decreased HOMA-IR by 0.91 (95% CI -1.19, -0.63). There were three out of 118 patients (2.54%) had SAE with an effect size of 1.67.
Conclusion: Performing DMR provides beneficial metabolic outcomes in management of T2DM. It is also a harmless endoscopic approach for patients with T2DM.
PP-01-122
Obscure bleeding from a metastatic small bowel tumor diagnosed using motorized spiral enteroscopy: case report
Christian Banciu and Andreea Munteanu
University of Medicine and Pharmacy TImisoara/IV Department of Internal Medicine, Timisoara, Romania
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Small bowel tumors are relatively rare, representing only around 5% of all gastrointestinal neoplasms, with a progressively increasing incidence. Currently, there are no established guidelines for diagnostic approaches, screening procedures, or management strategies for small bowel tumors.
Case desciption: We present here the case of a patient with a rare type of metastatic tumor of the small bowel originating from primary lung adenocarcinoma who presented with abdominal pain, severe iron-deficiency anemia, and melena. The initial investigations, gastroscopy and colonoscopy, failed to identify the bleeding source. The obscure bleeding source and diagnosis were achieved through power motorized spiral enteroscopy (MSE), which allowed the visualization and biopsy of the tumor. Histopathological examination established the presence of a poorly differentiated non-mucinous adenocarcinoma originating from the lung.
Discussion: although the MSE was abandoned world wide, we report this case to provide evidence of the efficiency of MSE in the diagnosis of small bowel tumors, with the method providing higher insertion depth in a reduced amount of time. In experienced hands we suggest that MSE still represents a safe and more time saving method than other types of enteroscopy, allowing to perform diagnostic and therapeutic procedures on the small bowel. Off course, further technical improvements can and must be obtain on this endoscopy method (MSE) in order to make it more safe and reliable.
PP-01-123
Clinical Usefulness and Acceptability of Small-bowel Capsule Endoscopy with Panoramic Imaging Compared with Axial Imaging
Issei Hirata, Akiyoshi Tsuboi, Shuya Shigenobu, Yuka Matsubara, Takeshi Takasago, Tomoyuki Nishimura, Hidenori Tanaka, Ken Yamashita, Yuichi Hiyama, Hidehiko Takigawa, Yuji Urabe, Toshio Kuwai and Shiro Oka
Hiroshima University Hospital, Hiroshima, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate the usefulness and acceptability of CapsoCam Plus® (CapsoCam).
Materials and Methods: This retrospective single-center study enrolled 930 patients with suspected small-bowel bleeding (SSBB) who underwent capsule endoscopy. Thirty-three patients using CapsoCam and PillCamTM SB3 (SB3) were matched using the propensity score matching. The diagnostic yield was evaluated by comparing both groups and the acceptability of CapsoCam use.
Results: There was no SSBB case where capsule endoscopy was performed within 48 h of bleeding. CapsoCam had a significantly higher observation rate of the entire small-bowel (97% vs. 73%, P = 0.006) and Vater’s papilla (82% vs. 15%, P < 0.001) than SB3. The reading time of CapsoCam was significantly longer than that of SB3 (30 vs. 25 min, P < 0.001), and CapsoCam’s time from the capsule endoscopy swallowing to read completion was also longer than that of SB3 (37 vs. 12 h, P < 0.001). The two groups showed no difference in the capsule endoscopy findings according to P classification. Notably, 85% of the patients using CapsoCam reported examination distress as "not at all" or "almost not," and 94% reported swallowing difficulty as "very easy" or "easy.”
Conclusion: CapsoCam took time to read; however, it is a well-tolerated examination with a high observation rate of Vater’s papilla and entire small-bowel mucosa. Detectability of bleeding sources was comparable in both modalities for cases of occult SSBB and overt SSBB more than 48 hours after bleeding. CapsoCam is a useful modality for patients with SSBB.
PP-01-124
Clinical Outcomes of Delayed Capsule Endoscopy in Inpatients with Small Bowel Bleeding:Propensity Score Matching Analysis
Seung Min Hong1, Dong Hoon Baek1, Cheolung Kim1, Hong Sub Lee2, Geun Am Song1, Hyeon Tae Cho3, Jeong Gil Park4, Gwang Ha Kim1, Bong Eun Lee1, Moon Won Lee1 and Dong Chan Joo1
1Department of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea;2Inje University Busan Paik Hospital, Busan, South Korea;3Centum Medihill Hospital, Busan, Republic of Korea;4Kimsan Internal Medicine Clinic, Sacheon, Republic of Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This retrospective study analyzes the clinical outcomes of performing delayed capsule endoscopy in patients hospitalized for small bowel bleeding.
Materials and Methods: All patients were divided into two groups: the Early-48 group (n = 46), who underwent capsule endoscopy within 48 hours of the bleeding episode, and the Late-48 group (n = 54), who underwent capsule endoscopy more than 48 hours after the bleeding episode. Using 1:1 propensity score matching (PSM) analyses, 34 pairs were made.
Results: After the 1:1 PSM, there was no difference in yield for both groups to detect positive findings on capsule endoscopy, the need for intensive care unit (ICU) care, the amount of blood transfusion, and the number of recurrent bleeding episodes (Table 1). When the patients were divided into two groups based on 72 hours and after performing 1:1 PSM, the group that underwent capsule endoscopy within 72 hours of a bleeding episode had a significantly higher yield in detecting positive findings compared to the group that underwent capsule endoscopy more than 72 hours after a bleeding episode. (95.5% vs. 68.2%, p = 0.046). However, there were no differences in other clinical outcomes between the two groups.
Conclusion: Although performing delayed capsule endoscopy on inpatients with small bowel bleeding may reduce the yield in detecting positive findings, it is considered feasible as it does not affect patient survival and the outcomes of inpatient treatment.
PP-01-125
Significance of Surveillance for Small bowel Tumor with balloon-assisted enteroscopy in Familial Adenomatous polyposis (FAP)
1Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan;2Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;3Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The pathophysiology of small bowel (SB) tumors in patients with familial adenomatous polyposis (FAP) remains unclear, and surveillance methods have not yet been established. Therefore, the purpose of this study was to investigate the significance of SB surveillance with balloon-assisted enteroscopy (BAE).
Materials and Methods: We analyzed the severity of SB tumors and their correlation with disease severity in other organs from 30 patients associated with FAP who underwent BAE for SB from October 2023 to June 2024. The severity of SB tumors was recorded every 30 cm from the Treitz ligament in the upper SB and from the ileocecal valve or the postoperative anastomosis in the lower SB.
Results: The mean age of the patients was 49 years, and 78% of the patients had undergone total colectomy. The average of insertion depth of trans-oral enteroscopy was 102 cm from Treitz ligament, and lesions were observed in 55% of the patients. The lesions were presented 48%/23%/19% in each part of the upper SB (30 cm/30-60 cm/60-90 cm from Treitz ligament). Large lesions of more than 15 mm were observed in 16% of cases. Trans-anal enteroscopy was performed in 7 patients, and lesions were observed in 13% of patients. No correlation was found between the presence or absence of upper SB lesions and the severity of gastric or duodenal polyposis.
Conclusion: SB surveillance with BAE should be performed in patients with FAP, regardless of the severity of other gastrointestinal tumors.
PP-01-126
Evaluating the role of small intestine dual energy CT and double-balloon endoscopy in Crohn’s disease
Yanjun Chen, Ji Liu and Fujuan Luan
The First Affiliated Hospital Of Soochow University, Suzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study seeks to evaluate the role of small intestine DECT and DBE in the diagnosis of CD.
Materials and Methods: The study included 72 CD patients (4 patients underwent both DECT and DBE twice, totally, 76 CD patients’ data) who underwent both small intestine DECT and DBE within 3 months and were hospitalized in the First Affiliated Hospital of Soochow University from July 1, 2016, to November 1, 2023.
Results: Among 76 CD patients’ data, there were 51 males and 25 females, with an age of (38.86 ± 12.05) years old. CD detection rate by DBE was 80.3%, higher than 65.8% by DECT (P = 0.044). The combined CD detection rate was 89.5%, higher than DECT alone (P < 0.05). The detection rate of stenosis by DBE was 46.1%, higher than 13.2% by DECT (P < 0.05). The combined detection rate of stenosis was 52.6%, higher than DECT alone (P < 0.05). The detection rate of fistula by DBE was 3.9%, by DECT was 2.6%, and by the combined method was 5.3%. For ulcers, DBE had a higher detection rate of 73.7% compared to 7.9% of DECT (P < 0.05). The combined ulcer detection rate was 76.3%, higher than DECT alone (P < 0.05). The detection rate of long ulcers and non-ileocecal ulcers by DBE were both 17.9%.
Conclusion: For the detection of CD, stenosis, and ulcers, DBE or combined method has an advantage over DECT. Notably, DBE has an advantage in detecting long ulcers and non-ileocecal ulcers.
PP-01-127
Clinical case: Small intestinal capsule endoscopy and histopathology of Patients with Eosinophilic Enteritis
Tiến sĩ Phu Pham Quang1, Thuan Nghiem Duc2, Thuong Nguyen Hoai1, Nhuong Duong Xuan1, Hong Nguyen Phuong1, Chi Dinh Minh1, Chung Le Ngoc1, Huong Huynh Thi Thu1, Lai Nguyen Van1, Linh Nguyen Thuy1, Thanh Thanh Nguyen Hong1 and Kim Nguyen Huu1
1Military Hospital 103, Hanoi, Vietnam;2Vietnam Military Medical University, Hanoi, Vietnam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Eosinophilic gastroenteritis (EGE), which demonstrates pathological dense eosinophil infiltration in the gastrointestinal tract irrespective of esophageal involvement. We would like to report a clinical case of eosinophilic enteritis with capsule endoscopy and histopathology.
Case Description: 20-year-old male presented with fatigue, anorexia, abdominal pain. Hemoglobin level, 113 g/L; mean corpuscular volume, 76,7 fL), and peripheral eosinophilia (665 cells/mL). Stool examinations were negative for parasites. The diagnosis of tuberculosis infection was ruled out by the quantiferon test and on the biopsy.
Upper endoscopy were grossly and histologically unremarkable. Colonoscopy showed nothing unremarkable histologically and grossly in the colon. Inserting the scope through the Bauhin valve into the ileum detects erosive inflammation and ulceration. A biopsy of the lesion is performed showed jejunal eosinophilia with over 50 eosinophils per high-power field. (Figure A)
The image of the lesion detected through capsule endoscopy continuously from the capsule location at 3 hours 35 minutes to the end of the ileum at 9 hours 4 minutes. Redness or erythema was the most common endoscopic finding in the small bowel, followed by villous atrophy, erosion, ulceration, and edema. Other findings included mucosal congestion, whitish exudate and short-rounded edematous villi. (Figure B)
Discussion: The characteristics of small bowel endoscopic findings on EGE remain unclear. It is hoped that this report will provide a valuable source for further clarification of the endoscopic findings of the small bowel and further elucidation of the pathogenesis of EGE through more detailed studies with a larger number of cases.
PP-01-128
Per-oral Endoscopic Myotomy (Z-POEM) As An Effective Treatment for Zenker's Diverticulum: A Case Report
Saskia Nursyirwan and Achmad Fauzi
Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Zenker's diverticulum (ZD) arises from impaired cricopharyngeal (CP) muscle distensibility during deglutition (swallowing), leading to a clinical presentation characterised by dysphagia, regurgitation, aspiration, cough, and potential weight loss. The advent of flexible endoscopic ZD treatment has revolutionized the therapeutic landscape, rapidly becoming the preferred first-line modality for management of small to moderate-sized diverticula.
Case Presentation: In this medical illustration, we report a woman, 64th years old with Zenker’s diverticulum treated with Z-POEM. She came to our clinic with dysphagia and regurgitation since 6 months prior. Diagnostic gastroscopy showed ZD of 3 cm, located 18 cm from incisors with thick septal muscle. A triangle-shaped knife created a 2-cm mucosal incision, and submucosal tunneling was made by spray coagulation. The gastroscope was advanced through the submucosal space of the esophageal lumen and the diverticulum site until the bottom of the diverticulum. The septal muscle was completely cut, immediately allowing the gastroscope to pass through easily, and the mucosal defect was closed with hemoclips.
Discussion: Z-POEM offers a precise approach to treating Zenker's diverticulum by providing a complete visualization of the entire septal muscle. This comprehensive view minimizes the risk of incomplete myotomy, ensuring a more effective treatment.
Association between the symptoms and the UGIE findings in a cohort of patients
Nirodha Abeywardhana, Sanka Abeynayake and Chaminda Rajakaruna
Base hospital Gampola, Kandy, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Upper gastrointestinal endoscopy (UGIE) is a diagnostic as well as a therapeutic endoscopic procedure which is used to visualize from oropharynx to the 2nd part of the duodenum. It is commonly done to evaluate upper GI symptoms. This study was done to describe the association between the symptoms and the findings.
Materials and Methods: Cross sectional descriptive study was conducted at Base Hospital Gampola, Sri Lanka using patients who had undergone UGIE over a six months period. Findings were recorded and analyzed using standard software.
Results: 152 patients were in the sample. Mean age was 59+/-15.3 years. 86 (57%) were females. The most common symptom was dyspeptic symptoms 70 (46%), followed by dysphagia (24%), upper GI bleeding 23 (15%). Most common findings were gastritis 40 (26%) and hiatal hernia 22 (15%). Dyspeptic symptoms were associated with 37% gastritis, 22% hiatal hernia and 5% gastric masses. Dysphagia is 22% associated with esophageal masses, 16% hiatal hernia and 11% gastritis. Eleven percent of patients with dyspeptic symptoms and 16% of patients with dysphagia had normal studies. Six patients (4%) had gastric masses and 8 patients (5%) had oesophageal masses. Half of the patients with oesophageal masses and 1/3 of patients with gastric masses are from Tamil population. 16% of Tamil patients who had undergone UGIE had malignant lesions (p=0.1).
Conclusions: The Symptoms dyspepsia, dysphagia and Upper GI bleeding have significant association with UGIE findings. Being a Tamil increases the risk of having an upper GI malignancy
PP-01-130
The Association between Drug Induced Gastropathy and Endoscopic Gastroduodenal Mucosal Lesions
Abida Abida, Budi Widodo, Titong Sugihartono and Herry Purbayu
Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Drug induced gastropathy (DIG) is lesions of the gastric mucosa asscociated with negative manifestation either of drug or its metabolites as NSAIDs, aspirin, anticoagulants, glucocorticosteroids, anticancer drugs and oral iron preparations. The aim of this study to determine association between DIG and Endoscopic Gastroduodenal (EGD) mucosal lesions in patiens using NSAIDs, Glucocorticosteroids, Single / Double Antiplatelets (SAPT/DAPT) and Herbal Medicine.
Material and Methods: The Study was a Cross-sectional Study conducted in Dr. Soetomo General Academic Hospital Surabaya period 2023-2024. Total 99 Subjects with dyspepsia symptoms were enrolled, medications history, EGD and histopathological examination were performed. Gastric mucosal erosion and ulcer were classified using modified LANZA Score. We analyzed data with CHI-SQUARE Test and p value < 0.05 was statistically significant.
Results: EGD mucosal lesions were observed in 62 patients (63.6%) and 37 patients (37.4%) had ulcers. The modified Lanza Score Mean ±SD was 3.39 ±1.5. There were statistically significant association among Single Therapy (NSAID/ SAPT/ Corticosteroids), Dual Therapy (NSAID+Corticosteroids/DAPT) and Triple Therapy (NSAID + Corticosteroids + Herbal Medicine/NSAID+DAPT) with EGD mucosal lesions (p value = 0.0001, p value = 0.0001 and p value = 0.003 respectively). Duration of DIG administration, alcohol habit, severity subjective symptoms were contributing factors associated with EGD mucosal lesions showed statistically significant (p value = 0.0001, p value= 0.0001, p value = 0.001 respectively).
Conclusion: Our finding suggested that Single Therapy, Dual Therapy, Triple Therapy, duration of DIG administration, Alcohol habit and severity subjective symptoms were contributing factors associated with EGD mucosal lesions.
PP-01-131
Duodenal tumor finding in patient with renal carcinoma
Budiman Syaeful Anwar and Muhammad Firhat Idrus
RSCM, Central Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Renal cell carcinoma (RCC) is the most common malignant tumor of the kidney and constitutes over 90 percent of all renal malignancies. A third of the patients with RCC have metastatic disease at presentation1. Duodenal tumor is a rare case in patient with renal RCC. Most patients of duodenal metastasis from RCC present with upper gastrointestinal bleeding or obstructive symptoms, and other signs include anemia, melena, fatigue and early satiety2. Imaging remains the mainstay in RCC for diagnosis, screening, follow-up, and treatment monitoring1. Diagnosis of duodenal metastases as a cause of GI bleeding is a challenge due to its rarity and thus low index of suspicion for diagnosis3.
Case Report: A 51-year-old male came with heavy weakness. 7 days before hospital admission patient had hematuria with blood clots. Patient diagnosed with renal tumor in 2018, but have not done any surgery due to economic and distance to referral hospital. This patient just performed a renal biopsy around two weeks ago. Patient was consulted to Gastroenterology because of melena since two days ago with hemoglobin levels only 5,5 g/dL. History of hematemesis and hematochezia was denied. EGD results revealed a duodenal mass pars d1-d2 junction.
Conclusion: Patient with renal carcinoma who experienced digestive bleeding, iron deficiency anemia, a mass in the GI tract, or a history of RCC has possibility of gastrointestinal metastasis. Therefore, the patient should be suspected and get complete evaluation, such as biopsy and abdominal CT scan with contras can be option to confirm the definite diagnosis.
PP-01-132
Factors Influencing Endoscopic Treatment of Children with Esophageal Atresia after Esophagoplasty
Dmitriy Baranov, Suhotskaya Anna, Valeria Kamalova, Malysheva Daria, German Goryaev, Evgeniy Solonitsyn, Vladislav Tutunnik, Sabina Seifedinova, Dmitriy Chernyh and Anna Poddymova
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Factors Influencing Endoscopic Treatment of Children with Esophageal Atresia after Esophagoplasty.
Introduction: The aim of this study was to investigate various aspects of treating patients with a particular pathology, including gender differences, the impact of the type of surgery, and the presence of tracheoesophageal fistula (TEF) on the duration of bougienage and treatment outcomes.
Case Description: The study included 18 childrens (9 male and 8 female). The average duration of bougienage was 10 months, with an average of 7 sessions. Both single-stage (n = 12) and multi-stage surgeries (n = 6) were performed. The mortality rate for surgical treatment was 5%. The diameter of the stricture ranged from 2.5 mm to 7.3 mm. TEF was present in 4 out of 18 patients (78%).
Statistical analysis revealed significant differences in the duration of bougienage between male and female patients (p = 0,006, Student's t-test). Males required longer treatment.
There were no statistically significant differences in bougienage duration between patients undergoing single-stage and multi-stage surgeries (p = 0,820, Mann-Whitney U-test).
No correlation was found between the initial stricture diameter and the duration of bougienage.
The presence of TEF did not significantly affect the duration of bougienage.
Discussion: The study identified gender differences in the duration of bougienage, with males requiring longer treatment. However, the type of surgery did not significantly influence the treatment duration. Further research is needed to better understand the impact of various factors on treatment outcomes.
PP-01-133
Injectable pH-responsive bioadhesive with robust and stable wet adhesion for gastric ulcer healing
Dake Chu
Xian Jiaotong University, Xi'an, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Development of bioadhesives that can be facilely delivered by endoscope and exhibit instant and robust adhesion with gastric tissues to promote artificial gastric ulcer healing remains challenging.
Materials and Methods: In this study, an advanced bioadhesive is prepared through free radical polymerization of ionized N-acryloyl phenylalanine (iAPA) and N-[tris (hydroxymethyl) methyl] acrylamide (THMA).
Results: The precursory polymer solution exhibits low viscosity with the capability for endoscope delivery, and the hydrophilic-hydrophobic transition of iAPA upon exposure to gastric acid can trigger gelation through phenyl groups assisted multiple hydrogen bonds formation and repel water molecules on tissue surface to establish favorable environment for interfacial interactions between THMA and functional groups on tissues. The in-situ formed hydrogel features excellent stability in acid environment (14 days) and exhibits firm wet adhesion to gastric tissue (33.4 kPa), which can efficiently protect the wound from the stimulation of gastric acid and pepsin. In vivo studies reveal that the bioadhesive can accelerate the healing of ulcers by inhibiting inflammation and promoting capillary formation in the acetic acid-induced gastric ulcer model in rats.
Conclusion: Our work may provide an effective solution for the treatment of gastric ulcers or artificial ulcers via endoscopic injection clinically.
PP-01-134
Efficacy of draid endo in detecting upper gastrointestinal tract landmarks and lesions on endoscopy videos
Viet Hang Dao and Duc Tran and Hoang Nguyen and Kien Dao and Giap Duong and Binh Nguyen and Tung Nguyen and Hoa Lam and Trang Nguyen and Steven Truong and Long Dao
Hanoi Medical University, Hanoi, Viet Nam;Institute of Gastroenterology and Hepatology, Hanoi, Vietnam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The application of artificial intelligence (AI) in endoscopy is increasing, especially in developing commercialized products for real-time lesions detection. However, data in developing countries is limited.
Objectives: Evaluate the accuracy of the DrAid Endo model in detecting anatomical landmarks and lesions on upper gastrointestinal (UGI) endoscopy images and videos.
Methods: DrAid Endo's algorithm was trained on a dataset comprising 46,524 endoscopic images without lesions and 13,342 endoscopic images of five lesions: reflux esophagitis, esophageal cancer, gastritis, gastric cancer, and duodenal ulcer. The dataset includes four light modes: white light and 3 image-enhanced modes (FICE, LCI, BLI). All images were labeled and verified by endoscopists with ≥5 years of experience. The EfficientNet-B5 model was used for anatomical landmark detection and YOLOv8 model for lesions detection. Accuracy of the DrAid Endo model was evaluated on 2583 images for UGI anatomical landmark detection and 47 endoscopic videos for UGI lesions detection. The endoscopic videos were divided into 284 positive sections (containing lesions) and 296 negative sections (containing no lesions) with a total time of 87.55 minutes and 75.23 minutes, respectively.
Results: The anatomical landmark detection algorithm achieved 95% sensitivity and 99% specificity. As for lesions detection, sensitivity and specificity ranged from 91% to 96% for most lesions, except for gastritis. Sensitivity and specificity were highest in gastric cancer (95.6%, 95.9%, respectively) and lowest in gastritis (65.3%, 73.9%, respectively).
Conclusion: DrAid Endo shows efficacy in detecting anatomical landmarks and some UGI lesions, especially malignant lesions.
PP-01-135
Hookworm infection presenting as acute massive upper gastrointestinal bleeding : A case report
Aryanti Ambarsari and Muhamad Yugo Hario Sakti Dua
Gastroenterology Hepatology RSUD Provinsi Banten, Serang, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Chronic occult bleeding is well known in cases of hookworm infection, but acute gastrointestinal bleeding is rarely reported.1 A challenging evaluation is needed since soil-transmitted helminths are among the neglected tropical diseases.2 Hookworm infection is a curable condition and has a good prognosis of complete recovery.4
Case: A 53-years old male, presented to ER with multiple large episodes of melena since one day prior admission. Hemoglobin dropped to 3,2 mg/dL from the previous 6,4 mg/dL. He was in hypovolemic shock, thus he admitted to the ICU and kept on an intravenous PPI and somatostatin. An upper GI endoscopy is performed after he received a 5-unit of blood transfusion. It revealed esophagitis LA grade B, antrum gastritis with multiple ulcer Forrest III, and duodenitis with an adult worm in the duodenum. The worm was removed, then 400 mg of albendazole were administered once daily for the first five days. No further massive bleeding was observed and he was stable clinically and hemodynamically.
Discussion: An evaluation of duodenum using an endoscopy result in a more frequent diagnosis of worm infection as a cause of acute gastrointestinal bleeding.3 The multiple ulceration seen on endoscopy as the mechanical trauma of the intestinal mucosa due to the worms’ attachment and abrasion. This was contributed to blood loss in the absence of NSAID consumption. Hookworm infection that manifests clinically as acute digestive hemorrhage is infrequent. In tropical country, it is important to consider this pathology within the differential diagnosis in cases of bleeding.
PP-01-136
Photodocumentation protocol for UGI endoscopy as the Quality Indicator in the National Cancer Screening Program
Kseniya Filimendikova, Sergey Kashin and Anastasiia Anastasiia
Yaroslavl Regional Cancer Hospital, Yaroslavl, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Esophageal and gastric cancer occupies one of the leading places in the structure of cancer morbidity (1,24 % and 5,3%, in 2022, respectively) and mortality (2,28% and 8,8% in 2022, respectively) in Russia. The use of a standardized photodocumentation protocol for EGD can help reduce mortality and increase the diagnosis of precancerous lesions, early esophageal and gastric cancer, and other conditions.
Materials and Methods: A cohort of 211 random patients, who underwent an endoscopic examination, (66% female, 34% male), average age 59,6 years (18-84), diagnosed in 2023 in a referral center, was analyzed. The number of photos taken during the study, the time taken to perform the EGD were calculated and also the concordance of the referring institution's diagnosis with the study result was evaluated.
Results: Average number of photographs in the protocol - 9, average time of EGD - 5.8 minutes. The number of matches between the referring institution's diagnosis and the endoscopic examination reports – 27%. The number of diagnoses, that did not match – 31,8%. The number of cases where the data cannot be evaluated — 41,2%.
Conclusion: The data presented demonstrate the need for a photodocumentation protocol for quality control of endoscopic examination.
PP-01-137
Clinically significant endoscopic findings in outpatients with dyspepsia without alarm features
Taufik Hidayat1, Putut Bayupurnama2, Neneng Ratnasari2, Fahmi Indrarti2 and Catharina Triwikatmani2
1Trainee in Division of Gastroenterology and Hepatology, Internal Medicine Department, Faculty of Medicine, Public Health and Nursing Gadjah Mada University; Dr. Sardjito Hospital, Yogyakarta, Indonesia;2Division of Gastroenterology and Hepatology, Internal Medicine Department, Faculty of Medicine, Public Health and Nursing Gadjah Mada University; Dr. Sardjito Hospital, Yogyakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Some guidelines recommend a non-endoscopic approach in dyspepsia patients without alarm features, but several studies have shown significant endoscopic findings (SEF) in this group of patients with varying prevalence.
Objective: This study aims to determine the prevalence and factors associated with SEF in outpatients with dyspepsia without alarm features.
Material and Methods: We did retrospective study for outpatients with dyspepsia without alarm features who underwent endoscopy at Sardjito General Hospital Yogyakarta, between January 2023-April 2024. Demographic, symptom, BMI, and endoscopy data were collected from medical records. We defined SEF as peptic ulcer, erosive esophagitis (LA grade B and higher), malignancy, stricture, or findings required specific therapy.
Results: A total of 110 outpatients with dyspepsia without alarm features were included in this study. The median age of participants was 43 (18-72), male 66 (60%), female 44 (40%). We had SEF in 21 (19.1%) of which peptic ulcer was 16, hiatal hernia was 5, erosive esophagitis LA grade B was 2 patients. The ≥ 50 years was statistically associated with SEF (OR = 3,196; p=0.033).
Conclusion: The prevalence of SEF in outpatients with dyspepsia without alarm features was 19%, with peptic ulcer being the most common finding. The ≥ 50 years are associated with higher risk of SEF.
Successful closure with Four Tracheal and Esophageal Stents for Esophagobronchial Fistula caused by Esophageal Cancer
Ryusuke Kato1, Yohei Yamamoto1, Masamitsu Sotozono1 and Miku Sato2
1Sapporo Teishinkai Hospital, Sapporo-shi, Japan;2Obihiro Kousei Hospital, Obihiro-shi, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Esophagobronchial fistulas caused by esophageal cancer in the cervical esophagus are notoriously refractory, presenting significant treatment challenges.
Case Description: A man in his 50s was diagnosed with stage IVa esophageal cancer with tracheal invasion and cervical lymph node metastasis. Despite chemoradiotherapy and systemic chemotherapy with re-irradiation, disease progression resulted in an esophagobronchial fistula, necessitating the placement of two airway stents. One month later, esophageal stenosis occurred due to compression from the airway stents, requiring an additional esophageal stent. A fully covered self-expandable metal stent with short flares, designed for the cervical esophagus, was placed. The patient maintained a stable condition for nearly six months, but the progression of the esophageal cancer led to the expansion of the esophagobronchial fistula, necessitating additional airway stents. Ultimately, four stents were used, allowing the patient to maintain quality of life for nearly a year despite the high-risk location and persistent stent treatment challenges in the cervical esophagus.
Discussion: Despite the refractory nature of the esophagobronchial fistula due to esophageal cancer progression, persistent stent treatment proved to be a viable therapeutic option. The high-risk location in the cervical esophagus typically poses a risk for reduced quality of life, yet this patient maintained a relatively stable condition and quality of life for an extended period. This case underscores the potential benefits of aggressive stent therapy in managing complex esophageal cancer complications, even in high-risk anatomical regions.
PP-01-139
ASD Closure Devices: An Effective Way of Managing Leakage Following Laparoscopic Sleeve Gastrectomy
Jayamini Kaushalya, Anjana Abayasinghe, Samitha Senevirathne and Ishan De Zoysa and Nilesh Fernandopulle
Professorial Surgical Unit, National Hospital, Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Obesity is a major healthcare burden. Laparoscopic sleeve gastrectomy (LSG) is a widely used bariatric procedure that achieves substantial weight loss and improves obesity-related comorbidities. Even though rare, leakages from the stapler line occur in 1–3% of patients, posing a severe complication. Such leaks can lead to serious, life-threatening consequences if not managed promptly and effectively.
Case report: A 58-year-old female with grade 2 obesity, complicated by metabolic syndrome, obstructive sleep apnea, and bilateral knee osteoarthritis, underwent LSG. Post-operatively, on day 15, she was diagnosed with a leakage from the stapler line at the gastric fundic region. Initial management involved endoscopic clipping of the defects and placement of a gastric mega stent. Following the failure of those interventions, subsequently, endoscopic application of two ASD (Atrial Septal Defect) closure devices was employed to manage the leak. This approach proved successful, effectively sealing the defect and allowing for resolution of the leakage.
Discussion: Management of leaks following LSG requires a tailored approach based on the patient's hemodynamic stability and the extent of the leak. Initial management strategies involve patient resuscitation, followed by endoscopic evaluation and interventions such as stent placement, clipping, or endoluminal wound vacuum. This case demonstrates the potential of repurposing ASD closure devices in managing post-LSG leaks. While the literature on this application is limited, it offers a promising alternative in the failure of conventional treatments. Further, this highlights the importance of innovative approaches to complex surgical complications.
PP-01-140
Endoscopic resection for low grade duodenal neuroendocrine tumors: A single center-retrospective analysis
Jin Ook Jang, Cheol Woong Choi, Eun Jeung Choi, Su Bin Sin and Cheol Min Lee and Woojin Kim
Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan-si, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: High resolution videoendoscopic system and increased number of screening endoscopic examination enable us to detect incidental small duodenal neuroendocrine tumor (D-NET). The selection between radical surgical resection and local excision, including endoscopic resection for D-NET remains controversial. Recent guidelines recommend endoscopic resection for D-NET < 10 mm. Considering recent advancements in therapeutic endoscopic procedures, we aimed to evaluate the feasibility of diagnostic endoscopic resection of D-NETs grade 1 or 2.
Methods: We retrospectively analyzed forty D-NETs diagnosed with grade 1 or 2 at a single tertiary referral center between January 2009 and July 2023.
Results: The mean follow-up period was 59.0 ± 6.8 months. The mean NET size was 7.4 ± 0.8 mm, and most patients’ NETs were <10 mm (80.0%). The maximal NET diameter of NETs was 25 mm. During the study period, most NETs were grade 1 (n=36, 90%). Only 17.5% of patients were treated surgically at first. After endoscopic treatment, 2 additional surgical resection were performed, which revealed no evidence of lymph node metastasis. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Among endoscopic resections, ligation assisted endoscopic mucosal resection (EMR) showed a 100% complete resection rate.
Conclusions: We observed that small-sized D-NETs grade 1 and 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection could be recommended for D-SETs with grade 1 or 2. Ligation assisted EMR is preferred for less than 10 mm in size.
PP-01-141
The endoscopic resection for low grade gastric neuroendocrine tumors: A retrospective analysis
Won Jun Jang, Cheol Woong Choi, Eun Jeung Choi, Woojin Kim and Su Bin Sin
Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan-si, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The recent surge in screening endoscopy has led to increased detection of gastric neuroendocrine tumors (G-NETs). The selection between radical surgical resection and local excision, including endoscopic resection, for the management of G-NETs remains controversial. Currently, endoscopic resection is recommended for G-NETs < 10 mm. We aimed to evaluate the feasibility of diagnostic endoscopic resection of G-NETs.
Methods: We retrospectively analyzed 31 patients diagnosed with grade 1 or 2 G-NETs at a single tertiary referral center between January 2009 and December 2023. Outcomes, including histopathology, complete resection, and metastasis rates, were analyzed.
Results: The mean follow-up period was 38.9 ± 38.4 months. The mean size of G-NET was 4.9 ± 3.4 mm, and most patients’ NETs were <10 mm (87.1%). The maximal NET diameter of NETs was 16 mm. During the study period, most NETs were grade 1 (type 1: 90.9%; type 3: 85.0%). R1 resection patients (19.4%) showed no evidence of metastasis during follow-up without additional surgical management. None of the enrolled patients showed any evidence of lymph node metastasis or local recurrence. Recurrent or multiple G-NETs were observed only in the patients with type 1 NETs (27.2%, 3/11). Modified endoscopic mucosal resection (EMR) (precut, ligation assisted, cap-assisted, and underwater), and endoscopic submucosal dissection (ESD) showed a 100% complete resection rate.
Conclusions: We observed that G-NETs 1 or 2 had no lymph nodes or distant metastases. Diagnostic endoscopic resection is recommended for gastric SETs ≤16 mm in size. Modified EMR or ESD is preferred to conventional EMR.
PP-01-142
Characteristics and predictive scoring system for hemorrhagic duodenal ulcer with difficult endoscopic hemostasis
Saiseikai Matsusaka General Hospital, Matsusaka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To clarify the characteristics of hemorrhagic duodenal ulcer (HDU) with difficult endoscopic hemostasis and whether existing scoring systems can be used to predict difficult-to-treat cases.
Materials and Methods: Between January 2008 to June 2024, we retrospectively enrolled 91 patients. HDU was defined as ulcer that required some kind of hemostatic treatments. <Study1> We classified the patients into two groups, group A (GA), difficult endoscopic hemostasis) and group B (GB), successful hemostasis, and compared the patient backgrounds, ulcer locations and findings. <Study2> We evaluated which scoring systems for upper gastrointestinal bleeding (Glasgow-Blatchford Score (GBS), AIMS65 score, clinical Rockall score (CRS)) can predict difficult endoscopic hemostasis using the area under the ROC curve (AUC).
Results: <Study1> GA had 12 cases, and GB had 79 cases. History of duodenal ulcer was more common in GA than in GB (55 vs 19%, p=0.01). There were no differences in age, gender, NSAIDs/antithrombotic medication or ulcer locations. At the time of bleeding, GA had significantly lower systolic blood pressure compared with GB(93 vs 110mmHg, p=0.04), lower hemoglobin(6.0 vs 8.2 g/dl, p=0.03), lower albumin(2.5 vs 3.3 g/dl, p=0.02)and prolonged PT-INR(1.39 vs 1.13, p=0.007). <Study2> AUC [95%CI]: GBS 0.75[0.58-0.90]/AIMS65 0.80[0.64-0.95]/CRS 0.73[0.60-0.85]. AIMS65 was most useful, and the cutoff value for endoscopic hemostatic difficulty was score 3. All cases with AIMS65 score 0 had endoscopic hemostasis.
Conclusion: HDU with hypotension, severe anemia, hypoalbuminemia, and prolonged PT-INR had difficulty with endoscopic hemostasis. AIMS65 was useful in predicting HDU with difficult endoscopic hemostasis.
PP-01-143
Comparison of EUS-FNB and Artificial Intelligence-based EUS Imaging in the Diagnosis of Subepithelial Lesions <20mm
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Although Endoscopic ultrasound fine-needle biopsy (EUS-FNB) is the gold standard for diagnosing gastric subepithelial lesions (SELs), its accuracy is low for SELs <20mm. With advancements in endoscopic techniques allowing for full-thickness resection of SELs, accurate and simple diagnosis of small SELs could offer significant healthcare benefits. Therefore, leveraging rapidly developing artificial intelligence (AI) technology, we created an AI system for gastric SELs (referred to as EUS-AI) and compared its diagnostic accuracy with EUS-FNB for SELs < 20mm.
Materials and Methods: From April 2010 to March 2020, we evaluated the diagnostic accuracy of EUS-AI using 250 cases of gastric SELs assessed by EUS and pathologically confirmed via EUS-FNAB/surgical intervention. We trained the AI using a deep learning system with neural networks on EUS images of SELs. For validation, we trimmed EUS images of 30 consecutive cases with gastric SELs under 20mm to include only the lesion areas and had AI diagnose these, comparing it to EUS-FNB. Diagnostic capabilities were measured by accuracy, sensitivity, and specificity for GIST/non-GIST.
Results: EUS-FNB had a tissue acquisition rate of 66.7% for gastric SELs under 20mm, with an accuracy of 66.7%, sensitivity of 66.7%, and specificity of 100%. EUS-AI achieved an accuracy of 76.7%, sensitivity of 78.6%, and specificity of 75%. There were 11 cases where EUS-FNB could not obtain tissue, and EUS-AI correctly diagnosed 82% of these.
Conclusion: EUS-AI demonstrated comparable diagnostic accuracy to EUS-FNB for lesions under 20mm and could potentially aid future clinical practices.
PP-01-144
Impact of intra-tumoral injection of Phosphorus-32 microparticles on vascularity in locally advanced pancreatic carcinoma
Amanda Lim1,2, Joshua Zobel1, Madison Bills4, William Hsieh4, Benjamin Crouch4, Rohit Joshi5, John-Edwin Thomson6, Eu Ling Neo6, Li Lian Kuan6, Ms Romina Safaeian1, Edmund Tse1,2, Christopher Rayner1,2, Andrew Ruszkiewicz2,8,9, Singhal Nimit3, Dylan Bartholomeusz1,4 and Nam Nguyen1,2
1Gastroenterology and Hepatology, Royal Adelaide Hospital, Adelaide, Australia;2The University of Adelaide, Adelaide, Australia;3Department of Oncology, Royal Adelaide Hospital, Adelaide, Australia;4Department of Nuclear Medicine, Royal Adelaide Hospital, Adelaide, Australia;5Department of Oncology, Lyell McEwin Hospital, Adelaide, Australia;6Department of Hepatobiliary Surgery, Royal Adelaide Hospital, Adelaide, Australia;7Department of Upper Gastrointestinal Surgery, Queen Elizabeth Hospital, Adelaide, Australia;8Surgical Pathology, SA Pathology, Adelaide, Australia;9Centre of Cancer Biology, University of South Australia, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Dense stroma and poor vascularity are potential causes for chemo-resistance in pancreatic cancer. Gemcitabine/Nab-Paclitaxel chemotherapy has no impact on vascularity. This study aimed to assess vascularity changes within pancreatic tumours following implantation of 32P microparticles (OncoSil) in addition to standard chemotherapy.
Materials and Methods: Clinico-pathological outcomes and contrast-enhanced harmonic EUS (CH-EUS) was prospectively assessed in locally advanced pancreatic cancer (LAPC) patients who received OncoSil and FOLFIRINOX chemotherapy. CH-EUS were performed before, 4 weeks and 12 weeks after OncoSil implantation. Time intensity curve (TIC) and peak intensity analyses were markers of microvascular flow and reflect the vascularity of the mass lesion.
Results: 20 patients were recruited, with 15 completing 12-week follow-up. OncoSil implantation was successful in all patients with no procedural complications. At 12 weeks, median tumour size was significantly reduced (32mm to 24mm; p<0.001), with 5 patients (25%) had tumour downstaging for resections. There was significant intensity gain of contrast enhancement within the tumour after 4 and 12 weeks of OncoSil-implantation (baseline = 32.15 (IQR 18.08-54.35) vs.46.85 (IQR 35.05-76.6; p=0.007) vs. 66.3 (IQR 54.7-76.3; p=0.001, respectively). Estimated median survival was 16.0 months, with 80% of patients still alive and only 3/20 (15%) demonstrating both local- and distant-disease progression.
Conclusion: OncoSil implantation in addition to chemotherapy is not only associated with promising clinic-pathological outcomes, but also increased vascularity within pancreatic cancer. These findings suggest that potential impact of OncoSil on the tumour micro-environment and allow more delivery of chemotherapy agents to the cancer. A larger comparative trial is warranted.
PP-01-145
Endoscopic removal of foreign bodies from the gastrointestinal tract in children
Stanislaw Pieczarkowski, Kinga Kowalska-Duplaga, Malgorzata Sladek, Zofia Grzenda-Adamek, Bartosz Bogusz, Dariusz Chmiel and Krzysztof Fyderek
University Childrens Hospital Jagiellonian University Faculty of Medicine, Krakow, Poland
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Aim: Assessment of factors that may affect the success of the procedure.
Material and methods: Between 2012 and 2024, Ingested foreign bodies (IFB) was diagnosed in 171 children, 87 boys and 84 girls aged 0.4 to 16 years.
The patients were divided into two groups: Group 1 (128patients) - in which the removal of IFB with the first tool used was effective and group 2 (43 patients) - in which it was NOT effective. Both groups were analyzed in relation to the location of the IFB, the type of IFB and the tool used.
Results: The most frequently removed IFB were coins (n = 84; including 68 in group 1 and 16 in group 2) and button batteries (n = 19, including 15 in group 1 and 4 in group 2). There were no statistically significant differences between the study groups in terms of children's age, gender, time since IFB, or endoscopist. The effectiveness of the first tool used was 100% for magnets, 80.95% for coins, 79% for button batteries. The following were also analyzed: dependence between time from IFB and the number of tools used (p=0.8514), dependence between location of IFB and the number of tools used, (p=0.0541).
Conclusions: For coins, rat teeth are the most effective tool, while for button batteries and other types of IFBs, a Roth net is more useful. The problem is the type of IFB and its location, not the instrument used, the age, gender of the child or the endoscopist performing the procedure
PP-01-146
Differences in monocyte to lymphocyte ratio levels in gastritis and peptic ulcer cases
Tatag Primiawan
Gadjah Mada University, Magelang, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Peptic ulcers are abnormalities that are often found on endoscopic examination. The monocyte lymphocyte ratio examination is a simple examination that can be used to determine the prognosis of upper gastrointestinal disorders.
The aim of this study was to compare Monocyte-to-lymphocyte ratio (MLR) between gastritis patients and peptic ulcer patients.
Method: We carried out a retrospective analysis of patients who underwent endoscopy from July 2023 to January 2024. Demographic data, endoscopy results, laboratory parameters were taken from medical records. The monocyte to lymphocyte ratio was analyzed using statistical analysis.
Result: Based on endoscopy results, a total of 94 patients were included in this study, with 69 (73.4%) diagnosed with gastritis and 25 (26.6%) with peptic ulcers. The cohort comprised 40 (42.6%) males and 54 (57.4%) females.
The median MLR for gastritis patients was 0,3 (range 0,1 – 0,7), whereas for peptic ulcer patients, it was 0,28 (Range 0,13 – 0,79). A statistically significant difference in MLR was observed between patients with gastritis and those with peptic ulcers (p=0.022).
The ROC curve analysis identified a cut-off value of 0,37 forMLR. The odds ratio for the association between MLR and the occurrence of peptic ulcers is 8,14.
Conclusion: Our study revealed a statistically significant difference in (MLR) between patients with gastritis and those with peptic ulcers. The MLR value Lower than 0,37 is a significant risk factor for peptic ulcer.But this difference was not at a level that could be used in clinical practice.
PP-01-147
Endoscopic Hemostasis Procedures in Non Variceal upper gastrointestinal Hemorrhage at Jakarta Tertiary hospital
Tri Ferry Rachmatullah and Hasan Maulahela
Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Non variceal upper gastroinstestinal hemorrhage (NVUGIH) is a common gastrointestinal condition encountered in daily practice. The causes are gastroduodenal ulcers, vascular abnormalities, tumours, iatrogenic and Mallory Weiss syndrome. Several hemostasis endoscopic methods are being used to treat the hemorrhage.
Objectives: To find out causes and hemostasis procedures in NVUGIH patient at digestive endoscopy department Cipto Mangunkusumo hospital Jakarta.
Materials and Methods: This is a descriptive cross sectional study from medical record data that include 79 NVUGIH patient underwent endoscopic hemostasis procedure at digestive endoscopy department from January 2020 to June 2024.
Results: Endoscopic hemostasis were most performed in male patient (77,2%) at aged range of 41 to 60 years (39,2%). Melena is the most common indication (64,5%). The hemorrhage were caused by peptic ulcer disease (37,9%), gastroduodenal tumours (29,1%), iatrogenic (29,1%), arteriovenous malformation (2,5%) and foreign body (1,26%). Adrenaline injection (AI) combine with hypertonic dextrose spray (HDS) is the most common hemostasis procedure (34,1%). The others are HDS (20,2%), hemoclip (12,6%), Argon plasma coagulation (APC) with HDS (5,06%), hemoclip with AI (3,79%), hemoclip with HDS (3,79%), APC with AI and HDS (3,79%), APC (2,5%), APC with AI (2,5%), hemoclip with APC and AI (1,26%), hemoclip with APC (1,26%), hemostatic forcep with HDS (1,26%) and hemostatic forcep with APC, AI and HDS (1,26%).
Conclusion: Peptic ulcer disease and combination between adrenaline injection with hypertonic dextrose spray is the most common cause and hemostasis procedure on NVUGIH patient at digestive endoscopy department Cipto Mangunkusumo hospital Jakarta.
PP-01-148
Transnasal versus conventional gastroscopy: comparison of tolerability and diagnostic outcome
Kimchhay Ro1, Chakravuth Oung2 and Sereyvathana Chhut2
1Calmette Hospital, Phnom Penh, Cambodia;2University of health sciences, Cambodia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The main objective of this study was to assess tolerability and diagnostic outcome of transnasal esophagogastroduodenoscopy (T-EGD) in comparison with conventional esophagogastroduodenoscopy (C-EGD).
Materials and methods: This was a retrospective, descriptive, analytical, monocenter study and was conducted from 2010 to 2017 which included 3,637 patients over the age of 18 years old who underwent endoscopy without sedation using 5.5 mm and 9.2 mm endoscope. Patient data were noted regarding tolerability, acceptance and endoscopy performance using visual analogue scale (VAS) and diagnostic outcome.
Result: In 3637 patients underwent unsedated gastroscopy (T-EGD 1738, C-EGD 1899), benign and malignant findings are comparable in both group. VAS scores for patient tolerability were significantly better in the T-EGD group (7.09 vs 6.07, P < 0.001). T-EGD was more comfortable and better tolerated than previous endoscopy in 87% of C-EGD experienced patient (34 out of 39). 89.8% of T-EGD group preferred the same procedure for their future endoscopy, compare to 70.9% in C-EGD group.
Conclusion: Transnasal endoscopy is superior to C-EGD in terms of comfort and patient acceptance while maintaining an efficient diagnosis and acceptable performance. T-EGD is suitable as an alternative endoscopy exam for patients who are at high risk of sedation-related complications and can't tolerate conventional gastroscopy.
PP-01-149
The Evolution of Double Pylorus Associated With NSAIDs-Induced Gastric Ulcer
Rasco Sandy Sihombing1, Taolin Agustinus1 and Ari Fahrial Syam2
1RSUD Mgr Gabriel Manek, SVD, Atambua, Atambua, Indonesia;2Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia / Cipto Mangunkusumo General National Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Double pylorus (DP) is an uncommon condition when two communicating channels between the gastric antrum and duodenal bulb exists. According to the etiology, DP can be categorized as acquired and congenital. Acquired DP is usually preceded by gastric ulcer.
Case Report: A 77-year old male patient with the history of frequent using of non steroidal anti-inflammatory drugs (NSAIDs) was admitted because of hematemesis melena. The esofagogastroduodenoscopy (EGD) showed the two openings separated by bridging tissue at the end of the antrum. Both openings were led the scope to the duodenal bulb. There was also an ulcer at small curvature side of the antrum. Nine months later, EGD showed one large opening connecting the antrum and duodenal bulb suggested as fusion between the two channels.
Discussion: The prevalence of DP is reported about 0,001-0,4%. The acquired DP is possibly formed by the advanced erosion between gastric and duodenal ulcer. Another mechanism is the gastric scarring which attracts the lesser curvature and later perforated into the duodenal bulb. The congenital type of DP is less often and usually occurs in young patients with other gastrointestinal congenital abnormalities. The single large opening in the third endoscopy is the fusion between the two channels caused by septal destruction that may be related to fistulous tract ulceration or mechanical damage by food bolus. The treatment of acquired DP is just focused on factors that influence the gastric ulcer like stopping the NSAIDs and eradicating the H. pylori. Surgery is extremely rare to perform.
PP-01-150
Massive obscure lower gastrointestinal bleeding from predominant ectopic pancreatic tissue in a Meckel’s diverticulum
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka;3Department of Pathology, University of Colombo, Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Obscure gastrointestinal bleeding(OGIB) represents about 5% of all gastrointestinal haemorrhages which is characterized by continuous or recurrent bleeding from an undetermined source after an initial negative bidirectional endoscopy. Meckel's diverticulum(MD) is a rare but recognized cause of OGIB which usually contain ectopic gastric mucosa. Here we present a patient with MD contained predominant ectopic pancreatic tissue resulted in massive OGIB.
Case Description: A 35-year-old female with recurrent rectal bleeding over 7 years and severe anemia underwent multiple inconclusive investigations. Previous episodes were resolved with blood transfusions. On admission, she was pale, tachycardic, and hypotensive. Initial tests, including a CT angiogram, bidirectional gastrointestinal endoscopies and a small bowel capsule endoscopy, did not provide a clear cause. She experienced massive bleeding, requiring emergency laparotomy which revealed a MD with thickened mucosa. An improvised intraoperative endoscopy via appendix confirmed no colon bleeding, leading to resection of the diverticulum. Her post-operative period was uneventful with stable haemoglobin levels. Histology showed predominant ectopic pancreatic mucosa with a focus of gastric tissue in the resected MD.
Discussion: MD and its complications, especially gastrointestinal bleeding, are more common in children than in adults. This case discusses the rarity of histological presentation of MD with predominant pancreatic tissue leading to massive OGIB. It also discusses the importance of intraoperative decision-making and intra operative enteroscopy in cases of OGIB when other tests are negative before surgical resection of symptomatic MD.
PP-01-151
A UK hospital experience on feasibility of transnasal endoscopy (TNE) service in outpatients setting
Cheh Kuan Tai
Royal Free NHS Trust, London, London, United Kingdom
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
During the height of the COVID-19 pandemic in 2020, elective endoscopy services within the UK National Health Service came to a standstill for 4 months. With subsequent waves, the redeployment of endoscopy staff further compounded the endoscopy backlog, with surveillance procedures being impacted the most.
In the UK, endoscopy services are delivered in dedicated endoscopy units. Gastroscopies are generally performed with local anaesthetic with or without sedation. Each endoscopic list requires 2 assistants-one trained nurse and one health care assistant.
In 2022, our gastric ulcer rescope audit showed that 70% of patients did not receive their repeat procedure within 12 weeks. We developed a transnasal endoscopy (TNE) service to increase capacity. TNE cause less haemodynamic instability. It is generally better tolerated and obviate the need for sedation. The nursing provision could also be reduced to 1 trained nurse.
We began a pilot TNE service in October 2023 in the outpatient clinic setting. In the 9 months between October and June 2023, we have delivered 31 TNE lists and 172 cases. The numbers of cases completed per list was initially low at 4 to allow for training of staff. There has not been any complications encountered thus far. 4% of patients did not tolerated the procedure unsedated and had to be rebooked.
This service has allowed us to clear the surveillance backlog for gastric ulcer and oesophagitis rescopes and we are currently performing prospective diagnostic requests. This pilot demonstrates the feasibility of a TNE service based in outpatients.
PP-01-152
Sloughing Esophagitis In A 84-Year-Old Patient: A Case Report
Pham Phuong Thu Tran and Ngoc Luu Phuong Tran
Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Viet Nam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Sloughing esophagitis is characterized by sloughing of large vertical fragments on the superficial esophageal mucosa without inflammation. Due to the absence of specific features indicative of esophageal disorders, this disease has a very low incidence and is often unexpectedly diagnosed by esophagogastroduodenoscopy (EGD). Risk factors involve medications, toxic chemicals, or immune system disorders, but the primary cause remains unknown. In this case report, we describe the patient’s characteristics and compare them with the previous case reports to find out appropriate diagnosis and treatment approach for subsequent cases.
Case description: A 84-years-old man was admitted to hospital with chief complain of two-day melena and a notable symptom was hiccups for two weeks leading to malabsorption. His medical history included hypertension and atopic dermatitis, and unremarkable signs at diagnosis. Therefore, urgent EGD was performed, revealing a peptic ulcer with the Forrest type III and suspected sloughing esophagitis. Further examination reminded him that he had been experiencing heartburn, retrosternal pain, dysphasia with solid and liquid food for the past month, but these symptoms had been not noteworthy. Finally, he was partially recovered by intravenous proton pump inhibitor (PPI) after 10-day treatment.
Discussion: Sloughing esophagitis occurring in the middle – distal esophagus is a benign and self-limited illness. In cases of severe erosion reflux disease or nonvariceal upper gastrointestinal bleeding, PPI has shown efficacy. However, refractory esophagitis may require additional treatment with intravenous glucocorticoids. Given the nonspecific symptoms, patients with sloughing esophagitis should be re-evaluated by repeat EGD to monitor recovery and complications.
PP-01-153
Long-term PPI users should avoid alcohol consumption and receive endoscopy follow up regularly
Yu-Ching Tsai1,2, Hsiao-Bai YANG3,5, Ming-Tsung HSIEH2, Chun-Te LEE2, LO Yi-Ning1,2, Hsiu-Chi CHENG2,4,6 and Bor-Shyang SHEU2,4
1Tainan hospital ministry of health and welfare, Tainan, Taiwan;2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;3Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;4Institute of Clinical Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan;5Department of Pathology, Ton Yen General Hospital, Hsin-Chu, Taiwan;6Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to identify risk factors associated with the presence/progression of pre-cancerous lesions such as advanced gastric atrophy/intestinal metaplasia (OLGA/OLGIM stage III/IV) or corpus-predominant gastritis index (CGI) and whether endoscopic features of precancerous lesions were associated with pathological diagnosis among long-term PPI users.
Materials and methods: 73 long-term PPI users were enrolled to receive gastroscopy and topographic biopsies and assess the presence of OLGA/OLGIM stage III/IV and CGI. With comparisons with their previous endoscopic studies before PPI use, changes of OLGA/OLGIM and CGI were compared to risk factors and NBI endoscopy images.
Results: We found consistencies between the previous and current high-risk IM features (OLGIM III/IV+corpus IM) (P<0.05). Alcohol consumption was associated with the presence of CGI (P=0.002). Under NBI endoscopy, the Kimura-Takemoto classification, EGGIM, and corpus mucosa with groove pattern/dilated sub-epithelial capillaries were associated with OLGA stage III/IV (P=0.048), OLGIM stage III/IV (P<0.001), high corpus inflammation (P<0.001), and CGI (P=0.05). As compared with previous biopsies, the progression rates of OLGA III/IV, OLGIM III/IV/corpus IM, and CGI were 20.3%, 3.4% and 10.5%, respectively. The progression/regression of IM and CGI based on pathology was moderately associated with the progression/regression based on endoscopic images, including EGGIM (Pearson’s R=0.609, P<0.001) and corpus inflammation (Pearson’s R=0.396, P=0.003) after long-term PPI use. Alcohol drinking was associated with the progression of precancerous lesions (P=0.023).
Conclusion: Alcohol was a risk factor of CGI and the progression of precancerous lesions among long-term PPI users. Endoscopic features after long-term PPI use were correlated with pathological diagnosis.
PP-01-154
Gastric epithelial neoplasm of fundic-gland mucosa lineage
Hiroya Ueyama and Takashi Yao and Yoichi Akazawa and Takuo Hayashi and Tsutomu Taeda and Kumiko Ueda and Mariko Hojo and Akihito Nagahara
Department Of Gastroenterology, Juntendo University School Of Medicine, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: We previously proposed a histopathological classification of gastric epithelial neoplasm of fundic-gland mucosa lineage (GEN-FGML) [1,2]. However, the clinicopathological features in a large number of cases have not been well investigated. The aim of this study is to clarify the clinicopathological features of GEN-FGML according to the histopathological classification.
Materials and Methods: We performed a retrospective study to investigate the clinicopathological and endoscopic features of GEN-FGML. GEN-FGML was classified into 3 major types, oxyntic gland adenoma (OGA), gastric adenocarcinoma of fundic-gland type (GA-FG), and gastric adenocarcinoma of fundic-gland mucosa type (GA-FGM).
Results: A total of 150 GEN-FGML lesions in 113 patients were retrospectively collected between July 2008 and March 2024. The average of tumor size was OGA 3.8mm, GA-FG 7.9mm, and GA-FGM 9.5mm. The depth of submucosal invasion (μm, GA-FG 275.7, GA-FGM 555.9, p<0.05) were significantly greater and the rates of lymphatic invasion (GA-FG 1/70, GA-FGM 3/26, p<0.05) were significantly higher in GA-FGM than in GA-FG. The rate of non-curative endoscopic resection was significantly higher in GA-FGM than in GA-FG (GA-FG 8/69, GA-FGM 7/15, p<0.01). OGA and GA-FG demonstrated low-grade epithelial neoplasm, and GA-FGM should be categorized as an aggressive variant of GEN-FGML that demonstrated high-grade epithelial neoplasm as reported previously.
Conclusion: This classification is useful to estimate its malignant potential and establish a standard therapeutic approach for GEN-FGML.
References:
[1] Ueyama, H et al. J Gastroenterol.2021. 56(9): 814-828.
[2] Ueyama, H et al. Am J Surg Pathol.2010. 34(5): 609-19.
PP-01-155
A Novel Suturing Technique for the Endoscopic Full-Thickness Resection Defects Using a Long Transparent Cap
Saif Ullah, Nan Dai and Xinguang Cao
The First Affiliated Hospital Of Zhengzhou University, Zhengzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To evaluate the safety and efficacy of a novel long transparent cap suturing technique for the closure of large endoscopic full-thickness resection (EFTR) defects.
Methods: A total of 62 patients who underwent EFTR for their large gastric submucosal tumors at the First Affiliated Hospital of Zhengzhou University between January 2017 and March 2023 were included in this study. All the EFTR defects was closed using the long transparent cap suturing technique (Graphical abstract). The procedure success rate, tumor size, defect closure time, length of postoperative hospital stay, complications and recurrence rate were recorded and analyzed.
Results: The procedure technical success rate was 100%. The median tumor size was 1.5 cm (range: 0.6-6.5 cm), and the median number of metal clips used was 7 (range: 4-14). The median EFTR procedure time was 53.5 minutes (range: 22-136 minutes), and the median suturing time was 14 minutes (range: 3-45 minutes). The median length of postoperative hospital stay was 3.5 days (range: 3-6 days). Postoperative complications including bleeding occurred in 1 patient, abdominal pain in 2 patients, and fever observed in three patients. During the median 29 months (range: 7-79 months) of follow up, no residual lesions, tumor recurrence, or metastasis were detected, and no formation of digestive tract fistulas or sinuses was observed.
Conclusion: The long transparent cap suturing technique is a simple, safe, and effective method for large defects closure after EFTR. Its advantages includes, affordability, simplicity, and widespread availability, making it suitable for widespread clinical application.
PP-01-156
The smart approach to surgical treatment for gastric and duodenal gists based on preoperative eus-typing
Seda Dzhantukhanova and Yury Starkov and Ayubkhan Vagapov and Rodion Zamolodchikov and Amina Badakhova
Vishnevsky National Medical Research Center for Surgery, Moscow, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To develop and demonstrate different surgical techniques of laparoscopic or endoscopic resection for GISTs based on classification of EUS-typing for optimal choice of treatment
Materials and Methods: The EUS-classification of GISTs was created based on the analysis of treatment of 80 patients with gastric and duodenal GISTs. The principles of classification included the following criteria: location of tumor base in relation to GI layer Size of the tumor base (“growing point”) Type of growth in relation to GI lumen The EUS-typing includes Type I, Type II, Type III (a, b, c, d) tumors (table)
Results: Patients with gastric and duodenal GISTs underwent laparoscopic resection – 62, endoscopic intraluminal resections – 18 patients (tunneling resection – 7, endoscopic submucosal dissection or endoscopic enucleation of tumor after resection of covering mucosa – 11) Median operation time was 150 min. Recovery was uneventful and median post-op hospital stay was 5 ± 2,4 (2-8) days. The pathology showed R0 resection in all cases. Histology and immunohistochemistry confirmed GIST. Four patients received adjuvant target therapy and were prescribed Gleevec for 1-2 years. The mean follow-up period was 42 months (range 3-74 months) with no local or distant recurrence or stenosis at the site of surgery
Conclusion: The classification of GISTs based on EUS-typing allows to select the optimal approach individually for each patient to perform surgery more accurate and less invasive
PP-01-157
EXPLORE AI-BO Study: Exploring Endoscopists’ Knowledge and Perception Towards Artificial-Intelligence in Surveillance for Barrett’s Oesophagus
Nicholas Wan1,2, Jin Tan1,2, Celine Chan1, Asif Chinnaratha1,2 and Rajvinder Singh1,2
1Gastroenterology Department, Lyell McEwin Hospital, Elizabeth Vale, Australia;2Adelaide Medical School, University of Adelaide, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Artificial Intelligence (AI) has been shown to assist endoscopists in detecting early neoplasia in Barrett’s Oesophagus (BO). We aim to explore endoscopists’ knowledge and perception towards AI in BO, providing insights into factors impacting its clinical implementation.
Methods: A questionnaire consisting of six domains was disseminated to 140 endoscopists across South Australia.
Results: Twenty participants completed the questionnaire. 1) Knowledge and Perception Towards AI in Endoscopy: 15% reported having adequate knowledge of AI-application in BO, compared to colorectal-polyp-detection (60%). Majority (85%) were willing to use AI for BO-surveillance. 2) Knowledge and Perception Towards AI in BO-Surveillance: White-Light-Imaging (60%) and Narrow-Band-Imaging (55%) were modalities where respondents had some knowledge of AI in BO-surveillance. Participants perceived that AI will improve the detection rates of low-grade-dysplasia (90%), high-grade-dysplasia (95%) and early-oesophageal-cancer (85%). 3) Impact on Patients: Half anticipated that AI would reduce the frequency of endoscopic surveillance. 4) Impact on Endoscopists: 75% indicated that AI could extend procedural duration. While some suggested an increase in fatigue (25%), others believed it decreases fatigue (50%). 5) Impact on Clinical Practice: All respondents felt that AI would enhance their accuracy in dysplasia detection. 70% opted to perform oesophageal biopsies even if AI had >95%sensitivity for detecting early-Barrett’s-dysplasia. 6) Medico-legal Concerns: Without regulation, only 50% were open to using AI. Accountability of missed-lesions was felt to be the responsibility of endoscopists (70%) vs AI (5%).
Conclusion: Endoscopists showed positive attitudes towards integrating AI in BO-surveillance. Potential concerns include liability, procedural duration, and lack of regulation.
PP-01-158
Effect of GLP-1RA on residual gastric content during esophagogastroduodenoscopy:Systematic Review and Meta-Analysis of Cohort studies
Pamela Bianca Yap, Mabel Angela Sarita and Sarah Jean Bellido
St.lukes medical center, Quezon city, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Glucagon-Like Peptide–1 Receptor Agonists(GLP-1 RA) have been used for the treatment of diabetes and management of obesity. Recent data shows increased incidence of endoscopically visible residual gastric contents (RGC) even with adequate fasting, predisposing sedated patients to adverse events. The American Society of Anaesthesiologists released a consensus guideline, holding GLP-1 RAs in the perioperative period though evidences were limited to several case reports. The objective of this study is to assess the effect of GLP-1 RA on RGC during upper endoscopy and risk for bronchopulmonary aspiration.
Materials and Methods: A comprehensive search through different online data base was done which included studies determining the association of GLP-1 RA on RGC and bronchopulmonary risk during upper endoscopy. The quality of included studies was assessed using the Newcastle-Ottawa Scale(NOS) and GRADE Pro. Data was analyzed using Revman5.4. The primary outcome was presence of RGC and the effect measure used was odds ratio with confidence interval of 95%. Secondary outcome was bronchopulmonary risk
Results: The search yielded 209 articles and 4 studies were included in this review. The studies included were assessed to have low risk of bias. Pooled results from the 4 studies showed increased RGC with GLP-1 RA use (OR 6.36, CI 5.20,7.78, p-value <0.00001). Bronchopulmonary risk increased with GLP RA use(OR 6.57, CI 1.35, 31.98, p-value <0.02). The studies, however, had very low to low certainty of evidence due to issues on study design and inconsistency.
Conclusion: GLP-1 RA was associated with increased RGC in patients undergoing upper endoscopy.
PP-01-159
High-magnification NBI is an accurate tool for diagnosis of gastric neoplasia in a western population
Edward Young1,2, Nicholas Wan1, Andrew Ruszkiewicz1,3 and Rajvinder Singh1,2
1Lyell McEwin Hospital, Northern Adelaide Local Health Network, Adelaide, Australia;2Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, Australia;3SA Pathology, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: In high-incidence eastern countries, nationwide gastric cancer screening programs have reduced the incidence of advanced gastric cancer by facilitating detection at an early stage amenable to endoscopic resection. In these countries, high-magnification narrow band imaging (M-NBI) has been established as an effective advanced mucosal imaging technique for gastric lesions. We aimed to assess the accuracy of this technique in a western population where data is limited.
Materials and Methods: This is an analysis of a prospectively collected database of gastric lesions that were assessed and either biopsied or resected by an interventional endoscopist at a single Australian Hospital between 2009 and 2023. All lesions were assessed using M-NBI and a histological prediction made at the time of reporting. This was then correlated with the final histology.
Results: A total of 232 lesions in 183 patients were included in the final analysis, including 35 adenomas, 29 early gastric cancers, 6 invasive adenocarcinomas, 137 non-neoplastic lesions and 25 ‘other’ lesions. For differentiating neoplastic versus non-neoplastic lesions, M-NBI had a sensitivity of 97.9% (CI 92.6-99.7%) and specificity of 97.1% (CI 92.7-99.2%). M-NBI also had high specificity (97.1%, CI 92.7-99.2%) in identifying lesions suitable for endoscopic resection, with a PPV of 96.9% (CI 89.2-99.6%). The observed agreement between the M-NBI predicted histology and the final pathological diagnosis was 91.81% with a derived kappa statistic of 0.865, indicating excellent agreement.
Conclusion: M-NBI can be used with a high degree of accuracy in western populations, comparable to the accuracy demonstrated by experts in eastern countries.
PP-01-160
Risk Factors and Impact of Missed Adenomas in Colonoscopic Polypectomy with Different Endoscopic Systems
Chi Hyeon Choi and Jong Yoon Lee
Dong-a University Hospital, Busan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to identify missed adenomas not detected in previous screenings or colonoscopies during polypectomy and to determine if the endoscopic system impacts the miss rate.
Materials and Methods: This retrospective study includes patients who underwent colonoscopic polypectomy at Dong-A University Hospital from March 2020 to February 2022. Patients were referred after polyps were detected during screening or surveillance colonoscopies at other facilities. Colonoscopic polypectomies were performed by an expert using either the 'latest system' or the 'older system.' Missed polyps or adenomas were defined as those not documented in previous electronic photographs or endoscopic reports but detected during polypectomy. Miss rates were calculated by dividing the number of missed polyps/adenomas/advanced neoplasia by the total number of each among all patients. Advanced neoplasia was defined as any adenoma ≥ 10 mm, with villous histology, high-grade dysplasia, invasive cancer, or any SSP ≥ 10 mm or with dysplasia.
Results: Out of 542 polyps, 186 were missed during initial screenings, resulting in a polyp miss rate of 25.55%. The miss rates for adenomas and advanced neoplasms were 27.34% and 14.69%, respectively. Univariate logistic regression analysis identified age, adenoma per colonoscopy, and endoscopic system as significant factors for adenoma detection. However, multivariate logistic regression analysis revealed that only the endoscopic system was statistically significant.
Conclusion: The use of an older endoscopic system is significantly associated with missed adenomas detected by colonoscopic polypectomy.
PP-01-161
Factors predicting mortality in End Stage Renal Disease patients presenting with upper GI bleeding
Raja Taha Yaseen Khan and Mahboob Jan and Abbas Ali Tasneem and Nasir Hassan Luck
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: This study aims to identify factors that predict in-hospital mortality in ESRD patients presenting with non-variceal UGIB.
Methods: A prospective cohort study was conducted at the Department of Hepato-gastroenterology, Sindh Institute of Urology and Transplant (SIUT), Karachi. The study included 209 ESRD patients on hemodialysis with non-variceal UGIB. Data collection encompassed demographic information, clinical presentations, laboratory findings, and endoscopic outcomes. Statistical analyses were performed using SPSS version 22.0, including univariate and multivariate analyses to identify independent predictors of mortality.
Results: Among the 209 patients, 121 (57.9%) were males. The most prevalent comorbidities were ischemic heart disease (37.8%), hypertension (35.4%), and diabetes (26.8%). Endoscopic findings showed that 95.2% had gastric ulcers. Key clinical presentations included tachycardia (49.8%) and hypotension (34%). The mortality rate was 44% (92 patients). Significant predictors of mortality identified in univariate analysis included male gender (p=0.002), diabetes (p<0.001), ischemic heart disease (p<0.001), hypotension (p<0.001), tachycardia (p<0.001), and specific endoscopic findings (gastric erosions, Forest Class Ia and IIa ulcers). Multivariate analysis revealed diabetes (p=0.01), hypotension (p=0.001), tachycardia (p<0.001), Forest Class Ia ulcers (p<0.001), increased INR (p=0.009), decreased platelet count (p=0.002), and administration of dual endoscopic therapies (p<0.001) as independent predictors of mortality.
Conclusion: ESRD patients with non-variceal UGIB have a high mortality rate. Significant predictors of mortality include diabetes, hypotension, tachycardia, specific endoscopic findings, increased INR, decreased platelet count, and the need for dual endoscopic therapy. Identifying these risk factors can guide clinical decision-making, improve management strategies, and potentially reduce mortality in this vulnerable population.
PP-01-162
Nurse-Driven Triage Assessment of Small Bowel Capsule Endoscopy Recordings
Tze Lee Yong, Anne Davarias, Josephine Khu, Calista Koh, Liwen Xue and Suzie Ji
Endoscopy, Chris O'Brien Lifehouse, Sydney, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Small-bowel video capsule endoscopy (SBVCE) offers a valuable diagnostic tool for small intestinal pathologies, particularly following inconclusive gastroscopy and colonoscopy. International gastroenterological societies endorse the utilization of appropriately trained and supervised nurse endoscopists (NEs) for SBVCE image interpretation and triage. While resistance to NEs substituting for physician endoscopists exists, studies demonstrate their competence in reviewing SBVCE images. Given the rising demand for SBVCE, this paper explores the potential for a novel non-physician reader role in Australia. Specifically, discussing the feasibility of endoscopy nurses performing SBVCE pre-reading and triage, aiming to streamline workflow, optimize resource allocation and improve patient care.
Methods: A comprehensive literature search was conducted using key terms: "endoscopy nurse", "trained", "pre-read", "interpret" and "capsule endoscopy". The search focused on English peer-reviewed articles published between 2017 and 2022, including systematic reviews and comparative studies that evaluated lesion detection accuracy by trained endoscopy nurses compared to gastroenterologists.
Results: Analysis of 18 studies supports nurse-led initial review of SBVCE images where nurses achieved high accuracy, demonstrating competence. There is also improved efficiency through cost reduction and saved physician time. The medical community embraced trained nurses performing SBVCE pre-reading, with artificial intelligence (AI) showing promise for further enhancement. This evidence supports implementing nurse pre-reading for SBVCE lesion classification while maintaining diagnostic quality.
Conclusion: This study supports the feasibility of appropriately trained and supervised endoscopy nurses performing SBVCE pre-reading and triage. Implementing this role could optimize physician time allocation, reduce healthcare costs, and improve patient access to care through shorter wait times.
PP-01-163
Linked color imaging versus white light imaging for the detection of gastric superficial neoplasia
Jae Gon Lee1, Sang Pyo Lee2 and In Kyung Yoo3
1Hallym University College of Medicine, Hwaseong, South Korea;2Hanyang University College of Medicine, Seoul, South Korea;3CHA University, Seongnam, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The early detection of gastric superficial neoplasia (GSN) by screening endoscopy is essential, but endoscopic detection and diagnosis are sometimes difficult. Linked color imaging (LCI) can help reduce the GSN miss rate and increase the tumor detection rate compared with white light imaging (WLI). We aimed to determine whether LCI is superior to WLI for detecting and diagnosing new GSNs by screening endoscopy.
Materials and Methods: This randomized, multi-center, prospective study was conducted on patients with suspected or diagnosed gastric adenoma or early gastric cancer. Endoscopic evaluations in the LCI and WLI groups were performed in LCI and WLI modes, respectively. After the first observation was completed in each group, additional observations were performed in a different mode. The GSN miss rate and tumor detection time and characteristics were evaluated.
Results: Forty-eight (52 tumors) of the 95 patients included underwent LCI (the LCI group), and the remaining 47 (52 tumors) underwent WLI (the WLI group). GSN miss rates in the LCI and WLI groups were 5.77% and 13.46%, respectively. Mean tumor detection time in the LCI group was significantly shorter than in the WLI group (p=0.049). Tumors not detected by first observation were significantly more likely to have a IIb morphology (p=0.014).
Conclusion: LCI may help reduce the GSN miss rate and enable faster detection. IIb tumors may be more difficult to detect endoscopically than other tumors.
PP-01-164
Loop-10 Technique for Mucosotomy Defect Closure After Esophageal Per-oral Endoscopic Myotomy (POEM)
Marc Julius Navarro1, Haruhiro Inoue2 and Yuto Shimamura2
1Institute of Digestive and Liver Diseases, St. Luke's Medical Center, Quezon City, Philippines;2Digestive Diseases Center, Showa University Koto Toyosu Hospital, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: In more challenging esophageal per-oral endoscopic myotomy situations, mucosotomy extension, laceration and defect widening may happen. It is more technically demanding to close a large-size mucosotomy defect solely by endoscopic clips. The objective of this study is to investigate the feasibility and efficacy of Loop-10 technique for mucosotomy defect closure after esophageal per-oral endoscopic myotomy.
Materials and Methods: Loop-10 technique were done in 30 patients for mucosotomy defect closure after esophageal per-oral endoscopic myotomy. After the execution of myotomy, the constructed first clip with loop-10 was advanced through the instrument channel of the single-channel therapeutic endoscope and was deployed in the most distal part of the mucosotomy defect. The assistant provided adequate traction and lift by pulling the support thread. Succeeding endoscopic clips were deployed to ride on the main loop, to achieve full closure of the defect. The primary outcome measure was the complete closure rate. The secondary outcome measures were sustained closure rate, defect size, total number of clips deployed, and mucosotomy defect closure time.
Results: Complete closure and sustained closure rate of 100% were achieved. The mean mucosotomy defect length was 2.28cm (SD +/- 0.67, range 1.50-4.00cm). The mean total number of clips deployed to achieve complete closure were 3.93 clips (SD +/- 0.83, range 3-6 clips). The mean mucosotomy defect closure time was 4.80 minutes.
Conclusion: Loop-10 technique presented to be a feasible and effective method in achieving complete closure and sustained closure of mucosotomy defect after esophageal per-oral endoscopic myotomy.
PP-01-165
Comparison of Magnifying Endoscopy with NBI and EUS for Predicting Tumor Invasion Depth in EGC
Jun Chul Park1, Soo In Choi2 and Da Hyun Jung1
1Yonsei University College Of Medicine, Seoul, South Korea;2Inje University Sanggye Paik Hospital, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: In early gastric cancer (EGC), magnifying endoscopy with narrow-band imaging (ME-NBI) can predict the invasion depth by yielding clear images of the surface and microvascular pattern of tumor. We aimed to compare the diagnostic accuracy of ME-NBI in T-staging of EGC compared with conventional endoscopic ultrasonography (EUS). Material and Methods: In this prospective, randomized, non-inferiority trial, patients with EGC were randomly allocated in a 1:1 ratio to two parallel groups: ME-NBI (n=81) or EUS (n=84). The invasion depth in ME-NBI was determined based on microsurface patterns, microvascular patterns, and multicaliber vessels. Diagnostic performance for the invasion depth in each group was compared using the final histopathological diagnosis. A non-inferiority margin of -10% for the invasion depth was assumed.
Results: The diagnostic accuracy, specificity, positive predictive value, and negative predictive value for T1sm showed no statistically significant differences between ME-NBI and EUS (66.7% versus 53.6%, p=0.0861; 64.5% versus 58.7%, p=0.5060; 38.9% versus 23.5%, p=0.1665; and 88.9% versus 74.0%, p=0.0645); however, the sensitivity was significantly higher in ME-NBI (73.7% versus 38.1%, p=0.0239). In the non-inferiority test, ME-NBI revealed non-inferiority to EUS in predicting invasion depth. Additionally, the accuracy of ME-NBI was significantly higher than that of EUS in tumors > 20 mm, depressed tumors, and T1sm1 tumors (73.2% versus 48.7%, p=0.038; 70.7% versus 46.3%, p=0.043; 71.4% versus 12.5%, p=0.041).
Conclusions: The diagnostic capability of ME-NBI was comparable to that of EUS. ME-NBI could be a useful alternative modality for discriminating submucosal invasion of EGC, particularly in large, depressed or T1sm1 tumors.
PP-01-166
Erythromycin vs. Metoclopramide To Improve Endoscopy Visualization In Upper Gastrointestinal Bleeding: A Network Meta-Analysis
Adam Prabata1, Diski Saisa2, Isya Abiyyu Mumtaz2 and Saskia Aziza Nursyirwan3
1Department Of Internal Medicine, Faculty Of Medicine, Universitas Indonesia, Jakarta, Indonesia;2Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;3Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The aim of this study is to compare the efficacy of erythromycin and metoclopramide for endoscopic visualization improvement.
Materials and Methods: We searched online databases like PubMed, Scopus, and Cochrane Library, till August 20 for RCTs that compared erythromycin vs. metoclopramide with gastric lavage or placebo. We used the keywords “erythromycin”, “metoclopramide”, “endoscopy visualization”, and “upper gastrointestinal bleeding”. The risk of bias was evaluated with Cochrane Risk of Bias 2 (RoB 2). Frequentist network meta-analysis was performed to pooled the odds ratio (OR) or standardized mean difference (SMD) with 95% confidence intervals (CI). Outcomes were endoscopy visualization, repeat endoscopy, and duration.
Results: A total 13 of RCTs that evaluated 1361 patients were included in this systematic review. Treatment consists of erythromycin, metoclopramide, control (gastric lavage or placebo), and its combination. Compared to placebo, combination of erythromycin with gastric lavage has better efficacy for satisfactory visualization (OR 9.30; 95% CI: 2.40, 36.10; I²: 38.7%) and visual endoscopy score (SMD 1.18; 95% CI: 0.44, 1.92; I²: 72.3%, but not significant for endoscopy duration (SMD 0.77; 95% CI: -0.82, 2.36; I²: 94.3%) and second look endoscopy (OR 0.41; 95% CI: 0.15, 1.17; I²: 0%).
Conclusion: In this review, we concluded that administration of combination of erythromycin with gastric lavage could help improve endoscopy visualization in upper GI bleeding. However, high quality RCT are required to verify these findings.
Keywords: erythromycin, metoclopramide, endoscopy visualization, upper GI bleeding, network meta-analysis
PP-01-167
Nasopharyngeal examination during transoral upper gastrointestinal endoscopy: Is it feasible?
Rasyiqatul Raminey and Rafidah IDRIS and Sumitro Kosasih and Norwani Dewi Basir and Vui Heng Chong
Ripas Hospital Brunei, Bandar Seri Begawan, Brunei Darussalam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Transoral upper gastrointestinal endoscopy (UGIE) is a common investigation for upper gastrointestinal symptoms. The nasopharynx (NP) is not part of the examination but can be examined in a retroflexed position. This study assesses the feasibility of NP examinations during routine transoral UGIE.
Materials and methods: All patients who had transoral UGIE by an experience endoscopist over a period was reviewed and analysed. A total 144 patients (mean age 53.2 ± 14.8 years, male 50.7%, overweight/obese 52.8%) were included in the study. Procedures were performed without sedation (41.7%), conscious sedation (52.8%) and general anesthesia (5.6%).
Results: 75.7% (n=109) had adequate NP examination and visualizations of the structures were as follow: adenoid pad (100%), nasal septum (99.1%), turbinate (superior 98.2%, middle 94.5%), eustachian tubes (left 90.8%, right 84.4%), torus tubarius (right 97.2%, left 96.3%) and the fossa of Rosenmuller (right 88.1%, left 90.8%). Of the fossa of Rosenmuller, only 40.4% (right) and 47.7% (left) had adequate visualization of the bases. Reasons for failure for NP examinations included gag reflex, bleeding, including epistaxis from the adenoid pad from trauma during retroflexion, coughing and tongue movement. There was no difference between the genders (p=0.380), weight status (normal/underweight vs. overweight/obese, p=0.865), caliber of scope (9mm vs. 11mm, p=0.970) and sedation status (p=0.350 for trend) in successful NP examinations.
Conclusions: Our study showed that NP examination is feasible during transoral UGIE with good visualization of the anatomies. There were no factors predictive of failure to examine the NP.
PP-01-168
“Segment Anything” with MedSAM: Delineating Barrett’s Dysplasia Using Artificial Intelligence
Jin Lin Tan1,2, Ka Hing Chan3, Dileepa Pitawela3, Mohamed Asif Chinnaratha1,2, Hsiang-Ting Chen3 and Rajvinder Singh1,2
1Department of Gastroenterology, Lyell McEwin Hospital, Elizabeth Vale, Australia;2Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, Australia;3Australian Institute for Machine Learning, The University of Adelaide, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to evaluate the performance of MedSAM, a cutting-edge segmentation model trained on 1.5 million cancer images, on a dataset of Barrett’s dysplasia endoscopic images obtained using Narrow-band Imaging (NBI). Accurate segmentation is useful for highlighting subtle contour irregularities in lesions, which is applicable in computer-aided diagnostic endoscopies and speeds up the annotation of large endoscopic datasets.
Material and Methods: A total of 248 images from 36 patients with Barrett’s dysplasia were annotated and verified against histology reports. The dataset was divided into 196 training images from 29 patients and 52 testing images from 7 patients. A model using YOLO v8 was trained to localize dysplasia areas with bounding boxes, which MedSAM used for segmentation. The performance was evaluated using the Intersection over Union (IoU) for localisation and the Dice coefficient for segmentation accuracy.
Results: The model achieved an average IoU of 71.1% for Barrett’s dysplasia localisation, indicating a significant overlap between predicted and ground truth bounding boxes. For dysplasia segmentation, an average Dice coefficient score of 71.7% was attained, demonstrating good precision.
Conclusion: This study demonstrates the potential of using MedSAM to accelerate and scale the annotation of large endoscopic image and video datasets, as well as its applicability to real-time segmentation in endoscopy in the future. Accurate segmentation could also help endoscopists delineate margins better prior to performing endoscopic resection.
PP-01-169
Optimal Management Options for Esophageal Gastrointestinal Stromal Tumors (E-GIST)
Shanshan Zhu, Saif Ullah and Xin-Guang Cao
The First Affiliated Hospital Of Zhengzhou University, Zhengzhou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To explore the different treatment modalities for esophageal gastrointestinal stromal tumors (E-GIST) and their respective applicability and clinical outcomes.
Methods: This is a retrospective study in which consecutive patients diagnosed with E-GIST at our hospital from January 2017 to August 2023 were included. The clinical characteristics of all the patients as well as long-term quality of life were recorded and analyzed.
Results: A total of 23 (12 males, 11 females) E-GIST patients with a mean age of 56.7 ± 12.0 years were included in this study. Common symptoms, including upper abdominal pain, acid reflux, and heartburn, accounted for over 60% of cases. Fifteen patients underwent endoscopic resection, five patients underwent surgical resection, two patients underwent surgical resection after receiving preoperative imatinib therapy, and one patient received conservative management.
Conclusion: Different treatment strategies may be applied to the patients with E-GIST depending on the their clinical features. Our study provides insights into precise treatment for different patients. However, due to the rarity of the disease, it is challenging to collect a large sample size from a single center, necessitating more multicenter prospective large-scale studies.
PP-01-170
Gastric Volvulus: A Great Masquerade
Ankit Vats
ARMY HOSPITAL R&R, New Delhi, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Dr Ankit Vats1, Col Priyank Dhiman2
1.Senior Resident
2.Associate Professor
Dept of Gastroenterology and Transplant Hepatology, Army Hospital (R&R), Delhi Cantt-110010
Gastric Volvulus: A Great Masquerade
84 year old, male patient, known case of hypertension. presented with multiple episodes of non-bilious vomiting and painful distension of abdomen to emergency department in March 2024.
On clinical examination, he had respiratory rate 28 / min , spO2 > 95 % on room air, pulse rate of 110/min regular and blood pressure of 92/60 mm of Hg. Abdominal examination revealed distension in the epigastrium and umbilical region with guarding. Bowel sounds were reduced. All the hernia sites were normal and on digital rectal examination yellow stools were present in the Rectum. A clinical diagnosis of gastric outlet obstruction was made. X Ray abdomen and chest PA view showed elevated left dome of diaphragm. UGI Endoscopy was done showing a large hiatus hernia with LA Grade D esophagitis, grossly dilated stomach with significant food residue, a swirl of mucosa was seen in the stomach extending distally, antrum could not be located. The stomach appeared to be twisted along its longitudinal axis.
PP-01-171
Endoscopists’ knowledge, perceptions and attitudes towards the use of Artificial Intelligence in endoscopy
Nicholas Wan1,2, Celine Chan1, Jin Lin Tan1,2, Asif Chinnaratha1,2 and Rajvinder Singh1,2
1Gastroenterology Department, Lyell McEwin Hospital, Elizabeth Vale, Australia;2Adelaide Medical School, University of Adelaide, Adelaide, Australia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Artificial Intelligence (AI) is evolving in endoscopy. The perceptions of endoscopists towards AI remain poorly understood. We aim to summarize the perspectives of endoscopists on AI.
Methods: Online databases were searched to identify questionnaires conducted on endoscopists. Qualitative synthesis of included studies was conducted by categorizing into five domains: 1) Attitudes towards AI, 2) Impacts on endoscopy, 3) Impacts on endoscopists, 4) Impacts on patients, 5) Barriers towards AI implementation.
Results: Ten studies were included in this systematic review comprising of 1587 endoscopists across Europe (32.6%), North America (42.8%), and Asia (24.6%). Domain-1: Most (69–100%) had basic knowledge of AI. Majority (79.5–87.5%) expressed interest and optimism. Domain-2: Most (62.5–97%) supported the notion that AI would positively impact endoscopic performance and quality. Domain-3: There were mixed perceptions regarding operator-dependence (6.2–62.8% agreement), and whether AI would prolong procedural time (21–81.3% agreement). Most (71–100%) disagreed that AI would replace them. Domain-4: Majority (81.3%) felt that AI would increase the quality of patient-care. Only a minority (6.2–24%) felt that AI could improve the patient-physician relationship. Domain-5: Most (75.2–91%) identified costs as potential barriers to AI implementation. Opinions on ethics and the lack of regulation varied (12.5–100% and 35–88%, respectively), with majority feeling that clear guidelines and regulations was required.
PP-01-172
The overlooked connection between the inadequate water intake and dyspeptic symptoms among senior citizens.
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Dyspepsia characterized by discomfort in upper abdomen. One of the potential risk factors causing dyspepsia is dehydration.
Study Aim and objective: The aim of study is to investigate the water intake habit among the different age groups population and its association with dyspeptic symptoms by assessing the relationship of adequate water intake with dyspepsia according to SF-LEEDS questionnaire.
Patients and Methods: Cross Sectional, prospective study was conducted, in 2021-22, at the Department of Gastroenterology, Liaquat National Hospital, Karachi. Total 450 patients were enrolled for study after informed consent. Dyspepsia diagnosed by SF-LEEDS questionnaire with 32 max score. It was categorized into mild, moderate and severe with score of 10, 11-20, 21 respectively.
Results: Total 450 patients were enrolled in study with median age of 43 (IQR= 30-55) years. And (58.7%) were males. Patients presented with mild (n=19, 4.2%), moderate (n=95, 21.1%) and severe dyspepsia (n=336, 74.7%) with median Leed’s score of 20 (IQR= 17-22). Sign of dehydration including dry tongue (n=175, 38.9%), tachycardia (n=13, 2.9%) and reduce skin turger (n=3, 0.7%) were present among studied patients. Median daily water intake, recommended water intake, percent of having recommended water and SFLD score was 6 (5-7) glasses, 8.8 (7.5-10.16) glasses, 68.87% (54.76-84.18) and 20 (17-22) respectively. Our study showed that overall daily water intake habit was around 1-2 liters i.e., 50%-80% of the recommended daily water intake.
PP-01-173
Association between esophageal baseline impedance levels and reflux parameters suggesting defensive mechanisms
Sang Hyuk Jung and Seon-Young Park
Chonnam National University, Gwangju, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Ambulatory multichannel intraluminal impedance-pH monitoring (MII-pH) and high-resolution esophageal manometry (HRM) are commonly performed to objectively assess pathologic reflux and understand the pathomechanism in individuals with reflux symptoms. This study aimed to investigate the relationship between novel MII-pH parameters and other metrics in patients experiencing refractory reflux symptoms.
Methods: This retrospective study included patients with persistent reflux symptoms who underwent both HRM and MII-pH assessments. We evaluated total acid exposure time (AET), total bolus exposure time (BET), total number of reflux episodes, post-reflux swallow-induced peristaltic wave index (PSPW-I), and mean nocturnal baseline impedance (MNBI) from MII-pH data. The mean distal contractile integral (DCI) during swallows was derived from HRM.
Results: In this cohort of 55 patients (30 females, median age 58 years), 9 (16.4%) exhibited AET >6.0%, 16 (29.1%) experienced >80 reflux episodes, and 7 (12.7%) had ineffective esophageal motility. Median (10%–90%) MNBI values for Z5 and Z6 were 2140 (258–4046) ohms and 1680 (158–3994) ohms, respectively. Median PSPW-I (10%–90%) was 0.31 (0.07–0.59). MNBI at Z5 and Z6 showed positive correlations with DCI and negative correlations with ineffective swallows, AET, BET, acid clearance time, bolus clearance time, and total reflux episodes (P<0.05). PSPW-I exhibited positive correlations with DCI and negative correlations with ineffective swallows, AET, BET, total reflux episodes, and bolus clearance time (P<0.05). Additionally, MNBI values at Z4, Z5, and Z6 correlated with PSPW-I (P<0.05).
Conclusions: MNBI and PSPW-I are associated with both the quantitative reflux index and reflux parameters suggesting defensive mechanisms.
PP-01-174
Long-term outcomes after therapeutic induction in patients with functional dyspepsia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background and Aims: Quality of life (QOL) of patients with functional dyspepsia is known to be impaired. However, the long-term outcome in gastrointestinal symptoms and QOL after therapeutic induction in patients with FD is unclear. We aimed to clarify long-term outcome including gastrointestinal symptoms and QOL in patients with FD after therapeutic induction.
Methods: We enrolled 111 FD patients whose clinical data were traceable for 2 years after therapeutic induction. Gastrointestinal symptoms and QOL were assessed using GSRS, HADS and SF-8 questionaries before and after treatment.
Results: The improvement rate of symptoms in patients with FD was 55% and 78% at 1 or 2 years after treatment, respectively. After 1-year treatment, the initial diarrhea score in GSRS and depression score in HADS before treatment were significantly higher in FD patients without improvement than in those with improvement (p < 0.05), whereas SF-8 did not differ between two groups. After 2 years of treatment, the initial GSRS including diarrhea, abdominal pain and dyspepsia and initial total HADS score before treatment were significantly higher in FD patients without improvement than in those with improvement (p < 0.05). In addition, physical component summary on SF8 was significantly lower in FD patients without improvement.
Conclusions: Only half of patients with FD achieve complete symptom improvement at after 1-year treatment. The patients who had high HADS scores and GSRS (diarrhea and abdominal pain) showed no significant improvement in their gastrointestinal symptoms.
PP-01-175
Bile Reflux Gastropathy in Adult Filipinos with Dyspepsia
Ronell Lee and Tan
Chinese General Hospital And Medical Center, Manila, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Bile Reflux Gastropathy in Adult Filipinos with Dyspepsia
LEE Ronell; TAN Jose
Chinese General Hospital and Medical Center
Objectives: This study aims to determine the prevalence of Bile Reflux Gastropathy (BRG) among adult Filipinos with dyspepsia. Secondary objectives include comparing endoscopic findings between patients with and without BRG, associating BRG presence with patient symptoms, and identifying risk factors for BRG.
Materials and Methods: This analytical cross-sectional prevalence study will include adult Filipinos with dyspepsia scheduled for Esophagogastroduodenoscopy (EGD) at the Endoscopy Unit of Chinese General Hospital. Patients aged 19 and older meeting Rome IV criteria for functional dyspepsia will be included. Exclusion criteria will comprise patients with previous stomach, gallbladder, or small bowel surgery and those unable to communicate effectively. Consecutive sampling will be employed until the sample size of 176 is reached. Data will be collected from pre-procedural endoscopy forms, endoscopy reports, images, and videos. Statistical analyses will include Pearson’s chi-squared test and independent t-test with a significance level set at p ≤ 0.05.
Results: It is anticipated that BRG will be identified in a significant proportion of the 176 patients. Patients with BRG are expected to exhibit more severe endoscopic findings and symptoms such as postprandial fullness and epigastric pain. Anticipated risk factors for BRG include NSAID use and Helicobacter pylori infection.
Conclusion: The anticipated prevalence of BRG in adult Filipinos with dyspepsia is notable, with expected significant correlations between BRG, endoscopic findings, and patient symptoms. Identifying BRG is crucial for tailored management strategies to improve patient outcomes.
PP-01-176
Associations of Atrophic Gastritis and Intestinal Metaplasia with Refractory/Recurrent Epigastric Pain-A Retrospective Cohort Study
Chan Yi Lin1, Jyh-Ming Liou2 and Tzu-chan Hong2
1National Taiwan University Hospital, Taipei, Taiwan;2Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital Cancer Center, Taipei, Taiwan, Taipei, Taiwan;3Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital Cancer Center, Taipei, Taiwan, Taipei, Taiwan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to explore the correlation between the severity of gastric atrophy (GA) and gastric intestinal metaplasia (GIM) and the occurrence of symptoms, particularly refractory and recurrent epigastric pain.
Materials and Methods: Data from 431 patients who underwent endoscopy for upper GI symptoms at the National Taiwan University Cancer Center from January 2022 to December 2023 were analyzed. Clinical characteristics, symptoms, endoscopic findings, and pathology results were collected, including refractory or recurrent pain post-acid suppression therapy. GA and GIM were assessed using the OLGA and OLGIM grading systems. Statistical analyses included Pearson’s Chi-Square and Kruskal-Wallis ANOVA, with a significance threshold of p < 0.05.
Results: The mean age was 62.6 years, with 48.3% male. Epigastric pain occurred in 59.2% of patients, with 15.1% reporting refractory pain and 6.2% having recurrent pain. Increased GA and GIM severity correlated with older age. GIM (OLGIM stage 1-4) was associated with more symptoms (p = 0.0143) but not recurrent pain. GA (OLGA stage 1-4) was linked to recurrent pain (p = 0.0208) but not refractory pain. Multivariable analysis found GIM associated with age (OR 1.10, p = 0.0011) and H. pylori infection (OR 7.15, p = 0.0013), while GIM severity was linked to refractory pain (OR 16.11, p = 0.0189).
Conclusion: This study highlights significant correlations between GA, GIM, and symptoms. Refractory pain was associated with GIM severity, while recurrent pain was linked to GA severity. These findings underscore the importance of considering gastric pathology in symptom evaluation and early detection strategies.
PP-01-177
COMPARATIVE STUDY OF GASTROINTESTINAL GAS VOLUME IN PATIENTS WITH FD BASED ON CT AND GVS
Cong Liu, Doctor Bin Whang, Doctor Lili Zhang, Doctor Wei Zhao, Doctor Hong Jin, Shiwei Ru, Shujin Li and Xuechai Liu
Tianjin Medical University General Hospital, Tianjin, CN, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To compare the efficacy of the gas volume score (GVS) and computed tomography (CT) imaging combined with a calculation formulain detecting gastrointestinal gas volume among patients with functional dyspepsia (FD).
Materials and Methods: December 1, 2021 to June 30, 2022, 27 FD patients, as the FD group, and 30 healthy individuals, as the control group were admitted from Tianjin Medical University General Hospital from. Both groups underwent X-rays and CT scans, with subsequent evaluation of gastrointestinal multi-site gas volume using the GVS and CT methods. Gastrointestinal multi-site gas volume of the different subtypes of FD were also compared.
Results: Based on the GVS,small intestine and colorectal gas volumes were higher in the FD group than the healthy control group (P = 0. 001 and <0. 001). But,there were no significant differences in the gas volume among different sub types. Based on the CT method, the gas volume in the stomach and colon of the FD group was higher than that of the healthy control group, and the differences were statistically significant (P=0.006, 0.001); In addition, PDS patients exhibited higher gastric gas volume than EPS patients (P=0.038), which was not seen by GVS.
Conclusion: The amount of gastrointestinal gas in FD patients is higher than that in control group. The gas accumulation in the gastric cavity of PDS patients is significantly greater than that in EPS patients. The CT method can help to calculate the gas volume in the gastrointestinal tract of FD patients more accurately.
PP-01-178
Nonsteroidal Anti-inflammatory Drug Gastropathy: Correlation between The Degree of Gastric Mucosal Injury and Endoscopic Appearances
Meti Metiani1, Dolvy Girawan2, Muhammad Begawan Bestari3, Nenny Agustanti4 and Eka Nugraha5
1Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;2Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;3Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;4Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;5Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: We aim to assess the correlation between the degree of gastric mucosal injury, as determined by endoscopic examination (Lanza score) and histopathological examination (Sydney classification), while also considering the impact of patient characteristics.
Materials and Methods: From January 2022 to June 2024, we conducted a retrospective cross-sectional study, sampling a total of 386 adult patients (over 18 years of age) who had symptoms of dyspepsia or upper gastrointestinal bleeding, were taking NSAIDs and underwent endoscopic procedures and biopsy. We classified endoscopy results using the Lanza score. Histopathology results were categorized by the Sydney classification. We conducted statistical analysis using the chi-square and Spearman correlation tests.
Results: There was a positive correlation between the Lanza score and the degree of inflammation (r=0.198; p<0.001), neutrophil activation (r=0.208; p<0.001), glandular atrophy (r=0.173; p=0.001), and intestinal metaplasia (r=0.096; p=0.058). However, it did not correlate with the density of H. pylori infection (r=0.014; p=0.783). The higher the patient's age category, the more severe the injury with the Lanza score (r=0.156; p<0.05) and the greater the possibility of experiencing an ulcer (r=0.068, p=0.180), but increasing age did not have a significant effect on the Sydney classification. Gender did not affect gastric mucosal injury, whether assessed by the Lanza score or Sydney classification.
Conclusion: The more severe the injury to the gastric mucosa, the greater the degree of inflammation. Increasing age can also affect the severity of mucosal abnormalities, but neither gender nor the H. pylori infection influence their severity.
PP-01-179
Vitamin D Supplementation Improving Stress Level, Autonomic Nervous System,and Inflammatory Markers in Functional Dyspepsia
Ari Prasetyo Nugroho, Triyanta Yuli Pramana, Aritantri Darmayani and Didik Prasetyo and K. Psi Ratih Arianita
Rsud Dr.moewardi Surakarta, Surakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Impairment in gut-brain communication is one of the main causes functional dyspepsia (FD). FD might be associated with autonomic dysfunction, which can be identified via heart rate variability (HRV). It has been suggested that FD may be associated with decreased levels of vitamin D. Recent studies demonstrated that vitamin D supplementation improved depression and anxiety, which are common psychological comorbidities in patients with FD. Therefore, we investigated whether vitamin D supplementation effectively improved the FD symptoms, ANS imbalanced, and inflammatory markers in patients with FD.
Method: This is an experimental study with a one-group pretest and post-test design. FD scoring by the NDI scale and stressor scale scoring with STAI-S and STAI-T. Inflammatory markers (NLR, RDW, and PLR) and HRV were performed in all subjects before and after vitamin D supplementation for 1 month.
Results: The study, which included 25 patients, 20 women and 5 men showed there was a significant effect of vitamin D on reducing the NDI score in FD patients (p < 0.001). The study also showed a decrease in the STAI stressor level and an improvement in the SDNN with p < 0.001, and there was an increase in the LF/HF value, but was not significant. Vitamin D reduced inflammatory markers, especially NLR, PLR, and RDW (p = 0.183, p = 0.626, p = 0.740), respectively. However in this study, the decrease in these values was not significant.
PP-01-180
Vitamin d play a role in stabilizing the level of stress and sympathovagal reflex balance
Triyanta Yuli Pramana1, K. Psi Ratih Arianita2, Aritantri Darmayani1, Didik Prasetyo2, Apriliana Adhyaksari3 and Ari Prasetyo3
1Division Of Gastroenterohepatology Internal Medicine Department, Faculty Of Medicine Sebelas Maret University Dr. Moewar, Surakarta, Indonesia;2Division of Psychosomatic and Palliative Medic Internal Medicine Department, Faculty of Medicine Sebelas Maret University Dr. Moewardi Hospital, Surakarta, Surakarta, Indonesia;3Subspecialist Program Division of Gastroenterohepatology Internal Medicine Department, Faculty of Medicine Sebelas Maret University Dr. Moewardi Hospital, Surakarta, Surakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Functional dyspepsia (FD) is a common functional disease that includes a disorder of gut-brain interaction. Psychosocial factors are considered to play an important role and can also be associated with autonomic nervous system (ANS) dysfunction. Vitamin D is found to be important not only for physical health problems but also to address various mental health issues, especially reduction in the occurrence of negative emotions. Vitamin D also has immunomodulatory properties that might be useful in FD. In this research, we want to prove the efficacy of vitamin D in stress level improvement and sympathovagal reflex balance.
Methods: A total of 25 FD subjects underwent an HRV examination to record the changes of sympathovagal activity. The ANS balance is described through RMSSD, Total Power (TP) score, and PSI. Stress Level was measured by PSS-10. All examinations are carried out before and after being given vitamin D on all subjects. All data was analyzed using Wilcoxon Rank Test and Paired t-test.
Results: Vitamin D had a significant effect on improving PSS-10 score (P<0.001) which indicated a decrease stress levels in FD patients. Vitamin D also improved HRV levels which was described by a decrease in PSI (P<0.001); increase in TP (P<0.001) and increase in RMSSD (p<0.001).The ANS stability also decreased, indicating there was a better balance was achieved (p=0.002) and the activity of ANS also increased (p=0.028).
PP-01-181
Correlation between Lifestyle and Food Preferences with Gastritis Diagnosis Based on Endoscopic Results in Makassar
Muhammad Azhary Eka Putra1, Fardah Akil2,3, Susanto Hendra Kusuma2,3, Muhammad Luthfi Parewangi2,3, Nu'man AS Daud2,3, Rini Rachmawarni Bachtiar2,3 and Amelia Rifai2,3
1Internal Medicine of Hasanuddin Univerisity, Makassar, Indonesia;2Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Hasanuddin University, Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia;3Centre of Gastroenterology-Hepatology HAM Akil, Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Gastritis is a common gastrointestinal disorder characterized by inflammation of the stomach lining, leading to symptoms such as abdominal pain, discomfort, nausea, and loss of appetite. The role of lifestyle and dietary habits in the development and management of gastritis is crucial but not fully understood. This study aims to examine the correlation between lifestyle and dietary habits with diagnosis of gastritis based on endoscopic results, identifying significant dietary factors that influence the likelihood of developing various types of gastritis.
Materials and Methods: A cross-sectional analysis was conducted using medical record data and endoscopic reports. Logistic regression was used to analyze the relationship between six independent variables—fiber intake, fatty food intake, alcohol consumption, NSAID overused, and smoking—and diagnose of gastritis based on European Society of Gastrointestinal Endoscopy (ESGE) Consensus. Data preprocessing included normalizing variables on a 0-100 scale to 0-1 and encoding binary variables.
Results: This study involved 92 patients with various clinical manifestations of gastritis who had undergone endoscopy. There are 49 male patient and 43 female patient. Significant associations were found between dietary habits and predictor diagnostic of gastritis. Fiber intake had varied impacts on different gastritis types. Alcohol consumption and NSAID use were positively correlated with increased gastritis risk. The logistic regression models showed moderate to good performance, with varied influences of dietary fats and smoking on gastritis types.
PP-01-182
Role of Psychological Distress in Functional Dyspepsia: Relationship between NDI, HADS,PSS-10 and ANS
Stefanus Ranty, Triyanta Yuli Pramana, K. Psi Ratih Arianita, Aritantri Darmayani and Didik Prasetyo
RS Dr. Moewardi, Surakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Functional dyspepsia (FD) defined as chronic or recurrent upper abdominal pain or discomfort without any structural abnormalities in the gastrointestinal tract. Psychological distress is understood to be associated with FD, and both distress and anxiety can precede symptoms and the symptoms can induce stress and anxiety. This study aims to evaluate the relationship between the degree of severity of FD, measured by NDI, and degree of psychological factors, calculated by HADS and PSS-10, and also between NDI and imbalance of ANS's activity (SNS and PNS, PSI, ANS balance and ANS activity, measured with Heart Rate Variability (HRV))
Materials and Methods: This is a cross-sectional study, involving 25 FD patients, filling the NDI criteria, HADS, and PSS-10. Heart rate variability was measured, and all variables needed were collected, all in one time meeting. All data was analyzed, using Correlation Eta test, Spearman Rank and/or Pearson Product Moment.
Results: There is positive correlation between NDI and PSS-10 (r=0.515, p=0.008) and NDI with HADS-A (r=0.542, p=0.005), while no significant correlation with HADS-D (r=0.327, p=0.111). the NDI is also not correlated with SNS&PNS, PSI, ANS Balance, and ANS activity. (r=0.099, p-0.638; r=0.041, p=0.846; r=0.164, p=0.434; r=-0.096, p=0.647 respectively
PP-01-183
Significance related diseases of gastric intestinal metaplasia in children and adolescents: preliminary survey
Eell Ryoo
Gachon University, Gil Medical Center, Incheon, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Gastric intestinal metaplasia (GIP)is recommended for regular examination as precancerous lesions associated with increased risk of gastric cancer in adults, but there are few studies related to this in children and adolescents. Through a preliminary study, the prognosis was to be followed up for an observable period.
Method: From 2001 to July 2024, patients who visited the hospital due to gastrointestinal symptoms and performed gastric biopsy along with upper gastrointestinal endoscopy were analyzed retrospectively.
Results: Of 7,979 patients (65,733 cases), 3,533 patients (7,707 cases; 39.3%) underwent upper gastrointestinal endoscopy and biopsy.
Of histologic findings, 31 cases (0.88%) showed GIP, 14 cases were male, the mean age was 13.01±3.70 years old, and the BMI was 19.2±3.3 kg/m2. The chief complaints were abdominal pain in 25 cases, nausea or vomiting in 4, and abdominal discomfort in 2. As a result of endoscopy, 10 erosive gastritis, 6 hemorrhagic gastritis, 4 gastric and duodenal ulcer, 4 reflux esophagitis, 3 normal, 2 bile reflux gastritis, 1 polyps, and 1 nodular gastritis. Of these, H. pylori positive was 23% (7 cases). The final diagnosis was 9 acute gastritis, 7 H. pylori gastritis, 4 reflux esophagitis, 3 gastric and duodenal ulcer, 3 functional gastrointestinal disease, 2 Crohn's disease, 2 bile reflux gastritis, and 1 tubular adenoma.
There were 15 patients who underwent follow-up endoscopy, and in two of these cases, intestinal metaplasia persisted on follow-up examination.
Conclusion: GIP is very rare in children and adolescents and seems to be highly related to H. pylori infection.
PP-01-184
Effective Treatment of Chronic Atrophic Gastritis with Umbilical Cord Mesenchymal Stem Cells Submucosal Injection
Qianqian Xu, Mingyue Liu and Hongwei Xu
Department of Gastroenterology, Shandong Provincial Hospital, Shandong, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate the therapeutic effect of endoscopic submucosal injection of human umbilical cord mesenchymal stem cells (hUC-MSCs) on chronic atrophic gastritis (CAG) in rabbits and investigate the impact of hUC-MSCs on atrophy using a co-culture system.
Materials and Methods: HUC-MSCs were isolated from human umbilical cord tissue, characterized by morphology, surface markers, and differentiation abilities. CAG in rabbits was induced using MNNG, ammonia, sodium salicylate, and ranitidine. After 12 weeks, endoscopic examinations, biopsies, and serum markers (G-17, PG I, PG II, PG I/II) were assessed. HUC-MSCs were submucosally injected via endoscopy; controls received saline. Treatments were administered twice, 4 weeks apart, with evaluations on days 30 and 60 post-initial injection. CAG cell model (MC cells), induced using MNNG-stimulated GES-1 cells, were co-cultured with hUC-MSCs in a Transwell co-culture system. Apoptosis was assessed by flow cytometry, autophagosomes by immunofluorescence, and protein markers of EMT, apoptosis, and autophagy by Western blot.
Results: HUC-MSCs showed spindle-shaped morphology, expressed MSC markers, and differentiated into adipogenic, osteogenic, and chondrogenic lineages in vitro. Model rabbits exhibited significant gastric mucosal changes and decreased serum markers. HUC-MSCs injection increased serum marker levels, improved gastric mucosa appearance, alleviated glandular atrophy, restored mucosal structure, and inhibited inflammation. Saline controls showed no significant improvement. Furthermore, HUC-MSCs inhibited apoptosis and EMT in MC cells and promoted autophagy.
PP-01-185
Two Cases of Endoscopic Antireflux Mucoplasty (ARMP) Using the Reopenable-Clip Over the Line Method (ROLM)
Satoshi Asai, Kento Hisamatsu, Yuma Fujita and Kotaro Takeshita
Dep. Gastroenterology, Tane General Hospital, Osaka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Endoscopic antireflux mucosectomy (ARMS) for patients with proton pump inhibitor (PPI)-refractory gastroesophageal reflux disease (GERD) has been covered by the national insurance system in Japan since 2022. ARMP is a procedure in which the post-ARMS ulcer is closed using an endoscopic closure technique. ARMP is expected to reduce post-procedure hemorrhage and provide more rapid symptom improvement compared to ARMS. However, endoscopic closure of post-ARMS ulcers is technically demanding due to the challenging maneuverability of the endoscope.
Case Description: Case 1: A fifty-four-year-old female with a history of sleeve gastrectomy was suffering from PPI-refractory regurgitation. Esophagogastroduodenoscopy (EGD) revealed reflux esophagitis (LAclassification M), 2 cm cardiac opening (CO), and 3 cm sliding hernia (SH). pH monitoring on PPI indicated frequent reflux and associated symptoms. Therefore, ARMP using the ROLM was performed (procedure time: 74 minutes). The patient's symptoms improved immediately after the procedure without any adverse events.
Case 2: A seventy-five-year-old male was suffering from PPI-refractory regurgitation and heartburn. EGD revealed reflux esophagitis (LA classification C), 2.5 cm CO, and 2 cm SH. pH monitoring on PPI showed values at the upper limit of the reference range. Given his significant reflux symptoms and desire for the procedure, ARMP using the ROLM was performed (procedure time: 60 minutes). The patient's symptoms partially improved immediately after the procedure without any adverse events.
Discussion: ARMP using the ROLM was relatively easy to perform and is considered feasible.
PP-01-186
Our Experience of Anti-Reflux Mucosectomy; ARMS for Refractory Gastroesophageal Reflux Disease
Yoshitaka Hata1,2, Hiroki Fukuya1, Hirotaka Tsuru1, Masafumi Wada1, Bai Xiaopeng1, Yosuke Minoda1, Yoshimasa Tanaka1, Haruei Ogino1 and Eikichi Ihara1
1Kyushu University, Fukuoka, Japan;2Saiseikai Futsukaichi hospital, Cikushino city, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Backgrounds: Gastroesophageal reflux disease (GERD) is mainly caused by gastric acid reflux, and the primary treatment is acid secretion inhibitor such as proton pump inhibitor (PPI). Recently, a strong acid secretion inhibitor, vonoprazan, has been developed, but a subset of patients exhibits refractory GERD, which presents a clinical challenge. The pathogenesis of refractory GERD involves insufficient acid secretion suppression, non-acid reflux, esophageal hypersensitivity and altered esophageal motility. Therefore, multichannel intraluminal impedance/pH monitoring (MII/pH) and high-resolution manometry (HRM) are indispensable to evaluate refractory GERD. In addition, endoscopic anti-reflux therapy such as anti-reflux mucosectomy (ARMS) has been developed and is anticipated to offer another promising treatment for refractory GERD in Japan.
Methods: This study aimed to evaluate the safety and effectiveness of ARMS in introduction phase. Seven patients who underwent ARMS for refractory GERD in Kyushu university hospital between April 2022 and March 2024 were included. Patients' pre- and post-ARMS symptom scores and examination results were evaluated.
Results: Median age was 68 years with male to female ratio 4:3. Six patients were administered vonoprazan and one patient were administered PPI. All patients exhibited cardiac opening with MII/pH identifying 4 with non-erosive reflux disease and 3 with reflux hypersensitivity. The ARMS procedures were successfully completed in all patients, with the F-scale improving from 15 to 7. MII/pH also demonstrated a reduction in reflux frequency and lower esophageal acid exposure time.
Conclusion: ARMS can be an effective treatment for refractory GERD, after excluding functional heartburn and esophageal motility disorders not associated with reflux.
PP-01-187
Erosive Esophagitis Status in A High-Risk Area of Gastric Cancer in Indonesia
Ryan Herardi1,2, Ari Fahrial Syam3, Muhammad Miftahusurrur4, Hasan Maulahela3, Agustinus Taolin5, Rasco Sandy Sihombing5, Ahmad Yusran1, Gaberia Gaberia5, Nathaniel Jason Zacharia5, Iqbal Taufiqqurachman6 and Astried Monica7
1Gastroenterohepatology Fellowship. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;2Faculty of Medicine, UPN Veteran Jakarta, Jakarta, Indonesia;3Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy. Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;4Division of Gastroenterohepatology. Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;5Mgr. Gabriel Manek Hospital, Atambua, Belu, Indonesia;6Research Staff. Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;7Research Staff. Department of Internal Medicine, Faculty of Medicine, UPN Veteran Jakarta, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Helicobacter pylori infection (HPI) is a high-risk factor for gastric cancer. This study aims to assess the prevalence of erosive esophagitis in populations with a high rate of HPI and evaluate their associations
Materials and Methods: A cross-sectional study was conducted at Mgr Gabriel Manek Hospital, Atambua, Belu, East Nusa Tenggara, Indonesia from May 27th to June 8th, 2024. Patients with persistent upper gastrointestinal discomfort (>3 months) were included. All participants ceased consuming proton pump inhibitors, H2 antagonists, Non-Steroid Anti Inflammation Drugs, and steroids for at least four weeks before the examination. Esophagogastroduodenoscopy and Urea Breath Test were performed to diagnose erosive esophagitis and HPI. The study excluded pregnant patients and those with autoimmune disease, immunosuppression, kidney disease, biliary disease, hepatic cirrhosis, or upper gastrointestinal obstruction. A chi-square analysis examined the association between erosive esophagitis and HPI.
Results: A total of 117 patients were assessed, with 65 (55.6%) female. Of these, 60 patients (51.3%) were within 20 to 40 years. Among these patients, 37.6% presented with erosive esophagitis, and 41% of patients were found to have HPI. The prevalence of erosive esophagitis was categorized as follows: grade A in 35 patients (29.9%), grade B in 8 patients (6.8%), and grade C in 1 patient (0.9%). A chi-square analysis revealed a p-value of 0.683, indicating no significant association between erosive esophagitis and HPI.
Conclusion: Despite a high Helicobacter pylori infection rate, there is no protective correlation with the prevalence of erosive esophagitis in this rural area.
PP-01-188
Esophageal Adenocarcinoma in Long Segment Barrett’s Esophagus extending ≥10 cm
博士 Tomohiro Inoue and Mamoru Ito and Masao Yoshioka and Satsuki Yoshinari and Ryouichi Harada and Daisuke Kawai and Keita Harada and Shuhei Ishiyama and Akiko Fujiwara and Junichirou Nasu and Junji Shiode
Okayama Saiseikai General Hospital, Dept. of Gastroenterology, Okayama, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: ESGE guidelines recommend 3-year surveillance for Barrett's esophagus (BE) of 3-10 cm and referral to specialty centers for BE ≥10 cm. Discontinuation of surveillance is suggested for patients ≥75 years. In Asia, adenocarcinoma is relatively rare compared to squamous cell carcinoma. In Japan, targeted biopsy is more common due to the widespread use of magnifying endoscopy, and surveillance is often done at gastroenterology clinics rather than specialized centers, with an average lifespan of 84 years. Uniform application of European standards may be challenging in this context.
Materials and Methods: This study evaluated ESGE guidelines by analyzing esophageal adenocarcinoma cases from BE ≥10 cm at Okayama Saiseikai General Hospital (January 2011-March 2024). Data included patient demographics, Barrett's adenocarcinoma risk factors (GERD, dysplasia, obesity, smoking, alcohol, family history), diagnostic trigger, BE-to-adenocarcinoma interval, and maximum BE length.
Results: There were four patients. All patients were male, aged 67-92 years (median 74). The mean length of BE was 10.8 cm. Diagnoses occurred 12 years after pharyngoesophageal discomfort, 21 years after appetite loss, 8 years after surveillance, and incidentally at the first screening respectively. Risk factors: GERD (4/4), dysplasia (1/4), obesity (1/4), smoking (1/4), alcohol (1/4), and family history (0/4).
Conclusion: For high-risk patients with BE ≥10 cm, BE-to-adenocarcinoma intervals varied significantly. Two patients over 75 years developed adenocarcinoma, challenging the guideline for discontinuation of surveillance. Targeted biopsies may ease the burden on elderly patients. Long-term surveillance is crucial for LSBE, and further research is needed to refine surveillance methods, intervals, and age limits.
PP-01-190
Relationship brtween oral acid or burning sensation and gastroerophageal reflux
Cong Liu1, Doctor Lili Zhang1, Doctor Wei Zhao1, Doctor Bin Whang1, Doctor Hong Jin1, Xuechai Liu1, Shujin Li1, Shiwei Ru1 and Jinfeng Zhang2
1Tianjin Medical University General Hospital, Tianjin CN, China;2Tianjin Fourth Cent Hosp, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the relationship between oral acid/burning sensation and gastroesophageal reflux disease(GERD) using salivary pepsin detection.
Materials and Methods: From May to June, 2023, 116 patients were enrolled consecutively at the endoscopy center of Tianjin Medical University General Hospital. 20 patients with oral acid/burning sensation (symptom group) and 96 patients without oral acid/burning sensation (control group) underwent a symptom questionnaire survey, salivary pepsin detection, and electronic gastroscopy. Salivary pepsin positivity, the prevalence of GERD, and scores of anxiety and depression were compared between the two groups.
Results: 17.2% patients suffered from oral acid/burning sensation. Salivary pepsin test positivity and GERD Q scores were significantly higher in the symptom group than in the control group(80% vs. 41.67%, P<0.05), (11 (6~12) vs. 6 (6~6), P<0.001). While, in symptom group the prevalence of reflux esophagitis was marginally higher than that in control group.(P>0.05). By subgroup analysis, a significantly higher positive rate of salivary pepsin was observed in non-elderly group, compared to the elderly group. (57.14% vs 25%, P = 0.002).Furthermore, among patients in the symptom group, those with a salivary pepsin negative demonstrated higher levels of anxiety and depression compared to those with a salivary pepsin positive. (P<0.001). (Figure 1)
Conclusion: Oral acid or burning sensation might be caused by gastroesophageal reflux. Age, together with anxiety, and depression might also be influencing factors . Salivary pepsin test might be helped to find the mechanism of oral acid or burning sensation.
PP-01-191
Evaluation of diagnostic test and usefulness of plain chest X-ray in esophageal achalasia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Esophageal motility disorders including achalasia are sometimes difficult for diagnosis especially in early stage or lacking dilated esophagus. They decrease QOL and increase risk of esophageal cancer, so early diagnosis is important. Therefore, we evaluated the test and modality for detecting achalasia.
Material and methods: We retrospectively reviewed 258 patients who were performed POEM at our institute between Apr 2015 and DEC 2023, and investigated their plain chest X-ray for detecting air column beside the trachea.
Results: 120 cases were female and 138 were male. Median age at diagnosis was 44 (8 to 85). Median duration of symptom was 31.5 (0-593) months. There were 153 Chicago Classification type1,29 type2 and 19 type3 cases. 11 cases were other motility disorders. 92 cases were gradeI, 145 were gradeII and 21 were gradeIII dilation. There were 194 straight type and 64 sigmoid type cases. Diagnostic accuracy in each test were as follows. Barium radiography was 88% (22/25 cases), CT was 87% (14/16 cases), and endoscopy was 52% (57/109 cases) in clinic or hospital where they visited first. 145/254 cases (57%) could be detected by plain chest X-ray when we retrospectively reviewed. Among 52 patients who were missed by previous doctor’s endoscopy, 29 cases could be detected by X-ray.
Conclusion: Achalasia can be easily missed by endoscopy. Plain chest X-ray is sometimes useful for detecting achalasia, so using both X-ray and endoscopy is effective especially in clinic where barium radiography or CT scanner isn’t available.
PP-01-192
Vonoprazan, the Answer of Unmet Needs GERD Therapy : A Systematic Review and Meta-Analysis
Mario Steffanus1,2, Riki Tenggara1, Angelina Yuwono1, Hasan Maulahela3 and Dewa Nyoman Wibawa4
1Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia;2Division of Gastroenterology and Hepatology Trainee, Department of Internal Medicine, Faculty of Medicine of Universitas Indonesia, Jakarta, Indonesia;3Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy Department of Internal Medicine Faculty of Medicine, Universitas Indonesia Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;4Division of Gastroenterology and Hepatology, Departmen of Internal Medicine, Udayana University, Denpasar, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Proton pump inhibitors (PPIs) have traditionally been the cornerstone for treating gastroesophageal reflux disease (GERD). Despite their widespread use, several unmet needs remain, including issues related to non-erosive reflux disease, severe erosive esophagitis, post-meal heartburn, refractory GERD, atypical and extraoesophageal GERD presentations, Barrett’s oesophagus, and GERD following bariatric surgery. Vonoprazan, a potassium-competitive acid blocker, has shown promising acid-suppressing properties and has been used in GERD management. This systematic review evaluates Vonoprazan's effectiveness in addressing the unmet needs of GERD therapy.
Material and Methods: Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines, this systematic review assesses Vonoprazan's efficacy in alleviating GERD symptoms compared to PPIs. Databases such as PubMed, ProQuest, and Google Scholar were utilized for this review. A meta-analysis was also performed to evaluate oesophageal healing.
Results: Eighteen clinical trials, including nine randomized controlled trials (RCTs), were analyzed. Most studies indicated that Vonoprazan significantly alleviated reflux symptoms, as measured by the frequency scale for the symptoms of gastroesophageal reflux disease (FSSG) and the GERD-Questionnaire (GERD-Q) scores. Improvements were also observed in night-time symptoms (Pittsburgh Sleep Quality Score / PSQI score), mucosal healing, and gastric pH levels. Three studies were eligible for meta-analysis, demonstrating better mucosal healing in the Vonoprazan group (p Value: 0.04, OR 3.07, 95% CI 1.05-9.9).
Conclusion: Vonoprazan is better than PPIs and shows potential for addressing the unmet needs in GERD treatment.
Keywords: vonoprazan, reflux symptoms, effectiveness, unmet need therapy
PP-01-193
Association between fagerstorm test nicotine dependence and interleukin-8 with esophagitis grade in smoker’s gerd subjects
Finly Septianto1, Titong Sugihartono1, Budi Widodo1, Herry Purbayu1, Annisa Zahra Mufida1,2 and Tri Asih Imroati2
1RSUD dr Soetomo, Surabaya, Indonesia;2Airlangga Teaching Hospital, Surabaya, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Gastroesophageal Reflux Disease (GERD) is defined as a pathological condition as a result of reflux of stomach contents into the esophagus which causes various disturbing symptoms in the esophagus and extra esophagus, and one of the causes is a poor lifestyle such as smoking. This recurrent reflux condition can lead to systemic inflammation and it can be affectedby IL-8 and IL-6. Esophagitis is the most frequent complication. Elevated levels of IL-8 have been associated with disease progression, clinical severity, and severity of esophagitis in GERD patients. Aim. To determine the role of smoking severity and IL-8 levels in the development oesophagitis in smoker GERD subjects.
Material and Method: This research is a cross-sectional analytic study at dr. Soetomo General Hospital, Surabaya. All the subjects were smokers, that consist of 28 male and 10 females. The severity of smoking was assessed by the Fagestorm Test Nicotine Dependence (FTND) questionnaire, serum IL-8 levels were measured using ELISA, and the degree of esophagitis was assessed using esophagogastroduodenoscopy (EGD). Data analysis were done using SPSS version 22.
Result: There was a significant association between FTND and the degree of esophagitis (p = 0.037). There was no significant association between IL-8 levels and the degree of esophagitis (p = 0.697). There was no significant association between FTND and IL-8 levels (p = 0.556).
Conclusion: There was a significant association between the severity of smoking and the degree of esophagitis in smoking GERD patients.
PP-01-194
Influence of Age, BMI, Gender and Bolus Viscosity on Esophageal Contractions in Healthy Individuals
Division of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: This study explores how bolus viscosity, age, BMI and gender modulate esophageal contractions.
Methods: Healthy individuals without dysphagia (Swallow Disturbance Questionnaire score <1) underwent high-resolution esophageal impedance manometry (Medtronic Inc., MN, USA). Triplicate swallows of 5 mL liquid or one-tablespoon solid bolus were performed in supine and upright positions, following a random sequence based on the International Dysphagia Diet Standardization Initiative (IDDSI) levels 0-7. The Manoview software v3.0 (Medtronic Inc., MN, USA) analyzed swallowing parameter including lower esophageal sphincter (LES) integrated relaxation pressure (IRP), distal latency (DL), contractile front velocity (CFV), distal contractile integral (DCI), bolus transit time (BTT), and bolus entry time (BET). A linear mixed model assessed the effects of bolus viscosity, age, BMI and gender on swallowing metrics in both positions.
Results: Forty-six participants (M:F 19:27, age 51±17, BMI 22.7±2.2) were enrolled. Advancing age significantly increased IRP, DCI, BTT, and BET in both positions while CFV increased only in the supine position. Thicker bolus significantly correlated with increased in DL, DCI, BTT, and BET in both positions. Higher BMI decreased IRP in both positions. (Table1). Pearson’s correlation revealed a moderate positive correlation between IRP and DCI, with correlation coefficients of 0.37 (upright) and 0.40 (supine), p < 0.001.
Conclusions: These findings show how age, BMI, gender, bolus viscosity affect esophageal motility in healthy individuals. Older age increased IRP with stable contraction latency made swallowing thicker boluses more difficult. Further research on esophageal motility in individuals with dysphagia could enhance our understanding of these adaptations
PP-01-195
Effectiveness of Vonoprazan in Chinese Reflux Esophagitis Patients with Hiatal Hernia: a Real-world, Observational study
Yinglian Xiao1, Yiyang Dai2, Dean Tian3, Qi Song4, Li Xie4 and Minhu Chen1
1The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;2The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu, China;3Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;4Takeda Pharmaceutical Company, Shanghai, China
Objectives: Reflux esophagitis (RE) was closely related to hiatal hernia (HH). HH patients are more likely to have reflux symptoms like regurgitation and heartburn, with increasing acid exposure and prolonged acid clearance time. Thus, we aimed to investigate the effectiveness of vonoprazan in RE patients with HH.
Materials and Methods: This was a subgroup analysis from VIEW (NCT04501627) study. All patients received vonoprazan 20mg orally q.d. for 4 weeks (8 weeks if insufficient benefit) with two-week safety follow-up. Symptom relief was assessed by diary of typical symptoms and GERD Questionnaire (GerdQ).
Results: 96 RE patients with HH were evaluated, of which 79% (76/96) had HH <2cm and 21% (20/96) ≥2cm. After first one-week of treatment, the percentages of complete relief from heartburn, night-time heartburn, regurgitation, and night-time regurgitation in patients with HH ≥2cm were 30.8%, 61.5%, 46.2%, and 53.8%, while those with HH <2cm were 33.3%, 43.1%, 28.6%, and 36.0%, respectively. Higher percentages were observed in patients with HH ≥2cm with complete relief from night-time heartburn, regurgitation or night-time regurgitation. The similar trend was observed after first two-week of treatment. From baseline to week 4, percentage of patients without GERD-typical symptoms increased by 52.9% (95%CI: 28.73, 77.06), 33.4% (95%CI: 18.20, 48.53) and mean change in GerdQ score (negative values represent improvement) was -2.5 (95%CI: -3.95, -1.00), -1.6 (95%CI: -2.29, -0.83) in patients with HH ≥2cm and HH <2cm, respectively (Table 1).
PP-01-196
Safety of Vonoprazan in Chinese Patients: a Real-world, Observational Study
Yinglian Xiao1, Side Liu2, Yuping Chen3, Qi Song4, Li Xie4 and Minhu Chen1
1The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;2Nanfang Hospital, Southern Medical University, Guangzhou, China;3Zhuhai People's Hospital, Zhuhai, China;4Takeda Pharmaceutical Company, Shanghai, China
Objectives: The safety of vonoprazan has been demonstrated in randomized controlled trial but less commonly reported in the real-world. Thus, we investigated the safety of vonoprazan in real-world clinical practice in Chinese patients.
Materials and Methods: VIEW study (NCT04501627) was a multi-center, single-arm, prospective, observational, real-world study, to evaluate the safety and effectiveness of vonoprazan. Patients were treated with 20 mg vonoprazan orally q.d. for 4 or 8 weeks (if the effect is insufficient), followed by a safety follow-up for 2 weeks. Total 3000 patients were enrolled, of which patients who took at least one dose of vonoprazan and provided safety information were included in this safety analysis.
Results: Overall, 2829 patients were evaluated, of whom 87.3% (2469/2829) were non-elderly patients (aged 18-64 years) and 12.7% (360/2829) were elderly patients (aged ≥65 years). The types and frequencies of AEs were similar in the overall patients and differential age groups: 17.2% in the overall patients, 17.1% in the non-elderly patients and 18.6% in the elderly patients. The most common AEs were gastrointestinal disorders (e.g., diarrhoea, nausea), and infections and infestations (e.g., upper respiratory tract infection, gastroenteritis). The incidence of serious AEs in overall patients, non-elderly patients, and elderly patients were 1.0%, 0.8%, and 2.5%, and ADRs were 4.6%, 4.7%, and 3.9%. Most AEs and ADRs were mild or moderate.
Conclusion: This study demonstrated that vonoprazan had a favorable safety profile in Chinese patients, with a similar safety profile in non-elderly patients and elderly patients.
PP-01-197
Sleep Factors and Gastroesophageal Reflux Disease: A Study on Causality, Mediation, and Pathogenesis
Ke Tao and Hong Xu
The First Hospital of Jilin University, Changchun, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Gastroesophageal reflux disease (GERD), encompassing reflux esophagitis (RE) and non-erosive reflux disease (NERD), is a prevalent chronic condition with increasing global incidence. Sleep disturbances have been linked to various diseases. This study comprehensively evaluates the relationship between multiple sleep issues and GERD, providing a novel perspective for exploring the complex causal relationships and mechanisms.
Materials and Methods: This study utilizes data from the UK Biobank spanning from 2006 to 2010 to conduct a large-scale prospective cohort study. The impact of sleep factors on GERD incidence is examined using COX proportional hazards models, followed by Mendelian randomization to verify their causal relationships, and proteomics for intermediary analysis.
Results: COX proportional hazards models indicate that short sleep duration, difficulty waking early, late bedtime, insomnia, snoring, daytime sleepiness, and napping all contribute to the occurrence of reflux esophagitis and non-erosive reflux disease. Mendelian randomization further confirms that insomnia promotes the occurrence of RE and NERD, short sleep duration promotes RE, and difficulty waking early promotes NERD. In proteomic intermediary analysis, we further identify 19 plasma proteins mediating NERD induced by night shifts, activating seven metabolic pathways including "Intracellular oxygen transport," and 15 plasma proteins mediating RE induced by night shifts, activating two metabolic pathways including "Nuclear receptor transcription pathway."
Conclusion: Multiple sleep problems are closely associated with GERD incidence, with insomnia mediating RE and NERD through respective metabolic pathways. These findings provide new insights into GERD mechanisms and offer potential targets for the development of novel therapeutic strategies.
PP-01-198
Safety and long-term efficacy of hybrid-APC for treatment of Barrett’s oesophagus: An Australian pilot study
Rosalie Tripet
Royal Prince Alfred Hospital Sydney, Camperdown, Australie
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Radiofrequency ablation is gold-standard for dysplasia in Barrett's esophagus (BE), but has a stricture rate up to 15%. Hybrid-argon plasma coagulation (APC) was developed to reduce this risk. This pilot study evaluates the feasibility, tolerance, safety, and long-term efficacy of hybrid-APC for BE treatment.
Materials and methods: Patients with confirmed BE and dysplasia were enrolled. The procedure involved elevating the mucosa with a high-pressure water jet before thermal ablation with APC. Nodular lesions were resected by EMR. Repeat procedures were performed at 3-month intervals until complete remission of dysplasia CRD and intestinal metaplasia (CRIM). Safety assessments were conducted.
Results: Between 2017 and 2024, twenty patients (median age 68 years; 75% male) were treated with a median follow-up of over 4 years. Ten patients (56%) had a long segment of Barrett's, with low-grade dysplasia (45%), high-grade dysplasia (35%), or neoplasia (20%). Six patients (30%) required EMR. Fifteen patients (75%) achieved CRD, and twelve (67%) showed CRIM after ≤ 5 hybrid-APC treatments. Four (22%) were slow responders. There was no progression of dysplasia or cancer during the follow-up. Recurrent intestinal metaplasia (28%) or dysplasia (11%) was successfully treated with subsequent hybrid-APC sessions. No treatment-related strictures or major complications occurred.
Conclusion: This pilot study suggests that hybrid-APC is a safe, feasible, and effective long-term treatment for dysplastic BE. Close post-treatment surveillance is needed due to recurrence risks. Some patients may require > 5 hybrid-APC treatments for remission. Larger, multicenter trials are recommended to refine patient selection and optimize the hybrid-APC technique.
PP-01-199
One patient, many diseases
Dmitriy Baranov, Evgeny Solonitsyn, Valeria Kamalova, Evgeny Lebedev, Elena Malinovskaya, Dmitriy Chernikh and Ivan Danilov
V.A.Almazov National Medical Research Center (NMRC), Saint-Petersburg, Russian Federation
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Diagnosis of diseases, especially in patients with a complex comorbid background, requires increased attention, and modern technologies combined in one center improve its quality.
Case descrition: We present the case of a 51-year-old woman with a history of Crohn's disease, primary sclerosing cholangitis. She developed jaundice and abdominal pain. First hospitalization: an increase in ALT, AST, indirect bilirubin levels, endoscopic retrograde cholangiopancreatography - stent installation in common bile duct (CBD). Мagnetic resonance imaging: hepatomegaly, fibrous changes of the pancreas. A month later, pain syndrome again, hospitalization: an increase in ALT, AST, alkaline phosphatase levels; total bilirubin – the norm). A month later, the recurrence of pain syndrome, cytolysis, and an increase in the level of total bilirubin. EUS was performed: expansion of the CBD to 9 mm, intraluminal tumor masses, due to the peculiarities of blood supply, puncture was not performed. ERCP: CBD deformity, biopsy from a tumor. Morphology: adenocarcinoma.
Colonoscopy: stenosis of the descending calving of the colon. Ulcers of the sigmoid colon; SES-CD scale - 19 points. Morphology: adenocarcinoma.
Based on the received diagnostic data, the patient was determined to be treated.
Discussion: The use of various modern endoscopic techniques (EUS, ERCP with biopsy, high-resolution endoscopy and expert morphology) make it possible to correctly diagnose, determine the correct treatment tactics for the patient, and minimize the risk of clinical error.
PP-01-200
Metastatic breast cancer presenting as small bowel obstruction in adult filipino female: a case report
Jeline Symba Celocia
Baguio General Hospital And Medical Center, Bakakeng Central, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Significance: Breast cancer is the most common cancer for both sexes in the Philippines with most cases detected at an advanced stage. Very rarely does a primary breast cancer metastasize to the GI tract, even more to small bowels, specifically the ileum. Since uncommon, this poses a diagnostic challenge to the clinicians, since GI symptoms are generally nonspecific and endoscopic findings are often variable. Thus, the patient’s rare presentation demonstrates the need to consider a metastatic breast cancer among patients with bowel obstruction.
Clinical presentation: A 40-year-old-female with no comorbidities, presented at the ER with 1 month history of intermittent, crampy abdominal pain, aggravated by food intake.
Management: Endoscopy and colonoscopy revealed multiple gastric ulcers, colonic polyps and internal hemorrhoids. Patient was initially treated with H.pylori triple therapy regimen with no noted relief of symptoms. Further investigation revealed radiographic evidence of small bowel obstruction. Patient underwent exploratory laparotomy and was found out to have ileal stricture at 110cm and 120cm from ileocecal valve with multiple lymphadenopathies. Biopsy of the resected ileal segment revealed an initial consideration of lymphoma. Immunohistochemistry revealed positive for CK and CK7 stains, consistent with metastatic breast cancer. Patient then was referred to Oncology service for chemotherapy and further metastatic work – up.
Recommendation: GI symptoms are most often nonspecific in symptomatology. This should not limit a clinician with the possible differential diagnosis and should prompt the clinician to further investigate further since a GI symptom can be a presentation of an underlying metastatic disease.
PP-01-201
A case of stage IV pancreatic cancer who achieved good response to pembrolizumab treatment
Aya Kawanishi, Yoshihiro Shirataki, Masashi Morimachi, Ayano Ito, Hiroyuki Ito, Ryuzo Deguchi and Tatehiro Kagawa
Department of Gastroenterology and Hepatology, Tokai University School of Medicine, Kanagawa, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: We report a case of pancreatic cancer who achieved good response to pembrolizumab treatment.
Case description: A 62-year-old man received a CT scan examination for follow-up of an abnormal chest shadow in November 20XX. It incidentally revealed an enlarged lymph node on the lesser curvature side of the stomachHe received CT scan examinations repeatedly, which showed gradual enlargement of the gastric lesser curvature lymph node, but no primary tumors were detected. The CT scan performed in June 20XX+2 revealed a pancreatic head tumor invading the celiac artery with enlarged para-aortic lymph nodes, and the patient was referred to our hospital. We performed endoscopic ultrasound fine-needle aspiration from the lesser gastric curvature lymph node and finally made a diagnosis of stage IV pancreatic cancer. He received first-line combination therapy with gemcitabine and nab-paclitaxel. Four months later, due to the growth of the primary lesion and lymph node metastases, chemotherapy was switched to modified FOLFIRINOX. This second-line therapy was terminated 15 months later as the primary lesion had increased in size. Cancer gene panel test identified high microsatellite instability (MSI) and tumor mutation TMB 61.78 Muts/Mbp. Pebrolizumab was then started in April 20XX+3 ; six months later, the primary lesion and lymph node metastases shrank. More than 34 months have passed since the introduction of primary treatment, and treatment is ongoing.
Discussion: This report indicates that extended prognosis is possible in pancreatic cancer by utilizing early cancer gene panel test.
PP-01-202
A case of fatal jejunal lymphoma manifested by massive hemtochezia
Yujin Kim
Cheju Halla General Hospital, Jeju-si 대한민국
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Lymphoma in gastrointestinal (GI) tract can present in various symptoms, from nonspecific symptoms such as dyspepsia, abdominal pain, nausea, GI bleeding, bowel perforation or obstruction.
Case Description: A 64-year-old male patient visited emergency department room due to hematochezia one week before and dizziness. Blood tests revealed hemoglobin (Hb) level of 7g/dL. Upper GI tract was normal on endoscopy and colonoscopy showed several diverticulosis with no active bleeding. We suspected for diverticular bleeding, and hematochezia stopped after admission.
On 5th day, he passed fresh bloody stool several times, with a decrease in blood pressure of 90/56mmHg, hemoglobin 6.5g/dL, and platelet 74k/uL. Abdomen pelvis computed tomography revealed active contrast extravasation at jejunum, suggesting active bleeding. Even though emergent embolization was done, hematochezia and hemodynamic instability continued and severe anemia (Hb 5.5g/dL), ischemic liver injury, and acute kidney injury developed. We consultation for surgery and segmental jejunal resection was performed. The surgeon found multiple ulcers and variable palpable nodules.
Despite surgery, hematochezia continued and his clinical course got worse, and secondary embolization and surgery was done. But, hematochezia did not stop and he was critically ill. Despite intensive treatment, multiorgan failure progressed and he passed away on 14th day. The final pathology was proven for Extranodal NK/T cell lymphoma, nasal type.
Discussion: We conclude that bleeding from small bowel lymphoma is rare, but it should be included in differential diagnosis in patients with massive small bowel bleeding, and aggressive management including surgery is required to define the diagnosis.
PP-01-203
Microsatellite instability and EOCRC in an Emerging Economy: A Study In an Indonesian Tertiary Hospital
Saskia Nursyirwan1, Murdani Abdullah1, Nur Rahadiani2, Susanti Susanti3,4, Mohammad Ilyas5, Virly Nanda Muzellina1, Muhammad Firhat Idrus1 and Ari Fahrial Syam1
1Department of Internal Medicine Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Faculty of Medicine Universitas Indonesia / Dr. Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia;2Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia / Dr. Cipto Mangunkusumo Hospital, Central Jakarta, Indonesia;3Pathgen Diagnostik Teknologi, Ir. Soekarno Science and Technology Park, National Research and Innovation Agency Republic of Indonesia, Bogor, Indonesia;4Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah, Purwokerto, Indonesia;5Department of Pathology, University of Nottingham, Nottingham, United Kingdom
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to explore Colorectal Cancer (CRC) Microsatellite Instability (MSI) status in Indonesian patients to better understand the role of MSI in Early-onset CRC (EOCRC) rise and role of MSI in CRC screening.
Materials and methods: To achieve this, we retrieved fixed formalin paraffin-embedded samples and corresponding clinical data from 100 patients diagnosed with CRC between 2018 and 2020. DNA extraction was performed on the samples, and subsequent analysis utilized the N_lynch panel, real-time PCR, and HRM analysis. MSI status was determined based on the presence of two positive markers.
Results: As much as 12.4% of our subjects were MSI-H and 34% of the subjects were early onset cases. There is a significant difference of tumor location between MSI-H and MSS, in which larger percentage of MSI-H tumor was right-sided (33.3%) than left-sided (9.7%). No significant difference was found in terms of gender, age, onset, stage, and performance status. No significant difference between gender, tumor location, stage, MSI status, and performance status between early onset and late onset group.
Conclusion: There is a high rate of early onset CRC in Indonesia that is not caused by MSI but rather, possibly, by an undiscovered factor. Further study is needed to explore novel pathway that could explain this phenomenon.
PP-01-204
Characteristics of Gastrointestinal Adverse Events in Immune Checkpoint Inhibitors
Kento Ogawa, Yoshihide Matsumoto and Seiji Shio
Division of Gastroenterology, Shinko Hospital, Kobe, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to analyze the characteristics of ICI-induced adverse events (irAEs), focusing on gastrointestinal events.
Methods: We compared patients with gastrointestinal irAEs to those without to examine differences in cancer type, drug efficacy, and survival. We also analyzed hepatotoxicity and colitis by dividing patients into severe (grades 3 and 4) and mild (grades 1 and 2) groups.
Results: The study included 362 patients: 38 in the irAE group and 324 in the control group. Among the 38 irAEs, there were 22 cases of liver injury, 14 of colitis (one overlapping with cholangitis), 2 of cholangitis, and 1 of elevated pancreatic enzymes. Progressive disease was more common in the control group (p=0.00774), suggesting greater treatment efficacy in patients with irAEs.
Further analysis of the 22 liver injury cases revealed 7 in the severe group and 15 in the mild group. No significant differences were found in medication types or the number of days to onset (61 vs. 48 days). Most severe cases required steroid treatment, initiated within a week, and liver injury improved in all cases.
Among colitis patients, 4 were in the severe group and 10 were in the mild group. The time to onset was longer in the severe group (p=0.0282), and their recovery time was also longer (p=0.0337). This suggests longer treatment may lead to more severe colitis. One patient in the severe group developed sepsis and died.
Conclusion: While irAEs may indicate effective treatment, severe cases can be challenging to manage, necessitating detailed measures.
PP-01-205
Clinical Characteristics and Outcomes in Patients With Localized Gastric Langerhans Cell Histiocytosis: A Case Series
Tae-Se Kim, Youngeun Oh, Yang Won Min, Hyuk Lee, Byung-Hoon Min, Jun Haeng Lee, Poong-Lyul Rhee and Jae J. Kim
Samsung Medical Center, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Localized gastric Langerhans cell histiocytosis (LCH) characterized by abnormal proliferation of Langerhans cells in the stomach without systemic involvement, is rare; therefore, the clinical characteristics and outcomes of LCH remain unclear. We investigated the clinical characteristics and outcomes in patients diagnosed with localized gastric LCH and have also discussed treatment strategies for this rare disease.
Materials and Methods: The study included seven patients diagnosed with localized gastric LCH at our hospital between September 1997 and December 2023. We retrospectively reviewed medical records and analyzed the clinicopathological characteristics and patient outcomes.
Results: Endoscopically, localized gastric LCH appeared as a small erosion in the distal part of the stomach. Positron emission tomography-computed tomography revealed normal findings in 100.0% (4/4) of patients during pre-treatment workup. Immunohistochemical analysis using S-100 and CD1a showed immunopositive cells in all tested patients. Of the six patients who underwent follow-up, two (33.3%) showed metachronous recurrence at a location distinct from the initial site. However, all patients eventually showed spontaneous regression of the disease, and no gastric LCH-induced mortality was observed during follow-up.
Conclusion: Careful and regular surveillance may be sufficient for patients with localized gastric LCH without systemic involvement.
PP-01-206
Endoscopic Features of Duodenal Epithelial Tumors and Histopathologic Diagnostic Capability by Immunostaining
Takuya Ohtsu1, Yu Takahashi1, Naohiro Nakamura1, Takuya Shijimaya1, Sanshiro Kobayashi1, Tomomitsu Tahara1, Koji Tsuta2 and Makoto Naganuma1
1Kansai Medical University Gastroenterology and Hepatology, Osaka, Japan;2Kansai Medical University Pathology and Division of Diagnostic Pathology, Osaka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The incidence of duodenal tumors is increasing partly due to improved endoscopic detection. However, preoperative diagnosis and treatment remain challenging. This study retrospectively analyzed endoscopically resected duodenal tumors, classified them based on mucosal characteristics, and evaluated marker expression for diagnostic improvement.
Materials and Methods: We reviewed endoscopic and immunostaining findings of duodenal epithelial tumors treated at our hospital from January 7, 2006, to May 15, 2020. Location, macroscopic features, and presence of accompanying lesions were analyzed using esophagogastroduodenoscopy. Immunostaining with MUC2, MUC5AC, MUC6, and CD10 classified each mucous trait, and Ki-67 expression was examined to determine their role in distinguishing malignancy.
Results: Fifty cases were examined. Endoscopically, a higher percentage of carcinomas were found in red lesions and duodenal bulb lesions. Immunostaining classified mucous traits into gastric, intestinal, and mixed gastrointestinal types, with 7, 23, and 20 cases, respectively. Adenocarcinoma was found in 4 (57.1%), 2 (8.7%), and 12 (60.0%) cases. Ki-67 expression was Adenocarcinoma 50%/NUMP 66.7% in gastric type, Adenocarcinoma 50%/Adenoma 71.4% in intestinal type, and Adenocarcinoma 41.7%/Adenoma 62.5% in mixed gastrointestinal type.
Conclusion: Duodenal tumors exhibit various mucous traits, complicating diagnosis and treatment. Endoscopic findings such as location in the bulb and a red appearance were significant, with a higher percentage of malignancy in gastric-type tumors. Measuring malignancy using Ki-67 in immunostaining proved to be difficult.
PP-01-207
Combining Endoscopic Submucosal Dissection and Chemoradiotherapy for Management of Rectal Cancer with Deep Submucosal Invasion
Jihye Park, Jae Hyun Kim, Sung Hyun Ko and Pf Seun Ja Park
Kosin University College of Medicine, Busan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Rectal cancer is one of the most prevalent malignancies worldwide. The introduction of Endoscopic Submucosal Dissection (ESD) offers a minimally invasive management option for early colorectal cancers. Intestinal resection after ESD is recommended in high risk groups with LNM, and chemoradiotherapy (CRT) is also effective adjuvant therapy. Herein, we report cases of patients who underwent ESD for rectal cancer and subsequently received concurrent CRT due to pathologically confirmed deep submucosal invasion.
Case Description: Three patients came to our hospital with LST lesions located near the anal verge. The tumors were excised using ESD, and histopathological examination revealed deep submucosal invasion (depths of 1,500 to 3,900 micrometers). Two patients showed moderate tumor budding, while the third patient had no tumor budding in histopathological examination. None of the three had lymphatic, venous, or perineural invasion. Due to the deep submucosal invasion and tumor budding, further treatment was necessary, but the patients declined surgery due to concerns about anal preservation. Therefore, chemoradiotherapy for two patients and radiotherapy for third patient was administered, and follow-up CT scans and colonoscopies have shown no evidence of recurrence to date.
Discussion: All three cases showed no evidence of recurrence on follow-up, indicating the effectiveness of combined treatment approach. This supports the potential role of CRT as adjunct to ESD in managing rectal cancer with high-risk features.
In conclusion, ESD followed by CRT or radiotherapy is a feasible and effective treatment strategy for rectal cancer with deep submucosal invasion, providing favorable oncological outcomes while preserving anal function.
PP-01-208
Age and Sex Differences in the Relationship of Body Weight Changes with Colon Cancer Risks
Jihye Park1, Pf Jae Hyun Kim1, Pf Young Ko2, Pf Hyung Jung Kim3 and Pf Seun Ja Park1
1Kosin University College of Medicine, Busan, South Korea;2Graduate School of Public Health, Korea University College of Medicine, Seoul, South Korea;3Department of Preventive Medicine, Korea University College of Medicine, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colon cancer is a significant health concern, and obesity is a well-established risk factor. This study investigated the association between body weight changes over 10 years and the incidence of colon cancer.
Materials and Methods: We included a healthy population that underwent a health checkup between January 2004 and December 2006 using the South Korean population database provided by the National Health Insurance Service (NHIS). We monitored them until August 2019 to identify newly developed cases of colon cancer. We categorized participants based on body weight changes and BMI. The primary outcome was the incidence of newly diagnosed colon cancer.
Results: There was no association with body weight changes in females < 40 years, while males who exhibited a weight gain > 20% of their body weight had a higher risk for colon cancer (HR 1.65, P>z <0.001 [95% CI 1.18–2.30]). For individuals ≥ 40 years of age, males who exhibited a body weight increase of 5%–20% had an increased risk for colon cancer (HR 1.12, P>z 0.00 [95% CI 1.06–1.19]). In females, a body weight decrease > 20% was associated with a decreased risk for colon cancer (HR 0.76; P> 0.02 [95% CI 0.60–0.96]) compared to the reference group with minimal body weight change (< 5%).
Conclusion: Our findings provide evidence that body weight changes are associated with either an increased or decreased risk for colon cancer. These findings emphasize the importance of considering body weight changes over time and tailoring prevention strategies based on age and sex.
PP-01-209
Comparison of the clinical profile of patients with Gallbladder cancer with and without Obstructive jaundice
1Dept. of Digestive Diseases and Clinical Nutrition, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India;2Clinical Research Secretariat and DAE-CTC, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India;3Dept. of Surgical Oncology, Tata Memorial Hospital, Homi Bhabha National Institute (HBNI), Parel, Mumbai, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Patients with gallbladder cancer (GBCA) frequently present with obstructive jaundice (OJ) which is associated with advanced disease and poor outcomes in retrospective studies. We compared the clinical features of GBCA patients with and without OJ at a high-volume referral cancer center.
Materials and Methods: 405 consecutive untreated patients with GBCA accrued over 2 years on a prospective IRB-approved study were analysed.
Results: 37% patients with GBCA (150/405) had jaundice at presentation with biliary obstruction on imaging qualifying as OJ. The mean age was 53 years (range 24-78). Patients presenting with OJ had a higher proportion of males as compared to without OJ (45% vs. 34%, p=0.025). There was no difference in the age at presentation or socioeconomic status between those with and without OJ. A significantly higher proportion of patients with OJ had a suboptimal ECOG PS of ≥2 (28% vs. 17%, p=0.010). They also had a significantly higher proportion of anorexia (53% vs. 38%, p=0.005), weight loss (35% vs. 22%, p=0.003) and malnutrition [SGA B/C: 86% vs 57%, p=0.000, PNI <45: 98 vs. 86%, p=0.001 and serum albumin levels ≤ 3.5 g/dL: 67% vs. 20%, p=0.000] compared to those without OJ. Patients with OJ were more likely to have inoperable disease (93% vs 82%, p=0.005) and CA 19-9 > 1000 U/mL (43% vs. 26%, p=0.001) than those without.
Conclusions: 37% of GBCA have OJ at presentation. Patients with OJ are more likely to have advanced disease and malnutrition. Survival analysis will help us rationalize treatment for these patients.
PP-01-210
Olfactomedin 2 Promotes the Growth and Metastasis of Colorectal Cancer
Hong Peng and Jin-Jun Guo
Bishan Hospital of Chongqing Medical University, Chongqing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colorectal cancer (CRC) is a common malignant disease worldwide. Liver metastasis affects the survival rate of CRC patients. Understanding the mechanism governing CRC progression is thus important. Olfactomedin 2 (OLFM2) is a secretory glycoprotein belonging to the OLFM family. Although several OLFM family members show the ability to regulate CRC progression, the role of OLFM2 in CRC is still unclear.
Materials and Methods: The expression of OLFM2 in 24 pairs of CRC and adjacent normal tissues was investigated using immunohistochemical analysis. The prognostic value of OLFM2 in CRC was studied based on publicly available data. Both in vitro and in vivo functional experiments were conducted to examine the effect of OLFM2 on CRC cell proliferation, migration, tumorigenesis, and metastasis.
Results: Compared with relative normal tissues, CRC tissues expressed significantly greater levels of OLFM2. Increased OLFM2 expression was associated with reduced survival of CRC patients. Multivariate cox regression analysis revealed OLFM2 expression as an independent prognostic factor in CRC. Knockdown of OLFM2 inhibited the proliferation and migration of CRC cells in vitro and impaired tumorigenesis and metastasis of CRC cells in vivo. Overexpression of OLFM2 promoted an aggressive phenotype in CRC cells.
PP-01-211
Mechanism of GAMT (Guanidinoacetate N-Methyltransferase) Promoting Liver Metastasis in Colorectal Cancer
Hong Peng and Jin-Jun Guo
Bishan hospital of Chongqing medical university, Chongqing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Liver metastasis is the most critical factor affecting the prognosis of colorectal cancer (CRC). In this study, we aimed to explored the mechanism of GAMT (Guanidinoacetate N-Methyltransferase) in liver metastasis of CRC.
Materials and Methods: Expression differences and prognosis of GAMT in CRC patients were analysed in GSE49355, TCGA, and HPA databases, which were validated by immunohistochemistry. Transwell, wound healing and stem cell sphere formation assays were conducted to investigate the impact of GAMT on cell migration, invasion and stemness. A nude mouse liver metastasis model (caecum and spleen) was established to explore the effect of GAMT on CRC liver metastasis. RNA sequencing (RNAseq) was performed to explore GAMT-related mechanisms.
Results: In GSE49355, TCGA and HPA database, upregulated GAMT was found in liver metastasis tissues comparing primary CRC, and showed a bad prognosis. Immunohistochemistry of the CRC liver metastasis tissue microarray found a similar results. In cell and nude mouse model, overexpression of GAMT enhanced the migration, invasion capabilities, stemness and liver metastasis of CRC cells, while knockdown of GAMT weakened these properties. RNAseq and western blot suggested overexpression of GAMT significantly increased the expression of C-myc and β-catenin, while knockdown of GAMT markedly decreased their expression. Wnt signaling pathway inhibitor DKK1 and β-catenin knockdown restored the effects of GAMT overexpression on cell migration and invasion capabilities in CRC cells.
Conclusion: GAMT enhances the migratory, invasive capabilities and stemness of CRC cells by activating the Wnt/β-catenin signaling axis, and promotes liver metastasis of CRC.
PP-01-212
Gastric cancer chemotherapy in the very elderly (Case Series)
Masaya Sano, Takashi Ueda, Hirohiko Sato, Erika Teramura, Mia Fujisawa, Ryouzou Deguchi, Masashi Matsushima and Hidekazu Suzuki
Tokai University School of Medicine, Isehara-city, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Reports on chemotherapy for gastric cancer in patients over 85 years old are limited. We aim to compile and report on cases of gastric cancer chemotherapy in patients over 85 years old experienced at our hospital.
Methods: From January 1, 2015, to December 31, 2023, we extracted data on patients over 85 years old who were newly registered with gastric cancer in the electronic medical records and underwent chemotherapy.
Results: Out of 2,218 gastric cancer patients, 141 were over 85 years old. Among them, 93 were men (66%). There were 22 surgical cases, 41 ESD cases, 3 cases of radiation therapy for bleeding control, and 10 cases of chemotherapy. 65 patients were under observation without treatment. All 10 chemotherapy cases were treated with S1 monotherapy. While it was difficult to follow up on the prognosis of all patients, we were able to track the outcomes of the 10 chemotherapy cases. No deaths were found due to serious adverse events caused by chemotherapy. However, this analysis did not demonstrate an improvement in prognosis.
Conclusion: Depending on the strong will of the patient and family, as well as the performance status and decision-making ability, S1 monotherapy might be considered as a treatment option for very elderly gastric cancer patients over 85 years old.
PP-01-213
Dietary Palmitic Acid Promotes The Proliferation And Migration Of Esophageal Cancer Through Flot2
Xiao Shi1,3, Chen Zhang2 and Ruihua Shi1,3
1Southeast university, Nanjing, China;2Second Affiliated Hospital, Nanjing Medical University, Nanjing, China;3Zhongda Hospital Southeast University, Nanjing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the mechanism of dietary palmitic acid (PA) on the proliferation and migration of esophageal cancer(EC).
Materials and Methods: After the esophageal cancer cells were treated with PA for 24 hours, all the protein samples were taken for high-throughput mass spectrometry(HTMS). And then the tissue sections of patients with EC were stained by immunohistochemistry. We set up normal EC cell group, PA treatment group, and FLOT2 deficient expression cells prepared by constructing FLOT2 small interference RNA to deal with PA. The effects of PA treatment and FLOT2 deficiency on cell proliferation, cell migration and activation of downstream signal pathway were verified in vitro, and subcutaneous tumor formation in nude mice was carried out.
Results: HTMS analysis showed that FLOT2 was up-regulated in EC. Bioinformatics analysis showed that FLOT2 expression in esophageal cancer tissues was significantly higher than that in normal tissues, and the diagnostic ROC curve showed that FLOT2 could differentiate EC to some extent. There were significant differences in FLOT2 expression of age and BMI in patients with EC. Compared with the control group, the expression levels of FLOT2, PI3K and p-AKT/AKT in PA treated group were significantly up-regulated.
Conclusion: The high FLOT2 expression in esophageal cancer comes from the fatty acid uptake of esophageal cancer cells. The activation of PI3K/AKT signal pathway promotes the proliferation and migration of esophageal cancer cells. Interfering with FLOT2 expression can reverse this tumor promotion phenomenon.
PP-01-214
A retrospective study of treatment outcomes and safety for Well-differentiated grade 3 pancreatic neuroendocrine tumors
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Well-differentiated grade 3 pancreatic neuroendocrine tumor (Pan-NET G3) is clinical presentation and the efficacy of therapy remain unclear. We therefore validated the clinical pathophysiology and prognosis of Pan-NET G3 in our institution.
Materials and Methods: We retrospectively examined the clinical characteristics, treatment courses, and prognoses of patients with unresectable Pan-NET at our department between June 2017 and April 2024.
Results: A total of 15 patients were identified. The median age was 69 (range: 35-75) years old, and 9 (60%) were men. The primary tumor site was the pancreatic head in 5 (33.3%), body/tail in 8 (53.3%), and postoperative recurrences in 2 (13.3%). Two patients had locally advanced disease, and 13 patients exhibited metastatic involvement. The metastatic sites included hepatic metastases in 12 (92.3%) patients, osseous metastasis in 1 (7.7%), and distant lymph node metastasis in 1 (7.7%). The histological diagnosis was G1/G2/G3/NEC/MINEN in 1/4/5/4/1 cases, respectively. The median Ki-67 labeling index was G1/2/G3/NEC in 2/10/40/75 patients, respectively. Fourteen patients underwent palliative first-line systemic therapy. The first-line systemic therapy was SSA in 1 patient, STZ-5FU in 8, mTOR in 1, cisplatin+etoposide in 2, irinotecan+etoposide in 1, and GEM+nab-PTX in 1. All G3 patients received STZ+5-FU therapy. The median overall survival was 23.2 (95% confidence interval [CI]: 20.0-73.4) months in G1/2, 4.8 (95% CI: 1.6-62.4) months in G3, and 5.5 (95% CI: 1.8-37.4) months in NEC.
Conclusion: Pan-NET G3 had a poorer prognosis than NEC. Further validation in larger populations is required; however, more therapeutic options are needed.
PP-01-215
Significance of psoas muscle index in patients with advanced cholangiocarcinoma undergoing palliative chemotherapy
Suk Pyo Shin
Cha Bundang Medical Center, Seongnam, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: We aimed to determiune wheter sacropenia affects on the prognosis in patients with advanced cholangiocarcinoma undergoing palliative chemotherapy.
Materials and methods: This study retrospectively reviewed the clinical outcomes of advanced cholangiocarcinoma patients receiving palliative chemotherapy between 2010 and 2022 that had adequate CT images. The cross sectional area of psoas muscle at L3 level was analyzed with CoreSlicer. Of the 93 patients reviewed, 47 patients that received gemcitabine, cisplatin, and Nab-paclitaxel were selected for this study.
Primary endpoint of this study was to evaluate the prognosis of patients with sarcopenia and secondary endpoint was to determine whether average PMI loss (since the first chemotherapy) during 6 month interval had any survival benefit.
Results: The cut-off value of PMI (psoas muscle index) for sarcopenia was set to 850mm2/m2 for males and 600 for females. The study comprised 24 males and 23 females, with a median age of 66.5 years.
10 of the male patients were sarcopenic and 14 females were sarcopenic. Average PMI loss was significantly greater in non-sarcopenic patients (18.9% in males and 7.6% in females, p<0.001) than sarcopenic patients. However, non-sarcopenic patients had no significant survival benefit compared to sarcopenic patients (p=0.270 in males, p=0.665 in females). Moreover, extent of PMI loss did not seem to have significant impact on survival (p=0.270 in males, p=0.665 in females)
Conclusion: Whether the patient had sarcopenia, as well as the average extent of PMI loss had no significant impact on overall survival of advanced cholangiocarcinoma patients undergoing palliative chemotherapy.
PP-01-216
Anthropometric Factors Influencing Accuracy of Preoperatory Staging in Colorectal Cancer Patients
Marsela Sina, Xhensila Pemaj, Dorina Osmanaj and Skerdi Prifti
University Clinic Of Gastrohepatology, Dept. of Internal Disease, University of Medicine, Tirana, Albania
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Accuracy of preoperative staging for colorectal cancer (CRC), may be prone to patients’ individual characteristics. We aim to identify specific factors influencing the accuracy of preoperative TNM-staging by Computed Tomography (CT).
Methods: Our single-center study included patients who underwent surgery for CRC, at the University Hospital Center Mother Teresa, during 2014-2017. Demographics, body measurement: body mass index (BMI), waist circumference, hips circumference, waist-to-hip ratio; and preoperative and postoperative pathologic stages, were collected for each patient. Patients were divided into accurate and mis-staging groups according to the comparison between preoperative and postoperative TNM.
Results: 262 CRC patients (123 rectum, 139 colon), mean age 62.7 ± 11.0 years, 56.1% male; 43.9% female. Mean BMI 26.2±2.98 kg/m2; mean waist circumference 94.2±12.2cm; mean hip circumference 106.9±11.4cm and mean waist-to-hip ratio 0.9±0.1. According to WHO, 1.5% of patients were classified as underweight, 29.2% as normal weight; 58.3% as overweight, and 11% obese. 47% of patients were mis-staged of which 70.3% under-staged and 29.7% over-staged.
No significant association was found between gender (p=0.671), BMI (p=0.561), waist circumference (p = 0.299), hip circumference (p = 0.560), waist-to-hip ratio (p = 0.957), and the accurate staging.
Individuals > 50 years were significantly more likely to be mis-staged compared to under 50 (χ² = 4.488, p = 0.034).
Conclusions: In our cohort, preoperative mis-staging occurred in 47% of cases, predominantly as under-staging. Statistical analysis revealed no association between mis-staging and BMI, waist circumference, hip circumference, and waist-to-hip ratio. Age over 50 emerged as significant predictor for CT mis-staging.
PP-01-217
Sex-specific roles of NRF2 and PD-L1 in right-sided colorectal cancer development: Molecular insights and implications
Chin-hee Song1, Nayoung Kim1,2, Yonghoon Choi1, Ryoung Hee Nam1, Soo In Choi1, Jae Young Jang1, Eun Hye Kim1, Sungchan Ha1 and Ha-Na Lee3
1Departments of Internal Medicine and Research Center for Sex- and Gender-Specific Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, South Korea;2Department of Internal Medicine and Liver Research institute, Seoul National University College of Medicine, South Korea;3College of Pharmacy, Seoul National University, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study examined the roles of nuclear factor erythroid 2-related factor 2 (NRF2) and programmed death ligand 1 (PD-L1) in colon carcinogenesis, underscoring on sex and tumor location.
Materials and Methods: A total of 378 participants were enrolled from Seoul National University Bundang Hospital: 88 healthy controls (HC), 139 patients with colorectal adenoma (AD), and 151 patients with colorectal cancer (CRC). During colonoscopy, the tissue samples were collected. Quantitative real-time PCR, methylation-specific PCR, and immunohistochemistry (IHC) were performed utilizing tumor samples from patients and normal mucosa in the HC group.
Results: NRF2 mRNA expression was higher in the CRC group than in the HC and AD groups, with decreased NRF2 methylation in the AD and CRC groups. NRF2 protein expression, as evaluated by IHC, increased in the AD and CRC groups relative to that in the HC group. PD-L1 protein expression was remarkably higher in the CRC group than in the HC and AD groups. These patterns were consistent in both males and females. In sex- and CRC location-specific analyses, NRF2 protein expression was significantly higher in females, particularly in patients with right-sided CRC. Moreover, females exhibited increased PD-L1 mRNA expression compared to males in the AD group, and PD-L1 mRNA levels were higher in females with right-sided colon cancer than in those with cancer at other locations.
Conclusion: Differences in NRF2 and PD-L1 expression indicate site-specific colon carcinogenesis based on sex, particularly in females with right-sided CRC.
PP-01-218
Primary Extra-Gastrointestinal Stromal Tumour (EGIST) of the retro-gastric mesentery: A case report
Duminda Subasinghe1, Harry Prashath2, Chamod Nirmal2, Supun Kulathunga4 and Harshima Wijesinghe3
1Division of HPB/GI Surgery, Department Of Surgery, university Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital Of Sri Lanka, Colombo, Sri Lanka;3Department of Pathology, University of Colombo, Colombo, Sri Lanka;4Department of Gastroenterology, The National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal stromal tumors (GISTs) are rare, accounting for only 0.1–3% of gastrointestinal malignancies, while extragastrointestinal stromal tumors (EGISTs) constitute 5% of all GISTs. Predominantly originating from Cajal-like cells, EGISTs primarily occur in the omentum, mesentery, retroperitoneum and rarely in pancreas, liver, gallbladder, urinary bladder, pleura, prostate, seminal vesicles, pelvis and vagina presenting unique diagnostic challenges. Unlike GIST they can metastasize to lymphnodes. Herein, we report a case of primary retro-gastric mesentery EGIST.
Case Description: A 48-year-old male incidentally discovered a large intra-abdominal mass during an ultrasound scan for left sided ureteric colic. CECT revealed a large irregular vascular tumour measuring 12 x 15 x 20 cm posterior to the stomach. Intraoperatively, a tumour adherent to the stomach's posterior wall and transverse mesocolon was completely resected. He had an uneventful postoperative recovery. Histopathological examination confirmed EGIST of spindle cell type with an intermediate risk, risk stratification (AFIP) group 3b with a 12% disease progression risk.
Discussion: EGISTs often present insidiously or with compressive symptoms, necessitating thorough diagnostic workup. While imaging aids in localization, definitive diagnosis hinges on histopathological assessment, particularly immunohistochemistry for CD117. Management revolves around surgical excision. The utility of imatinib mesylate remains uncertain. EGISTs pose diagnostic and therapeutic challenges, with surgery being the primary treatment modality. Optimal management strategies, including the role of adjuvant therapy with KIT inhibitors, require further investigation to improve outcomes and prognostication.
PP-01-219
Ectopic Pancreas Mimicking Gastrointestinal Stromal Tumor (GIST): A Case Report
Dedy Sudrajat2, Stefanus Ranty2, Jeffrey Jeffrey2 and Riesye Arisanty2
1Eka Hospital Bsd, Jakarta, Indonesia;2Grha Kedoya Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal stromal tumor (GIST) remains the most common submucosal tumor in stomach. This tumor is found mostly in gastric body. The main challenge in diagnosis of GIST remains in distinguishing it from benign lesions that do not require resection. Here we present ectopic pancreas, a submucosal tumor, mimicking as GIST lesion.
Case Description: A 31-year-old female patient was referred with chief complaint epigastric patient and nausea for a week. No loss of appetite, vomiting, heartburn, bleeding, nor loss of weight were reported. Vital sign and laboratory test results were all within normal limit. Physical examination showed pain palpated in epigastric area. Esophagogastroduodenoscopy (EGD) was performed and found tumor located in anterior wall of greater curvature of corpus, approximately 1.5 cm in diameter. Biopsy was taken, and abdominal Computed Tomography (CT) scan showed mass of gastric wall with the size of 1.3x1.7x1.7 cm in anterior wall of greater curvature of corpus, neither lymph node or any adjacent organs were involved. Patient was then undergone laparoscopic resection, and histopathology results showed ectopic pancreas. Patient was discharged 3 days after surgery with no complaint.
PP-01-221
A 3-weekly TS-1 regimen as adjuvant therapy for stage II and III advanced gastric cancer
Sun Jin Sym1, Jihong Bae1, Dong Bok Shin1, Min-Hee Ryu2, Porf Dae Young Zang3 and Woon Kee Lee4
1Gachon University Gil Medical Center, Medical Oncology, Incheon, Republic of Korea Incheon, South Korea;2Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea;3Division of Hematology-Oncology, Department of Internal Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea;4Surgery, Gachon University Gil Medical Center, Incheon, Republic of Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: We investigated the efficacy and safety of a 3-weekly adjuvant TS-1 regimen for 1-year in advanced gastric Cancer (AGC).
Materials and Methods: We analyzed 93 stage II, III AGC patients who initiated a 3-weekly adjuvant TS-1 therapy from Feb 2017 to May 2022 after gastrectomy with D2 lymphadenectomy. A 3-weekly regimen consisted of TS-1 at a dosage of 80 mg/m2/day, for 2 weeks, followed by a 1-week rest until reaching a 1-year or 16 course.
Results: In the total 93 patients, 12 patients (13%) have experienced disease recurrence during the follow-up period (median 24.6 months, range 4.2 to 63.3). We observed that 7 cases(9.5%) among the Stage II patients (n=73) were evaluated as recurrent disease, while 5 cases (25%) in the Stage III patient group(n=20) were assessed as recurrence. In Kaplan-Meier analysis, the Recurrence-Free Survival(RFS) rates at 1 year, 3 years, and 5 years for the entire patients were estimated as 92.0% (95% CI 86.5 to 97.9), 84.7% (95% CI,76.4 to 93.9), and 78.6% (95% CI, 65.8 to 94.0), respectively. 80 patients (86%) completed adjuvant 3-weekly TS-1 treatment for 1-year or 16 courses. 25 patients (26.9%) completed the treatment but required dose reduction during the entire course of therapy. Adverse events, mainly diarrhea (28%) and nausea (20%), were primarily of Grade 1 or 2.
Conclusion: 3-weekly TS-1 regimen as adjuvant therapy exhibited good efficacy, manageable toxicity. This 3-weekly regimen as adjuvant therapy in AGC should be evaluated in future study.
PP-01-222
Gastric MALT Lymphoma with Disseminated Disease to Rectum: A Rare Entity
Erika Johanna Tañada-Escanlar and Melchor Chan
UST Hospital, manila, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Mucosa-associated lymphoid tissue (MALT) lymphomas usually are confined to single extranodal organs commonly the stomach . We present a rare case of disseminated MALT lymphoma affecting the rectum and stomach presenting as painless hematochezia and rectal prolapse.
Case Presentation: A 69-year-old Filipino male, hypertensive and diabetic complained of painless hematochezia with nontender manually reducible rectal mass. Digital rectal examination revealed a soft palpable circumferential mass. Colonoscopy exhibited multiple thickened edematous folds from 10 cm of the anal verge to the rectum. Endosonography showed a circumferential wall thickening involving all the layers of the colonic and rectal wall with perirectal lymphadenopathy. Whole Abdomen CT scan exposed a large enhancing lobulated eccentric wall thickening at the rectal region with thickened perirectal fascia with subcentimeter perirectal lymph nodes and irregularly thickened gastroesophageal junction. EGD showed a 2 cm ulcerating lesion, raised with irregular nodular border in the cardia of the stomach and negative for Helicobacter pylori infection. Histopathology from both rectal and gastric cardia revealed atypical lymphoid proliferation and Immunohistochemical Staining was positive for CD3, CD20, BCL2 and Ki-67, confirming the diagnosis of MALT Lymphoma. A multidisciplinary team was called and patient was started on Bendamustine plus Rituximab chemotherapy protocol.
Conclusion: Notwithstanding the fact that some case reports have been published, clinicopathologic characteristics of multiorgan MALT lymphomas remain unclear. This case report highlights the importance of recognizing this rare multi-organ disease that can help elucidate optimal treatment plan.
Keywords: case report; Disseminated MALT lymphoma, Rectal Lymphoma
PP-01-223
PATIENTS CHARACTERISTICS AND THE CORRELATION BETWEEN BMI TO HISTOLOGICAL GRADING OF ESOPHAGEAL CANCER
Endro Tanoyo
Sardjito Hospital, PPHI-PGI-PEGI, Sleman, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate patients characteristics, and correlation between body mass index (BMI) to histological grading of esophageal cancer in Yogyakarta population.
Methods: Outpatients data were collected from electronic medical record of Sardjito Hospital in Yogyakarta, Indonesia, from Mei 1st 2019 to Mei 31st 2024. The study included patients over 18 years old who had proven as esophageal cancer with completed histological grading. There were 41 subjects divided into four groups based on WHO BMI categories and the histological grading divided into five criteria (well, well to moderate, moderate, moderate to poor, and poor differentiated). The data was analyzed using descriptive statistics, and a chi-square crosstabulation.
Results: 41 patients were proven as adenocarcinoma and squamous cell carcinoma; 58,5% patients were underweight, while overweight and obese at 4,9% and 2,4%, respectively. Most of the patients were young and male at equal 65,9%. Overall percentage of Squamous cell carcinoma was 61%. In underweight group the percentage of adenocarcinoma and squamous cell carcinoma were equal (50%). The percentage of moderately differentiated in the group of underweight, overweight and obese were 37,5%, 50% and 100% respectively. The difference in histological grading between the four BMI groups was not significantly different (p = 0.862).
Conclusions: Although there were no statistically significant differences of BMI to histological grading between the four groups, the most various histological grading were found in underweight group. Squamous cell carcinoma is the most common type in all groups and the majority are male. However, adenocarcinoma was found in 50% of underweight group.
PP-01-224
Clinicopathological features and prognosis of metastatic tumors in the small bowel: A large multicenter analysis
1Department of Gastroenterology, Hiroshima University Hospital, Japan;2Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Nippon Medical School, Japan;3Department of Gastroenterology, Nippon Medical School, Graduate School of Medicine, Japan;4Department of Medicine, Division of Gastroenterology, Jichi Medical University, Japan;5Department of Colorectal Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Japan;6Division of Colon and Rectal Surgery, Shizuoka Cancer Center, Japan;7Division of Endoscopy, Shizuoka Cancer Center, Japan;8Department of Gastrointestinal Oncology, Osaka International Cancer Institute, Japan;9Department of Gastroenterology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Japan;10Digestive Disease Center, Showa University Northern Yokohama Hospital, Japan;11Department of Lower Gastrointestinal Medicine, Cancer Institute Hospital of the Japanese Foundation for Cancer Research, Japan;12Department of Gastroenterological Surgery, Toranomon Hospital, Japan;13Department of Surgery, Kyorin University School of Medicine, Japan;14Division of Lower Gastrointestinal Surgery, Department of Surgery, Hyogo Medical University, Japan;15Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Japan;16Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Japan;17Department of Gastrointestinal Surgery, Tokyo Medical and Dental University, Japan;18Division of Gastroenterology, Department of Internal Medicine, Iwate Medical University, Japan;19Department of Diagnostic Pathology, Iwate Medical University, Japan;20Department of Gastroenterology and Hepatology, Gunma University Graduate School of Medicine, Japan;21Division of Gastroenterology, Matsuyama Red Cross Hospital, Japan;22Department of Gastroenterological Surgery, Saitama Medical University International Medical Center, Japan;23Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Japan;24Department of Surgery, Hiroshima City Hiroshima Citizens Hospital, Japan;25Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, Japan;26Department of Surgery, Kurume University School of Medicine, Japan;27Department of Gastroenterology and Endoscopy, National Cancer Center Hospital East, Japan;28Department of Colorectal Surgery, National Cancer Center Hospital East, Japan;29Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Saga University, Japan;30Department of Coloproctological Surgery, Faculty of Medicine, Juntendo University, Japan;31Department of Human Pathology, Juntendo University Graduate School of Medicine, Japan;32Department of Gastrointestinal Surgery, Kansai Medical University Hospital, Japan;33Department of Clinical Oncology, Kyoto University Hospital, Japan;34Department of Surgery, Teikyo University Chiba Medical Center, Japan;35Department of Gastroenterology, Maebashi Red Cross Hospital, Japan;36Department of Gastroenterological Surgery, Aichi Cancer Center Hospital, Japan;37Department of Gastroenterology, Asahikawa City Hospital, Japan;38Department of Colorectal Surgery, National Cancer Center Hospital, Japan;39Endoscopy Division, National Cancer Center Hospital, Japan;40Department of Surgery, Coloproctology Center Takano Hospital, Japan;41Department of Surgical Oncology, The University of Tokyo, Japan;42Department of Gastroenterology and Oncology, Tokushima University Graduate School of Biomedical Sciences, Japan;43Department of Coloproctology, Aizu Medical Center Fukushima Medical University, Japan;44Department of Surgery, National Defense Medical College, Japan;45Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Japan;46Department of Colorectal Surgery, Japanese Red Cross Medical Center, Japan;47Department of Advanced Endoscopy, Fujita Health University School of Medicine, Japan;48Department of Surgery, NHO, Osaka National Hospital, Japan;49Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Japan;50Department of Gastroenterology, Hiroshima City North Medical Center Asa Citizens Hospital, Japan;51Department of Surgery, Japanese Red Cross Omori Hospital, Japan;52Tokyo Medical and Dental University, Japan;53Division of Molecular and Diagnostic Pathology Niigata University Graduate School of Medical and Dental Sciences, Japan;54Department of Gastroenterology, JA Onomichi General Hospital, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Owing to the rarity of metastatic tumors in the small bowel, the current understanding of their clinicopathological features and prognostic factors is limited, and we aimed to clarify these aspects in our study.
Materials and Methods: We retrospectively examined 272 patients who were diagnosed histopathologically with metastatic tumors in the small bowel between May 2005 and August 2018 at multiple centers in Japan. We examined clinical features, such as age, sex, chief complaint, diagnosis modalities, primary site, treatment, and long-term prognosis.
Results: In this study cohort, obstructive symptoms occurred most frequently (45% abdominal pain, 22% vomiting), and overt or occult gastrointestinal bleeding was present in 21% of patients. Enteroscopy, balloon-assisted enteroscopy, and capsule endoscopy were performed in 33%, 29%, and 13% of patients, respectively. The most common primary tumor was lung cancer (36%), followed by colorectal cancer (17%), gastric cancer (9%), and malignant melanoma (6%). Surgery (including tumor resection or bypass surgery) was performed in 78% of patients. Among the patients, the 3-year and 5-year overall survival rates were 32% and 24%, respectively. Multivariate analysis showed that surgical treatment significantly affected overall survival (Hazard ratio: 0.63, 95% Confidence Interval: 0.40–0.99, p=0.04).
Conclusion: The lung was the most frequent primary site of metastatic tumors in the small bowel. Multivariate analysis showed that surgical treatment was a single factor that affected overall survival. Surgical treatment was associated with improved overall survival.
PP-01-225
A correlative analysis of molecular subtypes of gastric cancer in context to clinicopathological profiles
Kim Vaiphei
Punjab Institute Liver Biliary Sciences, Mohali, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: a comprehensive molecular analysis of gastric cancer (GC) correlating with the histological type and patient outcome.
Materials and methods: 100 biopsy of gastric cancer analysing clinical and pathological and were assessed for MMR protein, p53 and E-cadherin by IHC; Her2/neu was assessed by IHC and FISH.
Results: 57 - intestinal type and 43 diffuse type. Age - 25 to 90 years, mean -53.3. M:F=3.3:1. Diffuse type more common in younger female patients. 8 intestinal type were MMR deficient observed in older patients, distal tumor better outcome with wild type p53. Aberrant p53 expression in 51% associated with larger tumor and positive lymph node. E-cadherin was lost in 24 diffuse type. Her2/neu was overexpressed 15 intestinal type. Follow-up. Mean survival of patients with MMRd compared to MMR proficient were 44.8 and 22.9 months respectively. Her2/neu was associated with worst outcome. p53 and E-cadherin status did not influence patient outcome.
Conclusion: The study brings out the importance roles of MMR protein and Her2/neu in patient selection for targeted therapy.
PP-01-226
Prevotella copri Enhances Colorectal Carcinogenesis via Wnt Signaling Pathway Activation
Fucheng Wang
Department Of Gastroenterology And Hepatology, General Hospital, Tianjin Medical University, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to elucidate the role of Prevotella copri (P.copri), a gut microbe found in higher abundance in colorectal cancer (CRC) patients, in the development of CRC and its potential as a therapeutic target.
Materials and Methods: We utilized colonic epithelial cell lines Caco-2 and HT-29 to assess the influence of P.copri supernatant on cell proliferation, migration, and invasion. RNA-Seq was conducted on these cells to identify gene expression changes. In vivo effects were evaluated using Apc min/+ mice, with analyses of colon tissues and fecal samples through histopathology, qPCR, western blotting, and 16S rDNA sequencing.
Results: P.copri was confirmed to be more abundant in CRC patients by GMrepo database search and qPCR. Its supernatant increased CRC cell aggressiveness and activated the Wnt signaling pathway, as shown by RNA-Seq. Mice treated with P.copri developed more intestinal tumors, with increased tumor proliferation, reduced apoptosis, and impaired gut barrier function. The treatment also led to a shift in gut microbiota, increasing pathogenic bacteria and decreasing SCFA producers, affecting SCFA levels. Correlations between Butyricicoccus, Harryflintia, and differential gene expression were identified.
Conclusion: P.copri drives colorectal tumorigenesis through inducing gut barrier dysfunction, the Wnt pathway activation and gut microbial dysbiosis with SCFAs-producing bacteria decreased. This confirms the close relationship between gut microbiota dysbiosis and CRC, suggesting that novel strategies targeting the gut microbiota may be effective against CRC.
PP-01-227
Reduced intestinal-to-diffuse conversion and immunosuppressive responses underlie superiority of neoadjuvant immunochemotherapy in gastric adenocarcinoma
Lei Wang1, Linghong Wan1, Xu Chen1, Peng Gao2, Zhenning Wang2 and Bin Wang1
1Department of Gastroenterology & Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Chongqing, China;2Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, China Medical University, Shenyang, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Neoadjuvant immunochemotherapy (NAIC) may lead to significant pathologic regression in locally advanced gastric adenocarcinoma (GAC), whether NAIC achieves superior benefits over neoadjuvant chemotherapy (NAC) warrants further clinical study. It is also poorly understood how the malignant cells and tumor immune microenvironment (TIME) might respond differentially to NAIC and NAC that may underlies therapeutic efficacies.
Materials and Methods: This multi-center retrospective study included a total of 295 patients, with 67 patients receiving NAIC and 188 patients receiving NAC. Propensity score matching was utilized to analyze the neoadjuvant efficacy of the two treatment groups. Treatment-naive and paired tumor tissues after D2 gastrectomy were collected for pathological, immunological, and transcriptomic analysis.
Results: NAIC demonstrated significantly increased rate of pathologic complete response compared to NAC (pCR: 25% vs 4%, P<0.05). Interestingly, pretreatment intestinal subtype of Lauren's classification was predictive of pathologic regression following NAIC, but not NAC. A substantial portion of cancers underwent intestinal-to-diffuse transition, which occurred less following NAIC and correlated with treatment failure. Moreover, NAIC prevented reprogramming to an immunosuppressive TIME with less active fibroblasts and exhausted CD8+ T cells, and increased numbers of mature tertiary lymphoid structures. Mechanistically, activation of the TNFα/NF-κB signaling pathway was associated with response to NAIC.
Conclusion: NAIC is superior to NAC for locally advanced GAC, likely due to reduced intestinal-to-diffuse conversion and reprogramming to an immuno-active TIME. Modulation of the histological conversion and immunosuppressive TIME could be translatable approaches to improve neoadjuvant therapeutic efficacy.
PP-01-228
Intestinal-subtype as a biomarker predicting response to neoadjuvant immunochemotherapy in locally advanced gastric adenocarcinoma
Lei Wang, Mengting Sun, Jinyang Li, Linghong Wan and Bin Wang
Department of Gastroenterology & Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Neoadjuvant immunochemotherapy (NAIC) significantly improves pathologic regression in locally advanced gastric adenocarcinoma (GAC). However, there is still a lack of effective biomarkers to stratify the beneficiary population for NAIC.
Materials and Methods: This prospective, open-label, single-arm phase II clinical study enrolled patients to evaluate the efficacy and safety of NAIC for LAGC. To identify biomarkers for the efficacy of NAIC, we analyzed the correlation between clinical pathological characteristics and neoadjuvant efficacy, and used bulk RNA-seq of 66 paired samples before and after NAIC and scRNA-seq of 105 samples collected from 8 public databases for comprehensive analysis to explore the characteristics of the tumor immune microenvironment (TME) with different Lauren’s classifications and differences in NAIC response.
Results: The study achieved the pre-specified primary endpoint: pCR rate was 30%, MPR rate was 43%, and safety and feasibility were met. The analysis revealed that Lauren’s classification may be a key feature influencing the efficacy of immunotherapy in gastric cancer. Further transcriptomics analysis revealed that intestinal-type gastric cancer (IGC) has a higher proportion of DNA damage repair malignant cells and CLEC9A+ DCs, and these two key cell subsets are highly correlated with efficacy of immunotherapy for gastric cancer. Intestinal.sig, constructed based on Lauren’s classification, can accurately predict the efficacy of gastric cancer immunotherapy.
Conclusion: NAIC has good efficacy and feasibility for patients with LAGC, especially in patients with IGC. Intestinal.Sig is a novel biomarker for immunotherapy effect, which may help to discriminate patients with response to NAIC.
PP-01-229
Mechanism of Vitamin D Inhibiting the Development of Colon Tumors by Regulating MMP1
Lu Wang1, Xin Chen1 and RUI Ze Xu2
1Tianjin Medical University, Tianjin, China;2Baotou Medical University, Baotou, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To explore the relationship and mechanisms between vitamin D(VD) and colorectal tumors, offering insights into the diagnosis and treatment of colorectal cancer (CRC).
Materials and Methods: Serum levels of 1,25(OH)2D3 were measured using the double-antibody sandwich. Bioinformatics analysis identified VD-related colorectal tumor genes, validated using the HCT116. HUB genes' changes were analyzed via RT-qPCR and Western blot.
Results: 1. Serum levels of 1,25(OH)2D3 were 42.99±6.02μg/ml in the normal group, 37.06±9.56μg/ml in the CRA group, and 19.00±5.96μg/ml in the CRC group (p<0.05). No significant differences were found in VDR SNP among the groups (Figure 1).
2.VD-related colon cancer genes (Figure 2) exhibited significant expression differences among the groups. LASSO regression analysis identified 5 genes (Figure 3A-D). The diagnostic model constructed from these genes demonstrated high diagnostic efficiency (Figure 3E-L) and performed well in the TCGA-COAD dataset (Figure 4A-B).
3.RT-qPCR: SOSTDC1, PRKAA2, and CEACAM1 expressions decreased across the groups, while MMP1 and CCND1 expressions increased (Figure 4C).
4.In vitro experiments indicated that calcitriol inhibits the proliferation and migration of the HCT116(Figure 5), and significantly alter the expression of hub genes (Figure 6A). GSEA of MMP1 revealed a significant correlation with the JAK-STAT signaling pathway (Figure 6B). Calcitriol stimulation resulted in decreased expressions of MMP1, JAK, and STAT5 (Figure 6C).
Conclusion: The serum of VD is significantly lower in CRC. VD inhibits the proliferation and migration of colon cancer cells, reduces oncogene expression. Therefore, VD holds significant potential in the diagnosis and treatment of colon cancer.
PP-01-230
Identification of Extracellular Matrix-Related Genes in the Progression of Gastric Cancer with Intestinal Metaplasia
Lu Wang and Xin Chen
Tianjin Medical University, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: By analyzing transcriptome sequencing data, molecular biomarkers that can predict the risk of gastric cancer (GC) and monitor the progression of intestinal metaplasia (IM) can be identified, providing new insights and strategies to prevent the progression of IM to GC.
Materials and Methods: Weighted gene co-expression network analysis (WGCNA) was used to identify gene modules. Upregulated ECM-related genes were further examined using univariate Cox regression and Lasso analysis to select hub genes and construct a survival analysis model. The intestinal cell model was established by stimulating GES-1 cells with CDTA.
Results: 1. WGCNA identified 1709 DEGs from GSE191275(Figure 1), while TCGA-STAD identified 4633 DEGs (Figure 2A). The intersection revealed 71 upregulated and 171 downregulated genes enriched in ECM-related pathways (Figure 2B-D).
2. Univariate Cox regression identified 6 genes with prognostic significance (Figure 3). Lasso regression identified SPARC/SERPINE1 as non-zero coefficient genes (Figure 4A-B). A prognostic model integrating clinical TNM staging, age, SERPINE1, and SPARC was constructed (Figure 4C-G).
3. IHC confirmed increasing SPARC protein expression across normal gastric mucosa (-), intestinal metaplasia (+-~+), and gastric cancer (+~++), with significant differences (p < 0.05) (Figure 5A-C). WB analysis showed significantly higher SPARC expression in induced intestinal cells compared to GES-1(Figure 5D).
Conclusion: Comprehensive analysis suggests that a model incorporating clinical TNM staging, age, and SPARC/SERPINE1 expression serves as a prognostic predictor for GC. Moreover, elevated SPARC expression in IM and GC suggests its potential as a promising biomarker for early detection of GC and as a novel therapeutic target, guiding clinical applications.
PP-01-231
Midp619 mediates EMT to promote colorectal cancer progression by enhancing PKM2 stability
女士 Yali Wang, Weilong Zhong and Wentian Liu
Tianjin Medical University General Hospital, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Our previous study showed that MicroRNA-derived protein (MidP619) is overexpressed in colorectal cancer (CRC) and promotes malignant progression of CRC. The aim of this study was to investigate the specific mechanism by which MidP619 promotes the malignant progression of CRC.
Materials and Methods: MidP619 expression in human CRC and paracancerous tissues was detected using Western blotting (WB) and immunohistochemistry. The effect of MidP619 in proliferation, invasion, and migration was investigated using the CCK 8 assay, colony formation assay, transwell assay, wound healing assay, and animal investigations. RNA sequencing and WB were used to identify MidP619-regulated genes. Protein interactions with MidP619 were found using immunoprecipitation and mass spectrometry. PKM2 ubiquitination was established using cellular ubiquitination and protein stability tests. Finally, glycolysis-related indicators were assessed in CRC cells.
Results: MidP619 was substantially expressed in CRC cells, promoting their proliferation, invasion, and migration. RNA sequencing and western blot analysis revealed that MidP619-regulated genes were involved in EMT and metabolic processes. Co-IP and immunofluorescence tests revealed that MidP619 interacted with PKM 2, affecting protein levels. PKM2 protein levels were raised in OE-MidP619 CRC cells, and they were found to be positively linked with EMT promotion. Cellular ubiquitination and protein stability experiments confirmed that this relationship improves protein stability and increases glycolysis by decreasing PKM2 ubiquitination levels.
Conclusion: We demonstrated that MidP619/PKM2/glycolysis-induced EMT promotes CRC progression. This is the first work to reveal the functional and metabolism-related mechanisms of Midp619 in CRC, and lays the foundation for future diagnosis and targeted therapy of CRC.
PP-01-232
The characteristics of early gastric cancer immune microenvironment with different EBV and H.pylori infection status
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: To compare the difference of immune cells and characteristics of tertiary lymphoid structures (TLS) in the tumor microenvironment of early gastric cancer (EGC) with different Epstein-Barr virus (EBV) and Helicobacter pylori (H. pylori) infection status.
Materials and Methods: The clinicopathological data of 247 EGC patients in Peking University Third Hospital from 2010 to 2022 were collected. By the results of EBV-encoded small RNA(EBER) in situ hybridization and H. pylori immunohistochemical staining, patients were divided into EBV and H. pylori double-positive(EBV+HP+), EBV single-positive(EBV+HP-), H. pylori single-positive(EBV-HP+) and double-negative(EBV-HP-) group. The immune cell infiltration in the mucosa was compared. 41 patients were selected to detect the tumor-infiltrating immune cells and TLS by H&E and multiplex immunofluorescence staining.
Results: The proportion of background mucosa with massive lymphocyte infiltration in the EBV+HP- group was significantly higher than the other groups(18.2% vs 12.5% vs 0% vs 0%, p=0.015). The number of tumor-infiltrating T cells was significantly higher in the EBV+HP- group than EBV-HP+ and EBV-HP- group([1558(552.4, 2149)]vs. [440.5(353.3, 556.5)]vs. [368.7 (190.5, 543.8)]/mm², p<0.001). The density of intratumoral TLS was significantly higher in the EBV+HP- group than EBV-HP+ and EBV-HP- group [(0.5192±1.15)vs. (0.008±0.028)vs.(0.015±0.041)/mm², p=0.009]. The average distance between peritumoral TLS to the tumor was significantly greater in the EBV-HP+ group than EBV+HP- and EBV-HP- group([1.240(0.100, 4.400)]vs. [0.365(0.000, 2.145)]vs. [0.500(0.000, 1.750)]mm, p=0.008). Secondary follicle-TLS (SFL-TLS) was predominant in all groups.
Conclusion: The immune microenvironment of EGC is dissimilar under different EBV and H. pylori infection status, demonstrating the roles of pathogens in EGC.
PP-01-233
Identification of macrophage-related gene signatures to explore potential molecular mechanisms in intestinal metaplasia
教授 Huiling Yu1,2, Kui Jiang1 and Jingwen Zhao1
1Tianjin Medical University General Hospital, Tianjin, China;2Affiliated Hospital of Hebei University, Baoding, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Macrophages are participants in chronic inflammation during Gastric intestinal metaplasia (IM). The purpose of this study was to investigate macrophage-related genes (MRGs) signatures and molecular mechanisms in IM.
Methods: The GSE78523 dataset was used to acquire the differentially expressed genes (DEGs) between tissue samples of IM patients and normal individuals. Next, the intersection genes of DEGs and MRGs was regarded as differentially expressed MRGs (DE-MRGs). The least absolute shrinkage and selection operator and support vector machine recursive feature elimination were utilized to screen candidate genes in IM. Subsequently, the expression level and diagnostic effectiveness of the DE-MRGs were further validated via GSE60427, and nomogram was constructed based on these gene signatures. Meanwhile, enrichment analysis and immune cells infiltration analysis were accomplished. Finally, the regulatory mechanisms analysis and drug prediction of gene signatures were implemented.
Results: APOB, SIRPA, SUCNR1 and IFNGR1 were identified as hub DE-MRGs by expression level analysis. The nomogram model had a great prediction ability of IM occurrence. GSEA enrichment analysis suggested that the hedgehog signaling pathway, ascorbate, aldarate metabolism and pentose and glucuronate interconversions were primarily involved. Furthermore, immune analysis revealed hub DE-MRGs were markedly associated with central memory CD8+ T cell, gamma delta T cell, monocyte, etc. Moreover, the qRT-PCR findings revealed a significant increase for SIRPA and IFNGR1 expression in IM samples, but SUCNR1 expression trends in the opposite direction.
Conclusion: APOB, SIRPA, SUCNR1 and IFNGR1 were identified as hub DE-MRGs in IM, which provided some reference for further research IM in clinical practice.
PP-01-234
SP1-induced lncRNA ZFAS1 contributes to cell proliferation and migration in gastric cancer through AKT/mTOR signaling
Fan Zhang and Ying Li and Liang He and Yu Wang
First Hospital Of Jilin University, Changchun City, Jilin Province, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Gastric cancer remains a prevalent malignancy, with molecular drivers critical for its progression and therapeutic targeting. Recently, some malignancies have been linked to the lncRNA ZFAS1, but its mechanism of action and the reasons for its dysregulation in gastric cancer are unknown.
Materials and Methods: We evaluated ZFAS1 expression in gastric cancer cells (SGC7901) by RT-qPCR and compared it with GES-1 cells. The LnCAR database provided insight into ZFAS1 levels in STAD compared to normal tissue. The effect of ZFAS1 knockdown on cell invasion, migration and proliferation was evaluated using transwell invasion, wound healing assays, CCK8 and flow cytometry. In addition, ZFAS1 promoter regions were examined using the JASPAR database and subsequent ChIP assays to understand SP1 transcription factor binding.
Results: The effect of ZFAS1 on the AKT/mTOR pathway was clarified using Western blotting. SGC7901 cells were shown to have increased ZFAS1 expression, which was linked to a poor prognosis for gastric cancer. Knockdown of ZFAS1 in SGC7901 cells inhibited cell invasion, migration and proliferation and induced apoptosis. In addition, SP1 was found to upregulate ZFAS1 transcription by binding to its promoter region. ZFAS1 knockdown resulted in a significant reduction of key proteins (p-AKT, AKT, p-mTOR, and mTOR) of the AKT/mTOR pathway. When the AKT activator SC79 was introduced, the repressive effects of ZFAS1 knockdown on cell invasion, migration, proliferation, and AKT/mTOR signaling were partially reversed.
Conclusion: Our results highlight the pivotal role of ZFAS1 in gastric cancer cell malignancy, which is modulated by the AKT/mTOR pathway.
PP-01-235
Amino Acid Metabolism Related Genes Model For Prognosis And Immunity In Gastric Cancer
Huan Zhang1, 女士 Lu Qang2 and 先生 Hailong Cao1
1Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, tianjin, China;2Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To systematically investigate amino acid metabolic reprogramming in gastric cancer, construct prognostic models, and validate gene signatures for predictive value and clinical decision-making.
Materials and Methods: Downloaded data; constructed amino acid metabolism prognostic models. Analyzed model genes' prognosis and clinical correlations; identified TCGA-STAD DEGs. Functional Enrichment Analysis (GO), GSEA\GSVA enrichment analysis; PPI network and Constructed the interaction network of mRNA-miRNA, mRNA-TF and mRNA-RBP. Analyzed immune infiltration; built and correlated clinical prognostic models. Processed single cell data post-quality control; annotated cell types. Scored cells with AUCell; validated with RNA-RT-qPCR; conducted statistical analysis.
Results: Significant negative correlations were identified between immune cells (T cells CD4 memory activated) and the expression of 8 hub genes in the TCGA-STAD dataset. Relationships between the 8 AAM-related hub genes and immune cells were analyzed using the scRNA-seq dataset GSE184198 from GC patients. Elevated expressions of the 8 hub genes in gastric cancer cells were confirmed via PCR. These genes were utilized to construct a genetic predictive model for personalized outcome prediction in gastric cancer patients.
Conclusion: We developed an AAMRMGs prognostic model that assesses molecular signatures and the strength of infiltrative immune cells to classify AAM clusters. Risk profiles derived from hub genes were validated as independent prognostic indicators for gastric cancer, indicating a link between amino acid metabolism-related genes and patient outcomes. Cellular heterogeneity was characterized by associating hub genes with immune cells. These findings offer novel insights for survival prognostication and immunotherapy strategies in gastric cancer.
PP-01-236
Identification and Regulation of Exhausted T Cell Subpopulations in CRC Immune Microenvironment Using Single-Cell Genomics
博士 Luping Zhang and Hong Xu and Nan Zhang and Ying Li
Department of Gastroenterology, The First Hospital of Jilin University, Changchun, China, Changchun, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Colorectal cancer (CRC) is a major gastrointestinal malignancy in China. Over 30% of advanced CRC patients experience recurrence or metastasis post-surgery. Adjuvant immunotherapy offers hope but is hindered by T cell exhaustion. This study aims to identify and characterize exhausted T cell subpopulations in CRC using single-cell genomics and explore mechanisms to reverse T cell exhaustion and enhance immunotherapy efficacy.
Materials and Methods: Single-cell RNA sequencing (scRNA-seq) analyzed the immune microenvironment of CRC tissues from advanced-stage patients. Exhausted T cells (Tex cells) were identified by specific surface markers and gene expression profiles. Bioinformatics analyses elucidated regulatory pathways and transcription factors involved in T cell exhaustion. Validation used flow cytometry and functional assays to assess T cell activity and cytokine production.
Results: scRNA-seq identified Tex cell subpopulations with high expression of inhibitory receptors like PD-1, Tim-3, and Slamf6. Key transcription factors, including NFAT, Nur77, and BACH2, drove the differentiation of stem-like CD8+ Tex cell progenitors. These progenitors showed reduced antigen dependency and maintained stem-like properties through TCF-1, Eomes, and Bcl-6 expression, while antagonizing effector-related transcription factors like Blimp-1 and T-bet. Functional assays showed that targeting these pathways partially restored T cell activity and enhanced anti-tumor responses.
Conclusion: Our study provides insights into T cell exhaustion in CRC and highlights mechanisms to reverse exhaustion. These findings could inform optimized adjuvant immunotherapy strategies, offering new avenues for effective CRC treatment.
PP-01-237
Biofeedback Therapy is effective in all types of Pelvic Floor Dyssynergia: A retrospective cohort study
1Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, India;2Department of Physiology, All India Institute of Medical Sciences, New Delhi, India;3Department of Gastroenterology, All India Institute of Medical Sciences, Jodhpur, India;4Department of Gastroenterology, Indira Gandhi Medical College and Hospital, Shimla, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The data on effectiveness of biofeedback therapy (BFT) among different types of Pelvic floor dyssynergia (PFD) in Indian population is limited. This study aimed to evaluate the effect of BFT on clinical and manometric parameters in PFD.
Materials and methods: Consecutive adult patients with PFD who completed 6 sessions of anorectal manometry-based BFT at a tertiary care center in New Delhi, India were included in this retrospective cohort study. Demographic, clinical, and manometric parameters of the patients before and after BFT were evaluated. Primary outcome was the change in Cleveland clinic constipation score (CCCS), and secondary outcomes were changes in clinical symptoms and manometric parameters in different types of PFD, and baseline predictors of positive response to BFT.
Results: Out of 240 patients who underwent BFT, 63 patients completed 6 sessions and were included: 41 (65.1%) were males with median age of 39 years (IQR 27-55 years). Type 3 (26, 41.2%) and type 1 (22, 34.9%) were most common followed by type 4 (10, 15.9%) and type 2 (5, 7.9%) PFD. There was significant improvement in CCCS after BFT (pre-therapy 7(4-8) to post-therapy 4 (2-6), p value <0.001). Improvement of ≥25% on visual analog scale in overall symptoms was observed in 47 (74.6%) patients. There was significant improvement in individual clinical symptoms and anal relaxation%. There was no significant predictor and difference in outcomes among different types of PFD according to Rao or Rome IV classification.
Conclusion: Biofeedback therapy is effective in all types of PFD in Indian population.
PP-01-238
Supplementation of Lactobacillus helveticus NS8 alleviated an endogenous rat depression
1Akita University, Akita City, Japan;2MIRAI Foods Institute, Yokote-shi, Akita City, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Depressive disorder is a condition involving microbiota-gut-brain axis dysfunction. Increasing studies have attempted to improve depression through gut-microbiota regulation. However, most studies have focused on chronic stress or drug-induced exogenous depression, with only limited research on endogenous depression and gut-microbiota. This study aims to explore roles of gut-microbiota in endogenous depression, whether genetic background affects probiotic intervention, and how microbiota affects behavior.
Methods: Wistar-Kyoto rats were used as endogenous depression models, with Wistar rats as controls. The differences in behavior, gut-microbiota, nervous system, endocrine system, immune system, and gut-barrier between two types of rats were evaluated. The effect of supplementing with Lactobacillus helveticus NS8 was studied.
Results: Wistar-Kyoto rats exhibited abnormal gut-bacteria and mycobiota, characterized by higher bacterial α-diversity but lower fungal α-diversity, and dysfunction in the microbiota-gut-brain axis, as evidenced by a hypo-serotonergic system, hyper-noradrenergic system, defective hypothalamic-pituitary-adrenal axis, compromised gut-barrier integrity, heightened serum inflammation, and diminished gut immunity. The intervention with NS8 not only increased the fecal abundance of L. helveticus but also decreased depression-like behaviors, reduced levels of corticotropin-releasing hormone in hippocampus, increased levels of brain-derived neurotrophic factor, dopamine, and glucocorticoid receptor in the hippocampus, improved the gut-microbiota, serotonergic and noradrenergic systems.
Conclusion: The depressive phenotype of Wistar-Kyoto rats is not only attributed to their genetic context but also closely related to their gut microbiota. Like exogenous depression, abnormal gut microbiota and dysfunctional microbiota-gut-brain axis play important roles in endogenous depression. Supplementing probiotics of NS8 is probably a promising way to improve endogenous depression.
PP-01-239
Improvement of Cognitive Function Following Fecal Microbiota Transplantation
Jung Hwan Lee, Jongbeom Shin, Boram Cha, Ji-Taek Hong and Kye Sook Kwon
Inha University Hospital, Incheon, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Fecal microbiota transplantation (FMT) has emerged as a promising therapeutic intervention for various gastrointestinal and metabolic disorders. Recent studies suggest potential benefits of FMT on cognitive function, possibly through the gut-brain axis. This study aims to evaluate the impact of FMT on cognitive function in patients with cognitive impairment, using the Mini-Mental State Examination (MMSE).
Materials and Methods: This prospective study followed 7 patients with cognitive impairment, excluding severe cases (MMSE score = 0, 6 patients). Participants underwent FMT via colonoscopy and were assessed at baseline and three months post-transplantation. Cognitive function was evaluated using the MMSE and secondary assessments including the Montreal Cognitive Assessment (MoCA).
Results: Post-FMT, median MMSE scores changed, with the median score increasing from 12 [3-18, interquartile range] at baseline to 11 [9-16] after one week. Excluding 4 patients with no follow-up at one month, median MMSE scores of 3 patients increased from 1 [1-12] to 10 [9-18]. The MMSE score of 1 patient who followed up at 3 months increased from 23 to 28. Improvements were also observed in the median MoCA score from 0 [0-6] to 10 [9-18] after 1 month. In the microbiome study, Bifidobacterium, Ruminococcus gnavus, Collinsella, and Coprococcus genus increased after FMT.
Conclusion: FMT appears to improve cognitive function in patients with cognitive impairment, as evidenced by increases in MMSE and MoCA scores along with changes in gut microbiota. These findings suggest that modulation of the gut microbiota may have therapeutic potential in managing cognitive decline.
PP-01-240
Frequency of Overlap of Functional Dyspepsia and Functional Bowel Disorders in the Clinical Setting
Shahana Parvin, Karzon Dey Sarker, Rashedul Hasan, Golam Kibria and M Masudur Rahman
Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Overlap of disorders of gut-brain interaction (DGBI) has a negative impact on treatment outcome due to ineffective treatment approach. Overlap of these conditions is common in population-based studies, but clinical data are lacking. The aims of this study were to determine the frequency of overlap between functional dyspepsia (FD) and functional bowel disorders (FBD).
Materials and Methods: Consecutive DGBI patients based on the ROME lV diagnostic criteria attending at the outdoor patient department in Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh were interviewed using validated Bengali ROME lV questionnaire and categorized based upon the symptom pattern as having FD, FBD or FD/FBD overlap.
Results: Of 2,227 patients, 595 (26.72%) had DGBI [(mean age 34.81 ± 1.11 years; male 399 (67.1%)], functional esophageal, gastroduodenal, bowel disorders and anorectal disorders were present in 154 (25.9%), 359 (60.3%), 566 (95.1%) and 17 (2.9%), respectively. Most common DGBI were functional dyspepsia (FD) 267 (44.9%) followed by irritable bowel syndrome (IBS) 174 (29.2%), functional constipation (FC) 151 (25.4%) and functional diarrhea (FDr) 131 (22.0%). Overlap between FD and FBD were present in 197(33.1%). Among them, FD-IBS, FD-FC and FD-FDr overlap were present in 87(14.6%); 67(11.3%) and 54(9.1%) patients respectively.
Conclusion: Roughly a third of patients fulfils the Rome IV criteria of DGBI. Around half of them have FD and one third of DGBI patients have FD-FBD overlap. Therefore, symptom misinterpretation may lead to treatment dissatisfaction. FD-FBD overlapping patients require multiple targeted combination treatment rather than specifically targeted treatment.
PP-01-241
Prophylactic treatment of cycling vomiting syndrome in children with whole food plant-based diet and probiotics
Priyanka Udawat1 and HARSHKUMAR Patel2
1Sir H N Reliance Foundation Hospital, Mumbai, India;2Consultant, Pediatric Neurologist, Ahmedabad, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To describe the utility of the combination of Whole Food Plant Based (WFPD) and probiotics (bifidobacterium lactis 10 billion CFU and Lactobacterium plantis 1.5 billion CFU) in the prophylactic treatment of cycling vomiting syndrome. (CVS)
Methods: During the study period of June 2022 to march 2024; 33 children with CVS (diagnosed based on north American society paediatric gastroenterology, hepatology and nutrition criteria) studied. Acute episodes in children with CVS were managed as per standard protocols and for chronic prophylaxis they were given combination of WFPD and probiotics for 3 months duration with followup visits at 1,2,4 and 6 months of starting the treatment.
Results: Out of 33 children 19 (57%) were boys and 14 were girls (43%) with their mean age 4.7 + 3.4 years. 18 of them (group A) were given combination of WFPD and probiotics along with standard prophylactic treatment (eg amitryptilin or topiramate) while 15(Group B) were kept only on combination of WFPD and probiotics. Both cohorts showed comparable reduction in frequency of acute episodes over six months after starting the treatment. Mean average of total episodes 1.3 + 0.4 and 1.2 + 0.2 in group A and group B respectively. (p value>0.05). Quality of life reported by parents comparable in both groups additionally in group B parents more relaxed due to they didn’t have to worry for drug related side effects.
Conclusion: Combination of WFPD and probiotics demonstrated comparable efficacy and better tolerability compared to standard treatment as prophylactic treatment in children with CVS.
PP-01-242
Modulation gut brain axis with whole plant-based diet in children with autism spectrum disorder
Priyanka Udawat1 and Harshkumar Patel2
1Sir H N Reliance Foundation Hospital, Mumbai, India;2Consultant, Ahmedabad, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To demonstrate the efficacy and acceptability of whole food plant based diet (WFPD) as a dietary intervention in children with autism spectrum disorder aged 2-15 years.
Material/Methods: Children were diagnosed autism spectrum disorder on the basis of DSM 5 and severity score was calculated with CARS 2 (Childhood autism rating scale). During study period of June 2023-November 2023 total 118 children were studied. Minimum 6 moths of follow up with monthly visits were obtained with CARS assessment done at 2 and 6 months visits
Results: During study period total 158 children were enrolled but finally 118 children completed the required minimum six months of follow-up. Out of 118; 66 (56%) were boys and 52 (44%) were girls with mean age of 4.5 + 3.7 years. WFPB is made up of fruits, vegetables, whole grains legumes, nuts and seeds, and excludes animal foods. WFPB was advised and counselled to parents as per their children’s need by the team of Paediatric gastroenterologist, Lifestyle Medicine Physician & dieticians. Follow-up visits, assessment monitored by paediatric neurologist & therapists. All 118 children followed up for minimum 6 months with WFPB diet along with standard therapy plan. The improvement in CARS scores from baseline to 9.5 + 3.2 and 15.2 + 2.9 at 2 and 6 months.
Conclusion: WFPD is simple and effective dietary intervention without the risk of nutritional compromise for the children with ASD aged 2-15 years. It shows significant improvement after implementation with increasing benefits over the use of longer duration.
PP-01-243
Dynamic changes in the gut microbiota after bismuth quadruple and high-dose dual therapy for H.pylorieradication
Jing Chen, Yan Zhang, Hanchen Min, Junli Zhi, Shuyun Ma, Hongxia Dong, Jingshuang Yan, Xiaoyan Chi, Xiaomei Zhang and Yun-Sheng Yang
The First Medical Center Of The General Hospital Of The Pla, Beijing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate the impact of high-dose dual therapy(HDDT )on the gut microbiota and compare it with that of bismuth quadruple therapy (BQT).
Materials and Methods: We enrolled outpatients (18–70 years) diagnosed with Helicobacter pylori infection by either histology or a positive 13C-urea breath test (13C-UBT) and randomly assigned to either the BQT or HDDT group. Subjects consented to provide fecal samples which were collected at baseline, Week 2, and Week 14. Amplification of the V1 and V9 regions of the 16S rRNA was conducted followed by high-throughput sequencing.
Results: Ultimately, 78 patients (41 patients in the HDDT group and 37 in the BQT group) were enrolled in this study. Eradication therapy significantly altered the diversity of the gut microbiota. However, the alpha diversity rebounded only in the HDDT group at 12 weeks post-eradication. Immediately following eradication, the predominance of Proteobacteria, replacing commensal Firmicutes and Bacteroidetes, did not recover after 12 weeks. Species-level analysis showed that the relative abundances of Klebsiella pneumoniae and Escherichia fergusonii significantly increased in both groups at Week 2. Enterococcus faecium and Enterococcus faecalis significantly increased in the BQT group, with no significant difference observed in the HDDT group. After 12 weeks of treatment, the relative abundance of more species in the HDDT group returned to baseline levels.
Conclusion: Eradication of H. pylori can lead to an imbalance in gut microbiota. Compared to BQT, the HDDT is a regimen with milder impact on gut microbiota.
PP-01-244
Diagnostic accuracy of pylori duotect (for flid and caga) in patients with upper gastrointestinal symptoms
Viet Hang Dao and Phuc Binh Nguyen and Thi Thao Dang and Thi Huyen Trang Nguyen and Thu Thuong Nguyen and Thi Thu Uyen Vu and Van Long Dao
Hanoi Medical University, Hanoi, Viet Nam;Institute of Gastroenterology and Hepatology, Hanoi, Viet Nam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate diagnostic accuracy of H.pylori detection using blood test with FliD and CagA antibodies (Pylori Duotect) in Vietnamese patients with upper GI symptoms.
Methods: A cross-sectional study was conducted at the Institute of Gastroenterology and Hepatology in Hanoi, Vietnam, focusing on adults from 18 years old with upper GI symptoms without history of treated peptic ulcers or H.pylori eradication. Participants were indicated with upper GI endoscopy and three methods to diagnose H.pylori infection: RUT, UBT, and Pylori Duotect. Patients were considered H.pylori positive if both RUT and UBT were positive. The parameters for evaluating the diagnostic accuracy of Pylori Duotect include sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV).
Results: From December 1, 2023, to June 20, 2024, 272 patients were recruited with the mean age of 46.7 years old, 58.5% were female. The most common symptom was epigastric pain (47.4%). There were 92.7% of patients with gastritis, 12.1% with gastric ulcers, 8.1% with duodenal ulcers. 49.3% of patients were positive with Pylori Duotect test (FliD: 19.4% and CagA: 43.0%). Based on RUT and UBT results, 42.3% of patients were diagnosed with H.pylori infection. Pylori DuoTect’s sensitivity, specificity, PPV, and NPV were 80.0%, 64.7%, 50.7%, and 87.7%, respectively.
Conclusion: The Pylori DuoTect test had low sensitivity and specificity in diagnosis H.pylori infection in Vietnamese patients with upper gastrointestinal symptoms.
PP-01-245
Evaluation of anxiety about gastric cancer by cancer worry scale
Akita University Graduate School of Medicine, Akita, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To elucidate the factors that influence fear and anxiety about Gastric cancer based on Helicobacter pylori infection status is the object.
Materials and Methods: This study was conducted as a secondary evaluation of a previous study (Fukuda S, et al. Den Open. 2024;4:e329.). The survey was distributed questionnaires to participants who underwent health checkups at three facilities in Akita Prefecture between May 2021 and March 2022. The questionnaire items were Cancer Worry Scale(CWS), SF-8, and FSSG. CWS was used to assess anxiety about gastric cancer.HP infection status was ascertained from medical records.
Results: There were 303 subjects in the study, with a median CWS of 14 (IQR:12, 17). The median CWS were 14(12, 16) in HP negative , 14(10.8, 16)in HP positive, and 15(12, 17) in HP eradicated. No statistically significant differences were found. In addition, the HP-eradicated group continued to have high anxiety levels after the eradication of HP. The logistic regression analysis results show that positive FSSGs and subnormal MCS are associated with high CWS scores.
Conclusion: Regardless of HP infection status, many participants expressed high anxiety about GC. Anxiety levels remained particularly high in the HP-eradicated group. These results indicate problems with current age criteria-based GC screening, and appropriate patient education is needed when risk-based screening is introduced.
PP-01-246
Fourteen-day amoxicillin- or tetracycline-containing bismuth quadruple therapy for clarithromycin resistant Helicobacter pylori infection
Samryong Jee and Cheol Woong Choi and Myeongseok Koh and Sung Eun Kim and Bong Eun Lee and Jin Lee and Ra Ri Cha
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Due to the increase in clarithromycin resistance, the eradication rate of Helicobacter pylori is decreasing. Through this study, we compared the success rate of eradication with PAM (PPIs, amoxicillin and metronidazole) regimen and PBMT (PPIs, bismuth, metronidazole and tetracycline) regimen by adding bismuth to PAM regimen.
Materials and Methods: This prospective multicenter study compared the eradication rates among B-PAM (bismuth 300mg four times, rabeprazole 20mg, amoxicillin 1g, and clarithromycin 500mg twice daily), PAM, and PBMT (rabeprazole 20mg twice, metronidazole 500mg three times and tetracycline 500mg with bismuth 300mg four times daily) regimens for 14 days. From December 2022 to February 2024, a total of 198 patients were enrolled at seven medical institutions in Busan, Ulsan, and Gyeongnam regions of South Korea. To diagnose the presence of clarithromycin resistance, we conducted DPO-PCR method.
Result: A total 170 patients were included in the PP analysis. When comparing the eradication rates among the groups, the B-PAM group demonstrated a similar rate of 96.5% compared to the PBMT group (94.6%, p=0.633), while statistically significant differences were observed when compared to the PAM group (75.4%, p=0.001). The B-PAM regimen did not show significant differences in terms of side effects compared to the PAM and PBMT regimens. Additionally, symptoms of nausea and vomiting were less frequent in the B-PAM group compared to the PBMT group (p = 0.007).
Conclusion: B-PAM regimen could be recommended as an initial treatment for Helicobacter pylori infections resistant to clarithromycin.
PP-01-247
Efficacy of P-cab based bismuth-containing triple therapy as the first-line eradication regimen for H.pylori infection
Dong-kyu Lee
Daejeon Eulji Medical Center, Eulji University, Daejeon, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The eradication rates of clarithromycin-containing triple regimens for Helicobacter pylori have declined. A potassium-competitive acid blocker (P-CAB) based therapy with added bismuth is expected to be effective due to its strong acid inhibition. This study compares the efficacy of a 14-day P-CAB-based bismuth-containing triple regimen (PC-BAC) with a 14-day PPI-based bismuth-containing triple regimen (P-BAC) in patients with high clarithromycin resistance.
Methods: We retrospectively reviewed data of patients who received first-line treatment for H. pylori with either PC-BAC (50 mg tegoprazan + 300 mg bismuth subcitrate potassium + 1000 mg amoxicillin + 500 mg clarithromycin twice daily for 2 weeks) or P-BAC (40 mg pantoprazole + 300 mg bismuth subcitrate potassium + 1000 mg amoxicillin + 500 mg clarithromycin twice daily for 2 weeks). H. pylori eradication rates and adverse events were compared between the two groups.
Results: A total of 143 patients were enrolled, including 60 patients in the PC-BAC group and 83 patients in the P-BAC group. The eradication rates were 84.7% in the PC-BAC group and 66.7% in the P-BAC group. The eradication rate was significantly higher in the PC-BAC group than in the P-BAC group (p=0.03). There was no statistical difference in the frequencies of adverse events between PC-BAC and P-BAC (p=0.38).
PP-01-248
Individual vs. Family-Based Screening for Helicobacter pylori Infection: A Population-Based Study
1National Taiwan University Hospital, Taipei, Taiwan;2Health Promotion Administration, Ministry of Health and Welfare, Taipei, Taiwan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Screening and treating Helicobacter pylori is an effective strategy to enhance stomach health. The family index-case method, which involves screening family members of those who test positive, may improve screening effectiveness. This study tested whether this approach also boosts screening quality indicators in a high-risk gastric cancer population.
Materials and Methods: From September 24, 2018, to December 31, 2023, 19,151 participants from 55 indigenous Taiwanese communities underwent H. pylori testing using 13C-urea breath tests in an organized screening program. Among them, 6,825 were enrolled using the family index-case method and 12,326 through the usual method. We compared the positivity rates, refer-to-treatment rates, compliance-to-treatment rates (≥80% medication use), eradication rates, and reinfection rates between the two methods using generalized estimated equation models. Results were expressed as adjusted odds ratios (aORs) or rate ratios (RRs) with 95% confidence intervals (CIs).
Results: The family index-case method reached 1,721 family members (143%) from 1,199 positive index cases, increasing the H. pylori detection rates (72.9% vs. 41.1%, aOR: 1.50, 95% CI: 1.43-1.56). The refer-to-treatment rates (77.3% vs. 75.1%, aOR: 1.03, 95% CI: 0.98-1.09), compliance-to-treatment rates (82.9% vs. 82.5%, aOR: 1.03, 95% CI: 0.90-1.17), and the eradication rates (78.4% vs. 81.0%, aOR: 0.98, 95% CI: 0.95-1.00) were similar between the two methods. The reinfection rate was lower in the family index-case method (0.51% vs. 1.30% per person-year, RR: 0.39, 95% CI: 0.08-1.82) than the usual screening method.
Conclusion: The family index-case method increases the H. pylori detection rates and may reduce the reinfection rates.
PP-01-249
Efficacy of metronidazole-containing rescue regimens after standard triple therapy for Helicobacter pylori: A meta-analysis
1Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;2Department of Gastroenterology and Hepatology, International University of Health and Welfare, Narita Hospital, Narita, Japan;3Center for Diagnostic and Therapeutic Endoscopy, Keio University School of Medicine, Tokyo, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Although standard triple therapy is still used as the first-line eradication treatment worldwide, it is unclear whether metronidazole should be included empirically in the second-line eradication treatment. The aim of this study is to compare the efficacy of metronidazole-containing regimens and metronidazole-free regimens after the failure of first-line eradication with standard triple therapy.
Materials and Methods: PubMed, the Cochrane library, and the Igaku-Chuo-Zasshi database were searched in order to identify RCTs eligible for inclusion in the systematic review and the meta-analysis. The odds ratios (ORs) with 95% confidence intervals (CIs) were calculated.
Results: Thirteen eligible RCTs were included. All 13 trials included a total of 2039 patients assigned to metronidazole-containing (975 patients) or metronidazole-free (1064 patients) regimens. Metronidazole-containing regimens had significantly lower eradication failure rates compared to regimens without metronidazole (OR 0.55; 95%CI 0.39–0.78). Subgroup analysis based on regional risk of metronidazole resistance demonstrated that the metronidazole-containing regimens had lower eradication failure rates not only in low-risk regions but also in high-risk regions (OR 0.29; 95%CI 0.11–0.74 and OR 0.66; 95%CI 0.49–0.91).
Conclusion: After the failure of standard triple therapy, secondary eradication treatment regimens containing metronidazole demonstrated a higher eradication rate compared to those without metronidazole.
PP-01-250
Effect of macrolide exposure on tailored Helicobacter pylori eradication therapy and antibiotic resistance profiles
Jin Hee Noh1, Kee Don Choi2, Hee Kyong Na2, Ji Yong Ahn2, Jeong Hoon Lee2, Kee Wook Jung2, Do Hoon Kim2, Ho June Song2, Gin Hyug Lee2 and Hwoon-Yong Jung2
1Hallym University Sacred Heart Hospital, Anyang, South Korea;2Asan Medical Center, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Determining antibiotic use history accurately is challenging due to its reliance on patient recall. By determining macrolide exposure using the Korean Drug Utilization Review (DUR) system, we analyzed the impact of previous macrolide use on antibiotic resistance profiles and compared the eradication rate between tailored therapy based on macrolide exposure history and empirical therapy.
Materials and Methods: Patients with confirmed Helicobacter pylori (H. pylori) infection who agreed to access prescription information using the Health Insurance Review and Assessment Service DUR system were enrolled between 2021 and 2023. Patients received tailored therapy, which was clarithromycin (CLR)-based triple therapy in cases without macrolide exposure and bismuth quadruple (BQ) therapy in cases with macrolide exposure. The empirical therapy group was prospectively recruited at the same time to compare the eradication rate.
Results: A total of 418 patients (tailored therapy group, n=57; empirical therapy group, n=361) were analyzed. Among the tailored therapy group, 24.6% took macrolide antibiotics for the past 5 years. CLR resistance rates were higher in patients with previous macrolide use than in those without (66.7% vs. 7.5%, p<0.001). The tailored therapy group showed a higher eradication rate than the empirical therapy group for intention-to-treat (ITT), modified intention-to-treat (MITT), and per-protocol (PP) analyses (ITT, 86.0% vs. 75.6%; MITT, 94.2% vs. 80.3%; PP, 94.2% vs. 85.1%).
Conclusion: Previous macrolide exposure identified using the DUR system was associated with a higher rate of CLR resistance. Tailored therapy based on macrolide exposure history led to higher eradication rates compared to empirical therapy.
PP-01-251
The effectiveness of Vonoprazan-based regimens for Helicobacter pylori eradication in China: a network meta-analysis
Cangui Zhang1, Bingyun Lu2, Li Xie3, Xi Ran3 and Ye Chen2
1Nanfang Hospital, Southern Medical University, Guangzhou, China;2Shenzhen Hospita of Southern Medica University, Shenzhen, China;3Takeda Pharmaceutical Company Ltd, 200126, Shanghai, China
Objectives: This network meta-analysis (NMA) aims to evaluate the effectiveness of Vonoprazan (VPZ)-based regimens and their performance against proton pump inhibitor (PPI)-based treatments for Helicobacter pylori eradication, with a focus on results from the subgroup of Chinese patients.
Materials and Methods: We included randomised controlled trials (RCT) published in PubMed, EMBASE, the Cochrane Library, web of science, CNKI, Wanfang, VIP and CBM from inception to 9th January, 2024, investigating VPZ- and PPI- based dual, triple and quadruple treatments in adult patients. Each RCT comprised at least one arm receiving VPZ-based regimens. The eradication rate of VPZ and PPI based regimens was analysed using proportional meta-analysis. NMA employing a Bayesian hierarchical random-effects model was performed. Subgroup analysis was conducted in Chinese patients using the same methods as the main analysis.
Results: Among the 45 included studies in this review, 19 studies with 2508 patients provided information for Chinese subgroup (Figure 1). The eradication rate was 90.85% for VPZ-quadruple, 87.81% for VPZ-triple, 86.36% for VPZ-dual, 82.04% for PPI-quadruple, 78.72% for PPI-triple, and 84.38% for PPI-dual in China. In terms of surfaces under cumulative ranking curve (SUCRA), VPZ-quadruple demonstrated the potential to be the most optimal treatment (SUCRA=83.26%), followed by VPZ-triple (SUCRA=79.17%) and VPZ-dual (SUCRA=68.35%). For Chinese patients, VPZ-quadruple, triple and dual regimens showed significantly higher eradication rates than PPI-triple regimens. VPZ-quadruple and dual regimens was superior to PPI-quadruple (Table 1).
Conclusion: In China, VPZ-based regimens demonstrated benefits on eradication compared to PPI quadruple and PPI-triple regimens, with VPZ-quadruple being the most effective.
PP-01-252
Effect of H. pylori eradication therapy on metabolic parameters in non-alcoholic fatty liver disease patients
Frinz Moey Caunes Rubio1, Cyra Kaye Cuecaco Mina1, Kevin Rigor Sese2, Jasper Ian Amparo Alindayu3, Kevin Lloyd Bedico Aboy3 and Fred Lawrence Delantar Samante3
1St. Luke's Medical Center - Quezon City, Quezon City, Philippines;2St. Luke's Medical Center - Global City, Taguig City, Philippines;3University of the Philippines - College of Medicine, Manila City, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Recent meta-analyses confirm that Helicobacter pylori infection increases the risk of non-alcoholic fatty liver disease due to its association with insulin resistance. However, no meta-analysis has evaluated the effect of H. pylori eradication therapy on metabolic indices in non-alcoholic fatty liver disease patients. This study conducted a systematic review and meta-analysis of randomized controlled trials assessing the impact of H. pylori eradication therapy on patients with H. pylori infection and non-alcoholic fatty liver disease.
Materials and Methods: A comprehensive search of randomized controlled trials in PubMed, Cochrane, and Google Scholar was conducted on H. pylori eradication therapy for patients with H. pylori infection and non-alcoholic fatty liver disease. Outcomes measured included triglyceride, fasting blood glucose, body mass index, total cholesterol, low-density lipoprotein, high-density lipoprotein, and homeostatic model assessment for insulin resistance. Analyses were performed with Review Manager version 5.4.
Results: Three studies with 329 subjects were included in the meta-analysis. H. pylori eradication therapy plus standard-of-care reduced triglyceride levels by 20.97 mg/dL (95% CI: 0.01 to 41.94) compared to standard-of-care alone. Due to substantial heterogeneity (I²=69%), a random effects model was used. Other outcomes showed no significant differences between treatment and control groups.
Conclusion: This meta-analysis indicates that adding H. pylori eradication therapy to standard-of-care significantly reduces triglyceride levels compared to standard-of-care alone. However, heterogeneity suggests effect variability across studies. The findings underscore the need for further clinical trials to evaluate the effectiveness of H. pylori eradication therapy on the metabolic parameters of patients with non-alcoholic fatty liver disease.
PP-01-253
Correlation Of Gastric Cancer And Helicobacter-Pylori-Positive Patients Admitted At Amai Pakpak Medical Center From 2016-2022:Retrospective-Study
Sittie Johaynee Sampaco and Abdel Hussein Lucman
Amai Pakpak Medical Center, Marawi City, Philippines
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Gastric cancer (GC) is a major health concern worldwide and is responsible for numerous cancer-related deaths. Despite declining rates of GC in western regions in the reports of Arnold et al. In Lanao del sur, there is still no reported statistical data with regards to the association of GC and Helicobacter pylori (H. pylori) infection. Diagnostic challenges and limited access to tests exacerbate underdiagnosis. The study aimed to identify the prevalence of GC in patients admitted at APMC diagnosed with H. pylori infection and to correlate between GC in H. pylori-positive patients.
This retrospective study explored the relationship between gastric cancer and H. pylori-positive patients at APMC (2016-2022). This study reviewed the patients' medical records to unveil demographic profiles, assess correlations between gastric cancer and its associated risk factors, including H. pylori-positivity.
The results showed that there is an increasing prevalence of gastric cancer among older individuals with H. pylori infection with male predominance of about 70.4% (18 out of 27). The association between comorbidities such as hypertension, diabetes mellitus, liver cirrhosis, etc. and gastric cancer in H. pylori-positive individuals lacks substantial evidence. However, overall, there is a significant association between gastric cancer and H. pylori positivity (p-value of 0.000).
In conclusion, this study showed that H. pylori infection is an independent risk factor for gastric cancer. Furthermore, the prevalence rate of gastric cancer among H. Pylori positive patients is 8.11% emphasizing the need for comprehensive care strategies and further studies to refine prevention approaches and elucidate demographic associations.
PP-01-254
Ulcer outside and inside - Effective strategies of managing Crohn’s disease associated Pyoderma Gangrenosum
Lok Ka Lam
Queen Mary Hospital, Hong Kong
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Pyoderma gangrenosum (PG) is an uncommon inflammatory ulcerative skin lesion that is frequently associated with inflammatory bowel disease (IBD). We aim to discuss the stepwise approach on the management of PG related to Crohn’s disease (CD).
Case Description: We report a classical case of CD-associated PG in a 75-year-old woman with persistent leg ulcer that was refractory to conventional steroids, cyclosporin A, azathioprine and infliximab. She was then administered adalimumab and her ulcer healed after four months of treatment. The clinical presentation, investigation modality, and choice of treatment throughout her one-year disease course is highlighted in Figure1.
Discussion: Our case has demonstrated a good response to adalimumab in which objective clinical improvement could be seen at two months and complete healing at four months. Adalimumab has few advantages. First, adalimumab is administered subcutaneously and could be taken at home. Second, there were less allergic reactions as adalimumab is a fully human anti-TNF-α antibody.
We have proposed a management algorithm for CD-associated PG (Figure2). In mild disease, therapy consists of local wound care and topicals. For more severe disease, systemic agents are necessary. These include initial induction with prednisolone, together with steroid-sparing agents such as azathioprine. The second line treatment will be infliximab. If refractory, adalimumab can be considered. Whether newer biologics, such as ustekinumab or small molecules, are effective will need further data to support. Before choosing a therapy, the following should be taken into consideration: medical history, presence of concomitant systemic disease, severity of ulcers, and disease course.
PP-01-255
Correlation between SCFA, Fecal Calprotectin, Systemic Inflammation and CD4 Level in HIV Patients on ARV
Randy Adiwinata1,2, Agung Nugroho3, PN Harijanto3, Bradley Jimmy Waleleng4, Fandy Gosal4, Luciana Rotty4, Jeanne Winarta4, Andrew Waleleng4, Marcellus Simadibrata5 and Erni Juwita Nelwan6
1Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;2Gastrointestinal Cancer Center, MRCCC Siloam Hospital Semanggi, Jakarta, Indonesia;3Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;4Division of Gastroenterology and Hepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. dr. R. D. Kandou Hospital, Manado, Indonesia;5Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia;6Division of Tropical and Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To find the correlation between gut short chain fatty acid (SCFA), fecal calprotectin (FC), high-sensitivity C-reactive Protein (hsCRP), and CD4+ cell count in HIV patients on antiretroviral (ARV) and undetectable viral load.
Materials and Methods: This observational analytical-study was conducted at Prof. Dr. R. D. Kandou Hospital. We included HIV patients receiving first-line ARVs (tenofovir-lamivudine-efavirenz) regularly for at least one year and had undetectable viral load in past 6 months. Patients were evaluated for SCFA, fecal calprotectin level, hsCRP, and CD4+. Spearman correlation test was used to assess the correlation between variables.
Results: Twenty-five patients were included, 65% were male, with median age 33 (IQR 27.5-40.75) years, median ARV consumption duration 14 (12.75-18) months, median SCFA 13 (IQR 9.25-15) μmol/g, FC 16.45 (IQR 9.33-24.43) ug/g, hsCRP 1.6 (IQR 0.93-2.65) mg/L, and CD4+ 603.5 (IQR 443-792.75) cells/uL. Correlation test showed significant negative correlation between FC levels and CD4+ (r=-0.553;p=0.011), significant positive correlation between FC levels and hsCRP (r=0.448;p=0.047), and significant negative correlation between hsCRP levels and CD4+ (r=-0.540;p=0.014). No significant correlation between SCFA with other variables.
Conclusion: Decreased gut inflammation may lead to lower persistent systemic inflammation and higher CD4+ level in HIV patients on ARV, while gut inflammation may not be mediated by SCFA level.
PP-01-256
Extremely high prevalence of multidrug-resistant organisms in gut flora of hospitalised patients with gastro-intestinal illness
1Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, India;2Department of Laboratory Medicine, Trauma Centre, AIIMS, New Delhi, India;3Department of Microbiology, All India Institute Of Medical Sciences (AIIMS), New Delhi, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To find out prevalence of multidrug-resistant organism(MDRO) colonisation in hospitalised patients, and its concordance with MDR infections and mortality
Methods: We conducted a cross-sectional study at our tertiary-care centre in India. Rectal swab for bacterial culture was obtained from all consecutive patients admitted in our Gastroenterology ward and ICU over 2-month period (October-November 2022). Isolates were assessed for antibiotic sensitivity using VITEK 2 compact(BioMérieux Inc.). Organism was labelled “multi-drug resistant” based on non-susceptibility to any agent in at least 3 antimicrobial classes. Polymerase chain reaction (PCR) for antimicrobial resistance(AMR) genes was done using PCR system(Bio-RadTM).
Results: One hundred-seven patients with chronic liver disease and its complications (41.1%), acute pancreatitis(27.1%), inflammatory bowel disease(7.5%), acute cholangitis(6.5%) and other gastrointestinal disorders(17.8%) were included. Mean hospitalisation duration was 14+13 days, with 59.2%, 52.5% and 22.5% requiring central venous catheterization, Foley catheterization and mechanical ventilation, respectively. 106(99.1%) samples were positive for MDRO, predominantly E.coli(75.7%), Klebsiella pneumoniae(16.8%) and Enterococcus fecium(9.3%). NDM(New-Delhi-metallo-β-lactamase, carbapenemase)(64.5%) was most prevalent AMR gene, followed by TEM(extended-spectrum-beta-lactamase,ESBL)(58.9%) and OXA(carbapenemase)(55.1%). 15% of other clinical samples were positive for MDRO, most common pathogen being K.pneumoniae(50%). Concordance for MDRO was highest in acute pancreatitis (16.7% for E.coli and 33.3% for K.pneumoniae). Isolated gut MDR colonization was associated with 22%(n=20) mortality, while patients with MDRO in both stool and another clinical sample had 50%(n=8) mortality.
Conclusions: We report extremely high prevalence of gut colonisation with MDR organisms in hospitalised patients with GI illness. Urgent policy measures are needed to address the imminent threat of MDR infections.
PP-01-257
Kale improves chronic constipation through changes in the gut microbiome
Hirotada Akiho1, Mitsuru Esaki2 and Eikichi Ihara3
1Kitakyushu Municipal Medical Center, Kitakyushu, Japan;2Mayo Clinic Arizona, Phoenix, USA;3Kyushu University Hospital, Fukuoka, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Kale, known for its high fiber and isothiocyanates content, is anticipated to enhance bowel movement (BM) both directly and by improving the intestinal environment. We assessed the effects of Kale consumption on BM and gut microbiome in patients with mild Chronic Constipation (CC).
Materials and Methods: This was a prospective single-arm exploratory study. Fifteen patients with mild CC aged 50-90 were enrolled in this study. Each patient consumed a beverage containing 30 g of Kale twice a day for up to five weeks. Stool and colonic mucosa were collected at baseline and post-intervention (4 weeks after the start of Kale intervention) for 16S rRNA amplicon sequencing and metabolomic analysis. Patients recorded their daily BM. The study outcomes included the changes in BM frequency, gut microbiome and metabolome between the baseline and post-intervention.
Results: Median age of patients was 69 years. The overall Kale consumption rate was 94%. Significant improvement was observed in days with BM (+0.5/w, p<0.05), frequency of Spontaneous BM (+1.0/w, p<0.05) and Bristol Stool Scale (+1.0/w, p<0.001). Pairwise PERMANOVA test confirmed that stool microbiota was significantly different between patients with CC and healthy volunteers (p<0.05). Firmicutes to Bacteroidota ratio and Campylobacteria decreased in the mucosal microbiota (p<0.05). The concentration of fecal acetic acid significantly increased at post-intervention, and this was positively associated with the number of days with BM (P=0.063).
Conclusion: Kale induced improvement of BM in patients with mild CC, which may result from the changing gut microbiome and increasing acetic acid.
PP-01-258
Risk of LGI Bleeding in NSAID and Proton Pump Inhibitor Users Compared with NSAID-only Users
Jae Myung Cha and Moonhyung Lee
Kyung Hee University Hospital At Gangdong, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Recent studies have indicated an increased risk of lower gastrointestinal bleeding with the combined use of non-steroidal anti-inflammatory drugs (NSAIDs) and proton pump inhibitors (PPIs). We analyzed the comparative the risk of lower gastrointestinal bleeding between NSAID + PPI users and NSAID-only users.
Materials and Methods: This retrospective, observational study analyzed data from five hospitals using a common data model to investigate the risk of lower gastrointestinal bleeding between NSAID + PPI users (target cohort) and NSAID-only users (comparative cohort). Cox proportional hazard models and Kaplan–Meier estimations were employed after extensive propensity score matching.
Results: Among 24,530 and 57,264 individuals in the target and comparative cohorts, respectively, 8,728 propensity score-matched pairs were analyzed. The risk of lower gastrointestinal bleeding was significantly higher in the NSAID + PPI users than in the NSAID-only users (hazard ratio = 2.843, 95% confidence interval = 1.998–4.044; p < 0.001). Similar findings were also noted in elderly patients > 65 years (hazard ratio = 2.737), males (hazard ratio = 2.963), and females (hazard ratio = 3.221). However, the risk of lower gastrointestinal bleeding was comparable between NSAID + mucoprotective agent users and the NSAID-only users (hazard ratio = 2.057, 95% confidence interval = 0.714–5.924; p = 0.172).
Conclusion: The risk of lower gastrointestinal bleeding was higher in the NSAID + PPI users than the NSAID-only users. However, the risk of lower gastrointestinal bleeding was comparable between NSAID + mucoprotective agent users and the NSAID-only users.
PP-01-259
Bacterial D-ribose inhibits gastrointestinal motility through PAR2-p38 pathway in functional constipation
Nian Chen and Liping Guo and Bangmao Wang and Lu Zhou
Tianjin Medical University General Hospital, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The role of gut microbial metabolites has received increasing attention in gastrointestinal diseases. However, little is known about gut microbial metabolites in function constipation (FC). This study was designed to investigate the potential effects of bacterial metabolites on FC.
Materials and Methods: To identify differential metabolites between FC patients and controls, serum and stool samples were subjected to untargeted metabolomics. To confirm the candidate metabolites affecting the phenotypes of FC, these differential metabolites were perfused in intestinal segments. Further, the anti-diarrhea effects of metabolites were verified in senna leaf-induced diarrhea model.
Results: We identified 74 metabolites which were significantly up-regulated in serum or feces samples between FC patients and controls. Among these metabolites, D-ribose was detected in both serum and feces and displayed an apparent inhibition of the amplitude and frequency of spontaneous contraction in vitro perfusion of isolated intestinal segments of both humans and mice. In addition, we isolated Lactococcus formosensis (Lf) from the liver and MLN of mice which received fecal microbiota transplantation (FMT) from FC patients. The significantly differential metabolites D-ribose was also up-regulated in Lf supernatant. Oral administration of D-ribose inhibited the expression of PAR2-p38 and contributed to constipation. What’s more, in senna leaf-induced diarrhea model, D-ribose down-regulated the expression of PAR2-p38 and performed a distinct anti-diarrhea effect.
Conclusion: D-ribose may cause disorder of gastrointestinal motility and contribute to the development of FC. Our study illustrated the involvement of D-ribose in FC, which provided a new target for the understanding and treating of FC.
PP-01-260
Clinical characteristics and outcomes of immunecheckpoint inhibitor related colitis: a single center retrospective cohort study
Jing Chen and Guanzhou Zhou and Junzhe Chen and Fei Pan
The First Medical Center Of The General Hospital Of The Pla, Beijing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Immune checkpoint inhibitors-related colitis (IRC) is the main reason of discontinue of Immune checkpoint inhibitors (ICIs). The clinical and endoscope characteristics, treatment methods and outcomes of IRC are not well understood. This study describes the above issues and compare the difference between patients having short or long duration from last ICI use to the onset of colitis.
Methods: This study retrospectively enrolled IRC patients from December 2021 to October 2023 diagnosed in one hospital. Based on the duration from last ICI use to the onset of colitis, patients were divided into short group (<2 weeks) and long group (>2 weeks). Clinical and endoscope characteristics were compared.
Results: Totally, 16 males and 4 females were included in study (Figure 1). 11 patients underwent colonoscopy and the Mayo scoring system showed a score of 3 in nine (82.0%) patients, 1 and 2 in one (9.0%) patient respectively. 10 patients received hormone therapy, 1 patient received infliximab treatment, 3 patients received mesalazine combined with probiotics, and 6 patients received symptomatic antidiarrheal treatment. All patients received hormone or infliximab therapy responded well. Patients with short duration from last ICI treatment to the colitis occurrence have lower total protein and albumin level, indicating a worse nourishment.
Conclusion: Clinically, patients with IRC mostly present with diarrhea and they usually have ulcer on the endoscopy with entire colon involvement. Patients with short duration from last ICI treatment to the colitis occurrence indicate a worse nourishment. During clinical treatment, attention should be paid to providing nutritional support.
PP-01-261
Study on the Mechanism of Lactobacillus oris BE7N in Improving Lipid Metabolism Disorders
Yi Chen and Gang Sun
Department Of Gastroenterology And Hepatology, First Medical Center, Chinese Pla General Hospital, China, Beijing, 中国
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The gut microbiota plays a critical role in obesity's pathogenesis, making probiotic therapies a promising future approach. This study investigates the effects and mechanisms of Lactobacillus oris BE7N (LOB7N), isolated from centenarians' fecal samples in Hainan, on lipid metabolism disorders using the db/db obese mouse model.
Materials and Methods: Four-week-old male db/db (C57BL/KsJ) mice were divided into control and treatment groups. The treatment group received LOBE7N bacterial powder by gavage (2x10^8 cfu/mouse/day, 200 μl), while the control group received the equivalent volume of lyophilized powder solution. Glucose tolerance and cold tolerance tests were conducted. Feces were collected for 16S rRNA sequencing. LC-MS/MS-based untargeted metabolomics analyzed secondary metabolites in LOBE7N supernatants with anti-obesity effects.
Results: LOBE7N treatment significantly reduced body weight, epididymal fat, and liver weight in db/db mice. It improved insulin resistance and cold tolerance. Serum TC and LDL-C levels were significantly decreased. HE staining showed it reduced liver fat deposition and improved browning of brown adipose tissue. RT-PCR results indicated it upregulated thermogenic genes and increased fatty acid oxidation genes in skeletal muscle. 16S rRNA sequencing revealed increased cecal microbiota diversity and higher abundance of short-chain fatty acid-producing bacteria. LC-MS/MS-based untargeted metabolomics showed that it secretes lipid-lowering docosapentaenoic acid (DPA) and gut barrier-enhancing indole-3-propionic acid (IPA). In vitro studies indicated these metabolites enhance fatty acid oxidation in muscle cells.
Conclusion: Lactobacillus oris BE7N, may improve lipid metabolism disorders in obese mice by promoting fatty acid oxidation in skeletal muscle through its metabolites, DPA and IPA.
PP-01-262
Animal experimental study on theterahertz spectral characteristics of acute mesenteric ischemia tissue
Yi Chen, Yujia Jing, Jun Chen and Gang Sun
Department Of Gastroenterology And Hepatology, First Medical Center, Chinese Pla General Hospital, Beijing, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Acute mesenteric ischemia (AMI) is associated with high mortality and disability rates, yet early-stage diagnosis remains challenging due to the absence of sensitive and reliable diagnostic methods. Terahertz waves, an emerging non-destructive testing technology, have gained increasing application in the field of biology.
Materials and Methods: In this study, an acute mesenteric ischemia (AMI) model was induced in six Bama miniature pigs through ligation of the mesenteric vessels. Terahertz transmission spectroscopy, spanning the range of 6000-30 cm-1, was conducted at intervals of 0, 30, 60, 120, 240, and 300 minutes post-ligation. To assess mesenteric changes pre- and post-ischemia, gross observation, endoscopic evaluation, histopathological analysis, and terahertz spectral analysis were utilized, with pathological sections serving as the gold standard. The terahertz spectra were then analyzed to assess the ischemic alterations within the mesenteric tissues.
Results: Terahertz transmission spectroscopy was conducted on tissues affected by acute intestinal ischemia; however, no novel characteristic peaks were identified in the resulting spectra. Nonetheless, the absorption coefficient curve displayed an initial increase followed by a subsequent decrease, with the peak absorption coefficient occurring 30 minutes post-ligation. Notably, the most significant change, as indicated by the analysis of the characteristic peak height, was observed in the PO2- symmetric stretch, corresponding to a wavenumber of 1084 cm-1.
Conclusion: Terahertz transmission spectroscopy is useful in the identification of AMI.
PP-01-263
Elobixibat versus Senna in the treatment of chronic constipation in who failed relief with laxatives
Piyapat Chungsamanukool
Bhumibol Adulyadej Hospital, Thailand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
This study was a single-center, randomized, double-blinded controlled study at Bhumibol Adulyadej Hospital from March 1, 2023, until February 29, 2024. Patients who were diagnosed diabetic with functional constipation who have previously failed relief with laxatives. We were randomized to 10 mg elobixibat and 15 mg senna once daily for twelve weeks.
Result: 42 patients were randomized and included in the complete analysis set. In the Elobixibat groups for 12 weeks, the increased frequency of spontaneous bowel movements was significantly higher than baseline 2.67 times per week compared with 1.19 times per week in the senna group (P = <0.001) and a significant increase than baseline Bristol stool scale 2.14 compared to the senna group, which exhibited a Bristol stool scale 1.29 (P = 0.001). The levels of HDL were significantly increased after administration of elobixibat than senna (8.90 mg/dL and -3.57 mg/dL, respectively; P =0.02)
PP-01-264
Accuracy Systemic Inflammation Index, Systemic Inflammatory cell ratios, CEA Distinguishing Malignant or Benign in Colonoscopy
Nelila Fitriani
Sriwijaya University, Palembang, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to evaluate the accuracy of the Systemic Inflammation Index (SII), Systemic Inflammatory Cell Ratios (SICR), and Carcinoembryonic Antigen (CEA) in distinguishing malignant colorectal cancer (CRC) from benign lesions in patients undergoing diagnostic colonoscopy.
Materials and Methods: A retrospective analysis was conducted on 141 patients who underwent diagnostic colonoscopy at Mohammad Hoesin General Hospital from January 2024 to juni 2024. Patients were categorized into two groups based on histopathological findings: malignant (n=71) and benign (n=70) lesions. Serum levels of SII, SICR, and CEA were measured pre-procedure. Statistical analysis, including receiver operating characteristic (ROC) curves, was performed to determine the diagnostic performance of each biomarker.
Results: The mean SII, SICR, and CEA levels were significantly higher in the malignant group compared to the benign group (p<0.001). ROC analysis showed that SII had an area under the curve (AUC) of 0.76, SICR had an AUC of 0.54, and CEA had an AUC of 0.84.
Conclusion: CEA remains a robust biomarker for distinguishing malignant from benign colorectal lesions. Further prospective studies are warranted to validate these findings and refine diagnostic algorithms for CRC.
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Gut barrier function deteriorates with age, which plays a key role in intestinal permeability and protecting gut health.Excess emulsifier P80 intake can disrupt the intestinal barrier, negatively affecting host health.We aimed to investigate the effects of P80 intake on intestinal aging of senescence-accelerated mice.
Materials and Methods: Senescence-accelerated mouse prone-8 mice (SAMP8) mice were randomly divided into two groups, one group was given PBS as the Control group, and the other group was given P80 as the experimental group for three months.The senescence-associated secretory phenotype(SASP), mucosal barrier function, and oxidative stress molecules were assessed. RNA sequencing analysis of the small intestine in the Control group and the P80 group revealed the activation of peroxisome proliferator-activated receptor α-fatty acid-binding protein1(PPARa-FABP1)axis, and further vitro experimental verification.
Results: Emulsifier P80 intake promotes intestinal damage and accelerates intestinal aging in elderly SAMP8 mice. Furthermore, the negative effects of P80 can disrupt intestinal barrier function in aging mice and induce more senescence-associated secretory phenotype. Mechanistically, through RNA sequencing, we found that long-term intake of P80 activates the intestinal PPARa-FABP1 axis, promoting the absorption of fatty acids in the intestine, and accelerating intestinal aging by increasing oxidative stress products. Interestingly, in vitro experiments have demonstrated that P80 promotes d-galactose-induced senescence in Caco-2 cells and enhances lipid accumulation through PPAR signaling pathway, while the PPARα antagonist GW6471 could block this effect and retard senescence.
Conclusion: Emulsifier P80 Intake increases oxidative stress and accelerates intestinal aging in SAMP8 mice through inducing intestinal fatty acid uptake and lipotoxicity.
PP-01-266
CT early signs (pancreatic atrophy/fat deposition) of pancreatic cancer on pre-diagnositic Images.
Noriko Ishigaki
Saiseikai Yamaguchi General Hospital, Yamaguchi-city, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Pancreatic cancer (PC) is primarily diagnosed using CT scans, but often, PC is already advanced at the time of diagnosis. It has been reported that imaging findings such as pancreatic atrophy/fat deposition (CT early signs) can be seen in early-stage PC.The aim of this study is to assess CT early signs in patients who were diagnosed with PC based on pre-diagnostic images.
Materials and Methods: This is a retrospective observational study in our hospital. Of 132 PC patients between 2019 and 2023, we reviewed 50 patients (33 males, median age 75 [range 47-91] ) who had undergone pre-diagnostic CT scans. The primary outcome is the presence of abnormal findings of the pancreas in previous CT scans. The secondary outcomes are the changes in these findings over time.
Results: Among 50 patients with pre-diagnostic CT (median 2 [1-13] times, conducted 3 months to 20 years before diagnosis), 39 patients (78%) had abnormal findings. These abnormal findings included pancreatic atrophy (n=25), fat deposition (n=21), duct ectasia (n=9), and cysts (n=7) (some patients had multiple abnormalities). Of the 39 patients with previous abnormal findings, 20 of 25 (80%) who had multiple CT scans showed changes over time.
Conclusion: Pancreatic atrophy/fat deposition were observed more frequently than duct ectasia or cysts in the present study. Furthermore, these findings were often seen to progress over time. CT early signs may trigger the detection of PC, and we should pay attention to these findings as well.
PP-01-267
Human leukocyte an tigen DQ 2 and DQ 8 expression among renal transplanted patients chronic diarrhea
Raja Taha Yaseen Khan and Abbas Ali Tasneem and Nasir Hassan Luck
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to determine the frequency of Human Leukocyte Antigen (HLA) DQ2 and DQ8 expression among renal transplant patients with DVA and negative celiac serology.
Material and Methods: A cross-sectional study was conducted at the Sindh Institute of Urology and Transplantation (SIUT) from January to June 2021. A total of 61 renal transplant patients aged 18-60 years with DVA and negative celiac serology were included. Data collection involved detailed patient history, laboratory investigations, and HLA DQ typing. Statistical analysis was performed using SPSS version 25.0.
Results: Among the 61 patients, 41 were males (68.3%) and 20 were females (31.7%), with a mean age of 33.8 ± 6.3 years. The predominant symptom was diarrhea (91.7%). HLA typing indicated that 56 patients (91.8%) were diagnosed with SNCD: 54 patients (90%) expressed HLA-DQ2, and 37 patients (61.7%) expressed HLA-DQ8, with 31 patients (51.7%) co-expressing both alleles. Significant associations were found between HLA-DQ2/DQ8 positivity and male gender, microcytic anemia, chronic diarrhea, and weight loss.
Conclusion: This study demonstrates a high prevalence of HLA-DQ2 and DQ8 among renal transplant patients with DVA and negative celiac serology, suggesting a genetic predisposition to celiac disease despite negative serological markers.
PP-01-268
Cytomegalovirus associated colitis as a cause of lower gastrointestinal bleeding in kidney transplant recipients
Raja Taha Yaseen Khan, Vijesh Kumar, Nasir Hassan Luck and Abbas Ali Tasneem
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: The aim of this study was to determine the frequency of cytomegalovirus associated colitis in Kidney transplant Recipients (KTRs) presenting with lower gastrointestinal bleeding.
Materials and Methods: This cross-sectional study was conducted at the department of hepatogastroenterology ,Sindh Institute of Urology and Transplantation from January 2021 to December 2021.All the KTRs (6 months after the transplantation) of either gender and aged between 18 to 65 years, presenting with lower gastrointestinal (GI) bleeding were enrolled in the study. Colonic biopsies were reviewed by consultant histopathologist for the features of CMV infection.
Results: A total of 95 renal transplant recipients of either gender or age above 18 to 65 years with the presenting with lower GI bleeding were included in the study. Most common presenting complaint was fresh bleeding per rectum which was observed in 73(76.8%). The most common finding observed on colonoscopy in KTRs with bleeding per rectum was colonic ulcers and erosions noted in 41(43.1%) and 36(37.9%) patients respectively. On histopathology, CMV Colitis was noted in 21(22.1%) patients. On comparison of different baseline variables, the presence of fresh bleeding per rectum and presence of both ulcers and erosions on colonoscopy were the factors significantly associated with CMV colitis in KTRs.
Conclusion: CMV associated colitis was noted in 22.1% of our KTRs presenting with lower gastrointestinal bleeding. However, in our study, the levels of viremia had no significant association with the presence of CMV associated colitis.
PP-01-269
Differential diagnosis based on clinical symptoms and endoscopic findings in symptomatic terminal ileitis or ulcers
Woojin Kim, Cheolwoong Choi and Subum Park
Pusan National University Yangsan Hospital, Yangsan, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: The accurate identification and management of symptomatic terminal ileitis or ulcers (STIUs) remain challenging. STIUs is a common finding on ileocolonoscopy and may be related with a wide variety of diseases. The aim was the etiology and discrimination of various disease in STIUs.
Materials and Methods: This is a retrospective study including patients with diagnosis of STIUs without colonic abnormalities on ileocolonoscopy for various symptoms in a tertiary center between January 2018 and October 2023. Data of demographics, clinical manifestations and endoscopic findings were collected.
Results: 110 of 224 patients who had ITIUs on ileocolonoscopy have symptoms. Among 110, 71 had specific etiologies on initial testing and after 6 months follow-up. Definitive diagnosis was ascertained in Crohn’s disease (CD): 30, Behçet’s disease (BD): 20, infectious enteritis: 10, drug-induced ulcers: 5, intestinal tuberculosis (ITB): 5, lymphoma: 1, and 40 patients had nonspecific ulcers. After 1 year treatment, symptomatic and endoscopic resolution were noted in 7/30 patients with CD and 10/20 patients with BD, respectively. Of the 40 patients initially diagnosed with nonspecific ulcers with persistent symptoms, two were eventually diagnosed with CD.
Conclusions: Disease was diagnosed in more than half of STIUs patients, with Crohn’s disease and Behçet’s disease being the most common disease in this study. Combining clinical characteristics such as oral ulcer, genital ulcer, hemoglobin and CRP with endoscopic findings can be helpful in differential diagnosis of STIUs. Therefore, accurate diagnosis and appropriate treatment through follow-up are important because of STIUs is diagnosed in half of symptomatic patients
PP-01-270
Causal Effects of Gut Microbiota on Viral infection: Insights from Genetic
Shuang Ma
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Gut microbiota has been increasingly linked to the host susceptibility of virus infection, but the causal association remains unclear.
Methods: We conducted a two-sample Mendelian randomization (MR) analysis to assess the potential causal effect of gut microbiota on viral susceptibility. Summary-level data for gut microbiota and seven viral infection outcomes were obtained from the genome-wide association study (GWAS) of the MiBioGen and FinnGen Consortium, respectively. The inverse-variance weighted (IVW) method was employed as the primary approach. Additionally, multiple sensitivity analyses were carried out to verify the robustness of the results.
Results: After adjusting for multiple tests, we observed that the genetically predicted genera Lachnospiraceae ND3007 group (OR: 1.23, 95% CI: 1.05−1.43, q = 0.029) and Marvinbryantia (OR: 1.22, 95% CI: 1.09−1.36, q = 0.005) were causally associated with an increased risk of viral gastroenteritis, while the genus Parabacteroides (OR: 0.81, 95% CI: 0.71−0.92, q = 0.007) was associated with a decreased risk of viral gastroenteritis. Additionally, Genus Methanobrevibacter posed a protective effect against COVID-19 (OR: 0.96, 95% CI: 0.94−0.99, q = 0.029), but conversely increased the risk of herpes simplex virus (OR: 1.16, 95% CI: 1.04−1.29, q = 0.029). Sensitivity analyses confirmed the robustness of the IVW results.
Conclusion: These findings indicate that specific gut microbiota exerts causal effects on viral infection, highlighting the potential for leveraging specific gut microbiota or their metabolites to inform strategies for preventing and managing viral infections.
PP-01-271
Lactobacillus johnsonii alleviates NSAID-induced enteropathy by inhibiting GSDMD-mediated pyroptosis in intestinal epithelial cells
Shuang Ma, Lanping Zhu and Xin Chen
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Gut microbiota dysbiosis is implicated in the pathogenesis of non-steroidal anti-inflammatory drugs (NSAIDs)-related enteropathy. Lactobacillus johnsonii (L. johnsonii), a probiotic, is recognized for its ability to modulate immune microenvironments, regulate gut microbiota, and repair intestinal barriers. This study aims to explore the preventive and therapeutic effects of L. johnsonii on NSAIDs-induced enteropathy and its underlying mechanisms.
Materials and Methods: Small intestinal injury in mice was established by indomethacin and gavaged with L. johnsonii or its supernatant. The effect of L. johnsonii in relieving small intestinal injury were assessed. The underlying mechanisms were evaluated both in vivo and in vitro.
Results: L.johnsonii effectively alleviated indomethacin-induced small intestinal injury in mice. Mechanistically, RNA sequencing revealed downregulation of gene expression associated with the inflammasome complex and pyroptosis following L. johnsonii treatment. Additionally, L. johnsonii inhibited intestinal inflammation, restored the damaged intestinal mucosal barrier, and decreased the expression of pyroptosis-related proteins (NLRP3, Caspase1, GSDMD) in the small intestinal tissues of mice. L. johnsonii supernatant was further demonstrated to suppress pyroptosis in intestinal epithelial cells of mice in vitro, increasing cell viability and reducing LDH release as well as inflammatory cytokines IL-1β and IL-18.
Conclusion: L.johnsonii ameliorates NSAIDs-induced enteropathy by inhibiting pyroptosis in intestinal epithelial cells via NLRP3/Caspase1/GSDMD signaling pathway. This study provides a theoretical basis for the use of probiotics in preventing and treating NSAIDs-related enteropathy, proposing new therapeutic strategies for clinical treatment.
PP-01-272
Chronic Diarrhea Caused by Helminthiasis: a Case Report
Maybelline Maybelline and Benny Budiman
Mitra Medika Hospital, Pontianak, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Helminthiasis poses significant public health problems in developing countries. Consuming raw vegetables without proper washing is one of the main routes of intestinal parasite acquisition.
Case Illustration: A 47-year-old female presented with diarrhea and abdominal pain since two weeks ago. Stools were watery, foul-smelling, with mucus, and without blood. Stool frequency was approximately 4-5 times per day. There was neither fever, weight loss, perianal itching, vomiting blood, nor dark stool. No history of allergy or asthma was reported. She had a history of consuming fresh, raw vegetables frequently. She was given ciprofloxacin 500 mg twice daily one week after the onset of diarrhea, but symptoms persisted. At admission, a complete blood count revealed leukocytosis (16.450 mm3) with monocytosis and no eosinophil elevation. No worm egg or amoeba was found on the stool examination. She was diagnosed with chronic diarrhea caused by IBD, with helminthiasis as the differential diagnosis. A colonoscopic examination demonstrated the presence of whitish parasites with a whip-like appearance on the caecum and erosion of the ascending colon. Chronic diarrhea caused by helminthiasis was diagnosed with a suspicion of Trichuris trichiura infection. She was treated with mebendazole 500 mg once daily for three days, and her symptoms improved.
Discussion: Helminthiasis must be considered as one of the causes of chronic diarrhea. Negative worm egg found in stool examination and no eosinophilia should not rule out the possibility of helminthiasis. Antihelminthic agents, like benzimidazole-agent, are effective in treating helminthiasis.
Nutrition Care after Hospital Discharge in Singapore: Evidence-Based Best-Practice Recommendations
Doris Ng1, Frederick Koh2, Hazel Yong3, Terence Cheong1, Min Chue Koy2, Fung Joon Foo2 and Samuel Chew4
1Tan Tock Seng Hospital, Singapore, Singapore;2SengKang General Hospital, Singapore, Singapore;3Khoo Teck Puat Hospital, Singapore, Singapore;4Changi General Hospital, Singapore, Singapore
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Hospitalised patients are at risk of compromised nutritional status and predisposed to further deterioration after discharge, leading to poor clinical outcomes, high healthcare costs, and poor quality of life. We aimed to provide evidence-based best-practice recommendations to address this gap in nutrition care.
Materials and Methods: A multidisciplinary healthcare professionals (HCPs) panel from the Society of Parenteral and Enteral Nutrition (Singapore) conducted PubMed searches and summarised current literature on best practices on nutrition care after hospitalization. A national survey of HCPs from Singapore’s healthcare institutions was conducted to gain insights into existing practices and identify gaps. The panel reviewed 242 responses received and then put forth ten best-practice recommendations.
Results: We advocate screening all patients using a validated tool with a disease activity/burden component, an easily accessible dietitian referral pathway for patients at risk of malnutrition, and an individualised nutrition care plan formulated and delivered using multidisciplinary team approaches for patients at risk or with malnutrition. A comprehensive team would include dietitians, physicians, nurses, physiotherapists, speech therapists, and medical social workers working together towards a common goal. Information on the importance of good nutrition and health and how it can be achieved should be provided to patients and their caregivers before and after hospital discharge.
Conclusion: Nutrition screening during hospitalization and at discharge are essential to identify patients who are at risk or with malnutrition prior to discharge. With the above recommendations, we seek to improve upon the current nutrition care processes at discharge for healthcare institutions in Singapore.
PP-01-274
Effect of autonomic dysfunction on hemodynamic instability during per-oral endoscopic myotomy in achalasia patients
Min-Jae Kim, Young Hoon Youn and Hyojin Park
Yousei University College Of Medicine, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Per-oral endoscopic myotomy (POEM) is a common procedure for esophageal dysphagia. CO2 insufflation during POEM helps create a submucosal tunnel but may lead to complications such as capnoperitoneum and hemodynamic instability. Patients with autonomic dysfunction might experience more severe instability during POEM, but the impact is uncertain. This study aims to investigate the effect of autonomic dysfunction on intraoperative hemodynamic instability during POEM in patients with esophageal dysphagia.
Materials and Methods: This single-center prospective observational study began in April 2023. Participants were adult patients aged 19 years and older undergoing POEM. Exclusion criteria were pre-existing hemodynamic instability, minors, and lack of consent. All participants underwent an HRV test for autonomic dysfunction assessment the morning before POEM. The primary outcome was the wobble of systolic arterial pressure (SAP) during POEM.
Results: By June 2024, 20 achalasia patients were enrolled. HRV tests confirmed autonomic dysfunction in 8 patients (13 males [65%]). The mean age was 40.2 years (range, 21–72). The autonomic dysfunction group showed a higher wobble of SAP (11.1 vs 12.6, mean), but the difference was not statistically significant (p=0.417). Intraoperative factors such as remifentanil infusion rate adjustments and the use of vasoactive medications (phenylephrine, ephedrine, nicardipine) were higher in the autonomic dysfunction group, with significant differences only in ephedrine use (p=0.043).
Conclusion: Patients with achalasia and autonomic dysfunction may experience hemodynamic instability during POEM, necessitating more vigilant intraoperative monitoring.
PP-01-275
Comparison of Etiologies and Outcomes of Lower Gastrointestinal Bleeding Occurring in Outpatients and Inpatients
Watsamon Parkpian and Supot Pongprasobchai
Department of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to investigate the differences between the etiologies and outcomes of inpatients vs outpatient LGIB. Methods: A retrospective cohort study of patients with LGIB who underwent colonoscopy at Siriraj Hospital from 2020 to 2021 was performed. We included outpatients and inpatient at a ratio of 3:1 and compared the demographics, presentations, etiologies, treatment, and outcomes.
Results: There were 190 LGIB patients, 126 outpatient and 64 inpatient. The mean ages and gender were similar between both groups. Severe hematochezia was more common in inpatient than outpatient LGIB (34% vs 17%, p=0.06). The leading etiologies of outpatient LGIB were diverticular bleeding (40%), colonic cancer (7%), radiation enteritis (6%) and angioectasia (5%), while those of inpatient LGIB were acute hemorrhagic rectal ulcer syndrome (AHRUS, 17%), CMV colitis (13%), diverticular bleeding (11%), colonic cancer (9%) and ischemic colitis (5%). AHRUS was significantly more common in inpatient than outpatient LGIB (17% vs. 2%, p<0.001) while diverticular bleeding was more common in outpatient than inpatient LGIB (40% vs 11%, p<0.001). Inpatients LGIB had longer median length of stay (LOS, 33 days vs 6 days, p<0.001), higher rebleeding rate (34% vs 9%, p<0.001), and higher 30-day mortality (25% vs 3%, p<0.001). Independent factors associated with mortality were rebleeding (odds ratio [OR] 130), underlying cirrhosis (OR 28.8), initial hypotension (OR 9.6), blood transfusion (OR 1.1) and inpatient LGIB (OR 5.4).
Conclusion: Inpatient LGIB had poorer outcomes than outpatient LGIB. The leading etiology of inpatient LGIB was AHRUS, while that of outpatient was diverticular bleeding.
PP-01-276
Psychological assessment of subjects with positive Fecal Immunochemical test in Phu Tho province - Vietnam
Tiến sĩ Phu Pham Quang1, Thuan Nghiem Duc2, Tuan Le Quoc3, Thuong Nguyen Hoai1, Nguyen NGuyen Canh2, Kim Nguyen Kim1, Lai Nguyen Van1, Linh Nguyen Thuy1, Chi Dinh Minh1, Huong Nguyen Thi Thanh1 and Huong Huynh Thi Thu1
1Military Hospital 103, Hanoi, Vietnam;2Vietnam Military Medical University, Hanoi, Vietnam;3Thanh Ba District Medical Center, Phu Tho, Vietnam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Understanding the relationship between the patient's psychology with Fecal Immunochemical Test (FIT) positive for the decision to make a decision to screen the cause.
Materials and Methods: Research describing cross -section over 216 patients from 40 to 80, with FIT positive after meeting the elimination standard, the time from July 2023 to May 2024. Psychiatric assessment of patient was conducted results through answering the test of anxiety, based on the scale Inventory-Form Y1 (stai-y1).
Results: The male/female ratio is approximately 1.3/1. The average income component (5-10 million VND/month) accounts for the highest proportion (47.2%), of which the intellectual labor and manual labor account for the approximate proportion (46.1%, 53.9%). Most have no knowledge of both CRC and FIT (48.1%). Most of the endoscopic subjects find the cause of gastrointestinal pathology (81.5%). The percentage of anxiety accounts for 88%, with a low and medium level (35.6%, 39.8%). The level of anxiety according to the Stai - Y1 ladder has no association with the re -examination plan (x2 = 0.125, p = 0.974), but there is a relationship with laparoscopy to find the cause (x2 = 0.9705 and p = 0.021). In addition, there is a relationship between the endoscopic resolution and the education level (x2 = 0.877, p = 0.001) and with the groups of understanding CRC-FOBT (x2 = 0.9609, p = 0.022).
Conclusion: There is a relationship between the patient's psychology with FIT positive, as well as the level of education, understanding of the knowledge of CRC - FIT
PP-01-278
A Case of Pseudo-Pseudo Meigs Syndrome (PPMS) due to SLE with Massive Ascites
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: PPMS, characterized by ascites, pleural effusion, and elevated CA125, is a rare manifestation of lupus peritonitis first described in 2005. We report a case of PPMS that was difficult to diagnose.
Case Description: A 42-year-old woman presented with chronic diarrhea, generalized edema, and abdominal distension. She had a history of necrotizing lymphadenitis and irritable bowel syndrome. She was referred to our hospital due to abdominal distension and generalized edema. Initial examination revealed hypoalbuminemia, generalized edema, and weight gain. Contrast-enhanced CT showed ascites without liver cirrhosis, portal vein thrombosis, inferior vena cava obstruction, or intestinal edema. Gastrointestinal endoscopies and protein-losing enteropathy scintigraphy were normal. Cardiac disease and nephrotic syndrome were ruled out. During the examination and follow-up, it showed worsening hypoalbuminemia, increased ascites, and new right pleural effusion. Ascites analysis revealed low SAAG, with negative cultures. Elevated serum CA125 suggested possible peritoneal carcinomatosis, but multiple cytologies were negative. Immunological tests performed due to persistent low grade fever showed elevated anti-nuclear and anti-ds-DNA antibodies and low complement levels. Re-evaluation identified Raynaud's phenomenon and chilblain-like lesions. A skin biopsy indicated lupus with a positive lupus band test, diagnosed PPMS secondary to SLE. The patient was treated with systemic and intraperitoneal glucocorticoids and immunosuppressants, resulting in a gradual reduction of ascites, improved albumin levels, and normalized CA125.
Discussion: This case highlights the diagnostic challenges of PPMS in the absence of a prior SLE diagnosis.PPMS should be suspected in the presence of unexplained ascites effusion and the above signs.
PP-01-279
A Rare Case: Chilaidity Syndrome, Uncommon Abdominal Pain in Right Upper Quadran
Finly Septianto1, Titong Sugihartono1, Budi Widodo1, Herry Purbayu1, Tri Asih Imroati2 and Annisa Zahra Mufida1,2
1RSUD dr Soetomo, Surabaya, Indonesia;2Airlangga Teaching Hospital, Surabaya, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Chilaidity Syndrome is a rare condition with unique gastrointestinal symptoms due to the interposition of the colon between the liver and diaphragm caused by decreased chronic motility. This condition can lead to various complications. Understanding the etiology, diagnostic method and treatment option for Chilaidity syndrome is important.
Case Report: A 70 years male patient presented with black starry stool and right upper quadrant abdomen pain. The pain appears when sleeping and improve when awake. Because of his complaints, patient was planning for colonoscopy. The X-rays revealed gas shadow of the intestine in the inferior of the right hemidiaphragm that confirm of Chilaidity syndrome. The colonoscopy revealed a mass on ascendent colon suspicious for adenocarcinoma well differentiated (T2N0Mx), Dukes stage A, MAC stage B1 indicated for surgery. The laboratories revealed slight anemia while the other are normal. Ultrasound abdomen revealed Benign Prostate Hyperplasia and minimal ascites in the M’c Burney while CT-Scan abdomen with contrast perform with no mass shadow results.
Discussion: The Placement of the colon or small intestine between the liver and right diaphragm in the absence of symptoms is known as the Chilaidity sign. Abdominal pain is one of the symptoms associated with the Chilaidity sign, a disorder known as Chilaidity syndrome. Conservative is the best treatment. It is important to consider Chilaidity syndrome presents with predisposing factor such as GIT cancers. Imaging studies, such as X-rays or CT-Scan, play a crucial role for confirm the diagnosis.
PP-01-280
Paraneoplastic pseudoachalasia secondary to peritoneal sarcomatoid mesothelioma
Charlene Tan1, Paolo Francisco Carlos2, Yi Yuan Tan1,3, Kim Wei Lim1,3, Asokkumar Ravishankar1,3 and R Rajesh1,3
1Department of Gastroenterology and Hepatology, Singapore General Hospital;2Institute of Digestive and Liver Diseases, St Luke’s Medical Centre- Global City, Philippines;3DUKE-NUS Graduate Medical School, Singapore
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Paraneoplastic pseudoachalasia is a rare finding that is most commonly associated with gastrointestinal malignancies. We describe an unusual case of paraneoplastic pseudoachalasia associated with peritoneal sarcomatoid mesothelioma.
Case Description: We present a case of a 56 year old male who presented to our emergency department with lower abdominal distension, dysphagia and early satiety. Computed topography of the thorax, abdomen and pelvis showed a distended oesophagus with abrupt change in caliber at the gastroesophageal junction (GEJ), a dilated appendix, as well as peritoneal thickening and caking suspicious for peritoneal disease. Endoscopic evaluation showed narrowing of the GEJ with visible puckering but no signs of underlying mass, stricture or oesophagitis. Multiple biopsies were taken during endoscopic evaluation but histologic examination of the samples was unremarkable. Barium swallow showed findings consistent with achalasia. The stenosis was treated by dilatation and ultrasound-guided peritoneal biopsy was performed, with histology consistent with sarcomatoid mesothelioma. The patient was subsequently diagnosed with metastatic peritoneal sarcomatoid mesothelioma with paraneoplastic pseudochalasia.
Discussion: Pseudoachalasia can present similarly to achalasia and a high index of suspicion is needed especially in patients of advanced age, with a short onset of symptoms and significant loss of weight. Further investigations should be pursued to exclude an underlying malignancy. When there is no direct infiltration or compression of the GEJ, paraneoplastic pseudo-achalasia from a distant source should be considered.
PP-01-281
Differences in Neutrophil-Lymphocyte Ratio in Dyspepsia Syndrome Patients with and without Peptic Ulcer
Pebria Suryani Valentin1, Bradley Waleleng2, Luciana Rotty2, Fandy Gosal2, Jeanne Winarta2 and Andrew Waleleng2
1Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University, Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;2Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University, Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Peptic ulcer causes long-term inflammation resulting in changes in the stomach and duodenum movement and sensitivity, becoming the most common cause of dyspepsia. However, there have not been many studies on dyspeptic patients with peptic ulcers. A recent study by Coşgun and Aras in 2023 mentioned that patients with peptic ulcers have higher NLR values compared to patients without peptic ulcers. Neutrophil-lymphocyte ratio (NLR) is a cost-effective and efficient biomarker associated with several inflammatory disorders. This study aims to analyze the difference in NLR values in dyspepsia patients with and without peptic ulcers at Prof. R.D. Kandou Hospital Manado in the period January-April 2024.
Materials and Methods: A cross-sectional observational study was conducted on patients diagnosed with dyspepsia and underwent endoscopy at RSUP Prof. R. D. Kandou Manado from January to April 2024. Data were not normally distributed so associations were analyzed using Mann Whitney U test. The p-value of <0.05 was considered significant.
Results: A total of 53 research subjects were included in this study, most of whom were male (54.7%) with average age of 56.39 ± 12.99 years. Endoscopy showed that 15 patients (28.3%) had peptic ulcers and 38 patients (71.7%) without peptic ulcers. The NLR value was significantly higher in patients with peptic ulcers (5.35 ± 7.67) compared to patients without peptic ulcers (2.82 ± 3.11, p value=0.037).
Conclusion: Patients with peptic ulcers have higher NLR values compared to patients without peptic ulcers.
PP-01-282
Investigation of the Factors Necessary to Achieve Diversity and Inclusion among Japanese GI Physicians
Takanori Yamada
Hamamatsu University School Of Medicine, Hamamatsu, Japan
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Diversity has revealed that even though a variety of skills and ideas exist, a psychologically safety is necessary to make the most of them (inclusion). We attempted to determine what factors are necessary for the practice of gastroenterology to be inclusive of all individuals and to achieve both productivity and personal well-being.
Materials and Methods: Questionnaires were administered on physician profile, job satisfaction, personal satisfaction, Utrecht Work Engagement Scale, and AC Edmondson psychological safety score.
Results: Forty-one GI physicians from five institutions responded to the survey. Six residents, 16 consultants, 12 instructors, and 7 others, 43.5±39.2 hours/month of overtime, 4.9±1.8 days/month of non-work days, 13.4±10.8 hours/week of housework/child care, 33.0±10.0/54 points on the engagement scale, 37.5±7.0/49 points on the psychological safety score The results were. Team productivity was correlated with job satisfaction (ρ=0.481) and holiday satisfaction (ρ=0.425), and own productivity was correlated with evaluation of skills (ρ=0.441) among psychological safety items, but not with the total psychological safety score (ρ=0.134). Evaluation of skills correlated more strongly with total engagement (ρ=0.461), of which vitality (ρ=0.496) and enthusiasm (ρ=0.537) that the job provided. Furthermore, team productivity, job satisfaction showed stronger correlations with holiday satisfaction (ρ=0.687), personal satisfaction (ρ=0.672), number of days away from work (ρ=0.495), total psychological safety (ρ=0.363), and among these, ease of proposing difficulties and difficult problems (ρ=0.547).
Conclusion: It was suggested that evaluation of skills, ease of proposals, and enrichment of holidays and personal life may contribute to improving GI physicians' job satisfaction and performance
PP-01-283
Gastirc microbiota of malignant transformation of gastric proliferative polyps
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Object: To explore differences of microflora between gastric gastric proliferative and high-grade intraepithelial neoplasia of gastric hyperplastic polyps, and explore the further pathogenesis.
Method: Patients with gastric hyperplastic polyps and gastric hyperplastic polyps with high-grade intraepithelial neoplasia (high-grade dysplasia and carcinogenesis) diagnosed pathologically were selected and matched according to age and sex, with 10 patients in each group. Paraffin embedded specimens of their tissues were collected. 16SrRNA high-throughput sequencing and bioinformatics were used to analyze the diversity and species composition of gastric hyperplastic polyp in two groups of patients.
Results: There was no significant difference in the α-diversity of the bacterial microbiota between the two groups, but there was a difference in β-diversity. The bacterial abundance measurement showed that Shewanella(ASV) was higher in patients with high-grade intraepithelial neoplasia of gastric hyperplastic polyps than in patients with simple gastric hyperplastic polyps, while ASV of Sphingomonadaceae and Flavisolibacte was decreased (both P<0.05).
LEfSe analysis revealed that Acinetobacter, Shewanella and Ramlibacter were enriched in patients with high-grade intraepithelial neoplasia of gastric hyperplastic polyps, while Rubrobacte, Sphingomonadaceae-unclassified and Lysinibacillus were enriched in the patients with simple gastric hyperplastic polyps.
Conclusion: Patients with gastric hyperplastic polyps with high-grade intraepithelial neoplasia and patients with gastric hyperplastic polyps alone have different microflora characteristics, the specific mechanism of which is still unclear and needs to be further revealed.
PP-01-284
Diagnostic Value of Spot Urine Na/K Ratio Compared to 24-hour Urine Sodium in cirrhotic patients
Sukanta Chandra Das1 and Naymul Hasan2
1Kurmitola General Hospital, Dhaka, Bangladesh;2Shaheed ziaur Rahma medical college, Bogra, Bangladesh
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Ascites is a major complication of liver cirrhosis that carries a poor prognosis. Monitoring of diuretic response can be achieved by measurement of 24 hour urinary sodium. The “spot” urine sodium/potassium (UNa/K) ratio can be a convenient tool to identify 24 hour urinary sodium excretion.
Materials and Methods: This was a cross-sectional study to evaluate natriuresis in patients with decompensated liver cirrhosis with ascites admitted in Banghabandhu sheikh mujib medical university. 100 patients were included in this study.The Na/Ku ratio was calculated based on the values of sodium and potassium in “spot” urine sample which was obtained before or after completion of 24-hr urine collection. Instructions was given to assure completeness of urine collection.The correlation between the 24hr-UNa and spot UNa/K ratio was evaluated by the Pearson’s correlation coefficient. Diagnostic accuracy of the spot UNa/K ratio was analysed by estimating the area under the receiver operating characteristics curve (AUROC) and by calculating accuracy, sensitivity, specificity, positive and negative predictive value.
Results: Spot urine Na/K ratio has adequate sensitivity and accuracy for assessment of natriuresis compared with 24 hr urinary sodium in cirrhotic patients with ascites at cut off value 1.
Conclusion: So, spot UNa/K ratio is an accurate, cost-effective and convenient method for replacing 24-hr UNa. But large multicentred studies are needed to recommend this test as a routine.
PP-01-285
Non-invasively Differentiate Non-alcoholic Steatohepatitis by Visualizing Hepatic Integrin αvβ3 Expression with Molecular Imaging
Ling Wu, Chenyi Rao, Xiaoquan Huang and Shiyao Chen and Feng Li
Zhongshan Hospital, Fudan University, Shanghai, China
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Currently, Early identification of patients with metabolism associated steatohepatitis (MASH) is essential to prevent progression and outcome of patients. This study aimed to develop an integrin αvβ3-targeted molecular imaging modality to grade and stage MASH non-invasively.
Materials and Methods: Integrin αvβ3 expression was determined in LO2 hepatocytes cultured with palmitate acid and oleic acid (FFA). Hepatic integrin αvβ3 expression was assessed in rabbits an d rats fed with a high-fat diet (HFD) or high-fat high-carbohydrate diet (HFCD). An imaging construct containing cyclic arginine-glycine-aspartic acid (cRGD) synthesized and labeled with gadolinium (Gd) was used as a contrast agent, and magnetic resonance imaging (MRI) was performed in mice fed the HFCD.
Results: Integrin αvβ3 was markedly expressed by FFA-cultured hepatocytes but not by normal hepatocytes. In both HFD-fed rabbits and rats, hepatic integrin αvβ3 expression was significantly increased when simple fatty liver (FL) progressed to steatohepatitis. The distribution of integrin αvβ3 in the livers with MASH overlapped with albumin-stained hepatocytes. Compared to mice with simple FL, the relative liver MRI-T1 signal value at 60 minutes post-injection of Gd-labeled cRGD was significantly increased in mice with histological steatohepatitis (P < 0.05), and showed a positive correlation with the MAFLD activity score (r = 0.945, P < 0.01).
Conclusion: Hepatic integrin αvβ3 expression was significantly increased during the development progression of MASH, and hepatocytes were primary cells that highly upregulated integrin αvβ3 in MASH livers. Our Gd-labeled cRGD MRI tracer is a promising agent to quantitatively image MASH activity over the whole liver.
PP-01-286
Alcohol-driven gut dysbiosis triggers linoleic acid accumulation and inflammation in rat-model of Alcohol-associated liver disease
Institute of Liver and Biliary Sciences, New Delhi, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To study systemic metabolomic changes induced due to alcohol associated microbiome and healthy microbiome post FMT in Alcohol associated liver disease.
Material and Methods: We utilized humanized rats to assess how altered microbiota and microbiome affects metabolism. Criss-cross FMT involved transplanting healthy-donor microbiota into ALD-rats and ALD-patient microbiota into healthy-rats was performed. Metabolomics and metaproteomic analysis were performed in liver, plasma, intestine, and stool.
Results: Chronic ethanol consumption in Long-Evans rats disrupted metabolic pathways in liver, intestine, stool, and plasma, including butanoate, bile, arachidonic acid, linoleic acid, and microbial pathways. Metaproteome analysis revealed decreased levels of Lactobacillus species (L. delbrueckii and L. plantarum), leading to increased accumulation of linoleic acid in liver and intestine. Elevated linoleic acid levels correlated with inflammation and oxidative stress, accompanied by upregulation of cyp1a1 mRNA expression associated with linoleic and arachidonic acid metabolism.
In rats with alcoholic liver disease (ALD), fecal microbiota transplantation (FMT) from healthy donors restored Lactobacillus species, reduced inflammation, ROS production, and normalized cyp1a1 expression and linoleic acid metabolism. Conversely, transplantation of alcohol-associated microbiota from ALD patients to healthy rats significantly increased cyp1a1 expression, inflammation, ROS production, and linoleic acid metabolism.
Conclusion: Our findings suggest that chronic alcohol consumption reduces beneficial Lactobacillus species in the intestine, leading to linoleic acid accumulation, inflammation, and ROS production. FMT with microbiota capable of metabolizing linoleic acid into anti-inflammatory products restores intestinal health. Targeting linoleic acid metabolism and using Lactobacillus strains as probiotics could potentially offer therapeutic benefits in alcoholic liver disease.
PP-01-287
Not The Usual: A Large Well- Differentiated, Non- AFP Producing HCC with Atypical Imaging Features
Christine Velasquez and Julieta G. Cervantes
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Hepatocellular carcinoma (HCC), the 6th most common cancer presents with varying characteristics that lead to atypical imaging findings and diagnostic challenges. HCC progresses from pre-neoplastic lesions to early HCC, which is well-differentiated. As it grows, it de-differentiates. Well-differentiated HCC over 3 cm is rare and has distinct characteristics compared to typical HCC.
Case Description: A 66-year-old male presented with a three-month history of progressive right upper quadrant pain, easy fatigability, and weight loss. Initial workups revealed elevated liver enzymes, prolonged INR, previous hepatitis B infection, and normal tumor markers. Abdominal ultrasound showed cirrhosis and multiple liver masses with malignant characteristics. PET scan was negative for metastasis. Percutaneous liver biopsy was equivocal, showing atypical malignant cells (CK7+, CK20-). Further diagnostic imaging included elastography (F4) for fibrosis staging and four-phase dynamic CT to describe lesions. LI-RADS system showed arterially enhancing lesions (LR3,LR4,LRM) with no washout. The impression was atypical HCC versus combined HCC-cholangiocarcinoma. MRI of the Liver also showed early enhancing foci without demonstrable washout, confirming the CT findings. Results were indeterminate, hence laparoscopic-guided biopsy was done showing d well-differentiated HCC. The patient was classified as BCLC C (lesion characteristics, Child-Pugh 7B, ECOG1) and started on systemic therapy with Lenvatinib which was tolerated.
Discussion: Due to the complexity of hepatocarcinogenesis, HCC can have atypical imaging findings, making diagnosis difficult. The classical arterial phase enhancement and portal/delayed phase washout are not always seen. Establishing a definitive diagnosis is crucial for management decisions, and a biopsy is performed when imaging modalities are inconclusive.
PP-01-288
Standard-Dose Ursodeoxycholic Acid Improves Liver Function in Chronic Liver Disease: A Randomized, Double-Blind, Placebo-Controlled Trial
Young Chang and Yong Kyun Cho and Young Seok Kim and Sung-Eun Kim and Gab Jin Cheon and Ji Hoon Kim and Hyun Yang and Won Kim and Sang Bong Ahn and Eileen L Yoon and Jae-Youn Cheong and Jin-Woo Lee and Moon Young Kim and Hyung Joon Kim and Sae Hwan Lee and Eun Young Cho and Na Ryung Choi and Hye Won Lee and Kang Mo Kim and Won Hyeok Choe and Jung Min Yu and Sun Young Lee and Hye Jung Lee and Su Young Kim and Jae Young Jang
Soonchunhyang University, Seoul, South Korea
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: This study assessed the efficacy and safety of ursodeoxycholic acid (UDCA), administered at 100 mg three times a day, compared to placebo in patients with chronic liver disease.
Methods: We conducted a multicenter, double-blind, placebo-controlled, randomized phase IV clinical trial in academic hospitals in South Korea. Patients with chronic liver disease and abnormal serum ALT levels in at least two consecutive results prior to screening, persisting for at least six months, were randomly assigned to the treatment or placebo group. Patients received UDCA 100 mg or placebo three times a day for 8 weeks. The primary endpoint was the mean relative change in ALT concentrations from baseline. Post-treatment assessments included changes in fibrosis measured using FibroTest and drug-related adverse events.
Results: Between February 2023 and March 2024, 263 patients were analyzed: 132 in the UDCA group and 131 in the placebo group. At week 8, UDCA-treated patients showed a significantly greater reduction in serum ALT from baseline compared to the placebo group (-14.70 U/L vs. -5.51 U/L; p=0.0104). The percent change was also greater in the UDCA group (-17.43% vs. -5.82%; p=0.0112). ALT normalization was higher in the UDCA group (26.52% vs. 13.08%; p=0.0050). Fibrosis reduction was greater in the UDCA group (-0.03 vs. -0.00; p=0.0157). Adverse event frequencies were similar, with no serious adverse events in the UDCA group.
Conclusions: UDCA 100 mg three times a day for 8 weeks demonstrated superior therapeutic efficacy and a favorable safety profile compared to placebo in patients with chronic liver disease.
PP-01-289
A 19-Year Old Female Patient with Congenital Absence of the Portal Vein: A Rare Case
Cecilia Oktaria Permatadewi1, Achmad Fathi Fuadi2, Erick Prabowo2, Antonius Gunawan Santoso3, Didik Indiarso1, Agung Prasetyo1, Hesti Triwahyu Hutami1, Hirlan Hirlan1 and Hery Djagat Purnomo1
1Division of Gastroentero- Hepatology, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia;2Department of Digestive Surgery, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia;3Department of Radiology, Faculty of Medicine Diponegoro University, Kariadi Hospital, Semarang, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: Congenital absence of the portal vein (CAPV) is a rare congenital anomaly involving the absence of the portal vein. Understanding CAPV's clinical presentation and diagnostic approach is crucial for appropriate management.
Case Report: We report a case of a 19-year-old girl who presented with recurrent haematemesis and melena since she was 5-year-old. Physical examination revealed enlarged spleen. She was severely anemic, with a hemoglobin level of 4.6 g/dL. Initial laboratory tests showed a mild liver dysfunction. CT-Scan Abdomen revealed the absence of the portal vein with extensive collateral circulation, splenomegaly with infarction in the superior-lateral region, dilation and tortuosity of the splenic vein, superior mesenteric vein, and the post-splenic system veins (gastric vein, esophageal vein, and inferior mesenteric vein) which connected to the left renal vein.
Angiography found that the superior mesenteric vein and portosystemic vein were joined, forming collateral to the renal vein and subdiaphragmatic IVC, confirmed the diagnosis of CAPV type Ib. Echocardiogram before surgery revealed good ventricular function. No other abnormal congenital or genetic condition was detected. The patient underwent splenectomy and partial devascularisation surgery. Post surgery she was conscious and no evidence of GI bleeding.
Discussion: CAPV is a rare condition with clinical presentations that can range from no symptoms to systemic manifestations such as hepatopulmonary syndrome, encephalopathy, or hepatoma. Previous reports indicated the risk of HCC in CAVP patient. This case represent our experience in these rare and complex patient. Long term monitoring is necessary to identify any potential complications.
PP-01-290
Financial Toxicity of Colorectal Carcinoma in Sri Lanka: Preliminary Results
1Department Of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, National Hospital, Sri Lanka, Colombo
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Financial toxicity in cancer refers to the excessive financial strain on patients, families, and society due to cancer-related expenses. This study assesses the financial toxicity in a cohort of Sri Lankan patients with colorectal cancer.
Materials and Methods: Data from patients with colorectal carcinoma of any stage, diagnosed 6- 24 months prior, and receiving treatment from the National Cancer Institute were collected using an interviewer-administered questionnaire.
Results: Eighty-one patients were included. Financial toxicity was highly prevalent with 82.7%; (n=67) reporting a financial toxicity of 4 or 5 on a scale of 1 to 5. Transport (72, 88.9%), external investigations (55, 67.9%), supportive medications (53, 65.4%), and active cancer management (48, 59.3%) were the major factors contributing to financial burden. The main impacts of financial toxicity included compromised spending on food (40, 49.4%), children's education (23, 28.4%), and recreation (26, 32.1%). Financial toxicity resulted in 56 (69.1%) exhausting life savings, 35 (43.2%) losing property and 28 (34.6%) resorting to loans. Financial aid was received by 45 (55.6%), primarily from their relatives (36, 44.4%), with minimal support from the government (n=6, 7.4%) or the hospital (n=2, 2.5%).
Conclusion: Despite free healthcare in Sri Lanka, patients with colorectal carcinoma seeking treatment from the public health sector were found to be facing significant financial toxicity, often compromising basic day-to-day needs. Many had exhausted their life savings, lost property or had to resort to loans impacting the future economic stability of the whole household.
PP-01-291
Clinical characteristics and outcomes in hilar cholangiocarcinoma: A 6-year experience
1Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;2Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Sri Lanka;3University Surgical Unit, National Hospital Sri Lanka, Sri Lanka;4Department of Anesthesiology and Critical Care, University of Colombo
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Hilar cholangiocarcinomas (HC) are associated with poor outcomes. This study outlines the demography and outcomes of patients with HC from a single HPB surgical unit in Sri Lanka.
Materials and Methods: A retrospective analysis of the unit HC database from 2019 to 2024 was done.
Results: Forty-eight patients were included, with a mean age of 63.5 years; 29(60.4%) were male. Majority were ASA II (n=19,39.6%). The most common symptom was obstructive jaundice (n=37,77.1%), followed by anorexia and weight loss (n=30,62.5%). The mean duration of symptoms on presentation was 3 months. Most tumours were Bismuth type 4 (n=12,25.0%) and type 1(n=3, 6.3%), type 2 (n=9, 18.8%), type 3A (n=5, 10.4%), type 3B(n=6,12.5%). Staging laparoscopy was performed in 22 (45.8%) patients, with 11 revealing metastatic disease. 39 (77.1%) patients were inoperable, and only 9(18.8%) lesions were resectable. However, surgery was performed in only 6 (12.5%). There were two patients with poor performance status and one with cirrhosis which precluded curative resection. The majority (n=42,87.5%) received palliative care. Metastatic disease was found in 23(47.9%). Surgical procedures included extended right hepatectomy+extrahepatic biliary resection(EHBR) and hepaticojejunostomy (n=4) and left hepatectomy with EHBR+hepaticojejunostomy(n=2). Other interventions included ERCP+palliative biliary stenting (n=17,35.4%), external biliary drainage (n=15,31.3%), PTC (n=6,12.5%),EUS-BD (Hep-Gas) (n=1). Post-operative complications included pneumonia(n=3) and transient liver dysfunction(n=2). There was one postoperative mortality due to post-hepatectomy liver failure.
Conclusion: Locally advanced or metastatic disease and poor patient fitness have led to poor outcomes for hilar cholangiocarcinoma. Early referral to specialized surgical units should be emphasized.
PP-01-292
Subtotal cholecystectomy: a decade’s experience of a tertiary Hepatobilliary Surgical Unit
Githma Wimalasena1, Dulanja Senanayake1, S Sivaganesh2,3 and Duminda Subasinghe2,3
1Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;3University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: Laparoscopic cholecystectomy (LC) is the standard of care for symptomatic gallstone disease. Where visualisation of the cysto-hepatic triangle is compromised a subtotal cholecystectomy (SC) is a safe alternative to minimize bile duct injuries. This may be done laparoscopically or as an open procedure depending on the experience of the surgeon. This study describes the experience of a HPB surgical unit in SC.
Materials and Methods: A retrospective analysis of the unit LC database containing patient demography and operative notes from year 2015 to 2024 was done.
Results: Of the 284 LCs done during this period, 23 (8.1%) were SCs. The mean age of patients who underwent SC was 53.13 (SD – 16.75) years with Male : Female ratio 1 : 0.92. Of the SCs, 9 (39.13%) were performed laparoscopically and 14 were done as open procedures. Failure to demonstrate the cysto-hepatic triangle due to dense adhesions (n=20, 86.96%), an impacted stone in Hartmann’s pouch (n=2,8.69%) and presence of a cholecysto-duodenal fistula (n=1,4.35%) were indications for SC. The mean post-operative hospital stay was shorter for laparoscopic SC (1.44 days, SD-0.527) than open SC (4.38 days, SD-2.567) and was found to be statistically significant (p = 0.001). There was one postoperative mortality due to biliary sepsis. 12 (52.2%) patients who underwent SCs followed up over a mean period of 20.6 months were well without any recurrences.
Conclusion: SCs are a safe option for difficult gallbladders. Laparoscopic SCs have a significantly shorter postoperative hospital stay than open SCs.
PP-01-293
Ileocolic Intussusception in the Elderly: A Case Report
Fita Fitrianti1, Arlyando Saragih1 and Febiansyah Ibrahim2
1Department of Internal Medicine, Hermina Jatinegara, Jakarta Timur, Indonesia;2Digestive Division, Department of Surgery, Hermina Jatinegara Hospital, Jakarta Timur, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Intussusception is a condition in which a segment of the gastrointestinal tract invaginates into the lumen of another segment and recognised as a cause of bowel obstruction. Adult intussusception is less common than juvenile intussusception in terms of cause, appearance, and treatment.
Case Description: We report the case of a 71-year-old female patient who presented to the Emergency Department (ED) with upper abdominal pain, nausea, vomiting and weakness. The patient had ongoing mild intermittent abdominal pain for months. There were features of peritonism on clinical examination and this was associated with raised inflammatory markers. A Computed Tomography (CT) scan showed Ileocolic intussusception. The patient underwent emergency surgical resection. Meckel's diverticulum was found as a pathologic lead point in the resected specimen, with no evidence of malignancy.
Discussion: We presented a case of in an adult which is not commonly seen. Clinical history of chronic intermittent abdominal pain and CT abdomen are helpful in establishing the diagnosis. Despite that conservative approach is described in the literature, surgery continues to be the only option in patients who are unstable and show signs of peritonitis.
PP-01-294
The Masquerade of Hepatosplenic T-cell Lymphoma
Syuhada Dan Adnan
Hospital Sultanah Nur Zahirah, Kuala Terengganu, Kuala Terengganu, Malaysia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Hepatosplenic T-cell lymphoma (HSTCL) is an extremely rare and aggressive form of lymphoma. Unlike most common forms of lymphoma, swollen lymph nodes are uncommon in HCTL. Patients usually present with non specific constitutional symptoms and spleen and liver enlargement, with variable degree of cytopenia. The rarity of this disease, coupled with lack of lymph node involvement that usually seen in lymphomas, causes significantly difficulty in diagnosis and inevitably delays the initiation of treatment. The author reports the case of a young lady who admitted with jaundice and cytopenia. The diagnosis was challenging, initially mimicking autoimmune disease and it required an extensive investigation that finally revealed the ultimate diagnosis. This case highlights the difficulty in diagnosing HSTCL and the importance of considering diagnosis of hepatosplenomegaly in patients who present with constitutional symptoms and no significant lymphadenopathy.
PP-01-296
Relationship between simultaneous expression of ALDH and KRAS with endoscopic, histological characteristics of gastric cancer
Hoang Dong Duc1 and An Le Viet2
1Thai Nguyen University Of Medicine And Pharmacy, Thai Nguyen, Viet Nam;2Tien Du District Medical Center, Bac Ninh, Viet Nam
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To analyze the relationship between the simultaneous expression of ALDH and KRAS with endoscopic, histological characteristics in gastric cancer (GC).
Materials and Methods: Cross-sectional study on 103 patients with a confirmed diagnosis of gastric cancer and surgical resection of the tumor at K Hospital, Ha Noi, Viet Nam. Analysis of the relationship between the simultaneous expression of ALDH, KRAS, and endoscopic, histological features.
Results: The rate of GC in men and women was highest in the age group 60-69, accounting for 34.4% and 35.7%, respectively. Patients with GC in the antrum had the highest rate of simultaneous expression of both markers (56.0%), p > 0.05. Patients with ulcerative tumors expressing both markers had the highest rate (68.0%), p > 0.05. Patients with tubular adenoma had the highest rate of co-expression of both markers (66.0%), p > 0.05. Patients with low and moderately differentiated GC had the highest rate of simultaneous expression of both markers at 34.0%, with a difference in the simultaneous expression of both markers according to the degree of differentiation, p < 0, 05.
Conclusion: Simultaneous expression of ALDH and KRAS is associated with low and moderate differentiation according to histopathology. Simultaneous expression of ALDH and KRAS is not associated with endoscopic characteristics.
PP-01-301
Serum Interleukin 17 Level in Ulcerative Colitis and Crohn’s Disease: Inflammatory Bowel Disease Patients in Indonesia
Muhammad Faisal Prananda, Murdani Abdullah, Sukamto Koesnoe, Robert Sinto, Dadang Makmun and Ayu Suciah Khaerani
Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background: The incidence of IBD is increasing worldwide, with different characteristics in each country. IL-17 is a strong pro-inflammatory cytokine that plays a role in IBD. No studies have assessed serum interleukin 17 (IL-17) levels in IBD patients in Indonesia, which can reveal its role in the pathogenesis of IBD as well as proposed therapeutic modalities for IBD.
Aim: To determine the difference in serum IL-17 levels in patients with IBD.
Method: We conducted a cross-sectional study at Cipto Mangunkusumo National General Hospital from April 2022 to April 2023. The inclusion criteria were adult patients diagnosed with IBD and a healthy population who did not have any gastrointestinal complaints or a history of autoimmune diseases and malignancies. Data collection was carried out sequentially. Serum IL-17 levels were assessed using ELISA. Mann-Whitney tests and linear regression were carried out using the SPSS application.
Result: The total number of subjects in this study was 125, consisting of 93 IBD patients and 32 healthy subjects. Serum IL-17 levels were 4.13 (3.19-5.14), 4.30 (3.59-5.14), and 3.40 (2.97-4.01) pg/mL for the ulcerative colitis (UC), Crohn’s disease (CD), and healthy subjects (p=0.004). There was no difference of serum IL-17 levels between UC and CD. Multivariate analysis showed that disease activity (p=0.010) is associated with serum IL-17 levels in UC.
Conclusion: There was a significant difference in the median value of serum IL-17 levels between IBD and healthy population, but there was no differences between UC and CD.
PP-01-318
Pharmacological inhibition of HMGB1 ameliorates sepsis induced hepatic micro-vascular dysfunction and portal hypertension during cirrhosis
Vaibhav Tiwari, Rajni Yadav, Aishwarya Bhatnagar, S Himanshi, Savneet Kaur and Dinesh Mani Tripathi
Institute of Liver and Biliary Sciences, New Delhi, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the role of HMGB1 in sepsis induced hepatic microvascular-dysfunction and portal-hypertension in cirrhosis.
Materials and Methods: Experimentally, polymicrobial sepsis was induced by caecum ligation and puncture in non cirrhotic animals and by intraperitonial administration of LPS 1mg/kg in CCl4-cirrhotic animals.Controls(Ct1), Ct+CLP(Ct2), Ct+CLP+Glycyrrhizin(Ct3) were compared with CCl4 cirrhotic rats(Gr1), CCl4+LPS(Gr2), CCl4+LPS+Glycyrrhizin(Gr3). HMGB1 pharmacological inhibition was achieved by glycyrrhizin(GLZ). Hepatic hemodynamic was monitored followed by in-situ-exvivo Hepatic microvascular functionality analysis. Cellular, Molecular, Biochemical and histological analysis was performed in hepatic tissues.
Results: Studies shows a noticeable increased PP( 9.2±1; + 27.7% (CT2), vs 6.1±1 in CT1) a marked reduction in CT3 (8.1±.5; -15.2%) vs CT2(p=0.01) was observed.CT2 animals also had increased SMABF (p=0.001) and PBF (p= 0.05) in comparison to CT1 and CT3.However,in cirrhotic animals we observed significantly raised PP(12.6±1;+26%(Gr2), vs 10± 1; in Gr1) a marked reduction in Gr3(10.8 ±0.2;-14%) vs Gr2(p=0.01) was observed. Gr2 animals had increased SMABF (p=0.001) and PBF (p=0.001) in comparison to Gr1 and Gr3. Endothelial dysfunction assay under perfused ex-vivo condition showed improvement in endothelial activity showed by percentage change in Portal perfusion pressure (p=0.005) in Gr3&Ct3 vs Gr2&Ct2. Periportal inflammation and inflammatory cells infiltration was evidently reduced in both Ct3 and Gr3 rats vs Ct2&Gr2 respectively. Gene expression of TNF-alpha, IL-6, HMGB1,DPEP1, TLR4, RAGE were significantly upregulated in Gr2 and Ct2 (p<0.05) vs Gr3 and Ct3. Moreover, Endothelial dysfunction genes ICAM1,VCAM1,DPEP1 fold was down regulated (p<0.05) in Gr3&Ct3 vs Gr2&Ct2.Serum levels of ALT and AST and LDH were down regulated (p<0.05) in Gr3 & Ct3 vs Gr2 & Ct2.
Conclusion: Pharmacological inhibition of HMGB1 ameliorates PP and protects vascular function during sepsis in liver cirrhosis.
PP-01-335
Assessing Microplastic Impact on Gastrointestinal Health: Findings from Jakarta's Cross-Sectional Study
Liovicinie Andarini1, Nur Hamidah1, Ari Syam1, Pukovisa Prawiroharjo2,3, Anyelir Nielya Mutiara Putri2, Noryanto Ikhromi2 and Elizabeth Divina2
1Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;2Department of Neurology, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;3Universitas Indonesia Hospital, Jakarta, Indonesia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objectives: To measure microplastic exposure and its association with gastrointestinal symptoms using the Gastrointestinal Symptom Rating Scale (GSRS).
Materials and Methods: A cross-sectional study was conducted with 439 subjects from Jakarta and surrounding areas. Participants were selected using simple random sampling from those exposed to microplastics orally and met the inclusion criteria: willingness to participate, ability to complete the questionnaire, and age between 18-59 years. Data were collected using a validated questionnaire assessing microplastic exposure and gastrointestinal symptoms, with clinical assessment through the GSRS, categorized into five syndromes: diarrhea, indigestion, constipation, abdominal pain, and reflux. Plastic usage was categorized into three levels: low (less than once a month), moderate (once a month to 6 times a week), and high (once a day or more). Statistical analysis included descriptive statistics, normality test, and Kruskal-Wallis test, followed by post-hoc Mann-Whitney tests for significant differences.
Results: Analysis of 439 participants revealed a significant correlation between bottled water in plastic and GSRS scores for indigestion (p=0.025) and abdominal pain (p=0.030). No significant correlation was found between gallon water consumption and GSRS scores for any gastrointestinal syndrome (p>0.05). Plastic-packaged food consumption showed significant correlations with GSRS scores for diarrhea (p=0.002), indigestion (p=0.028), and constipation (p=0.034). Post-hoc analysis revealed significant differences between low vs. medium and low vs. high consumption for all significant symptoms across all groups (p<0.05).
Conclusion: The study demonstrates significant associations between microplastic exposure and gastrointestinal symptoms, suggesting that microplastics from plastic packaging may contribute to gastrointestinal issues.
PP-01-339
Zonula occludens 1 as a novel diagnostic biomarker in decompensated cirrhotic patients with hepatorenal syndrome
Balasubramaniyan Vairappan and Raj Kumar and Mukta Wyawahare
Jawaharlal Institute Of Postgraduate Medical Education And Research, Puducherry, India
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Objective: Hepatorenal syndrome (HRS) is a life-threatening complication of liver cirrhosis with renal failure. The common biomarkers to assess kidney injury have substantial limitations in cirrhotic patients. Here, we aimed to assess the circulating concentration of Zonula occludens (ZO) 1 and cystatin C in decompensated cirrhotic (DC) patients with HRS and to evaluate their diagnostic potential of HRS.
Design & Methods: DC patients with HRS and normal healthy volunteers (n=40, for both) were recruited in the study. Serum ZO-1, cystatin C and clinical chemistry parameters were analysed.
Results: When compared to control subjects, an increase in the concentration of ZO-1 (7.135 ± 0.283 vs 0. 788 ± 0.114; P < 0.0001) and cystatin C (2.92 ± 0.22 vs 1. 59 ± 0.04; P < 0.0001) were observed in DC patients with HRS. There was a positive correlation between serum ZO-1 and cystatin C (r=0.551, p<0.0001). Serum ZO-1 was also positively correlated with serum creatinine and MELD-Na (r=0.767, p<0.0001; r=0.841, p<0.0001, respectively).
Conclusion: Increased level of serum ZO-1 observed in DC patients with HRS was positively correlated with cystatin c and creatinine and has improved diagnostic capability than cystatin C. Thus, this study proves the potential of ZO-1 as a valuable biomarker in DC patients on the background of HRS. Indeed, this finding should be further confirmed with a large cohort of DC patients with HRS.
PP-01-341
Serum cryoglobulinemia in chronic hepatitis C patients after cure by anti-viral agents
1Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;2Faculty of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;3Department of Internal Medicine, Institute of Medical Sciences, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Background/Aims: Cryoglobulinemia is one of the major extra-hepatic manifestations of chronic hepatitis C infection. Persistent cryoglobulinemia after completion of antiviral treatment is an important consideration of clinical management in chronic hepatitis C patients. We aimed to investigate the occurrence of serum cryoglobulinemia in chronic hepatitis C patients without cryoglobulinemia at the initiation of antiviral treatment after cure.
Methods: Totally 776 patients without cryoglobulinemia were assessed for serum cryoglobulinemia after completion of anti-HCV treatment. Serum cryoglobulinemia precipitation was assessed both initiation and completion of the treatment and analyzed for the clinical, and laboratory factors associated with chronic hepatitis C.
Results: There were 118 patients were checked for serum cryo-precipitation after completion of the treatment and 8 patients (4.6%) were positive for serum cryoglobulinemia. Patients who became positive cryoglobulinemia had a higher proportion of liver cirrhosis patients (4/50%, p=0.033) and other organ cancer patients (5/62.5%, p=0.006) than patients who remained no sign of cryoglobulinemia after treatment. In multivariate analysis, liver cirrhosis (Odds Ratio [OR]–17.86, 95% Confidence Interval [95% CI]– 1.79-177.35, p = 0.014) and other organ cancer (OR-25.17 95% CI– 2.59-244.23, p = 0.005) were independently and significantly associated with positive cryoglobulinemia 3 months after antiviral treatment.
Conclusions: A 6.7% prevalence of cryoglobulinemia three months after the antiviral DAA therapy was observed. Liver cirrhosis and other organ cancer were independently and significantly associated with positive cryoglobulinemia after antiviral treatment. Further investigation into the causes of positive cryoglobulinemia after DAA antiviral therapy is necessary.
PP-01-344
Study of overall survival and factors affecting outcomes in chronic HCV patients undergoing liver transplantation
Kongpob Yongrattanakit
Khonkaen University, Khonkaen, Thailand
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
-Primary outcome: overall survival of LT recipients with chronic HCV infection
-Secondary outcomes: Factors affecting overall survival, rate of HCV, cirrhosis, and HCC recurrence.
-16-year-period, medical records of adult LT recipients with HCV infection, transplanted between January 2008 and May 2023, were reviewed. Recipients with only anti-HCV positivity and lacking confirmation of HCV VL before transplantation were excluded. Patient medical records were abstracted to gather information. Survival curves were generated using Kaplan-Meier method and compared using log-rank test. Univariate Cox proportional hazard models were employed to calculate hazard ratios for variables associated with death.
-The medical records of 81 adult liver transplantation recipient with chronic HCV transplanted at Srinagarind Hospital. HCV recurrence was observed in 98.6% of all cases. Cirrhosis recurrence occurred in 4.9%, HCC recurrence was noted in 7.4%. 5-year overall survival rate for LT recipients was 75.3%. For factors affecting outcomes, HCC recurrence (HR, 26.55; 95% CI; P < 0.001) and cirrhosis recurrence (HR, 9.05; P = 0.008) were found to be correlated with increased risk of mortality. Both HCV treatment after LT (HR, 0.09; P < 0.001) and achieving SVR at final follow-up visit (HR, 0.13; P < 0.001) were significantly associated with reduced risk of death.
-Over 16-year peroid, 5-year overall survival rate for LT recipients with HCV infection was 75.3%. Recurrence of HCC and cirrhosis were significant risk factors for reducing overall survival. Conversely, LT patients who underwent HCV treatment and achieved SVR status at final follow-up visit demonstrated good factor to improve overall survival.
PP-01-353
An adrenal pseudocyst masquerading as a pancreatic neoplasm – A case report
Padmakumara Udapamunuwa, Melan Jayasinghe, Nayana Ratnayake and Mohammed Mikram and Janusha Udapamunuwa
National Hospital Kandy, Kandy, Sri Lanka, 2Department of Medicine, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka
Poster (Day 1), Poster Area, November 22, 2024, 8:30 AM - 5:00 PM
Introduction: Adrenal pseudocysts are seldom encountered, non-functional cysts of the adrenal gland, located retroperitoneally. Accurate preoperative diagnosis is challenging due to their resemblance of other cystic lesions, especially those of pancreatic origin. This report discusses the diagnostic and surgical approach to a large adrenal pseudocyst initially misdiagnosed as a pancreatic neoplasm.
Case description: A 36-year-old, ASA-1 female presented with vague back pain and non-specific abdominal discomfort. Ultrasound scan revealed a large unilocular cyst over the body and tail of the pancreas. Rest of the investigations were normal. A contrast enhanced computed tomography scan identified a 13x11x10 cm cyst with no communication to the pancreatic ducts. Initial diagnostic impressions included pancreatic pseudocyst or mucinous pancreatic neoplasm. Following the multidisciplinary team discussion, the patient was prepared for a distal pancreatectomy with possible splenectomy. Intraoperatively, a large retroperitoneal cystic mass was found. It was separate from the pancreas and located on the superior pole of the left kidney. The mass was resected en bloc. Histology of the specimen revealed an adrenal pseudocyst.
Discussion: Adrenal pseudocysts are benign cortical cysts devoid of an epithelial lining. They are rare, with only about 250 cases being reported previously. Due to their proximity to the other retroperitoneal organs, radiological delineation of the lesion is difficult. This case highlights the importance of considering an adrenal pseudocyst as an important differential diagnosis for retroperitoneal cystic lesions. If diagnosed correctly, simple cystectomy can be planned and this will avoid unnecessary preoperative preparations.
Graph/Image/Table
PP-02-001
Multiple colonic diverticular bleeding: a case report
Josephine Amanda Setiadi, Bradley Jimmy Waleleng, Fandy Gosal, Luciana Rotty, Jeanne Winarta and Andrew Waleleng
Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia, Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Colonic diverticular bleeding is the primary cause of acute lower gastrointestinal bleeding as a complication of diverticulosis.1 Less than 5% of patients with diverticulosis presents with bleeding. In Asian countries, diverticula are predominant in the right-sided colon, in contrast to the sigmoid colon in Western countries.2
Case Illustration: A 63-year-old woman was referred to our gastroenterology division with complaints of moderate volume hematochezia since two days before admission. The patient denied abdominal pain. She reported a previous history of hematochezia five years ago which resolves spontaneously and hypertension treated with amlodipine 5mg daily since five years ago. Her vital signs were normal. On physical examination, the patient appeared anaemic and abdominal examination was unremarkable. Digital rectal examination revealed internal haemorrhoid and bloody stool. Blood tests revealed hemoglobin of 10.4g/dL. Colonoscopy revealed active bleeding from multiple ascending colon diverticulum, proctosigmoiditis, and internal hemorrhoid. The bleeding was controlled using haemostatic clips. The patient condition improved and discharged without complications.
Discussion: We documented a case of diverticular bleeding in a 63-year-old female patient. The likelihood of diverticular bleeding increases with advancing age.2 Diverticular bleeding usually stops spontaneously but in this case, continued more than two days. A previous history of hematochezia and hypertension might contribute to rebleeding.3 Right-sided diverticular disease is associated with higher incidence of bleeding complications and colonoscopy revealed multiple ascending colon diverticular bleeding.1 Haemostasis was achieved using haemostatic clips which is known to be effective and had a low risk of rebleeding.4 The patient recovered well after 1 week follow-up.
PP-02-002
Endoscopic therapy with high concentration dextrose: a comeback with a new twist
Shahreedhan Shahrani1, Bo Shen2 and Ida Normiha Hilmi1
1University Malaya Medical Centre, Kuala Lumpur, Malaysia;2Center for Inflammatory Bowel Diseases, University Irving Medical Center/ NewYork-Presbyterian/Columbia, New York, USA
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Diffuse non-variceal, non-arterial bleeding, albeit common, can be quite an endoscopic challenge. Standard methods of endoscopic therapy, may not be effective due to the inability to pinpoint an exact location of bleeding. Intra-lesional high concentration dextrose has been used in managing variceal bleeding historically. There have been limited reports of its use in managing other causes of diffuse bleeding.
Case description: We report a case of a 61-year-old gentleman, who presented with a history of altered bowel habit and an acute history of per rectal bleeding. He was hemodynamically stable; however, hemoglobin showed a reduction of 3g/L. A colonoscopy was performed, revealing a fungating, circumferential, friable and bleeding mass 10cm from anal verge extending for approximately 10cm. There was diffuse, active oozing of blood from the lesion, even prior to multiple biopsies being taken for histopathological examination (HPE). A decision was made to use high concentration dextrose to spray on the diffuse mucosal bleeding site, after specimen biopsies were taken. A through-the-scope spray catheter (Olympus, tubing length 1650mm) was used to spray 12ml of dextrose 50%. Complete hemostasis was achieved. There were no more further drop in hemoglobin levels or further episodes of acute PR bleeding until his semi-elective surgery for tumor resection was arranged.
Discussion: The consideration behind using hypertonic glucose for diffuse mucosal GI bleeding is similar to that of its’ use in gastric varices, through osmotic dehydration. Endoscopic therapy with high concentration dextrose may be considered as an alternative method for managing diffuse non-variceal, non-arterial bleeding.
PP-02-003
A case of esophagectomy and subtotal-stomach reconstruction for esophago-mediastinal fistula after omentoplasty for graft infection
Rai Shimoyama1, Takaaki Murata1, Yuma Suno1, Naoko Isogai2, Jun Kawachi2, Daisuke Hama1, Yusuke Gunji1, Motoki Nagatsuka1, Takeshi Yamabe1 and Kenichiro Noguchi1
1Shonan Kamakura General Hospital, Kamakura, Japan;2Hayama Heart Center, Hayama, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: We report a case of a patient who underwent subtotal stomach reconstruction with esophagectomy for esophago-mediastinal fistula after omentoplasty for graft infection after graft replacement for thoracic aortic dissection.
Case Report: The patient was a 48-year-old woman after aortic arch replacement and open stent implantation for thoracic aortic dissection. The patient underwent omentoplasty 1 year 4 month after the initial surgery and re-total arch replacement 2 year 1 month after the initial surgery for graft infection. Patient was diagnosed as esophago-mediastinal fistula 1 month after the last operation, but fistula closure with Over-The-Scope-Clip or esophageal stent were all unsuccessful and the infection was uncontrolled. Subtotal esophagectomy was performed 3 years 7 months after the initial operation. As the right gastroepiploic artery was devided at the time of the omentoplasty, subtotal stomach reconstruction was performed to preserve the blood flow on the lesser curvature. The postoperative course was good, without recurrence of fistula or graft infection.
Discussion: In this case, conservative treatment was unsuccessful and esophagectomy had to be chosen. However, because the right gastroepiploic artery was dissected at the time of surgery for omentoplasty, there was a high risk of gastric tube necrosis with conventional gastric tube reconstruction. Colonic reconstruction was considered, but due to the complexity of the surgical technique, reconstruction by subtotal stomach reconstruction was chosen.
In cases where blood flow on the greater curvature side is expected to be insufficient, subtotal gastric reconstruction with preservation of the lesser curvature may be an effective option.
PP-02-004
Emphysematous Gastritis with Polymicrobial Etiology in a Gouty Arthritis Patient
Jose Luis Matthias Sollano and Gerardo Pedregosa
Makati Medical Center, Makati, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Emphysematous gastritis (EG) is a rare condition characterized by gas accumulation within the gastric wall. This case report presents a patient with EG from a polymicrobial infection with an acute kidney injury complicated by gouty arthritis.
Case Description: A 53-year-old male with gouty arthritis presented with severe abdominal pain, chills, diarrhea, and vomiting. Ancillaries showed leukocytosis at 12x10^3/uL and creatinine 2.18mg/dL. Imaging revealed air locules at the posterior wall of the gastric fundus and tubular air-filled foci in certain hepatic segments and vascular structures. The patient was given antibiotics, hydration and placed on NPO with an NGT. Endoscopy identified an infected gastric mass and ulcer, from which Neisseria flava/perflava/subflava, Serratia marcescens, and Streptococcus salivarius were cultured with resistance. Broad-spectrum antibiotics, total parenteral nutrition and supportive care was given. During the admission, the gouty arthritis was in flare at the left knee and right foot which was treated with IV corticosteroids. The patient had a repeat of the imaging and endoscopy, both showing significant improvement in the initial findings. He was subsequently discharged stable and completed antibiotics for 14 days.
Discussion: With high mortality rates of about 60-80%, the importance of comprehensive diagnostic workup and appropriate management is crucial to the survival of patients with EG. This case, complicated by a polymicrobial nature and ongoing flare of gouty arthritis, emphasizes early recognition and management are crucial for optimal patient outcomes. Further studies are needed to elucidate the pathophysiology of this rare condition and to identify potential risk factors.
PP-02-005
A Puzzling Case of Recurrent Fever, Jaundice, and Tea-Colored Urine in a 32-Year-Old Male
Jose Luis Matthias Sollano
Makati Medical Center, Makati, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: This case report details the diagnostic journey of a 32-year-old male with a perplexing three-year history of recurrent fever, jaundice, tea-colored urine, and occasional acholic stools, each episode spontaneously resolving.
Case Description: Exhaustive investigations initially yielded inconclusive results, challenging the conventional diagnostic paradigm. Subsequent evaluation, including liver function tests and imaging studies, revealed subtle abnormalities, prompting a reevaluation of potential infectious etiologies. The breakthrough in diagnosis came with a positive result in the QuantiFERON®-TB Gold test, unveiling the presence of Tuberculosis (TB) as an underlying cause for the recurrent symptomatic episodes. Additional confirmation came along with the resolution of the symptoms when Anti-Koch’s treatment was initiated.
Discussion: This report discusses the challenges faced in diagnosing Hepatic Tuberculosis, emphasizing the need for heightened clinical suspicion and a comprehensive diagnostic approach. It also underscores the importance of timely identification and initiation of appropriate anti-tubercular therapy to prevent further complications and improve patient outcomes.
This case serves as a poignant reminder of the diverse clinical presentations of tuberculosis, urging clinicians to maintain a broad differential diagnosis and consider rare manifestations in the pursuit of resolving diagnostic conundrums associated with recurrent fever, jaundice, and tea-colored urine.
PP-02-006
Olmesartan induced enteropathy masquerading as celiac disease
Pulkit Sondhi, Neha Berry, Amrish Sahney, Manav Wadhawan, Rameez Raja Najar and Ajay Kumar
Blk Max SuperspecialityHospital New Delhi, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Backround: Olmesartan, an antihypertensive medication has been rarely associated with sprue like enteropathy clinically presenting with chronic diarrhea, weight loss and villous atrophy on biopsy.
Case Description: We describe a 67 year old female known case of hypertension on Olmesartan for many years presented with recurrent episodes of intermittent diarrhea complicated with dyselectrolytemia and renal failure. Though patient had endoscopic evidence suggestive of celiac disease (CeD) with equivocal biochemical tests and Feature of Ulcerative colitis (UC) on colonoscopy. Patient was on strict Gluten free diet (GFD) for one and half year with no improvement and also received oral Steroids and immunomodulatory therapy/ small molecules for possible steroid dependent UC. Despite receiving treatment for both CeD and UC, patient continued to have intermittent intractable symptoms. After stopping olmesartan considering as possible culprit agent, patient showed drastic improvement in symptoms thus reinforcing the diagnosis of Olmesartan induced Enteropathy (OIE).
Discussion: Clinicians and patients should be aware that olmesartan can cause an enteropathy clinically and histopathologically similar to celiac disease as well as a colopathy similar to microscopic colitis. Failure to recognize olmesartan-induced enteropathy may result in patients continuing on a medication that is injurious to the gastrointestinal tract or embarking on an unnecessary and expensive medical evaluation, frequently with steroid use, with both options disrupting quality of life. Time to recognition of this entity is often prolonged, suggesting the need for increased awareness in both patients and clinicians.
PP-02-007
Splenic Abscess: An Unexpected Clue to Underlying Malignancy
Christine Marianne Sy, Rial Juben De Leon and Rona Marie Lawenko
De La Salle University Medical Center, Dasmariñas City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Splenic abscess is a relatively uncommon medical condition, with reported incidence rates ranging from 0.05% to 0.7%. This type of abscess typically occurs in individuals who are immunocompromised. This is a case of a 71 year old female presenting with splenic abscess masking a malignancy.
Case Description: A 71 year old Filipino female with uncontrolled diabetes presented with fever and left upper quadrant pain. Workup showed leukocytosis with neutrophilic predominance and imaging showed splenic abscess. Broad-spectrum antibiotics were administered, and percutaneous drainage was done. Culture of abscess showed Escherichia coli. Post drainage, there were persistent fever and left upper quadrant pain. Repeat CT scan then showed a tumor measuring 10.9 x 7.5 x 10.9 cm in the inferior aspect of the spleen with mass effect. A more definitive management is offered based on the biopsy results.
Discussion: Splenic abscess is more often secondary to hematogenous bacterial seeding. Only a few reported cases were documented to be from a tumoral necrosis. Splenic abscess may be clinically diagnosed based on the triad of fever, left upper quadrant pain, and leukocytosis. However, imaging is still essential. Treatment guidelines on splenic abscess are limited due to its rarity. Empiric broad-spectrum antibiotics are initiated once splenic abscess is suspected. Splenic abscess alone may be managed conservatively with percutaneous drainage and aggressively with splenectomy, with no difference in mortality based on studies. However, in the background of malignancy, treatment options may vary.
PP-02-008
A case of fractured and migrated Fully Covered SEMS removed by peroral cholangioscopy.
Shinya Tajima, Ken Ito, Michihiro Saitou, Masataka Kurihara, Yuto Yamada, Yousuke Okamoto and Manabu Watanabe
Toho University Ohashi Medical Center, Tokyo, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Fully Covered Self-Expanding Metal Stents (FC-SEMS) are recommended as a treatment option for benign bile duct stricture associated with chronic pancreatitis.
Complications such as fracture and migration of FC-SEMS are rare, and there is no standardized treatment for these issues.
Case Description: A 50-year-old man with chronic pancreatitis and benign bile duct stricture periodically underwent plastic stent (PS) replacement. Due to a lack of improvement, FC-SEMS (M-Intraductal; Medico's Hirata Inc., Osaka, Japan) was placed and scheduled for stent removal after four months. However, 3.5 months after stent placement, he developed acute cholangitis. Abdominal CT revealed a fractured FC-SEMS and dilated intrahepatic bile ducts. Urgent ERCP revealed that the FC-SEMS had migrated into the proximal bile duct. The FC-SEMS retrieval was unsuccessful, so we temporarily placed a PS, and scheduled the retrieval for the next session using peroral cholangioscopy (POCS). The fractured FCSEMS was kept in a clear view using POCS and successfully removed using grasping forceps (Spy Bite MAX, Boston Scientific).
Discussion: POCS was effective for troubleshooting the retrieval of FC-SEMS. These techniques sometimes result in a successful outcome for rare fractured and migrated cases.
PP-02-009
A Little Old Lady's Hernia Causing Small Bowel Obstruction
Erika Johanna Tañada-Escanlar
UST Hospital, manila, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Obturator hernia nicknamed as little old lady's hernia is an extremely uncommon pelvic and intra-abdominal hernia with an incidence of 0.07-1.4% affecting mostly emaciated elderly woman. It has the highest morbidity and mortality rates of all abdominal wall hernias and a significant cause of intestinal obstruction thus early recognition is paramount. We report a case of a gaunt 75-year-old woman who presented with small bowel obstruction due to incarcerated obturator hernia, successfully managed surgically.
Case Description: A gaunt 75-year-old woman presented with 7 days periumbilical abdominal pain along with bilious vomiting and abdominal distension. Clinical examination revealed abdominal distension, hyperactive bowel sounds and absence of tenderness. She has a surgical scar for two cesarean sections. Biochemical parameters were normal. Plain radiograph of abdomen revealed dilated small bowels with multiple air fluid levels. A computed tomography (CT) scan revealed herniation of the distal small intestinal loops into the right inguinal region through the obturator foramen.The patient underwent an exploratory laparotomy and a mesh-plug hernioplasty. During follow-up, there was no evidence of recurrence or complications.
Discussion: Obturator hernia refers to the herniation of intra-abdominal contents through the obturator canal causing 0.2% to 1.6% of all small bowel obstructions. In an emaciated elderly female presenting with intestinal obstruction, obturator hernia is an important differential diagnosis. Early recognition is essential in order to provide optimum surgical treatment to significantly reduce the mortality and morbidity rates.
1Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia;2Department of Internal Medicine, Atma Jaya Academic Hospital, North Jakarta, Indonesia;3School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Chronic intestinal pseudo-obstruction (CIPO) is a rare and perplexing gastrointestinal motility disorder characterized by symptoms mimicking mechanical bowel obstruction without detectable blockage. Typically, CIPO leads to significant morbidity, including malnutrition and a diminished quality of life. Spontaneous resolution of CIPO is exceedingly rare, making it of significant clinical interest.
Case Description: We present a compelling case of a 30-year-old female with a seven-year history of CIPO, characterized by recurrent spontaneous episodes of abdominal distension and pain, resolving within minutes to hours. Remarkably, despite the chronicity, the patient maintained stable nutritional status and carried out her daily activities without significant disruption. Extensive diagnostic evaluations, including ultrasonography, barium follow-through, gastroscopy, colonoscopy, and abdominal computed tomography scans, revealed no structural abnormalities. However, an incidental Helicobacter pylori infection was found and treated without symptomatic improvement. Notably, symptom frequency and severity correlated with her menstrual cycle and began subsiding two years after completing medical education, eventually leading to a complete resolution.
PP-02-011
Lafora-like Inclusion Bodies are associated with Polypharmacy
Leia Teo
MOHH, Changi General Hospital, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The differentials for the presence of lafora like inclusion bodies are increasingly diverse - ranging from fatal neurological afflictions such as Lafora disease to more common liver-related conditions
like hepatitis B infection. With growing access to healthcare, polypharmacy is also a crucial differential to consider.
Case Description: Mr CYS is a 69yo Chinese male with polypharmacy whose liver biopsy revealed lafora like inclusion bodies. A thorough review of his extensive medical history, medications and histological slides was performed. The differentials for his histological findings of interest were systemically ruled out through further investigations and clinical assessment by relevant specialists.
Discussion: This paper supports the growing evidence that Lafora like inclusion bodies are associated with polypharmacy - which includes chemotherapy, supplements and common medications like painkillers. Recognising this avoids the mislabelling of serious conditions and misguided treatment. Additionally, as such inclusion bodies may be detrimental to cells, reduction of polypharmacy should be considered.
PP-02-012
Addressing the Treatment Gaps for Severe Crohn's Disease in SEA: A Case of Infliximab-Associated Sepsis
1Faculty of Medicine, Chulabhorn International College of Medicine (CICM), Thammasat University, Pathumthani, Thailand;2Department of Medicine, Gastroenterology Unit, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand;3Department of Medicine, Chulabhorn International College of Medicine (CICM) at Thammasat University, Pathumthani, Thailand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: This paper highlights the challenges and limitations in treating severe Crohn's disease in Thailand, particularly focusing on the use of biologic therapy in the presence of occult HBV infection.
Case Description: A case report of a 67-year-old Thai male with a complex medical history. The patient was diagnosed with stricture colonic Crohn's disease refractory to budesonide and treated with infliximab and azathioprine, leading to severe complications including drug-induced bone marrow suppression, PCP pneumonia, occult HBV infection, and severe sepsis. Despite aggressive treatment, the disease progressed. In Thailand, only a few biological treatments are available and are extremely limited under universal coverage, presenting significant risks to this patient.
Discussion: This case underscores the rarity and complexity of severe Crohn's disease in Southeast Asia, where the prevalence is low and treatment guidelines are limited. The use of infliximab necessitated antiviral prophylaxis due to the increased risk of HBV reactivation. Detailed endoscopic findings showed progressive disease with significant clinical challenges. The need for region-specific treatment protocols and the importance of screening for latent infections before initiating biologic therapy are emphasized. Comparisons with existing literature and guidelines highlight gaps and propose potential improvements. This case report illustrates the critical need for tailored IBD management guidelines in Thailand and similar regions. Awareness of the risks associated with biologic therapies, particularly in patients with latent infections, is essential. Proper preparation and individualized treatment plans are necessary to improve outcomes and reduce severe complications.
PP-02-013
Young Woman with Autoimmune Hepatitis: A Rare Case
Lioni Tjoeng and Muhammad Yamin Lubis
Department of Internal Medicine, EMC Sentul Hospital, Bogor, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Autoimmune hepatitis (AIH) is a heterogeneous immune-mediates liver disease that, in most cases, has effective treatment when diagnosed promptly and treated judiciously. Clinically, patients with AIH are characterized by raised serum ALT activity, hypergammaglobulinemia, non-organ-specific autoantibodies, & a chronic relapsing hepatitis, associated with a plasma cell hepatic infiltrate.
Case Description: A 22-year-old asian woman came with a complaint of maculopapular rash all over her body since 1 month ago. The rash can be itchy and uncomfortable. Other complaints include weakness, hair loss and weight loss. She also never consumed alcohol and took medication. Laboratory values revealed elevated AST(407 U/L), ALT(415 U/L), Gamma GT(178 U/L), ALP(227 U/L), & Globulin(4 g/dL). The SMA was positive, and ANA IF was speckled pattern with titre 1:100. Meanwhile, the results of AMA, anti LKM-1,SLA/LP,LC-1, ANA profile, hbsag, anti-hcv, & anti-hav IgM were all negative. The result of MRCP was splenomegaly. Liver biopsy could not be performed due to patient refusal. Treatment with prednisone 60 mg/day was initiated and the drug dose was reduced slowly according to the patient's clinical condition. Her condition began to improve, skin lessions on her skin disappeared and her lab test results returned to normal.
Discussion: In determining the diagnosis and treatment of patients, we use the IAIHG scoring system with a result of 13 (possible diagnosis of AIH) because there are examinations such as liver biopsy that cannot be carried out. The aim of this case report is to increase awareness of the complications of AIH and prevent irreversible morbidity.
Walsall Healthcare Nhs Trust, Walsall, United Kingdom
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
55-year-old male presented with worsening abdominal pain, distension, and low-grade fever over the past few weeks. He had a history of decompensated cirrhosis secondary to alcohol. The patient denied any recent travel, intravenous drug use, or recent hospitalizations.
On examination, the patient appeared ill with low-grade fever (38.0°C) and was hemodynamically stable. His abdomen was distended, with localized tenderness in the lower quadrants. Fluid wave was absent, but ultrasound revealed loculated ascitic pockets. There was no evidence of hepatic encephalopathy or jaundice.
Initial laboratory tests showed mildly elevated white blood cell count, with a predominantly neutrophilic response. Liver function tests showed elevated transaminases and hypoalbuminemia. Ascitic fluid analysis revealed an elevated total protein concentration and low serum-ascites albumin gradient (SAAG), suggestive of secondary peritonitis rather than uncomplicated cirrhotic ascites. A computed tomography (CT) scan showed no evidence of perforated viscus.
Ascitic fluid cultures were positive for *Rothia mucilaginosa*, an unusual finding typically associated with immunocompromised states. Blood cultures were negative.
The patient was diagnosed with loculated ascites secondary to cirrhosis, complicated by infection with *Rothia mucilaginosa*. Broad-spectrum antibiotics, including vancomycin, were initiated while awaiting sensitivity results, and a percutaneous catheter was placed for drainage of the loculated fluid collections. He was given albumin infusions with careful fluid management.
This case highlights the importance of considering atypical organisms in infected ascitic fluid, particularly in patients with cirrhosis and complex ascitic presentations like loculated ascites. Early recognition and targeted treatment are crucial for managing these infections and preventing further complications.
PP-02-015
The Role Of Manometry in Refractory GERD
Amanda Pitarini Utari, Jessica Wijaya, Liovicinie Andarini and Ari Fahrial Syam
FKUI, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: High-resolution manometry (HRM) is crucial for patients suspected of gastroesophageal reflux disease to rule out conditions with similar findings, such as achalasia and rumination syndrome. Despite its importance, manometry availability is limited in many parts of Indonesia. This study highlights the vital role of manometry in diagnosing and managing persistent GERD.
Case Description: A 37-year-old man presented with a two-month history of heartburn and difficulty swallowing, along with nausea when eating solid food, and feeling sensations of food being lodged in his esophagus. He had a history of GERD diagnosed three years prior and hematochezia due to an anal lump. Despite weight loss, his physical examination was unremarkable. Esophagogastroduodenoscopy (EGD) revealed grade A esophagitis and moderate gastritis with a differential diagnosis of achalasia, and biopsies showed no significant findings. A pH impedance assessment led to a diagnosis of refractory GERD, with a DeMeester Score of 28.32. Initial manometry indicated ineffective esophageal motility. The patient was treated with Rabeprazole, Domperidone, Rebamipide, and Ondansetron. Follow-up manometry results were normal.
Discussion: EGD has low diagnostic accuracy for GERD, as only 30% of patients have erosive reflux disease. Manometry plays a crucial role in excluding other diagnoses in GERD patients with normal mucosa or nonspecific esophagitis findings. Patients with GERD may be found with Esophageal-Gastro Junction (EGJ) incompetence as well as ineffective esophageal motility (IEM). Severe IEM serves as a key indicator of a more serious form of GERD.
PP-02-016
Upadacitinib induction and maintenance therapy in severe Crohn’s disease: A case report
Nicholas Wan1,2, Edward Young1,2, Arvind Rajagopalan1,2, Barbra Andrin1 and Dharshan Sathananthan1,2
1Department of Gastroenterology, Lyell Mcewin Hospital, Elizabeth Vale, Australia;2Adelaide Medical School, University of Adelaide, Adelaide, Australia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Severe Crohn’s colitis can be difficult to treat medically and can be associated with significant morbidity. We present a case of Upadacitinib to treat severe Crohn’s colitis.
Case Description: A 34-year-old male with a past medical history of ulcerative colitis presented with acute severe colitis. He was passing 9 bloody stools daily with a CRP of 68, Hb of 104, HR of 109 and febrile to 38.4°C. Gastrointestinal ultrasound (GIUS) demonstrated active inflammation of the sigmoid, descending and transverse colon characterised by bowel wall thickening, mesenteric hypertrophy and increased doppler signal (Image D, E, F). Flexible sigmoidoscopy revealed sparing of the rectum (Image A) with severe inflammation of the sigmoid colon (Image B, C) characterised by deep ulceration and spontaneous bleeding. The diagnosis was revised to severe Crohn’s disease and the patient was commenced on IV hydrocortisone and Infliximab. He failed to respond clinically and on day 3 of admission chose to proceed with commencement of Upadacitinib to avoid surgery. He was commenced on Upadacitinib 45mg daily and demonstrated a rapid clinical and biochemical response within 3 days (2 stools daily with no blood and normalisation of CRP). 8 weeks post admission, he continued to remain symptom free and repeat GIUS confirmed transmural healing (Image, G, H, I).
Discussion: This case report contributes to the growing evidence of Upadacitinib as a rapidly acting salvage treatment for patients with severe colitis who have failed rescue therapy with Infliximab.
PP-02-017
FUAS combined with ICIs in the treatment of solid tumor with liver metastasis:2 case reports
Ting Wang
Chongqing University Cancer Hospital, Chongqing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: FUAS is a local non-invasive therapy that precisely focuses the ultrasound beam on a specific target, so that high energy can be concentrated on a very small focus inside human body, where the tissue can be ablated. At present, it has been used to treat unresectable primary liver tumors and liver metastases, and it is well tolerated even in patients with poor Child-Pugh score. Compared with TACE and other methods, the treatment of liver metastases can further block the portal vein blood supply of liver metastases, and may have a more positive impact on the immune microenvironment than radiotherapy.
Case Descripition: This case report describes two patients with liver metastases from advanced solid tumors, who both used FUAS as a local treatment for liver metastases or primary lesions. One case was treated with gemcitabine based systemic chemotherapy combined with PD-1 inhibitor; another patient could not tolerate the side effects of chemotherapy, so he chose anti angiogenic drugs combined with PD-1 inhibitors for treatment. FUAS was performed at the beginning of treatment, when the disease progression was identified or systemic drug resistance occurred. The results showed that the tumor growth was effectively suppressed.
Discussion: During the treatment of these two patients, it was found that FUAS could be used as local treatment measure combining PD-1 inhibitor. The treatment itself did not show obvious side effects, nor did it increase the side effects of other systemic drugs.
PP-02-018
Malignant Gastric Tumor After Helicobacter Pylori Infection: A Case Series
Franzeska Anna Dewi Mursita Widinartasari1, Cecilia Oktaria Permatadewi1, Agung Prasetyo1, Didik Indiarso1, Hesti Triwahyu Hutami1, Erik Prabowo2, Abdul Mughni2, Ahmad Fathi Fuadi2, Udadi Sadhana3, Ika Pawitra Miranti3, Faiza Rizky Aryani Septarina3, Dik Puspasari3, Meira Dewi Kusuma Astuti3, Hirlan Hirlan1 and Hery Djagat Purnomo1
1Departments of Internal Medicine, Division of Gastroenterohepatology, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;2Departments of Digestive Surgery, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;3Departments of Anatomic Pathology, Faculty of Medicine, Diponegoro University, Dr. kariadi Hospital, Semarang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Helicobacter pylori has increasingly drawn attention for its potential link to the development of malignant tumors. In Indonesia, where H. pylori infection rates are low (10.4-11.5%) and gastric cancer incidence is correspondingly low (ASR 1.22/100,000), we present two cases of H. pylori-positive patients who developed gastric cancer.
Case Description:
Case 1: A 35-year-old woman presented with severe vomiting due to gastric outlet obstruction from pyloric stricture linked to H. pylori infection. After unsuccessful medical management, she underwent balloon dilatation followed by Roux-en-Y Gastrojejunostomy. Six months post-surgery, she developed acute cholangitis, requiring laparotomy, which uncovered a pyloric antrum mass with reactive lymphoid hyperplasia. Subsequently, she returned with ileus, prompting another laparotomy that revealed severe adhesions. Omentum biopsy revealed a malignant round cell tumor, possibly poorly differentiated adenocarcinoma or non-Hodgkin's lymphoma.
Case 2: A 62-year-old man presented with persistent vomitus. He had history of distal gastrectomy for gastric adenocarcinoma with H. pylori infection diagnosed one year prior. Post-surgery abdominal CT scan initially showed no residual mass. Current examination revealed evidence of gastric outlet obstruction due to recurrent mass formation. Endoscopic examination confirmed a mass obstructing the pylorus, with H. pylori infection. Due to the tumor's unresectable nature, the patient underwent triple bypass surgery. Omentum biopsy revealed metastatic adenocarcinoma likely originating from the stomach.
Discussion: This case highlights the potential rise of H. pylori cases in regions with low prevalence. It emphasizes the necessity for additional research about risk factors associated with malignancy following H. pylori infection, especially in low prevalence regions.
PP-02-019
42 year old male clinically diagnosed with cowden syndrome: a case report
Pamela Bianca Yap, Mara Mangio-Manansala and Frinz Moey Caunes Rubio
St.lukes medical center, Quezon city, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Cowden syndrome is part of PTEN hamartoma tumor syndromes (PHTS) and is characterized by autosomal dominant mutations of the PTEN tumor suppressor gene. The presence of hamartomas is the defining clinical feature. PHTS have increased risk of both benign and malignant tumors, including colon cancers. However, some patients with mutations do not show clinical signs and symptoms, while patients with the clinical syndrome may not have detectable PTEN mutations.
Case Description: A 43-year-old man with macrocephaly and multiple trichilemmomas was diagnosed with benign nasopharyngeal mass, presenting as recurrent epistaxis. The patient was suspected to have PHTS hence referred for endoscopy to evaluate presence of gastrointestinal hamartomas. EGD revealed multiple esophageal glycogenic acanthosis. Colonoscopy showed innumerable polyps in the cecum, ascending and sigmoid colon. Biopsies of the polyps yielded juvenile polyps, tubular adenoma, and tubulovillous adenoma. No family member is suspected or confirmed to have PHTS. Based on the current National Comprehensive Cancer Network (NCCN) operational diagnosis of PHTS, patient satisfied three major criteria: macrocephaly, multiple trichilemmomas, and multiple juvenile polyps. Patient was then advised for colonoscopy every 2 years due to increased risk of developing colorectal cancer approximately 9 to 20%. Genetic counseling was advised for both the patient and family members.
Discussion: PTEN gene mutations have poor penetrance. Even if PTHS is an autosomal dominant genetic condition, it may not be reliably detected in their family history. Screening colonoscopy is recommended because of increased risk of developing colorectal cancer. Moreover, annual comprehensive examinations are recommended for cancer surveillance.
PP-02-020
A Rare Case of Pancreatic Adenocarcinoma presenting as Colon mass: A Case Report
Pamela Bianca Yap and Maria Angelica Franco
St.lukes medical center, Quezon city, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Pancreatic adenocarcinoma is one of the most aggressive forms of malignancy. It commonly presents as abdominal pain and pancreatic duct obstruction. The lack of early symptomatology leads to delay in diagnosis with a 5-year survival rate of 8% in the advanced stage. It usually metastasizes in the liver, lymph nodes and peritoneum however, rarely metastasizes to the colon with only few reported cases. Patients have poor prognosis averaging 7 months after detection of colon metastasis.
Case Description: Patient is a 52/M, heavy smoker presenting with crampy abdominal pain and 10kg weight loss 2 months prior to index admission. There was no reported changes in bowel movement or gastrointestinal bleeding. On physical examination, he is cachectic with diffuse abdominal tenderness. Colonoscopy was done showing a polypoid easily friable 2cm mass at the cecum. Biopsy showed adenocarcinoma with mucinous features which is not typical of primary colon cancer. CT scan of the whole abdomen with IV contrast revealed a gastric antral mass and peritoneal carcinomatosis. The pancreas was unremarkable. Further workup with upper endoscopy, upper EUS and exploratory laparotomy with peritoneal sampling all revealed metastatic carcinoma. Immunohistochemical(IHC) of the cecal mass showed metastatic carcinoma favoring a pancreatic vs. gastric origin. Subsequent additional IHC eventually revealed adenocarcinoma compatible with pancreaticobiliary origin.
Discussion: Differentiating colon metastases from primary colon cancer can be challenging. When dealing with metastatic cancers, IHC can help identify the tissue of origin by detecting markers that are typical of specific primary tumors. This is crucial for guiding treatment decisions.
PP-02-021
“Hookworm-Induced Gastrointestinal Hemorrhage: A Case Study on Necator americanus"
Meutia Yasrizal1 and Muhammad Firhat Idrus1
1Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;2Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Hookworm infections in humans are typically caused by the helminth nematodes Necator americanus and Ancylostoma duodenale.1 Most infected individuals remain asymptomatic, with iron deficiency anemia resulting from chronic blood loss in the digestive tract 2
Case Description: In this report , we discuss the case of a 64-year-old male patient presented with recurrent black stools for the past 6 months before hospital admission. The patient frequently visited the hospital due to weakness, and upon examination, his hemoglobin (Hb) levels were consistently low, requiring blood transfusions.
Laboratory results showed Hb 3.6 g/dL, hematocrit 13.8%, leukocytes 7210/uL, platelets 372,000/uL, MCV 77.5 fL, and MCH 20.2 pg. Differential count showed basophils at 0.1%, eosinophils at 11.3%, neutrophils at 67.9%, lymphocytes at 8.9%, and monocytes at 11.8%.
Complete feces examination revealed brown color, leukocytes 2-3 per high power Aield (HPF), erythrocytes 1-2 per HPF, and positive bacteria.
Colonoscopy results showed a normal colon. EGD results revealed a hiatal hernia and helminthiasis. Stool parasitology results showed hookworm eggs and adult Necator americanus.
PP-02-022
Phlebosclerotic colitis with chronic Traditional Chinese Medicine use: A case report
Pun Fung Yip, Wing Chee Lai and Steven Woon Choy Tsang
Tseung Kwan O Hospital, Hong Kong
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Phlebosclerotic colitis (PC) is different from ordinary ischemic colitis with distinct radiological, endoscopic and histopathological features. Around 200 cases were reported until 2020, mainly affecting the Asian population. Possible etiologies include portal hypertension, right-sided heart failure, diabetes mellitus, vasculitis, hemodialysis and herbal use.
Case description: A 59-year-old lady presented with abdominal pain and diarrhea. She took Traditional Chinese Medicine (TCM) for 10 years. CT showed venous calcification and sclerosis in ascending and transverse colonic mesentery, with colonic thickening and edema, suggestive of venous ischemic colitis. Colonoscopy showed dark-purple mucosa at ascending and transverse colon. Biopsies show hyalinization and fibrosis in lamina propria, with hyalinized and thickened walls at capillary and submucosal veins. Findings are suggestive of PC. Tumour markers, rheumatological and hematological workup are unremarkable. Gardenia jasminoides was identified in TCM, a possible cause of PC.
Patient improved with oral mesalazine and discontinued TCM. Follow-up CT at 1 year showed similar calcification at mesenteric veins.
Discussion: A review analyzed herbal ingredients taken by 25 PC patients, all identified Geniposide. Geniposide is the major constituent of Gardenia fruits, commonly used in Kampo and TCM. Another study showed a positive correlation between the quantity of Gardenia-containing Chinese medical liquors consumption and the CT severity of PC.
PP-02-023
A successful endoscopic banding without resection for neuroendocrine tumor in duodenal bulb: a case report
Ahmad Fariz Malvi Zamzam Zein1,2, Hani Andriani3, Nunik Royyani4, Rabbinu Rangga Pribadi5 and Bethy Suryawathy Hernowo6
1Division of Gastroenterohepatology, Department of Internal Medicine, Fatmawati Central General Hospital, South Jakarta, Indonesia;2Department of Internal Medicine, Faculty of Medicine, Universitas Swadaya Gunung Jati - Waled General Hospital, Cirebon, Indonesia;3Department of Anatomical Pathology, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;4Department of Radiology, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia;5Division of Gastroenterology, Pancreatoobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia - Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia;6Department of Anatomical Pathology, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Duodenal neuroendocrine tumor (d-NET), a rare gastrointestinal tumor, remains challenge especially in limited settings. Safe and effective endoscopic management is preferable.
Case Description: A 39-year-old male patient admitted to primary hospital with dyspepsia. Esophagogastroduodenoscopy identified duodenal bulb mass with central depression bulb (Fig 1a) and irregular microsurface and microvascular pattern on NBI (Fig 1b). Abdominal CT scan revealed 16-mm solitary duodenal mass with both arterial and venous phases enhancement and no lymph involvements or metastasis were detected. Histopathological findings revealed monotonous regular cells with round-and-oval nuclei and salt-and-pepper chromatin. Immunohistochemistry test showed positive for chromogranin and synaptophysin, negative for S-100, and Ki67 approximately <3%. Diagnosis of well-differentiated d-NET (G1) was confirmed.
EBWR was performed uneventfully without clinically active bleeding and pain post-EBWR. No adverse event was reported during 3-month evaluation visits.
Esophagogastroduodenoscopy at 3-month follow-up showed small wall-thickening at post-EBWR site with regular pattern on NBI view. Histopathological and immuhistochemistry finding confirmed negative for recurrence. Biennial surveillance esophagogastroduodenoscopy-biopsy was suggested in this case.
Discussion: Histopathological features and positive for chromogranin and synaptophysin confirmed well-differentiated d-NET, while Ki-67 index <3% constituted low-grade (G1).
Endoscopic management is recommended in low-grade dNETs. Endoscopic resection is merely considered in advanced centers with carrying risk of complications. EBWR is preferred technique providing considerable effectiveness, feasible in any setting, almost nil complication, and inexpensive. This case reported safe and complete eradication of d-NET (G1) using EBWR.
Regarding the risk of recurrence, subsequent esophagogastroduodenoscopy-biopsy are recommended. Strategy for surveillance is enhanced.
PP-02-024
Rectal endometriosis biopsy targeted by magnification endoscopy narrow-band imaging (ME-NBI) and endoscopic ultrasonography (EUS)
Hongbin Zhu, Ling Hu, suJuan Zhang and Yumeng Chen
Department of gastroenterology and hepatology, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Ectopic endometriosis is characterized by the presence of endometrial tissue outside the uterine cavity, leading to irritation, bleeding, and inflammation. In this case, we employed biopsy targeted by ME-NBI plus EUS and established the definitive diagnosis of rectal endometriosis.
Case description: A 43-year-old female presented with intermittent abdominal pain and hematochezia. Colonoscopy revealed a broad-based, flat-elevated lesion in the sigmoid colon. The mucosal surface of the lesion exhibited uneven granularity, marked congestion, and edema (Figure A). ME-NBI examination revealed type II glandular openings, characterized by sparse microvascular density, accompanied by elongated and thickened blood vessels. Additionally, white opaque substances were observed within the glands (Figure B). EUS examination showed the disappeared stratification and significant thickeness of the wall. A buffalo horn-shaped, hypoechoic, and homogeneous pelvic mass measuring 24.5 × 24.0 mm was detected involving the posterior cervical wall and extending into the mucosal layer of the rectal wall (Figure C). A biopsy of the granular area revealed chronic inflammation with erosion in the rectal mucosa, as well as endometriosis in the lamina propria (Figure D). A gonadotropin-releasing hormone agonist (Leuprorelin) was administered via subcutaneous injection to induce atrophy of the endometriotic tissue and uterine fibroids. This treatment resulted in significant alleviation of the patient’s symptoms.
PP-02-025
A Case of lethal Mediastinitis after Intramural Esophageal Hematoma
1Department of respiratory medicine, the 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China;2Department of Pulmonary and Critical Care Medicine, the fourth Medical Center of Chinese PLA General Hospital, Beijing, China;3Department of gastroenterology and hepatology, the 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Intramural Esophageal Hematoma (IEH) arises from acute mucosal and submucosal injury, leading to blood accumulation in the esophageal wall. It is typically considered a benign disease with a generally favorable prognosis. Herein, we report a case of IEH complicated by subsequent letheal mediastinitis.
Case description: A 53-year-old woman presented to our hospital with dysphagia for 10 days. The dysphagia onset accompanied by severe retrosternal pain and recurrent vomiting began following the ingestion of tablets 10 days prior. CT revealed diffuse thickening of the esophagus wall with fluid retention in the lumen and right-sided pleural effusion. Endoscopic examination identified a stenosis of the esophagus 25-35 cm from the incisors, along with a 1 cm-wide, strip-shaped, dark red elevation, bleeding easily (Figure A). Two days later, CT showed free air around the lower esophagus and below the diaphragm, with an increase in pleural effusion (Figure B). Thoracentesis yielded 250 ml of turbid, bloody pleural fluid. Her symptoms ameliorated following symptomatic treatment. However, two weeks later, the patient experienced episodic dyspnea, which progressively worsened, accompanied by neck swelling and coagulation dysfunction. CT confirmed a diagnosis of mediastinitis (Figure C). Regrettably, her condition progressively deteriorated and she died 35 days after initial presentation.
Discussion: Based on the clinical manifestations, imaging and laboratory tests, we suspect the patient succumbed to pulmonary hypertension associated with fibrotic mediastinitis and subsequent right heart failure. This case underscores the importance of early diagnosis of IEH and, more significantly, elucidates the potential for IEH to progress to fatal mediastinitis.
PP-02-026
Early detection and management of acute superior mesenteric vein thrombosis following variceal hemostasis
Hongbin Zhu and Ling Hu and Jing Li and Jiana Li and Lili Liu and Zhoupei Fan and Qinqin Xiao
Department of gastroenterology and hepatology, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China;Department of Respiratory Medicine, The 983rd Hospital of Joint Logistic Support Force of People's Liberation Army, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Superior mesenteric venous thrombosis (SMVT) is a rare condition but portends a mortality rate of up to 23%. SMVT subsequent to endoscopic hemostasis for variceal bleeding in patients with cirrhosis are reported rarely. Here, we illustrate the management of such a rare condition.
Case Description: All four patients presented with hematemesis and melena. Abdominal CT scans did not reveal any signs of mesenteric thrombosis. Approximately five days post-procedure, following endoscopic variceal ligation (EVL) and/or endoscopic injection sclerotherapy (EIS) for hemostasis, the patients reported different degrees of abdominal discomfort, bloating, and loss of appetite postprandially. Laboratory test indicated a significant increase in D-dimer levels, reaching a peak of 28 mg/L. The formation of SMVT was confirmed through enhanced abdominal CT scans or ultrasound. Following the subcutaneous administration of low molecular weight heparin (LMWH), the symptoms were markedly relieved and D-dimer levels decreased. Following their discharge, patients were administrated a daily oral dosage of 10 mg rivaroxaban. Regular clinical follow-ups were conducted combined with abdominal ultrasounds every four weeks. None of the patients exhibited any progression of thrombus. Notably, the thrombus had almost completely disappeared in one of those patients after 3 weeks.
Discussion: EVL/EIS for variceal bleeding may potentially serve as a risk factor for acute SMVT. Thus, attention should be paid to the appearance of symptoms and elevation in the levels of D-dimer. The early commencement and sequential implementation of anticoagulation therapy appear to be both safe and effective. Abdominal ultrasound offers a cost-effective approach for monitoring SMVT.
PP-02-027
Efficacy and safety of endoscopic ultrasound-guided biliary drainage in managing malignant hilar biliary obstruction
1Gifu University Hosopital, Gifu, Japan;2Gifu Prefectural General Medical Center, Gifu, Japan;3Gifu Municipal Hospital, Gifu, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
ERCP-guided biliary drainage (BD) has been employed as the primary intervention in managing malignant hilar biliary obstruction (MHBO). Recently, EUS-BD has emerged as a useful drainage technique; however, its efficacy in MHBO remains insufficiently explored.
Aims: To assess the efficacy and safety of EUS-BD in MHBO.
Methods: Patients who underwent EUS-BD for MHBO from 2012 to 2023 were retrospectively reviewed. The study outcomes were the technical success and clinical success rates, and safety.
Results: 28 patients with MHBO were included with etiologies of bile duct cancer in 17, gallbladder cancer in 2, and metastatic lesions in 9. The obstruction types were Bismuth Type 2 in 9, Type 3A in 7, and Type 4 in 12. EUS-BD was performed as initial drainage in 10 and rescue drainage (s/p ERCP-BD) in 18. The technical success rate was 100% (28/28), with stent placement configuration of hepaticogastrostomy alone in 21, HGS with bridging (left to right system) in 3, antegrade stenting (AG: stenting at the obstruction) alone in 2, AG with bridging in 1, and AG with HGS in 1. The clinical success rate was 82% (23/28). Adverse events were observed in 3 (11%), all related to bile peritonitis. Comparisons of clinical failure with various clinical factors showed significant differences in Bismuth Type 4 and drainage area of less than 3 segments.
Conclusion: EUS-BD is considered useful in the management of MHBO. However, careful consideration is required for its indication, as the clinical success rate was not exactly high. Further studies are warranted.
PP-02-028
The Influence of Intravenous Antispasmodics on Gastric Transit Time in Capsule Endoscopy
Dabin Kim
Gyeongsang national university hospital/Gastroenterology, Jinju-si, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: This study investigates the impact of intravenous (IV) antispasmodics on gastric transit time in capsule endoscopy and explores the influence of various risk factors on this transit time.
Methods: Data from patients who underwent capsule endoscopy were analyzed. Patients were divided into two groups: those who received IV antispasmodics (Buscopan) on the same day as the procedure and those who did not receive IV antispasmodics. Additionally, we evaluated the impact of risk factors (alcohol, smoking, diabetes, hypertension) on gastric transit time. Statistical analysis, including t-tests, was performed to compare the mean transit times and assess the significance of differences.
Results: A significant difference in gastric transit times was observed between the groups. Patients who received IV antispasmodics on the same day had a mean gastric transit time of 149.00 hours (SD = 200.94), while those who did not had a mean gastric transit time of 52.55 hours (SD = 63.49) (t = 2.543, p = 0.014). Risk factor analysis showed no statistically significant differences in gastric transit times for alcohol (p = 0.819), smoking (p = 0.854), diabetes (p = 0.873), and hypertension (p = 0.421).
Conclusion: The use of IV antispasmodics is associated with prolonged gastric transit time in capsule endoscopy. Risk factors such as alcohol, smoking, diabetes, and hypertension do not significantly affect gastric transit time. These findings underscore the importance of considering pre-procedural medications when interpreting capsule endoscopy results and suggest that further research is needed to optimize clinical protocols.
PP-02-029
Urinary Lipoarabinomannan (LAM) -A simple test that aids in differentiating Intestinal Tuberculosis from Crohn's Disease
Mukesh Singh and MANJEET Goyal and Himanshu Narang and Malambo Mubbunu and Peeyush Kumar and Bhaskar Kante and Sudheer Vuyyuru and Srikant Mohta and Ashish Dutt Upadhyay and Prasenjit Das and Ankur Goyal and Raju Sharma and Govind Makharia and Saurabh Kedia and Vineet Ahuja
Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: To evaluate the diagnostic accuracy of urinary LAM in distinguishing Intestinal Tuberculosis (ITB) from Crohn's Disease (CD) among patients presenting with clinical features of these conditions.
Methods: A prospective study was conducted at a tertiary health care center from January 2021 to April 2022. The study included patients diagnosed with ITB, CD, or indeterminate conditions. Comprehensive evaluations, laboratory investigations, CT enterography, colonoscopy, and histopathological analyses were performed. Fresh morning midstream urine samples were collected and analyzed using the Alere Determine TB LAM Ag kit. The analytical team was blinded to clinical data. Statistical analysis determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall diagnostic accuracy of urinary LAM.
Results: Among 98 patients, 36 were diagnosed with ITB and 62 with CD. Urinary LAM was positive in 9 out of 36 ITB patients, yielding a sensitivity of 25% (95% CI 12.12%-42.20%) and a PPV of 100% (95% CI 66.37%-100.00%). None of the CD patients tested positive for urinary LAM, resulting in a specificity of 100% (95% CI 94.22%-100.00%) and an NPV of 69.66% (95% CI 65.54%-73.50%). The overall diagnostic accuracy of urinary LAM in differentiating ITB from CD was 72.45%. Adding urinary LAM testing to existing diagnostic criteria improved accurate identification of ITB from 44% to 57%.
Conclusion: Urinary LAM testing exhibits high specificity and PPV, making it a significant adjunct in the diagnostic process for ITB.
PP-02-030
Structured Yoga Practices significantly improve Quality of Life in Ulcerative Colitis: a randomized controlled trial
Janaki D B Sankar Kalum1, Himanshu Narang1, Akriti Shukla3, Govind K Makharia1, Saurabh Kedia1, Peeyush Kumar1, David Mathew Thomas1, Shubham Prasad1, Malamboo Mubbunu1, Shubhi Virmani1, Divya Madan1, Mahak Verma1, Gautam Sharma2 and Vineet Ahuja1
1Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi NEW DELHI, India;2Department of Cardiology and Professor In-charge, Center for Integrative Medicine and Research (CIMR), All India Institute of Medical Sciences, New Delhi NEW DELHI, India;3Centre for Integrative Medicine and research, AIIMS, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Objective: There are anecdotal studies on effect of Yoga in patients with ulcerative colitis (UC). We investigated the effect of yoga on health-related quality of life and disease activity in UC patients in remission.
Materials and Methods: Patients with UC in remission (SCCAI <3 and fecal calprotectin<150 μg/mg) were randomly assigned to either IBD-specific yoga therapy (45 minutes/day, five days/week) or brisk walking (150 minutes/week), in addition to standard medical treatment, for 6 months. Primary outcome was the effect of yoga on quality of life using the World Health Organization Quality of Life – BREF (WHOQOL-BREF), Hamilton Anxiety Rating Scale (HAM-A), and Perceived Stress Scale (PSS) questionnaires at 6 months. Secondary outcome was relapse rates at 6 months.
Results: 100 patients were randomized with 50 in each arm and 5 were lost to follow up. Baseline clinical and demographic characteristics and WHOQOL BREF, PSS and HAM-A scores were similar. At 6 months, patients in yoga group showed significantly lower PSS (16.70±1.44 vs. 17.34±1.5, p=0.03) and anxiety scores (1.51±0.68 vs. 1.89±0.79, p=0.01) compared to walking. Yoga also significantly improved physical (71.29±2.94 vs. 69.65±3.4, p=0.014) and psychological well-being (71.55±4.28 vs. 68.34±4.77, p=0.001), with similar social scores (63.85±6.98 vs 63.47±6.68 p=0.79). One patient in each group had disease relapse.
Conclusion: Yoga can be considered an effective ancillary intervention to improve the quality of life in patients with UC in remission.
PP-02-031
Medication Non-Adherence in Inflammatory Bowel Disease Patients in a resource-constrained setting
Md Yasir Arafat, M Masudur Rahman, Rashedul Hasan and Md Iqbal Hossain
Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Since drug adherence is an important issue in maintaining remission in inflammatory bowel disease (IBD) in a resource-constrained country like Bangladesh, we planned to conduct a study to determine the drug non-adherence rate, predicting factors, and the reason associated with non-adherence.
Materials and Methods: This prospective cross-sectional study was done at Sheikh Russel National Gastroliver Institute and Hospital, Bangladesh. The frequency of missed doses was evaluated based on recall of drug adherence from the previous two weeks. Medication intake of <80% of the advised dose was defined as non-adherence.
Results: A total of 272 patients [94(34.6%) Ulcerative colitis (UC) and 178(65.4%) Crohn’s disease (CD)] were included. The mean age was 32.1±10.6 years, Male: 174(64%), median disease duration: 2.0±2.07 years. The overall rate of non-adherence was 46% [38/94(40.4%) UC and 87/178(48.9%) CD]. Univariate analysis identified urban residency and longer disease duration as predicting factors associated with non-adherence. Age, sex, marital status, education and IBD types were not significant predictors (Table 1). Similarly, multivariate analysis revealed longer disease duration (AOR 1.14, CI 1.00-1.30, P=0.04) and urban residency (AOR 0.54, CI 0.31-0.94, P=0.03) as significant predictors of non-adherence. The main reasons for non-adherence were forgetfulness 43(34.4%), unavailability of drugs 35(28%), felt better 28(22.4%), lack of adequate information 27(21.6%), cost of drugs 21(16.8%).
Conclusion: Almost half of the patients were non-adherent to their IBD medication. The key predictors of non-adherence were longer disease duration and urban residency. The most common reasons for non-adherence were forgetfulness and the unavailability of drugs.
PP-02-032
Knowledge, Attitudes and Practice about Vaccination in Inflammatory Bowel Diseases
Md Yasir Arafat, M Masudur Rahman, Azam Jahangir, Karzon Dey Sarker, Shahana Parvin, Md Kamrul Hasan and Md Musab Khalil
Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Although guidelines suggest vaccination against various infections in inflammatory bowel disease (IBD), the practice varies in different populations. Hence, we aimed to study vaccination rates and knowledge of and attitudes toward vaccination among a cohort of Bangladeshi IBD patients.
Materials and Methods: Consecutive IBD patients attending the IBD clinic at Sheikh Russel National Gastroliver Institute and Hospital, Bangladesh were interviewed with a standardised validated questionnaire on self-reported vaccination.
Results: A Total of 224 patients with IBD were included [male, 139 (62.1%); median age 32 years (interquartile range, 24.3–41)]. Of all, 78/224(34.8%) were ulcerative colitis (UC) and 146/224(65.2%) crohn’s disease (CD). Self-reported vaccine uptake was for hepatitis B virus 83/224(36.6%) [UC(22/78, 28.2%) CD(61/146, 41.8%),p=0.06]; seasonal influenza 78/224 (34.8%) [UC(18/78,23.1%) CD (60/146, 41.1%),p<0.01]; pneumococcus 76/224 (33.9%) [UC 17/78,21.8%), CD (59/146,40.4%),p<0.01]; measles, mumps and rubella (MMR) 183/224(81.7%) [UC(71/78,91%), CD(112/146,76,7%),p=0.01]; diphtheria, pertussis and tetanus (DPT) 183/224 (81.7%) [UC(70/78,89.7%, CD(113/146,77.4%),p=0.03]; Varicella 0/224(0%); Human papilloma vaccine (HPV) 5/85 (5.9%).
Overall, only 26.8% were vaccinated against three or more adult vaccines. Use of steroid (AOR [adjusted odds ratio] 15.6, 95% confidence interval [CI] 6.3–38.5, P<0.001), immunosuppressive (AOR 6.1, 95% CI 2.5– 15, P<0.001), and biological agents (AOR 20, 95% CI 2.8–139.8, P=0.002) were associated with at least three or more adult vaccines. The major reasons for non-vaccination were not knowing the presence of the vaccine 116/224(51.8%), necessity of the vaccine 117/224(52.2%) and non-affordability 50/224(22.3%)(Table 1).
Conclusion: Adult vaccination rates were suboptimal for patients with IBD. Lack of Knowledge and awareness are the main reasons for non-vaccination.
PP-02-033
Incidence, risk factors, and outcomes of chronic antibiotic-refractory pouchitis in Korean patients with ulcerative colitis
Ji Eun Baek1,3, Jung-Bin Park1, June Hwa Bae1, Min Hyun Kim2, Seung Wook Hong1, Sung Wook Hwang1, Jong Lyul Lee2, Yong Sik Yoon2, Dong-Hoon Yang1, Byong Duk Ye1, Jeong-Sik Byeon1, Seung-Jae Myung1, Chang Sik Yu2, Suk-Kyun Yang1 and Sang Hyoung Park1
1Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea;2Division of Colon and Rectal Surgery, Department of Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea;3Department of Gastroenterology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background/Aims: The study investigated the incidence, risk factors, and clinical outcomes of chronic antibiotic-refractory pouchitis (CARP) in Korean patients with ulcerative colitis (UC).
Methods: This single-center retrospective study included patients with UC who underwent total proctocolectomy with ileal pouch-anal anastomosis (IPAA) at the Asan Medical Center in Korea between January 1987 and December 2022. Primary outcomes were endoscopic remission and pouch failure. Cox’s proportional hazard model was used to identify risk factors of CARP.
Results: A total of 232 patients were analyzed. The most common cause of surgery was steroid refractoriness (50.9%), followed by dysplasia/colorectal cancer (26.7%). Among 74 patients (31.9%) with chronic pouchitis (CP), 31 (13.4%) had CARP, and 43 (18.5%) had chronic antibiotic-dependent pouchitis (CADP). The most frequent endoscopic phenotype was focal inflammation of the pouch (CP, 47.3%; CARP, 35.5%; CADP, 55.8%). Patients with CARP were less likely to have concomitant probiotics compared with CADP (29.0% vs 72.1%; p<0.01). The endoscopic remission rate in CP, CARP, and CADP was 14.9%, 9.7%, and 18.6%, respectively. The pouch failure rate in CP, CARP, and CADP was 13.5%, 16.1%, and 11.6%, respectively. Current smoking status (adjusted Hazard Ratio [aHR] 2.96; 95% confidence interval [CI] 1.27–6.90; p=0.01) and previous use of biologics/small molecules (aHR 2.40; 95% CI 1.05–5.53; p=0.04) were significantly associated with CARP development.
Conclusions: Current smoker and previous user of biologics/small molecules in UC patients who underwent IPAA had a higher risk of CARP. Concomitant use of probiotics was less likely to be associated with developing CARP.
PP-02-034
Efficacy and Safety of Vedolizumab-Tofacitinib(VETO) combination in Ulcerative-Colitis refractory to Anti-TNF and second-line advanced therapy
Rupa Banerjee, Pardhu Bharath Neelam, Dhanush Mekala, Nalini Raghunathan and Rajendra Patel
AIG Hospitals, Hyderabad, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Management of refractory Ulcerative colitis (UC) not responding to multiple advanced therapies is challenging. Role of combination therapy in management of refractory UC is evolving. We studied safety and efficacy of Vedolizumab and Tofacitinib combination(VETO) therapy in anti-TNF refractory patients unresponsive to either Vedolizumab or Tofacitinib alone.
Materials and methods: We prospectively recruited consecutive UC patients who were anti-TNF non-responsive after consent. They were randomised to either Tofacitinib or Vedolizumab. Non-responders with second-line agents were given VETO therapy and followed for 24 weeks(Figure1). Clinical response (week 8), clinical and endoscopic remission rates (week 24) and need for colectomy were assessed. All adverse events were recorded. Paired t-test was used to compare the mean Mayo scores at the baseline and end of the follow-up.
Results: Of 91 anti-TNF refractory patients, Vedolizumab and Tofacitinib were given as second-line therapy to 38 and 40 respectively. Twenty-four non-responders and partial responders (mean40.8±11.7yrs, females 63%) received VETO therapy. At week 8, 5(20.8%) and 18(75%) achieved clinical remission and response respectively. At week 24, 17(70.8%), 14(58.3%), 7(33.3%) achieved clinical response, remission and endoscopic remission respectively. Four(16.6%) required steroid rescue therapy. Three(12.5%) required colectomy by the end of 8 weeks(Figure1). Compared to baseline, Mean difference in Mayo score at 24-weeks is 5.33±0.53(p<0.01). Two developed pseudomembranous colitis which responded to vancomycin. No severe adverse events were noted.
Conclusion: The VETO combination appears to be an effective and safe option for the management of refractory UC with more than 50% achieving clinical remission at 24weeks.
PP-02-036
Plant-based diets mitigate the incidence risk, disease course and comorbidity of inflammatory bowel disease
Jie Chen1, Yuhao Sun1,3, Lintao Dan1,2, Judith Wellens4,5, Shuai Yuan6, Hong Yang7, Tammy Tong8, Amanda Cross9, Nikolaos Papadimitriou10, Antoine Meyer11, Christina Dahm12, Susanna Larsson6,13, Alicja Wolk6, Jonas Ludvigsson14,15, Kostas Tsilidis16,17, Edward Giovannucci18,19, Jack Satsangi5, Xiaoyan Wang1, Evropi Theodoratou20,21, Simon Chan22,23 and Xue Li2
1Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, China;2Department of Big Data in Health Science School of Public Health and The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;3Department of Gastroenterology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;4Department of Chronic Diseases and Metabolism, Translational Research Centre for Gastrointestinal Disorders (TARGID), KU Leuven, Leuven, Belgium;5Translational Gastroenterology Unit, Nuffield Department of Medicine, Experimental Medicine Division, University of Oxford, John Radcliffe Hospital, Oxford, UK;6Unit of Cardiovascular and Nutritional Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden;7Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China;8Cancer Epidemiology Unit, Nuffield Department of Population Health, University of Oxford, Richard Doll Building, Old Road Campus, Oxford, UK;9School of Public Health and Department of Surgery and Cancer, Imperial College London, London, UK;10Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France;11INSERM, Centre for Research in Epidemiology and Population Health, Institut Gustave Roussy, Université Paris Saclay, Villejuif, France;12Department of Public Health, Aarhus University, Bartholins Allé 28000, Aarhus, Denmark;13Department of Surgical Sciences, Uppsala University, Uppsala, Sweden;14Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden;15Department of Pediatrics, Orebro University Hospital, Orebro, Sweden;16Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London, UK;17Department of Hygiene and Epidemiology, University of Ioannina School of Medicine, Ioannina, Greece;18Department of Epidemiology, Harvard T.H. Chan School of Public Health, Massachusetts, USA;19Department of Nutrition, Harvard T.H. Chan School of Public Health, Massachusetts, USA;20Centre for Global Health Research, Usher Institute, University of Edinburgh, Edinburgh, UK;21Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK;22Department of Gastroenterology, Norfolk and Norwich University Hospital NHS Trust, Norwich, UK;23Department of Medicine, Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich, UK
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the relationship between plant-based diets and the incidence and course of inflammatory bowel disease (IBD) across two large prospective cohorts.
Materials and Methods: Data from the UKB including 187,888 participants free of IBD and 2,133 IBD patients and the EPIC cohort including 341,539 individuals free of IBD. The plant-based diet indexes (healthy and unhealthy PDI) were constructed based on the 24-h dietary recalls and food frequency questionnaires. Cox regression was applied to estimate hazard ratios (HRs), and interactions between genetics and plant-based diets were explored.
Results: Over a median follow-up of 11.6 years in the UKB, 925 new IBD cases were recorded. Higher adherence to a healthy PDI was linked to a lower IBD risk (HR 0.75, 95% CI 0.60-0.94), while an unhealthy PDI was associated with increased risk (HR 1.48, 95% CI 1.21-1.82). Among individuals with established IBD, we observed significant associations of healthy PDI (HR 0.50, 95% CI 0.30 to 0.83) and unhealthy PDI (HR 2.12, 95% CI 1.30 to 3.44) with IBD-related surgery. In the EPIC cohort, similar estimation and trends were observed with 548 incident cases over a median follow-up of 14.5 years, with HRs of 0.71 (95% CI 0.59-0.85) for healthy and 1.54 (95% CI 1.30-1.84) for unhealthy PDI. The diet-IBD association was partially mediated by inflammation and more pronounced in genetically susceptible individuals.
Conclusion: Adherence to a healthy plant-based diet may reduce the risk and improve outcomes for IBD, especially in those with higher genetic risk.
PP-02-037
Identification of highly-virulent strain from inflammatory bowel disease and characterizing the pathogenicity at transcriptome level
Jingyi Chen, Congyi Yang, Yalan Xu, Yuzhen Zhao, Jushan Wu, Yulan Liu and Ning Chen
Peking University People's Hospital, Beijing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We characterize the pathogenicity differences of potential pathogens isolated from the saliva of inflammatory bowel disease (IBD) patients at the transcriptome level.
Materials And Methods: Streptococcus mutans (S.m) strains were isolated from the saliva of IBD patients with different disease phenotypes. Differential gene expression, module clusters, protein-protein interaction, function annotation and enrichment was used to characterize the pathogenicity of S.m at the transcriptome level.
Results: PCA analysis showed that there were differences between pathogenic and non-pathogenic strains at the transcriptome level. Pathogenic strains had 240 up-regulated and 178 down-regulated genes compared to non-pathogenic strains. We identified 395 virulence genes in S.m isolates and obtained 80 overlapped genes after comparing virulence genes with differential genes. The protein-protein interaction (PPI) network was constructed and identify the top 10 hub genes with the highest connectivity. The module consisting of the most closely interacting genes were identified and 5 key node genes (SMU_833, gtfD, luxS, spaP, rgpE) were obtained after comparing hub genes with module genes. In addition, differential genes of pathogenic/non-pathogenic isolates were enriched in functional pathways which is closely related to carbohydrate metabolism. Therefore, 45 carbohydrate active enzyme-related genes were identified and sacB, rgpE, SMU_833 were identified as key nodes in the PPI network.
Conclusion: This study is the first to find that S. m isolates from UC patients have different pro-inflammatory effects. Identifying virulence genes of potential oral pathogens at the transcriptome level is helpful to reveal the mechanisms of different disease development pattern in IBD.
PP-02-038
Ustekinumab for the management of Crohn’s disease: Experience from Middle-East
Thomas Cherukara, Noble Thomas, Yousif Al Serkal and Amit Kumar Dutta
Sharjah, Emirates Health Services, Kuwaiti Hospital, Sharjah, United Arab Emirates
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Ustekinumab is approved for therapy of moderate to severe Crohn’s disease. We report the efficacy and safety of this drug in management of a mixed group (prior biological therapy and treatment naïve) of patients with Crohn’s disease.
Methodology: Patients with a diagnosis of Crohn’s disease and treated with Ustekinumab from 2019 to 2023 were retrospectively recruited. The clinical, demographic and investigation details were recorded on a structured data collection form. The details of therapy with Ustekinumab, response to therapy and therapy prior to Ustekinumab use were noted. The primary aim was the proportion of patients achieving clinical and biochemical remission (normalization of CRP or fecal calprotectin) after starting treatment.
Results: Eighteen patients were included in this study. Table 1 shows the clinical features of the study subjects. Five patients had received prior biological therapy. The median fecal calprotectin value was 1000 microgram/gram and mean CRP value was 37+31mg/L before treatment. The median duration of follow-up was 15.5 months (range 3-44 months). Nine out of the eighteen patients achieved the primary end point after a median period of 6 months (range 3-12). Three patients improved clinically but did not achieve the primary end point. In two non-responders, the frequency of therapy was increased to every 4 weeks but was not effective. Serious adverse effect was noted in one patient who developed infected abdominal collection.
Conclusions: Ustekinumab therapy in Crohn’s disease (prior biological therapy and treatment naïve) is generally safe and leads to clinical and biochemical remission in 50% of patients.
PP-02-039
Enhancing the predictions of cytomegalovirus infection in severe ulcerative colitis using deep learning ensemble model
A Reum Choe1, Jeong Heon Kim2, Ju Ran Byun1, Yehyun Park1, Eun Mi Song1, So Hyun Ahn3 and Sung Ae Jung1
1Ewha Medical University Hospital, Seoul, South Korea;2Department of Medicine, Yonsei University College of Medicine, South Korea;3Ewha Medical Artificial Intelligence Research Institute, Ewha Womans University College of Medicine, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Cytomegalovirus (CMV) reactivation is common among patients with severe ulcerative colitis (UC), resulting in poorer prognoses than patients without CMV reactivation. The principal diagnostic approach for CMV involves biopsies, which are time-consuming and present challenges for early detection. To tackle this issue, our study utilizes deep learning to differentiate CMV from severe UC using endoscopic imaging, thereby enabling early CMV diagnosis.
Materials and Methods: In this study, we examined 86 endoscopic images employing an ensemble of deep learning models, notably Densenet 121 pre-trained on ImageNet, to discriminate between cases of UC with and without CMV complications. Extensive preprocessing and test-time augmentation (TTA) techniques were applied to enhance the effectiveness of the models. Evaluation of the models' performance included metrics such as accuracy, precision, recall, F1 score, receiver operating characteristic (ROC) curves, and AUC values, highlighting the potential of deep learning to improve non-invasive gastroenterology diagnostics.
Results: An ensemble of four models augmented with TTA demonstrated superior performance in classifying UC endoscopic images. It attained an accuracy of 0.83, precision of 0.83, recall of 0.91, and an F1-score of 0.87. These metrics underscore the ensemble's reliability and well-rounded performance. Particularly noteworthy is the substantial decline in performance metrics observed in models without TTA, highlighting the critical role of TTA.
PP-02-040
Appendectomy Alleviates Colonic Inflammation via Secondary Bile Acid-Induced Regulation of Lipid Metabolic Pathways
Feifei Chu1,2, Jie Xiong1 and Hailong Cao1
1Tianjin Medical University General Hospital, Tianjin, China;2Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The specific role of the appendix as both an immune organ and a reservoir for gut microbiota in inflammatory bowel disease(IBD) remains elusive. Recent clinical studies have demonstrated that appendectomy can alleviate ulcerative colitis(UC). However, the underlying mechanisms are still unclear. This study aims to comprehensively explore the precise regulatory mechanisms of appendectomy in the process of colonic inflammation.
Materials and Methods: Appendectomy was performed on C57BL/6 mice followed by induction of dextran sulfate sodium (DSS)-induced colitis. Changes in colon length, body weight, and DAI score were evaluated, qPCR, western blot, and immunofluorescence staining were employed to assess production of pro-inflammatory cytokines and the level of colonic mucosal barrier markers. Public databases were utilized for bioinformatics analysis. Transcriptomic, 16S rRNA, and metabolomic sequencing were conducted.
Results and Conclusion: Appendectomy increased colon length, body weight, and enhanced mucosal barrier integrity in mice with colitis, indicating alleviation of colitis. 16S rRNA sequencing indicated significant differences in microbial taxa such as Mucispirillum, Bacleroides, Clostridium_sensu_stricto_1 and Turcicibacter genus. Metabolomics analysis suggested that secondary bile acids accumulation. Both tanscriptome sequencing and metabolomics analysis enriched in lipid metabolism.
PP-02-041
Association of Psychological Distress and Resilience with Inflammatory Bowel Disease Activity and Quality of Life
Jaeyoung Chun and Yuna Kim and Jie-Hyun Kim and Young Hoon Youn and Hyojin Park
Yonsei University College Of Medicine, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We evaluated the association of psychological distress and resilience with HRQoL and disease activity in patients with IBD.
Materials and Methods: A total of 225 consecutive patients with Crohn’s disease (CD, n=108) and ulcerative colitis (UC, n=117) were enrolled at Gangnam Severance Hospital in Korea from 2021 to 2024. All participants completed self-reported questionnaires, including hospital anxiety and depression scale, perceived stress scale, the Korean resilience questionnaire, and the short IBD questionnaire. Disease activity was assessed using the Crohn's Disease Activity Index (CDAI) and the Harvey-Bradshaw index for CD, and the partial Mayo score and Simple Clinical Colitis Activity Index for UC, respectively.
Results: HRQoL was significantly poorer in active IBD compared to quiescent IBD. Poor HRQoL was significantly associated with anxiety (p<0.001), depression (p=0.018), and stress (p=0.002), but not resilience, in a dose-response relationship. Significant association of anxiety (p=0.010), depression (p<0.001), stress (p=0.005) with disease activity was detected in patients with CD, but not UC. Regardless of inflammatory biomarkers, disease activity was significantly associated with anxiety (p=0.050), depression (p<0.001) and stress (p=0.019) in patients with CD. In terms of CDAI components, subscores for general well-being were associated with psychological distress whereas stool frequency and abdominal pain were not.
Conclusion: HRQoL was significantly associated with psychological distress, but not resilience in patients with IBD. Psychological distress was associated with disease-related symptoms in patients with CD, particularly affecting general well-being. Physicians should be aware of the impact of psychological distress on patient-reported outcomes and HRQoL in the management of IBD.
PP-02-042
Muscle mass is preserved with dietary interventions in Crohn’s disease when using bedside muscle ultrasound
Jessica Fitzpatrick1, Peter R Gibson1, Daniel Clayton-Chubb1,2, Kirstin M Taylor2 and Emma P Halmos1
1Department of Gastroenterology, School of Translational Medicine, Monash University, Melbourne, Australia;2Department of Gastroenterology, Alfred Health, Melbourne, Australia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To measure and compare the performance of point-of-care techniques in measuring body composition in Crohn’s disease patients and evaluate the subsequent impact of two high-quality dietary interventions differing in emulsifier content but iso-caloric to habitual diet.
Material and Methods: In a double-blinded randomised controlled-feeding trial, adults with active Crohn’s disease received 4 weeks of either diet. Body composition assessments were completed at days 0 and 28, including dual energy X-ray absorptiometry (DXA, day 0 only), quantitative muscle ultrasound (QMUS), waist circumference, visceral fat via bioimpedance analysis (BIA) and hand-grip strength.
Results: In 24 participants (mean age 37 (95% CI 32-41) years, 12 male), myopenia was present on DXA at baseline in 50% women and 9% men, but all had normal BMI and none had abnormal hand-grip strength. 25% women and 36% men were osteopenic. Central adiposity was present in 50%. QMUS correlated well with DXA appendicular skeletal muscle mass (ASMI) (r=0.92; p<0.001), and QMUS-predicted ASMI demonstrated no bias and narrow limits of agreement (LOA) on Bland-Altman analysis (Figure). The mean difference of BIA ASMI vs DEXA ASMI was 7.15kg (LOA 2.3-12.0).The diets consistently reduced weight by 2.2 [1.3,3.2] kg (p=0.009), waist circumference by 4% (p<0.001) and visceral adipose tissue by 16% (p<0.001) without a change of QMUS (p=0.78).
PP-02-043
Effect of tofatinib and upadacitinib in the treatment of anti-TNF refractory ulcerative colitis
Maiko Ikenouchi and Hirokazu Fukui and Soichi Yagi and Koji Kaku and Yoko Yokoyama and Koji Kamikozuru and Mikio Kawai and Toshiyuki Sato and Norio Nishii and Kumiko Nakamura and Hirotsugu Eda and Yoshitaka Kitayama and Hiroo Sei and Takuya Okugawa and Toshihiko Tomita and Shinichiro Shinzaki
Hyogo Medical University, Nishinomiya, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Janus kinase (JAK) inhibitors including tofatinib (TOF) and upadacitinib (UPA) are frequently applied for patients with anti-TNF refractory ulcerative colitis (UC); however, their effects for those patients are remained unclear. Therefore, we investigated the effect of TOF and UPA on the clinical remission in patients with anti-TNF refractory UC.
Methods: The patients who had undergone anti-TNF therapy and thereafter received either TOF or UPA were enrolled. Primary outcomes were efficacy at 8 weeks after administration of TOF or UPA to those patients. Clinical remission was defined as partial Mayo Score (PMS) ≤2 and each subscore ≤1. Clinical response was defined as follows: the decrease of PMS from baseline is ≥2 and rectal bleeding subscore is ≤1 or its decrease from baseline is ≥1. Primary anti-TNF refractory was defined as failure to clinical remission by anti-TNF.
Results: Among 230 UC patients investigated, 170 received TOF and 60 received UPA treatment. The clinical response rate at 8 weeks after UPA treatment (81.7%) was higher than that after TOF treatment (66.5%). The clinical remission rate in UPA group (70.0%) was significantly greater than that in TOF group (44.7%) (OR, 2.87; 95%CI, 1.47-5.76). The clinical response rate in patients with primary anti-TNF refractory was 56.7% and 77.0% in TOF and UPA group, respectively. The clinical remission rate in those patients was significantly higher in UPA (77.0%) than in TOF (32.3%) group (OR, 3.3; 95%CI, 1.18-9.70).
Conclusions: UPA is may be a useful JAK inhibitor for the therapy of anti-TNF refractory UC.
PP-02-044
Safety and Efficacy of Allopurinol to Optimize Azathioprine Therapy in Inflammatory Bowel Disease: A Meta-Analysis
Ni Kadek Saras Dwi Guna
Faculty of Medicine, Udayana University, Bali, Badung, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Although thiopurines are used as conventional therapy in maintaining moderate-severe IBD remission, its hepatotoxicity due to high 6-MMP production and lack of efficacy remain unsolved. Recent studies have combined thiopurines with allopurinol to reduce hepatotoxicity and improve efficacy. This study aims to evaluate efficacy and toxicity after this combination therapy.
Materials and Methods: Systematic searches on Pubmed, Science Direct, and Google Scholar resulted in studies comparing IBD severity, hepatotoxicity, and bone marrow suppression before (thiopurine monotherapy) and after combination therapy. Review studies were excluded. The primary outcomes were 6-MMP, 6-TGN, and 6-MMP/6-TGN ratio. Secondary outcomes included ALT, AST, HBI Score, Partial Mayo Score, and WBC levels
Results: This study included 11 studies with total of 294 subjects that met the inclusion criteria. The pooled result showed that ALT and AST levels were significantly reduced after combination therapy (MD=-43.53; 95% CI=-62.06 to -25.00; p=0.00 and MD=-11.72; 95% CI=-17.11 to -6.33; p=0.00, respectively), supported by significantly lower 6-MMP level (MD=-8816.96; 95% CI=-10.004 to -7548.44; p=0.00). HBI and Partial Mayo scores were also lower after combination therapy (MD=-3.57; 95% CI=-4.05 to -3.08; p=0.00 and MD=-0.86; 95% CI=-1.44 to -0.28; p=0.00, respectively). However, the WBC level was significantly reduced after combination therapy (MD=-1.35; 95% CI=-2.47 to -0.23; p=0.00), due to significantly higher 6-TGN level (MD=136.25; 95% CI=102.63 to 169.88; p=0.00). The 6-MMP/6-TGN ratio was significantly decreased after combination therapy (MD=-67.61; 95% CI=-82.90 to -52.32; p=0.01).
Conclusion: Allopurinol-Thiopurine yielded significant hepatotoxicity and IBD severity reductions, yet associated with increased bone marrow suppression.
PP-02-045
Clinical Features of Ulcerative Colitis with Hypozincemia and Considerations for Zinc Replacement Therapy
Saiseikai Matsusaka General Hospital, Matsusaka city, Mie Prefecture, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To clarify the clinical features of ulcerative colitis with hypozincemia and the usefulness of Zinc replacement therapy.
Materials and Methods: Ninety-six UC patients, median age 51 years old, whose serum Zinc levels were measured between 2022 and 2024 were retrospectively enrolled. The Zinc <80 μg/dl was classified as hypozincemia group(LG), and Zinc ≥80 as normal group(NG). Twenty-eight patients in the LG who underwent Zinc supplementation were evaluated for disease activity after supplementation. Patient background (age, gender, extent of disease, relapse rate), CRP, F-cal, pMayo score, and Mayo score were examined.
Results: There were sixty-seven(70%)patients in the LG and twenty-nine(30%) in the NG. The median Zinc value of all patients was 73μg/dl. Median age (LG/NG) was 51/54 years old. Gender (male/female) was 33/34 in LG and 16/13 in NG. There was no difference in age, gender, extent of disease, relapse rate, CRP, or F-cal. The median pMayo score(LG/NG) was 1/0, p=0.004, and the median Mayo score(LG/NG) was 1/0, p=0.006, all significantly higher for LG. In the Zinc-supplemented patients who mainly used zinc acetate hydrate, the median values before and after Zinc supplementation were 69/101μg/dl , p=0.000, CRP 0.04/0.01 mg/dl, p=0.15, F-cal 95/63 mg/kg, p=0.22, pMayo score 1/0,p=1.0, Mayo score 2/0,p=0.31. Zinc levels were significantly improved after supplementation, but there were no differences in other parameters.
Conclusion: The serum Zinc level may be a biomarker of UC activity. Short-term observation showed no change in disease activity with Zinc supplementation, further follow-up is needed.
PP-02-046
Plasma IgA Levels Correlate with Biological Therapy and ESR Levels in Patients with Ulcerative Colitis
Jye-lin Hsu and Tsung-Yu Tsai
China Medical University, Taichung, Taiwan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Although IgA is the predominant antibody secreted by gut immune cells, the level of plasma IgA in ulcerative colitis (UC) patients undergoing biological treatment remains unknown. This study aimed to investigate the relationship between plasma IgA concentrations in UC patients receiving biological therapy.
Aim: To investigate the relationship between plasma IgA levels in UC patients undergoing biological therapy.
Methods: We enrolled 40 UC patients between 2020 and 2023. Plasma concentrations of IgG, IgM, and IgA were measured before initiating therapy, and at the 14th and 52nd weeks of biological therapy (Adalimumab, Vedolizumab, or Tofacitinib). We compared these antibody levels and their relationship with clinical manifestations. Statistical analysis was conducted using the Student's t-test.
Results: Plasma IgA levels were significantly higher before initiating biological therapy and decreased after 14 and 52 weeks of treatment in UC patients. This pattern was not observed for plasma IgG and IgM levels. Additionally, plasma IgA levels significantly correlated with ESR levels (P=0.0275, R²=0.1430). There was also a trend toward a positive correlation between plasma IgA levels and Mayo subscore, although more cases are needed to confirm this observation.
Conclusion: Baseline plasma IgA levels correlate with biological therapy in UC patients and were associated with ESR levels. IgM and IgG did not exhibit similar patterns. These findings suggest that plasma IgA levels may serve as a potential biomarker for UC patients.
PP-02-047
TNF-Alpha Inhibitors And Demyelinating Disease In Crohn’s Disease Patient : A Case Report
Karunia Valeriani Japar1, Ignatius Bima Prasetya1,2 and Rabbinu Rangga Pribadi3
1Faculty of Medicine Universitas Pelita Harapan, Kota Tangerang, Indonesia;2Fellow of Gastroenterohepatology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, CiptoMangunkusumo Hospital, Central, Indonesia;3Division of Gastroenterology Department of Internal Medicine Faculty of Medicine Universitas Indonesia, CiptoMangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The growing prevalence of Crohn's disease (CD) highlights a significant trend, though epidemiological data remains incomplete. TNF-alpha inhibitors that is used in Chron’s disease can cause demyelinating symptoms.
Case Description: We present a case of a 26-year-old female with Crohn's disease and neurological complications after being prescribed with adalimumab and infliximab, respectively. The patient initially responded well to the treatment, but over time, she began to experience a loss of strength and balance in her limbs. Brain Magnetic resonance imaging revealed a distribution pattern suggestive of demyelinating plaques, although not sufficient to diagnose multiple sclerosis. The physicians team stop all biologic agents due to suspected drug interaction causing neurological symptoms. The patient showed progressive improvement over a one-year period, eventually no longer exhibiting any demyelinating symptoms. This positive clinical outcome highlights the potential for significant neural recovery and underscores the effectiveness of the treatment protocol, which included oral methotrexate, sulfasalazine, and steroids.
Discussion: The potential for TNF-alpha inhibitors in CD to cause demyelination is concerning and often underappreciated. The exact mechanisms underlying these neurological complications are not fully understood, but one theory suggests that TNF-alpha blockers may enhance demyelination by increasing the migration of peripheral autoreactive T cells into the CNS. Previous studies estimate the incidence of demyelination due to TNF-α inhibitors to be between 0.03% and 0.2% of patients receiving these treatments.
Conclusion: Given these potential adverse effects, clinicians should exercise caution when administering TNF-alpha inhibitors.
1Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China;2Inflammatory Bowel Disease Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China;3Institute of Gastroenterology, Zhejiang University, Hangzhou, 310016, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Evidence suggests dietary advanced glycation end products (AGEs) promote oxidative stress and inflammation in the gastrointestinal tract. This study investigates the association between dietary AGE intake and the risk of inflammatory bowel disease (IBD).
Materials and Methods: A total of 123,354 participants without IBD at baseline were included from the UK Biobank. Consumption of three common AGEs (Nε-carboxy-methyllysine [CML], Nε-carboxyethyllysine [CEL], and Nδ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine [MG-H1]) was estimated by matching 24-hour dietary questionnaires to a validated dietary AGE database. Cox proportional hazards regression models calculated the hazard ratio (HR) and 95% confidence interval (CI) of associations between dietary AGEs and IBD risk.
Results: Over a median follow-up of 13.72 years, 671 participants developed IBD, including 192 with Crohn’s disease (CD) and 478 with ulcerative colitis (UC). Only CEL was associated with an increased risk of IBD (HR=1.09, 95% CI: 1.01-1.18, P=0.020) and CD (HR=1.18, 95% CI: 1.03-1.36, P=0.014), particularly in overweight, physically inactive, and non-smoking participants. For participants at high genetic risk of CD, HRs (95% CI) were 1.26 (1.00-1.57) for CML, 1.41 (1.12-1.76) for CEL, and 1.28 (1.01-1.62) for MG-H1 (all P < 0.05). None of the dietary AGEs were significantly associated with UC risk, regardless of genetic predisposition.
Discussion: Dietary CEL was associated with an increased risk of IBD and CD, but not UC, especially in those with high BMI, low physical activity, and no smoking history. Further interventional studies are needed to explore the potential benefit of AGE restriction, particularly for individuals at high genetic risk of CD.
PP-02-049
Correlation of Hematologic Inflammation Biomarker With Ulcerative Colitis Disease Activity at RSUP Wahidin Sudirohusodo Makassar
Putri Nila Kandi1, Muhammad Luthfi Parewangi2,3, Amelia Rifai2,3, Fardah Akil2,3, Nu'man AS Daud2,3, Rini Rachmawarni Bachtiar2,3 and Susanto Hendra Kusuma2,3
1Internal Medicine Specialist Program, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;2Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;3Centre of Gastroenterology-Hepatology, HAM Akil Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Ulcerative Colitis (UC) is a chronic inflammatory disease of gastrointestinal tract that use endoscopic to assess the disease activity. To determine UC, a comprehensive approach involving clinical, biochemical, histological, and endoscopic biopsy evaluations is essential. Several studies have been conducted to determine hematological markers that are easier and more affordable to perform. Erythrocyte Sedimentation Rate (ESR) and Neutrophil-Lymphocyte Ratio (NLR) have been identified as some of potential inflammatory hematological biomarkers. An elevated ESR or NLR level may suggest inflammatory bowel diseases. The aim of this study is to determine correlation between hematological inflammatory biomarkers, such as ESR and NLR with the degree of disease activity in ulcerative colitis.
Materials and Method: This research is an observational analytical study with a cross-sectional approach. With statistical test used is chi-square correlation test. A retrospective database analysis study from ulcerative colitis patients diagnosed between January-Juny 2024 in Wahidin Sudirohusodo Hospital Makassar was conducted. Information on patients demography, laboratory, and endoscopic biopsy data were collected.
Results: There are 91 subjects in total with 23 subjects was moderate to severe ulcerative colitis and 68 subjects was mild ulcerative colitis were included in this study. A chi-square test was performed and there is a significant correlation between NLR (P < 0.005) but no correlation with ESR (P > 0.005) to the UC disease activity.
Conclusion: Neutrophyl-lymphocyte ratio is one of hematological inflammation biomarker that significantly correlated with the degree of disease activity in ulcerative colitis.
PP-02-050
Effects of Zanthoxyli Pericarpium on inflammation and network pharmacological mechanism analysis in DSS-induced colitis mice
Woo-Gyun Choi1, Daehwa Jung2, Sang Chan Kim3, Na Ri Choi1,4 and Byungjoo Kim1
1Department of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, South Korea;2Department of Pharmaceutical Engineering, Daegu Hanny University, Gyeongsan, South Korea;3College of Oriental Medicine Daegu Haany University, Gyeongsan, South Korea;4Department of Korean Medical Science, Pusan National University School of Korean Medicine, Yangsan, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Inflammatory bowel disease (IBD) is a condition characterized by chronic inflammation of the intestines. Zanthoxyli Pericarpium (ZP) has long been utilized in traditional oriental medicine for managing intestinal disorders due to its known anti-inflammatory and antioxidant properties. The researchers aimed to investigate the components of ZP and their specific targets, as well as associated diseases. Additionally, we sought to explore the protective effects of ZP on the colon and uncover the underlying molecular mechanisms in treating IBD.
Materials and methods: The study employed the TCMSP analysis platform and database to identify the compounds in ZP and their interactions with specific target genes related to IBD. Furthermore, the researchers conducted experiments using a murine model of colitis induced by dextran sodium sulfate (DSS) to assess the impacts of ZP.
Results: The network pharmacological analysis revealed that 17 compounds in ZP target 20 genes associated with IBD, including AKT1, CASP3, CCL2, CLDN4, CRP, CXCL10, CXCL2, HMOX1, ICAM1, IFNG, IL10, IL1B, IL2, IL6, MPO, NFKBIA, PPARG, PTGS2, STAT1, and TNF. In the DSS-induced colitis model, ZP demonstrated a significant reduction in colonic epithelial damage caused by DSS. Moreover, ZP effectively reduced the levels of oxidative stress markers, such as iNOS and nitrotyrosine, indicating its antioxidant properties.
Conclusions: The study provides evidence supporting the protective effects of Zanthoxyli Pericarpium (ZP) against DSS-induced colonic damage, primarily attributed to its anti-inflammatory and antioxidant properties. These findings suggest that ZP could be a potential candidate for further exploration as a treatment for inflammatory bowel disease (IBD).
PP-02-051
Correlation of Disease Activity, Fatigue, and Quality of Life in Patients with Ulcerative Colitis
Seung-Jun Kim
CHA Bundang Medical Center, Bundang-gu, Seongnam-si, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The aim of this study was to investigate the correlation of disease activity with fatigue and quality of life (QoL), as well as the IBDQ score as a surrogate disease monitoring marker.
Method: A total of 30 patients with UC were enrolled in this study. The patients were asked to fill out questionnaires on the day they received colonoscopy. Disease activity was assessed by the Mayo score (MS), fatigue levels were measured by multidimensional fatigue inventory Korean version (MFI-K) questionnaire, and QoL was measured by inflammatory bowel disease questionnaire (IBDQ).
Results: Patients with moderate to severe disease activity displayed significantly higher levels of general and physical fatigue (16.2 vs 18.1, p=0.001) and reduced activity (14.6 vs 13.0 p=0.034), while mental fatigue (18.6 vs. 18.3, p=0.781) and motivation (9.2 vs. 10.6, p=0.250) were not significantly correlated with disease activity. Furthermore, patients with moderate to severe disease activity had significantly lower IBDQ scores compared to those with remission to mild disease activity (116.0 vs. 190.4, p<0.001). Using the ROC curve, we established that when the IBDQ score was cut off at 173, the sensitivity for indicating moderate to severe disease activity was 89.5%, and the specificity was 90.9%.
Conclusion: Impaired QoL and increased fatigue, especially in categories of general and physical fatigue and reduced activity, are significantly correlated with higher disease activity. And it is recommended to perform an endoscopic workup when the IBDQ score is above 173.
PP-02-052
Eight cases of ulcerative colitis with mesalazine intolerance
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Mesalazine is the first-line drug used for the initial treatment of ulcerative colitis (UC). Since only a few cases of mesalazine intolerance have been reported, we reviewed such eight cases.
Materials and Methods: We investigated the clinical background and treatment course of eight patients with mesalazine-intolerant UC treated in our hospital from January 2016 to December 2023. The median age of the patients was 50.5 (16-77) years, and the male to female ratio was 6:2. All patients had new-onset UC and the types were: pancolitis (5), right-sided colitis (1), left-sided colitis (1), and proctitis (1). Six patients received multi-matrix system-mesalazine, one received pH-dependent mesalazine, and one received time-dependent mesalazine as first-line treatments.
Results: The median number of days from the start of treatment to symptom exacerbation was 10 (8-360). The drug lymphocyte stimulation test was positive in five patients and negative in three. Remission was achieved in all patients with mesalazine discontinuation and remission-induction therapy: systemic glucocorticoid administration and granulocytapheresis, systemic glucocorticoids, steroid enema, Ustekinumab (steroid was ineffective), and mesalazine discontinuation only were 1, 1, 3, 1, 2, respectively. Remission maintenance therapy used azathioprine, ustekinumab, golimumab, steroid enema, and no medication in three, one, one, one, and two patients, respectively.
Conclusion: Drug intolerance was considered on sudden worsening of UC symptoms approximately 10 days after the initial mesalazine treatment. Although intolerance rarely develops after a prolonged course of treatment, it is necessary to be cognizant of this possibility early in the use of mesalazine.
PP-02-053
Deep learning model using stool pictures discriminates UC patients from subjects with normal colonoscopy
O Seong Kweon and Eun Soo Kim and Dong Won Woo and Kyeong Ok Kim and Sung Kook Kim and Hyun Seok Lee and Ben Kang and Yoo Jin Lee and Byung Ik Jang and Eun Young Kim and Hyeong Ho Jo and Yun Jin Chung and Han Jun Ryu and Soo Kyung Park and Ho Sang Yu and Sung Moon Jeong
Kyungpook National University School of medicine, Daegu, South Korea;Kyungpook National University Hospital, Daegu, South Korea;Yeungnam University College of Medicine, Daegu, South Korea;Keimyung University School of Medicine, Daegu, South Korea;Daegu Catholic University School of Medicine, Daegu, South Korea;Daegu Fatima Hospital, South Korea;Sungkyunkwan University School of Medicine, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Diagnosis of ulcerative colitis (UC) is challenging as there is no gold standard diagnostic tool of the disease.
We previously developed deep learning model using stool photos for predicting endoscopic activity in 306 patients with UC (DLSUC)
This study aimed to explore the potential of the DLSUC in the diagnosis of UC.
Materials and Methods: Patients with endoscopically active UC and subjects for colonoscopy with various symptoms were prospectively enrolled in the study.
They were asked to take stool pictures with their smartphones 1 week before undergoing endoscopy.
Area under the receiver operating characteristic (AUC), sensitivity, specificity, and accuracy to discriminate UC patients from subjects without UC were estimated.
Results: The deep learning model for screening ulcerative colitis patients, demonstrated an accuracy of 0.8718, a sensitivity of 0.80636, and a specificity of 0.8824, outperforming fecal calprotectin, which showed an accuracy of 0.7941, a sensitivity of 0.8095, and a specificity of 0.7692
In validation set, area under curve (AUC) for predicting endoscopic activity using AI model was 0.914 which was higher, but not statistically different from AUC using Fcal (0.82, in previous study)
Conclusion: Stool photos of UC patients with deep learning technology demonstrated similar accuracy to Fcal in discriminating active UC patients from subjects with normal colonoscopy.
Deep learning model showed sufficient accuracy differentiating IBS patients with UC patients.
Artificial intelligence and smartphone technology using stool photos showed potentials as one of the adjunctive tools for assessing mucosal inflammation of UC patients and screening of suspected UC patients.
PP-02-054
Multi-omics phenotyping characterizes perturbed molecular divergence underlying different clinical scenarios of under-treatment inflammatory bowel disease
Hongsub Lee
Inje University College of Medicine, Busan, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Clinically heterogeneous spectrum and molecular phenotypes of inflammatory bowel disease (IBD) remain to be comprehensively elucidated. The serum proteome, metabolome, and lipidome of 75 IBD patients were profiled. Compared to Crohn’s disease, ulcerative colitis showed a lower abundance of hypoxanthine correlating negatively with cell adhesion-related proteins of ITGB2, CD200, PRELP, and positively with lysophosphatidylcholines [LPC(16:0-SN1), LPC(O-18:1)]; and up-regulation of bile acids and FGF19. For (II), while proteins related to inflammatory-promoting signaling pathways, sphingomyelins (SMs), and LPC were increased; bile acids, amino acids, and triacylglycerols (TGs) were decreased. Not only in (II), TGs were a major class change in (III), whose abundance was consistently higher in the active state. This, along with decreased SMs levels, correlated strongly with the up-regulation of inflammatory-inducing proteins (CXCL9, CXCL1, ITGB2, IL17A). In (IV), treatment escalation promoted augmentations of signaling pathways-related proteins and triggered significant metabolome responses, especially decreased amino acids. Noticeably, the levels of TGs were consistently increased in the post-treatment escalation group, thereby suggesting treatment response with relation to (III). For (V), down-regulation of signaling pathways-related proteins and higher levels of SMs and PCs was observed. A consistent decrease of TGs in patients who needed treatment escalation along with lower level of TGs in elevated calprotectin group could imply for the inflammatory level. This study provided the multi-ome profiles of IBD patients in five clinical scenarios and laid a foundation for opening new doors for disease management and treatment.
PP-02-055
Prevalence of Inflammatory Bowel Disease in Kinta Valley, Malaysia: An Update after a Decade
Hak Keith Leung1, Xin-Hui Khoo1, Yi Wern Khong1, Suh Yu Cheong2, Phei Onn Tan2, Andrew Seng Boon Chua3, Andrew Seng Boon Chua4, Harjinder Singh5, Thean Heng Ng5, Choon Heng Wong6, Wai Hong Kong6 and Ida Normiha Hilmi1
1University Of Malaya, Kuala Lumpur, Malaysia;2Hospital Raja Permaisuri Bainun, Ipoh, Malaysia;3Ipoh Gastro Centre, Ipoh, Malaysia;4Hospital Fatimah, Ipoh, Malaysia;5Pantai Hospital Ipoh, Ipoh, Malaysia;6KPJ Specialist Centre, Ipoh, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This study aimed to update Malaysia's current prevalence of inflammatory bowel disease.
Methods: All IBD cases in five hospitals in Kinta Valley were recruited retrospectively, and attempt was made to contact all resident cases who lost follow-up. Uncontactable cases were put in request to National Registry Department to inquire on death registration. The demographics and clinical characteristic were obtained from medical records.
Results: 153 case notes of IBD patients in Kinta valley were reviewed. Six patients had deceased. Two patients were uncontactable. 145 confirmed resident cases of IBD was identified. Among them, there were 56.6% males, 43.4% females; 29.0% Malays, 32.4% Chinese, 38.6% Indians. Median age of diagnosis was 38.0(25.0-52.0). The distribution of the cases was as follows: 102 UC(70.4%), 42 CD(28.9%) ,1 IBD-U(0.7 %). Among CD patients, there were 58.1% non-stricturing non-penetrating cases, 35.5% stricturing cases, 9.6% penetrating, and 25.0% perianal disease. The disease location was 29.0% terminal ileum, 35.5% colonic, 35.5% ileocolonic, 12.9% isolated upper disease. Among UC patients, 32.2% were proctitis, 35.6% were left-sided, 32.2% were extensive. The prevalence rates were 16.31, 11.48, 4.73, 0.11 for IBD, CD, UC and IBD-U per 100,000 persons respectively. The highest prevalence rate was among Indians(50.77 per 100,000 person), followed by Chinese(14.61 per 100,000 person) and Malays(10.43 per 100,000 persons).
Conclusions: There was an increased prevalence of IBD cases in Kinta valley compared to a decade ago. There is also reversal of prevalence between Chinese and Malay population, whereby there were more Chinese IBD patients diagnosed within the decade.
PP-02-057
Luminal Zinc Induces Clostridioides difficile Virulence and Exacerbates Gut Inflammation in UC patients
Jie Liang and Tong Wu
Xijing Hospital, Xi'an, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: The disease severity of Ulcerative Colitis (UC) is often complicated by C. difficile infection (CDI). However, the actual role of C. difficile in UC development remains unknown.
Methods: UC patients’ stool samples (n=60) were collected and analyzed by qPCR and ICP-MS to determine the presence of C. difficile and the fecal zinc concentrations. C. difficile was cultured in BHIs with or without supplemented zinc in an anaerobic chamber. Expression of virulence genes was analyzed and biofilm assays were used to examine C. difficile flagellar formation. Human colonic organoids and Caco-2 cells were co-cultured with C. difficile or zinc-treated C. difficile, and cytotoxicity, permeability, and inflammatory cytokine release were assessed. TLR5 knock-out Caco-2 cells were constructed and co-cultured with C. difficile or zinc-treated C. difficile to verify whether zinc-induced C. difficile’s pathogenicity was mainly through flagellar formation.
Results: C. difficile was detected in ~35% of UC patients’ fecal samples. Luminal zinc concentrations (~915μM, p<0.01) were significantly higher among UC patients than CD patients or healthy subjects. The UC patients’ luminal zinc concentration promoted C. difficile growth, suppressed its exotoxins release, and induced higher flagella-related gene expression and flagellar formation, resulting in higher cytotoxicity and inflammatory responses in human colonic organoids and Caco-2 cells. Also, zinc-treated C. difficile did not induce higher inflammation and pathogenicity in TLR5 knock-out Caco-2 cells.
Conclusion: The association between C. difficile and UC aggravation may partially stem from UC patients’ excess luminal zinc, which promotes C. difficile flagellar formation, induces inflammatory responses, and worsens UC progression.
PP-02-058
SLC7A5 promotes the progression of UC by regulating amino acid transport and intestinal epithelial autophagy
Jie Liang and Yanting Shi
Xijing Hospital, Xi'an, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Inhibition amino acid metabolism of inflammatory sites may be effective in alleviating UC (ulcerative colitis). SLC7A5 is involved in the progression of UC, but the mechanism of its functional regulation still needs to be investigated.
Methods: We previously discovered through high-throughput screening that SLC7A5, as a cellular amino acid transport carrier, is significantly elevated in the colonic tissues of UC patients. This study utilized model animals, transcriptomics, amino acid-targeted metabolomics assays, and macro-genome sequencing to elucidate the mechanism of SLC7A5-regulated amino acids in mTOR pathway activation and intestinal autophagy dysfunction.
Results: SLC7A5 expression is increased in the colon of UC patients and DSS-induced mice lesions. SLC7A5 inhibitor (JPH203) restrained the inflammatory responses induced by DSS. SLC7A5 deletion or inhibition dampens the release of IL-1β, IL-18, and IL-23 and the production of ROS in the LPS-induced FHC and RAW264.7 cells. Moreover, upregulating SLC7A5 expression induces mTOR signaling pathway activation in FHC cells. Deletion or inhibition of SLC7A5 efficiently blocks the SLC7A5-dependent amino acid transport, inhibits the mTOR activation, and results in the activation of autophagy.
Conclusion: Targeting SLC7A5-mediated amino acid uptake is a potentially useful immunosuppressive strategy to regulate colonic inflammation through mTOR pathway and autophagy. This study is expected to reveal the intrinsic factors of metabolic disorders promoting the UC progression from the perspective of amino acid metabolism, and to lay a new theoretical and experimental foundation for potential UC treatment.
Department Of Gastroenterology And Hepatology, General Hospital, Tianjin Medical University, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The surge in inflammatory bowel disease (IBD) is closely related to the dysbiosis of the intestinal microbiota and the disruption of the intestinal barrier. We found that Desulfovibrio vulgaris fagellin (DVF) enhanced histone H3K27 methylation (H3K27me3), decreased the expression of tight junction protein ZO-1 and disrupted the intestinal barrier, and H3K27 methylase screening showed that the expression of PRC2 core subunit-EED was increased. Therefore, we hypothesized that DVF inhibits ZO-1 expression by promoting EED-mediated H3K27 methylation, thereby disrupting the tight junction of the intestinal epithelium.
Materials and Methods: The correlation between Desulfovibrio vulgaris (DSV) abundance, EED expression and intestinal inflammatory activity was investigated in IBD patients. Cell transfection, EED inhibitors and CUT&Tag experiments were used to explore the mechanism of DVF regulating the EED-H3K27me3-ZO-1 axis.
Results: Disease activity in UC patients was positively correlated with DSV abundance and EED expression. After DVF stimulation of Caco2 cells, the expression of ZO-1 protein decreased and the expression of inflammatory factors increased. EED expression was knocked down in Caco2 cells, and ZO-1 and inflammatory factor expressions were unchanged. CUT&Tag sequencing of stimulated Caco2 cells confirmed that the expression of H3K27me3 was increased and ZO-1 expression was inhibited. After DVF stimulation in mice, the expression of intestinal inflammation increased, the expression of H3K27me3 and EED increased, and the expression of ZO-1 decreased. This change can be eliminated with the inhibitor of EED226.
Conclusion: DVF promotes H3K27 histone methylation modification in intestinal epithelial cells and destroys the intestinal barrier to induce IBD.
PP-02-060
Comparative efficacy and safety of therapies for adult ulcerative colitis: a network meta-analysis
Zilin Ma
17735972555, Zikai Wang
Xiaoyan Chi
13467853456, Fengqi Liu, Rongrong Ren
PLA General Hospital, Beijing, China
Objectives: To evaluate the efficacy and safety of mesalazine, glucocorticoids, immunosuppressants, small-molecules, biologics, and fecal microbiota transplantation in treating UC in adults.
Materials and Methods: Literature search in Cochrane, Embase, and PubMed databases up to June 2024 identified RCTs involving adult UC patients treated with the aforementioned therapies. Clinical remission and mucosal healing were the primary efficacy outcomes, with adverse events (AEs) and serious AEs as the safety outcomes. A network meta-analysis was conducted using a multivariate consistency model and random-effect meta-regression, with treatment rankings based on the surface under the cumulative ranking curve (SUCRA). Cluster analysis was applied to assess the SUCRA scores for efficacy and safety, identifying the optimal treatment.
Results: 71 RCTs, 10,338 UC patients included. Tacrolimus showed the highest clinical response rate (RR 0.25, 95% CI 0.06-0.57; SUCRA, 93.7%) and mucosal healing. Mercaptopurine (6-MP) had the highest rank for clinical remission (RR=0.63, 95%CI 0.55-0.86; SUCRA, 85.8%) but the lowest for AEs (RR=1.25, 95%CI 1.20-1.80; SUCRA, 14.2%). Only Vedolizumab reduced AEs, with the highest adverse event ranking (RR=0.38, 95%CI 0.25-0.79; SUCRA, 95.1%). Infliximab uniquely increased the risk of serious AEs (RR=1.70, 95%CI 1.52-1.96). Cluster analysis of clinical response and AEs indicated Vedolizumab as the most efficacious treatment for UC (Figure 1). The study's conclusions should be cautiously interpreted due to potential biases and heterogeneity.
PP-02-061
Comparing TRIple Rectal Ultrasound Imaging Technology in Ulcerative Colitis(TRINITY Study):Trans abdominal, perineal and Endoscopic ultrasound
Partha Pal, Mohammad Abdul Mateen, Anuradha Sekaran, Kanapuram Pooja, Jahangeer Basha, Rajesh Gupta, Manu Tandan, Sundeep Lakhtakia and D Nageshwar Reddy
Asian Institute Of Gastroenterology, Hyderabad, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To compare the accuracy of transperineal ultrasound (TPUS) with endoscopic ultrasound (EUS), endoscopic activity, and histologic activity in assessing rectal inflammation in ulcerative colitis (UC).
Methods: Consenting UC patients (18-75 years) underwent trans-abdominal ultrasound (TAS), TPUS, sigmoidoscopy (with biopsy), and EUS in the same session to correlate rectal total wall thickness (TWT) and vascularity across TAS, TPUS, and EUS with endoscopic and histologic activity.
Results: 49 UC (median age 38 years, 57.1% male) were included. The area under the curve (AUC) to predict endoscopic activity was highest for EUS [mucosal thickness>0.95mm, sensitivity(Sn): 95%, specificity(Sp): 85%, AUC: 0.93; TWT >3.9 mm: AUC: 0.79), vascularity (Modified Limberg score-MLS)> 1: AUC: 0.85, submucosal thickness (SMT)>2.1mm: AUC: 0.77) followed by TPUS (TWT>5.7 mm: AUC: 0.619, MLS>1: AUC: 0.62) and TAS (TWT >6.4 mm: AUC: 0.50; MLS>1: AUC: 0.5) (Figure 1A; p values, 95% CI, 1B-C: Receiver operating characteristic curves).
Prediction of histologic activity (Nancy Index) (n=26) was best with EUS (MLS≥ 1; Sn: 90%, Sp: 80%; AUC: 0.91; SMT >2.1mm: AUC: 0.75; MT >1mm: AUC: 0.72; TWT >3.9 mm: AUC: 0.64) followed by TPUS (TWT >5.6 mm: AUC: 0.77; MLS ≥1: AUC: 0.67) and TAS (TWT >6.4 mm: AUC: 0.54; MLS ≥1: AUC: 0.57) (Figure 1D,p value,95%CI, E-F: ROC curve).
Conclusion: TPUS shows promise in assessing rectal inflammation in UC but is less accurate than EUS for predicting endoscopic and histologic remission. EUS remains the gold standard for evaluating rectal inflammation, while TPUS can serve as a complementary, non-invasive tool with moderate accuracy.
PP-02-063
Flow of Advanced Therapy Pathways and Variations over Time in Crohn’s Disease and Ulcerative Colitis
Fei Yang Pan1,4, Aviv Pudipeddi2,3, John Chetwood2,3 and Rupert Leong2,3
1Macquarie University Hospital, Sydney, Australia;2University of Sydney, Concord, Australia;3Concord Repatriation General Hospital, Concord, Australia;4Macquarie University, Sydney, Australia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The choice of advanced therapy (AT) in Crohn's disease (CD) and ulcerative colitis (UC) has increased, but the optimal sequencing of agents is unknown. We present the first national data on AT treatment sequencing in Australia using Sankey diagrams.
Methods: We analysed the Persistence Australian National IBD Cohort (PANIC) 5 registry, including UC and CD patients on AT via the Pharmaceutical Benefits Scheme up to December 2021. Sankey diagrams were created using Plotly. Statistical analyses were performed using SPSS.
Results: Among 9,671 UC patients (23,220 patient-years), VED patients were more likely to stay on treatment compared to TNFi (P<0.001). TOF was less likely to be used in bio-naïve patients and more commonly in bio-exposed patients. TOF increased from 85 to 397 patients (366%). UC patients were 13.1 times (P<0.001) more likely to stop treatment in T1 compared to T3.
Among 19,087 CD patients (79,677 patient-years), VED patients were more likely to stay on treatment compared to TNFi (P<0.001). UST was 2.5 times more likely to be used in bio-exposed patients compared to ADA. CD patients were 13.6 times (P<0.001) more likely to stop treatment in T1 compared to T3. UST increased from 2,231 to 4,294 patients (92%), and VED from 1,171 to 1,690 patients (44%).
Conclusion: This study highlights diverse AT pathways for IBD patients, leading to greater treatment retention over time, especially for TOF and UST compared to IFX, ADA, VED, and GOL. There is an increasing trend of TOF and UST use as salvage therapy.
PP-02-064
Intestinal Behçet’s disease: clinical features and management In a Korean tertiary hospital
Subum Park, Cheolmin Lee and Cheolwoong Choi
Department of Internal Medicine, Pusan National University School of Medicine and Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Behçet’s disease(BD) is an inflammatory vasculitis disease characterized by recurrent oral and genital ulcers, ocular inflammation, and skin lesions. Behçet’s patients can also present with gastrointestinal lesions. Although gastrointestinal symptoms in BD are noted in half, intestinal-BD is rare. We evaluated the clinical features and treatment of patients with intestinal-BD at a single center in Korea.
Methods: 327 patients who met the international study group criteria for BD, followed up in a tertiary center from November 2008 to September 2023 were evaluated in this medical records review study. Among 327 BD patients, 261 were BD without GI involvement, and 66 intestinal-BD patients were enrolled. Data of demographics, clinical manifestations, laboratory and treatment were assessed and compared between two groups.
Results: The mean age of 327 BD patients was 43.42years.
In laboratory findings, HLA-B51 demonstrated a significant difference.
In clinical manifestations, uveitis, skin lesions, arthritis, abdominal pain and GI bleeding showed statistical significance.
In treatment, 5-ASA, azathioprine and adalimumab demonstrated a significant difference.
Intestinal BD showed higher in operations and hospitalization compared to BD.
Conclusions: This cohort of Korean patients with intestinal BD showed a predominance of females, although not statistically significant. Laboratory findings indicated a lower prevalence of HLA-B51 among intestinal BD patients, and clinical manifestations revealed a lower incidence of uveitis, skin lesions, and arthritis. In terms of treatment, we observed a higher frequency of 5-ASA, AZA, and adalimumab in intestinal BD, and noted a higher rate of surgery and hospitalization in patients with intestinal BD.
PP-02-065
Inflammatory Bowel Disease Patients need Higher Opioid Analgesic for Intravenous Conscious Sedation for Colonoscopy.
Young Sook Park, Gyeol Song, Sang Bong Ahn and Young Mi Jeon
Eulji University/ Nowon Eulji University Hospital, Seoul, Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Patients with inflammatory bowel disease (IBD) require colonoscopy for diagnosis, disease activity assessment, and dysplasia surveillance. Patients with IBD reported lower satisfaction with sedation during colonoscopy and increased procedural pain compared with individuals without IBD. This study aimed to examine sedation requirements of IBD patients undergoing colonoscopy.
Materials and Methods: A retrospective cohort study of IBD and non-IBD patients presenting for colonoscopy between January 2017 and December 2021 was undertaken. Data collected included patient and procedure focused variables. Sedation was performed as intravenous conscious sedation (IVCS) using midazolam and propofol. The opioid analgesic, pethidine was added If the sedation level was not adequate for procedures.
Results: A total of 1655 consecutive colonoscopies (253 IBD, 1402 non-IBD) were analyzed. For IVCS, 11.1% of IBD patients required Midazolam only(M), 41.9%, Midazolam and propofol (MP), and 47.0%, Midazolam, Propofol and opioid (MPO) versus 11.7% of non-IBD required M, 69.1% MP and 19.2% MPO respectively. Among IBD patients (CD 52, UC 201), active disease state required MPO in 51,6% (66/128) versus inactive state 42,4% (53/125) (p=0.18). Propofol administration (mg) was 25.4±13.3 in IBD and 23.7±12.7 in non-IBD (p=0.07).
Conclusion: IBD patients required more opioid analgesic for IVCS compared with non-IBD patients. IBD patients showed similar opioid requirement both active and inactive disease state.
PP-02-066
Assessment of anemia in patients with inflammatory bowel disease and its correlation with worse outcomes
Viviana Parra-izquierdo and Ginary Orduz and Juan Frias and Silvia Hoyos and Juliep Sarmiento and Oscar Pinto and Alexis Duarte and Carlos Cuadros and Johon Garces and Andres Barco and Javier Acevedo and Javier Fonseca and Cristian Florez and Javier Fonseca
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: To describe the presence and type of anemia in patients with Inflammatory Bowel Disease (IBD) and its relation with relapses, related complications and disease and its relationship with relapses of the disease, complications related to this and the need to start need for initiation of biologic therapy.
Materials and Methods: Cross-sectional, descriptive observational study of patients with patients with Inflammatory Bowel Disease who have been admitted to the Center of Excellence and have presented with anemia during the last 3 months of their disease, establishing its relationship with relapses, complications and the use of biologic therapy.
Results: 46 patients evaluated, 40% of whom had anemia, of whom 59% were patients with ulcerative colitis and 41% with Crohn's disease. 59% were patients with ulcerative colitis and 41% patients with Crohn's disease. For the group of patients with ulcerative colitis group of patients with ulcerative colitis, it was found that 80% had anemia of chronic disease characteristics, 10% anemia due to chronic disease, and 10% anemia due to Crohn's disease. disease, 10% with anemia due to vitamin B12 deficiency and 10% with anemia in the context of mileodysplastic syndromes.
Conclusions: Early diagnosis of anemia in patients with inflammatory bowel disease is essential.
Inflammatory Bowel Disease, it was demonstrated in our group of patients its relation with the extent and severity of the of the disease, as well as the increase in the number of relapses and the requirement of biologic therapy, including combination therapy.
PP-02-067
Translation and implementation IBD-F fatigue scale in a Colombian center for IBD: an observational study.
Viviana Parra-izquierdo and Juan Frias and Ginary Orduz and Silvia Hoyos and Andrea Reatiga and Juliep Sarmiento and Luis Bohorquez and Oscar Pinto and Hernan Duarte and Carlos Cuadros and Johon Garces and Juan Acevedo and Andres Barco and Manuel Ardila and Cristian Florez
Leuven University, Bucaramanga, Colombia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Fatigue is a frequent symptom associated with inflammatory bowel disease (IBD). In Colombia, there is no history of fatigue assessment in patients with IBD, and an initial approach is required. The objective of this study was to evaluate IBD patients with IBD in a reference center for IBD in Colombia with the IBD fatigue scale (IBD-F).
Materials and methods: Descriptive observational cross-sectional study in patients with IBD in a Colombian reference center, during the year 2023, using the IBD-F scale.
Results: Thirty-two subjects were included, predominantly men (53.1%), mean age 40.1 years (SD 15.6; range 17-81), all in disease remission, and history of disease activity in the last 2 years, 62.5% with ulcerative colitis. At the time of the survey, in 53.1% the level of fatigue was zero. When evaluating average fatigue in the last 2 weeks, in 59.5% gave a score >1. In terms of fatigue impact, 46.8% had to take a nap during the day because of fatigue, and in 37.4% fatigue affected the ability to do normal household activities, and 37.5% had their quality of life affected by fatigue. Disease activity was the main cause related to fatigue.
Conclusions: Assessment of fatigue by IBD-F is comprehensive, disease-focused, and useful in clinical practice, and should be part of comprehensive management in IBD.
PP-02-068
Digital-chromoendoscopy is associated with increased detection of colorectal cancer in Latin-American patients with ulcerative colitis
Viviana Parra-izquierdo and Juan Frias and Edgar Ibañez and Fabian Juliao and Fabio Gil and Hernando Marulanda and Lina Otero and Elder Otero and Fabian Puentes and Lazaro Arango and Gerardo Guzman and Kenneth Suarez and Keyla Villa and Juan Paredes and Maria Jara and David Andrade and Cristian Florez and Abel Sachez and William Otero-Regino
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The prevalence of colorectal cancer in patients with ulcerative colitis is higher than in the general population, in Latin America there is progressive increase of Ulcerative Colitis and information on screening for colorectal cancer in inflammatory bowel disease in our region is scarce.
Objective: The main objective of this study is to analyze the findings of endoscopic surveillance of colorectal cancer in patients with ulcerative colitis in Latin America according to the available endoscopic technology.
Materials and Methods: Cross-sectional, multicenter, analytical study, performed in Latin American countries, in patients with Ulcerative Colitis with more than 8 years of diagnosis or with association with primary sclerosing cholangitis. Surveillance colonoscopies were performed according to available technology. Risk factors for dysplasia detection were analyzed.
Results: 144 patients, 55.5% women, mean age 47.3 (range 17.1-90; SD 15.64) years and mean time of disease 12.71 (range 0.64-57.13; SD 8.08) years, in whom 49 lesions were identified, 18 corresponded to dysplasia. The detection rate of dysplasia per lesion and per procedure was 36.7% and 12.5%, respectively. By logistic regression analysis, time of disease OR 1.12; 95%CI: 1.047-1.215, p=0.002) and the presence of pseudopolyps (OR 3.4; 95%CI: 1.11-10.36, p=0.031) were the risk factors for higher detection of dysplasia. Digital chromoendoscopy was associated with greater detection of dysplasia (OR 4.99, 95%CI 1.092-22.864, p=0.038) than white light.
Conclusions: This is the first Latin American study evaluating real-world experience in surveillance of dysplasia and colorectal neoplasia in patients with ulcerative colitis.
PP-02-069
Breaking barriers in the pediatric patient witi ibd transition process
Viviana Parra-izquierdo and Ginary Orduz and Juan Frias and Carlos Cuadros and Andres Reatiga and Juliep Sarmiento and Luis Bohorquez and Diego Cano and Oscar Pinto and Hernand Duarte and Juan Aceved and John Garces and Andres Barco and Cristian Florez
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The diagnosis of Inflammatory Bowel Disease (IBD) includes any stage of life, and being a chronic disease, it is necessary to be accompanied by a multidisciplinary group of specialists, as well of specialists, as well as the family nucleus and an adequate social environment.
Materials and Methods: A descriptive cross-sectional study describing the implementation of the pediatric implementation of the pediatric transition protocol in patients of the Center of excellence in Inflammatory Bowel Disease.
Results: 10 patients, with a minimum age of 7 years and a maximum age of 17 years, of which 50% were diagnosed with ulcerative colitis and 50% with Crohn's disease. 70% were female. Regarding treatment, 60% required biologic therapy, with a higher percentage for the use of Infliximab, followed by Adalimumab with 33.3% and 2 cases of combined Ustekinumab + Ustekinumab + Adalimumab with 33.3%, and another case of Vedolizumab + Upadacitinib. In 30% with requirement of corticosteroids. In terms of the need for hospitalization, 70% of the pediatric population of the Center of Excellence required hospitalization due to disease outbreak. 20%, due to age, were admitted to the pediatric transition protocol , and social and family barriers were identified in 100% of them. For which a strict follow-up was carried out with the nurse, gastroenterologist and the nurse, the adult and pediatric gastroenterologist.
Conclusions: there is a need for a pediatric transition protocol that establishes the importance of taking into account the main changes in the pediatric patient.
PP-02-070
Experience of accelerated induction with infliximab in a center of inflammatory bowel disease in colombia
Viviana Parra-izquierdo and Sergio Romero and Carlos Cuadros and Juan Frias and Johon Garces and Silvia Hoyos and Ginary Orduz
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Infliximab treatment has proven be effective in inducing and maintaining remission in patients with high-risk or high-risk patients with high-risk or severe inflammatory bowel disease, which is why accelerated induction therapy at higher doses and at shorter intervals has been and shorter intervals has been proposed as a strategy to achieve a more rapid and complete therapeutic response in patients with active disease.
Objective: To describe the clinical and paraclinical characteristics and outcomes of 5 patients with inflammatory bowel disease who received accelerated induction therapy with Infliximab.
Materials and Methods: Retrospective cross-sectional descriptive study in which the clinical, and paraclinical characteristics and outcomes of 5 patients with inflammatory bowel disease who were patients with inflammatory bowel disease.
Results: 5 patients with severe acute inflammatory bowel disease, refractory to other therapies other therapies, who received accelerated induction therapy with varying doses and intervals of infliximab, 3 with Crohn's disease and 2 with ulcerative colitis, of which 20% (1 patient with Crohn's disease) achieved clinical remission, 80% (1 patient with Crohn's disease) achieved achieved clinical remission, 80% (4 patients) did not achieve induction of remission, 60% (3 patients) required alternative therapies for symptom control but only 20% achieved remission following the addition of small molecule therapy.
Conclusions: it is evident that the results are not satisfactory, this treatment strategy is not effective, however, it is still an option in acute, very severe and refractory cases of the disease.
PP-02-071
Real-world experience with intestinal ultrasound use in colombian patients with ibd: an observational study
Viviana Parra-izquierdo and Samuel Cubillos-rodriguez and Melquisedec Vargas and Edgar Ibañez and Silvia Rios and Ginary Orduz and Silvia Hoyos and Cristian Florez
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Intestinal ultrasound is a new tool in assesing IBD activity. Our objective is to describe the ultrasonographic findings in patients with Ulcerative Colitis and Crohn's Disease, as well as to evaluate the Milan Ultrasound Criteria to evaluate the Milan Ultrasound Criteria (MUC) as a predictor of activity and its correlation with disease activity in disease activity in Colombian patients with IBD
Materials and Methods: Comparative study, in patients with Inflammatory Bowel Disease who were evaluated with Intestinal Ultrasound.
Results: 46 subjects with inflammatory bowel disease, 40 with ulcerative colitis and 6 with Crohn's disease, both adults and pediatric, predominantly male, were included.
In subjects with ulcerative colitis, the colonic wall thickening (CWT) median (CWT) in patients in endoscopic remission (Mayo score 0-1) were 2.52 (RIQ 2.1 - 2.82) mm compared to 3.5 (RIQ 2.1 - 2.82) mm in patients in endoscopic remission (Mayo score 0-1). 2.82) mm compared to 3.12 (RIQ 2.18-4.2) mm for patients with endoscopically active disease (Mayo 2). In subjects with Crohn's disease, in 4/6 there was evidence of a increase of more than 3mm in the wall thickness, as for the Limberg scale, 4 patients in grade 1, 1 in grade 2 and 1 in grade 3.
Conclusions: Intestinal Ultrasound in patients with Ulcerative Colitis and Crohn's Disease is a useful and effective tool in the evaluation of disease activity in Ulcerative Colitis and Crohn's Disease.
PP-02-072
Characterization of patients with IBD and extraintestinal manifestations from Colombia: an observational study
Viviana Parra-izquierdo and Juan Frias and Juan Marquez and Fabian Puentes and Fernando Sarmiento and Rafael Garcia and Melquisedec Vargas and Gustavo Reyes and Manuel Ardila and Carolina Samper and Cristian Florez
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Information about extraintestinal manifestations (EIM) in inflammatory bowel disease (IBD) is scarce in Latin America and its implications are unknown. The aim of this study was to describe demographic characteristics, clinical presentation, phenotype and treatment of patients with IBD and EIBM in our setting.
Materials and methods: Multicenter descriptive observational multicenter study in adult and pediatric patients with IBD and IMD, in which information was collected on different dates in different institutions of patients diagnosed with IBD, treated as outpatients in different Colombian cities in 2021.
Results: out of a total of 266 patients, 197 (74.1%) presented MEI, predominantly women (67.5%). 95% were adults, and 5% were pediatric patients. 134/197 (68%) with ulcerative colitis (UC), and (63/197) 32% with Crohn's disease (CD), mostly in remission (82.2%) and requiring biologic therapy in 49.2%. Regarding the type of MEI, joint MEI were the most frequent (36%), followed by skin (34.5%), and ocular (24.9%). The frequency of hospitalization due to disease activity was 72.5%, overall vaccination rate was over 70%, and the frequency of cancer was 1.5%. There was a low rate of complications and hospitalization for COVID-19. In women, pregnancy complications were more frequent in CD.
Conclusions: The MEI in IBD are frequent in our environment, predominate in women, with CD, with more extensive disease, and greater requirement of biologic therapy. Optimal clinical results were found, such as earlier diagnosis of IBD, low morbimortality, acceptable vaccination rate, access to required therapy, and reduction of complications.
PP-02-073
Impact of the inclusion of a nurse in the multidisciplinary team in inflammatory bowel disease
Viviana Parra-izquierdo and Ginary Orduz and Silvia Hoyos and Juliep Sarmiento and Luis Bohorquez and Diego Cano and Oscar Pinto and Hernan Duarte and Carlos Cuadros and Johon Garces and Juan Acevedo and Andres Barco and Juan Frias and Cristian Florez
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: the nurse becomes the first contact with the patient, favoring empathy, the delivery of education, specific treatment indications and continuous follow-up. For this reason we want to describe the role played by the head nurse in a certified center of excellence in Inflammatory Bowel Disease.
Objective: To describe the interventions carried out by the head nurse of the center of excellence in Inflammatory Bowel Disease to improve the quality of life of patients with this condition.
Materials and Methods: Descriptive cross-sectional study in which the activities carried out by the nurse of the center of excellence since September 2022, date on which the certification was received, are described.
Results: Within the strategy of the center of excellence, the role of the nurse is fundamental, carrying out educational and care work with accompaniment and follow-up for both patients and their families, with the aim of having an impact on improving their quality of life. Likewise, the nurse of the Center of Excellence is part of the Intestinal Ultrasonography days in order to perform a less invasive follow-up of the disease, being the only center of excellence in the country to perform this technique.
Conclusions: In our Center of Excellence the nurse has a fundamental role in the creation, execution and follow-up of the educational, research and assistance programs that are developed in patients with this disease.
PP-02-075
Edema index as a more sensitive indicator of nutritional status with Crohn’s disease
Yu Peng, Yong Li, Ziheng Peng, Duo Xu, Keke Tang and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To confirm whether the edema index could be a nutritional assessment for Crohn's disease (CD) patients.
Materials and Methods: The study was conducted at Xiangya Hospital Central South University between July 2023 and December 2023, including CD patients and the control group. In both groups, bioelectrical impedance analysis was performed, and the edema index defined as extracelluar water/total body water (ECW/TBW) was calculated. The demographic data and body composition parameters were collected through the medical system. The control group consisted of healthy adults, strictly matched for gender, age, and BMI in a 1:1 case-control manner. Univariate analysis and multivariate analysis were used to compare the differences in nutrition-related indicators between both groups.
Results: A total of 126 subjects were included in the final cohort with 50% each of CD patients and healthy individuals. There were no significant differences in gender, age, BMI. Compared to the control group, CD patients had significantly lower fat mass (9.26 ± 5.38 vs.16.63 ± 10.67, P = 0.006), bone mass (2.16 ± 0.41 vs. 2.52 ± 0.57, P = 0.019), but a higher level of edema index (41.07 ± 2.38 vs. 38.53 ± 5.62, P = 0.015). Multivariate logistic regression analysis revealed that edema index was an independent factor to malnutrition in CD patients (OR: 1.665, 95% CI: 1.158-2.395, P = 0.006).
Conclusion: A higher edema index in CD patients demonstrates their worse nutritional status compared to healthy subjects. The edema index may be a sensitive indicator to assess nutritional status in CD patients.
PP-02-076
Platelet-to-albumin ratio: a novel indicator for assessing severity and predicting future surgery in Crohn's disease
Yu Peng, Duo Xu, Yong Li, Ziheng Peng and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The purpose of this study is to find a reliable indicator for assessing Crohn's disease (CD) severity and predicting the probability of Gastrointestinal (GI) surgery.
Materials and Methods: In this study, patients with CD who were hospitalized at the Department of Gastroenterology, Xiangya Hospital, Central South University between January 2017 and June 2022 were included. All data were retrieved from the electronic medical record system. Seven integrated indices were calculated to assess CD severity and predict the probability of future GI surgery in patients with CD. Mann-Whitney U test, logistics regression analysis, Cox regression analysis, and receiver operating characteristic (ROC) curve analysis were used. A p-value of less than 0.05 was considered statistically significant.
Results: A total of 320 patients with CD were included in this study, with 215 having active disease and 105 having moderate-to-severe CD activity. The platelet-to-albumin ratio (PAR) was found to have a significant independent association with CD activity in both univariate and multivariate analyses. The optimal PAR cutoff values of 8.075 and 9.325 could be used to predict CD activity and moderate-to-severe activity, respectively. The following retrospective study on 215 patients revealed that high PAR could predict the probability of future surgery in patients with CD and B2/B3 disease behavior, with 9.325 being the optimal cutoff value.
Conclusion: PAR could be used to assess CD severity as well as predict the probability of GI surgery in patients with CD and B2/B3 disease behavior.
PP-02-077
Bio-naïve patients had higher response rates at weeks 8 with Ustekinumab: retrospective study in China
Yu Peng and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Obejectives: To evaluate the real-world short-term efficacy of Ustekinumab (UST) in Chinese Crohn’s disease (CD) patients after its recent approval in 2020.
Materials and Methods: Retrospective analysis of data from resident patients diagnosed with CD at Xiangya Hospital from July 2020 to May 2023 was performed. The inclusion criteria were moderate-to-severe CD, diagnosed according to the Chinese diagnosis consensus in inflammatory bowel disease, and treatment with UST. Clinical data were collected from the patients’ medical records.
Results: 112 patients analyzed in the study, including 87 males (77.7%) and 25 females (22.3%), the mean age was 30.7±4.7 years. The most frequent disease type(location) was ileocolonic disease (L3, 63.7%). The most frequent disease behavior was stricturing non-penetrating type according to the Montreal Classification (B2, 47.5%). Most patients (58%) had not received biologics therapy. The clinical response rates at weeks 8 was 69%. Regarding the history of biologics therapy, the response rate was significantly higher in bio-naïve patients than bio-failure patients at week 8 (82.1% vs. 56.7%, p<0.05). The clinical response rates at weeks 8 were not correlated to the patient's age, sex, disease location, disease behavior, presence of perianal disease, CDAI, blood tests, and was correlated to the history of biologics therapy. There was no significant difference in the baseline clinical characteristics between the bio-naïve and bio-failure groups.
Conclusion: Bio-naïve patients had higher response rates at weeks 8 with UST which support the short-term effectiveness of UST in Chinese CD patients in real-world practice.
PP-02-078
Impact of Crohn’s disease on working life: Discovering the truth in Chinese patients
Yu Peng, Yong Li, Ziheng Peng, Duo Xu, Keke Tang and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To obtain an eastern perspective of the impact of Crohn's disease (CD) on patients’ working lives.
Materials and Methods: A cross-sectional survey was performed on patients with CD who were hospitalized at Xiangya Hospital of Central South University from January to October in 2023. Data were analyzed using Chi-squared test, Logistic regression.
Results: A total of 356 patients responded the validated questionnaire and all of them met the requirements and were used for analysis. Among the respondents, 273 (76.7%) male and 83(23.3%) female. The career change was related to the following factors: 1) educational level (p < 0.05);2) income of patients after onset (p<0.05); 3) c. the changing of occupational promotion after onset (p < 0.001);4) the impact of finding a new career (p < 0.005); 5)occupational or job discrimination (p < 0.005). Other variables have no significant correlation with this outcome. Based on multivariate logistic regression, the independent risk factors for career change in Chinese Crohn's disease patients were income of patients after onset (odds ratio (OR): 6.226, 95% confidence interval (CI): 0.090-0.749, p=0.013), and the changing of occupational promotion after onset (OR: 7.069 95% CI: 0.105-0.711, p=0.008).
Conclusion: This is one of the few surveys on the impact of CD on professional life of chinese patients. Overall, CD had a strong negative impact on professional life. CD affects career changes, it restricts the access to occupational promotion after onset, and further influence on family life. This issue should be systematically investigated to develop adapted measures.
PP-02-079
Diagnosis and management colitis tuberculosis in severe malnutrition patient
Sabilla Laras Permana and Deka Larasati
Department of Internal Medicine, Gatot Soebroto Army Central Hospital, Jakarta Pusat, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Tuberculosis (TB) can present in various forms, including colitis tuberculosis, which may lead to severe malnutrition. Malnutrition is associated with a twofold higher risk of dying in patients with tuberculosis (TB) and considered an important potentially reversible risk factor for failure of TB treatment.
Case Report: A 20-year-old woman presented with intermittent abdominal pain, diarrhea with blood, a 20 kg weight loss. She had no reported medical history, her sister had a TB pulmonal and had been treated. Initial labs were notable for severe anemia (Hb 6.8), albumin (1.1), and positive Stool GeneXpert assay. Imaging studies showed bilateral pleural effusion and abdominal CT findings suggestive of colitis tuberculosa with associated lymphadenitis. Colonoscopy showed multiple ulcerations in the cecum and ileum, Biopsies confirmed chronic ulcerative colitis with active inflammation TB-PCR assay of the biopsies was positive. Soon after antituberculin therapy was initiated, and alongside nutritional support the patient's condition improved significantly, with resolution of gastrointestinal symptoms and gradual weight gain.
Discussion: This case highlights the importance of recognizing colitis tuberculosis as a potential diagnosis in young patients presenting with gastrointestinal issues, particularly in the context of a family history of tuberculosis. Early diagnosis and appropriate management are crucial to improve outcomes and prevent severe complications. Optimizing nutrition has demonstrated beneficial effects on prognosis and the response to drug therapy for patients with TB, although the evidence is limited and heterogeneous.
PP-02-080
Tuberculosis in patients with inflammatory bowel disease: a case series from Singapore
Tian Yu Qiu and Jeannie Peng Lan Ong and Tiing Leong Ang and Lai Mun Wang and Chin Kimg Tan
Singhealth, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background/objective: Tuberculosis (TB) pose significant health concern among patients with Inflammatory Bowel Disease (IBD) especially those on anti-tumour necrosis factor (anti-TNF). This case series aim to describe the varied presentations of TB in IBD patients in Singapore, a TB endemic country.
Methods: This is a case series of 4 IBD patients on follow-up with Changi General Hospital (Singapore) and diagnosed with TB in 2023. Demographics, IBD treatment, TB diagnosis and subsequent treatment were collected (Table 1).
Results: Patient 1 had Crohn’s Disease (CD) on Adalimumab. TB quantiferon was negative pre- treatment but he developed disseminated TB after 12 months of anti-TNF.
Patient 2 was on Adalimumab for IBD-unclassified. He had latent TB infection despite initial negative TB quantiferon.
Patient 3 had Ulcerative Colitis (UC) on Azathioprine. He was in endoscopic remission when diagnosed with pulmonary TB. He stopped Azathioprine and developed Acute Severe Ulcerative Colitis (ASUC) 2 month later.
Patient 4 was initially diagnosed as ileal CD with endoscopy findings of terminal ileal (TI) ulcer. She received 8-weeks of Budesonide. Repeat colonoscopy for persistent symptoms showed persistent TI ulcer, acid-fast bacilli on Ziehl-Neelsen stain confirmed TB ileitis.
First two cases highlight the risk of TB reactivation on anti-TNF despite initial negative screening. TB gut may also mimic IBD endoscopically. It is crucial to repeat endoscopy for IBD patients who are not responding to initial therapy.
PP-02-081
Mixed-Strain Probiotics Reduce Colitis Severity in DNBS-Induced Colitis Rats
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Inflammatory bowel disease (IBD) is characterized by chronic, relapsing inflammation influenced by genetic, microbial, and environmental factors. This study aims to determine the effectiveness of multi-strain probiotics in reducing colitis severity in rats, assessing clinical responses, histopathology, and inflammatory mediators.
Materials and Methods: Forty male Sprague-Dawley rats, aged six weeks, were used and divided into four groups: Control, Acute Colitis (AC), RP (treated with Lactobacillus rhamnosus and Lactobacillus paracasei), and SG (treated with Lactobacillus salivarius and Lactobacillus gasseri). Probiotics were administered via gavage daily, starting three days before acute colitis induction with rectal DNBS solution and continuing for seven days. On day eight, rats were euthanized for colon and blood sample collection for further evaluation.
Results: The AC group exhibited significantly elevated TNF-α levels compared to Control, RP, and SG groups (p < 0.001). RP and SG treatments significantly reduced TNF-α levels compared to AC (p = 0.002, p < 0.001, respectively). Trends indicated increased IL-10 levels and decreased MDA levels in RP and SG groups, though not statistically significant (Table 1). Histopathological analysis showed a lower median Ameho’s score in the RP group compared to AC, without statistical significance.
Conclusion: Multi-strain probiotics significantly reduced TNF-α levels, suggesting an anti-inflammatory effect in colitis. Trends in IL-10 and MDA levels and histopathological improvements, though not statistically significant, indicate potential benefits. Further research with larger sample sizes is needed to confirm these findings and explore probiotics as a therapeutic option for IBD.
PP-02-082
Changes in trimethylamine-N-oxide levels in ulcerative colitis: relationship with clinical and laboratory indicators
Доктор Nadezhda Samoilova-Bedych and Yelena Laryushina and Lyudmila Turgunova and Alexander Marchenko and Adina Igenbekova
NCJSC "Karaganda Medical University", Karaganda, Kazakhstan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: to compare the level of TMAO in healthy individuals and patients with ulcerative colitis and to consider the possibility of using TMAO as a biomarker in the diagnosis of ulcerative colitis based on its changes on different clinical, laboratory, and endoscopic activity of the disease.
Material and methods: the study included 63 patients with ulcerative colitis and 38 healthy individuals, who were measured TMAO levels by high-performance liquid chromatography with mass spectrometry.
Results: the results showed a statistically significant decrease in TMAO levels in patients with ulcerative colitis compared to the healthy group (p<0.0001), TMAO differed in clinically active and inactive disease (p=0.003). Additional analysis showed a correlation between TMAO levels and clinical-laboratory parameters- age (r=0.377, p=0.003), stool frequency (r=-0.427, p=0.001), WBC (r=-0.31, p=0.042), serum albumin (r=0.379, p=0.002) and fecal calprotectin (r=-0.314, p=0.022): TMAO significantly correlated with disease activity according to the Montreal scale (r=-0.389, p=0.002) and severity of attack according to Truelove-Witts (r=-0.301, p=0.027).
Conclusion: the results emphasize the potential role of TMAO as a biomarker for diagnosis and assessment of ulcerative colitis activity. Further studies are needed to confirm these observations and develop standardized protocols for using TMAO in clinical practice.
PP-02-083
Changes in the expression of lipopolysaccharide-binding protein in patients with ulcerative colitis and Crohn's disease.
Доктор Nadezhda Samoilova-Bedych and Yelena Laryushina and Lyudmila Turgunova and Alexander Marchenko and Aliya Zhirenbayeva
NCJSC "Karaganda Medical University", Karaganda, Kazakhstan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: the study aims to investigate changes in the expression of lipopolysaccharide-binding protein (LPB-protein) in patients with ulcerative colitis (UC) and Crohn's disease (CD) at different disease activities.
Material and methods: an observational cross-sectional study was conducted at the University Hospital of NCJSC "KMU". 112 patients with IBD (63 with ulcerative colitis and 48 with Crohn's disease) and 41 healthy individuals were analyzed for LPS-protein concentration by enzyme-linked immunosorbent assay.
Results: differences in LPB-protein expression levels were obtained in patients with UC and CD compared to the control group (p=0,05). At the same time, the median in the group with UC (Me 16.8 ng/mL) was higher than in patients with CD (Me 14.2 ng/mL). LPB-protein index had no statistically significant changes depending on the lesion extent, clinical and laboratory activity according to Trulove-Witts and CDAI scales, Meyo and Harvey-Bradshaw indices, endoscopic activity in UC and CD.
Conclusions: our data indicate the presence of changes in the expression level of LPS-binding protein in patients with UC and CD compared to healthy individuals. This suggests the presence of changes in the microbiome in patients with IBD in favor of a predominance of Gram-negative flora with greater expression in UC. However, for more in-depth study of LPB-protein changes at the different activity of UC and CD, additional studies are needed.
PP-02-084
Inhibition of IL-17 signaling induces intestinal mucosal inflammation
Yosuke Shimodaira, Sho Fukuda, So Takahashi and Katsunori Iijima
Akita University Graduate School Of Medicine, Akita City, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: IL-17 is a key cytokine in various inflammatory disease. Targeting IL-17 signals blockage did not improve inflammatory bowel disease (IBD) clinically, but also even developed de novo enteritis in non-IBD subjects. Little is known about the effects of blocking IL-17 signaling on intestinal homeostasis. This study investigated intestinal immunity under the inhibition of IL-17 signaling.
Methods: Refractory patients with psoriasis who started receiving anti-IL-17 or anti-IL-17 receptor inhibitors were included in this study. Evaluation for fecal immunochemical test (FIT), fecal calprotectin (fCal), endoscopic findings, colonic mucosa histology, and fecal microbial composition before and 3 months after starting treatment on the subjects was performed. Fecal microbial composition was analyzed with 16S rRNA.
Results: Five psoriasis patients received antibodies. The median FIT level and fCal level was 0 ng/mL (SD 162) and 39.4 mg/kg (SD 180) at baseline. Thereafter, the median FIT level and fCal level on 3 months after initiating antibody therapy increased to 19 ng/mL (SD 136) and 222 mg/kg (SD 141), respectively. Endoscopic findings before administration showed mild edema in one patient and mild redness in one patient, and thereafter mild edema in two patients and mild redness in two patients after administration. The number of mononuclear cells infiltrating in the mucosa significantly increased with antibody administration. Microbial community analysis showed alteration in alpha diversity after antibody administration. Proteobacteria was increased after the administration.
Association of Fecal Calprotectin with Ulcerative Colitis Disease Activity and Severity.
Marsela Sina, Afërdita Djegsi, Sara Hoxha, Xhensila Pemaj and Skerdi Prifti
University Clinic of Gastrohepatology, Dept of Internal Diseases, University of Medicine, Tirana, Albania
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We aim to evaluate the association between fecal calprotectin (FC) and Ulcerative Colitis (UC) disease severity.
Materials and Methods: 82 UC patients underwent colonoscopy, from January 2018 to June 2024 at the University Clinic of Gastrohepatology. Laboratory data, clinical and endoscopic scores were used to assess the disease activity.
The data were analyzed by independent test, correlation test and receiver operating characteristic (ROC) curve analysis. A p<0.05 was considered statistically significant.
Results: 38 (46.3%) male and 44 (53.7%) female patients were included in the study. Mean age was 42.46 ± 13.2 years. 2 (2.4%) patients had ulcerative proctitis; 44 (53.7%) left-sided colitis, 36 (43.9%) patients had pancolitis. Mean disease duration was 6.63 ± 6.76 years. 30.5% of patients were in remission, while 69.5% had active disease.
We found a significant positive correlation between FC and partial Mayo Score (r=0.364, p=0.001); FC and endoscopic Mayo score (r=0.338, p=0.002), FC and total Mayo score (r=0.374, p=0.001); and FC and Ulcerative Colitis Endoscopic Index of Severity (UCEIS) Score (r= 0.368 p=0.001). A significant association was found between FC value and severity of disease (p= 0.008). In multiple regression analysis, FC significantly statistically correlates only with UCEIS Score (p=0.015).
The optimum cut-off values of FC reflecting active UC was 162.18ug/g with 83.6% sensitivity and 39.1% specificity. (AUC 0.825, 95%CI: 0.717-0.934).
Conclusion: Our study showed strong correlations between FC levels and disease severity scores in UC. A FC cut-off of 162.18μg/g, was 83.6% sensitive for detecting active disease, with an AUC of 0.825.
PP-02-086
Risk factors of low bone density in inflammatory bowel disease patients
Marsela Sina, Sara Hoxha, Afërdita Djegsi and Adriana Babameto and Skerdi Prifti
University Clinic of Gastrohepatology, Dept of Internal Diseases, University of Medicine., Tirana, Albania
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate possible risk factors of low bone density among our Inflammatory Bowel Disease (IBD) patients.
Materials and Methods: This is a prospective study conducted at the University Hospital Center Mother Teresa, on IBD patients who underwent Bone Mineral Density (BMD) assessments with dual-energy X-ray absorptiometry scans from January to December 2023. Demographic, laboratory data, disease duration, activity and treatment were collected for each patient.
Results: 36 IBD patients, mean age 45.86 ± 15 years; 33.3% males; 66.7% females. 16.7% Crohn’s Disease and 83.3% Ulcerative Colitis patients were included in the study. 91.7% were non-smokers; 33.3% on corticosteroid therapy; 66.6% on biologic treatment; 16.6% were taking vitamin D, and 8.3% patients underwent ileo-colic resection.
The prevalence of osteopenia and osteoporosis was 38.9% and 27.8% respectively. 36.1% of cases had osteopenia at the lumbar spine, 33.3% of cases at the femoral neck. 22.2% of cases had osteoporosis at the lumbar spine and 5.6% of cases at the femoral neck.
A strong positive correlation was found between low lumbar T score and age (r=0.618, p<0.01), female gender (r=0.362, p=0.034) and steroid treatment (r=0.408, p=0.014). A strong positive correlation was found between low femoral T-score and age (r=0.459, p=0.005) and disease duration (r=0.347, p=0.038). A strong negative correlation was found between biological treatment and low lumbar T score (r=-0.351, p=0.036). No correlation was found between low BMD and type of IBD disease.
Conclusion: In our patients, low BMD is correlated to older age, female gender, disease duration and corticosteroid therapy.
PP-02-087
Which inflammatory biomarkers are better predictors of UC activity? Our Experience
Marsela Sina, Afërdita Djegsi, Sara Hoxha, Xhensila Pemaj and Skerdi Prifti
University Clinic Of Gastrohepatology, Dept. of Internal Disease, University of Medicine, Tirana, Albania
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: In clinical practice, there are several biomarkers used to monitor ulcerative colitis (UC) disease activity. We aim to evaluate which inflammatory biomarkers are better to predict disease activity in (UC) patients.
Materials and Methods: This is a prospective study of 82 cohort UC patients who underwent endoscopic evaluation, from January 2018 to June 2024 at the University Clinic of Gastrohepatology, University Hospital Center Mother Teresa, Tirana. Demographics, laboratory data and Ulcerative Colitis Endoscopic Index of Severity (UCEIS) score was collected for each patient. UC was considered active when UCEIS >2 points.
Results: 38 (46.3%) male and 44 (53.7%) female patients were included with a mean age of 42.46 ± 13.2 years. 2 (2.4%) patients had ulcerative proctitis; 44 (53.7%) patients had left-sided colitis and 36 (43.9%) patients had pancolitis. 25 (31%) patients were in remission 57 (69%) had active disease. Mean disease duration was 6.63 ± 6.76 years.
We found a significant positive association of fecal calprotectin (FC) (r=0.368, p=0.001); WBC (r=0.457, p<0.001); neutrophil/lymphocyte ratio (r=0.418, p=0.001); CRP (r=0.396, p=0.001); CRP/albumin (r=0.436, p=0.002), and ferritin (r=0.250, p=0.039) with UCEIS score. A significant negative association was found between UCEIS score and lymphocyte/monocyte ratio (r=-0.324, p= 0.004) and albumin (r=-0.486, p=0.001).
In multiple linear regression analysis significant predictors included FC (β=0.687, p = 0.015), CRP (β = 0.416, p = 0.011), and the CRP/Albumin ratio (β = -4.491, p = 0.015).
Conclusion: These findings demonstrate that FC, CRP and CRP/Albumin are important inflammatory biomarkers in order to predict UC activity.
PP-02-088
The simple non-invasive markers as the potential markers in inflammatory bowel disease
1Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia;2Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Inflammatory bowel disease (IBD) is caused by multifactorial, one factor is an abnormal immune response to gut microflora. The neutrophil-to-albumin ratio (NAR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), erythrocyte sedimentation rate (ESR), are the simple non-invasive systemic inflammatory markers in various medical condition, including inflammation disease, blood vessel disorder and cancer.
Objective: To evaluate the simple non-invasive markers as diagnostic potential marker in IBD patients.
Method: This was case control study using electronic medical record at H. Adam Malik Hospital. A total of 36 samples of IBD and healthy population as control group were included in the study. Clinical characteristics, NAR, NLR, PLR, ESR were collected.
Results: The most clinical manifestations were stomachache and bloody stools. IBD patients suffered anemia, hypoalbuminemia, as well significant differences in NLR, PLR, ESR compared to with control with p value < 0.001. There was significant differences between NAR, NLR, PLR, ESR in the IBD group with control. The receiver-operating characteristic (ROC) analysis revealed the optimal cutoff of NAR to predict IBD was 13,89, with sensitivity and specificity of 88.9% and 88.9%, respectively. For NLR, the best cut-off value was 2.33, with sensitivity and specificity of 83.3% and 83.3%, respectively. For PLR, the best cut-off value was 140.573, with sensitivity and specificity of 83.3% and 83.3%, respectively. For ESR, the best cut-off value was 23.5, with sensitivity and specificity of 94.4% and 94.4%, respectively.
Conclusion: NAR, NLR, PLR, ESR as simple non-invasive markers could be used to predict diagnosis of Inflammatory Bowel Disease.
PP-02-089
Inflammatory bowel disease is associated with attenuated hepatic steatosis by Fibroscan
Yeo Won Sohn1, Kwang Woo Kim1, Bo Kyung Kim1, Hee Jun Jang1, Dong Kee Jang1, Seong Joon Koh2, Su Hwan Kim1, Hyoun Woo Kang1, Yong Jin Jeong1, Byeong Gwan Kim2, Kook Lae Lee1 and Ji Won Kim1
1Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, South Korea;2Seoul National University Medical Center, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study is designed to find out relationship between IBD and either hepatic steatosis or liver fibrosis scanned by Fibroscan.
Materials and Methods: This is a prospectively enrolled, cross-sectional and single-center observational study. Subjects with inflammatory bowel disease who have been treated between June 2018 and April 2024 were enrolled. Subjects planned for health screening during the same period were matched as the control group. Hepatic steatosis and fibrosis were evaluated with controlled attenuation parameter (CAP) and liver stiffness measurement (LSM) by Fibroscan, respectively.
Results: 279 subjects were enrolled. 142 and 137 subjects were in the group of IBD and control, respectively. IBD group showed significantly less CAP score than that of the control group. (215.8 ± 54.2 vs. 247.9 ± 51.9, p < 0.001) However, there was no significant difference in LSM score between the IBD and control group. (4.1 ± 1.4 vs. 4.7 ± 6.2, p = 0.312) Both ulcerative colitis (UC) and Crohn’s disease (CD) group showed consistent tendency when compared with the control group. (247.9 ± 51.9 vs 220.4 ± 54.4, p < 0.001; 247.9 ± 51.9 vs 205.3 ± 52.8, p < 0.001, respectively) The multivariable analysis showed that IBD was still inversely associated with hepatic steatosis (adjusted odds ratio 0.506, 95% confidence interval 0.293 - 0.876, p = 0.015).
PP-02-090
CT/MR Enterography: Utilization in Filipino patients with Inflammatory Bowel Disease
Jose Luis Matthias Sollano
Makati Medical Center, Makati, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Methods: Researchers retrospectively analyzed data over 10 years from patients with suspected or diagnosed IBD who underwent MRE or CTE. Patients with known GI tuberculosis, colorectal cancer, or prior abdominal surgeries were excluded. MRE and CTE findings were suggestive of IBD were recorded and compared.
Results: Fifty patients were included, with 32 undergoing MRE and the rest having CTE. MRE and CTE detected additional small bowel lesions in 90.9% of patients with known CD. Common findings included wall thickening and pericolic fat stranding. Similarly, in 93.3% of suspected IBD cases, MRE and CTE identified small bowel involvement compatible with CD.
Conclusion: This study highlights the real-world use of MRE and CTE in managing IBD, particularly CD, in the Philippines. The additional information obtained from these procedures aids in accurate diagnosis and comprehensive management of Filipino IBD patients.
PP-02-091
Comparison of Ustekinumab and Vedolizumab for Moderate-to-Severe UC with Prior Failure of Biologics or SMDs
Seung Min Hong1, Dong Hoon Baek1, Geun Am Song1, Dong Chan Joo1, Tae In Kim1, Gwang Ha Kim1, Bong Eun Lee1, Cheolung Kim1 and Hyeon Tae Cho2
1Department of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea;2Centum Medihill Hospital, Busan, Republic of Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study compared the effectiveness of Ustekinumab (UST) and vedolizumab (VDZ) in patients with moderate to severe ulcerative colitis who have previously failed treatment with biologics or small molecule drugs (SMDs).
Materials and Methods: The study retrospectively analyzed the medical records of a total of 43 patients with moderate to severe ulcerative colitis, comprising 15 patients in the UST group and 28 in the VDZ group. All of these patients had previously experienced failure with treatments involving biologics or SMDs.
Results: At week 52 following the initiation of UST or VDZ treatment, there were no statistically significant differences between the two groups in terms of the clinical remission rate (UST group 60.0% vs. VDZ group 67.9%, p = 0.606) and the corticosteroid-free remission rate (UST group 46.7% vs. VDZ group 57.1%, p = 0.512). At the time of response evaluation following the induction phase, the proportion of patients showing an endoscopic response did not demonstrate a statistically significant difference between the two groups (UST group 80.0% vs. VDZ group 78.6%, p = 1.000). However, at week 52, the drug survival rate was significantly higher in the UST group at 93.3% compared to 71.4% in the VDZ group (p = 0.031). There were no significant differences in adverse events between the two groups.
Conclusion: In patients with moderate to severe ulcerative colitis who had previously failed treatment with biologics or small SMDs, UST demonstrated a superior drug survival rate compared to VDZ.
PP-02-092
Usefulness of Underwater EMR for neoplastic lesions in ulcerative colitis
1Center For Diagnostic And Therapeutic Endoscopy, Keio University School Of Medicine, Tokyo, Japan;2Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan;3Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Tokyo, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: According to Western guidelines, endoscopic resection (ER) is acceptable for neoplastic lesions with clear boundaries, even within areas affected by ulcerative colitis (UC), however, this procedure is also difficult due to advanced fibrosis of mucosa associated with previous inflammation within areas affected by UC. Underwater endoscopic mucosal resection (UEMR)has become popular in recent years and has been useful for lesions with advanced fibrosis. In this study, we verified the usefulness of UEMR for lesions diagnosed as sporadic neoplasms (SN) before ER, among neoplastic lesions that developed within areas affected by UC.
Material and Methods: We enrolled 35 lesions in 26 patients that underwent UEMR for neoplastic lesions 20 mm or smaller in diameter, which had developed within areas affected by UC, from December 2021 to February 2023 at Keio University Hospital. The primary endpoint was R0 resection rate, while the secondary endpoints were en bloc resection rate, procedure time. and adverse events.
Results: Background mucosa consisted of normal mucosa (regenerated mucosa after inflammation) in nine lesions (25.7%), atrophic mucosa in 20 lesions (57.1%), and atrophic mucosa with ulcer scars in six lesions (17.2%). A total of 34 lesions (97.1%) underwent en bloc resection; of them, 29 lesions (85.3%) underwent R0 resection, while only one (2.9%) underwent piecemeal resection. In terms of procedural accidents, there were no cases of intraoperative perforation, delayed perforation, post polypectomy coagulation syndrome, and delayed bleeding.
Conclusion: UEMR may be an effective and safe endoscopic resection method for SN within areas affected by UC.
PP-02-093
Risk factors and response predictors of iron deficiency without anemia in patients with IBD
Sunil Thomas George, Rajesh Gopalakrishna, Priya Nair, Sharon Paul Methala, Anoop K Koshy and Shine Sadasivan
Amrita Institute of Medical Sciences, Kochi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To determine the prevalence of iron deficiency without anemia (IDWA) in patients with inflammatory bowel disease (IBD) and to evaluate the effects of iron supplementation.
Materials and Methods: Our prospective interventional non-randomized study was conducted over 12 months in 94 consecutive patients with confirmed IBD. Clinical and biochemical parameters including complete blood count, serum ferritin, serum iron, transferrin saturation (TFS) and C-reactive protein, were recorded. Health-related quality of life (HRQoL) was assessed using IBDQ-9 and FACIT-F questionnaires. Iron deficiency was diagnosed on basis of serum ferritin levels (<100 μg/L with active inflammation or <30 μg/L without inflammation) and TFS <16%. Iron supplementation was administered; and parameters were reassessed at one and three months. Statistical analyses included Chi-square tests, multivariate binary logistic regression, and paired sample t-tests.
Results: We found that 55(58.6%) of IBD patients were iron deficient. The iron-deficient group had a higher proportion of women (59%) compared to men (41%). Among the iron-deficient group, the baseline mean hemoglobin level was 12 g/dL which improved to mean of 14 g/dL by the third month following iron supplementation. The hemoglobin levels in the normal group remained stable throughout the study period. Iron deficiency was seen to have a significant impact on quality of life.
Conclusion: Iron deficiency without anemia is a common and clinically significant condition in IBD patients, adversely affecting their quality of life. Screening for iron deficiency and timely iron supplementation leads to significant improvements in hemoglobin levels and enhances overall quality of life.
PP-02-094
Macrophage-Derived Asparagine Promotes Fibroblast Proliferation and Drives Intestinal Fibrosis
Yiwen Tu, Haiming Zhuang, Yubei Gu, Yao Zhang and Duowu Zou
Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School Of Medicine, Shanghai, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study investigates the reprogramming of amino acid metabolism in intestinal fibrosis, focusing on the metabolic interaction between macrophages and fibroblasts via asparagine.
Materials and Methods: Metabolomics were used to measure metabolite levels in normal and fibrotic intestinal tissues from Crohn's disease patients (n=122). Metabolic tracing using C13- and N15-labeled asparagine and aspartate was conducted in mice and human primary cells both in vitro and in vivo. Co-culture experiments of primary macrophages and fibroblasts were performed. Gene expression was investigated using qRT-PCR, Western Blot, and immunofluorescence. Col1a2Cre ASRGL1flox mouse model was constructed to explore the potential of targeting asparaginase in treating intestinal fibrosis.
Results: Crohn's disease patients showed notable metabolic differences between normal and fibrotic tissues, with amino acid metabolism being the most affected. Amino acids were generally downregulated in fibrotic areas, except for a significant increase in aspartate (p<0.01). Both synthesis and degradation of asparagine were markedly elevated in fibrosis (p<0.05). Single-cell transcriptomics suggested that asparagine synthetase (ASNS) in macrophages and asparaginase (ASRGL1) in fibroblasts were upregulated during fibrosis. This was confirmed by in situ immunofluorescence and qPCR. In vitro, TGF-β enhanced asparagine production in macrophages, and ASNS knockout reduced fibroblast proliferation. C13-labeled asparagine tracing showed fibroblasts use it for nucleotide synthesis. Conditional ASRGL1 knockout significantly reduced fibroblasts proliferation and alleviated intestinal fibrosis.
Conclusion: In intestinal fibrosis, fibroblasts uptake excess asparagine synthesized by macrophages and utilize asparaginase to convert it into aspartate for nucleotide synthesis, thereby promoting their proliferation. Targeting asparagine catabolism could alleviate intestinal fibrosis in mice.
PP-02-095
AXL-dependent autophagy impairment differentiates monocyte-derived macrophages from Crohn’s disease and intestinal tuberculosis.
Sonakshi Udinia and Mrinmoy Das and Shaina Jamwal and Manasvini Markandey and Aditya Bajaj and Lalita Mehra and Saurabh Kedia and Carey F. H. Lim and Shihui Foo and Prasenjit Das and Shanshan Howland and Mrutyunjay Suar and Amit Singhal and Vineet Ahuja and Dhiraj Kumar
International Centre For Genetic Engineering And Biotechnology, New Delhi, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Macrophages are critical to maintain intestinal homeostasis and contribute to localized inflammation once dysregulated. Here, we aimed to understand innate macrophage dysfunction in the subjects with chronic idiopathic gut inflammation [Crohn’s Disease (CD)] and chronic infection-related gut inflammation [Intestinal Tuberculosis (ITB)].
Materials and Methods: RNA-seq analysis of ex vivo Mtb-infected or uninfected monocyte-derived macrophages (MDMs) from 8 CD, 5 ITB and 5 controls was done. Subsequently, MDMs from a cohort of patients, including CD (n=42) and ITB (n=41) were characterised for autophagy (CD, n=15: ITB, n=09), mitochondrial depolarization (CD, n=08: ITB, n=08), and bactericidal capacity (CD, n=25: ITB, n=28), at basal state or in response to ex vivo Mtb infection. Finally, the role of Axl downregulation in impaired autophagy was tested.
Results: RNA-seq analysis of MDMs from CD and ITB subjects revealed differential regulation of autophagy-associated pathway genes either basally or upon ex vivo Mtb infection. CD MDMs also showed significantly impaired autophagy compared to ITB MDMs. MDMs from CD and ITB subjects were permissive to Mtb uptake; however, CD MDMs allowed relatively better survival. We noted that AXL was consistently downregulated in CD MDMs compared to ITB, which was also reflected in the biopsies. AXL knockdown in Thp-1 macrophages impaired autophagy flux, suggesting a causal relationship between reduced AXL expression and impaired autophagy in CD MDMs.
Conclusion: Autophagy impairment can explain the heightened chronic inflammation observed in CD subjects. Identifying signatures associated with autophagy impairment could facilitate easy diagnostic and novel intervention approaches against CD.
PP-02-096
Clinical Characteristics and Treatment Outcomes of Isolated Perianal Crohn's Disease
Wasuwit Wanchaitanawong1,2, Marianee Salaemae2, Varut Lohsiriwat3 and Julajak Limsrivilai2
1Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand;2Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand;3Division of Colon and Rectal Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To compare characteristics among patients with isolated perianal Crohn's disease (ipCD), perianal Crohn's disease with luminal disease (pCD), and recurrent cryptoglandular disease (CGD), and to compare treatment outcomes between ipCD and pCD patients.
Materials and Methods: We performed a retrospective cohort study of consecutive patients with complex perianal fistula (CPF) confirmed by pelvic MRI at Siriraj Hospital. ipCD was defined as recurrent or refractory perianal fistula within 6 months post-surgery, without evidence of luminal disease, and responsive to biologic treatment.
Results: Of 32 CD with perianal fistula and 33 recurrent CGD, 33 had MRI-confirmed CPF (9 ipCD, 9 pCD, 15 CGD). Compared to pCD, ipCD patients were older at diagnosis (38 vs 27 years,p=0.020), had longer disease duration (64 vs 8 months,p=0.006), higher hemoglobin (13.9 vs 11.1 g/dL,p<0.001), lower fecal calprotectin (268 vs 2498 mg/kg,p=0.014), and more complex fistula type (88.9% vs 28.6%,p=0.031). Compared to CGD, ipCD patients were younger at diagnosis (38 vs 47 years,p=0.032), had more branched fistula (88.9% vs 46.7%,p=0.011), internal opening located in a deeper position from the anal verge (3.1 vs 1.2 cm,p=0.005), and higher rate of associated abscess formation (88.9% vs 46.7%,p=0.011). ipCD patients had lower 6-month fistula remission after biologic treatment than pCD (11.1% vs 100%,p=0.007).
PP-02-097
Rescue Treatment of Ulcerative Colitis in Patients Unresponsive to Biologics and Recent Small Molecule treatment
Stephen Wolman and Victoria Boquiren
Toronto General Hospital, Toronto, Canada
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Many patients with ulcerative colitis respond to 5-ASA, infliximab, ustekinumab, vedolimumab, risankizumab, mirikizumab, tofacitinib or upacitinib. Still, some patients do not respond to these medications as single agents.
Case Report: In three patients who have pancolitis and have failed single agents, we tried combination therapy. We chose a biologic that had some impact, although minimal, and combined it with an oral JAK inhibitor. We chose the biologic with low risk of infection side effects. Patient #1 has been controlled for 3 years with no side effects. Patients 2 & 3 are only treated for three months with no significant side effect. (See results table)
Discussion: Combinations of low risk biologics combined with JAK inhibitors should be considered in severe ulcerative colitis before colectomy.
PP-02-098
Mitigation effect of Lactococcus formosensis on mice with dextran sulfate sodium induced ulcerative colitis
女士 Qiuyan Wu, Weilong Zhong and Bangmao Wang
Tianjin Medical University General Hospital, 天津, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Lactococcus Formosan is a bacterium isolated from the liver and lymph nodes of patients with severe constipation and belongs to the genus Lactococcus. The aim of this study was to investigate the role of Lactococcus formosanus in ulcerative colitis and its mechanisms.
Materials and Methods: The colitis model was induced in C57BL/6 mice using dextran sulfate sodium (DSS).Mice were gavaged with sterile PBS or Lactococcus Formosan. Bodyweight, colon length、diarrhea severity, intestinal permeability and colonic histopathology of mice were examined. Real-time qPCR and enzyme-linked immuno sorbent assay were used to detect the content of Inflammatory factors(IL-1β, IL-6, TNF-α, DAO)in mouse serum. The levels of intestinal tight junction related proteins(occludin and ZO-1) in colon tissue were analyzed through Western Blot and immunofluorescent.
Results: Supplementation with Lactococcus formosanus significantly attenuates weight loss and colon shortening, reduces colonic inflammation, ameliorates epithelial damage and enhances intestinal barrier integrity in mice with colitis. Compared to the DSS group, the inflammatory cytokines IL-1β, IL-6, and TNF-α in Lactococcus Formosan group were reduced. In addition, proteins linked to TJ were elevated after Lactococcus formosanus intervention.
Conclusion: This study demonstrate that Lactococcus formosanus effectively alleviated DSS-induced colitis in mice by repairing the mucosal barrier and maintaining the intestinal microecological balance.
PP-02-099
Global Air Pollution and Inflammatory Bowel Disease Incidence: A GBD-based Model Analysis
Xiang Xu1,2, Pengguang Yan1 and Jingnan Li1
1Peking Union Medical College Hospital, China;2Peking Union Medical College, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To establish a mathematical model between air pollutants and IBD incidence, and clarify the impact of air pollution on IBD.
Materials And Methods: Data on annual air pollutant concentrations (household air pollution (HAP), NO2, O3, PM2.5), socio-demographic index (SDI), and age-standardized rate of IBD incidence (ASIR) for 204 countries and regions from 1990 to 2020 were collected from GBD database. Spearman rank correlation analysis was conducted to explore the correlation. A random forest regression model was employed to establish the fitting model.
Results: From 1990 to 2020, the global ASIR of IBD initially increased, reaching a peak of 4.72 cases per 100,000 people in 2010, then declined to 4.45 cases per 100,000 people. ASIR was positively correlated with SDI (r = 0.58) and NO2 (r = 0.53), and negatively correlated with HAP (r = -0.56) (p < 0.01). The random forest regression model exhibited a strong fit to the ASIR, with RMSE, MAE, and MAPE of 0.45, 0.20, and 9.74%, respectively. Feature importance analysis revealed that SDI was the most important factor in predicting ASIR, followed by HAP, PM2.5, O3, and NO2, suggesting that air pollution might influence ASIR, with indoor air quality potentially outweighing outdoor air quality.
Conclusions: This study fills the gap between global air pollution and IBD epidemiology. Based on the random forest regression model, HAP, NO2, O3, PM2.5, and SDI were used to fit the trend of IBD incidence, offering predictive value and a scientific basis for the future study of IBD.
PP-02-100
ABA from B.licheniformis ameliorates DSS-induced colitis in mice by regulating gut microbiota and immune response
Zeyan Xu1, Lijiang Zhao1, Ruihua Shi1,2 and Daqing Gao1
1Southeast University, Nanjing, China;2Zhongda Hospital, Southeast University, Nanjing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
ABA is a secondary metabolic and sesquiterpene product which has been shown modulation in T-cell classification and adhesion molecule expression to ameliorate gut inflammation in experimental mice with DSS-induced colitis. Some evidences suggest that gut microbiota is an important factor in regulating immune responses in IBD. ABA treatment maybe modulate gut microbiota to affect IBD progression. However, the ABA content in a living thing is so very low that it is difficult for ABA to be purified. The bcaba1 gene encodes a cytochrome P450 monooxygenase and is associated with ABA biosynthesis in fungus Botrytis cinerea (B. cinerea). In this study, we explored whether the Bl-cyp strain which capable of enhancing its ABA production, could be more effective in ameliorating DSS-induced colitis by regulating the gut microbiota and host immunity. Our present study provide evidences for the prevention and treatment against IBD.
PP-02-101
Prescription cases of Biologics and Small Molecule Therapies for our ulcerative colitis patients
Yoshiharu Yamaguchi
Aichi Medical University School Of Medicine, Nagakute, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Ulcerative colitis (UC) predominantly impacts the youth, marked by alternating flare-ups and remissions. Despite treatments like biologics (BIO) and JAK inhibitors (JAK-I), a definitive cure for moderate to severe IBD remains elusive. This study examines BIO and JAK-I efficacy and continuation in real-world UC patients, considering the diverse patient backgrounds.
Materials and Methods: We analyzed 81 UC patients treated with BIO (50) and JAK-I (31) from January 2019 to December 2023. Those discontinuing treatment within three months were ‘No responders,’ and those continuing beyond three months but less than a year were ‘Partial responders.’
Results: Non-response rates were 25.9% for BIO and 10.8% for JAK-I, without a significant difference (p=0.108). The BIO group had a significantly higher partial response (40.7% vs. 10.0%, p<0.05). Kaplan-Meier analysis indicated a higher continuation rate for JAK-I. No notable difference in serious adverse events, including cardiovascular issues or herpes zoster incidence, was found between groups.
Conclusion: UC patients on BIO faced more challenges in continuing treatment than those on JAK-I. While serious adverse events were similar for both, proactive measures to reduce risks like thrombosis are recommended.
PP-02-102
Salivary Exosomes in Colitis: Unraveling a Novel Mechanism of Oral-Intestinal Communication
Congyi Yang, Jingyi Chen and Ning Chen
Peking University People's Hospital, Beijing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Inflammatory bowel disease (IBD) involves not just intestinal inflammation but also extraintestinal manifestations, including frequent involvement of the oral cavity.This study aims to unearth the mechanisms underlying long-distance communication between the mouth and gut and the precise role of salivary exosomes.
Materials and Methods: Salivary exosomes were isolated from healthy controls(n=25)and patients with IBD(n=105) using ultracentrifugation. In vivo, we established a colitis mouse model. In vitro, THP-1 cells and Caco-2 cells were exposed to salivary exosomes separately.
Results: Salivary exosomes with DIL tags primarily built up in the mouse colon six hours after gavage. In the DSS mouse model, we observed that salivary exosomes from patients with active IBD (active IBD-Sexos) exacerbated colitis, while those from IBD patients in remission (remission IBD-Sexos) did not. The funding indicates that salivary exosomes may play a possible role in the regulation of macrophage polarization towards the M1 phenotype, disruption of intestinal epithelial function, and alteration of the intestinal flora. Co-culturing THP-1 cells or Caco-2 cells with active IBD-Sexos induces an inflammatory reaction, resulting in an elevated release of proinflammatory cytokines including TNF-a, IL-6, and IL-1β. Additionally, we identified differentially expressed microRNAs in active IBD-Sexos compared to exosomes extracted from IBD patients in remission and healthy control, indicating a unique immune and inflammatory signature associated with active IBD.
Conclusion: Our findings demonstrate the significance of salivary exosomes in bridging the oral and intestinal compartments, independent of microbes or immune cells. Furthermore, it shows that active IBD-Sexos actively promote the progression of colitis.
PP-02-103
Colonic CD4+ T cell senescence contributes to the progression of colitis in aged mice
Yang Zhang, Jun Xu, Shan Cao, Yiken Lin and Yulan Liu
Peking University People's Hospital, Beijing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Aging renders the elderly increasingly susceptible to inflammatory and autoimmune disease. Herein, we sought to determine the role of immunosenescence in age-dependent colitis and to explore the underlying mechanisms.
Methods: Young (2-3 months), middle-aged (10-12 months), and aged (20-24 months) mice were established models of colitis by 2% dextran sulfate sodium salt (DSS) treatment. We detected the senescence-associated β-galactosidase (SA-βGal) activity in lymphocytes isolated from colon of mice. Single-cell and bulk RNA-seq of colonic immune cells in mice with different ages were performed to investigate the precise molecular mechanisms. CD4+CD25-CD45RBhi T cell adoptive transfer model was used to further analyze the role of senescent CD4+ T cells in colitis.
Results: Ageing increased the severity of DSS-induced colitis. The greatest age-associated increases of SA-βGal activity were observed in colonic CD4+ T cells. Aged colonic CD4+ T cells generate higher levels of IFN-γ and IL-17 comparing to young colonic CD4+ T cells, especially in status of colitis. Single-cell analysis revealed that aging increased colonic CD4+ TEM cells, which were associated with T cell-mediated cytotoxicity and cytokine-mediated signaling pathway. Splenic CD4+ TEM cells isolated from aged colitis mice were confirmed to involve in cellular senescence, Th17 differentiation and inflammatory signaling pathway through bulk RNA-seq. Adoptive transfer of aged CD4+ T cells to Rag-/- mice accelerated CD4+ T cell production of inflammatory cytokines and induced more severe colitis compared with young CD4+ T cells transfer.
Conclusion: These results provide a significant insight into the contribution of senescent CD4+ T cells to age-dependent colitis.
PP-02-104
USP9X restrains mucosal inflammation by orchestrating the intestinal monocyte to macrophage maturation via deubiquitinating STAT1
先生 Tao Zhang and Hailong Cao
Tianjin Medical University, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Inflammatory bowel disease (IBD) is chronic inflammatory disorder characterized by the crucial involvement of macrophages. Ubiquitin-specific protease 9X (USP9X) is a deubiquitinating enzyme that regulates protein stability and activity by removing ubiquitin tags from proteins. This study aims to comprehensively understand the molecular mechanisms by which USP9X regulates macrophage function during colonic inflammation.
Materials and Methods: The expression level of USP9X in macrophages of IBD patients was analyzed using sequencing data from single-cell sequencing data. Mice with a macrophage-specific knockout of USP9X were utilized to study the effect of macrophage USP9X on the development of colitis.
Results: USP9X expression was reduced in colonic macrophages of IBD patients. Upon LPS stimulation, USP9X expression significantly decreased in PBMCs, BMDMs, RAW264.7, and THP-1 cells. Knockdown of USP9X led to increased expression of M1 macrophage markers and pro-inflammatory cytokines, enhanced phagocytosis, and migration. In the colitis mouse model, macrophage-specific USP9X deficiency exacerbated colitis and elevated M1 macrophages in the colonic lamina propria.Ubiquitin proteomics revealed that USP9X silence increased ubiquitination levels at K379 and K544 on STAT1. Co-immunoprecipitation and immunofluorescence confirmed the interaction between USP9X and STAT1. USP9X deletion enhanced STAT1 phosphorylation and accelerated its nuclear translocation, which is critical for M1 macrophage polarization.
1The Chinese University Of Hong Kong, Hong Kong, China;2Department of Physics, Boston University, Boston, Massachusetts, 02215, USA;3College of Professional Studies, Northeastern University, Boston, Massachusetts, 02115, USA;4Department of Chemistry, Boston University, Boston, Massachusetts, 02215, USA;5Department of Physics and Astronomy, Northwestern University, Evanston, Illinois, 60208, USA;6Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, Guangdong, 518000, China;7Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100080, China;8Department of Computer Science and Engineering, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, 999077, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Inflammatory bowel disease (IBD) presents a significant global health challenge, exhibiting varied levels and trends across countries and regions. Understanding these geographical disparities is essential for developing effective prevention and treatment strategies.
Methods: This study utilized data from the Global Burden of Diseases Study 2021 to investigate IBD trends across 204 countries and territories from 1990 to 2021. Annual case data and age-standardized rates(ASRs) were analyzed to assess IBD incidence, mortality, and disability-adjusted life-years(DALYs). Total percentage changes were used to track trends in incidence, mortality, and DALY rates over time.
Results: In 2021, the global incidence of IBD was estimated at approximately 0.38 million cases(95% uncertainty interval[UI]: 0.33-0.44 million), resulting in 1.51 million DALYs(95% UI: 1.31-1.75 million). From 1990 to 2021, the global age-standardized incidence rate increased by 5%, while age-standardized mortality and DALY rates decreased by 13% and 16%, respectively. Regionally, Australasia had the highest age-standardized incidence rate in 2021(19.7 per 100,000 population), whereas Central Latin America reported the lowest(0.57 per 100,000). At the national level, Canada showed the highest age-standardized incidence rate(26.8 per 100,000 population), followed by Greenland(24.6 per 100,000). Netherlands recorded the highest age-standardized death rate(2.21 per 100,000 population), with Singapore having the lowest (0.026 per 100,000).
Conclusion: The findings underscore the escalating global burden of IBD and highlight the need for targeted public health interventions. Effective strategies should focus on strengthening healthcare systems, enhancing specialized training, and promoting early detection and management of IBD to alleviate its impact on health outcomes and socioeconomic well-being globally.
PP-02-106
Study on the relationship between sleep disorders and autonomic function in patients with ulcerative colitis
Jiaming Zhou, Jian Wan and Kaichun Wu
Xijing Hospital Of Digestive Diseases, Air Forth Medical University, Xi’an, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the impact of sleep disorders on autonomic function, psychological symptoms and quality of life in ulcerative colitis (UC) patients.
Methods: UC patients from Xijing Hospital were prospectively enrolled from January 2024 to June 2024. The Pittsburgh Sleep Quality Index(PSQI) was used to assess sleep quality. The Generalized Anxiety Disorder-7(GAD-7), the Patient Health Questionnaire-9(PHQ-9), and the Inflammatory Bowel Disease Questionnaire(IBDQ) were used to evaluate psychological symptoms and quality of life. Heart rate variability was measured to assess autonomic function.
Results: 75 patients with active UC were included, with a mean age of 41.44 years, a higher proportion of males than females(60.0% vs 40.0%), and an average disease duration of 6.34 years. 50.7% of UC patients were in the mild active stage and 49.3% in the moderate to severe active stage. The prevalence of sleep disorders was 64.0%. Compared to UC patients without sleep disorders, those with sleep disorders had a higher proportion of comorbid anxiety(P=0.011) and depression(P<0.001), higher GAD-7 scores(P=0.009) and PHQ-9 scores(P<0.001), and lower IBDQ scores(P=0.008). HF, reflecting vagal nerve activity, was significantly lower in the UC group with sleep disorders(0.32±0.16 vs 0.41±0.16, P=0.027), while the LF/HF ratio was significantly higher(3.00±2.11 vs 2.02±1.76, P=0.024).
Conclusions: UC patients with sleep disorders have more severe anxiety and depression, poorer quality of life, and increased autonomic dysfunction, as evidenced by lower vagus nerve activity and higher sympathetic nerve activity. Exploring whether sleep disorders promote the development and progression of UC by affecting autonomic function is a direction for future research.
PP-02-107
Rifaximin Therapy Versus Low FODMAP Diet in Irritable Bowel Syndrome: A Randomised Controlled Trial
Wah Loong Chan1, Kee Huat Chuah1, Qing Yuan Loo1, Audrey Joe Chii Loh1, Wen Xuan Hian1, Xin Hui Khoo1, Sarala Panirsheeluam3, Hazreen Abdul Majid2 and Sanjiv Mahadeva1
1Gastroenterology and Hepatology Unit, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;2Department of Social Preventive Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;3Staff & Student Health Unit, University Malaya Medical Centre, Kuala Lumpur, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Dysbiosis contributes to the pathophysiology of irritable bowel syndrome (IBS), with antibiotic and dietary interventions potentially altering gut microbiota and alleviating symptoms. This study aims to compare the efficacy of Rifaximin versus a low FODMAP (fermentable oligosaccharides, disaccharides, monosaccharides, and polyols) diet (LFD) in IBS patients.
Materials and Methods: In this single-blinded, randomized controlled trial, IBS patients were assigned to either Rifaximin 400 mg TDS for 2 weeks or a dietitian-guided LFD for 4 weeks. The primary outcome was the proportion of patients with composite symptom improvement (abdominal pain/discomfort and bowel symptoms) at week 2 or 4. Secondary outcomes included improvements in IBS-symptom severity scale (IBS-SSS), global IBS symptoms, bloating, abdominal pain, stool consistency, and quality of life.
Results: A total of 84 patients (Rifaximin, n=43; LFD, n=41) were recruited (mean age: 49 years, 52.4% female, IBS-diarrhea 71.4%). The proportion of patients achieving composite symptom improvement did not differ significantly between the groups (67.4% vs 53.7%, p=0.196). The Rifaximin group showed significant improvement in bloating and stool frequency compared to the LFD group (93.0% vs 73.2%, p=0.015; 88.4% vs 68.3%, p=0.025). Both groups showed significant improvements in IBS-SSS and EQ-5D scores (p<0.001), with no statistically significant differences in the degree of change between the groups. There were no significant differences in improvement of abdominal pain and global symptoms. Sub-group analysis of IBS-D patients yielded similar results.
Conclusion: Both Rifaximin and LFD were effective in treating IBS, but Rifaximin was superior in improving bloating and stool frequency.
PP-02-109
Epidemiology and risk factors of gastrointestinal-symptoms and post-infectious bowel disorders as sequelae in COVID-19 patients
Gyung Mo Goo1, Kwang Woo Kim1, Ji Young Baek1, Bo Kyung Kim1, Hee Joon Jang1, Dong Kee Jang1, Su Hwan Kim1, Yong Jin Jung1, Ji Won Kim1, Byeong Gwan Kim2, Kook Lae Lee1 and Hyoun Woo Kang1
1Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul National University College of Medicine, Seoul, Republic of Korea;2Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Gastrointestinal manifestation of COVID-19 infection is now well known. However, there is few study about incidence of bowel disorders including post-infectious irritable bowel syndrome (PI-IBS), functional constipation, and functional diarrhea after long-term follow up. We investigate to evaluate the risk factors related with long-term post-COVID-19 (Coronavirus disease-2019) bowel disorders.
Methods: This single-center, prospective, observational study enrolled individuals infected with SARS-CoV-2 between November 2021 and February 2023. Baseline characteristics were collected at the time of infection, and follow-up surveys assessed bowel disorders based on ROME IV criteria.
Results: Among 149 initially asymptomatic subjects, 22 developed bowel disorders (7 PI-IBS, 5 functional constipation, 10 functional diarrhea) over a mean follow-up period of 15.5 months. The mean age at infection was 63.2 years, with males comprising 47.0% of the cohort. Alcohol consumption was significantly higher in the group with post-COVID-19 bowel disorders compared to controls (27% vs. 9%, p = 0.018). After adjusting for COVID-19 severity, multivariable logistic regression confirmed a significant association between alcohol consumption and post-COVID-19 bowel disorders. Subgroup analysis showed no significant differences in PI-IBS and functional constipation groups compared to controls, but the functional diarrhea group exhibited notable disparities in white blood cell count, absolute neutrophil count, and alcohol consumption rate (p = 0.044, p = 0.028, p = 0.020, respectively).
Conclusion: This study highlights a significant relationship between alcohol consumption and bowel disorders, particularly diarrhea, as long-term consequences SARS-CoV-2 infection. Further research is warranted to explore additional risk factors and elucidate the relationship between COVID-19 and gastrointestinal disorders.
PP-02-110
Symptomatology aspects differentiating irritable bowel syndrome (IBS) and organic diseases: age and sex effect
Amal Arifi Hidayat1,4, Hoda M Malaty3,2, Langgeng Agung Waskito1,4, Titong Sugihartono2, Pangestu Adi2, Husin Thamrin2, Amie Vidyani2 and Muhammad Miftahussurur2,4
1Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;2Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;3Department of Medicine, Baylor College of Medicine, Houston, United States;4Helicobacter pylori and Microbiota Study Group, Institute Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: To investigate the clinical features that distinguish between irritable bowel syndrome (IBS) and organic diseases among patients with typical IBS symptoms.
Materials and Methods: Patients fulfilling the Rome IV IBS criteria were assessed for their demographic data, detailed bowel symptoms, and alarm features. All patients underwent routine laboratory tests and a colonoscopy investigation. Based on the colonoscopy findings, patients were assigned into two groups: IBS or organic disease group. Univariate and multivariate analysis were performed to compare clinical features between the two groups.
Results: Of the 83 patients recruited, 43 (51.8%) were classified into IBS group, and 40 (48.2%) were into organic disorders: 30 (75%) IBD, 2 (5%) malignancy, 6 (15%) polyps, and 2 (5%) diverticulitis. Patients aged ≥40 years demonstrated a higher prevalence of organic diseases, irrespective of sex, with a statistically significant difference (36.6% vs. 59.5%, p<0.05). Alarm features were highly prevalent in both groups and failed to demonstrate significant differences (53.5% vs. 47.5%, p>0.05). Among patients with IBS symptoms, a higher frequency of abdominal pain (OR=2.24, 95%CI 1.02-4.89, p<0.05) and pain that does not improve after defecation (OR=21.01, 95%CI 4.83-91.33, p<0.01) were independently correlated with the presence of organic diseases.
Conclusion: Half of the patients presenting with typical IBS symptoms had organic diseases. Colonoscopies should be considered for all patients aged ≥40 years with IBS symptoms, regardless of sex or the presence of alarm features. More frequent abdominal pain and pain that does not improve after defecation are predictors of the presence of organic diseases.
PP-02-111
Prevalence of NAFLD and MAFLD in patients with Irritable Bowel Syndrome
Musab Khalil
Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Both NAFLD and IBS may share common risk factors. Data are sparse regarding the frequency of Metabolic Dysfunction associated Fatty Liver Disease (MAFLD) among Irritable Bowel Syndrome (IBS) patients.
Methods: We have conducted a cross-sectional comparative study at the outpatient department of SRNGIH to find out the frequency of Non-Alcoholic Fatty Liver Disease (NAFLD) and Metabolic dysfunction Fatty Liver Disease (MAFLD) among patients with Irritable Bowel Syndrome (IBS).
Results: Out of 219 patients,121 patients had IBS. Irritable Bowel Syndrome - Diarrhea Predominant (IBS-D) was the most prominent type (55%). One-third (n=39) of the IBS (32.23%) patients had NAFLD. One-tenth [n=12] had MAFLD. Significantly younger patients had NAFLD among IBS patients compared to non-IBS patients [p=0.023]. Non-IBS patients had significantly more Low-Density Lipoprotein (LDL) compared to IBS patients (p=0.040). Serum triglycerides were higher among IBS patients having NAFLD compared to non-IBS patients having NAFLD (p=0.022). Serum Alanine Aminotransferase (S. ALT) was significantly higher in IBS patients with NAFLD than in IBS patients without NAFLD (p=0.006). Body Mass Index (BMI) was significantly higher in IBS patients with MAFLD than in IBS patients without MAFLD. Total and LDL cholesterol were significantly higher in IBS with MAFLD patients than in IBS without MAFLD.
Conclusion: One-third of Irritable Bowel Syndrome (IBS) patients had Nonalcoholic Fatty Liver Disease (NAFLD). Metabolic dysfunction associated with Fatty Liver Disease (MAFLD) was less prevalent among patients with IBS.
PP-02-112
Study on the Therapeutic Effects and Mechanisms of Gintonin in Irritable Bowel Syndrome
Na Ri Choi1,2, Seok Jae Ko3,4, Joo Hyun Nam5,6, Woo-Gyun Choi1, Jong Hwan Lee7, Seung-Yeol Nah8, Jae Woo Park3,4 and Byungjoo Kim1
1Department of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, South Korea;2Department of Korean Medical Science, Pusan National University School of Korean Medicine, Yangsan, South Korea;3Department of Clinical Korean Medicine, Graduate School of Kyung Hee University, Seoul, South Korea;4Department of Gastroenterology, College of Korean Medicine, Kyung Hee University, Seoul, South Korea;5Department of Physiology, Dongguk University College of Medicine, Kyungju, South Korea;6Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang, South Korea;7Department of Biomedical Engineering, Dong-Eui University College of Engineering, Busan, South Korea;8Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Irritable bowel syndrome (IBS) is a gastrointestinal disease accompanied by changes in bowel habits without any specific cause. Gintonin is a newly isolated glycoprotein from ginseng that is a lysophosphatidic acid (LPA) receptor ligand.
Materials and Methods: To investigate the efficacy and mechanisms of action of gintonin in IBS, we developed a zymosan-induced IBS murine model. In addition, electrophysiological experiments were conducted to confirm the relevance of various ion channels.
Results: In mice, gintonin restored colon length and weight to normal and decreased stool scores, whilst food intake remained constant. Colon mucosal thickness and inflammation-related tumor necrosis factor-α levels were decreased by gintonin, along with a reduction in pain-related behaviors. In addition, the fecal microbiota from gintonin-treated mice had relatively more Lactobacillaceae and Lachnospiraceae and less Bacteroidaceae than microbiota from the control mice. Moreover, gintonin inhibited transient receptor potential vanilloid (TRPV) 1, TRPV4, and voltage-gated Na+ 1.5 channels associated with visceral hypersensitivity.
Conclusion: These results suggest that gintonin may be one of the effective components in the treatment of IBS.
PP-02-113
Food Avoidance, COVID-19 Infection and Post-Infectious Functional Gastrointestinal Disorders: Is There A Link?
1Faculty of Health Sciences, National University of Malaysia, Kuala Lumpur, Malaysia;2Department of Medicine, Hospital Canselor Tuanku Mukhriz, National University Hospital Malaysia, Cheras, Malaysia;3Department of Gastroenterology, National University of Singapore, Lower Kent Ridge Rd, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: COVID-19 increases the risk of Post-infectious Functional Gastrointestinal Disorders (PI-FGID). This case-control study identified the frequency of FGID, and determine its impact on the quality of life, psychosocial status and eating habits of Malaysian patients.
Methods: A total of 227 subjects, comprising of 116 healthy controls and 111 COVID-19 cases completed the study using validated and translated questionnaires. Chi-square test was performed to determine relationship between categorial variables.
Results: A total of 3.1% cases and 2.2% controls developed PI-FGID over 1 month post-COVID-19 infection with an odds ratio of 1.49 with no significant differences between groups (p>0.05). There was a significant relationship between FGID and quality of life with regards to level of pain/discomfort (p=0.037) and level of anxiety/depression (p=0.000) among cases. Psychological status was significantly correlated with FGID (p=0.027) particularly those who have experienced abuse. A total of 14.1% (n=227) subjects reported improvement in gastrointestinal symptoms following food avoidance whereby fructan was the most common FODMAP that was avoided.
Conclusions: COVID-19 increases the risk of PI-FGID impacting quality of life. Patients with stressful life events have an increased risk and may avoid trigger foods high in FODMAPs.
PP-02-114
A case report: Crohn’s Disease Diagnosed After Rectal Mucosa-associated Lymphoid Tissue (MALT) Lymphoma
Hua Yen Ling, H Lin and Jacqueline Zhiling Yang
Tan Tock Seng Hospital, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: We report a case of Crohn’s disease (CD) diagnosed after MALT treatment and Cytomegalovirus (CMV) disease.
Case Description: A previously well 60-year-old Malay male presented with 2 weeks of haematochezia. Colonoscopy showed circumferential mucosal irregularity of lower rectum. Biopsy confirmed rectal MALT. He underwent 15 cycles of definitive radiotherapy and computed topography 6 months post treatment showed stable changes. However, he still had intermittent mild haematochezia despite being in disease remission. Flexible sigmoidoscopy that was done reported radiation proctitis without biopsy taken. He was prescribed one week of hydrocortisone enema.
A trip to rural village in Indonesia worsened his haematochezia necessitating hospitalisation. Stool infective screen was negative. Repeat sigmoidoscopy showed multiple patchy deep, cratered ulcers with inflamed mucosa throughout. Histology showed dense active chronic inflammation and ulcerations with inclusion bodies. He completed 3 weeks of Cytomegalovirus treatment.
Repeat colonoscopy was done to document CMV resolution although haematochezia resolved. It showed persistent scattered ulcers, pseudopolyps with loss of vascular pattern from rectum to distal ascending colon. Histology did not suggest any residual lymphoma; skip segment and patchy inflammation were more suggestive of Crohn’s disease. He was started on mesalazine enema with improvement of symptoms.
Discussion: The association between CD and MALT lymphoma remains controversial. Inflammatory bowel disease (IBD) associated lymphoma was proposed to be related to treatment (thiopurine/anti-TNF), Epstein-Barr virus and chronic inflammation. However in our case, lymphoma was diagnosed before CD and it is difficult to determine causal effect as no whole colon biopsies were taken during index colonoscopy.
PP-02-115
Abnormalities in anorectal physiology amongst constipated individuals – data from a Singaporean centre
Valerie Yeap and Andrew Ong Ming Liang
Singhealth / Singapore General Hospital, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: Chronic constipation is a common problem in gastroenterology, yet it remains a challenge to treat. Anorectal physiology testing aids in the evaluation and management of constipation, and the it provides enables treatment plans to be tailored accordingly.
Our aim was to evaluate the abnormalities in anorectal function amongst patients with chronic constipation, to achieve better understanding of the pathophysiological mechanisms underlying their symptoms. These anorectal manometry (ARM) findings were then compared against the various forms of treatment patients received.
Materials and methods: This was a retrospective analysis of all patients who underwent ARM testing for constipation symptoms between January 2021 and December 2023 in Singapore General Hospital. The ARM parameters assessed were anal sphincter pressures ( rest and squeeze), recto-anal coordination during simulated defecation, rectal sensation and recto-anal reflex activity. Electronic clinic notes and prescription charts were reviewed to evaluate how patients’ symptoms were simultaneously managed.
Results: A total of 223 patients underwent ARM for evaluation of constipation, of which 62% (N=138) were female. The median age was 59. 80% were of Chinese ethnicity, the remaining were Malay (6%), Indian (6%) and of other ethnicities (8%). Dyssynergia was the most common finding (70%,N=157), followed by rectal hypersensitivity (57%,N=128). A combination of rectal hypersensitivity and dyssynergia was seen in 39%(N=87) of patients.
The most common treatment undertaken was laxatives (92%,N=204), followed by biofeedback therapy (60%,N=134). Prucalopride was prescribed in (21%,N=47) and neuromodulators only in (10%,N=23).
Conclusion: Rectal hypersensitivity and dyssynergia are common and often co-exist amongst patients with chronic constipation. The treatment of their symptoms should therefore be tailored accordingly. More can be done to address rectal hypersensitivity amongst constipated individuals.
PP-02-116
Immune cells and irritable bowel syndrome: A bidirectional Mendelian randomization study of their causal relationship
Wan Zhou1 and Pan Xu2
1Department of Gastroenterology, University-Town Hospital of Chongqing Medical University, Chongqing, China;2Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: The causal relationship between immunophenotype and irritable bowel syndrome (IBS) remains unclear. We conducted a bidirectional Mendelian randomization (MR) study using genome-wide association data (GWAS) to evaluate this association.
Method: Leveraging GWAS data, we explored 731 immunophenotypes' causal interplay with IBS. The inverse variance weighting (IVW) method was employed as the primary analytical approach. The MR-PRESSO outlier test, Cochran's Q heterogeneity test, and MR Egger intercept test were utilized for sensitivity analyses.
Results: After false discovery rate (FDR) correction, four immunophenotypes were found to be linked with an increased risk of IBS: CD27 on CD24+ CD27+ B-cell (odds ratio [OR]=1.04; 95% confidence interval [CI], 1.02–1.06; p < 0.001); CD27 on IgD- CD38- B-cell (OR=1.04; 95% CI, 1.02–1.07; p < 0.001); CD27 on IgD- CD38dim B-cell (OR=1.03; 95% CI, 1.01–1.06; p < 0.001); and CD27 on sw mem B-cell (OR=1.03; 95% CI, 1.01–1.05; p < 0.001). One immunophenotype negatively correlated with the risk of IBS: IgD- CD27- %B-cell (OR=0.93; 95% CI, 0.90–0.97; p < 0.001). Statistically significant associations were not found among the 731 immunophenotypes and IBS in the reverse MR analysis.
Conclusions: Four immunophenotypes (CD27 on CD24+ CD27+, CD27 on IgD- CD38-, CD27 on IgD- CD38dim, CD27 on sw mem B-cell) are risk factors for IBS, while one immunophenotype (IgD- CD27- %B-cell) is a protective factor against IBS, and they all belong to B cells. This finding further elucidates the role of immune factors in the risk of IBS and provides a basis for subsequent clinical investigations.
PP-02-117
Polyposis Syndrome - Familial Adenomatous Polyposis with rectal adenocarcinoma presenting as hematochezia: A case-report
John Derek Clutario, Marc Julius Navarro and Jose Luis Matthias Sollano
Makati Medical Center, Pasig, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Familial Adenomatous Polyposis (FAP) is a hereditary condition characterized by the development of numerous colorectal adenomatous polyps, often in the hundreds to thousands. This autosomal dominant disorder is caused by mutations in the APC (Adenomatous Polyposis Coli) gene. Without prophylactic treatment, nearly all individuals with FAP will develop colorectal cancer by the age of 40.
Case description: In this report, we present a case of a forty two year old female who has been having hematochezia for a year. She has only been treated as a case of grade 4 internal hemorrhoids however was not worked up further due to financial constraints. CT scan done, it revealed a semi-circumferential 6.4cm mass in the mid to low rectum hence a colonoscopy was done. Findings were multiple (estimated to be in the thousands) polypoid lesions spanning the entire colonic tract. Biopsies in the colon revealed tubular and tubulovillous adenoma and unfortunately biopsy from the rectum revealed rectal adenocarcinoma. On retrospective history taking, the patient did not have any known relatives with the same symptoms but she has already lost touch with majority of her relatives. Specimens will be sent abroad for complete genetic testing and the patient is now being prepared for total proctocolectomy.
Discussion: This case underscores the importance of vigilant surveillance and timely intervention in individuals with FAP, as well as the clinical implications of rectal bleeding in this high-risk population. Rectal adenocarcinoma, a common complication of FAP, can present with various symptoms, among which hematochezia—rectal bleeding.
PP-02-118
Diverticular Bleeding: A Case Study in Diagnostic and Therapeutic Approach
Albert William Hotomo1, Ahmad Nur Aulia2, Andhiky Raymonanda Madangsai2 and Muhammad Firhat Idrus2
1Departement of Internal Medicine, FKKH UNDANA, Kupang, Indonesia;2Pusat Endoskopi Saluran Cerna, RSUPN Dr. Cipto Mangunkusumo/FKUI, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Diverticular bleeding, characterized by painless hematochezia, is a prevalent gastrointestinal complication with a significant age-related incidence. Nonsteroidal anti-inflammatory drugs, aspirin, and antiplatelet agents are established risk factors. Hypertension has been associated with vascular injury, which may exacerbate the condition.
Case Description: An 84-year-old woman presented with recurrent hematochezia 4 hours prior at around 50 mL volume. Her medical history included hypertension and routine NSAID use. She denied wasting symptoms or a history of malignancy. Physical examination revealed pale conjunctiva, tenderness of the left lower abdominal region, and digital rectal examination showed a reddish-brown stool and blood. Blood test evaluation demonstrated anemia, acute renal failure due to blood loss, and normal coagulation test. The 1st Colonoscopy showed minimal multiple diverticular bleeding at the descending colon. Hemoclip or argon plasma coagulation has been planned if there is any active bleeding from the diverticle. Omeprazole, tranexamic acid, and sucralfate were administered to alleviate minimal active bleeding from the diverticula, along with a high-fiber diet. A subsequent colonoscopy showed no active bleeding.
Discussion: Diverticular bleeding is typically managed with a combination of medical and endoscopic treatments, with surgery reserved for severe cases. While other available pharmacologic treatments like vasopressin can reduce blood flow to the intestines, the effectiveness of tranexamic acid for lower gastrointestinal bleeding is still under investigation. Preventing diverticulosis through a high-fiber diet, adequate hydration, and regular exercise can lower the risk of bleeding.
PP-02-119
A Case of Disseminated Tuberculosis presenting as Massive Gastrointestinal Bleeding in an Immunocompetent Patient
Natasha Bernadine Mapa and Carissa Marin
Makati Medical Center, Makati City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal tuberculosis (GITB) is an uncommon form of extrapulmonary tuberculosis that poses a diagnostic challenge due to its non-specific clinical presentation. Among its varied manifestations, gastrointestinal bleeding as an initial presentation is exceedingly rare. This case report aims to highlight the diagnostic difficulties, treatment modalities, and clinical outcomes in a patient with GITB presenting as gastrointestinal bleeding.
Case Description: This is a case of a 24/M, no known comorbidities, immunocompetent host, no maintenance medications who came in for sudden onset of hematochezia with progressive drop in hemoglobin levels. Work up revealed a circumferential wall thickening involving the ileocecal region. Bleeding from mass was difficult to control despite medications and colonoscopy attempts hence patient underwent exploratory Laparotomy, right segmental colectomy. Histopathology results revealed caseating chronic granulomatous inflammation and lymph nodes showing caseating granulomas. Patient was then started on anti-Kochs treatment with note of improvement of symptoms post treatment.
Discussion: Gastrointestinal tuberculosis (GITB) which can mimic various other gastrointestinal disorders makes the diagnosis challenging, often leading to delayed or missed identification. Gastrointestinal bleeding as an initial presentation is an unusual but documented occurrence. The treatment of gastrointestinal tuberculosis presenting as bleeding involves a comprehensive approach that typically combines anti-tubercular therapy (ATT) with surgical intervention. A high index of suspicion accompanied by promptly intervention are key to improving patient care.
PP-02-120
Small Intestinal Burkitt's Lymphoma presenting as an Acute Abdomen secondary to Ileo-Colic Intussusception
Christine Velasquez, Beatrice Delynn Go and Gerardo Pedregosa
Makati Medical Center, Makati, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Primary gastrointestinal lymphomas are rare, comprising 1–4% of gastrointestinal malignancies, with Burkitt’s lymphoma accounting for 0.3–1.3% of non-Hodgkin’s lymphomas. Typically affecting the ileum in children and adolescents, Burkitt’s lymphoma in adults can cause ileocolic intussusception. This report presents a rare case of ileocolic intussusception caused by small intestinal Burkitt’s lymphoma.
Case description: A 28-year-old male presented with progressive colicky pain in the right lower quadrant for 4 days, accompanied by nausea and bloatedness. Medical history was unremarkable. Physical examination revealed a distended abdomen, hypoactive bowel sounds, tympanitic percussion, and direct and rebound tenderness in the right lower quadrant with guarding. Laboratory tests revealed leukocytosis, and CT scan identified a 3.5x4.2x3.6 cm mass at the ileocecal junction, indicating ileocolic intussusception The patient underwent a right hemicolectomy and ileo-transverse colostomy with end-to-side anastomosis. Intraoperative findings revealed a 5 cm intussuscepted ileum in the cecum, a 4x4x3 cm ileocecal valve lead point, and a 1.5 cm tumor. Biopsy was consistent with Burkitt’s lymphoma. The patient received chemotherapy with rituximab, etoposide, doxorubicin, vincristine, prednisone, cyclophosphamide, and filgrastim for 6 cycles. Follow-up CT scans and colonoscopy showed no new masses and normal colonic mucosa.
Discussion: Burkitt’s lymphoma, linked to previous malabsorption syndromes, IBD, and immunosuppression, presents with nonspecific symptoms, such as abdominal pain. Diagnosis involves CT scans and laparotomy. Biopsy shows CD20 positivity and atypical lymphoid proliferation. Treatment includes surgical resection and chemotherapy. Prognosis varies by age and disease site. The survival rate is approximately 60%. More research on adult cases is recommended.
PP-02-121
Controlling nutritional status score as predictive marker in completing adjuvant capecitabine for biliary tract cancer
Sung Hoon Chang, Jun Yeol Kim, Yong Soo Song, Tae Seung Lee, Jin Ho Choi, Woo Hyun Paik, Sang Hyub Lee, Ji Kon Ryu and In Rae Cho
Seoul National University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Adjuvant capecitabine has demonstrated survival benefit for patients with resected biliary tract cancer (BTC). However, about one-third of clinical trial population discontinued treatment due to toxicity. Previous studies have found that nutritional indices such as the Geriatric Nutritional Risk Index (GNRI) and the Controlling Nutritional Status (CONUT) score are related to chemotherapy tolerance. This study aims to assess whether nutritional indices can predict completion of adjuvant capecitabine and prognosis.
Materials and Methods: We retrospectively investigated 61 BTC patients treated with adjuvant capecitabine from June 2019 to January 2023. Patients were categorized into two groups: those who completed adjuvant capecitabine and did not. Pre-treatment nutritional indices including GNRI, CONUT score, platelet-to-lymphocyte ratio, and neutrophil-to-lymphocyte ratio were analyzed and compared between the groups. Overall survival (OS), recurrence-free survival (RFS) and treatment-related adverse events (TRAEs) were also investigated.
Results: Of 61 patients, 44 (72.1%) completed adjuvant capecitabine. Among the various nutritional indices, the CONUT score was significantly different between completion and incompletion groups (2 vs. 3; p=0.033). CONUT score ≤2 was associated with higher completion rates of adjuvant capecitabine (83.9 % vs. 55.6 %; p=0.038) and higher relative dose intensity (90% vs. 80%, p=0.044). Patients who completed adjuvant chemotherapy experienced fewer TRAEs (61.0% vs 94.1%; p=0.027) and tended to show better 3-year OS (37% vs. 18%; p=0.089) and RFS rates (22% vs. 18%; p=0.51).
Conclusion: The CONUT score is helpful for predicting the completion of adjuvant capecitabine. It can aid in selecting appropriate patients for adjuvant chemotherapy and predicting prognosis.
PP-02-122
New chemical drugs MTM16 relieves cholesterol stones by regulating cholesterol metabolism
博士 Yirong Chi
Department Of Gastroenterology And Hepatology, Tianjin Medical University General Hospital, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Gallbladder stones are a common disease worldwide, characterized by a homeostatic imbalance of cholesterol, bile acids, and other components of bile. The novel chemical CPLT was found to have lipid-lowering effects in preliminary experiments, so we hypothesized that it might have a mitigating effect on gallbladder cholesterol stone formation. The aim of this study was to investigate the therapeutic effects and potential mechanisms of CPLT on cholesterol stones.
Methods: C57BL/6J mice were randomly divided into 3 groups: the NC group (normal diet, equal volume of 1% DMSO), the LD group (stone-forming diet group, equal volume of 1% DMSO), and the CP group (LD diet, 30 mg/kg/d CPLT), which were fed and gavaged for 4 weeks. Lipid profiles in gallbladder bile were measured and cholesterol saturation index (CSI) was calculated. Metabolomic analysis of bile acid species in liver and feces was performed. Serum was taken for analysis of liver function and lipids. Gallbladder and liver tissues were taken for HE staining. The expression of cholesterol metabolism and transport genes in liver and ileum was detected.
Results: CPLT reduced the gallstone score as well as CSI in mice, and led to a decrease in cholesterol concentration in gallbladder and lipid concentration in serum.CPLT improved hepatic fat vacuoles, and the thickness and structure of the gallbladder wall, etc. in the CP group improved compared with that in the LD group. The real-time PCR and Western blot assays revealed that ABCG5,ABCG8 and upstream LXRα were down-regulated in the liver by CPLT.
PP-02-123
The bleeding pancreas: case report of pancreatic carcinoma with contained pseudoaneurysm rupture status post embolisation
Kunhan Chiam
Tan Tock Seng Hospital, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Pancreatic pseudoaneurysm is a rare vascular complication of pancreatitis resulting from erosion of the peripancreatic artery. It usually involves the splenic artery, gastroduodenal, pancreaticoduodenal, superior mesenteric artery. Bleeding can occur in the pseudocyst itself and even into surrounding organs. Management is embolization or surgical intervention. We report a patient with pancreatic carcinoma with a pseudoaneurysm.
Case Description and Discussion: A 65 year old female presented with 3 months of epigastric pain with an epigastric mass on examination. Computed tomography scan showed a large 9.8cm x 8.2cm head of pancreas cystic-solid mass. Within the mass there was a 1.9cm lobulated area of focus with arterial phase enhancement and persistent hyperdensity in subsequent phases consistent with a pseudoaneurysm. Another 1.6cm segment 6 hepatic lesion seen likely metastasis. She underwent a liver biopsy of the S6 hepatic lesion which showed poorly differentiated carcinoma with neuroendocrine differentiation (positive for CK7, PAX8, Synaptophysin, negative for WT1, Ki67 stains 70-80% of the tumour cells. She was started on chemotherapy with Etoposide and Carboplatin.
Investigation: Hb 11.2 g/dL, Bil 7umol/L, AST 17 ALT 11 ALP 80 CA19-9 363 U/ml (Insert initial CT here) 2 months later, she had worsening abdominal pain with per-rectal bleeding and repeat CT showed enlargement of the pseudoaneurysm to 4.7cm with contained rupture. Hb 7.1 g/dL (Insert CT here)
She underwent radiologically guided embolization of the pseudoaneurysm (Insert below and after images here with description)
Patient’s symptoms improved and she was continue on chemotherapy and planned for repeat CT two weeks thereafter.
PP-02-124
Hemobilia from an intraductal papillary neoplasm of the bile duct presenting as recurrent melena
Jemimah Andrea Fajardo and Edgardo Bondoc and Evan Ong
St. Luke's Medical Center Quezon City, Quezon, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Hemobilia, a bleeding event from or into the biliary tract, still remains to be an uncommon cause of gastrointestinal bleeding worldwide. Its clinical presentation and etiology may vary which is often a challenge as it can lead to potential lethal sequelae if not properly recognized and treated. Hence, a systematic approach is prudent to locate and possibly rule out rare malignancies.
A 70-yo-Filipino-female, known hypertensive-diabetic, cervical, colon carcinoma in remission complained of 4-month history of recurrent melena underwent Esophagogastroduodenoscopy(EGD), Magnetic-resonance-cholangiopancreatography(MRCP), Enteroscopy two-months prior revealed chronic gastritis, gastric ulcers FCIII.
Melena recurred hence repeat EGD, push enteroscopy revealed Hemobilia. To control bleeding, underwent Celio-Mesenteric Angiogram subsequent Particle Embolization of Distal Right Hepatic Arteries, Distal Middle Hepatic Artery.
Dynamic CT scan of the liver and abdomen clinched the location it revealed intraluminal heterogenous foci, hyperdensities in the dilated common bile duct, intrahepatic bile ducts, nodular enhancing focus. As to exhaust the investigation, a Gastrointestinal bleed scintigraphy and direct visual Cholangioscopy was done to go beyond across the ducts in an odyssey to reveal a lobulated polypoid lesion, villous surface at the main right intrahepatic duct. Biopsy revealed intraductal papillary neoplasm, low grade intraepithelial neoplasia concluded the quest.
Referred to Surgery, final biopsy signed out as Intraductal Papillary Mucinous Neoplasm Of The Bile Duct(IPNB), focal high grade dysplasia. Patient was discharged, given palliative care.
IPNB remains to be a rare cause of UGIB.
A prompt diagnosis through a systematic approach through various advanced diagnostic procedures is necessary in preventing disease progression, and mortality.
PP-02-126
Effect of diabetes and insulin resistance on treatment outcomes in patients with pancreatic cancer chemotherapy
NAM HEE Kim and Hong Joo Kim
Sungkyunkwan University Kangbuk Samsung Hospital, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We investigated whether diabetes mellitus (DM) and insulin resistance have any association with survival of patients with pancreatic cancer (PC) undergoing chemotherapy.
Materials and methods: From March 2013 to March 2023, 265 patients who were pathologically confirmed to have PC at our hospital, received palliative, neoadjuvant, or adjuvant chemotherapy, and had a follow-up period of more than 6 months were enrolled in the study.
Results: The overall survival of DM patients was significantly shorter than that of patients without DM (median survival for DM and non-DM patients was 7 and 12 months, respectively, p=0.038 by log-rank comparison). The overall survival of high fasting insulin group (defined as fasting insulin ≥ 10 uIU/ml) was significantly shorter than that of low fasting insulin group (median survival for high fasting insulin and low fasting insulin group was 5 and 17 months, respectively, p <0.01 by log-rank comparison). The overall survival of high HOMA-IR group (defined as HOMA-IR >4%) was significantly shorter than that of low HOMA-IR group (median survival for high HOMA-IR and low HOMA-IR group was 8 and 26 months, respectively, p <0.01 by log-rank comparison). Independent prognostic factors in pancreatic cancer patients receiving chemotherapy according to Cox regression analyses were high HOMA-IR group, NCCN stage, and initial RECIST.
Conclusions: In pancreatic cancer patients receiving chemotherapy, the presence of diabetes and insulin resistance appear to be one of the factors that determine treatment outcomes.
PP-02-127
Neutrophil to Lymphocyte Ratio Predicts Infections in Acute Pancreatitis
Gauri Kumbhar, Reuben Thomas Kurien and Sudipta Dhar Chowdhury
Christian Medical College, Vellore, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Differentiating infections from sterile inflammation is crucial in early Acute Pancreatitis (AP) management. This study aimed to analyse the profile, the impact of infections in AP and to assess the capability of the Neutrophil-to-Lymphocyte Ratio (NLR) to discriminate between sterile inflammation and infections in the first week of illness.
Materials and Methods: Patients with AP developing a fever were included. Infections were categorized as infected pancreatic necrosis(IPN) and extra-pancreatic infections(EPI). Microbiological profiles and serious adverse events (SAE-in hospital mortality or discharge in critical state) were analysed. The ability of NLR and procalcitonin to discriminate infection from sterile inflammation in the first week was assessed.
Results: Of 505 patients with AP, 150 developed fever. 48 (32%) had sterile inflammation, while 102 (68%)had infections. Within infections, 44 had IPN, 68 had EPI, and 10 had both. Of 54 patients with culture-positive infections, 36(66.6%) had infections by multidrug-resistant (MDR) organisms. Fungal isolates were identified in 5 patients. Patients with infections had higher SAE incidence (21.6% vs. 4.2%,p = 0.007) than those with sterile inflammation. The SAE incidence was higher among patients with MDR infections than those without MDR (37.5% vs. 9.3%,p=0.006). Most patients (n = 98, 65.3%) developed fever during the first week of illness. NLR demonstrated good accuracy in discriminating infections from sterile inflammation in the first week (AUC 0.70,p= 0.001), outperforming procalcitonin (AUC 0.54,p = 0.58).
PP-02-128
Immune phenotype by AI-powered spatial analysis of tumor-infiltrating lymphocytes predicts prognosis in resected pancreatic cancer
Jong Kyun Lee1, Hyemin Kim1, Young Hoon Choi1, Yoo Joo Lim2, Kyu Taek Lee1, Kwang Hyuck Lee1 and Joo Kyung Park1
1Department Of Medicine, Samsung Medical Center, Sungkyunkwan University School Of Medicine, Seoul, South Korea;2Lunit Inc, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Tumor-infiltrating lymphocyte(TIL) have been implicated as prognostic biomarkers across various malignancies. However, their prognostic relevance in resected pancreatic ductal adenocarcinoma(PDAC) remains elusive. This study evaluates the utility of artificial intelligence(AI)-powered spatial mapping of TIL density in PDAC and its correlation with clinical outcomes.
Materials and Methods: A total of 304 PDAC patients who underwent upfront surgery with curative intention enrolled in this study, and their R0 resected specimens of whole slide images stained with hematoxylin and eosin(H&E) were analyzed. We utilized Lunit SCOPE IO, an AI-powered WSI analyzer, for spatial TIL quantification, segmentation of tumor and stroma, and immune phenotype(IP) classification as immune inflamed phenotype(IIP), immune excluded phenotype(IEP), or immune desert phenotype(IDP).
Results: The median intratumoral TIL(iTIL) density and stromal TIL (sTIL) density were 100.64/mm2 (Interquartile range[IQR], 53.25–121.39) and 734.88/mm2 ((IQR 443.10–911.16), respectively. The TILs in the tumor microenvironment were predominantly concentrated in the stroma, and both iTIL and sTIL were significantly higher in IIP compared to IEP and IDP (P< 0.001). IIP was favorably related to overall survival(OS) and recurrence-free survival(RFS), and exceptionally high iTIL density was associated with longer OS (P=0.004) and RFS (P=0.021). This prognostic significance persisted in multivariable analysis, including other clinicopathologic factors.
Conclusion: Notably, a higher density of iTILs was associated with improved prognostic outcomes. AI has markedly condensed the labor-intensive process of TIL assessment, potentially rendering TIL more feasible and practical in clinical application. More importantly, immunophenotype can be one of the most important biomarkers to predict OS and RFS in resected PDACs.
PP-02-129
Triceps Skinfold Thickness and Serum Albumin Changes in Pancreatic Cancer Chemotherapy: Sex-Based Prognostic Insights
Jong Kyun Lee, Young Hoon Choi, Kyu Taek Lee, Kwang Hyuck Lee and Joo Kyung Park
Department Of Medicine, Samsung Medical Center, Sungkyunkwan University School Of Medicine, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Cachexia is prevalent in pancreatic cancer patients. We explored the association between triceps skinfold thickness(TSF), indicative of body fat, and serum albumin levels, reflecting nutritional status, with overall survival(OS) in pancreatic cancer patients.
Materials and Methods: A prospective cohort of 353 pancreatic cancer patients receiving chemotherapy was analyzed. TSF and serum albumin were measured at baseline and every three weeks during follow-up. We performed a landmark analysis of OS six months after the diagnosis of pancreatic cancer and assessed the factors influencing OS. Sex-specific survival analysis was conducted, culminating in the construction of nomograms for risk stratification.
Results: Two hundred ninety-two patients survived over six months, with a median follow-up of 40.9 months. Changes in TSF, albumin, and protein levels over six months and initial albumin levels were independently associated with OS. Cancer stage, response to chemotherapy, and six-month serum CA19-9 levels were also independent factors for OS. Sex-stratified analysis revealed sex-specific prognostic factors: TSF(mm) changes over six months≤ 0 were associated with reduced OS (hazard ratio[HR], 1.84; 95% confidence interval[CI], 1.14–2.95) in males; initial albumin levels≤ 3.5g/dl (HR, 5.73; 95% CI, 2.48–13.21) and albumin changes over six months≤ -0.7g/dl (HR, 3.03; 95% CI, 1.51–6.07) were associated with reduced OS in females. Patients were categorized into low-, medium-, and high-risk groups per sex using nomogram scores, with increased risk levels linked to higher mortality risk.
Conclusion: This study highlights the significant association of TSF and serum albumin levels with OS in pancreatic cancer patients undergoing chemotherapy, differing by sex.
PP-02-130
Antibiogram and Bacterial Profile Susceptibility in Patients with Suspected Cholangitis Undergoing Biliary Drainage
Abhay Mahajan, C Ganesh Pai, Ganesh Bhat, Shiran Shetty, Balaji Musunuri and Athish Shetty
KMC, Manipal, Udupi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: The present study aims to identify the most commonly grown bacteria, their antibiotic susceptibility, and the risk factors for bacterial colonization in bile collected during biliary intervention in patients with different causes of biliary obstruction.
Methods: This prospective study was conducted at tertiary care of coastal Karnataka. Patients aged>18 and with suspected cholangitis undergoing ERCP and percutaneous drainage were included in the study. Bile specimens were collected and transported to the microbiology lab in blood culture bottles using an aerobic transport system.
Results: A total of 50 patients were enrolled in the study with a mean age of 48.39±13.5, with most being male individuals [n, 42 (84%)]. Of 50, 38 were culture positive. The most frequently encountered organisms were Gram-negative bacteria, including Escherichia coli [n, 12 (28.2%)], followed by Klebsiella [n,7 (17.3%)]. Among the antibiotics, amoxicillin-clavulinic acid [n,7 (18.4%)] and ceftriaxone [n,5 (13.1)] were found to be more resistant. No significant correlations were found between the type of bacterial colonization and antibiotic sensitivity to a type of obstruction prior to biliary interventions. However, a history of prior antibiotic use was found to be a significant factor associated with the risk of antibiotic resistance.
Conclusions: The present study recommends cautious antibiotic selection in cholangitis management, given the predominance of Gram-negative bacteria such as Escherichia coli and Klebsiella in bile cultures and observed resistance to amoxicillin-clavulanic acid and ceftriaxone. Further, the present study recommends developing local antibiotic treatment regimens concerning their antibiotic susceptibility.
PP-02-131
Shedding Light on a Rare Encounter: Case Report of Pancreatic Neuroendocrine Tumor in the Philippines
Natasha Bernadine Mapa and Roel Galang
Makati Medical Center, Makati City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Pancreatic neuroendocrine tumors (pNETs) represent a rare subset of neoplasms arising from the endocrine cells of the pancreas. These tumors are characterized by their unique biological behavior, diverse clinical manifestations and potential for hormone production.
Case description: This is a case of 39/M, no known comorbidities who came in for hematuria and back pain. Work up revealed a pancreatic mass with multiple liver lesions to consider metastasis. Tumor markers requested were unremarkable. Full PET CT showed a DOTATATE-avid mass in the pancreas with pituitary, neck, peripancreatic and axillary lymph nodes, liver foci, adrenal glands, thyroid gland, and the most intensely DOTATE-avid lesion was seen in the pancreas. Endoscopic Ultrasound with biopsy of pancreatic mass was done which revealed a Pancreatic Neuroendocrine Tumor Grade 2. Patient was given Lutetium-177 as treatment and after two doses, noted stable pancreatic mass with slight decrease in uptake in some hepatic lesions.
Discussion: The treatment of a pancreatic neuroendocrine tumor (pNET) is complex and depend on several factors, including the tumor's size, grade, stage, location, and the presence of metastasis. Because of the indolent course and nonspecific presentation of the disease, many patients are diagnosed with locally advanced disease at the onset- limiting treatment options. A high index of suspicion is necessary to diagnose and treat this disease early to prevent further complications.
PP-02-132
Utility of D-dimer, antithrombin-III, protein c in prediction of severity and prognosis of acute pancreatitis
NS Ravichandra and Vineet Chaudary and Sumaswi Angadi and Bhushan Chopade and Sukanya Bhrugumalla
Nizams Institute Of Medical Sceiences, Hyderabad, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: The study was conducted to assess the predictive value of D-dimer, antithrombin III, protein C, and other coagulation parameters in the severity and prognosis of acute pancreatitis.
Materials and Methods: This is a single-center, prospective observational study of patients admitted with acute Pancreatitis (AP) in a tertiary healthcare center in south India between January 2018 and January 2019. Platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen, antithrombin III (AT III), Protein C, and D-dimer were measured in plasma on day 0, and day 3 of admission. The severity of AP defined according to revised Atlanta classification. Outcomes like length of hospital stay, organ failure, and in-hospital mortality were analyzed.
Results: 61 patients included; 15 (25%) mild, 24(39%) moderately severe and 22 (36%) patients severe acute pancreatitis, out of which 9 (15%) patients died during the hospital stay. PT, D- dimer and fibrinogen levels were significantly higher whereas platelet count, protein C, and AT III were significantly lower among severe pancreatitis and non-survivors. Fibrinogen levels, TT, and APTT were not statistically different between survivors and survivors. Protein C, and AT III on day 3 at cut-off ≤72% and ≤82% respectively were better predictors of severe acute pancreatitis with an AUROC of 0.886, and 0.877 respectively. D- Dimer on day 0 at cut-off ≥ 10μg/ml was a better predictor of mortality with an AUROC of 0.881.
Conclusion: Coagulation parameters in the early phase can be utilized to predict the severity and prognosis of acute pancreatitis.
PP-02-133
Basic and clinical significance of epithelial membrane protein 1 (EMP1) in pancreatic ductal adenocarcinoma
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The expression of Epithelial Membrane Protein 1 (EMP1) has been reported in cases of various cancer types, with EMP1 identified as a potential prognostic biomarker in patients with pancreatic ductal adenocarcinoma (PDAC). However, its role in PDAC remains unknown. Therefore, we aimed to investigate the importance of EMP1 in PDAC.
Materials and Methods: Mouse cancer cell lines (KrasLSL-G12D; Trp53LSL-R172H; Pdx1-Cre: KPC mice), human cell lines (Panc-1 and MIAPaCa-2) and patient-derived organoids (PDOs) from patients with PDAC were used to investigate the functional role of EMP1. Bioinformatics analyses were performed using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus.
Result: We generated EMP1 knockout (KO) and overexpression cell lines in vitro and found high proliferative and metastatic potential drug resistance in the group with high EMP1 expression among all three models. In vivo, we found EMP1 was essential for the proliferation and metastasis of PDAC in mouse models using intrapancreatic and intrasplenic injection. Eleven PDOs were derived from patients with PDAC. EMP1 expression was significantly higher in the liver metastasis group than in the locally advanced groups. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed drug resistance in the group with high EMP1 expression. Mechanistically, RNA-sequencing of the EMP1 KO mouse model and analysis of the TCGA dataset revealed that K-RAS signaling, and epithelial-mesenchymal transition (EMT) were closely correlated with EMP1.
Conclusion: EMP-1 is associated with the proliferative and metastatic potential of PDAC through the K-RAS pathway and EMT and may be a future therapeutic target.
PP-02-134
Systematic Review of Research Progress on Borderline Resectable Pancreatic Cancer using Bibliometric and Visualized Analysis
Jae Keun Park1 and Ji Woong Hwang2
1Hallym University Kangnam Sacred Heart Hospital, Seoul, South Korea;2Chung-Ang University Gwangmyeong Hospital, Chung-Ang University College of Medicine, Gwangmyeong, Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Borderline resectable pancreatic cancer, an intermediate stage between a completely resectable state and an unresectable state, requires a multidisciplinary treatment approach. This study aimed to elucidate the main characteristics and recent research trends regarding borderline resectable pancreatic cancer to gain further insights into them.
Materials and Methods: Data from published papers about borderline resectable pancreatic cancer were collected from Web of Science (2014–2023) for the analysis. This study included 355 papers; data on major countries, publishing organizations, and keywords were collected and analyzed. Furthermore, R studio and VOSviewer were used for the qualitative and quantitative analyses of keywords.
Results: Publication of papers on borderline resectable pancreatic cancer was observed to be increasing annually by 12.8%, with the United States and Japan being the main publishing countries. In 2014, keywords related to surgery and chemotherapy were dominant; however, a shift toward more integrative approaches, such as neoadjuvant therapy, was observed over time.
Conclusion: This study demonstrates rapidly evolving trends and paradigm changes in the research and management of borderline resectable pancreatic cancer. Thus, the results of this study are expected to contribute to establishing future research strategies and improving patient treatment outcomes.
PP-02-135
Long term outcomes of splanchnic venous thrombosis in acute pancreatitis – A prospective cohort study
Sudipta Dhar Chowdhury, Samanvith Patlori, Betty Simon, Gauri Kumbhar, Reuben Thomas Kurien and Paul Deepak
Christian Medical College, Vellore, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Splanchnic venous thrombosis (SVT) is a serious complication of acute pancreatitis(AP). The natural history of SVT in AP is not well defined. Aim of this study aimed was assess the natural history of SVT in patients with AP.
Materials and Methods: Patients with AP admitted between October 2018 to September 2023 were included. Inclusion criteria -1) First episode of AP, 2)Presence of SVT. Patients with chronic pancreatitis, recurrent AP, cirrhosis, malignancy and follow-up l< 6 months were excluded. Baseline data of patients, aetiology, severity, imaging, anticoagulation details were collected. Doppler ultrasound was performed at ≥6 months to assess for re-canalization.
Results: 814 patients were admitted with AP during the study period. SVT was diagnosed in 92 patients. Of the 92 patients, 10 died, 9 were lost to follow up and 3 refused consent. 70 patients with SVT were included in the study. The mean age was 38.1(SD 12)years, 65(92.8%) were males. Predominant aetiology was alcohol(44 (62.8%) patients). 64(91.4%) had necrotizing pancreatitis. SVT was seen more frequently in retro-pancreatic splenic vein (47(67.1%)). 14(20%)patients received therapeutic anticoagulation. On follow up, complete recanalization was noted in 38(54.3%) and partial recanalization in 2(2.9%) patients. At univariate analysis on admission BISAP >2 was a risk factor for non-recanalization of SVT(p-0.007). Anticoagulation had no significant effect on recanalization rates in patients with SVT(64.3% with anticoagulation vs. 35.7% without anticoagulation, p=0.764).
Conclusion: Majority of patients who develop SVT in the setting of AP spontaneously recanalize on follow up. Severity of pancreatitis predicts non recanalization of SVT.
PP-02-136
Efficacy of thoracic epidural versus intravenous analgesia for pain control and morbidity in acute pancreatitis
BLK Max Superspeciality Hospital, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Primary objective was to assess pain control and secondary objectives were to assess efficacy of epidural and IV analgesia on organ failure,morbidity and mortality.
Materials & methods: Over a period of one year,all consecutive patients of AP with moderate to severe pain with numerical rating scale (NRS ≥4) were sequentially allocated into 2 groups-Epidural Patient controlled analgesia (PCA) (Ropivacaine + Fentanyl) and IV PCA (Fentanyl) + IV Paracetamol for 5 days.Morphine was used as rescue analgesia when NRS ≥4.
Results: We enrolled 36 patients (18 in each group).Baseline characteristics were comparable between two groups.The mean number of days with adequate pain control was 3.28 ± 0.75 days in epidural vs 3.11 ± 0.76 days in IV group (p = 0.496).Adequate pain control was achieved at day 3 in both groups.9(50%) Patients in IV group required rescue analgesia vs 7 (38.9%) in epidural group and the cumulative dose of rescue analgesia required was 60 mg in epidural group vs 76 mg in the IV group (p = 0.83).Most commonly observed organ failure was acute lung injury in 6 (16.7%),3 in each group (p = 1.0).The resolution of SIRS at day 3 in comparison to baseline was better in epidural group as compared to IV group (70% vs 27.7%) (p = 0.089).There was no mortality in either group.
Conclusion: Thoracic epidural PCA and IV PCA were equally efficacious in pain control in AP.Moreover,thoracic epidural analgesia was safe,feasible,required less rescue analgesia and showed an insignificant clinical trend towards early SIRS resolution.
PP-02-137
Purtscher-like retinopathy: A rare complication of acute on chronic pancreatitis
1Division of HPB/GI Surgeyr, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2The University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;3The National Eye Hospital, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Purtscher’s retinopathy is an occlusive micro-vasculopathy causing sudden onset visual loss. Similar retinal appearance is observed as a very rare complication of acute pancreatitis which is identified as Purtscher-like retinopathy(PLR). Here we present a patient with acute on chronic pancreatitis developed sudden unilateral blindness due to PLR.
Case Description: A 15-year-old female with idiopathic acute on chronic pancreatitis who noticed a sudden onset loss of vision in the left eye and was found to have significantly diminished visual acuity. A dilated fundoscopic examination demonstrated pathognomonic Purtscher flecken, multiple retinal haemorrhages, cotton wool spots and macular oedema. A clinical diagnosis of Purtscher-like retinopathy was made in the setting of acute on chronic pancreatitis. Optical coherence tomography was used to support the diagnosis and to monitor response to therapy. She was treated with intravitreal bevacizumab and triamcinolone immediately upon diagnosis.Although she had significant improvement on fundoscopy, she only had slight improvement vision from 1/60 to 2/60. Optical coherence tomography showed poor prognostic factors like disrupted inner segment/outer segment junction and macular atrophy.
Discussion: This is a rare vision threatening complication of acute pancreatitis often undiagnosed or underreported. Leuko-embolism and fat embolism casing retinal ischaemia are probable pathophysiological mechanisms. Given the variable prognosis with no evidence-based therapies available, she had a subjective improvement in visual acuity with administration of intravitreal steroids and observation with management of the acute episode of pancreatitis.Knowledge and awareness of this rare condition will enable its early detection and the search for newer therapies.
PP-02-138
A Recherche Entity: Pancreatic Tuberculosis In Non-Immunocompromised Patients: Case Reports And Review Of The Literature
Erika Johanna Tañada-Escanlar, Frederick Dy and Estrellita Ruiz
UST Hospital, Manila, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Pancreatic tuberculosis is rare and an even rarer occurrence in immunocompetent patients masquerading as pancreatic malignancy. We present two cases of pancreatic TB in a young and middle age woman presenting with abdominal pain and weight loss with no risk factor for immunosuppression and we review the literature to describe the diverse clinical manifestations and diagnosis of this condition.
Case presentation: A 22-year-old female presented with 1 month history of abdominal pain and weight loss and denied fever, night sweats, constitutional symptoms or tuberculosis contact. On examination, there was no palpable mass per abdomen with normal CA-19-9 (9 U/ml). Ultrasonography revealed 2.2 x 5.5 cm complex mass on pancreatic head, contrast-enhanced computed tomography (CT) of the abdomen revealed 4x5 cm pancreatic head mass with paraaortic lymphadenopathy. Another 43-year-old female with also no history of tuberculosis contact and immunosuppression presented with two months abdominal pain and weight loss. Her abdomen CT scan revealed a 8x3x5 cm hypoenhancing hypodense pancreatic head lesion. Two patients underwent EUS with FNA biopsy which showed chronic caseating granulomatous inflammation with microabscesses and their pancreatic tissue geneXpert were positive for mycobacterium tuberculosis. The patients were subsequently started on antitubercular medication and recovered well.
Conclusion: Notwithstanding the fact that some case reports have been published, a high index of suspicion is still needed to diagnose pancreatic tuberculosis especially in immunocompetent patients. EUS-guided fine-needle aspiration is the diagnostic modality of choice and pancreatic geneXpert can be a useful tool for the diagnosis of pancreatic tuberculosis.
PP-02-139
Predictors of Pain Relapse after Ductal Interventions in Chronic Pancreatitis: A Machine Learning-Based Approach
Misbah Unnisa1, Abdul Rasheed1, Madhurya Reddy Chinthakuntla2, Rajesh Goud1, D. Nageshwar Reddy1 and Rupjyoti Talukdar1
1Asian Institute Of Gastroenterology (AIG) Hospitals, Hyderabad, India;2KU School of Medicine, Wichita, USA
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: While ductal interventions can improve pain in chronic pancreatitis (CP), their impact on disease progression remains unclear. We aimed to develop machine learning-based models to identify clinical parameters that predict pain recurrence after ductal interventions.
Materials and methods: In this cross-sectional study, consecutive patients with documented CP were screened and enrolled between August 2022 and November 2023. Clinical, laboratory, imaging, and treatment data were recorded. 18-Preintervention independent-clinical predictors were included with dependent variable as pain relapse after initial improvement. The dataset was split into 80% training and 20% testing. Machine Learning(Python.3) algorithms namely DecisionTree, LogisticRegression, RandomForest, Support-Vector-Machine, CatBoost, and XGBoost were employed. Models performed evaluation metrics mainly AUROC, accuracy and Root Mean Squared Error(RMSE). SMOTE and simple imputer addressed data imbalance and missing values, respectively. Bootstrapping estimated the confidence interval.
Results: 353 patients were recruited, and the final prediction model was based on 247 patients (Mean age 35.24±12.51 years;65.04% male) details before interventions. Most common etiology was idiopathic (66.39%). On the testing dataset, Logistic regression emerged as the top performer with an accuracy of 86% with AUROC of 0.904(95% CI: 0.798-0.98) and its RMSE was 0.374. CatBoost accuracy was 0.76 and AUROC of 0.754(95%:0.603-0.892) with RMSE was 0.489. Highlighted predictors were depression, long-standing diabetes, spontaneous pain, piercing, dragging and pressure nature of pain as consistently influential across models, underscoring their importance in predicting the outcome.
Conclusion: Based on Logistic Regression model, we identified key predictors of pain relapse after ductal Interventions, that to be tested in large multicentre cohorts.
PP-02-140
Oxidative balance score and gallstones:Lesson from NHANES
Bo Wang1, Yongqiang Xiong1, Na Huang2, Jun Li2 and Shu Zhang1,3
1Department of Geriatric General Surgery, The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;2National & Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;3Experimental Teaching Center for Clinical Skills, The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Gallstones pose a significant burden on public health. The Oxidative Balance Score (OBS) integrates dietary and lifestyle factors to evaluate antioxidant exposure and oxidative stress. However, the relationship between OBS and gallstones is not well understood.
Methods: To investigate the association between OBS and gallstones, we analyzed the data from National Health and Nutrition Examination Survey (NHANES) and employed a weighted multivariable logistic regression model, supplemented by subgroup analyses. Mendelian randomization (MR) was conducted to explore the causal relationships between OBS components and gallstones, using inverse variance weighting (IVW) as the primary analytical method. Sensitivity analyses were also performed.
Results: The observational study found a significant inverse correlation between OBS and gallstones. Compared to the first quartile (Q1), the risk of gallstones was reduced by approximately 25% in the third quartile (Q3) (OR: 0.742, 95% CI 0.589-0.933, P = 0.011) and by about 31% in the fourth quartile (Q4) (OR: 0.693, 95% CI 0.549-0.873, P = 0.002). Subgroup analyses revealed that this association was stronger in individuals under 60 years, females, married individuals, and those with a college education or higher. MR analysis indicated a causal relationship between certain OBS components—namely vitamin B12, physical activity, BMI, and smoking—and the risk of gallstones, supported by sensitivity analyses.
Conclusion: A high OBS is associated with a reduced risk of gallstones, particularly among individuals under 60 years, females, married individuals, and those with higher education levels. Key mediating factors include vitamin B12, physical activity, BMI, and smoking.
PP-02-141
The association between sarcopenia and gallstones: A cross-sectional study from NHANES
Bo Wang1, Qianxi Huang2, Yongqiang Xiong1 and Shu Zhang1
1The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, China;2Yangzhuang Community Health Center of Chang'an District, Xi’an, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Sarcopenia is an age-related condition characterized by a decline in muscle mass, strength, and function. However, its relationship with gallstones remains unclear.
Methods: This cross-sectional study included 2,167 participants from the 2017-2018 National Health and Nutrition Examination Survey (NHANES). Sarcopenia was assessed using the sarcopenia index, and the association between sarcopenia and gallstones was evaluated using multivariable logistic regression models and restricted cubic spline (RCS) regression for dose-response curve analysis. Additionally, subgroup analyses and propensity score matching (PSM) were conducted to account for potential confounding factors.
Results: We found a significant negative association between the sarcopenia index and the risk of gallstones (OR: 0.253, 95% CI: 0.132-0.471, P < 0.001). In Model 4, which integrated all covariates, sarcopenia was associated with approximately a 100% increased risk of gallstones compared to non-sarcopenia patients (OR: 1.995, 95% CI: 1.340-2.948, P < 0.001). The results from propensity score matching also confirmed sarcopenia as an independent risk factor for gallstones (OR: 1.982, 95% CI: 1.217-3.285, P = 0.007). Notably, this association was more pronounced in subgroups including females, non-Hispanic whites, married individuals, those with college education or higher, and in populations without diabetes, liver disease, or cancer.
Conclusion: Our study suggests a significant association between sarcopenia and increased risk of gallstones among U.S. adults. This finding warrants validation through further large-scale prospective studies.
PP-02-142
rIL-22 alleviates severe acute pancreatitis and secondary multiple organ injury induced by cerulein in mice
Hongli Yang and Weijia Sun and Feifei Zhou and Hongwei Xu
Provincial Hospital affiliated to Shandong First Medical University, Jinan, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Severe acute pancreatitis (SAP) is characterized by systemic inflammation that can lead to multiple organ failure and even death. However, effective treatment strategies are lacking. IL-22 has been shown to have anti-inflammatory effects. This study aims to investigate the therapeutic effects of rIL-22 on SAP and secondary multiple organ injury mice.
Materials and methods: A mouse SAP model was constructed by intraperitoneal injection of cerulein and lipopolysaccharide, and then intraperitoneal injection of rIL-22. Peripheral blood, pancreas, liver, kidney, and lung tissues were collected. ELISA was performed to measure the levels of serum related indicators (AMY, LPS, ALT, AST, CRE, BUN, TNF-α, IL-6, and IL-1β). Histopathological changes were observed by HE staining. TUNEL assay was conducted to assess apoptotic cells. Immunofluorescence and Western blot were used to examine levels of Bax, caspase-3, and BCL2.
Results: Compared to the NS group, HE staining of the SAP group revealed pancreatic acinar necrosis , disordered hepatocyte arrangement, alveolar wall swelling, and renal tubular epithelial cell necrosis. The levels of serum related indicators were elevated. Bax and caspase-3 levels were increased, while BCL2 was decreased. For rIL-22 group, HE staining showed reduced damage in the pancreas, liver, lung, and kidney tissues. The levels of serum related indicators were decreased. The expression of Bax and caspase-3 were decreased, and increased BCL2.
Conclusion: rIL-22 can alleviate systemic inflammation and subsequent multiple organ injuries in SAP mice, providing a novel option for future SAP treatment.
PP-02-143
Single cell RNA sequencing revealed the role of Reg3 in acinar injury in acute pancreatitis
Pan Zheng, Nianshuang Li and Yin Zhu
The first affiliated hospital of Nanchang University, Nanchang, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Acute pancreatitis is a common acute abdominal condition that can rapidly progress to severe states with high mortality rates. To investigate the pathogenesis of acute pancreatitis, we utilized single-cell transcriptomic sequencing to delve into the cellular landscape comprehensively and transcriptomic changes in cerulein-induced AP.
Material and methods: Single-cell transcriptomic sequencing was employed to analyze cell-type compositions and their transcriptomic landscapes in mice with AP and healthy controls. Immunohistochemistry, immunofluorescence, qPCR, and western blotting were applied to validate significant differentially expressed genes among homotypic cells. Cerulein and pancreatic duct ligation induced AP in REG3A-/-, REG3B-/-, and REG3G-/- mice.
Results: Following the onset of AP, the proportion and transcriptional profiles of pancreatic cells underwent a remarkable shift. As expected, inflammatory cells such as neutrophils and macrophages significantly increased in the AP group. Acinar cells during AP presented distinct transcriptomic expression profiles compared to normal acinar cells. Apart from the elevated expression of pancreatic enzyme genes, REG3A, REG3B, and REG3G genes also exhibited a significant increasing trend in acinar cells of AP. After validation of upregulated expressions of REG3A, REG3B, and REG3G in different AP mice models, we induced AP in REG3A, REG3B, and REG3G knockout mice and found a noticeable increase in the severity of murine AP, mainly manifested by extensive acinar necrosis.
Conclusion: The pancreatic cells of mice with AP display distinct transcriptomic profiles and cell population proportions compared to healthy mice. The upregulation of REG3A, REG3B, and REG3G genes in acinar cells during AP plays a significant protective role.
PP-02-144
Comparison of de ritis, gamma-gt/albumin, alkaline-phospahatase/albumin ratios in obstructive jaundice patients with and without malignancy
Muhammad Naufal Zuhdi1, Numan AS Daud2,3, Susanto Hendra Kusuma2,3, Fardah Akil2,3, Muhammad Luthfi Parewangi2,3, Rini Rahmawarni Bachtiar2,3 and Amelia Rifai2,3
1Internal Medicine Specialist Program, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;2Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;3Centre of Gastroenterology-Hepatology HAM Akil, Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The De Ritis ratio [Serum Glutamic Oxaloacetic Transaminase (SGOT)/ Serum Glutamic Pyruvic Transaminase (SGPT)] has shown that its elevated ratio is a risk factor in several solid tumors. Gamma GT/Albumin ratio (GAR) and Alkaline Phosphatase/Albumin ratio (APAR) also have been widely used as biomarker parameters to assess the prognosis of patients with solid tumors. This study aims to investigate the comparison of De Ritis ratio, GAR, and APAR in patients with obstructive jaundice with and without malignancy.
Materials and methods: This study was conducted with a retrospective case control approach. The study population consisted of patients with obstructive jaundice in Wahidin Sudirohusodo Hospital which had undergone Endoscopic Retrograde Cholangiopancreatography (ERCP) procedure from January to June 2024. Malignancy diagnosed based on tumor markers and histopathology results. De Ritis Ratio, GAR and APAR were examined prior to the procedure.
Results: We involved 62 subjects who had undergone ERCP for diagnosing obstructive jaundice due to either malignant with 25 (40%) subjects (56.2 ± 10.5 SD years old) or non-malignant with 37 (60%) subjects (45.8 ± 14.3 SD years old). Elevated enzymes concentration was identified in the entire of estimated parameters and decreased albumin serum concentration in both groups. These ratios consistently showed higher ratio in the malignant than in the non-malignant group. Statistical analysis estimated GAR value was significantly higher in obstructive jaundice caused by malignancy (Averaged GAR malignant = 123.3, 95% CI 85.8-160.8; p = 0.03, independent t-test).
Conclusion: GAR is higher in obstructive jaundice because of malignancy.
PP-02-145
Predictive value of TyG index and VAT-related index for severity of hypertriglyceridemic pancreatitis
Rongjin Gu and Yuerong Zhang
Department of Gastroenterology, University-Town Hospital of Chongqing Medical University, Chongqing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To investigate the relationship of triglyceride glucose (TyG) index and visceral adipose tissue-related(VAT) indices with hypertriglyceridemic pancreatitis (HTGP) and its predictive value for disease severity.
Materials and methods: Retrospective study of a total of 281 patients with acute pancreatitis admitted to the University-Town Hospital from 2018 to 2023,and divided into two groups(HTGP and the non-HTGP group), each group was divided into the mild acute pancreatitis (MAP), moderately severe and severe acute pancreatitis (MS-SAP).Comparison of VAT-related indices(including Chinese visceral adiposity index (CVAI),visceral adiposity index (VAI),lipid accumulation product (LAP))and TyG index between two groups.The predictive value of TyG index and VAT-related indices on the severity of HTGP was evaluated using the ROC curve.
Results: The VAT-related indices and TyG index in the HTGP group were higher than those in the non-HTGP group(p <0.001). The VAT-related indices and TyG index in the MS-SAP group were significantly higher than that in the MAP group(p <0.001).The ROC curve showed that the TyG index had the highest predictive efficacy for predicting HTGP disease severity, with a maximum cutoff value of 11.30 (AUC = 0.734,95% CI: 0.655 ~ 0.812, P <0.001).
Conclusion: The VAT-related indices and TyG index were significantly higher in the HTGP group than in the non-HTGP group, with significant differences between the two groups, and the VAT-related indices and TyG index were positively correlated with the severity of HTGP. TyG index has the greatest value in predicting the severity of HTGP, which is better than other indexes.
PP-02-146
Hematemesis melena in duodenal tuberculosis: a case report
Hendra Asputra and Hasan Maulahela
Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Primary duodenal tuberculosis is a very rare case with a prevalence of only 0.04% of all tuberculosis cases and 4.7% of all cases of intestinal tuberculosis. The lack of specificity of the symptoms makes duodenal tuberculosis difficult to diagnose which can lead to late treatment or complications.
Case Presentation: A 61-year-old male patient came with complaints of vomiting blood and black stool since 3 weeks ago. The patient complained of heartburn, nausea and bloating since 6 months ago. During the last 1 month, the patient often vomit a few hours after eating. The patient has lost 10 kg in the last 6 months. There was no history of coughing up blood. The patient has a history of diabetes mellitus but was not compliant to treatment
The results of the physical examination showed anaemic conjunctiva and epigastric tenderness. Lab result showed hemoglobin 3.0. CT scan showed thickening of the wall of ascending duodenum with a dense mass of contrast. Esophagastroduodenoscopy resulted in lobulated, semicircular, fragile and bleeding mass in the duodenum which was suspected to be malignant. The results of the colonoscopy was grade 2 internal hemorrhoids. CEA tumor markers are within normal limits. Histopathological result was datia langhans cells and concluded chronic inflammation of tuberous granulomatose. The final diagnosis was tuberculosis of the ascending duodenum pars with bleeding.
Discussion: Duodenal tuberculosis is rare and difficult to diagnose clinically. Endoscopy has an important role in the establishment of a proper diagnosis.
PP-02-147
Real-World Bleeding Risk of Anticoagulant and NSAID Combotherapy Versus Anticoagulant Monotherapy
Jae Myung Cha and Moonhyung Lee
Kyung Hee University Hospital At Gangdong, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The incidence of acute gastrointestinal bleeding (GIB) increases with the utilization of anticoagulants and nonsteroidal anti-inflammatory drugs (NSAIDs). This study aimed to compare the risk of GIB between anticoagulant and NSAID combo therapy and anticoagulant monotherapy in real-world practice.
Materials and methods: We investigated the relative risk of GIB in individuals newly prescribed anticoagulant and NSAID or anticoagulant monotherapy at three hospitals using ‘Common Data Model’. Cox proportional hazard models and Kaplan-Meier estimation were employed for risk comparison after propensity score matching.
Results: A comprehensive analysis of 2,951 matched pairs showed that anticoagulant and NSAID combo-users exhibited a significantly higher risk of GIB compared to anticoagulant mono-users (hazard ratio [HR]=1.66; 95% confidence interval [CI]: 1.30–2.12; p<0.001). The risk of GIB associated with the combo-therapy of anticoagulant and NSAID was also significantly higher than that of anticoagulant monotherapy in elderly patients >65 years (HR=1.53; 95% CI: 1.15–2.03; p=0.003) and >75 years (HR=1.89; 95% CI: 1.23–2.90; p=0.003). We also found that the risk of GIB was significantly higher in the anticoagulant and NSAID combo-users than that of anticoagulant mono-users in both male (p=0.016) and female cohorts (p=0.010).
Conclusion: The risk of GIB is significantly higher in anticoagulant and NSAID combo-users than anticoagulant mono-users. In addition, the risk of GIB associated with the combotherapy of anticoagulant and NSAID was much higher in elderly individuals >75 years. Therefore, physicians should pay more attention to the risk of GIB when they use anticoagulants and NSAIDs.
PP-02-148
Abnormal gastroesophageal flap valve: a predictor of recurrent variceal hemorrhage
Jay Chudasama
Topiwala National Medical College, Mumbai, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Aim: To investigate the relationship between GEFV and esophageal variceal bleeding episodes.
Methods: In this cross-sectional, retrospective study, 300 consecutive patients with esophageal varices and a documented GEFV during esophagogastroduodenoscopy were included. Patients were divided into two groups according to: the Hill’s grade of flap valve (grade 1,2- normal and grade 3,4- abnormal), size of varices - large (>5 mm) and small (<5 mm) and the number of bleeding episodes into: Group A with ≤ 1 and Group B with ≥ 2 bleeding episodes. We compared GEFV and various other factors to the number of variceal bleeding episodes.
Results: 224 patients (74.60%) had a normal and 76 (25.40%) had an abnormal GEFV. Clinical variables were statistically significant in the abnormal GEFV group (P<0.0.5). Propensity score matching was done to reduce the significant differences in the clinical background at baseline between the 2 groups. 152 patients (76 in each group) were analysed after propensity score matching. A significant difference between the two groups disappeared except for number of bleeding episodes Binary logistic Cox regression analysis was applied using the clinical variables to assess their role in predicting recurrent variceal bleeding. On univariate analysis, abnormal GEFV and large varices were significantly associated with recurrent esophageal variceal bleed (P =0.001). On Multivariate analysis, abnormal GEFV (OR 7.25, P = 0.001) and large varices (OR 5.70, P = 0.001) were independent predictors for recurrent esophageal variceal bleeding
Conclusion: Abnormal GEFV and large varices are independent risk factors for recurrent esophageal variceal haemorrhage.
PP-02-149
Validating the CHAMPS Score: A Novel and Reliable Prognostic Score of Non-Variceal Upper Gastrointestinal Bleeding
Thong Duy Vo and Huong Tu Lam and Thang Dinh Nguyen and Hoang Huu Bui
University Medical Center Ho Chi Minh City, Ho Chi Minh, VietNam
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The CHAMPS score is a newly developed prognostic tool for assessing mortality risk in acute non-variceal upper gastrointestinal bleeding (NVUGIB). This study aims to externally validate the CHAMPS score and compare its predictive accuracy with established scoring systems.
Materials and methods: A prospective cohort study was conducted involving 140 adult patients with NVUGIB admitted to the Department of Gastroenterology at Cho Ray Hospital, Ho Chi Minh City, from November 2022 to June 2023. The performance of the CHAMPS score in predicting in-hospital mortality and other clinical outcomes was assessed using area under the receiver operating characteristic (AUROC) curves. Comparative analyses were performed against the GBS, AIMS65, cRS, fRS and ABC score.
Results: The CHAMPS score demonstrated superior performance in predicting in-hospital mortality with an AUROC of 0.89, significantly outperforming the GBS and AIMS65 scores (AUROC = 0.72 and 0.71, respectively; p < 0.05). The optimal CHAMPS score cut-off for predicting mortality was 3 points, with a sensitivity of 100% and specificity of 71.2%. Subgroup analysis confirmed its robust predictive capabilities for both bleeding-related and non-bleeding-related mortality (AUROC = 0.88 and 0.87, respectively). However, the CHAMPS score had limited predictive value for rebleeding and intervention (AUROC = 0.43 and 0.55, respectively).
PP-02-150
The Correlation Of Pre-Endoscopic Glasgow Blatchford Score With Post-Endoscopic Forrest Classification Of Peptic Ulcer Disease
Angela Gabasan, Rolan-Ohmar Yumul and Jan Michael Kong
Victoriano Luna Medical Center, Quezon City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To validate the Glasgow Blatchford Scoring System by determining its correlation with the post-endoscopic Forrest Class in patients with upper gastrointestinal bleeding.
Materials and methods: It employed a retrospective approach to validate pre-endoscopic Glasgow Blatchford Scoring system clinical outcomes of patients’ upper gastrointestinal bleeding using the Forrest classification. The medical records of 50 patients admitted at Victoriano Luna Medical Center from January 2018 until December 2019 were rigorously reviewed from records and recorded for the comparison and analysis of data. The chi-square test and Fisher’s exact test (two-sided) will be used to compare categorical variables.
Results: A negative correlation was observed between Glasgow Blatchford Scoring System and Forrest Classification based on Chi Square and Fischer Exact Test (p .653 > .05). The Glasgow Blatchford score had a sensitivity of 80% with a specificity of 15%. The positive predictive value was at 19% and the negative predictive value of Glasgow Blatchford score was at 75%.
Conclusion: This study revealed that the Glasgow Blatchford scores could be used as a tool for identifying patients who may be at high risk and may need more intensive intervention, and those patients who could be safely discharged. This conclusion was based on the results of this study that showed that this risk stratification tool had a high sensitivity for detecting high-risk ulcers, and a high negative predictive value indicating a low likelihood that patients assessed to have low-risk disease by GBS would have high-risk ulcers.
PP-02-151
Utilization of Hemostatic Powder Spray versus Standard Endoscopy for Malignancy-Related GI Bleeding: A Meta-Analysis
Laurence Laurel, Ziggy Amores and Roel Galang
St. Luke's Medical Center-Global City, Taguig, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Managing tumoral bleeding is challenging for endoscopists. Traditional tools like clips and heat-probes often fail to control bleeding caused by mucosal invasion from tumors. Hemostatic powder sprays offer a novel solution by forming a mechanical barrier on the tumor surface to seal the bleeding site. However, data on their effectiveness in treating hemorrhagic gastrointestinal neoplasms is scarce. This study evaluates the efficacy of Hemostatic powder spray in patients with gastrointestinal bleeding related to malignancies.
Aims And Methods: This study aimed to evaluate the effectiveness of Hemostatic powder spray compared to standard treatment in controlling gastrointestinal bleeding from malignant lesions. The primary outcome was rebleeding rates within 30 days, while the secondary outcome was overall mortality.
A comprehensive search of electronic databases was conducted up to April 2024, including only randomized controlled trials (RCTs) where Hemostatic powder spray was compared to conventional endoscopic techniques for managing gastrointestinal tumoral bleeding.
Results: Three studies involving 162 patients were included. Among them, 83 patients received Hemostatic spray powder, while 79 received conventional hemostatic techniques. The analysis revealed that Hemostatic powder spray was associated with a lower incidence of re-bleeding beyond 30 days (odds ratio [OR] 0.36, 95% confidence interval [CI] 0.15-0.84), showing a significant advantage over standard endoscopy (p-value 0.01). No significant difference in overall mortality was observed between the two groups (OR 0.40, 95% CI 0.19-0.83, p-value 0.09).
Conclusion: Hemosprays are effective in achieving hemostasis for gastrointestinal bleeding associated with malignancies. They can be considered a viable bridge to surgery and/or embolization.
PP-02-152
Endoscopic Evaluation of Upper Gastrointestinal Bleeding: Five-Year Study at Cipto Mangunkusumo National Referral Hospital
Virly Muzellina1, Liovicinie Andarini2 and Ari Fahrial Syam1
1Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia;2Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate the causes of hematemesis and melena over the past five years, with a focus on improving patient outcomes and guiding clinical decisions through endoscopic examination.
Methods: The study analyzed records of patients retrospectively who underwent upper gastrointestinal endoscopy at the Gastrointestinal Endoscopy Center in Cipto Mangunkusumo Hospital, Jakarta, Indonesia between 2019 and 2023.
Results: Between 2019 and 2023, 5,721 patients underwent upper gastrointestinal endoscopy, with 1,090 (19.1%) due to upper gastrointestinal bleeding. Among these, 593 were male (54.4%) and 497 were female (45.6%), with a mean age of 54.4 ± 15.12 years. The largest age group affected was 40-59 years, comprising 451 cases (41.4%). Of the 1,090 patients, 262 (24%) presented with hematemesis, 654 (60%) with melena, and 174 (16%) with both symptoms. Non-variceal bleeding was identified in 913 cases (83.8%), while variceal causes accounted for 177 cases (16.2%). Erosive gastritis was the most frequent cause of upper GI bleeding (408 cases, 37.4%), followed by peptic ulcers (270 cases, 24.8%). Among peptic ulcer cases, 136 (50.4%) were located in the stomach. Additionally, cancer was a cause in 104 cases (9.5%), with duodenal cancer being the most common (48 cases, 46.2%).
Conclusion: This study identified erosive gastritis as the leading cause of upper gastrointestinal bleeding, followed by peptic ulcers and varices.
PP-02-153
Haematemesis and melena from esofageal varices in Banti’s Syndrome : Rare Case
Anton Purnomo and Febry Rahmayani and Suyata Suyata
RSUP dr. Mohammad Hoesin Palembang, Palembang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Abstract
Introduction: Haematemesis and melena are bleeding from the GI tract proximal to the ligament of Treitz. Non-cirrhotic portal hypertension is portal hypertension without cirrhosis, occurring in 10% of portal hypertension patients. Ultrasound examination and liver biopsy showed normal results. This disease is not easy to diagnose because of its low prevalence and varied clinical manifestations, generally in the form of esophageal varices, splenomegaly, and anemia. Banti syndrome is characterized by splenomegaly, pancytopenia due to hypersplenism, and portal hypertension, in the absence of liver abnormalities.
Case Presentation: A 24 year old man was diagnosed, coming with complaints of vomiting blood and black stools since 8 hours before entering the hospital. The patient had no history of hepatitis, diabetes mellitus, and hypertension. The patient also never smoked, drank alcohol or used illegal drugs. Physical examination revealed pale palpebral conjunctiva and hepatosplenomegaly. From laboratory data, the Hb level was 9.3 g/dL, leukocytes 3,800/mm3, and platelets 70x103/mm3. A CT scan of the abdomen showed hepatomegaly, dilated portal veins and diffuse permagna splenomegaly with dilated splenic veins. spleen stiffness was found to be 60 kPa. After an endoscopic procedure, a picture of grade III esophageal varices and portal hypertensive gastropathy was obtained. Bleeding was stopped by ligation of the varices and administration of intravenous terlipressin.
Conclusion: Banti’s syndrome is characterized by splenomegaly, pancytopenia due to hypersplenism, and portal hypertension in the absence of liver disease. Banti syndrome is a diagnosis of exclusion when all causes of portal hypertension, splenomegaly, and pancytopenia are excluded.
PP-02-154
Case series for the use of lanreotide in haemorrhagic hereditary telangiectasia (HHT) gastrointestinal bleeding
Farah Jaffar and Chehkuan Tai
Barnet General Hospital, Royal Free NHS Foundation Trust, London, United Kingdom
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Haemorrhagic hereditary telangiectasia (HHT) can lead to severe anaemia due to gastrointestinal (GI) angiodysplasia and associated chronic bleeding. This can result in repeated hospital admissions for transfusions and endoscopic evaluation which can have a significant impact on morbidity and mortality.
Case description: A 43 year old female with HHT complicated by GI angioectasia, pulmonary and hepatic arteriovenous malformations, had been significantly impacted by GI bleeding for over 10 years with the first angioectasia identified in 2013. Since then she has had annual endoscopies and was transfusion dependent due to severe anaemia. Most recently in June 2022 she had 173 angioectasias ablated with argon plasma coagulation (APC). She was subsequently started on a somatostatin analogue, lanreotide, in 2023 and has been free of hospitalisations, transfusions and endoscopic management since. A 62 year old female also with a background of HHT and duodenal and stomach angioectasia had recurrent hospital admissions with UGI bleeding resulting in endoscopic therapy with APC as well as clips. Her case is complicated by atrial fibrillation for which she cannot be anti-coagulated due to the frequent GI bleeding; this unfortunately resulted in a cerebrovascular accident leaving her with significant disabilities. Once initiated on lanreotide in February 2024, she has had no hospitalisations with GI bleeding.
Discussion: These cases highlight the significant burden that HHT GI bleeding has on patients due to the multiple admissions, transfusions and endoscopies impacting their quality of life. The introduction of somatostatin analogues is seen to be an effective and safe pharmacological option.
PP-02-155
Efficacy of transcatheter arterial embolization for nonvariceal upper gastrointestinal hemorrhage
Pham Phuong Thu Tran and Ngoc Luu Phuong Tran
Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Viet Nam
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Upper gastrointestinal bleeding (UGIB) is common emergency illness. Approximately 5% of patients experiencing recurrent bleeding after endoscopic therapies, facing the high death threats due to the limitations of current endoscopic equipment and techniques and patients with unstable comorbidity. The previous evidence illustrated that transcatheter arterial embolization (TAE) stood out as a safer and more effective alternative to surgery for these cases. This study aim to assess the efficacy of TAE within 7 days post-procedure in Vietnam.
Materials and Methods: Cases study of patients ≥ 18-year-old who had nonvariceal UGIB with TAE treatment from January 1st, 2019, to September 30th, 2022, at Gia Dinh and Trung Vuong hospital.
Results: We collected 64 nonvariceal UGIB cases treated by TAE. The most common bleeding site was the duodenal bulb (54.59% of cases). Ulcers larger than 1 cm were found in 59.38% of cases. Clearly bleeding evidence experienced in 78.12% of cases on endoscopy or abdominal CT. The technical and clinical success rate of TAE procedure were 96.87% and 73.44%, respectively. Serious complications accounted for 3.13%, including severe hypotension and liver ischemic. The in-hospital mortality rate was significant higher in patients with recurrent UIGB after TAE (OR 4.32, p = 0.02).
Conclusions: Refractory nonvariceal UIGB occurs in patients with complex etiology of bleeding and serious medical history. Give its efficacy and safety, TAE should be considered to manage bleeding after unsuccessful endoscopic therapy. For future work, the proposed method should be compared with surgery to determine the optimal approach for these patients.
PP-02-157
Safety and efficacy of linaclotide in treating constipation in pediatric patients: a systematic meta-analysis
Ahmed Farag1, Mohamed Karam Allah Elkholy2, Wisam Al-Khaboori3, Amro Mamdouh Abdelrehim1 and Safia Elshennawy4
1College Of Medicine, Misr University For Science And Technology, 6th October city, Egypt;2College of Pharmacy, Zagazig University, Zagazig, Egypt;3College of Medicine, Mutah University, Al-Karak, Jordan;4Shebin Elkom Teaching Hospital, Egypt
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Constipation is common in children and can be challenging to manage, especially in cases like functional constipation. Linaclotide, originally approved for adults, is now the only FDA-approved treatment for children with functional constipation. We aim to assess its safety and efficacy in this systematic meta-analysis.
Materials and Methods: Our literature search covered MEDLINE via PubMed, Scopus, WOS, and Cochrane. We only included RCTs. Primary outcomes spontaneous bowel movements (SBM) and treatment-emergent adverse events (TEAEs).
Results: Four studies with 639 patients were included. The overall effect estimates regarding SBM showed statistically significant results favoring linaclotide group [MD 1.09, 95 % CI (0.55–1.63), P = 0.0001] with a low heterogeneity (P = 0.29; I² = 19 %). Regarding Straining severity and stool consistency, the overall effect estimates favored linaclotide group [MD -0.39, 95 % CI (-0.57–-0.20), P = 0.0001], [MD 0.79, 95 % CI (0.32–1.26), P = 0.0009] respectively.
Regarding linaclotide safety in pediatric population, no statistically significant difference between groups were observed regarding any TEAEs [RR 1.13, 95 % CI (0.71–1.81), P = 0.61], any treatment-related TEAEs [RR 2.16, 95 % CI (0.89–5.22), P = 0.09], any serious adverse events [RR 0.69, 95 % CI (0.13–3.63), P = 0.66], or any adverse events leading to discontinuation [RR 0.75, 95 % CI (0.19–3.02), P = 0.69].
Conclusion: This is the first meta-analysis on linaclotide for pediatric constipation. Our results suggest that linaclotide demonstrates promising efficacy and is generally well-tolerated. Current evidence is insufficient; further high-quality, large-scale randomized controlled trials are required.
PP-02-158
Revisiting the Starting Age of Colorectal Cancer Screening for Average-Risk Asian Population: a cost-effectiveness analysis
Junjie Huang1, Victor Chan1, Mingtao Chen1, Jamie Jie Mei Liew1, Xiangjing Liu2, Chaoying Zhong3, Jianli Lin4, Junjie Hang5, Claire Chenwen Zhong1, Jinqiu Yuan6, Wanghong Xu7, Mellissa Withers8, Andrew Chan9 and Martin Wong1
1The Chinese University Of Hong Kong, Sha Tin, Hong Kong;2Erasmus MC University Medical Center, The Netherlands;3Guangdong Ocean University, China;4Peking University, China;5Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China;6Sun Yat-Sen University, China;7Fudan University, China;8University of Southern California, United States;9Massachusetts General Hospital, United States
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and aims: This study aims to assess the cost-effectiveness of different starting ages for colorectal cancer (CRC) screening among an Asian population.
Methods: We conducted a simulation study involving 100,000 individuals in Hong Kong who were screened using either fecal immunochemical test (FIT) or colonoscopy as primary screening methods at ages 40, 45, and 50 until age 75. Individuals with a positive FIT result subsequently underwent colonoscopy. The performance of different strategies was evaluated based on life-years gained, and cost-effectiveness was measured using the incremental cost-effectiveness ratio (ICER).
Results: The ICERs for initiating FIT screening at ages 40, 45, and 50 were USD 134,887, USD 108,178, and USD 87,362, respectively. For colonoscopy, the ICERs for initiating screening at ages 40, 45, and 50 were USD 592,767, USD 501,748, and USD 432,029, respectively. Overall, the FIT strategy was found to be less costly. The highest number of life-years gained was observed when colonoscopy screening was initiated at age 40 (2,070), followed by age 45 (1,923) and age 50 (1,714). In terms of FIT, the most life-years gained were observed at age 40 (1,652), followed by age 45 (1,515) and age 50 (1,323).
Conclusion: Initiating CRC screening at age 45 using FIT in Hong Kong was determined to be a well-balanced and cost-effective strategy. This approach demonstrated a cost advantage over starting screening at age 40 and resulted in more lives saved compared to screening at age 50.
PP-02-159
Incidence of venous thromboembolism in Asian patients with inflammatory bowel disease: systematic review and meta-analysis
1Department of Internal Medicine, Konyang University College of Medicine, Daejeon, South Korea;2Department of Biomedical Informatics, College of Medicine, Konyang University, Daejeon, South Korea;3Department of Internal Medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and aims: Although the incidence and prevalence of inflammatory bowel disease (IBD) have been gradually increasing throughout Asia, the incidence of venous thromboembolism (VTE) in Asia is relatively lower than that in Western countries. This study aimed to evaluate the incidence of VTE in Asian IBD patients using a systematic review and meta-analysis.
Methods: Studies were identified through a literature search of the PubMed, Embase, and Cochrane databases (from inception inclusive April 2024) for English studies. The criteria for selecting the participants were as follows: (1) studies including patients with Crohn's disease (CD) and Ulcerative colitis (UC) in the Asian population; (2) comparisons were specified as with a control group of non-IBD patients for comparative incidence; and (3) outcomes were measured by relative risks (RR) and hazard risk (HR) for VTE incidence in nationwide cohort studies.
Results: Five studies met the inclusion criteria. The pooled RR for overall VTE incidence in Asian patients with IBD compared to that in non-IBD patients was 2.065 (95% CI: 1.905–2.238). The heterogeneity test resulted in a p-value of 0.418 for the Cochrane Q statistics, and Higgins’ I2 was 0%. There was no statistical moderating effect of the variables (number of patients, mean age, female rate, CD proportion, country, and type of risk) on the outcomes.
Conclusions: The VTE incidence in Asian patients with IBD was higher than that in non-IBD patients (RR 2.065). It seemed reasonable to consider prophylaxis for VTE in hospitalized patients with IBD.
PP-02-160
CHIA deficiency in the chief cells promotes SPEM through pyroptosis mediated M2 Macrophage polarization
1Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China;2Department of general surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Loss of parietal cells causes the development of spasmolytic polypeptide-expressing metaplasia (SPEM) through transdifferentiation of chief cells. However, whether initial chief cell loss cause SPEM needs to be further investigated. CHIA is highly expressed in the chief cells, and has an important role in gastric mucosal immune homeostasis. However, the molecular role and mechanism of CHIA in the development of SPEM is need further investigated.
Methods: single-cell sequencing (scRNA-seq) technology, histopathological and immunofluorescence (IF).
Results: PCR and scRNA-seq technology data showed that increased M2 macrophage infiltration and SPEM formation, but remaining of parietal cells in CHIA KO mice, when compared to CHIA WT mice. Furthermore, deletion of CHIA in mice resulted in release of endoplasmic reticulum stress-triggered, including upregulation of endoplasmic reticulum stress markers, followed with upregulation of pyroptosis-related marker, as well as significantly increasement of IL33/IL-13 release. Moreover, Double immunoflorescence staining showed that upregulation and colocalization of M2 macrophage polarization markers, including F4/80 and CD163+ in the gastric mucosal of CHIA KO mice. Notably, IF showed that CHIA deficiency caused the mucous neck cell marker SOX9 shifted from the cervical isthmus into the SPEM cells, and co-localized with the SPEM markers CD44v9, TFF2 and MUC6, suggesting that the origin of SPEM cells may transdifferentiate from mucous neck cell after CHIA gene deletion.
Conclusions: CHIA deficiency in the chief cells promotes SPEM through the pyroptosis mediated M2 macrophage polarization. The origin of CHIA deficiency promotes injury may origin from the transdifferentiation of mucous neck mucus cells into SPEM cells.
PP-02-162
The nutritional analysis of South Korean infants from birth to 2 years
Yeji Ksim1, Youn Ha Kang1, Sun Woo Park1, Jeong Eun Ahn1, Lia Kim1, Homin Huh1, Jong Woo Hahn2, Minsoo Shin3, Jin Gyu Lim1, Ki Soo Kang4, Yeoun Joo Lee5, Jee Yoon Park8, Chan-Wook Park8, Joo Young Kim9, Su Jin Jeong10, Young Hwa Jung2, Jae Hyun Kim2, Chang Won Choi2, Ju Whi Kim1, Seung Han Shin1, Yun Jeong Lee1, Young Ah Lee1, Choong-Ho Shin1, Seung-sik Hwang11, M.S Young Eun Kim1, Jae Sung Ko1 and Jin Soo Moon1
1Department of Pediatrics, Seoul National University College of Medicine, Seoul, South Korea;2Department of Pediatrics, Seoul National University Bundang Hospital, Bundang, South Korea;3Department of Pediatrics, Korea National University College of Medicine, Seoul, South Korea;4Department of Pediatrics, Jeju National University College of Medicine, Jeju, South Korea;5Department of Pediatrics, Pusan National University School of Medicine, Pusan, South Korea;6Department of Pediatrics, Soonchunhyang University College of Medicine, Seoul, South Korea;7Department of Pediatrics, Korea University Guro Hospital, Seoul, South Korea;8Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea;9Department of Pediatrics, Eulji University College of Medicine, Seoul, South Korea;10Department of Pediatrics, CHA Bundang Medical Center, School of Medicine, CHA University, Bundang, South Korea;11Department of Public Health Science, Graduate School of Public Health, Seoul National University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: From a prospective national South Korean children's growth cohort, the nutritional analysis of healthy South Korean infants whose growth parameters lay between the two standard deviation z score for height-for-age, weight-for-age, BMI-for-age were analyzed.
Materials and Methods: A longitudinal study of Korea infant physical growth experimental survey (KIPGros) (n=256) was taken and children aged <24 mo (n= 72) was evaluated. The mean value of growth parameters of each group: younger infants (4 mo – 5.9mo), older infants (6-11.9 mo), toddlers (12-23.9) were calculated. Only the growth parameter which laid within the two standard deviations of the WHO z score of height, weight, and BMI were selected. The Nutritional intake was analyzed and calculated for calorie, protein, fat and carbohydrate by a clinical dietitian, using a Computer Aided Nutritional Analysis program.
Results: Out of the total enrolled number n=256, a smaller subset of 72 children were analyzed. Height-weight-BMI values that lay beyond the two standard deviations were eliminated. The mean height-weight-BMI of younger infants (4mo – 5.9mo) were 64.8cm-7.5kg-17.8m2/kg, older infants (6mo -11.9mo) 70.8cm-8.8kg-17.6m2/kg, toddlers (12mo - 23.9mo) 80.3cm-10.6kg-16.2m2/kg. The mean calorie, protein, fat, and carbohydrate were as follows: younger infants (4mo – 5.9mo) 511.5kcal, 13.0g, 23.4g, 61.3g, older infants (6mo -11.9mo) 532.5kcal, 18.4g, 18.8g, 71.4g, toddlers (12mo -23.9mo) 992.7kcal, 41.7g, 30.7g and 136g.
Conclusion: This was a prospective cohort study of the growth and nutritional analysis of Healthy Korean infants from 0 to 24 months. We hope our outcome will serve as a future growth reference and dietary guidance.
PP-02-163
Burden of cancer incidence/mortality in the China, 1980-2021: a analysis for the GBD 2021
Rong Lin
Huazhong University of Science and Technology, Wuhan, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: China has gone through changes in its social and economic sectors. Thus, efforts need to include thorough evaluations of cancer burden in China.
Materials and Methods: Using data from GBD 2021, we first provide estimates of the incidence, mortality and the contribution of risk factors from 1990/1980 to 2021 in China.
Results: In 2021, neoplasms accounted for 24.07% (95% uncertainty interval 22.74 to 25.29) of total deaths and 17.7% (15.21 to 20.2) of total DALYs in both sexes in the China. In the same period, age-standardized incidence rate (ASIR) and age-standardized deaths rate (ASDR) was 790.17 (676.83 to 926.32) and 137.48 (115.11 to 163.38), respectively. In both sexes in 2021, tracheal, bronchus, and lung cancer showed the highest ASIR (44.01), followed by non-melanoma skin cancer (37.54), colon and rectum cancer (31.44), stomach cancer (29.05), breast cancer (19.36), esophageal cancer (15.04), and liver cancer (9.52). In a word, the ASDR of neoplasms decreased about 29.78% in males and 42% in females in the past forty years in China. Lung cancer showed the highest ASIR (62.63) and ASDR (56.45) in males. Of note, 31.73% of all cancer cases death in China were digestive cancers in 2021. In level 1 risks in 2021, behavioral risks was linked to 73.57% of neoplasm in the all age group.
PP-02-164
Burden of digestive disease among adolescents in China, 1990-2021: a analysis of the GBD 2021
Rong Lin
Huazhong University of Science and Technology, Wuhan, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: There is limited data on the prevalence and risk factors of digestive diseases among adolescents and young adults in the country.
Materials and Methods: Using data from GBD 2021, we first provide estimates of the prevalence and DALYs burden of digestive disease from 1990 to 2021 in the childhood and adolescence.
Results: In 2021, the reported mean prevalence of digestive disease in the age 10 to 24 years group in China was 11.05% (95% UI: 9.81%-12.41%) and accounting for 233,736 (95% UI: 182,862-294,871) DALYs of a total of 10,374,141 DALYs for both sexes. Upper digestive system diseases contributed to approximately 1/3rd (27.4%) of total DALYs followed by gallbladder and biliary diseases (23.3%), cirrhosis and other chronic liver diseases (18.2%), appendicitis (7.5%), inguinal, femoral, and abdominal hernia (7.2%), paralytic ileus and intestinal obstruction (6.5%), other digestive diseases (4.1%), pancreatitis (4%), IBD (1.7%), and vascular intestinal disorders (0.2%). There was some variation in age-related sex-specific patterns. Males had slightly higher overall prevalence and DALYs attributed to digestive disease as compared to females in the 10-24 age group, especially in 20-24 age group. While females had slightly higher overall prevalence (1.41%, 95% UI:1.07%-1.86%) and DALYs 33,609 (95% UI:21,185-50,616) attributed to gallbladder and biliary diseases than male prevalence (0.78%, 95% UI:0.59%-1.04%) and DALYs 20,868 (95% UI:13,132-31,728).
Conclusion: The prevalence and DALYs has been decreasing in children and adolescents of digestive disease in China. Differentiated intervention and outreach strategies based on age and gender would be necessary to reduce the impact of digestive disease.
PP-02-165
The Hidden Culprit: Lead Poisoning in Anemia and Abdominal Pain
Fei Yang Pan1,2 and Brandon Baraty1,2
1Macquarie University Hospital, Sydney, Australia;2Macquarie University, Sydney, Australia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Lead poisoning is rare in developed countries but can occur with traditional medicines or environmental exposures. Acute lead exposure often presents with neurological symptoms and abdominal pain. Hemolytic anemia is a rare complication.
Case Description: A 42-year-old female with no significant medical history presented to the ED on June 4, 2024, with abdominal pain, nausea, chest tightness, and acute difficulty breathing. The pain was severe, fluctuating, and troubling at night. She had been taking traditional Siddha medicines in South India from January to April 2024, stopping just before admission in June.
Her hemoglobin level was 84 g/L, with normal bilirubin, lactate dehydrogenase (LDH) levels, and haptoglobin. Extensive investigations for severe abdominal pain and anemia, including a CT scan, gastroscopy, and colonoscopy, were normal. She required three RBC transfusions. Further investigation with a video capsule endoscopy showed terminal ileum erosions, and a labelled red cell scan indicated a likely bleed in the descending colon. Repeat gastroscopy and colonoscopy were normal.
Multiple blood films did not show basophilic stippling, but a repeat film on July 9 did. Haptoglobin was positive for hemolysis on July 3, with elevated bilirubin and LDH levels. A heavy metal screen revealed elevated lead levels (44.8 μg/dL). Her herbal medications contained lead levels of 13,522.35 μg/g.
Discussion: This case shows that basophilic stippling and a positive hemolytic screen may not always be present in acute lead poisoning with anemia. Clinical suspicion must be high, especially in patients using traditional medicines, which can be a significant lead source.
PP-02-166
Recurrent Gastrointestinal Bleeding Caused by Small Bowel Hemangiolymphangioma: A Rare Case
Mita Hafsah Saraswati1 and Virly Muzellina2
1Universitas Indonesia Hospital/ Dr. Cipto Mangunkusumo National Central General Hospital, Depok/ Jakarta, Indonesia;2Dr. Cipto Mangunkusumo National Central General Hospital, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Hemangiolymphangioma or Hemolymphangioma is rare malformation composed of both lymphatic and vascular vessels. Small bowel hemangiolymphangiomas are extremely rare, with less than 1% of all lymphangiomas. To our knowledge, only 10 small bowel hemangiolymphangioma cases have been reported. Here, we report a hemangiolymphangioma case, which present with gastrointestinal bleeding and anemia.
Case Description: A 34-year-old female complained of recurrent episodes of melena and easily fatigue for more than 2 months. On physical examination, anemic conjunctiva and upper abdominal tenderness were noted. Laboratory analysis revealed severe anemia with hemoglobin of 6.3 g/dL and fecal occult blood was positive. Esophagogastroduodenoscopy showed antral gastritis and colonoscopy result was normal. An anterograde balloon- assisted enteroscopy was performed, and a protruded mass was revealed in the proximal jejunum. Histology from endoscopic biopsies confirmed the diagnosis of hemangiolymphangioma. Later, the patient underwent partial resection of the small intestine. At the 6 months follow-up, no recurrence of melena or anemia was observed.
Discussion: Hemangiolymphangioma is an uncommon vascular malformation characterized by the proliferation or network formation of vascular spaces, which can include lymphatics, capillaries, veins, or arteries. It is rare among adults. Hemangiolymphangioma often difficult to diagnose due to their location and the limited visibility of conventional endoscopy. However, advances in diagnostic tools such as balloon-assisted enteroscopy have improved the detection of these malformations. This case highlights the importance of considering hemangiolymphangioma as a cause of recurrent gastrointestinal bleeding.
PP-02-167
Gastrointestinal (GI) Bleeding With Anti-thrombotic Use: A Large Malaysian Cohort Study
1Gastroenterology Unit, Department of Internal Medicine, University Malaya Medical Centre, Kuala Lumpur, Malaysia;2Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: There have been limited reports studying GI bleeding due to anti-thrombotic use in a diverse, large population, especially in South East Asia. This study aims to assess the relation of anti-thrombotics with GI bleeding; to describe its demographics, clinical characteristics and endoscopic outcomes.
Materials and Methodology: This study was a retrospective audit in University Malaya Medical Centre (UMMC). All patients referred for suspected GI bleeding and underwent GI endoscopy (gastroscopy and colonoscopy) from 1/1/2013 to 31/12/2021 were included. Patients were identified from manual endoscopy logbooks. Demographic and relevant information including endoscopic outcomes were charted via the hospital’s electronic medical records (EMR) database.
Results: There were 5222 patients referred for GI endoscopy due to suspected GI bleeding. Peptic ulcer was the main cause in the upper tract (28.3%) and hemorrhoids, in the lower tract (17.4%). 1548 patients (29.6%) were on anti-thrombotics; 337 patients (6.5%) were on anticoagulants and 1211 patients (23.2%) were on antiplatelets. The most common anticoagulant use that presented with GI bleeding were warfarin (33%), apixaban (28.9%) and dabigatran (18%). Antiplatelets that presented with GI bleeding were mainly aspirin (12.9%) and clopidogrel (3.8%). Use of warfarin, apixaban and rivaroxaban were significant risks for GI bleeding (p<0.001).Age, presence of comorbidities such as hypertension, chronic kidney disease, ischaemic heart disease and atrial fibrillation; and aspirin use were also found to be significant risk factors for GI bleeding. (p<0.001)
Conclusion: Significant risk factors for GI bleeding were age, comorbidities , antiplatelet use and usage of warfarin, apixaban and rivaroxaban.
PP-02-168
Small intestinal arteriovenous malformation presenting as recurrent obscure, overt gastrointestinal bleeding in a 34-year-old male
Jonathan Viernes, Juvaline Valete and Caesar Noel Palaganas
Baguio General Hospital and Medical Center, Baguio City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Small intestinal bleeding poses a diagnostic challenge. Vascular lesions are common lesions of the small intestine. However, arteriovenous malformations in the small intestines are rare. They are commonly located in the cecum and right side of the colon.
Case report: Patient V. G., a 34-year-old male, Filipino, was admitted due to recurrent episodes of hematochezia which started about 9 months prior to admission. Upper and Lower GI endoscopy done at another local hospital 4 months prior to admission only revealed non-specific colitis and erosive gastritis. Repeat endoscopy was done at our institution and revealed Non-erosive Gastritis and blood clots were seen from the cecum to the rectum. Due to persistent hematochezia, mesenteric angiogram was done revealing multiple tortuous dilated vessels tangled in the left hemiabdomen draining some segments of the jejunum. On exploratory laparotomy, arteriovenous malformations were seen in the jejunum, 30 centimeters from the ligament of Treitz, with involved segment of 50 centimeters. Intraoperative enteroscopy revealed multiple dilated vessels with oozing blood. Segmental resection with primary anastomosis was done. Histopathologic examination of the resected segment was consistent with arteriovenous malformation. The patient’s condition improved.
Discussion: Arteriovenous malformation in the small intestines is a rare cause of GI bleeding. Preoperative diagnosis and localization of arteriovenous malformations of the small intestines is important prior to any intervention. In this case report, mesenteric angiogram and intraoperative enteroscopy played a useful role in the diagnosis. Treatment for AV malformations of the small intestines is surgical resection.
PP-02-169
A Systematic Review of Adverse Effects Associated with Proton Pump Inhibitor Long-Term Use
Hayati Alwani Yahaya
National University Of Malaysia, Kuala Lumpur, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Proton pump inhibitors (PPIs) are commonly prescribed for the treatment of acid-related disorders. Prolonged use of PPIs may be associated with several potential adverse effects, raising questions about the risk-benefit balance of extended therapy. This systematic review seeks to address these concerns by evaluating published literature that investigates the long-term adverse effects of PPIs use, aiming to provide a comprehensive understanding of its safety profile.
Methods: We conducted a comprehensive literature search across PubMed, Scopus and Web of Science for observational studies published up to 2024. Our search strategies included controlled vocabulary and related keywords: adverse effects of long-term PPIs use. Two authors independently screened the articles for inclusion, with a third author resolving disagreements. Quality and risk of bias were evaluated using the Newcastle-Ottawa Scale.
Results: A total of 45 studies were included in the review. Prolonged PPIs use was associated with a range of long-term effects. The most common were increased bone fragility, followed by higher susceptibility to enteric infections, possible risk of gastric cancer and impaired micronutrient absorption. The duration associated to adverse effects was 4 weeks with some studies indicating higher risks associated with extended use. Most adverse effects were reported in observational studies with consistent results and significant confounding risks.
Conclusion: Long-term PPIs use is associated to several potential adverse effects, though evidence strength varies. It is crucial to weigh the risks and benefits of extended PPI therapy. A multidisciplinary approach from healthcare providers is needed to provide better recommendations for PPIs prescribing.
PP-02-170
Metabolic syndrome and the risk of colorectal cancer in individuals younger than 50 years
Jung Eun Yoo1,2 and Eun Hyo Jin3,4
1Department of Family Medicine, Seoul National University Hospital Healthcare System Gangnam Center, Seoul, South Korea;2Department of Family Medicine, Seoul National University College of Medicine, Seoul, South Korea;3Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea;4Department of Internal Medicine, Healthcare Research Institute, Seoul National University Hospital Healthcare System Gangnam Center, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Metabolic Syndrome (MetS) is a cluster of diverse conditions that has been linked to an increased risk of early onset colorectal cancer (CRC). However, the impact of evolving MetS status over time on early onset CRC risk remains unknown.
Materials and Methods: Eligible participants from the National Health Insurance Service database in South Korea were followed up till 31 December 2011. In total, 3,340,635 individuals who underwent health examinations between 2009 and 2011 were categorized into four groups based on changes in MetS status: MetS-free (no-no), recovered (yes-no), developed (no-yes), and persistent (yes-yes). CRC risk was assessed for the 20–39 and 40–49 years groups until the end of 2019.
Results: Among the 20–39-year-old’s, those with persistent MetS had a 16% higher risk of CRC, whereas those in the 40–49 years old group had a 22% higher risk, even after adjusting for covariates. The subgroup analysis revealed differences in risk based on sex, alcohol consumption, and diabetes. Persistent MetS was associated with a 34% higher risk of distal colon cancer in the 40–49 years old group. Similarly, in the 20–39 year old’s, it was linked to a 29% higher risk of rectal cancer.
Conclusion: Persistent MetS significantly increases the risk of early onset CRC in both age groups (20–39 and 40–49 years). This underscores the importance of actively managing MetS, particularly in the 20–39 years age group, to mitigate future CRC development. Therefore, tailored screening strategies should be considered for individuals with persistent MetS, even for those aged <50 years.
PP-02-171
Accelerometer-Derived “Weekend Warrior” Physical Activity and Digestive Diseases: a nationwide cohort study
Shiyi Yu1,2,3,4, Jing Feng1,2, Chun Ling Chen1,2, Qinming Li1,2, Weihong Sha1,2 and Hao Chen1,2
1Department Of Gastroenterology, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou, China;2The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China;3HKUMed Laboratory of Cellular Therapeutics, the University of Hong Kong, Hong Kong, SAR, China;4Faculty of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Abstract
Objective: To investigate associations between accelerometer-derived weekend warrior patterns and more evenly spread MVPA with the risk of incident digestive diseases.
Design: Population-based cohort study.
Participants: The study included 89,595 UK Biobank participants with wrist-based accelerometer data.
Exposures: Participants were categorized into three physical activity patterns: active weekend warriors (active WW, achieving 150+ minutes with ≥50% of MVPA on 1-2 days), active regulars (meeting 150+ minutes but not active WW), and inactive (less than 150 minutes). These patterns were also assessed using a sample median threshold of 230.4+ minutes of MVPA per week.
Main outcome measures: Incidence of 16 digestive diseases.
Results: Over a median follow-up of 7.9 years, both active patterns were associated with lower risks of incident digestive diseases compared to the inactive group, at both the guideline-based (≥150 minutes) and median (≥230.4 minutes) thresholds. At the guideline-based threshold (≥150 minutes), multivariable-adjusted hazard ratios (HRs) for weekend warriors and regularly active participants were 0.83 (0.79-0.87) and 0.79 (0.75-0.84) for any digestive disease; 0.67 (0.57-0.80) and 0.63 (0.51-0.78) for NAFLD; 0.89 (0.84-0.95) and 0.84 (0.78-0.90) for diverticulosis; 0.82 (0.76-0.90) and 0.85 (0.77-0.94) for constipation; 0.74 (0.66-0.82) and 0.70 (0.61-0.80) for cholelithiasis; 0.79 (0.74-0.85) and 0.76 (0.70-0.82) for GERD.
Conclusion: Both weekend warriors and regular physical activity participants exhibited lower risks of incident digestive diseases compared to inactive individuals. Physical activity concentrated within 1-2 days was associated with a similarly lower risk of any digestive disease, NAFLD, diverticulosis, constipation, cholelithiasis, and GERD as more evenly distributed activity.
PP-02-172
Relationship of Neutrophil-to-Lymphocyte Ratio and morbidity among patients with cirrhosis admitted in a tertiary hospital
Ameer Hussein Abbas and Johaniya Dimaporo and Abdel Hussein Lucman
Department Of Health, Amai Pakpak Medical Center, Marawi, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This is a baseline study in a tertiary government hospital in Lanao Del Sur, Philippines among patients admitted with cirrhosis. This also studies the relationship between Neutrophil-to-Lymphocyte Ratio (NLR) and morbidity in terms of the length of hospital stay, and in-hospital mortality. NLR is an inexpensive, objective, and widely available biomarker.
Materials and Methods: This study analyzed all available records of patients admitted with cirrhosis from January-2018 to December-2022. Demographic characteristics, etiology, complications, length of stay, and mortality were documented. Prognostic markers namely NLR, CPC, MELD, and FIB4 were computed. NLR of each patient was analyzed in relation to patient outcome using Chi-square and Mann-Whitney test.
Results: A total of 145 patient records were analyzed. Results showed that majority of the patients diagnosed with cirrhosis are men (59%); aged 60 and above (57%); with etiology of NAFLD (30%), schistosomiasis (24%), and hepatitis B (21%); patients sought consult during the onset of complications mostly due to ascites (33%), jaundice (19%), and abdominal pain (17%); and with an early score of CPC and MELD. Majority of the patients were discharged improved. In-hospital mortality rate was 5%. NLR of patients was categorized into ≤ 5, and greater than 5. No statistical significance was seen between NLR and the length of hospital stay (p value= 0.5), and in-hospital mortality (p value= 0.09).
Conclusion: Most of the etiologies of patients admitted with cirrhosis are preventable. No statistical significance was seen between NLR and the length of hospital stay, and in-hospital mortality in this study.
PP-02-173
Serum microRNA signature as biomarkers for prediction of decompensation in patients with cirrhosis
Yingjie Ai, Sitao Ye, Xiaoquan Huang and Shiyao Chen
Zhongshan Hospital, Shanghai, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Cirrhosis is a worldwide health concern and could attribute to different etiologies. Evolvement into decompensation often leads to poor outcomes. Therefore, noninvasive and accurate method for prediction and detection is in urgent need. This study aimed to identify microRNAs(miRNAs) as potential predictive biomarkers.
Materials and Methods: MiRNA sequencing with serum sample from 16 cirrhosis patients was conducted. They were divided into two groups (8CE vs. 8nCE) according to occurrence of decompensated events including variceal bleeding, carcinoma, PVT, ascites and SBP. The expression pattern of candidate miRNAs was further tested in a validation cohort (20CE vs. 20nCE) with RT-PCR assays.
Results: Six differentially expressed miRNAs were identified with 3 different methods among which five were downregulated while one was upregulated. Logistic regression showed that decreased miR195-5p and miR1287-5p were associated with cirrhosis progression (p = 0.03, 0.04). Their change pattern was further confirmed in the validation cohort. These two miRNAs shared 686 downstream target genes which may account for decompensation as they were involved in pathways like Hepatocellular carcinoma, EGFR tyrosine kinase inhibitor resistance, etc. Hub genes including EGFR, PTEN, BDNF, PPARs were also tested by RT-PCR and presented corresponding dysregulation.
Conclusion: MiR195-5p and miR1287-5p could act as a potential biomarker in prediction of decompensation and help provide further personalized treatment to improve outcomes.
PP-02-174
Outcomes of a no prophylactic transfusion strategy in patients with cirrhosis undergoing high-risk invasive procedures
All India Institute Of Medical Sciences, New Delhi, New Delhi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Limited data exists on the outcomes of performing high-risk procedures in patients with cirrhosis and platelet counts <50 x 109/L, without any prophylactic platelet transfusion.
Methods: Data from two prior randomized controlled trials (CTRI/2017/12/010822, and CTRI/2021/05/033464) on high-risk procedures in patients with cirrhosis were combined. We included patients with cirrhosis and severe thrombocytopenia while excluding patients with acute-on-chronic liver failure, renal dysfunction and patients on antiplatelet agents or anticoagulants. Patients were divided into two groups: (i) Standard-of-care: all patients received prophylactic platelet transfusions, and (ii) On-demand: no patients received any prophylactic transfusion. Outcome measures included major bleeding within 5-and 28-days of the procedure, requirement of blood transfusion and bleeding-related mortality.
Results: Fifty patients received prophylactic transfusions while 68 patients did not. Baseline demographics were well matched.
Median Child and MELD score of the cohort was 6 (5-7) and 12 (10-14.3) respectively. Median platelet count was 35.5 (28.0-44.3) x 109/L. Percutaneous liver biopsy was the most common procedure (73 patients, 61.9%). No major procedural bleeding or bleeding-related mortality occurred within 28 days of the procedure. One patient (2%) in the SOC arm and 4 (5.8%) in the on-demand arm had minor (WHO Grade 1) bleeding. Only 5 (7.4%) patients in the on-demand arm received platelet transfusions (17 units) as compared to all 50 patients (150 units) in the SOC arm.
Conclusion: High-risk procedures can be performed safely in a select group of cirrhosis patients without prophylactic platelet transfusions. This would reduce empirical and irrational transfusion practices in these patients.
PP-02-175
The Correlation of Plasma Creatinin to Albumin Ratio for Prognosis and Fibrosis in Cirrhotic Patients
Indro Buono1, Rusdiyana Ekawati1, Titong Sugihartono2 and Budi Widodo2
1Internal Medicine Subspeciality (Sp-II) Study Program, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga- Dr. Soetomo General Academic Hospital, Surabaya, Indonesia;2Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Renal impairment is a common complication of cirrhosis, reported in up to one-third of hospitalized patients with advanced liver disease. Albumin is exclusively synthesized by hepatocytes, so that decrease in liver cirrhosis. Liver fibrosis is an important predictor of progression and prognosis in cirrhosis. Plasma Creatinin-Albumin ratio (CAR) can be proposed as a simple, affordable, and easily accessible marker to predict prognosis in liver cirrhosis. The aim of this study to investigate the relationship between plasma CAR and prognosis by Child Pough (CP) also between plasma CAR and fibrosis by APRI and FIB-4 score in cirrhotic patients.
Material and method: We performed a single-center, observational retrospective study and analyzed to 217 cirrhotic patients were admitted in the gastroenterohepatology department. The plasma CAR of patients was calculated from blood samples taken at the same time as the scoring examination. The biochemical examination results of three scores (Child Pough, APRI, and FIB-4) were calculated. Correlation between plasma CAR to both scores has been analyzed.
Results: Spearman correlation analysis showed correlation indicated that there were statistically significant positive relationship between plasma CAR to CP score (r= 0.642, p < 0.001), APRI score (r = 0.776, p <0.01), and FIB-4 score (r=0.674, p= 0.006).
Conclusion: Plasma Creatinin to Albumin Rasio can be a useful biomarker used for evaluating the prognosis and fibrosis in cirrhotic patients. Further researches are warranted to confirm correlation between Plasma CAR and other invasive or noninvasive liver fibrosis examinations.
PP-02-176
Bacteriological Profile And Antibiotic Resistance Patterns In Adult Cirrhotic Patients With SBP
Sobin Chaithram Bhaskaran1, Ramesh Mohanachandran Nair2, K George Thomas3, Mercy John Idikkula3 and U B Thushara4
1St. Thomas Hospital, Chethipuzha, Chanaganassery, Kottayam, India;2Mar Sleeva Medicity, Pala, Kottayam, India;3Pushpagiri institute of medical sciences and research centre, Thiruvalla, Pathanamthitta, India;4Believers Church medical college hospital, Thiruvalla, Pathanamthitta, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The objective of study was to evaluate the bacteriological profile and Drug resistance patterns of SBP pathogens.
Materials and methods: A retrospective study was done for a period of 1 year in a tertiary care hospital in Kerala. A total of 32 adult cirrhotic patients, meeting the inclusion and exclusion criteria were grouped into community acquired and nosocomial based on timing of diagnosis of SBP(within 48 hours and >48 hours) and underwent ascitic fluid paracentesis and sample was sent for culture and sensitivity. Data thus obtained was grouped and studied using Chi Square test and analysis was done using IBM-SPSS.
Results: Out of 32 cases, Gram negative bacteria were more frequent than gram positive bacteria(78.1% vs 21.8%) and 9(28.1%) were classified as multi drug resistant(MDR) bacteria. E.coli was the predominant bacteria(18, 56.2%) of which 5(27.7%) was found to be MDR. Resistance to third generation cephalosporins, quinolones, piperacillin tazobactam and carbapenems were found in 53%, 62.5%,28.1% and 21.8% of cases respectively. MDR bacteria and gram positive bacteria were more common in nosocomial group(45.5%, 36.4%, p = 0.03). Except for Third generation cephalosporins(45.5% vs.57.1%), resistance to quinolones(63.6% vs 61.9%), piperacillin tazobactam(45.5% vs 19%) and carbapenems(36.4% vs 14.3%) were more frequently found in nosocomial group.
Conclusions: One third of the study population were found to have MDR. Resistance to third generation cephalosporins and quinolones, which is used for empirical antibiotic treatment and prophylaxis of SBP was high. Hence antibiotic use according to local antibiogram should be strictly followed.
PP-02-177
Utilization of systemic inflammatory markers as non-invasive prognostic markers for mortality in liver cirrhosis
Ma. Althea Kathrine Elinzano
East Avenue Medical Center, Quezon City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Cirrhosis-associated immune dysfunction encompasses a specific range of immune changes that occur in the context of chronic liver disease. This study aims to determine if CAR, SII, NLR, MLR, and PLR can predict mortality in liver cirrhosis.
Materials and methods: This is a single-center, retrospective cohort study which included 219 Filipino patients admitted with liver cirrhosis. Descriptive statistics was used to summarize the demographic, biochemical, and clinical characteristics. Area under the receiver operating characteristic curve was used to determine the diagnostic performance of the systemic inflammatory markers to discriminate mortality.
Results: The CTP, MELD, MELD-Na scores, and systemic inflammatory markers (NLR, MLR, PLR, CAR, SII) were significantly different between the expired and alive groups. The most common etiology for liver cirrhosis was chronic hepatitis B (44.29%), followed by alcoholic liver disease (39.27%). Majority of the expired patients had decompensated liver cirrhosis (81.36%). The leading cause of mortality among the expired group was hepatic encephalopathy (52.64%).
Conclusion: Overall, systemic inflammatory markers (NLR, MLR, CAR, SII) alone are insufficient to predict mortality. Their combination with established liver scoring systems (NLR-CTP, NLR-MELD, NLR-MELD-Na, MLR-CTP, MLR-MELD, MLR-MELD-Na, CAR-CTP, CAR-MELD, and CAR-MELD-Na scores) may offer additional prognosticating value, highlighting the potential for improved risk stratification and patient management. Among these markers, the CAR-CTP score with a cutoff level of > 12.24 had the highest accuracy (70.78%), sensitivity (71.19%), and specificity (70.30%) in predicting mortality in liver cirrhosis.
PP-02-178
Dietary Pattern and Energy Fulfllment among Cirrhosis Patient with Hepatic Encephalopathy and Non Hepatic Encephalopathy
Mochamad Anief Ferdianto1, Juferdy Kurniawan1 and Hasan Maulahela2
1Hepatology Division, Department of Intenal Medicine, Dr Cipto Mangunkusumo National Referral Hospital/Faculty of Medicine Universitas Indonesia, Jakarta Pusat, Indonesia;2Gastroenterology Division, Department of Intenal Medicine, Dr Cipto Mangunkusumo National Referral Hospital/Faculty of Medicine Universitas Indonesia, Jakarta Pusat, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We would like to investigate both descriptively and analytically the difference of dietary pattern and energy fulfilment among cirrhosis patient with and without hepatic encephalopathy (HE).
Material and Methods: We conducted a cross-sectional study in Hepatology Polyclinic of dr Cipto Mangunkusumo National Referral Hospital, Jakarta during whole 2023 period. Hepatic encephalopathy diagnosis were made clinically with adjuvant Stroop and Flicker tests. Dietary pattern including carbohydrate, protein, fat, and dietary fiber was determined using 24 hours food recall questionnaire (Indonesia language validated) which the results calculated for energy fulfillment. We compared the median of both groups using Mann-Whitney test to elucidate the difference.
Results: 86 patients (66 non HE and 20 HE) enrolled in this study. Study subjects mean age was 53 ± 8,10 years with no difference between BMI level (p=0,713) and obesity prevalence in both groups (p=0,801). Energy, protein, fat, carbohydrate, and fiber consumption was found not to be different between HE and non HE patients. (p>0,05). However energy (p=0,003) and carbohydrate (p=0,036) daily requirement fulfillment was found higher in HE compared to Non-HE significantly.
Conclusions: Absolute consumption of dietary component did not differ between groups. Daily energy and carbohydrate requirement fulfillment was discovered higher in HE group.
PP-02-179
Simvastatin in Treatment and Reducing Events of Decompensation in Patients With Liver Cirrhosis: A Meta-Analysis
Nicole Ferrer, Miguel Jemuel Cabusas and Ian Homer Cua
St. Luke's Medical Center Bonifacio Global City, Taguig City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To assess the efficacy and use of Simvastatin (Statin) in treatment and reducing events of decompensation in patients with decompensated Liver Cirrhosis. To investigate the effect of Simvastatin in improving liver function. To determine if simvastatin treatment leads to a reduction in portal hypertension, and
subsequent reduction in events of decompensation. To analyze the effect on patient’s clinical outcomes
Materials and Methods: A Meta-analysis was done using randomized control trials who applied patients aged 18-75 years old with known liver cirrhosis with portal hypertension using Simvastatin as therapy. Decrease in levels of HVPG (Hepatic Venous Pressure Gradient) or PHI (Portal Hypertension Index) post treatment was used as marker for improvement.
Results: There was significant difference in HVPG levels and liver function among patients with liver cirrhosis who had adjunct use of simvastatin vs. standard treatment. Visual inspection of the forest plot appears to favor use of simvastatin in improving liver function in patients with liver cirrhosis. A moderate heterogeneity was also observed using the fixed effect model (Chi2 = 4.87, P = 0.08, I2 = 38%).
Conclusion: While earlier data depict statins to have harmful effects in the liver, evidence, as shown in this paper, suggests that there is positive impact in reducing events of decompensation & portal hypertension. Combining this knowledge and the long track-record of safety and tolerability of statins, we might soon rely on statins to achieve better outcomes in patients with liver cirrhosis without significant additional costs.
Division of gastroenterology, Jichi Medical University, Shimotsuke, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Patients with advanced chronic liver disease (CLD) such as cirrhosis often have thrombocytopenia associated with increased splenic function. On the other hand, patients with CLD, including hepatocellular carcinoma may require invasive procedures with the risk of bleeding for examinations and treatments. In these cases, platelet transfusion is sometimes required to reduce the risk of bleeding complications. In 2015, the administration of lusutrombopag became available, and we have experienced cases in which platelet transfusion could be avoided. Avatrombopag, which was newly approved in 2023, is no need to check platelet counts 5 days after administration, so it is considered more useful in clinical setting. In this study, we examined the efficacy and safety of avatrombopag.
Methods: Eight patients with CLD who received avatrombopag between June 2023 and June 2024 were retrospectively evaluated for serological parameters, Spleen Index, and platelet increase.
Results: The median Spleen Index was 92.0 cm² (22.1-112.5) and the median platelet count before administration of avatrombopag was 4.9×10³/μL (3.6-5.0). All patients showed an increase in platelets, and platelet counts increased to over 5.0 ×10³/μL, therefore, platelet transfusion avoidance rate was 100%, and no hemorrhagic event occurred.
Conclusions: Avatrombopag was useful in safely and effectively increasing platelet counts in patients with CLD and thrombocytopenia who were scheduled to undergo invasive procedures. In addition, there is no need to check platelet counts after the administration of avatrombopag, so it is considered to be beneficial in clinical practice use.
PP-02-181
Clinical and Paraclinical Features of Decompensated Cirrhosis during COVID-19 Pandemic: A One-Year Single-Center Study.
Lyly Heng1, Channa Sann1,2, Ong Chea1,3, Vutha Ky1,2, Sopheak Mak1,2, Sidet Chheang1,2 and Veasna Moeung1
1University Of Health Sciences, Phnom Penh, Cambodia;2Department of Gastroenterology and Hepatology, Calmette Hospital, Phnom Penh, Cambodia;3Cambodia-China Friendship Kossamak Hospital, Phnom Penh, Cambodia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: Decompensated cirrhosis, an advanced stage of chronic liver disease, leads to high morbidity and mortality with various complications and significantly reduced life expectancy. This study aims to illustrate and characterize the clinical and paraclinical features of various etiologies of decompensated cirrhosis and its complications, while concurrently assessing any additional comorbidities to optimize diagnostic and management strategies.
Methods: This retrospective, analytical, single-center study was conducted in the Department of Gastroenterology and Hepatology at Calmette Hospital, involving patients with decompensated cirrhosis over a 12-month period.
Results: Out of a total 200 decompensated cirrhosis, the mean age of the patients was 58.44 ± 12.219 years, with a male predominance of 65.5% and the six ratio (M/F) of 1.89/1. Among these patients, 29% were from Phnom Penh, while the remaining 71% were from the provinces. The primary reasons for hospitalization included GI bleeding (45%), ascites (23.5%), confusion (8.5%), and other causes (23%). The most common etiologies of cirrhosis were HBV (30%), alcoholism (27%), HCV (25%), HBV-HCV co-infection (5.5%), and other causes (12.5%). During hospitalization, the most frequently observed complications were GI bleeding (77%), ascites without SBP (75%), ascites with SBP (3.5%), HCC (17.5%), and hepatic encephalopathy (13%).
Conclusion: This study highlights the significant impact of decompensated cirrhosis, primarily in middle-aged males, with major causes being hepatitis B and alcoholism. Key issues include gastrointestinal bleeding and ascites, with severe complications common. The findings emphasize the need for targeted strategies to improve diagnosis, management, and patient outcomes.
PP-02-182
Gut Metagenomic Characteristics of Cirrhotic Patients with Gastrointestinal Bleeding
教授 Hui Huan and 副教授超刘
西藏自治区人民政府驻成都办事处医院, 成都, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Investigating changes in gut microbiota may aid in developing predictive markers and treatments for Gastrointestinal bleeding (GIB)
Methods: This study included 26 Tibetan cirrhotic patients, divided into two groups: 13 cirrhotic patients with GIB and 13 cirrhotic patients without GIB. Gut microbiota profiles were analyzed using metagenomic sequencing.
Results: The gut microbiota composition differed significantly between the two groups. At the genus level, the abundance of Enterobacter, Enterococcus, Lactobacillus, and Clostridium was significantly higher in patients with GIB, while Butyricicoccus, Akkermansia, Bacteroides, and Faecalibacterium were significantly lower. The abundance of Firmicutes showed significant changes, with higher levels of Ruminococcus gnavus, Tyzzerella nexilis, Erysipelatoclostridium ramosum and Bacteroides sp._3_2_5 observed in patients with GIB compared to those without GIB. The beta diversity was significantly lower in patients with GIB (p<0.001). Functionally, the abundance of the ABC-type multidrug transport system, ATPase component, signal transduction histidine kinase, acetolactate synthase large subunit were increased in the GIB group. KEGG pathways such as non-homologous end joining, AGE-RAGE signaling pathway in diabetic complications, hematopoietic cell lineage, cytokine-cytokine receptor interaction, and NF-κB signaling pathway were enriched in the GIB group, while glycolysis/gluconeogenesis, glycine, serine and threonine metabolism, D-alanine metabolism, and epithelial cell signaling in Helicobacter pylori infection were decreased. Spearman correlation analysis showed that hepatic venous pressure gradient was positively correlated with the abundance of Ruminococcus gnavus.
Conclusion: Cirrhotic patients with GIB exhibit reduced gut microbiota diversity and altered microbial composition. The significantly changed bacterial species may serve as predictive markers or therapeutic targets for gastrointestinal bleeding.
PP-02-183
Hepatic sarcoidosis- an unusual cause of jaundice: a case report
Md. Musab Khalil
Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Sarcoidosis is a chronic disorder. Its hallmark is the presence of noncaseating granuloma. Most cases are asymptomatic. However, few of them progress to liver cirrhosis and liver failure. Treatment of hepatic sarcoidosis is challenging as there is no large randomized controlled till date has been done to evaluate the efficacy of drugs. The Unique characteristic of this case report is that Sarcoidosis may present with jaundice and need to be borne in mind when a patient presenting with jaundice and no usual cause failed to be identify any usual cause. Despite having sarcoidosis, the patient did not have any respiratory symptom.
Case presentation: We are presenting a case of hepatic sarcoidosis who presented with abdominal pain, fever and jaundice. After excluding of all common causes of jaundice, hepatic sarcoidosis was diagnosed with liver biopsy.
Conclusion: High levels of suspicion is needed to diagnose hepatic sarcoidosis as this is an unusual cause of jaundice. Although rare, hepatic sarcoidosis can be treated with steroids. The prime take-away message from this case report is to consider Sarcoidosis as an unusual but treatable cause of jaundice when the conventional causes are failed to be the cause of jaundice.
PP-02-184
Hepatic multiphase CT with Low-concentration Iodine Contrast (270mg Iodine/mL) using Low Mono-energetic Image
Jin Sil Kim
Ewha Womans University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to evaluate whether the image quality and detectability of hepatic multiphase CT with low-concentration iodine contrast(270mg Iodine/mL) using a low mono-energetic image(40keV) (LCLM CT) are non-inferior to those of standard CT (high-concentration iodine contrast, 350mg Iodine/mL) using hybrid iterative reconstruction(SDCT) in patients with chronic liver disease focusing on arterial phase
Materials and Methods: This study included 67 patients with LCLM CT and SDCT. Qualitative and quantitative image noise and contrast were also compared between 2 different CT images. Lesion conspicuity was analyzed using generalized estimating equation analysis, and the detectability of hepatic arterial focal lesions was compared using the Jackknife free-response receiver operating characteristic analysis.
Results: Mean overall arterial phase image quality scores with LCLM CT and SDCT were 4.90 ± 0.39 and 4.94 ± 0.24, showing noninferiority (difference: -0.045; 95 %CI, -0.123 to 0.034). Qualitative noise, contrast, and hepatic artery clarity of the two CT images were also not significantly different(p >0.05). Contrast-to-noise ratio of the aorta was higher in LCLMCT than in SDCT (71.8 ± 12.6 vs 46.6 ± 14.9 p <0.0001 ). The comparative analysis demonstrated that lesion conspicuity was slightly higher on SDCT than on LCLM CT images without statistical significance (P= 0.195). The figure of merit for detectability of arterial hepatic focal lesion was 23 for LCLMCT and 22 for SDCT, showing noninferiority (difference: - 0.0217, 95 %CI: –0.0666 to 0.0232).
Conclusion: LCLM CT showed non-inferior overall image quality and detectability of arterial focal hepatic lesions compared to SDCT
PP-02-185
The Impact of Fecal Bifidobacterium/Enterobacteriaceae Ratio on 90 days Hospitalization and Mortality in Liver Cirrhosis
Cokorde Istri Yuliandari Krisnawardani K1, Komang Agus Wira Nugraha2, Ketut Mariadi2 and Gde Somayana2
1Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Denpasar, Indonesia;2Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: The imbalance of gut microbiota in liver cirrhosis patients, particularly a decreased Bifidobacterium and Enterobacteriaceae (BE) ratio, has been linked to potentially worsening liver inflammation and related complications. The specific impact of changes in this ratio on short-term outcomes such as 90-day mortality and hospitalization in liver cirrhosis patients is unclear. This study aimed to explore the relationship between fecal BE ratio and these critical outcomes.
Materials and Methods: This single-center prospective cohort study involved liver cirrhosis patients. Individuals with recent gastrointestinal bleeding, use of antibiotics, systemic infection, or gastrointestinal infection were excluded. Fecal Bifidobacterium and Enterobacteriaceae (CFU/g) were examined using DNA isolation, and BE ratios were calculated. The ratio was categorized as decreased (<0.0184 CFU/g) or normal-increased (≥0.0184 CFU/g). Subjects were followed for 90 days, and hospitalization and mortality were recorded.
Results: Eighty patients (70% male) with a median age of 54 years were enrolled. The cumulative 90-day hospitalization and mortality rates were 30% and 17.5%, respectively. The Kaplan-Meier curve revealed no significant difference in mean survival time between subjects with decreased and normal-increased BE ratios (p=0.595). In Cox Regression analysis, a decreased BE ratio was not associated with hospitalization (HR: 1.160, 95% CI 0.512-2.630, p=0.722) or mortality (HR: 1.096, 95% CI 0.378-3.180, p=0.866), but worse liver function (Child-Pugh score ≥7) was associated with hospitalization (HR: 3.648, 95% CI 1.368-9.950, p=0.010) and mortality (HR: 5.289, 95% CI 1.173-23.83, p=0.030).
Conclusion: In this study, decreased BE ratios were not associated with 90-day hospitalization and mortality in liver cirrhosis patients.
PP-02-186
Serum fibrinogen vs INR in predicting rebleed and mortality in cirrhotic patients
Rishikesh Malokar, Sanjay Chandnani, Shubham Jain and Pravin Rathi
Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: Predicting and managing bleeding in cirrhotic patients has remained challenging.
No single parameter can accurately predict future bleeding risk and prognosis. The aim of the study is to evaluate the role of fibrinogen level and INR in patients with cirrhosis with bleeding to predict rebleeding risk, hospitalization, and mortality.
Method: A prospective observational study. Interim analysis of 84 cirrhotic patients who presented with bleeding. Assessment of association of low fibrinogen (<120mg/dl) and high INR (>1.5) with the first bleeding episode, rebleeding, admission, and mortality. Patients were followed up for 1 year.
Results: Out of 84 patients,16 had low fibrinogen (<120mg/dl), and 28 patients had an INR >1.5. In the low fibrinogen group(N=16), 9 (56.25%) had rebleeding,8 (50%) had repeated admission, and 7 (43.75%) patients died. In the high INR group (N=28), 12(42.85%) had rebleeding, 11(39.28%) needed repeated hospitalization, and 13(46.42%) died. The difference between both groups for rebleeding episodes (p =0.09), repeated admissions (p=0.11), and mortality (p=0.91) at 1 year was not statistically significant.
Conclusion: There is no difference between low fibrinogen and raised INR as a predictor of rebleeding episodes and future hospitalization and mortality in patients with cirrhosis. In this interim analysis, although the p-value of rebleeding rates was insignificant, there is a trend in numbers supporting low fibrinogen as a risk factor.
PP-02-187
The Value Of Albi And Albi-Plt Score In Predicting Esophageal Varices In Cirrhotic Patients
Thang Nguyen Dinh
Cho Ray Hospital, Ho Chi Minh City, Viet Nam
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to describe the prevalence of esophageal varices (EVs) in liver cirrhosis patients and to evaluate the accuracy of the ALBI and ALBI-PLT scores in predicting the presence of EVs in these patients.
Materials and Methods: We conducted a cross-sectional study that enrolled 152 cirrhotic patients from 11/2022 to 5/2023 at the Gastroenterology Department of Cho Ray Hospital.
Results: The study population consisted of 152 cirrhosis patients, with 100 males and 52 females, ranging in age from 28 to 87 years (mean: 56 years). The prevalence of EVs in cirrhotic patients is 56%. The ALBI score has moderate predictive ability for the risk of having EVs (AUC = 0.78), as well as for the risk of varices needing treatment (VNT) (AUC = 0.79). The ALBI-PLT score also demonstrates moderate predictive ability for the risk of having EVs (AUC = 0.76) and VNT (AUC = 0.77). The cutoff threshold for the ALBI score in predicting EVs in cirrhotic patients is -2.76, with sensitivities and specificities being 96.5% and 90.3%, respectively. An ALBI score below -2.76 would have spared 20.4% of patients from EGDs, missing 9.7% of VNT. For the ALBI-PLT score, the cutoff threshold in predicting EV in cirrhotic patients is 2, with sensitivities and specificities being 96.5% and 87%, respectively. An ALBI score below 2 would have spared 15.8% of patients from EGDs, missing 4.8% of VNT.
Conclusion: The ALBI and ALBI-PLT scores are reliable predictors of esophageal varices in patients with liver cirrhosis.
PP-02-188
Ascites associates with exacerbation after endoscopic treatment for esophageal varices
Akihiko Nishimura and Akio Moriya
Mitoyo Jeneral Hospital, Kannonji, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Esophageal varices, one of the complications of cirrhosis, sometimes treated endoscopically, often recur and require re-treatment. We investigated factors associated with exacerbations.
Materials and Methods: Forty-four patients treated endoscopically at our institution since 2014 were analyzed regarding the endpoint of exacerbation requiring re-treatment, rupture, or death, and the associated factors using a Cox proportional hazards model.
Results: The patients were 57% male, median age 69 years. There were 27% with alcoholic liver injury, 5% with hepatocellular carcinoma, 30% with HCV infection, 5% with HBV infection, 9% with autoimmune hepatitis, 18% with primary biliary cholangitis, and 16% with fatty liver as complications.
Exacerbation of varices and death were combined in 29 cases (66%), of which 16 were deaths.
In univariate analysis, ascites effusion and Child-Pugh score were significant factors, while multivariate analysis with these factors showed that a small or large amount of ascites effusion was a significant independent factor (hazard ratio, 2.394; 95% confidence interval, 1.0206 - 5.614).
Conclusion: Ascites was an exacerbating factor after endoscopic treatment for esophageal varices.
PP-02-189
Frequency of Minimal Hepatic Encephalopathy in Cirrhotic patients Attending a Tertiary Level hospital
MD Nuruzzaman1 and Majharul Haq2
1Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;2Department of Gastroenterology, Dhaka Medical College, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Minimal hepatic encephalopathy (MHE) is defined as patients with normal mental and neurological examinations but with several neuropsychiatric and neurophysiological defects identified by psychometric tests. Minimal hepatic encephalopathy (MHE) has implications for health-related quality of life as well as for survival of cirrhotic patients. This study intends to investigate the frequency of minimal hepatic encephalopathy in cirrhotic patients in a tertiary level hospital in Bangladesh.
Methods: This was an observational cross-sectional study done in BIRDEM General Hospital from August 2013 to July 2014. Eighty- five patients were selected by non-random sampling. Demographic, clinical and biochemical data were obtained. Cognitive functions were tested by using Bangla adaption of mini mental state examination (BAMSE) to ensure normal mental and neurological state AND psychometric tests- Number connection tests-A (NCT-A) and Digit Symbol Test (DST) were done for diagnosis of MHE.
Results: The total number of study patients was 85, among then 52 were male and 33were female. Mean age of the patients 55.78(+ 10.30) years. The frequency of MHE was 64.7%. MHE varied with different Child Turcot Pugh scores (CTP-B-35.2% and CTP-C -72.05%, P= 0.005). There was significant difference between MHE positive and negative groups in terms of mean platelet count, prothrombin time, serum ammonia, albumin and sodium level.
Conclusions: Cirrhotic patients were found to have a high frequency of MHE that is proportionate to the degree of liver function. Proper management should be implemented to avoid MHE among cirrhotic patients.
PP-02-190
APRI and FIB-4 Index in the Diagnosis of Chronic Liver Disease: An Analytical Cross-sectional Study
Federico Iv Peralta, Marc Jusenn Yumena, Sarah Jean Bellido and Juliet Cervantes
St. Luke's Medical Center -Quezon City, Metro Manila, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Chronic liver disease (CLD) involves progressive liver function deterioration, with late-stage liver cirrhosis characterized by fibrosis and architectural distortion, often necessitating transplantation. While early-stage reversibility exists, most advanced cases are irreversible. Liver elastography is a valuable noninvasive diagnostic tool, but its limited availability in resource-constrained areas necessitates alternative fibrosis scoring systems. This study aims to assess the accuracy of the Aspartate Aminotransferase to Platelet Ratio Index (APRI) and Fibrosis-4 Index (FIB-4) in diagnosing CLD among adults with suspected or confirmed CLD.
Materials and Methods: This observational, analytical cross-sectional study included adult patients (≥18 years) with suspected or confirmed CLD who underwent liver elastography at St. Luke’s Medical Center, Quezon City, from January 2015 to November 2022. The study analyzed 208 samples to compare APRI and FIB-4 scores against liver elastography results.
Results: The Receiver Operating Characteristic (ROC) curve revealed that FIB-4 (AUC 0.76) had a superior discriminatory ability for predicting F4 fibrosis compared to APRI. The optimal APRI cut-off for predicting F4 fibrosis was >0.5835 (accuracy 71.64%, sensitivity 77.78%, specificity 66.06%), while the optimal FIB-4 cut-off was >3.117 (accuracy 75.48%, sensitivity 67.78%, specificity 82.57%). Both APRI and FIB-4 scores were positively correlated with liver elastography METAVIR stages.
Conclusion: Although APRI and FIB-4 have limitations in accurately predicting advanced fibrosis, their positive correlation with liver elastography METAVIR stages makes them useful tools for identifying individuals at risk of advanced liver fibrosis or cirrhosis, aiding in the prioritization of further evaluation and intervention.
PP-02-191
Comparison of LMR with CPS and MELD Score Determining Severity and Outcome in Cirrhotic Patient
Mohammad Mahatabur Rahman
Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Abstract
Objectives: To evaluate the neutrophil-to-lymphocyte ratio (NLR) and lymphocyte-to-monocyte ratio (LMR) for the prediction of prognosis of liver cirrhosis patients.
Material and methods: This Cross-sectional analytical study was conducted in Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from July 2020 to December 2020. Total 40 compensated cases and 40 decompensated cases were included and information regarding clinical profile, laboratory parameters was collected. LMR, NLR, MELD score and CP score were calculated both groups. Correlation between LMR, NLR and CP/MELD score were established and cut-off values of LMR and NLR were obtained.
Results: The mean value of NLR was significantly higher in decompensated cirrhosis patients than that of compensated ones and LMR was significantly higher in compensated cirrhosis patients(p<0.001). NLR had a significant strong positive correlation with CPS scores (r=0.774, p<0.05) whereas LMR had a significant negative correlation with CPS scores (r = -0.732, p<0.05). At a cut-off value ≥5.61, NLR had 80% sensitivity and specificity & LMR had 64% sensitivity & 79% specificity, cut-off value was found ≤ 2.45. A cut-off value of MELD score ≥ 26.5 was 100% sensitive but 80% specific in the prediction of death among patients with cirrhosis. Those parameters were capable of presaging the early prognosis of liver cirrhosis patients. Of them, NLR was found most precise.
PP-02-192
A study of the profile of infections in the hospitalized patients with cirrhosis of liver.
Rhimanshu Soni, Preetam Nath and A.C Anand
Department of Gastroenterology & Hepatology, Kalinga Institute Of Medical Sciences, Kushabhadra Campus, KIIT University, 751024, Bhubaneswar, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Bacterial infections are common in patients with liver cirrhosis. Infections worsen the outcomes in terms of organ failures, length of hospital stay & mortality. We here aimed to study the profile and impact of bacterial infections on outcomes in terms of 28 days mortality, length of hospital stay & organ failure in patients with liver cirrhosis.
Methods: The study subjects were inpatients with cirrhosis of liver in KIMS Bhubaneswar from September 2022 to February 2024. All the demographic, clinical, microbiological & treatment data were collected at the time of admission. Patient were followed up until death or for 28 days from the day of admission.
Results: A total of 200 patients with cirrhosis of liver were enrolled in this study out of which 128 had infection (64%). Most frequent infection were UTI (46.1%) followed by SBP(25%), spontaneous bacteremia (14.1%) & others 14.8%. Microbiological sampling was performed and culture-positive results were obtained in (n=115/200, 57.5%). Among them E.coli was the most frequent organism isolated (n=47/115,40.86%). Multi drug-resistant (MDR)-bacteria were found in (n=59/115, 51.3%). 28 days mortality (33.6% vs 13.8% p=0.002), length of hospital stay (9.57vs7.99days, p=0.046) were significantly higher in patients with infections than those without. Among all organ failures, renal, circulatory & respiratory failure were significant more in patient with infection(p<0.05).
Conclusion: The present study showed high prevalence of bacterial & MDR infection in patient with cirrhosis of liver. Patients with infections had higher 28 days mortality, length of hospital stay & organ failures.
PP-02-193
Long-term outcomes of L-carnitine Administration in Hyperammonemia Patients with Liver Cirrhosis
Faculty Of Medicine, Kagawa University, Miki-cho, Kagawa, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: There are few data on the long-term efficacy of L-carnitine administration in improving blood ammonia concentration (BAC) and preventing recurrence of hepatic encephalopathy (HE). This study aimed to determine the effects of long-term L-carnitine administration on BAC and HE.
Methods: Of 444 patients with L-carnitine administration from April 2012 to March 2021, we enrolled 242 patients with hyperammonemia or HE. A multicenter retrospective study was conducted to determine the long-term efficacy of L-carnitine administration.
Results: Median BAC at the start and at 12, 24, 48, 96,144, and 192 weeks was 123, 95.5, 88, 83, 96, 82, and 86 μg/dL, respectively. BAC were significantly lower than those at the start (p<0.05, respectively). The median improvement time to normalization of BAC was 100 days. The 113 patients with a history of hospitalization for HE followable for 2 years before and after L-carnitine therapy had a total of 183 hospitalizations for HE before treatment, compared with 62 hospitalizations after treatment (p<0.001). The event incidence rate due to HE, such as emergency hospitalization, emergency transport, or additional medications, at 60, 180, 360, 720, and 1080 days after L-carnitine administration was 5.7%, 16.9%, 20.3%, 29.5% and 38.5%, respectively. The change in MELD scores before and after L-carnitine administration was statistically significant, while other variables (liver reserve function, nutritional status, and muscle index) did not show significant changes. Finally, the median survival time was 880 days.
Conclusions: Long-term L-carnitine administration is effective in hyperammonemic patients, and can reduce hospitalizations and events due to HE.
PP-02-194
Gut Akkermansia muciniphila mitigates hepatic fibrosis by modulating riboflavin metabolism through the gut-liver axis
Lu Zhang and Cui-Hua Lu
Department of Gastroenterology, Afliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Akkermansia muciniphila (AKK), a gut bacterium, is gaining recognition for its potential in treating metabolic disorders, including its ability to combat liver fibrosis.
Materials and Methods: In this study, we employed a CCl4-induced hepatic fibrosis mice to explore the mechanisms of AKK in intervening hepatic fibrosis. Mice treated with AKK showed effective reversal of hepatic fibrosis, including collagen deposition, inflammation, and hepatic injury, which was attenuated in hepatic fibrosis mice treated with antibiotics.
Results: The therapy enhanced oxidative stress and vitamin metabolism along the gut-liver axis. This led to the deactivation of hepatic stellate cells and a modification in gut microbiota composition, alongside an increase in riboflavin transport from the gut to the liver. These metabolic improvements occurred with an increase in riboflavin levels transported from the gut to the liver. Riboflavin displayed similar beneficial metabolic effects in vitro and in mice, effectively ameliorating hepatic fibrosis. Riboflavin itself showed promising effects, mirroring AKK's benefits by alleviating hepatic fibrosis.
PP-02-195
Safety And Efficacy Of Lenvatinib And Sorafinib In Patients With Advanced Hepatocellular Carcinoma.
Nazish Butt1 and Gulzar Khan2
1Jinnah Postgraduate Medical Centre, Karachi, Pakistan;2JPMC, Karachi, Pakistan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Sorafenib has been the standard targeted therapy for advanced HCC, but recent studies suggest Lenvatinib might be a more promising option. This study aimed to compare the safety and efficacy of Lenvatinib and Sorafenib in treating patients with advanced HCC.
Materials and Methods: This was a prospective study our institution between August 2023 to April 2024. Patients were divided into two groups based on the targeted therapy received: Sorafenib or Lenvatinib. The primary outcome was overall survival. Adverse events (AEs) were assessed within 6 weeks of treatment initiation using common terminology criteria for AEs version 4.0.
Results: A total of 36 patients were included in the study. Twenty patients received Sorafenib and sixteen received Lenvatinib. The two groups showed no significant differences in baseline characteristics after propensity score matching. Lenvatinib was associated with a longer overall survival time three months more then that of sorafenib group(p-value <0.0032).The most common AEs in the sorafenib group were loss of appetite (66%),fever (50.0%), diarrhea (45%) and hand-foot skin reaction (30%). In contrast, the most common AEs in the lenvatinib group were loss of appetite (55%), hypertension (24%), hand-foot skin reaction (18%), and proteinuria (20%).
Conclusions: This study suggests that Lenvatinib may be a more effective treatment option compared to Sorafenib for patients with advanced HCC. Lenvatinib treatment resulted in significantly better objective response rates and overall survival. Sorafenib and Lenvatinib exhibit distinct AE profiles in patients with advanced HCC.
PP-02-196
Extrahepatic recurrence after surgical resection of hepatocellular carcinoma without intrahepatic hepatocellular carcinoma: Multi-institutional observational study
Sanghyuk Jung
Chonnam National University Hospital, Gwangju, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Extrahepatic recurrence (EHR) is a known poor prognostic factor for hepatocellular carcinoma (HCC). While EHR can occur in high-risk patients post-surgery, its characteristics without concurrent intrahepatic HCC are less understood. This study examines the clinical features and risk factors for EHR without remnant intrahepatic HCC at diagnosis.
Materials and Methods: From January 2004 to December 2019, 1,069 treatment-naive patients who underwent curative hepatectomy for HCC at four tertiary academic hospitals were assessed. After excluding those with intrahepatic recurrence (IHR) or combined EHR and IHR, and those with insufficient clinical records, 569 patients were enrolled. The median follow-up duration was 3.91 years. Multivariate analysis via Cox regression identified variables associated with EHR.
Results: Thirty-eight patients developed EHR without remnant intrahepatic HCC during a median follow-up of 1.04 years. EHR patients experienced significantly earlier initial HCC recurrence than those without EHR (1.73 vs. 4.43 years). Multivariate analysis revealed that EHR patients had higher venous and/or lymphatic involvement (HR 2.418, p=0.020), tumor necrosis (HR 2.592, p=0.009), and higher initial tumor stages beyond the Milan criteria (HR 3.008, p=0.001). EHR was strongly associated with poor survival (HR 14.044, p<0.001). The cumulative rates of EHR and survival correlated with the number of risk factors.
Conclusion: EHR without remnant viable HCC can occur in many patients post-surgical resection. Close monitoring for EHR is essential in this high-risk group, even without evidence of intrahepatic HCC.
PP-02-197
The Clinical Features and Mortality of Hepatocellular Carcinoma associated Hepatitis B Patients in Tertiary Hospital
Ulfa Kholili, Kartika Wensdi Renantriandani, Vembi Rizky Rahmawati, Febrian Ramadhan Pradana, M Alwi Alaydrus, Awwaliyah Azmi Izzati, M Zulfikar Defianto, Poernomo Boedi Setiawan and Muhammad Miftahussurur
FK Unair-RSUD Dr. Soetomo, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives and Aim: Hepatocellular carcinoma (HCC) is considered a significant burden, and its associated rate of mortality is increasing. The aim of this study to observe clinical features and mortality of HCC associated Hepatitis B.
Material and methods: An observational study with cohort prospective, total sampling method, follow up January 2023 to July 2024, descriptive data analyzing.
Result: This study involved 129 HCC related Hepatitis B patients consist of 82.9% male, 76% Javanese ethnic and average age 51.86 y.o. The most frequent clinical manifestation were abdominal pain (82.2%) and hepatomegaly (82.9%), comorbid hypertension (20.9%), CTP score A (45.7%), and presented BCLC staging consecutively BCLC-A (4.7%), BCLC-B (41.1%), BCLC-C (34.1%), and BCLC-D (20.2%). The tumor characteristics were single nodule (52.7%), right lobe (54.3%), presence of portal vein thrombus (37.2%), tumor size 11.9 cm. Laboratory findings were Hb 12.13, Albumin 3.34 mg/dL, ALBI Score -1.89 (grade 2), and the median of thrombocyte 260.000, Bilirubin 1.30 mg/dL, SGOT 123u/L, SGPT 49u/L, AFP 3,450 ng/ml, and HBV DNA 1.8 x 10^4 IU/mL. During 18 months observation, 29 (22.4%) patients were found died, with survival rate was 4.8 months, which duration of mortality less than 6 months (79.3%), during 6-12 months (17.2%), more than 12 months (3.4%) were observed. The most cause of mortality was liver failure.
Conclusion: The mortality of HCC related Hepatitis B was high and the survival rate was 4.8 months. This result similar with other studies of HCC with any different etiologies.
PP-02-198
The Efficacy and Safety of Atezolizumab and Bevacizumab Combination Therapy for Advanced Hepatocellular Carcinoma
Ji Hoon Kim and Yang Jae Yoo and Eunho Choi and Kwan Soo Byun
Korea University Guro Hospital, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background & Aims: Atezolizumab-bevacizumab combination therapy has been approved for first-line systemic chemotherapy in advanced hepatocellular carcinoma. Real-world retrospective analysis was performed to evaluate the effectiveness and safety of the chemotherapy.
Methods: A single-center, retrospective cohort study was performed. Between January 2020 and July 2023, patients with advanced hepatocellular carcinoma who received atezolizumab-bevacizumab systemic therapy were enrolled. Response to the therapy was assessed with mRECIST criteria. Evaluation of first radiologic response, best overall response, progression-free survival, and overall survival was done. Safety data were collected. Also, prognostic factors for first radiologic response, progression-free survival, and overall survival were also assessed.
Results: A total of 70 patients were investigated. Baseline characteristics showed a median age of 63 years, male dominance (89%), and viral etiology (84%). Four patients achieved complete response and 19 reached partial response when evaluated by best overall response. Response duration was 4.7 months (range 1.5-25.5). Median progression-free survival was 4.1 months (95% CI, 3.7-6.7), and median overall survival was 24.6 months (95% CI, 11.6-). Age and tumor extent of over 50% of the liver was predictive of progression-free survival (p=0.009 and p=0.007, respectively) Tumor extent of over 50% of the liver and ALBI score were predictive of overall survival (p=0.025 and p=0.004, respectively).
Conclusion: Atezolizumab-bevacizumab therapy showed valuable outcomes and tolerable safety profiles in real-world advanced HCC patients. The outcome and safety results were comparable to previous studies.
PP-02-199
The Role of Glucose Transporters in Tumor Recurrence and Progression in HCC Patients Undergoing TACE
Hui-pu Liu
Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: We investigates the role of glucose transporters (GLUTs) in hepatocellular carcinoma (HCC) progression, particularly in relation to transarterial chemoembolization (TACE) treatment. We explore the metabolic phenomenon of aerobic glycolysis in HCC, mechanisms of GLUTs-related tumorigenesis, and the influence of hypoxia on tumor behavior.
Methods: Using the GSE104580 dataset from the Gene Expression Omnibus, we compared gene profiles between TACE responders and non-responders, identifying significant differences in GLUT1, Gal-3, and CD133. We enrolled HCC patients undergoing surgery or TACE, with 16 surgery cases and 4 TACE cases. Tumor specimens were analyzed for markers such as HIF-1α, GLUT1, Gal-3, and CD-133. In cell line experiments, Hep3B and SNU-387 were studied under hypoxic (1% O2) and normoxic (21% O2) conditions, and the GLUT1 inhibitor (BAY-876) was tested.
Results: Significant differences in GLUT1, Gal-3, and CD133 expression were observed between TACE responders and non-responders. Tumor tissues had higher levels of HIF-1α, GLUT1, Gal-3, and CD-133 compared to non-tumor tissues. Under hypoxia, Hep3B and SNU-387 exhibited increased GLUT1 and HIF-1α expression. Hep3B's cell viability increased under hypoxia, while SNU-387's viability exceeded 200% by 48 hours. IC50 values for BAY-876 varied under normoxia and hypoxia.
Conclusion: TACE-induced hypoxia increases GLUT1 and cancer stemness markers, potentially accelerating tumor growth and contributing to poor outcomes. GLUT-1 inhibitors may suppress liver cancer cell growth in hypoxic environments, offering a potential solution for TACE resistance. This research highlights the importance of GLUTs in HCC progression and their potential as therapeutic targets, aiming to improve treatment strategies and patient outcomes.
PP-02-200
Evaluating the Prognostic Utility of the Geriatric Nutritional Risk Index in Intermediate-Stage Hepatocellular Carcinoma Patients
Imelda Maria Loho1, Kevin Tjoa2, Lianda Siregar1, Agus Waspodo1, Irsan Hasan2 and Rino Alvani Gani2
1Dharmais National Cancer Center, Indonesia;2Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The geriatric nutritional risk index (GNRI), which is based on body weight, height, and albumin level, serves as a prognostic indicator in various diseases beyond elderly patients. However, its role in hepatocellular carcinoma (HCC) patients remains unclear.
Materials and Methods: A retrospective cohort study was conducted at two tertiary hospitals in Jakarta, Indonesia. Patients diagnosed with intermediate-stage HCC that allowed GNRI calculation from January 2015–December 2022 were included. Analysis on one-year overall survival (OS) was conducted using the Kaplan-Meier method, and p-values were derived from the log-rank test. Stratification was based on sex, treatment, cirrhosis, Child-Pugh class, alpha-fetoprotein (AFP), and up-to-7 status.
Results: Of the 125 patients, 61.6% were considered low-risk (GNRI ≥ 92). The one-year overall survival (OS) was 72.8% (low-risk: 77.9% vs. high-risk: 64.6%), with a mean OS of 293 days (95% CI: 273–315). The low GNRI group had a mean OS of 308 days (95% CI: 284–333), with the median OS not reached. Conversely, those with a high GNRI had a mean OS of 268 days (95% CI: 229–307) and a median OS of 331 days. There were no significant survival differences within the overall cohort (p = 0.068), nor within any strata, except for patients with AFP >200 ng/mL (mean OS 329 vs. 236 days; p = 0.002).
Conclusion: There are no differences in survival between low and high-risk nutritional status in HCC patients based on GNRI. Using GNRI for prognostication may be suitable for specific populations, such as patients with AFP > 200 ng/mL.
PP-02-201
Artificial Intelligence in the Prediction of Trans-arterial Chemoembolization Outcomes pre-procedure: An Updated Systematic Review
Kai Yi Benjamin Nah1,2, Elina Cho2, Michelle Law2, Jia Hao Law4, Alfred Wei Chieh Kow2,3,4, Cheng Han Ng1, Mark Muthiah1,2,3 and Daniel Qingyao Huang1,2,3
1Division of Gastroenterology and Hepatology, Department of Medicine, National University Hospital, Singapore;2Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore;3National University Centre for Organ Transplantation, National University Health System, Singapore;4Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, National University Hospital, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Intermediate stage HCC is a heterogenous group with varying tumor characteristics and responses to trans-arterial chemoembolization (TACE). The BCLC staging system currently uses radiologist-reported tumor characteristics to determine TACE suitability such as tumor size and number, presence of portal blood flow. However, there is a need for more precise methods to predict TACE response to improve patient selection and post-TACE management. This systematic review assesses the performance of AI-based models, including radiomics, in predicting TACE outcomes in HCC patients and evaluates their potential clinical application.
Materials and Methods: A comprehensive search was conducted in Medline and Embase databases for articles on AI predicting HCC response to TACE from inception to April 7, 2024. Studies were analyzed for their predictive model's effectiveness.
Results: The review included 64 articles with 13,412 TACE-treated patients. These studies used various imaging modalities, primarily CT and MRI, to develop predictive models with machine learning (ML) and deep learning (DL) algorithms. Results indicate that AI-based models, particularly those using CT imaging, show high predictive value for TACE outcomes, with some models achieving area under the curve (AUC) values above 0.90. Models incorporating radiomics—automatically extracted features from medical images—outperformed those relying on manual measurements by radiologists. Some models also integrated clinical and laboratory data, enhancing predictive accuracy.
Conclusion: AI-based models show potential in improving TACE outcome predictions, aiding in patient selection and management. However, further validation in diverse clinical settings is needed to confirm these results and facilitate AI integration into routine clinical practice.
PP-02-202
Initial Presentation of Hepatocellular Carcinoma as a Hepatic Cyst with Peritoneal Metastases
Tatag Primiawan
Gadjah Mada University, Magelang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Cystic hepatic lesions are common and range from benign to potentially lethal conditions. Distinguishing between malignant and benign cystic lesions is challenging, even with advanced imaging. Cystic degeneration is a rare manifestation of hepatocellular carcinoma (HCC), making diagnosis difficult without microscopic biopsy examination.
Case Illustration: A 57-year-old man presented to the Emergency Room with hematemesis, abdominal pain, vomiting, increasing abdominal distension, and weight loss over 4 months. He had no history of hepatitis, hepatotoxin exposure, or alcohol abuse but had a history of repeated transfusions. Physical examination revealed abdominal tenderness, meteorism, and ascites. Tests for chronic liver disease were negative, and ultrasonography showed a non-cirrhotic liver. Barium follow-through revealed a gastric ulcer. MSCT Abdomen showed hepatomegaly with a hepatic cyst and multiple lymphadenopathy. Esophagogastroduodenoscopy revealed a giant gastric ulcer with mucosal dysplasia, and biopsy showed mild chronic gastritis. During laparotomy, biopsies from the omentum, appendix, peritoneal wall, liver, and mesentery indicated high-grade carcinoma metastasis. Immunohistochemistry confirmed primary hepatocellular carcinoma of the liver with metastasis to the omentum, peritoneal wall, mesentery, and appendix.
Conclusion: Hepatocellular carcinoma (HCC) can rarely present as cystic lesions without liver cirrhosis. Proposed mechanisms for these cystic changes include arterial thrombosis, inflammation, and rapid tumor growth, though the exact cause remains unclear. Some hypothesize immune-related mechanisms involving interleukin-18 inducing interferon-γ release from T cells and natural killer cells, leading to tumor necrosis. Pathological evidence shows an inflammatory response with rapid leukocyte infiltration and massive lymphoid infiltration.
Demographic and Clinicopathologic Factors of Patients with Hepatocellular Carcinoma at Tertiary Hospital in Indonesia
Febry Rahmayani and Suyata Suyata
Mohammad Hoesin General Hospital Palembang/Faculty of Medicine Sriwijaya University, Palembang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: The purpose of this study is to characterize the current state of hepatocellular carcinoma (HCC) at Mohammad Hoesin General Hospital Palembang, Indonesia.
Materials and Methods: This is a retrospective study involving 98 patients, done at Mohammad Hoesin Hospital Palembang from January to August 2023. Laboratory and imaging examinations were done on each patient. This study was performed to review the demographic characteristic, clinicopathologic data, treatment strategies, and outcomes of HCC patients.
Results: Ninety-eight HCC patients consist of 72 males (77.4%) and 21 females (22.6%) with an average age of 53.47±11.28 years, from which 18 participants were underweight (18.2%), 17 participants were overweight (18.3%) and 13 participant were obese (32.3%). The majority of causes of HCC are hepatitis B virus (62.4%), hepatitis C virus (5.4%), and other causes (32.3%). Based on the ALBI ratio, Grade 1: 69.9%, Grade 2: 28.0%, and Grade 3: 2.2%. A total of 75.3% participant present with large tumor (≥ 5 cm), 62.4% participant present with multiple nodules, and about 44.1% patients had tumor metastasis or thrombus. Most of the patient with advanced disease got best supportive care (80.6%). Sixteen (17.2%) patients died of their disease, 77 (82.8) patients are alive 3 months after diagnosed.
Conclusion: Most patients with HCC at Mohammad Hoesin Palembang with advanced disease; however, multiple local and systemic treatments were offered.
Keywords: Hepatocellular carcinoma
PP-02-204
Comparing survival outcomes in less than 2cm and 2-3cm lesions of hepatocellular carcinoma (retrospective study)
Vujwal Roy, Anshid Nazar, Jeby Jacob, Jeffey George and KH Ismail Siyad
Aster Medcity, Kochi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: There's limited data from India on the difference between less than 2cm and 2-3cm lesions in Hepatocellular Carcinoma(HCC)
Objectives: To compare Overall Survival, disease characteristics in less than 2 cm and 2-3cm lesions of HCC
Materials and Methods: Data of 259 patients were analyzed from 2016 to 2024. 77 patients had less than 3cm diameter. Diagnosis was made based on reports of Triple phase CT(or)MRI of Liver, AFP, or Biopsy. Patients who had undergone transplantation were excluded, and those who had been under follow up for atleast 4 years or died were included.
Results: Of the 77 patients, 29 patients had less than 2cm lesions, while 48 patients had 2-3cm lesions. The median(IQR) of AFP, INR, Bilirubin were 6.7(3.8,24.9); 1.31(1.18,1.6); 1.3(0.83,2.55) and 10.34(3.79, 1.36(1.22,1.68), 1.2(0.87,2.18) in less than 2cm lesions and 2-3cm lesions respectively.
Mean±SD of Albumin was 3.31±0.72 and 3.42±0.59 in less than 2cm, and 2-3cm lesions respectively. Mean±SD of Overall Survival in less than 2cm and 2-3cm lesions was 35.25±30.65 months and 29.14±25.08 months respectively.
AFP, INR, Bilirubin and albumin were not statistically significant in the two groups(p 0.563, 0.433, 0.538, 0.476). Overall survival was non significant in the two groups (0.444).
Conclusion: While previous studies suggest that survival outcome was better in less than 2cm when compared to 2-3cm lesions.
The difference in overall survival, AFP, Bilirubin, INR and Albumin was statistically insignificant in this study.
To conclude, less than 3cm lesions irrespective of whether 2-3cm or less than 2cm have similar overall survival.
PP-02-205
Comparison of Alpha-Fetoprotein Levels in Viral and Non-Viral Related Hepatocellular Carcinoma
Mutmainna Said1, Muhammad Luthfi Parewangi2,3, Susanto H. Kusuma2,3, Fardah Akil2,3, Nu’man AS Daud2,3, Rini Rachmawarni Bachtiar2,3 and Amelia Rifai2,3
1Internal Medicine Specialist Program, Departement of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;2Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;3Centre of Gastroenterology-Hepatology HAM Akil Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer worldwide and a major cause of mortality. It is commonly associated with chronic liver diseases, particularly HBV infection, HCV infection, and other etiologies. Approximately 78,200 new cases are diagnosed each year, and the incidence of HCC will increase, estimated peak in the year 2030. Alpha-fetoprotein (AFP) is one of biomarker used for HCC screening and monitoring, although its levels can vary depending on the etiology and severity of the disease. The aim of this study is comparing AFP levels based on the degree of HCC of viral and non-viral etiology
Materials and Methods: A cross-sectional study was conducted of HCC patients from January to June 2024 at Dr. Wahidin Sudirohusodo Hospital. Patients were categorized based on etiology (viral/non-viral) and The Barcelona Clinic Liver Cancer staging. AFP levels and BCLC stage were statistically analysed using the Kruskal-Wallis test.
Results: The study included 104 HCC patients, with 86 having viral etiology and 18 non-viral etiology. Patients with viral HCC were more likely to be male, older (≥ 55 years), have larger tumors (>10 cm), more frequent portal vein thrombosis, higher BCLC stages, and elevated AFP levels (>200 ng/ml). AFP levels were significantly higher in the viral group compared to the non-viral group. Additionally, AFP levels increased significantly with the progression of BCLC stages with p-value 0.003.
Conclusion: Based on the degree of HCC, AFP levels significantly difference in viral related compare to non-viral related cases.
PP-02-206
tRF-3a-Pro facilitated the progression of hepatocellular carcinoma by promoting MYC mRNA stability through hnRNP K
1Zhongshan Hospital, Fudan University, Shanghai, China;2Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Xiamen, China;3Shanghai Geriatric Medical Center, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: In this study we aimed to explore the molecular mechanism of tRF-3a-Pro regulating the biological function of HCC cells through RNA binding proteins.
Methods: The effect of tRF-3a-Pro on biological function of HCC cells was observed by CCK-8, EdU, clone formation and cell cycle assays. RNA pull-down and protein mass spectrometry assay were used to screen for proteins that bind to tRF-3a-Pro. FISH and immunofluorescence experiments were used to determine the intracellular localization of tRF-3a-Pro and hnRNP K. We obtained downstream genes through bioinformatics analysis. The half-life of mRNA of downstream gene MYC was evaluated through ACTD treatment. RIP experiments were used to evaluate the effect of tRF-3a-Pro on the binding ability of hnRNP K to MYC mRNA.
Results: tRF-3a Pro could promote the proliferation of HCC cells. We screened hnRNP K as a tRF-3a-Pro binding protein and validated the interaction between tRF-3a-Pro and hnRNP K within the nucleus. Knocking down hnRNP K could inhibit cell proliferation and coloy formation ability as well as tRF-3a-Pro. MYC was identified as downstream gene co-regulated by tRF-3a-Pro and hnRNP K. Subsequent studies revealed that tRF-3a-Pro and hnRNP K decreased the expression of MYC by regulating the stability of MYC mRNA. Finally, RIP experiments results suggested that tRF-3a-Pro reduced the half-life of MYC mRNA by affecting the binding ability of hnRNP K to MYC mRNA.
Conclusion: tRF-3a-Pro enhances the stability of MYC mRNA by promoting its combination to hnRNP K, thereby regulating the proliferation of HCC cells.
PP-02-207
Expression and diagnostic value of tRNA derived fragment tRF-3a-Pro in HCC
1Zhongshan Hospital, Fudan University, Shanghai, China;2Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Xiamen, China;3Shanghai Geriatric Medical Center, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This study aims to identify the expression characteristics of tsRNAs in HCC tissue, screen and verify the upregulated tsRNAs in tumor tissue and serum of HCC patients, and preliminarily evaluate the diagnostic value of serum tsRNA for HCC.
Methods: We performed tsRNA sequencing in 5 pairs of HCC tumor and adjacent tissues, and screened for differentially expressed tsRNAs by p-value and fold changes. The top 4 upregulated tsRNAs in the sequencing results were validated in HCC tumor/adjacent tissues and serum of HCC patients/control population through quantitative real-time polymerase chain reaction (qRT-PCR). A diagnostic model of alpha fetoprotein (AFP) and tRF-3a-Pro was established for HCC by logistic regression, with liver cirrhosis patients and healthy control individuals as control groups.
Results: The main differential expressioned tsRNA in HCC tissues were tRF-3a and tiRNA-5, with the main upregulated subtype being tRF-3a. The upregulation of tRF-3a-Ala-AGC, tRF-3a-Tyr-GTA, tRF-3a-Pro-CGG, and tRF-3aAla-CGC in 28 pairs of tumor/adjacent tissues was confirmed by qRT-PCR. Only the amount of serum tRF-3a-Pro in HCC patients was significantly increased compared with the control group (AUC = 0.8393, cutoff value = 1.4197). The combination of AFP with tRF-3a-Pro improved the AUC to 0.8884 in the training population with combining predictors = -1.5897 + 0.433 × tRF-3a-Pro + 0.0821 × AFP (cutoff value = -0.2441). And validation was conducted in the validation set population (AUC = 0.8805).
Conclusion: tRF-3a-Pro is upregulated in tumor tissue and serum of HCC patients, and performs well in the diagnosis of HCC, especially when combined with AFP.
PP-02-208
HCC picked up through screening showed better survival outcomes
Benedix Sim
National University Hospital, Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
We conducted a multicentre screening cohort study evaluating survival outcomes between screening and non-screening populations.
Methods: In this multicentre retrospective cohort study, HCC patients diagnosed through ultrasound screening, incidentally, or symptomatically were enrolled from 6 tertiary healthcare institutions. Survival outcomes were evaluated between screening and non-screening populations, with secondary outcomes stratifying the populations based on HCC aetiology, cirrhosis status, age, sex, ethnicity, and BCLC staging.
Results: In total, 1185 patients were included in the analysis (178 MASH HCC, 168 alcohol-associated HCC, 422 HBV HCC, 395 HCV HCC, and 128 patients with unknown etiology), 975 patients were diagnosed through screening while 210 patients were diagnosed incidentally or symptomatically. Survival analysis showed statistically significant differences in restricted mean survival time (RMST) based on screening status (p < 0.001), with an RMST of 8.369 years (95% CI: 7.886 - 8.851) amongst screening patients and 4.401 years (95% CI: 3.479 - 5.323) amongst non-screening patients. By aetiology, HBV HCC had the largest difference between screening and non-screening cohorts (RMST ratio: 1.966, 95% CI: 1.496 - 2.583, p < 0.001), followed by ALD HCC (RMST ratio: 1.765, 95% CI: 1.262 - 2.469, p = 0.001) and HCV HCC (RMST ratio: 1.687, 95% CI: 1.200 - 2.374, p = 0.003). Multivariate analysis showed significant differences between screening and non-screening populations after adjusting for cirrhosis, gender, age, and ethnicity.
In conclusion, HCC surveillance is associated with improved early detection and survival in patients.
PP-02-209
Clinical outcomes and prognostic factors of transarterial chemoembolization in patients with solitary large hepatocellular carcinoma
Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: There is a scarcity of data regarding the outcomes of Transarterial chemoembolization (TACE) in treatment-naïve hepatocellular carcinoma (HCC) patients with tumors larger than 10 cm. This study aimed to evaluate the efficacy of TACE and identify clinical factors that predict outcomes in this patient subgroup.
Materials and Methods: We retrospectively analyzed treatment-naïve patients with a solitary large HCC (defined by a tumor size of at least 10 cm) and no extrahepatic metastasis. All patients received TACE as their initial treatment. Overall survival (OS) was calculated, and data on demographics, clinical specifics, tumor characteristics, and laboratory results were collected.
Results: 120 patients were included. The median age was 61.5 years (interquartile range (IQR): 7.8 years), with 104 patients (86.7%) being male. The median tumor diameter was 14 cm (IQR: 2.2 cm), and vascular invasion was present in 57 patients (47.5%). Median OS was 45 months (95% CI: 27 – 79 months), with OS rates of 69.2% at 6 months, 46.7% at 1 year, and 27.5% at 2 years. A larger tumor diameter was associated with increased mortality risk (OR 1.29, p = 0.04). Tumor rupture, portal vein invasion, underlying cirrhosis, and alpha-fetoprotein levels at diagnosis did not predict worse outcomes. Only 14 patients (11.7%) achieved complete radiologic tumor response with TACE as a monotherapy.
Conclusion: Treatment-naïve patients with a solitary large HCC larger, who underwent TACE as the initial therapy, showed reasonable overall survival rates. Larger tumor size was the only significant predictor associated with reduced survival outcomes.
PP-02-210
Therapeutic Effectiveness of Early Additional cTACE after DEB-TACE for Hepatocellular Carcinoma
Kagawa university, Ikenobe, Miki-town, Kita-gun, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Purpose: We have previously reported that early additional cTACE after drug-eluting beads TACE (DEB-TACE) significantly increased the CR rate compared to patients treated with DEB-TACE alone. In the present study, we investigated how early additional cTACE should be performed after DEB-TACE in patients with hepatocellular carcinoma (HCC) at a multicenter setting.
Methods: Twenty-seven patients with unresectable hepatocellular carcinoma (HCC) with a maximum tumor diameter of 30 mm or greater, Child-Pugh classification A-B, and ECOG-PS 0-2, who were treated at four institutions including our hospital between July 2014 and November 2022, were included in this study. The subjects undergone DEB-TACE first and cTACE as the second treatment. The overall response rate (ORR) and disease control rate (DCR) were evaluated retrospectively by mRECIST.
Results: The overall evaluation by initial DEB-TACE was CR/PR/SD/PD=0/27/0/0, and the overall response rate and disease control rate were 100%. The overall response rate was 75.0% and disease control rate was 91.7% in the group that received the next cTACE treatment earlier than DEB-TACE (CR/PR/SD/PD=1/8/2/1), whereas the overall response rate and disease control rate in the group that received the next cTACE treatment more than 3 months after conventional DEB-TACE were The overall response rate, CR/PR/SD/PD=2/3/6/4, overall response rate, and disease control rate were 33.3% and 73.3%, respectively, indicating a significant difference in overall response rate between the two groups (p=0.031).
Conclusion: In unresectable hepatocellular carcinoma, early additional cTACE after DEB-TACE can bring better therapeutic effect.
PP-02-212
The Impact of Metabolic Abnormalities on Prognosis of Lean Patients with Hepatocellular Carcinoma Receiving Surgery
Chih Hsuan Wang1,2,4, Kung Hung Lin3 and Jee Fu Huang4,5,6,7
1Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan;2Division of Gastroenterology and Hepatology, Tri-Service General Hospital, Taipei, Taiwan;3Division of Gastroenterology and Hepatology, Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;4Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;5Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;6Hepatitis Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;7Center of Excellence for Metabolic Associated Fatty Liver Disease, National Sun Yat-sen University, Kaohsiung, Taiwan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Obesity is associated with an increased risk of HCC. However, whether a higher body mass index (BMI) confers a survival benefit in HCC patients remains unclear. We conducted this study to investigate survival in HCC patients after hepatic resection according to BMI and analyzed the impact of metabolic abnormalities between lean and non-lean HCC patients.
Materials and methods: This retrospective cohort study recruited pathologically-proven HCC patients receiving surgical resection between 2013-2021 in a medical center in southern Taiwan. We categorized the patients into four groups: BMI ≥23(also called non-lean) with ≥ 1 cardiometabolic risk factor (CMRF) group (A), BMI ≥23 without CMRF group (B), normal BMI(also called lean) with ≥ 1 CMRF group (C), and normal BMI without CMRF group (D). We also analyzed the etiologies, fibrosis stage, liver-related events, cardiovascular events and 5-year survival rate of the HCC.
Results: A total of 1,208 HCC patients were recruited. Patients of group C had a significantly higher proportion of HCV infection (38.1%). Group C had a significantly lower 5-year survival rate than patients of group A (p = 0.007) and group B (p < 0.001). In the subgroup analysis, group C had the lowest 5-year survival rate in both the HCV subgroup and the non-B-non-C etiologies.
Conclusion: The lean individuals with metabolic abnormalities have a lower five-year survival rate. This phenomenon is more pronounced in patients with HCV or non-B-non-C etiologies. Therefore, rigorous follow-up with interventions for metabolic abnormalities are recommended for lean patients with HCC.
PP-02-213
DCAF13 promotes the progression of hepatocellular carcinoma through the AKT/PI3K/mTOR pathway
Hongchen Zhang1,2,3, Dongchao Xu1,2,3, Yuanhui Li1,2,3 and Xiaofeng Zhang1,2,3
1Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University, Hangzhou, China;2Key Laboratory of Integrated Traditional Chinese and Western Medicine for Biliary and Pancreatic Diseases of Zhejiang Province, Hangzhou, China;3Hangzhou Institute of Digestive Disease, Hangzhou, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The DCAF13 gene, located on chromosome Xq24, encodes a protein that functions as a substrate recognition module within the CUL4-DDB1 E3 ubiquitin ligase complex. Its biological role in hepatocellular carcinoma (HCC) remains unexplored. This study aims to examine the expression of DCAF13 in HCC cell lines and its influence on the biological behavior of these cells.
Materials and Methods: The Cancer Genome Atlas (TCGA) database was utilized for bioinformatic analysis. HCC cell lines with DCAF13 overexpression and silencing were established using lentiviral constructs. Western blotting was performed to evaluate DCAF13 expression. Functional assays included the cell counting kit-8 (CCK-8) assay, EdU assay, and flow cytometry for apoptosis analysis.
Results: Bioinformatic analysis of the TCGA database indicated elevated DCAF13 expression in HCC. Overexpression and silencing experiments revealed that DCAF13 significantly influences cell proliferation and apoptosis. Functional assays demonstrated that DCAF13 promotes HCC progression by activating the AKT/PI3K/mTOR pathway, as supported by gene set enrichment analysis (GSEA).
Conclusion: Our study highlights the critical role of DCAF13 in HCC progression. DCAF13 is highly expressed in HCC and regulates cell proliferation and apoptosis. Targeting DCAF13 disrupts cell proliferation and enhances apoptosis, thereby inhibiting the growth of HCC cells.
PP-02-214
A Successful SVR After Three Regimens Of Hepatitis C
Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Airlangga University – Dr Soetomo General Academic Hospital, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Since its discovery in 1989, Hepatitis C Virus (HCV) has become cause of chronic liver disease. The evolution of HCV therapy in Indonesia follows the discovery of anti-virals in the world. Monitoring side effects and kidney function is important in the management of HCV and also how to manage HCV patients with Chronic Kidney Disease.
Case Description: A 77 years old man with Hepatitis C and Chronic Kidney Disease (eGFR 26 ml/minute/1.73 m2) was treated at Dr. Soetomo Hospital Surabaya Indonesia. In 2014, patient received dual therapy (Peg-IFN/RBV) and achieved Early Virological Response. Serious side effects (low hemoglobin level and severe thrombocytopenia appeared at week-14. Therapy was stopped at week-16. In 2015 HCV RNA was redetected. Eight years later patient received Direct Acting Antiviral (DAA) Elbasvir/Grazoprevir for 12 weeks and only achieved Delayed Virological Response. There was an increase of eGFR. Regimen was changed to Sofosbuvir alternate day (once daily) and Daclatasvir once daily. Estimeted GFR worsened after 6 weeks, although Sofosbuvir was reduced 2 times a week, then therapy was discontinued. Totally, DAA was given in 18 weeks. After stopping, HCV RNA was undetected and remained undetected after 12 weeks (SVR).
Discussion: Serious side effects such as anemia and thrombocytopenia often found when using Peg-IFN/RBV as a consequence, therapy was decided to stop and SVR could not be achieved. When using the Elbasvir/Grazoprevier regimen, no dose adjustments for eGFR < 30 ml/minute/1.73 m2. In this case there was an increase in eGFR. However, SVR was achieved after SOF-DCV.
PP-02-215
Factors associated with hepatitis B surface antigen sero-clearance in hemodialysis patients
Kiran Bajaj and Taha Raja Yaseen
Sindh institute of urology and transplantation, Karachi, Pakistan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Hemodialysis is a route to many infections with hepatitis B one of the most commonly encountered pathogen. The data regarding the factors predictive of hepatitis B surface antigen (HBsAg) clearance in hemodialysis patients is scarce. our aim was to determine the rate of hepatitis B surface antigen clearance in hemodialysis patients and also to identify the factors predictive of HBsAg clearance in this population.
Methods: It was prospective observational study which was conducted at the department of Hepatogastroenterology, Sindh Institute of Urology and Transplantation. All the patients with HBsAg reactive chronic hepatitis B undergoing hemodialysis from January 2019 to December 2020 were included in the study. Univariate followed by multivariate logistic regression analysis was performed to identify independent predictors of HBsAg loss in hemodialysis patients.
Results: A total of 213 patients with chronic HBV undergoing hemodialysis patients were included in the study. Out of them, 163(76.5%) were males. At baseline, HBsAg levels > 1000 IU/ml were noted in 159(74%) patients, HBV DNA > 5000 were observed in 109(51.1%) and HbeAg positive disease was noted in 52(24.4).Most of the patients i.e.152(71.3%) underwent single session of hemodialysis per week. HBsAg loss was noted in 26(12%) patients. On univariate analysis, HBsAg levels < 1000IU/ml, HBV DNA levels <5000IU/ml, HBeAg negative disease and multiple sessions of hemodialysis was associated with increased HBsAg loss while on multivariate analysis, HBV DNA levels of less than 5000 IU/ml at baseline and multiple sessions of hemodialysis were independent predictors of HBsAg loss in hemodialysis patients.
PP-02-216
Correlation Between Interleukin-10 Serum Levels and Liver Fibrosis Severity in Treated Chronic Hepatitis B Patients
1Department of Internal Medicine, Airlangga University Hospital, Surabaya, Indonesia;2Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This study aimed to investigate the relationship between serum IL-10 levels and liver fibrosis severity in patients with chronic hepatitis B.
Method: The population of this study is chronic hepatitis B patients who have received antiviral treatment for at least one year. We used ELISA to determine the serum IL-10 levels and Fibroscan to assess the degree of liver fibrosis, which was then categorized as mild, significant, or advanced. The Spearman correlation test and linear regression model were used to analyze the data, with the results presented as a p-value and correlation coefficient of p<0.05 considered statistically significant.
Result: With an average age of 42,69 years, the 80 participants in this study were primarily male (62,5%). The serum level of IL-10 was 10.8 (1.7-176.1) pg/ml at the median. Liver stiffness median is 6.45 ranged from 2.6-35.3 kPa. Serum levels of IL-10 and the severity of their liver fibrosis showed a significant moderate positive correlation (p<0.001; r=0,408). Linear regression model showed a 2.7 pg/mL increment of IL-10 was associated with increase 1 kPa value in liver stiffness measurement (95%CI: 1.65 - 3.79; r2 = 0.2056).
Conclusion: The results suggest that elevated serum IL-10 levels are associated with more severe liver fibrosis with in chronic hepatitis B patients treated with antiviral therapy.
PP-02-217
Chronic hepatitis b and covid-19 coinfection among admitted patients in a hospital in the philippines
Angeli Eleanor Facun, Bernard Demot and Marie Ellaine Velasquez
Baguio General Hospital and Medical Center, Baguio City, Philippines
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This paper aims to present the prevalence and outcomes of adult patients with COVID-19 and Chronic Hepatitis B coinfection at a tertiary hospital in the Philippines.
Materials and Methods: A cross-sectional descriptive study was utilized in this study.
Results: Among the 5255 Adult COVID-19 patients admitted, only 519 (10%) patients were tested for hepatitis B. Excluding those with incomplete hepatitis profile, the sample population is 311 (60%) which was classified as follows: HBeAg Positive Chronic Hepatitis B (n=0, 0%), Chronic hepatitis B with low infectivity (n=19, 6.1%), occult hepatitis B infection (n=12, 3.9%), recovered from Chronic Hepatitis B (n=60, 19.3%), vaccinated from hepatitis B (n=84, 27%) and susceptible to Chronic Hepatitis B (n=136, 43.7%). There were a total of 10 mild COVID 19 cases, 30 moderate cases, 168 severe cases and 103 critical cases. In terms of mortality rate, Chronic hepatitis B with low infectivity had 5.3%, occult hepatitis B infection had 33%, recovered from Chronic Hepatitis B had 30%, vaccinated from hepatitis B had 27.4% and susceptible to Chronic Hepatitis B had 27.2%. The average length of hospital stay was similar and lastly, in terms of complications, acute respiratory failure had the highest percentage of complication across all subgroups.
Conclusion: Chronic Hepatitis B is still identified as a disease with high burden in the Philippines. This study reflects that there really is a need to concentrate and direct more effort on these subset of vulnerable populations especially during the time of the pandemic.
PP-02-218
Reliability Of AFP Level Compared With APRI In Liver Fibrosis Prediction In Chronic Hepatitis B
Ety Febrianti1, Richard Lumbantobing1, Febry Rahmayani1, Suyata Suyata2 and Legiran Legiran3
1Internal Medicine Moh. Hoesin Hospital/Sriwijaya University, Palembang, Indonesia;2Gastroenterohepatology Division, Internal Medicine Moh. Hoesin Hospital/Sriwijaya University, Palembang, Indonesia;3Biomedical Division, Faculty of Medicine Sriwijaya University, Palembang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Chronic hepatitis B (CHB) is associated with an increased risk of liver fibrosis. Non-invasive methods such as aspartate aminotransferase to platelet ratio index (APRI) require simple laboratory values that can predict liver fibrosis in chronic hepatitis B patients. Alpha-fetoprotein (AFP) can be used indirectly as an index to indicate the stage of fibrosis in chronic hepatitis B.
Objective: This study aims to assess reliability of AFP in liver fibrosis prediction based on APRI in CHB patients at the vertical hospital Dr. Mohammad Hoesin Palembang.
Methods: This is a cross-sectional study, on 40 naive Hepatitis B virus infection patients who follow the laboratory examination at the Internal Medicine Clinic at the vertical hospital Dr. Mohammad Hoesin from January 2023 to August 2023.
Result: This research involved 40 subjects, the majority of chronic hepatitis B patients were women (57.5%) and aged 18-60 (92.5%). The results of the analysis of the AFP consistency test against APRI show a p value <0.001 and a Kappa coefficient strength of agreement value of 0.391 or fair.
Conclusion: Examination of AFP level has fair reliability and consistency with APRI in predicting liver fibrosis in chronic hepatitis B patients.
Keywords: Chronic hepatitis B, Alpha fetoprotein, Aspartate Aminotransferase to Platelet Ratio Index, Liver fibrosis.
PP-02-219
Long-term outcomes of HCC recurrence and occurrence after hepatitis C virus eradication by DAA treatment
Faculty Of Medicine, Kagawa University, Miki-cho Kagawa, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: DAA treatment (DAAt) have increased the cure rate for HCV patients, but the rates of HCC occurrence and long-term outcomes in those with HCC recurrence post-DAAt are unclear.
Methods: The study first aimed to identify predictors of HCC occurrence post-DAAt. Among 1218 HCV-infected patients, 1088 who achieved SVR and had no history of HCC treatment were included between September 2014 and November 2018.
Results: HCC incidence was 0.61%, 1.88% and 3.71% at 6, 12 and 24 months post-DAAt, respectively. Multivariate analysis revealed age (HR 1.0729, P=0.0044) and AFP levels post-DAAt (HR 1.0486, P=0.0486) as independent predictors of HCC. A scoring system (0-2 points) was developed using these factors to predict HCC occurrence. HCC incidence at 2 years was 0.3% for 0 points, 6.27% for 1 point, and 18.37% for 2 points. The second aim was to investigate recurrence rates, factors, and prognosis in 130 patients treated with DAAt after HCC treatment. HCC recurrence rates were 32.5%, 46.3%, and 59.4% at 12, 24 and 36 months, respectively. Multivariate analysis showed palliative treatment prior to DAAt (HR 3.974, 95% CI 1.924–8.207, P=0.0006) and AFP at SVR12 (HR 1.048, 95% CI 1.016–1.077, P=0.0046) were independent factors for HCC recurrence. Overall survival rates at 12, 24, and 36 months were 97.6%, 94.0%, and 89.8%, respectively.
Conclusions: Age and AFP level post-DAAt are independent predictors of HCC occurrence. This scoring system can help predict HCC risk in HCV-free patients post-DAAt. Despite recurrence rates, the prognosis improved, indicating DAAt should be considered for HCV patients.
PP-02-220
Interleukin-6 as a Biomarker for Assessing Liver Fibrosis Severity in Patients with Chronic Hepatitis B
Amal Arifi Hidayat1, Rheza Rahmadika Putra1, Ulfa Kholili2 and Ummi Maimunah2
1Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;2Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This study aimed to examine the relationship between IL-6 serum levels and the severity of liver fibrosis in patients with chronic hepatitis B.
Materials and Methods: An observational cross-sectional analytic study involving chronic hepatitis B patients who received antiviral therapy for at least 1 year. All subjects were examined for IL-6 serum levels and liver fibrosis severity. IL-6 serum levels was measured by ELISA method. Liver fibrosis severity was determined using transient elastography and categorized as follow: mild fibrosis (F0-F1), significant fibrosis (F2-F3) and advance fibrosis (F4). Data were analyzed using Kruskal-Wallis test.
Results: This study involved 78 subjects predominantly males (66.7%) with the average of age 43.05 + 10.77 years old. The median of IL-6 serum level was 6.75 (0.2-96.5) pg/ml. The median of liver stiffness was 6.35 + 8 kPa, with the proportion of mild fibrosis (16/78, 20.5%), significant fibrosis (21/78, 26.9%) and advance fibrosis (41/78, 52.5%). There was a significant association between IL-6 serum levels with liver fibrosis severity for mild, significant and advance fibrosis (4.1 pg/m vs 4.65 pg/m vs 11 pg/m, respectively; p < 0.001).
Conclusion: The findings of this study suggest that elevated IL-6 serum levels was associated with more severe liver fibrosis in patients with chronic hepatitis B.
PP-02-221
Relationship between hepatic fibrosis and several basic laboratory parameters in non-chirrotic chronic hepatitis C subjects
Boy Hutaperi1 and Putut Bayupurnama2
1Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia;2Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Hepatic fibrosis and its sequence of cirrhosis remain important problem in managing chronic hepatitis C. Unfortunately, sophisticated hepatic fibrosis assessment methods are not widely available. Requirement of basic laboratory test take place into this gap.
Objective: Evaluate correlation between hepatic fibrosis state and several basic laboratory parameters in non-chirrotic chronic hepatitis C.
Methods: Retrospective study was conducted at Sardjito hospital, enrolled 72 non-cirrhotic hepatitis C from January 2022 to February 2024. Basic laboratory measures such hemoglobin, platelet, ALT, AST, albumin and HCV-RNA level were collected. Mild and significant fibrosis group was defined by liver stiffness measurement (Fibroscan®).
Results: Among 72 non-cirrhotic hepatitis C subjects, mean age was 47.8 ± 13.2 years and 54 (74%) were men. Significant fibrosis was found in 37 (50.7%) subjects. Mann-Whitney test did not show any significant differences in terms of hemoglobin, ALT level and HCV-RNA between two group. Albumin level was higher significantly in mild fibrosis group (p = 0.032), meanwhile age and AST level were lower significantly in mild fibrosis group (p = 0.002 and p = 0.015 respectively).
Spearman correlation test revealed that age and AST level correlate positively with fibrosis state (r = 0.3; p = 0,014 and r = 0.4; p = 0.002, respectively). On contrary, hemoglobin, platelet, albumin, ALT level and HCV-RNA did not correlate positively to fibrosis.
Conclusion: Age and AST level solely had significantly positive correlation to fibrosis stage in non-chirrotic chronic hepatitis C
PP-02-222
Treatment efficacy and safety of sofosbuvir and velpastavir based treatement in Korea: Multi-institutional prospective study
Sanghyuk Jung and Ga Ram You
Chonnam National University Hospital, Gwangju, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Direct-acting antivirals (DAAs) have revolutionized Hepatitis C virus (HCV) treatment, significantly improving efficacy. The regimen of sofosbuvir, velpatasvir, and voxilaprevir has shown high efficacy across all Hepatitis C virus genotypes. This study investigates their real-world efficacy and safety profile in South Korea.
Methods: From November 2022 to January 2024, patients with HCV undergoing SOF/VEL-based treatment at six hospitals were enrolled. Patients from chronic hepatitis to decompensated liver cirrhosis, received sofosbuvir and velpatasvir (SOF/VEL) for 12 weeks. Patients who failed to achieve sustained virological response (SVR) with previous DAA treatments, from chronic hepatitis to compensated liver cirrhosis, received sofosbuvir, velpatasvir, and voxilaprevir (SOF/VEL/VOX) for 12 weeks. Exclusions included patients with hepatocellular carcinoma within six months.
Results: Among 101 patients treated with SOF/VEL, the mean age was 64.71 years, with 40.9% male. Genotype distribution was 40.6% genotype 1b and 59.4% genotype 2. The mean baseline HCV RNA level was 3,088,097 IU/mL. Underlying compensated liver cirrhosis was present in 21.8% and decompensated in 5.0%. SVR12 was achieved in 86.1% (87 patients), while 13.9% (14 patients) were lost to follow-up.
Among 17 patients on SOF/VEL/VOX, the mean age was 61.84 years, with 29.4% male. Fourteen patients had previously failed DAA treatment, one had achieved SVR, one had self-stopped DAA, and one did not check SVR. Four had compensated liver cirrhosis. SVR12 was achieved in all 16 assessable patients. No serious adverse events (≥grade 3) were reported.
Conclusion: Sofosbuvir and velpatasvir-based treatments demonstrated excellent SVR12 rates and a favorable safety profile.
PP-02-223
The effect of tenofovir alafenamide on alanine aminotransferase levels in chronic hepatitis B patients
Lilian Yan Liang1, Jimmy Che-To Lai1,2, Terry Cheuk-Fung Yip1, Vincent Wai-Sun Wong1 and Grace Lai-Hung Wong1
1Medical Data Analytic Centre (MDAC), Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, SAR, China;2Li Ka Shing Institute of Health Science, The Chinese University of Hong Kong, Hong Kong, SAR, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Tenofovir alafenamide (TAF) is known to improve alanine aminotransferase (ALT) levels and we aimed to evaluate ALT level changes in TAF-treated chronic hepatitis B (CHB) patients with or without exposure to other antiviral drugs.
Materials and methods: This is a retrospective cohort study including TAF-treated CHB patients with available ALT levels. Patients exposed to other nucleos(t)ide analogues (NAs) after starting TAF were combined with those treated only with TAF, with follow-up censored at the start of the other NAs. Those who received other NAs before TAF were classified as another group. Baseline was defined as the start date of TAF treatment.
Results: This study included 4,977 TAF-treated CHB patients, with a mean age of 61 years and 65.5% males. The corresponding ALT levels (U/L) at baseline, month 3, 6, 9, 12 were 28.8 [interquartile range: 21.0–50.0], 26.3 [19.0–36.0], 25.0 [18.0–36.0], 24.8 [17.9–35.0], 25.0 [19.0–35.0] for TAF-treated only patients and 26.0 [19.7–38.0], 24.0 [17.0–35.0], 23.0 [17.3–32.0], 23.7 [17.0–33.0], 23.0 [17.0–31.4] for TAF-treated patients exposed to other NAs (Figure). The percentages of patients with normal ALT at baseline were 77.5% and 68.6% (P < 0.05) for TAF-treated patients with and without exposure to other NAs and increased to 86.5% and 83.3% (P = 0.189) at 12 months, respectively.
Conclusion: TAF treatment reduced the ALT levels regardless of exposure to other NAs and increase the incidence of ALT normalization to comparable levels at 12 months between those with and without other NAs.
PP-02-224
Comparison One Year Treatment with Tenofovir and Adefovir in Chronic Hepatitis B Patients in Indonesia
Ummi Maimunah1, Muhammad Miftahussurur1, Firda Iragama Wessless1, Juniastuti Juniastuti2 and Yoshio Yamaoka3
1Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine - Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, Indonesia;2Department of Microbiology, Faculty of Medicine/Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, Indonesia;3Department of Enviromental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Evaluation of the Tenofovir and Adefovir as chronic hepatitis B treatment is rare in Indonesia. We evaluated Tenofovir and Adefovir therapy in chronic hepatitis B patients after one-year treatment.
Patients and methods: This study is an observational analytic study that included 74 chronic hepatitis B adult patients was determined based on the biochemical, improvement of fibrosis, and virological status.
Results: Most of the patients were HBeAg positive (43/74, 58.10%) and received treatment at 31–50 aged years (42/74, 56.75%). Significant differences in alanine aminotransferase (ALT) improvement between tenofovir-treated and adefovir-treated patients in HBeAg-positive patients (18/26, 69.23% vs. 5/17, 29.41%; P<0.05). In addition, based on the improvement of fibrosis status, there was a significant difference between tenofovir and adefovir treated patients in TE value (15/26, 57.69% vs. 8/17, 47.05%; P<0.05) and degree of fibrosis (10/26, 38.46% vs. 8/17, 47.05%; P<0.05) both in the HBeAg-positive and negative groups. Furthermore, based on virological status among patients receiving tenofovir, there was a significant difference in median HBV DNA between HBeAg-positive and HBeAg-negative groups before and after one year of treatment (1.7×106IU/ml vs.7.1×107IU/ml; P<0.05) and (6.5×105IU/ml vs. 2.8×106IU/ml; P<0.05, respectively). A significant difference in median value reduction was also found between tenofovir and adefovir patients in the HBeAg-positive group (1.08×108IU/ml vs. 3.9×107IU/ml; P<0.05).
Conclusion: After one year of treatment, tenofovir was advanced to reducing the virus and improving ALT and fibrosis compared to adefovir.
PP-02-225
Elevated Serum TNF-α Levels Correlate with Increased Liver Fibrosis in Treated Chronic Hepatitis B Patients
Ummi Maimunah1, Dio Brimantyo2 and Ulfa Kholili1
1Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia;2Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This study aiming to analyze the correlation between serum TNF-α level and degree of liver fibrosis in chronic hepatitis B patients in the Gastroenterohepatology Outpatient Installation at Dr. Soetomo Hospital Surabaya.
Methods: This was a cross-sectional study including 82 treated chronic hepatitis B subjects. TNF-α serum levels were measured using specific monoclonal antibodies via a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kit. Transient elastography result was classified according to METAVIR score, F0, F1, F2, F3, and F4. Data was analyzed by the Spearman correlation test and liner with a p<0.05 was considered statistically significant.
Results: The study included 82 predominantly male participants, with an average age of 42.22 years. The median serum TNF-α level was 8.70 pg/mL, ranging from 0.40 pg/mL to 312.40 pg/mL. The median liver stiffness, a measure of fibrosis, was 6.35 kPa, with values ranging from 2.6 kPa to 27.6 kPa. There was significant association between TNF-α value with the METAVIR score (p < 0.01). Regression model showed increased TNF-α value of 19.9 in each increase fibrosis stage (95%CI = 10.32 - 29.64, p < 0.001)
Conclusion: The findings of this study suggest that higher serum TNF-α levels are associated with increased liver fibrosis in chronic hepatitis B patients
PP-02-226
SCFA and Butyrate Level Relationship with APRI and FIB-4 Score in Hepatitis B or C
Syifa Mustika1, Mochamad Fachrureza1, Iraky Mardya Rakhmadhan2, Muhammad Reyhan2 and Adam Adam2
1Staff of Gastroentero-Hepatology Division, Internal Medicine Department, Dr. Saiful Anwar General Hospital, Malang, Indonesia;2Indonesian Society of Internal Medicine Resident, University of Brawijaya, Malang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The study investigated the relationship between SCFA and butyrate acid levels and APRI and FIB-4 scores in hepatitis B and C-related cirrhosis.
Materials and Methods: This cross-sectional study included 27 fecal samples from patients with cirrhosis related to hepatitis B and C in the gastroentero-hepatology clinic at Saiful Anwar General Hospital in Malang, East Java, where SCFA and butyrate acid levels were measured using a gas chromatography analyzer.
Results: The study analyzed fecal SCFA levels among Hepatitis B and C patients using APRI scores between low-risk and intermediate-risk groups (9.18 ± 4.2 vs 12.40 ± 6.2, p=0.172), as well as butyrate levels (1.45 ± 1.09 vs 2.70 ± 1.88, p=0.056). In addition, the patients were categorized into low, intermediate, and high-risk groups based on their FIB-4 scores. However, there were no significant differences in butyrate levels (1.55 ± 1.18 vs 1.10 ± 0.28 vs 3.02 ± 2.01, p=0.067) or average fecal SCFA levels (9.6 ± 4.53 vs 7.80 ± 3.27 vs 12.75 ± 6.55, p=0.30) between these groups. The analysis suggests that fecal SCFA levels may vary among different groups of patients.
Conclusion: The study found that liver fibrosis stages in hepatitis B and C patients were not significantly correlated by fecal SCFA and butyrate levels, possibly due to confounding factors like diets and medications history that were not being measured.
PP-02-227
Efficacy and safety of glucocorticoids therapy for severe fulminant viral hepatitis: a meta-analysis
Kailash Mani Pokhrel and Kapil Khanal
Maharajgunj Medical Campus, Kathmandu, Nepal
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Severe fulminant viral hepatitis is associated with high mortality rates. The effectiveness of glucocorticoid therapy in this condition is debated across studies. Therefore, we conducted this meta-analysis to access the efficacy and safety of glucocorticoid therapy for severe fulminant viral hepatitis.
Materials and methods: Adhering to PRISMA guidelines, we systematically searched Embase, PubMed, Scopus, and Google Scholar using specific search strategies for studies published in English up to July 9, 2024, comparing glucocorticoids with non-glucocorticoids treatments. The primary outcome was reduction in mortality rate, and secondary outcomes included improvement in liver function parameters and complications associated with glucocorticoid treatment.
Results: Our meta-analysis included four randomized trials and 12 cohort studies, involving 2972 patients. Mortality was significantly lower in glucocorticoid treatment group compared to non-glucocorticoid group [Odds ratio (OR) = 0.41, 95% confidence interval (CI) 0.21 – 0.80, p = 0.009]. There was no significant difference in standardized mean differences (SMD) of total bilirubin and albumin levels between corticosteroid group and non-corticosteroid group before and after treatment. Bleeding [OR = 1.54, 95%CI 0.69 – 3.46, p = 0.29] and infection rates [ OR = 1.63, 95% CI 0.57 – 4.69, p = 0.36] were higher in the corticosteroid group, but these differences were not statistically significant.
Conclusion: Glucocorticoid therapy for severe fulminant viral hepatitis significantly reduces the mortality without significant increase in bleeding or infection rates.
PP-02-228
Meta-analysis of hbv vaccine response in hemodialysis patients: impact of diabetes mellitus and hemoglobin level
Edgar Irvin Raranta1, Bradley Jimmy Waleleng2, Fandy Gosal2, Luciana Rotty2, Jeanne Winarta2 and Andrew Waleleng2
1Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;2Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This meta-analysis aims to assess and synthesize the existing evidence regarding the Hepatitis B Virus (HBV) vaccine response in hemodialysis patients, focusing on the impact of comorbid diabetes mellitus and hemoglobin levels.
Methods: A comprehensive review of relevant studies was conducted from online databases PubMed, EMBASE, the Cochrane Library, and Google Scholar using terms like "Hepatitis B vaccine", “Response”, "Diabetes mellitus", and "Hemoglobin" for studies published up to August 2024. Duplicates were removed, and titles, abstracts, and full texts were screened for relevance. The review included studies that reported the impact of DM and hemoglobin levels on HBV vaccine response.
Results: Out of 183 identified articles, 57 were screened and 40 were deemed relevant. We identified 8 studies involving 4,377 patients on HD. The findings indicate that patients with DM exhibit a significantly lower seroconversion rate compared to non-diabetic patients, highlighting the adverse effect of this comorbidity on HBV vaccine response. Additionally, patients with higher hemoglobin levels demonstrated improved seroconversion rates, suggesting that adequate hemoglobin may enhance immune response to the vaccine. Aggregation of study results showed a significant decrease in response rates among the diabetic patients [pooled OR=0.59 (95% CI 0.50-0.70)] and higher hemoglobin levels in responders [mean difference 0.40 (95%CI 0,25-0.54].
Conclusion: Our meta-analysis showed a significant association between diabetes mellitus and hemoglobin levels with impaired response to HBV vaccine in HD patients. Future research should prioritize exploring optimized vaccination protocols and management of comorbidities to enhance protective immunity against HBV in HD patients.
PP-02-229
Association between vitamin d level and hepatitis b disease: a systematic review
Edgar Irvin Raranta1, Bradley Jimmy Waleleng2, Fandy Gosal2, Luciana Rotty2, Jeanne Winarta2 and Andrew Waleleng2
1Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia;2Gastrohepatology Division, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University/Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: This systematic review aims to assess and synthesize the existing evidence regarding the relationship between vitamin D levels and clinical conditions in patients with hepatitis B.
Methods: Following PRISMA guidelines, this review conducted a thorough search of PubMed, EMBASE, the Cochrane Library, and Google Scholar using terms like "Hepatitis B," "Vitamin D," and "Liver Disease" for studies published up to August 2024. Duplicates were removed, and titles, abstracts, and full texts were screened for relevance. The review included randomized controlled trials (RCTs) that reported serum vitamin D levels and their effects on HBV outcomes.
Results: Out of 214 identified articles, 148 were refined to those with free full-text access, and 100 were deemed relevant. Five high-quality RCTs, published between 2014 and 2024, were included. These studies, involving diverse populations such as infants, chronic hepatitis B (CHB) patients, and HIV-infected adults, used various vitamin D measurement methods, mainly focusing on serum 25-hydroxyvitamin D (25(OH)D). The findings showed mixed effects of vitamin D on HBV outcomes, with some studies indicating no significant impact on HBV replication or vaccine response, while others highlighted the importance of adequate vitamin D levels before vaccination for optimal vaccine efficacy.
Conclusion: The evidence regarding vitamin D and HBV infection is inconclusive. While some data suggest potential benefits, such as improved vaccine efficacy and immune modulation, other studies report no significant effects. Further research is needed to resolve these inconsistencies and determine the association betweel vitamin D and Hepatitis B.
PP-02-231
Acute Kidney Injury in Acute-on-Chronic Liver failure: Prognostic indicators and Clinical outcomes
Sumaswi Angadi1, Srujan Reddy1, Suprabhat Giri2 and Sukanya Bhrugumalla1
1Nizam's Institute of Medical Sciences, Hyderabad, India;2Kalinga Institute of Medical Sciences, Bhubaneswar, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The present study aims to analyze the progression of AKI in ACLF and identify predictive factors for mortality.
Materials and Methods: This is a single-center, retrospective analysis of patients with ACLF and AKI admitted to a tertiary center in South India between January 2023 and April 2024. The diagnosis of ACLF was based on the Asia Pacific Association for the Study of Liver (APASL) definition. Clinical outcomes at 28 days and 90 days were recorded. Factors predicting mortality were analyzed using Receiver Operating Characteristic (ROC) curves. Survival analysis was performed using Kaplan-Meier and Cox regression models.
Results: A total of 102 patients with ACLF and AKI were included in the study. In-hospital mortality was 36.27%, while mortality at 28 days was 38.24%, and at 90 days was 49.02%. Hepatorenal syndrome (HRS)- acute kidney disease (AKD) developed in 9.8%, and 1.96% developed HRS- chronic kidney disease (CKD). Non-survivors had a significantly higher APASL Research Consortium Score (AARC) score (p<0.001) and a higher stage of AKI compared to survivors (p<0.001). Also, HRS was common among survivors compared to Inflammation/sepsis-associated AKI among non-survivors (p=0.001). The AARC score had a high AUROC of 0.907, followed by the MELD score (AUROC:0.872), serum urea (AUROC:0.709), creatinine (AUROC:0.704) and bilirubin (AUROC: 0.697). Independent predictors of mortality were AARC score (HR:1.759, CI:1.331-2.325, p <0.001) and serum urea (HR:1.009, CI:1.002 - 1.016, p=0.016).
Conclusion: AARC score and serum urea are better mortality predictors than serum creatinine, serum bilirubin, and MELD scores. Inflammation/Sepsis-associated AKI is common among non-survivors.
PP-02-232
Plasma Exchange for Acute-on-Chronic Liver Failure from Severe Alcoholic Hepatitis with Failed Steroid Therapy
Nagarajan Venkatchocku Ashok Annamalai1 and Marianne Anastasia De Roza2
1Yong Loo Lin School of Medicine, National University of Singapore, Singapore;2Department of Gastroenterology and Hepatology, Sengkang General Hospital, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Acute-on-chronic liver failure (ACLF) associated with multi-organ failure, has a high mortality burden. The mechanisms are thought to be driven by hyperactive inflammatory and immune responses triggered by microbial and non-microbial factors. Plasma exchange (PLEX) is a potential alternative to liver transplant that improves survival.
Case Report: A 56-year-old man with Child-Pugh C11 alcoholic liver cirrhosis was admitted to the intensive care unit for Grade 3 ACLF, precipitated by severe alcoholic hepatitis. (Albumin 29 Bilirubin 414 ALP 133 ALT 40 AST 128 Na 123 Creatinine 487 INR 1.82) with MELD score of 39. He required inotropic support and renal replacement therapy(RRT).
Patient was covered with antibiotics and started on steroids for Maddrey Discriminant Function (MDF) score of 61.5. However, there was no improvement with Lille score 0.603 (>0.45) on day 5 and 0.535 on day 7, hence steroids were discontinued. Patient was not a liver transplant candidate due to alcoholism and poor social support. He was commenced on a trial of PLEX and completed 3 cycles with standard volume plasma replacement resulting in clinical and biochemical improvement. He was discharged from hospital with 30-day survival at outpatient review.
Discussion: In summary, this highlights that PLEX is a potential treatment option in Alcoholic ACLF patients which would otherwise have a 1 month mortality of 74%, as it reduces systemic inflammatory reactions & modulates the adaptive immune response. This is especially important in view of Singapore’s organ scarcity and the limited transplant options for those without social support.
PP-02-233
Ancient Remedies, Modern Risks: Case Series of Acute Liver Failure Due to Traditional Complementary Medicine
Wah Loong Chan, Yee Ling Tan, Kee Huat Chuah, Stanley Khoo and Wah Kheong Chan
University Of Malaya, Kuala Lumpur, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Traditional Complementary Medicine (TCM) is widely used in Asia and can lead to fulminant liver failure. This case series highlights severe hepatotoxicity associated with TCM use, even in patients without pre-existing liver disease, and emphasizes the importance of thorough medication history in evaluating unexplained liver injury.
Case series: Case 1: A 41-year-old Thai woman with no past medical history developed acute liver failure (ALF) with elevated liver enzymes (AST 9660 U/L, ALT 1438 U/L), hyperbilirubinemia (273 μmol/L), hyperlactatemia (4.10 mmol/L), coagulopathy (INR 1.9), and hepatic encephalopathy after consuming TCM containing Borneolum Syntheticum for three months. Despite intensive care including N-acetylcysteine and plasma exchange (PLEX), she succumbed to complications as liver transplantation was not an option for her.
Case 2: A 30-year-old healthy woman presented with ALF (AST 15,210 U/L, ALT 9,520 U/L), hyperbilirubinemia (463 μmol/L), and hepatic encephalopathy after taking unspecified TCM for three months. She fully recovered after two cycles of low-volume PLEX without needing liver transplantation.
Discussion and conclusion: Both cases demonstrate severe hepatotoxicity associated with TCM use. TCM-induced liver injury can present as hepatocellular, cholestatic, or mixed patterns. This report emphasizes the importance of detailed medication history, including TCM, in evaluating unexplained liver injury. Increased awareness of TCM hepatotoxicity is necessary among practitioners and the public. Although both cases met the King's College Criteria for ALF, liver transplantation was limited. PLEX can be a bridging and definitive rescue therapy for selected ALF patients.
PP-02-234
Successful Treatment of Acute Liver Failure with Conservative Therapy in Young Adults
Putri Nurra Hakim and Bella Monika Rajagukguk and Muhammad Yugo Hario Sakti Dua and Shinta Vera Renata Hutajulu and Franz Josef Vincentius Pangalila
Mayapada Hospital Jakarta Selatan, Jakarta, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Acute Liver failure (ALF) is a rapid loss of liver function that occurs in days or weeks without pre-existing liver disease. It can be caused by medications, autoimmune disease, metabolic disease, hepatitis and other viruses. This report presents the case of an 18-year-old female with acute liver failure following tuberculosis treatment and the role of supportive therapies in managing ALF.
Case Report: An 18-year-old female presented to the emergency room with hepatic encephalopathy. Patient showed agitation, respiratory distress and decreased consciousness and was then transferred to the intensive care unit to be intubated. Patient had a history of lymphadenitis tuberculosis with treatment regimen of Rifampicin and Pyrazinamide. Diagnostic workup revealed very high level of AST and ALT, bilirubin, amylase, ammonia, and a reactive result of CMV IgG. Hepatitis C and hepatitis B marker were found to be non-reactive. Patient was given antibiotics and administration of L-ornithine-L-aspartate (LOLA), that reduce ammonia levels and improve mental status (GCS), and Stronger Neo-Minophagen C (SNMC), which provides a hepatoprotective effect and reduce ALT and AST levels. Patient showed good response with therapy, regained consciousness and extubated after four days. Patient was discharged to the ward the following day.
Discussion: This case highlights the use of appropriate and prompt supportive therapies in managing ALF and the effectiveness of non-surgical approaches in stabilizing patients without invasive intervention. This report also indicates the crucial role of early recognition, prompt initiation of appropriate treatments and close monitoring in improving clinical outcomes for patients with acute liver failure.
PP-02-235
The Application of a kind of ROS-Sensitive Nanoparticles in the Treatment of Acute Liver Injury
ZiYi Peng
Department Of Gastroenterology And Hepatology, Tianjin Medical University, Tianjin, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Acetaminophen (APAP),which is widely used all over the world and also prone to overdose, is the leading cause of acute liver injury(ALF).Meanwhile,reactive oxygen species (ROS) plays crucial role in the mechanisms of APAP-induced liver injury (AILI).Restoring liver functions to relieve acute liver injury by cutting off ROS overproduction is a potential strategy for ALF treatment.N-acetylcysteine (NAC) has been the only clinically available antidote but its therapeutic window is quite limited.
Therefore,we design a kind of single atom nanoparticles which almost accumulate in the liver and exert reactive oxygen species scavenging function.
Moreover,this kind of ROS-Sensitive nanoparticles also plays a positive role in responding to inflammation,cell apoptosis, mitochondrial damage,and regenerative disorders studied in vivo and in vitro,which are other relative mechanisms mediating ALF.Considering the superior performance of combing nanomedicine and hepatology, SeNPs therapeutic effect and good biocompatibility may provide an effective alternative for further clinical treatment of AILI.
PP-02-236
The Effectiveness of Heparin in Preventing Liver Failure in Post-Hepatectomy Patients: An Evidence-Based Case Report
Rizki Trismimanda1, Fhathia Avisha2, Crisdina Suseno3 and Masyfuk Zuhdi Jamhur4
1General Practitioner, Kudungga District Hospital, Kutai Timur, Indonesia;2Department of Internal Medicine, Ciptomangukusumo Hospital, Jakarta, Indonesia;3General Practitioner, Mentawai Island District Hospital, Mentawai Island Regency, Indonesia;4General Practitioner, Medical Faculty of Andalas University, Padang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Post-hepatectomy liver failure (PHLF) is a significant complication of hepatectomy procedure in the first few days after surgery. Heparin is an anticoagulant that protects endothelial cells, prevents thrombosis in hepatic vessels, reduces hepatic hemodynamic abnormalities, and significantly decreases hepatocyte apoptosis due to hemorrhagic and reperfusion injuries. However, the effectiveness of using heparin as a prophylactic therapy in liver surgery remains debated and requires further research.
Objective: To determine the effectiveness of heparin in preventing liver failure after hepatectomy or liver resection.
Methods: A literature search was conducted on four databases, namely PubMed, Cochrane, Scopus, and ScienceDirect on June 27, 2024, using the keywords “Heparin” OR “Unfractionated heparin” OR “Anticoagulant” AND “Post Hepatectomy” OR “Liver Resection” AND “Liver Failure”. There were 0 studies on PubMed and Cochrane, 1 study on Scopus, and 9 on ScienceDirect. The articles found were screened based on the inclusion and exclusion criteria. Critical reviews were conducted using the Critical Appraisal Skills Programme for Cohort Studies.
Results: Heparin showed a significant association with a decreased risk of PHLF (OR: 0.518; 95% CI: 0.295-0.910; P=0.022). Furthermore, heparin therapy was linked to improved short-term postoperative outcomes, such as shorter ICU stays, decreased need for respiratory support and CRRT, lower occurrences of hypoxemia, and ICU mortality.
Conclusion: Heparin administration in post-hepatectomy patients decreases the occurrence of liver failure and improves short-term postoperative outcomes. Heparin therapy may be a valuable prophylactic treatment for preventing PHLF in post-hepatectomy patients.
Keywords: Hepatectomy, Heparin, Liver Resection, Post Hepatectomy Liver Failure
PP-02-237
Decoding the Crosstalk: Neutrophil Extracellular Traps and CD8+ T Cells in Autoimmune Hepatitis
Shuhui Wang, Yu Chen, Yu Lei, Dean Tian and Han Wang
Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: CD8+ T cells cluster in the liver during autoimmune hepatitis, leading to ongoing disease and relapse after treatment stops. High levels of neutrophil extracellular traps are found in the livers of AIH patients and models, but their relationship with CD8+ T cells remains unclear.
Materials and Methods: The chronic AIH mouse model was developed and progressed through infection of AAV9-CYP2D6. Depletion of NETs was accomplished by utilizing PAD4 knockout (KO) mice. The mechanism by which NETs regulate the functions of CD8+ T cells was explored through RNA sequencing. Flow cytometry and immunofluorescence analyses were conducted to investigate the interaction between NETs and CD8+ T cells.
Results: In the context of AIH in the liver, there was a positive correlation between the presence of NETs and CD8+ T cells. Reduction of NETs was found to impede the progression of AIH and the infiltration of CD8+ T cells. Analysis of RNA sequencing data indicated that NETs influenced the mobility and cytotoxicity of CD8+ T cells. Flow cytometry analysis further confirmed that NETs hindered the migration and cytokine-mediated cytotoxicity of CD8+ T cells, while also entraped these cells. In vivo experiments with PAD4 KO mice showed that inhibiting NETs reduced the cytotoxic activity of CD8+ T cells.
Conclusion: NETs can drive AIH progression by entrapping and affecting CD8+ T cells, leading to increased cytotoxic activity and promoting crosstalk between innate and adaptive immunity in the liver. Targeting NETs and CD8+ T cell interactions may be a promising treatment approach for AIH.
PP-02-238
Imaging findings of massive splenic infarction that occurred before liver transplantation: a case report
Ki Choon Sim, Min Ju Kim, Beom Jin Park, Deuk Jae Sung, Na Yeon Han and Yeo Eun Han
Department of Radiology, Korea University Anam Hospital, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Splenic infarction, often linked to hemato-oncological, infectious, immunological conditions, or ischemia, occurs when the spleen's arterial or venous circulation is compromised. We present the case of a 56-year-old male with alcoholic liver cirrhosis who developed massive intraabdominal bleeding and hepatorenal syndrome preoperatively, and successfully underwent liver transplantation (LT).
Case Description: The patient was transferred to our tertiary institution due to worsening refractory ascites and recently developed intraabdominal bleeding. A CT scan confirmed hemoperitoneum, and successful embolization was performed on the bleeding cystic artery branch. Retrospective CT review showed a large, indistinct low-density area in the spleen, but this was initially missed. However, no physician, including the radiologist, was aware of the spleen issue at that time. Subsequently, living donor LT was performed using a modified right lobe graft of the orthotopic standard type, and splenic artery ligation was not performed. On postoperative day 3, a CT follow-up revealed a massive splenic infarction was recognized for the first time on imaging. Subsequently, complicated fluid collection that could not be controlled with conservative treatment continued in the perisplenic area. Therefore, an exploratory laparotomy, including splenectomy, was performed 48 days after the LT. Histopathology showed the spleen was hemorrhagic and necrotic appearance with cystic change, consistent with splenic infarction.
Discussion: We hypothesize that the massive splenic infarction most likely resulted from hypoperfusion and a hypercoagulable state before liver transplantation, and authors believe it had a negative impact on the patient's recovery after the liver transplantation.
PP-02-239
Characteristics of patients referred to a specialized fatty liver clinic in Abu Dhabi
Heba Abualkas and Mahmoud Kiblawi and Wala Hamed and Omar Khaddam and Ahmad Alrifai
Sheikh Shakhbout Medical City Ssmc, Abudhabi, United Arab Emirates
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To highlight the characteristics of the patients referred to the first specialized clinic for steatotic liver disease in Abu Dhabi, Sheikh Shakhbout Medical City during the period between 2018 to 2020.
Method: This is a retrospective cohort study on patients referred to fatty liver clinic with abdominal ultrasound showing fatty liver. All data were obtained from the electronic outpatient records. Individual data was collected for patients including comorbidities, relevant laboratory values amongst others. All patients had assessment for fibrosis by usual scores and VCTE( vibration controlled transient elastography).
Result: A total of 444 patients were included in the study with male representing 39.4%. we found that 40.% were diabetic, 65% patients were obese , 25 % were hypertensive, 44 % has dyslipidemia and 3.4% had cardiovascular disease. 28% of patients has more than1component of metabolic syndrome. 0.9% on presentation had NASH cirrhosis. using fib4 score17% of the patients were consider to have significant fibrosis (FIB 4>1.4). All patients had liver stiffness measurement using VCTE. 13.3% of the patients were classified as significant fibrosis (LS >8 KPA) of them 53% diabetic and 31% obese.
Conclusion: Our cohort showed that majority of patients attending the fatty liver clinic fulfill the criteria of MASLD( metabolic dysfunction associated liver disease). Significant percentage of them had advanced fibrosis on presentation and 1 % already cirrhotic. Patients with advanced fibrosis has high prevalence of diabetes and obesity and these findings support screening in this high risk population.
PP-02-240
Characteristics of Metabolic Dysfunction Associated Steatotic Liver Disease patients with advanced fibrosis assessed by fibroscan
Heba Abualkas and Mahmoud Kiblawi and Wala Hamed and Omar Khaddam and Ahmad Alrifai
Sheikh Shakhbout Medical City SSMC, Abudhabi, United Arab Emirates
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To study the characteristics of patients who underwent a vibration controlled transient elastography (VCTE) fibroscan assessment in fatty liver clinic. The goal is to compare patients with an advanced fibrosis score of F3–F4 with the group of patients with a score of F0–F2.
Methods: This is a retrospective review of the electronic medical records of adult patients who had fibroscan during 2018 to 2020, total of 399. we looked at various variables including demographics, comorbidities, and relevant laboratory values amongst others. Wilcoxon rank sum test was used to compared continuous and ordered data. Fisher’s exact test was used to compare categorical data.
Results: The study included 399 patients who underwent a VCTE assessment. Patients with advanced fibrosis (F3-F4) were 7.5% of the total study population. The median age of patients with score of F3-F4 was 52 years of age as compared to patients with score of F0-F2 which was 47, this difference was statistically significant (p=0.034). Patients classified as F3-F4 on Fibroscan were more likely to have dyslipidemia, diabetic, and obese. Moreover they tend to have cirrhosis on ultrasound, higher Non Alcoholic Fatty Liver Disease (NAFLD) score, higher Fibrosis Index Based on 4 factors (FIB-4) score, and higher Aspartate Aminotransferase to Platelet Ratio Index (APRI) score.
Conclusion: Patients with features of metabolic syndrome and with high fibrosis score tend to have advanced fibrosis in our studied population. This highlights the importance of early screening and implementation of prevention strategies in our population to prevent disease progression.
PP-02-241
Risk of liver fibrosis in patients on methotrexate with coexisting metabolic and other risk factors
Shiran Appuhamy1, Chamila Ranawaka1, Suranjan Viraj1, Sakshara Ranasinghe1, D M Amaratunga1 and Duminda Munidasa2
1Colombo North Teaching Hospital, Ragama, Sri Lanka;2Rheumatology and Rehabilitation Hospital, Ragama, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The study aims to evaluate the risk of liver fibrosis in patients undergoing long-term Methotrexate (MTX) therapy for rheumatoid arthritis (RA) and psoriasis, considering the influence of metabolic and other risk factors including diabetes mellitus, hypertension, dyslipidaemia, alcohol use, and Ayurvedic medication use.
Materials and Methods: We analysed clinical data from 206 patients on MTX for rheumatoid arthritis or psoriasis for more than 6 months. Stage of liver fibrosis was assessed by transient elastography (fibroscan). Ordinal regression and correlation analyses were performed to investigate the relationships between liver fibrosis and the specified risk factors.
Results: Descriptive statistics revealed that 73.8% of patients were on MTX therapy, with 78.2% having RA and 21.8% having psoriasis. The ordinal regression analysis indicated that MTX use is associated with a lower risk of advanced liver fibrosis stages (coefficients ranging from -0.354 to -0.163 across thresholds). Correlation analysis showed a weak negative correlation between MTX use and liver fibrosis (r = -0.108). Positive correlations were observed between liver fibrosis and alcohol use (r = 0.225), diabetes (r = 0.231), hypertension (r = 0.131), and dyslipidaemia (r = 0.139).
Conclusion: The study suggests that long-term MTX use in patients with RA or psoriasis is not associated with a significant risk of advanced liver fibrosis. However, coexisting metabolic conditions and alcohol use, significantly increase the risk of liver fibrosis. These findings underscore the importance of monitoring liver health and managing metabolic risk factors in patients undergoing MTX therapy for RA and Psoriasis.
PP-02-242
Factors Influencing Liver Steatosis and Fibrosis: A Descriptive and Regression Analysis
Shiran Appuhamy, Chamila Ranawaka, Suranjan Viraj and Sakshara Ranasinghe
Colombo North Teaching Hospital, Ragama, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to identify the factors influencing liver steatosis and fibrosis, focusing on age, gender, BMI, alcohol use, Ayurvedic medication history, diabetes mellitus, hypertension, and dyslipidaemia.
Materials and Methods: Data, including demographic and clinical variables were collected from 204 patients who underwent transient elastography (fibroscan) and analysed. Steatosis and fibrosis stages were classified using established criteria. Regression analysis was performed to determine the relationship between selected factors and liver conditions. Descriptive statistics and correlation analyses were used to elucidate gender differences and the impact of BMI and age.
Results: Descriptive analysis showed a median age of 55 years and a mean BMI of 25.31. Males comprised 60.29% of the cohort. The most severe steatosis stage (S3) was more prevalent in males (47.15%) compared to females (43.21%). Advanced fibrosis (cirrhosis) was slightly higher in males (15.45%) than females (12.35%). The regression analysis revealed that BMI (p<0.001) and dyslipidaemia (p=0.039) positively influenced liver steatosis. The model explained 16.1% of the variance (R²=0.161). For fibrosis, diabetes (p=0.005) was positively associated, explaining 12.7% of the variance (R²=0.127).
Conclusion: BMI and dyslipidaemia significantly influence liver steatosis, while DM is a key factor in liver fibrosis. The study highlights the importance of implementing lifestyle and medical interventions focusing on weight reduction, diabetes control and management of dyslipidaemia to mitigate liver disease progression.
PP-02-243
Prevalence of liver fibrosis in patients with ultrasound-proven fatty liver disease and chronic liver disease
Shiran Appuhamy, Chamila Ranawaka, Suranjan Viraj and Sakshara Ranasinghe
Colombo North Teaching Hospital, Ragama, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to assess the prevalence and severity of liver fibrosis in patients with ultrasound evidence of either Fatty Liver Disease (FLD) or Chronic Liver Disease (CLD).
Materials and Methods: We evaluated 1533 patients with ultrasound-diagnosed FLD or early CLD by using transient elastography (fibroscan) from October 2021 to May 2024. Data were analysed to determine the distribution of steatosis and fibrosis stages in both patient groups. Steatosis and fibrosis stages were classified using established criteria.
Results: Among FLD patients, 56.04% had severe steatosis (S3), 19.04% had moderate steatosis (S2), and 15.07% had no steatosis (S0). In CLD patients, 43.24% had no steatosis (S0), 24.32% had severe steatosis (S3), and 23.42% had moderate steatosis (S2). Considering fibrosis, 39.98% of FLD patients had no fibrosis (F0), 21.61% at F2, and 14.98% at F3. In CLD patients, 53.15% found to have advanced cirrhosis, 17.12% at F4, and 12.61% at F3.
Conclusion: The findings reveal a significant prevalence of severe steatosis in patients with ultrasound-diagnosed FLD. Many FLD patients remain at early fibrosis stages (F0), yet a notable proportion show significant fibrosis (F2 and F3) highlighting the need for early detection and management to prevent progression. In contrast, CLD patients exhibit a high prevalence of advanced fibrosis and cirrhosis, reflecting the chronic and progressive nature of the disease. Monitoring of patients with ultrasound evidence FLD or CLD using transient elastography is pivotal for early fibrosis detection, enabling better disease management and prevention of progression cirrhosis.
PP-02-244
Performance of FibroTouch® in Assessing Hepatic Steatosis and Fibrosis for Metabolic Dysfunction-Associated Steatotic Liver Disease
Roongruedee Chaiteerakij and Soe Thiha Maung and Thanikan Sukaram and Yuda Chongpison and Chonlada Phathong
Roongruedee Chaiteerakij, Associate Professor, Chulalongkorn University, Bangkok, Thailand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: FibroTouch® has shown efficacy in staging hepatic fibrosis in patients with chronic viral hepatitis B, but its performance in assessing liver steatosis and fibrosis in metabolic dysfunction-associated steatotic liver disease (MASLD) patients remains understudied. We aimed to evaluate the diagnostic performance of FibroTouch® in assessing steatosis and fibrosis in MASLD population.
Materials and Methods: Liver stiffness measurements and steatosis were assessed using FibroTouch® and FibroScan®, with FibroScan® as the reference standard. Pearson’s correlation test evaluated correlations, and kappa statistics determined agreement between two methods. Optimal cut-off values of FibroTouch® for predicting hepatic steatosis and fibrosis stages were determined through ROC curve analysis, with Youden index method.
Results: Strong correlations were observed between FibroTouch® UAP and FibroScan® CAP (rho=0.74) and LSM values (rho=0.87) (p<0.001 for both) in total 380 patients. The mean CAP value for the entire cohort was 285±51 dB/m, and the median LSM for the cohort was 5.3kPa. The optimal FibroTouch® UAP cutoffs were 229 dB/m for S0 vs. S1, 267 dB/m for S1 vs. S2, and 294 dB/m for S2 vs. S3. For FibroTouch® LSM, the optimal cutoffs were 6.0kPa for F0-F1 vs. F2, 7.9kPa for F2 vs. F3, and 10.6kPa for F3 vs. F4. Moreover, FibroTouch® effectively assessed hepatic steatosis and fibrosis in patients with different BMIs.
Conclusion: FibroTouch® proved valuable in assessing hepatic steatosis and liver fibrosis staging in MASLD patients, enhancing its applicability in various clinical settings as a suitable and convenient option for MASLD patients.
PP-02-245
Phenotyping of patients admitted in the past year in a colombian referral center in ibd
Viviana Parra-izquierdo and Ginary Orduz and Silvia Hhoyos and Andrea Reatiga and Oscar Pinto and Juliep Sarmiento and Carlos Cuadros and Oscar Pinto and Alexis Duarte and Johon Garces and Juan Acevedo and Juan Frias and Cristian Florez
Leuven University, Leuven, Belgium
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: In Colombia there are currently 5 centers of excellence certified by the Pan American Crohn's and Colitis Organization and one of them is the only one that is certified and serves the population of eastern Colombia.
Objective: Description of the cohort of patients who have been admitted in the last year to a Colombian Center of of Excellence in Inflammatory Bowel Disease.
Materials and Methods: Descriptive observational cross-sectional study of patients diagnosed with pediatric and adult diagnosed pediatric and adult patients with Inflammatory Bowel Disease who have been admitted to a Center of Excellence.
Results: Within the cohort of patients of the Center of Excellence in Inflammatory Bowel Disease, there are 90 patients, 56.6% of whom are women. Intestinal Disease, there are 90 patients of which 56.6% are omen. The average age is 40.8 years, with a minimum age of 7 years and a maximum age of 80 years, with 10 patients of pediatric age. The 68.8% are Ulcerative Colitis (37% pancolitis, 54.8% left colitis and 8% proctitis) and 31.1% Crohn's Disease (64.2% ileal, 28.5% ileocolonic and 7.14% colonic) with 17.85% with fistulizing and stenosing involvement. In Ulcerative Colitis 27.41% are receiving biologic therapy, infliximab being the most used biologic 47%, adalimumab 23.5%, vedolizumab 23.5% and 5.8% golimumab 5.8%.
Conclusions: This study provides valuable information on inflammatory bowel disease in eastern Colombia, showing that there is a predominance of ulcerative colitis with greater use of biologic therapy
PP-02-246
TrkB mitigated liver fibrosis through regulating macrophage recruitment mediated by the TGF-β/c-Fos/CCL2 axis
1Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, China;2Shanghai Institute of Liver Diseases, Shanghai, China;3Joint Laboratory of Biomaterials and Translational Medicine, Puheng Technology, Suzhou, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Liver fibrosis is a main indicator for increased mortality and long-term comorbidity in nonalcoholic steatohepatitis. Our prior research has established that TrkB overexpression in hepatic stellate cells inhibited the TGFβ/SMAD signaling and alleviated hepatic fibrosis. This study aimed to delineate the role of TrkB overexpression in hepatocytes for liver fibrosis.
Materials and Methods: Using the GEO database and liver samples with liver fibrosis, we investigated the expression of TrkB in different stages of liver fibrosis and normal live. The in vivo and in vitro models and 3D liver fibrosis organoids, single-cell sequencing, RNA-seq, CHIP-qPCR were used to elucidate the role and mechanism of TrkB in hepatocytes.
Results: In 3D liver organoids and mice with elevated TrkB level in hepatocytes, the liver fibrosis and the inflammatory and fibrotic gene expressions was inhibited. The hepatocytes with TrkB overexpression inhibited the proliferation and activation of HSC. A diminished infiltration of hepatic macrophages in AAV8-TrkB-injected mice and3D liver organoids. The liver biopsy specimens illustrated a negative correlation between TrkB expression and infiltrated macrophage.
The CCR2 receptor signaling pathway in the liver of AAV8-TrkB-injected mice, with a decreased expression of CCL2. Additionally, TrkB suppressed the TGF-β/Smad3 signaling pathway in hepatocyte and the secretion of CCL2.
We identified the overexpression of FOS could significantly increase the level of CCL2 and reverse the inhibitory effect of TrkB on it.
Conclusion: In conclusion, our study delineated that TrkB in hepatocytes attenuates liver fibrosis and the hepatic macrophage recruitment, through the TGF-β/Smad3/c-FOS/CCL2 signaling pathway.
PP-02-247
Bariatric endoscopy improves liver related parameters in MASLD – a systematic review and meta-analysis
Nathanael Chong1, Jian Shiun Tan2, George Boon Bee Goh3,4, Ravishankar Asokkumar3 and Mark CC Cheah3
1Yong Loo Lin School Of Medicine, Singapore;2Department of Internal Medicine, Singapore General Hospital, Singapore;3Department of Gastroenterology and Hepatology, Singapore General Hospital, Singapore;4Duke-NUS Medical School, National University of Singapore, Singapore
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: While bariatric endoscopy (BE) has emerged as a less invasive modality for weight loss, which is also a cornerstone of treatment for MASLD, the benefits of BE in MASLD remains to be clarified. We conducted a systematic review and meta-analysis to explore the potential impact of bariatric endoscopy in the context of MASLD
Methods: MEDLINE, Embase and Web of Science were searched using terms related to MASLD and BE. Studies of MASLD patients who underwent endoscopic sleeve gastrectomy, intra-gastric balloon, duodenal mucosal resurfacing, POSE endosleeve were included. Metabolic and liver related parameters at 6- and 12-months post intervention were compared using a random effects model with data pooling by inverse variance weighting.
Results: We included 14 studies in the meta-analysis with a total cohort size of 436 patients. At 6 months, there was histological improvement measured by the NAFLD activity score (MD = -2.49, 95%CI:[-2.99, -1.97], p = 0.011, I2=0) and no significant worsening of fibrosis stage (MD=0.21, 95%CI:[-1.61, 2.03], p=0.380, I2=0). There were reductions in the controlled attenuation parameter (MD = -46.99dB/m, 95%CI:[-67.29, -26.69], p = 0.003, I2=38.1), ALT (MD = -19.58, 95%CI:[-30.75, -8.42], p = <0.001, I2=65.5) and HbA1c (MD = -0.74%, 95%CI:[-1.20, -0.28], p=0.009, I2=0). Reductions in BMI were observed (MD =-4.08, 95%CI:[-4.95, -3.21] p=<0.001, I2=0) and sustained at 12 months (MD=-6.43, 95%CI:[-6.89, -5.98] p=<0.001, I2=0).
Conclusion: Our study demonstrates that BE is an effective therapy for overweight/obese patients with MASLD, evidenced by improvements in several surrogates of liver and metabolic parameters with durable weight loss.
PP-02-248
Association between triglyceride, il-6 levels in non alcoholic fatty liver disease patients kandou hospital manado
Ari Christian, Jimmy, Bradley Jimmy Waleleng, Luciana Rotty, Fandy Gosal, Andrew Waleleng and Jeanne Winarta
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Faculty of Medicine, Sam Ratulangi University Prof. Dr. R. D. Kandou Hospital, Manado, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Circulating levels of interleukin (IL)-6, a well-known inflammatory cytokine. Elevated IL-6 levels has been reported to be associated with non-alcoholic fatty liver disease (NAFLD) and also triglyceride levels. This study aims to assess the association between triglyceride and IL-6 levels in NAFLD patients.
Material and Methods: A cross-sectional study involving all NAFLD patients in the outpatient ward of the Internal Medicine Department at Kandou Hospital Manado was conducted from January to July 2024. Aged 18–60 years were included in the study. Patients with other cardiovascular infection and Alcoholic were excluded. Subjects meeting the inclusion and exclusion criteria were included in the study until the required sample size was achieved. After data collection, statistical analysis was performed using SPSS version 25.0. Fisher's Exact and Pearson correlation test was used to analyze the association between triglyceride and IL-6 levels. The p-value of ≤0.05 was considered significant.
Results: A total of 48 subjects were included in the study, most were males (52.1%) with mean age of 49.77±11.05 years. Most of the subjects had triglyceride levels of ≤200 mg/dl and IL-6 leels od ≤5 pg/ml. Fisher's Exact test did not show significant association between triglyceride and IL-6 levels (p=0,666). No significant correlation was found between triglyceride and IL-6 levels (p=0,321, r=-0,146). This might be due to several confounding factors which is not adjusted in this study, i.e. comorbidities and history of taking medication for hypertriglyceridaemia.
Conclusion: There is no association between triglyceride and IL-6 levels in NAFLD patients in Kandou Hospital Manado.
PP-02-249
Identification of hereditary PROS1 variation in a patient with non-cirrhotic portal vein thrombosis by WGS
Yingjie Ai, Sitao Ye, Xiaoquan Huang and Shiyao Chen
Zhongshan Hospital, Shanghai, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Genetic factors could account for a subset of patients with portal vein thrombosis (PVT), especially non-cirrhotic patients. Protein S (PS) inactivates factor Va and VIIa and its deficiency is associated with increased risk of venous thromboembolism. PROS1 encodes PS and its variation was often detected in hereditary PS deficiency. Identification of PROS1 variation may explain the occurrence of PVT in non-cirrhotic patients.
Case Description: The patient was a 27-year-old man with PVT and PS deficiency. In September 2022, he was presented to emergency because of acute upper abdominal colic pain. Ultrasound and CT showed thrombosis formation in portal vein, superior mesenteric vein, right distal brachial vein to forearm segment of the cephalic vein, and left forearm segment of the cephalic vein. Autoimmune diseases, blood system disease or malignancy were excluded. WGS revealed a proven pathogenic missense variant of PROS1 (c.1543C>T). The amino acid substitution R515C is presumed to result in unstable PS protein which is degraded intracellularly. Consistent with WGS result, he had a decreased protein S activity (18%). His mother also presented identical PROS1 variation while his father did not. The patient was treated with LMWH and then switched to rivaroxaban. In September 2023, re-examination by ultrasound showed thrombus recanalization.
Discussion: Our study applied WGS as a precise and accurate method to identify the genetic factors underlying non-cirrhotic PVT. Anticoagulation therapy could be recommended for patients with PVT caused by PROS1 mutations.
PP-02-250
A case of Portal Vein Thrombosis in a Patient with Breast Cancer
Yujin Kim
Cheju Halla General Hospital, Jeju-si, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Portal vein thrombosis is an uncommon complication in a patient with breast cancer. Malignancy and several drugs are known to have prothrombic effects. We introduce a case of a patient with history of metastatic breast cancer under treatment with aromates inhibitor, and developed ascites and esophageal varices due to portal vein thrombosis.
Case Description: A 47-year old female patient was referred from the oncology department with six months of abdominal distension. She has been treated for breast cancer for 6 years with surgery, chemotherapy, and radiotherapy.
An aromatase inhibitor, letrozole has been used for years.
Physical examination revealed distended abdomen and shifting dullness.
Initial blood test revealed platetet count 90,000/mL, AST/ALT 34/17 U/L, albumin 3.1 g/dL, and prothrombin time 13.4 sec. Serology test for hepatitis B and C virus infection was negative.
No abnormal result related with thrombophilia was found.
F2 esophageal varices without red color signs were seen on gastroscopy.
Diagnostic paracentesis was performed. The serum-ascites albumin gradient was 2.5g/dL, and ascites protein level was 1.1 g/dL. No malignant cell was found from the ascites.
The patient was diagnosed with pre-hepatic portal hypertension due to portal vein thrombosis associated with breast cancer.
Diuretics including furosemide and spinorolactone was given to control ascites.
Discussion: Malignancy is a hypercoagulable state.
An aromatase inhibitor also can raises the risk for venous thromboembolism.
Development of portal vein thrombosis should be an important differential diagnosis when patients with history of malginancy and under treatment of drugs raise thromboembolic event developed new onset ascites.
PP-02-251
Portal Hypertension Bleeding Caused by Diffuse Liver Metastastic of Pancreatic cancer: a Case Report
Meti Metiani1, Dolvy Girawan2, Muhammad Begawan Bestari3, Nenny Agustanti4 and Eka Surya Nugraha5
1Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;2Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, West Java;3Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;4Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia;5Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Portal hypertension due to diffuse spread of pancreatic cancer to the liver is rare. Massive secondary cancer to the liver results in portal flow resistance, changes in liver architecture, and mechanical compression of tumor infiltration. We report a case of variceal bleeding due to portal hypertension caused by extensive liver metastases in pancreatic cancer.
Case Description: A 55-year-old woman came to the clinic with complaints of black tarry stools for three days. The patient was diagnosed with pancreatic cancer and had a history of gastrointestinal bleeding five years ago. There was no history of chronic liver disease. The liver function was within the normal limit. An enhanced computerised tomography scan of angiography and venography showed the portal vein was partially dilated; there was visible portosystemic collateral formation and multiple massive masses throughout the liver lobes. Endoscopy results showed large fundal varices and grade one esophageal varices.
Discussion: Non-cirrhotic portal hypertension in liver metastases without chemotherapy occurs because of the presence of regenerating nodular hyperplasia, extensive stromal fibrosis, and diffuse tumor infiltration, which causes vascular compression. The prevalence of portal hypertension in liver metastases is unknown. Data on clinical features, management, and prognosis are limited. There are currently no diagnostic criteria, primarily based on imaging techniques. Rapid diagnosis and appropriate monitoring are needed to prevent complications of variceal bleeding or hepatic encephalopathy.
PP-02-252
Acute hemodynamic response to Carvedilol in cirrhotic children with clinically significant portal hypertension
Department of Pediatric Hepatology, Institute of Liver and Biliary sciences, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: To study the proportion of children (2-18year age) with acute hemodynamic response in those with clinically significant portal hypertension (CSPH) and its predictors in cirrhotic children with CSPH.
Material and Methods: Under-18-age cirrhotic children underwent HVPG via the transjugular route. Those with CSPH were administered a single dose of carvedilol (0.2 mg/kg) via oral or nasogastric route. AHR was defined as a decrease in HVPG ≥20% from baseline or absolute HVPG value declining to ≤12 mm Hg, 90 min after carvedilol.
Results: Thirty-eight children (63% males) with median age of 12.7 years (IQR11, 15years) with CSPH were included. Autoimmune liver disease (55%) was the commonest etiology. Median PELD and Child-Pugh scores were 11(IQR 2, 19) and 8(IQR 5, 10).Twenty-one (55%) had prior decompensation– 4 had variceal bleed;18 (47%) had clinically significant varices. The median baseline HVPG was 17 mm Hg (IQR15, 22mm Hg). Following 90 minutes of carvedilol, 18 (47%) patients had an AHR. On multivariate analysis, AHR in HVPG was predicted by portal vein size[Exp(B)=0.492;95% CI=0.289-0.838, P=0.001] and serum sodium [Exp(B)=1.407; 95% CI=1.004-1.971, P=0.033], but not by other demographic, clinical, laboratory, or endoscopic variables. Children who did not achieve AHR had poor SNL at 6 months (OR = 2.308; 95% CI 1.53-3.47, p=0.19) – there were 4 deaths and 2 liver transplants at 6 months of follow-up.
Conclusions: Around half of the children achieved AHR following carvedilol. Larger portal vein diameter and lower serum sodium levels indicative of advanced portal hypertension and liver disease predict non-response to carvedilol.
PP-02-253
Enhancing Furosemide Bioavailability for the Management of Portal Hypertension Using Self Nano Emulsifying Drug Delivery
Pankajkumar Yadav
Shuats, Prayagraj, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: The obsjective of this study was to enhance the bioavailability of furosemide (FURO), an antihypertensive loop diuretic used in the management of portal hypertension, by improving its water solubility, permeability, and absorption after oral administration. To achieve this aim, a novel drug delivery system, Self Nano Emulsifying Drug Delivery System (SNEDDS), was employed.
Materials and methods: Various oils, surfactants, and co-surfactants were tested to determine their ability to improve the solubility of FURO. The self-emulsification region was identified using pseudoternary diagrams, and SNEDDS formulations were developed accordingly. The formulations were characterized using zeta potential determination, droplet size analysis, dilution test, viscosity determination, in vitro dissolution studies, and in vivo pharmacodynamic evaluation.
Results: Mean droplet size of the optimized formulation was found to be 26.8 nm. In vitro performance of the optimized preparation was satisfactory as observed by various analyses such as dilution test, emulsification time, and precipitation assessment. In vitro dissolution studies exhibited that the optimized SNEDDS formulation F3 exhibited a 1.7 fold increase in dissolution efficiency as compared to plain FURO and marketed formulations. In vivo studies showed enhanced bioavailability of F3 in terms of diuretic efficacy.
Conclusion: The study confirms the potential use of SNEDDS formulation as an alternative to traditional oral formulations of FURO to enhance its bioavailability in the management of portal hypertension.
PP-02-254
Correlation of USS confirmed FLD and cirrhosis with VCTE in a cohort of Sri Lankans
Shiran Appuhamy, Suranjan Viraj, Anjalika Madhubhashini, Sakshara Ranasinghe and Chamila Mettananda and Chamila Ranawaka
Colombo North Teaching Hospital, Ragama, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Being a low-middle income country, Vibration-Controlled Transient Elastography (VCTE) (FibroScan) availability is very less in Sri Lanka and most rely on ultrasound scan (USS) for accessing fatty liver disease (FLD) and cirrhosis. Therefore we aim to study the correlation of USS and VCTE for FLD and cirrhosis in Sri Lankans.
Materials and Methods: We conducted a descriptive study from October 2021 to August 2024 (34 months) at Gastroenterology and Hepatology department Colombo North Teaching Hospital Ragama. Patients with USS diagnosed fatty liver disease (FLD) or cirrhosis was referred for VCTE. Non-Significant fibrosis defined by liver stiffness measure (LSM) < 7.5 and significant fibrosis (SF) LSM 7.5 to 19 kPa and cirrhosis LSM > 19 kPa. No Steatosis defined as controlled attenuation parameter (CAP) <238 and steatosis CAP ≥238 dB/m.
Results: Of the 1104 patients identified, 56% were male, mean age was 48. Patients with only FLD on USS was 657 (59.5%) and only cirrhosis was 447 (40.5%). Out of the 657 patients with FLD on USS, VCTE showed steatosis in 575 (87.5%). 82 (12.5%) had no steatosis. Out of 447 cirrhotics on USS, VCTE showed significant fibrosis in 191 (42.7%) and cirrhosis in 182 (40.7%). 74 (16.6%) had no significant fibrosis.
Conclusion: USS showed to be good at identifying steatosis (specificity -87.5%). However USS had a false positive of 59.3% when identifying cirrhosis. VCTE is superior in identifying cirrhosis and quantifying fibrosis over USS. Therefor increasing VCTE availability will make a considerable impact on identifying patients with cirrhosis.
PP-02-255
Rates of Hepatic Fibrosis and Steatosis in a cohort of Sri Lankans referred for VCTE
Colombo North Teaching Hospital, Ragama, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Data on rates of liver Fibrosis and Steatosis among Sri Lankan population is limited. Therefore, we aim to study the rates of hepatic fibrosis and steatosis measured by vibration-controlled transient elastography (VCTE) (FibroScan) in a cohort of Sri Lankans referred VCTE, due to specific indications.
Materials and Methods: We conducted a descriptive study from October 2021 to August 2024 (34 months) at Gastroenterology and Hepatology department Colombo North Teaching Hospital Ragama. Patients referred for VCTE with indications of fatty liver disease(FLD) or cirrhosis on ultrasound scan (USS), obesity (BMI>30kg/m2) or type 2 diabetes were studied. All patients had VCTE. Significant fibrosis (SF) defined by liver stiffness measure (LSM) 7.5 to 19 kPa and Cirrhosis LSM> 19 kPa. Significant steatosis(SS) defined as controlled attenuation parameter (CAP) ≥290 dB/m.
Results: Of the 1990 (100%) patients identified, 56% were male, mean age was 51 years. Indications for VCTE were USS with FLD 657 (33%), USS with cirrhosis 447 (22.5%), obesity 383 (19.2%) and diabeties 503 (25.3%). Rate of SF were 921 (46%) and cirrhosis 361 (18.1%). Rate of SS 1090 (54.8%). Obesity was present among 50.7% patients with SF, and 70.8% with SS. Diabetes was seen in 38.6% patients with SF and 53.9% with SS.
Conclusion: Nearly half of the patients that underwent VCTE had Significant Fibrosis or Significant Steatosis, and 18% had cirrhosis. Obesity and diabetes was prevalent among patients with Significant Fibrosis or Significant Steatosis.
PP-02-256
Incidence and Mortality of Gallbladder Cancer in Hong Kong: a trend analysis
Junjie Huang, Shui Hang Chow, Chenwen Zhong, Sze Chai Chan and Martin Wong
The Chinese University Of Hong Kong, Sha Tin, Hong Kong
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of gallbladder and extrahepatic bile duct cancer in Hong Kong.
Methods: Data on new cases and deaths related to gallbladder and extrahepatic bile duct cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).
Results: In 2020, a total of 445 new cases of gallbladder and extrahepatic bile duct cancer were reported, with an ASR of 2.3. The incidence was slightly higher among males (ASR=2.4) compared to females (ASR=2.1), and remarkably higher in the older population aged over 50 years (ASR=10.7) compared to the younger population (ASR=0.1). There were 151 newly reported deaths, with an ASR of 0.7. The mortality was marginally higher in males (ASR=0.8) than in females (ASR=0.6), and substantially higher in the older population (ASR=3.2) compared to the younger population (ASR=0.07). Trend analysis revealed a significant decreasing mortality trend for both sexes (AAPC: -3.7, 95% CI: -6.5, -0.9, p=0.02) and in the older population (AAPC: -4.3, 95% CI: -7.4, -1.1, p=0.02), while the incidence trends remained relatively stable over the study period.
Conclusion: This population-based study demonstrated a stable incidence trend and a declining mortality trend for gallbladder and extrahepatic bile duct cancer in Hong Kong. Continued efforts on primary prevention through lifestyle modifications could be the next step to further reduce its incidence.
PP-02-257
Factors predicting advanced liver fibrosis in patients with Autoimmune hepatitis-Single Centered experience from Pakistan
Raja Taha Yaseen Khan, Abbas Ali Tasneem and Nasir Hassan Luck
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: To identify factors predicting advanced fibrosis in patients with autoimmune hepatitis (AIH) using non-invasive methods.
Material and Methods: This cross-sectional study was conducted at the Hepatogastroenterology unit of Sindh Institute of Urology and Transplantation, Karachi and included the patients diagnosed with AIH based on the serology and histology. Advanced fibrosis and cirrhosis was defined using the Metavir score on liver biopsy. Multivariate logistic regression analysis was employed to identify predictors of advanced fibrosis.
Results: The study included 162 AIH patients. Advanced fibrosis was observed in 70 (43.2%) patients. Multivariate analysis revealed that female gender (p<0.001) (OR 29.4, 95% CI 4.6-188.6), decreased TLC (p<0.001) (OR 0.2, 95% CI 0.087-0.468) and platelet count (p=0.001) (OR 1.034, 95% CI 1.015-1.054) and increased total bilirubin (p=0.003) (OR 165.8, 95% CI 5.7-485.1), INR (p<0.001) (OR 44.8, 95% CI 14.6-68.1),serum IgG (p=0.025) (OR 0.95, 95% CI 0.91-0.99) and ANA levels of >1:80 (p=0.027) (OR 2.3, 95% CI 1.11-4.66) were significantly associated with the presence of advanced fibrosis and cirrhosis on histology.
Conclusion: This study identified several significant predictors of advanced fibrosis and cirrhosis in patients with autoimmune hepatitis (AIH). Female gender, decreased total leukocyte count (TLC) and platelet count, and raised serum IgG and ANA levels, international normalized ratio (INR) and total bilirubin were all independently associated with advanced fibrosis and cirrhosis. These findings emphasize the importance of these clinical and biochemical markers in identifying AIH patients at risk for advanced liver disease, aiding in early diagnosis and targeted management strategies.
PP-02-258
Clinical Profile of Wilson’s Disease Patients from Tertiary Care Centre of Coastal Karnataka
Abhay Mahajan, Ganesh Bhat and Athish Shetty
Kmc, Manipal, Udupi, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The present study aimed to evaluate the clinical and biochemical characteristics of patients with Wilson's Disease.
Material & methods: This retrospective study was conducted in the department of gastroenterology of tertiary care centre of coastal Karnataka from January 2021to January 2024. Patients with the confirmed diagnosis of Wilson disease irrespective of age and gender were included in the study. Patients’ information including demographic data, clinical and biochemical parameters were reported in a pre-designed format.
Results: A total of 51 patients were included in the study, with a majority being male (30 patients, 58.8%). The mean age of onset was 11.21 ± 1.32 years, the mean delay in diagnosis was 13.12 ± 9.22 months, and the mean age at diagnosis was 15.13 ± 2.42 years. Among the patients, 15 (29.4%) were paediatric patient, and 36 (70.5%) were adults. Kayser-Fleischer rings (51%) were the most common initial presenting feature. Cirrhosis at diagnosis was present in 47.1% patients. On physical examination, 16 patients (45.7%) had hepatomegaly, and 19 (54.3%) reported splenomegaly. Neurological symptoms were present in 22 patients (43.1%), with poor scholastic performance being the most commonly reported neurological issue (13.6%). The majority of patients received zinc treatment (80.4%), followed by penicillamine (19.6%).
Conclusion: This study adds to the existing data on Wilson's disease. In patients with no family history and no tell tale signs of liver cell failure,there might be a delay in diagnosis. Thus an early and guidelines based diagnosis can decrease morbidity and mortality of this treatable disease.
PP-02-259
Triglyceride-Glucose index role in liver fibrosis detection in patients with alcoholic liver disease
1Division of gastrohepatology, Mother Theresa University Hospital Center, Tirana, Albania;2American Hospital Center, Tirana, Albania;3Division of General Surgery, Mother Theresa University Hospital Center, Tirana, Albania
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Alcohol abuse can cause metabolic disorders such as increasing the resistance of insulin and alcoholic liver disease (ALD). The aim of this study is to evaluate the relation of Triglyceride-Glucose (TyG) index, liver fibrosis and Body Mass Index (BMI) in heavy drinking patients.
Material and Methods: This is a retrospective study of 51 patients with alcohol addiction and ALD presented at our University Hospital Center from January-June 2023. Patients with diabetes were excluded from the study. Demographic, laboratory data, and BMI were collected for each patient. AST to Platelet Ratio Index (APRI) score and abdominal ultrasound were used to evaluate the stage of liver fibrosis. Patients were classified as overweight, normal weight and underweight according to World Health Organization.
Results: All the patients were male with a mean age 45.63±10.61 and also heavy drinkers with 22 unit of alcohol consumption per day for 13 years. 49% of the patients had F2-F3 fibrosis, 35 % F3-F4 fibrosis, and 15% had F0-F1 fibrosis. 92% of patients had TyG index over 4.49 suggesting insulin resistance. No association was found between TyG index and stage of liver fibrosis (p=0.934). 65% of the patients were overweight 31% were normal weight and 4 % were underweight. No statistics significant association was found between TyG index and BMI (p=0.862).
Conclusions: Although most of the patients who abuse with alcohol were over the cut off TyG index this was not correlated with their BMI or liver fibrosis staging.
PP-02-260
Fibrosis pattern in patients of MASLD with history of Diabetes mellitus and Dyslipidemia
Anuj Kumar Soni
Government Medical College, Thiruvananthapuram, Kerala, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aim: Metabolic Dysfunction-associated Steatotic Liver Disease(MASLD) has emerged as a major health problem globally. We tried to find the pattern of fibrosis in different groups of MASLD patients with history of (h/o) diabetes mellitus(DM) and dyslipidemia(DLP).
Methods: Cross-sectional observational study of 2240 MASLD patients. Liver fibrosis was assessed using Vibration controlled transient elastography (VCTE). INASL 2022 criteria was applied to define significant fibrosis. One way ANOVA test was applied to compare means in the more than two groups followed by Post Hoc test (Tukey HSD). Chi square test was applied to calculate association of fibrosis with DM and DLP.
Results: 334(14.9%) patients had h/o DM, 460(20.5%) patients had h/o DLP. 177(7.9%) patients had h/o both DM and DLP. Mean VCTE in group of patients having h/o both DM & DLP was 10.33±4.86 kpa and 57.1% patients had significant fibrosis in this group. Mean VCTE was 10.76±5.84 kpa in the DM alone group and 9.01±3.61 kpa in the DLP alone group (p<0.05). 58.0% had significant fibrosis among the patients with h/o DM alone and 45.9% in the DLP alone group (p<0.05).
Conclusion: History of DM is a greater driver of fibrosis than DLP in patients with MASLD. Adding on DLP to DM does not add any significant difference in development of fibrosis in MASLD.
Keywords: MASLD, DM, DLP, VCTE
PP-02-261
Real-World Analysis: The Effect of Recombinant Human Thrombopoietin on Chronic Liver Disease-Thrombocytopenia with Concurrent Infections
Xun Wei and Yi Kang
Henan Provincial People's Hospital, Zhengzhuo, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to assess the therapeutic effect of recombinant human thrombopoietin (rhTPO), on chronic liver disease (CLD) -related thrombocytopenia (TP) with concurrent infections.
Materials and methods: We retrospectively analyzed the clinical data from 259 patients diagnosed with CLD and TP who underwent treatment with rhTPO at the Department of Infectious Diseases, Henan Provincial People's Hospital, from January 2021 to October 2023. And we evaluated changes in platelet count (PLT) and monitored treatment-related adverse events, including bleeding and thrombus formation.
Results: rhTPO significantly increased platelet counts from day 3, effective in both infected and non-infected patients. Patients with Child-Pugh grades A and B demonstrated a more substantial increase in PLT post-treatment compared to those with Child-Pugh grade C. By day 7, significant differences in PLT increments were evident among the three Child-Pugh groups, with respective values of 38.0 × 10^9/L, 32.0 × 10^9/L, and 13.0 × 10^9/L (P = 0.003). The therapeutic impact of rhTPO monotherapy versus its combination with avatrombopag varied, with the former showing a quicker response time compared to the latter (P = 0.011). No serious adverse events were reported.
Conclusion: rhTPO is effective in managing CLD-related TP, reducing bleeding risks, and transfusion reliance, potentially improving patient prognosis.
PP-02-262
Surgical management of Pancreatic Insulinoma: Case report of a rare tumor
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Insulinoma is a rare neuroendocrine tumor that secretes insulin inappropriately causing hypoglycemic episodes. Patients typically present with autonomic and neuroglycopaenic symptoms. Elevated C peptide levels confirms the diagnosis of insulinoma. Preoperative localization of the tumor is important. Here we present a case report of a young lady who was referred from medical clinic with recurrent hypoglycaemic episodes over 2 years. Insulinoma was localized using contrast enhanced computed tomography. Patient was completely free of symptoms after the surgery.
Case Description: We report a case of 25 year old lady who was investigated for recurrent fainting attacks over 2 years. She also had on and off seizures as well. She also has had Faintishnes and dizziness which improves following meals. Her serial random blood sugar levels were between 60 mg/dl -70 mg/dl and there were episodes of below 50mg/dl as well. Contrast enhanced computed tomography was done and it showed features of a insulinoma of the distal pancreas. Laparotomy and excision of insulinoma done and following that the symptoms improved completely. Her post-operative period was uneventful.
Discussion: Insulinoma is a very rare tumor. Clinicians should have a high index of suspicion for this condition in patients with history of recurrent episodes of faintishness or seizures. Proper patient follow-up and repeated analysis helps to recognize early and refer for definitive treatment.
PP-02-263
A serial case of melanoma maligna rectum: should we reconsider abdominoperineal resection for treatment?
Kezia Christy and Alma Wijaya
Padjajaran Univeristy, Bandung, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Melanoma maligna of rectum is an extremely rare disease and poor prognosis with surgical treatment controversy. We present two cases of a 61-y.o- female and 56-y.o- male with melanoma maligna of rectum who underwent APR.
Case reports: A 61-year-old female with a history of rectal bleeding since 2 months ago. Colonoscopy biopsy showed melanoma maligna at distal rectum. CT Scan showed intrahepatic metastasis. The patient underwent APR and died two weeks after APR.
A 56-year-old male with anal pain since 2 months ago. CT Scan showed intrahepatic and pulmonary metastasis. The patient underwent APR and pathological anatomy showed melanoma maligna at rectum. The patient died one month after the operation.
Discussion: The surgical treatment is still controversy. The 5-year survival rate can range from 16 to 34%. In patients who have metastasis at the time of diagnosis, the disease-free survival rate may drop to 16% from 22%. The controversy has been whether APR is needed or wide local excision (WLE) is adequate for complete treatment. APR has a highly morbid operation,but thought to be the best to control anorectal melanoma. WLE are quicker recovery, no need for a stoma, and minimal impact on bowel function. Chemotherapy, radiation therapy, and immune therapy have a limited role.
Conclusion: The rarity of this disease and the limited number of patients who present with early disease, have prevented definitive trials examining the optimal treatment of curable anal melanoma. These 2 cases showed poor result of APR to treat melanoma maligna of rectum.
PP-02-264
Feasibility and outcomes following implementation of modified-ERAS for laparoscopic colorectal cancer surgery in Sri Lanka
1Department of Surgery, National Hospital of Sri Lanka, COLOMBO 4, Sri Lanka;2Department of Surgery, Faculty of Medical Sciences, University of Sri Jayewardenepura, Colombo, Sri Lanka;3Department of Surgery, Colombo South Teaching Hospital, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Enhanced Recovery after Surgery (ERAS) protocols have improved patient outcomes. ERAS with some modifications (mERAS) has been introduced at a resource limited tertiary care centre in Sri Lanka. This study explores the feasibility and potential benefits of mERAS for laparoscopic colorectal procedures in the local context.
Materials and Methods: A prospective study was conducted including diagnosed patients with colorectal carcinoma who underwent laparoscopic surgical management with modified ERAS protocol (n=237, males= 58%, median age: 65 years). A multidisciplinary change in the routine practice was implemented based on mERAS. A descriptive analysis was performed with respect to clinical and treatment characteristics.
Results: Almost all adhered to pre-op mERAS (99%) protocol. Most underwent anterior resection (n=133,56.1%). Most had a pre-operative albumin of >35g/Litre(84%). Some required conversion to open due to technical difficulties (n=27,11.3%). The majority were mobilized on day-1 (65.2%) and day-2 (27.3%) and nasogastric tube removed on day-1(73.3%). Most were discharged from ICU care after post-op day-1 (40%) or day-2 (39%). Abdominal drain was removed by day-4 in 87.8%. Solids were started by day-3 in 85.4%. Post–op complication rates (n=37,15.6%) were within predicted value and most were surgical site infections (n=15) and anastomotic leak was seen in 6 patients. Median hospital stay was 5 days (Range: 4-12).
Conclusion: Modified ERAS was practical and effective in our unit along with complication rates and recovery rates comparable with Western values where the standard ERAS is practiced. Thus, we recommend the modified ERAS protocol for resource limited settings.
PP-02-265
Incidence and pattern of oesophageal cancer in Sri Lanka from 2001-2019
Umesh Jayarajah, Jesuthasan Mithushan and Sanjeewa Seneviratne
Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: An increase in the global incidence of oesophageal cancer has been noted in the last few decades. This study was aimed to describe the trends in the incidence of oesophageal cancer in Sri Lanka which would enable the planning of oesophageal cancer care.
Materials and methods: The trends in the incidence of oesophageal cancer were calculated using the published data from the Sri Lanka Cancer Registry for the period from 2001 to 2019. The trends in the WHO age standardised incidence rates were presented using the Joinpoint regression analysis.
Results: From 2001 to 2019, 26,459 oesophageal cancers were diagnosed, with a male predominance of 53%. The overall mean age was 62.8 years (males=62.2 and females=63.5). The highest incidence was seen in the 70–74 age group. The WHO age-standardised incidence of oesophageal cancer has risen significantly from 5.78 to 8.46 per 100,000 from 2001 to 2019. This increase was prominent till 2016 and thereafter showed a decreasing trend till 2019. The magnitude of the rise in incidence was higher for males [estimated annual percentage change (EAPC):4.6 with a steady trend, p<0.05] compared with females, who had a variable trend with a non-significant EAPC.
Conclusion: A statistically significant increase in the incidence of oesophageal cancer in Sri Lanka was noted from 2001 to 2019, with greater significant proportional increases in men. Further studies, including tumour stage, underlying reasons, and mortality, may help better understand changing patterns of disease burden. Given the rising trend, community-based screening policies should be considered.
PP-02-266
Incidence and pattern of gastric cancer in Sri Lanka from 2001-2019
Umesh Jayarajah, Jesuthasan Mithushan and Sanjeewa Seneviratne
Department of Surgery, Faculty of Medicine, University of Colombo, COLOMBO, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Varying patterns in the incidence of gastric cancer (GC) has been reported globally. This study aims to describe the trends in the incidence of gastric cancer in Sri Lanka.
Materials and methods: The trends in the incidence of gastric cancer were calculated using the published data from the Sri Lanka Cancer Registry for the period from 2001 to 2019. The trends in the WHO age standardised incidence rates were presented using the Joinpoint regression analysis.
Results: From 2001 to 2019, 7914 gastric cancers were diagnosed, with a male preponderance of 71%. The overall mean age was 60.7 years, which was similar for males and females. The incidence of gastric cancer was highest in the 70-74 year age group. The WHO age-standardised incidence of gastric cancer in Sri Lanka has increased from 1.06 to 3.3 per 100,000 from 2001–2016, followed by a decreasing trend to 2.9 per 100,000 in 2019. During the rising trend, the estimated annual percentage change (EAPC) was 8.3 (p<0.05). Females showed a higher proportional increase in incidence (EAPC: 9.9 vs. 8.2).
Conclusion: A rising incidence of gastric cancers is likely due to the combination of better reporting and a true increase in incidence. A greater and more significant proportional increase in incidence was noted in females. Future studies analysing tumour characteristics and mortality would enable a better understanding of the burden of gastric cancer and potential underlying causes for the increasing incidence.
PP-02-267
Chyle Leak: A Dreaded Surgical Complication Following Abdominal Surgeries
Jayamini Kaushalya and Anjana Abayasinghe and Samitha Senevirathne and Duminda Subasinghe
Professorial Surgical Unit, National Hospital-Colombo Sr Lanka, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Chyle leak is a rare but severe complication of abdominal surgeries, leading to high morbidity and mortality due to associated metabolic disturbances, immunosuppression, poor wound healing, and wound-related issues. It is typically suspected during the postoperative period when drain output appears milk-like with elevated volumes. Diagnosis is confirmed biochemically by measuring triglyceride levels in the drainage fluid, which should exceed 100 mg/dL or be higher than serum levels.
Case Report: A 62-year-old female who was investigated for anemia was diagnosed with multiple gastrointestinal stromal tumors (GISTs) originating from the greater curvature of the stomach and fundus, with one large tumor adhering to the pancreas and the transverse colon. After an initially uncomplicated postoperative period, she developed an increase in abdominal drain volume and a change in fluid color to light yellow by postoperative day six. Analysis of the drain fluid revealed elevated triglyceride levels compared to serum and normal amylase levels, confirming a chyle leak. The condition was managed effectively with dietary modifications and pharmacological therapy, avoiding the need for invasive procedures.
Discussion: Prompt diagnosis and conservative management are essential for addressing chyle leaks. Initial treatment typically involves low fat diet, pharmacological options like somatostatin analogues and pancreatic lipase inhibitors, and measures to reduce intra-abdominal pressure, including limiting physical activity and using antitussives and laxatives. Surgical intervention is considered only for refractory leaks or severe metabolic disturbances. This case demonstrates the success of conservative management strategies in resolving a chyle leak without invasive procedures.
PP-02-268
Identifying prognostic factors and independent risk factors in colorectal cancer
1Postgraduate Institute of Medicine, University of Colombo, Sri Lanka;2Health Data Science Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka;3Department of Pathology, Faculty of Medicine, University of Kelaniya, Sri Lanka;4Department of Surgery, Faculty of Medicine, University of Kelaniya, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Establishing factors influencing the overall survival is crucial for improving prognostication and treatment in colorectal cancer(CRC). This study aims to identify key prognostic factors and independent risk factors in CRC by analyzing survival outcomes.
Materials and Methods: In CRC patients undergoing surgical resection from January-2013 to May-2019, followed until May-2024, Age, Sex, tumor differentiation(TD), T stage, N stage, having a positive LN (PLN) & LVI along with time since surgery to last follow-up/death were collected. Survival rates were estimated using Kaplan-Meier curves and the Log-rank test(LRT) the level of significance being p<0.05. Cox proportional-hazard models identified survival-associated features, with forward variable selection determined by the log-likelihood ratio test.
Results: Of 137 participants, 54(39.42%) were males, with a median age of 61(IQR: 52-68). 34 out of 58 deaths were caused by CRC. The overall 5-year-survival(OS) rate was 60.4%(95%CI, 52.5%-69.5%), and the corresponding disease-specific survival(DSS) rate was 72.2%(95%CI, 64.5%-80.8%). When considering OS, the following factors indicated a significant difference in the LRT: LVI (P < 0.0001), N0-N1-N2 stage (P=0.022, P=0.051), PLN (P = 0.0026, TD(P=0.0003), T4 Stage(P=0.047).
Results of forward selection showed the model with LVI, age, and N stage was the best to explain OS. The hazard ratios were: LVI =3.094(95% CI,1.782-5.372, P < 0.0001), age =1.029(95% CI,1.005-1.054, P=0.0173), and N stage =1.545(95% CI,1.062-2.247, P=0.0229).
Conclusion: For survival, age, tumor differentiation, T stage, N stage, and LVI were significant factors. LVI, age, and N stage were independent prognostic factors, with LVI being the strongest predictor.
PP-02-269
Prognostic significance of categorizing lymphovascular invasion as nodal stage n1 in colorectal cancer
1Postgraduate Institute of Medicine, University of Colombo, Sri Lanka;2Health Data Science Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka;3Department of Pathology, Faculty of Medicine, University of Kelaniya, Sri Lanka;4Department of Surgery, Faculty of Medicine, University of Kelaniya, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Positive nodal metastases indicate lymphovascular invasion(LVI) in colorectal carcinoma(CRC) is a known concept. The reverse situation has not been investigated. This study aims to analyze the prognostic significance of categorizing LVI-positive nodal negative cases as stage N1.
Materials and Methods: The nodal stage, LVI status, and time since surgery to the last follow-up/death were obtained from the database for patients who underwent surgical resection from January-2013 to May-2019, with follow-up until May-2024. Cox proportional-hazard models were used to identify features associated with survival. LVI-positive, N0 cases were transferred to N1 stage, establishing a new model(NM) for statistical analysis for comparison with the existing model prior to transfer -Traditional model(TM).
Results: Of 137 participants, 41(29.93%) were LVI positive. Nodal stages were N0-65, N1-51, N2-21. Of the 96 LVI negative cases, 53 were in N0 stage and 43 were nodal positive. Among 41 LVI positive cases, 29had nodal metastasis, while 12 did not. These 12 cases were transferred to N1. Hazard ratio for overall survival (HR) for LVI in NM was 3.094(95%CI,1.782-5.372,P<0.0001) compared to 2.706(95%CI,1.537-4.764,P=0.0006) in TM. HRs for TM and the NM were 1.545(95%CI,1.062-2.247,P=0.0229) and 1.760(95%CI,1.169-2.648,P=0.0068), respectively.
Transferring LVI positives changed the N stage coefficient P-value from 0.0229 to 0.006753 (Wald test) and increased concordance from 0.725 to 0.726 in NM.
Conclusion: The NM demonstrate superior model evaluation parameters than TM. Transferring LVI positives from N0 to N1 improved prognosis accuracy, suggesting need to explore stage migration from N0 to N1 with positive-LVI without nodal metastases in a larger cohort.
PP-02-270
Clinicopathological Characteristics and Lymph Node Metastasis Rates in Early Gastric Lymphoepithelioma-like Carcinoma
Tae-Se Kim, Youngeun Oh, Ji Yeong An, Yang Won Min, Hyuk Lee, Byung-Hoon Min, Jun Haeng Lee, Poong-Lyul Rhee and Jae J. Kim
Samsung Medical Center, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Lymphoepithelioma-like carcinoma (LELC) is a rare subtype of gastric cancer. We aimed to identify the clinicopathological features and rate of lymph node metastases (LNM) to investigate the feasibility of endoscopic submucosal dissection (ESD) for early gastric LELC confined to the mucosa or submucosa.
Material and Methods: We compared the clinicopathological characteristics of 116 early gastric LELC patients and 5,753 early gastric well- or moderately differentiated (WD or MD) tubular adenocarcinoma patients treated by gastrectomy.
Results: Compared to WD or MD early gastric cancer (EGC) patients, early LELC patients were younger and had a higher prevalence of proximally located tumors. Despite more frequent deep submucosal invasion (86.2% versus 29.8%), lymphatic invasion was less frequent (6.0% versus 16.2%) in early LELC patients than in WD or MD EGC patients. Among tumors with deep submucosal invasion, tumor size was smaller, lymphatic invasion was less frequent (6.0% versus 40.2%) and the rate of LNM was lower (10.0% versus 19.4%) in patients with LELC than those with WD or MD EGC. Overall rate of LNM in early LELC patients was 8.6% (10/116). Risk of LNM in patients with mucosal, shallow submucosal invasive, or deep submucosal invasive LELC was 0% (0/6), 0% (0/10), and 10% (10/100), respectively.
Conclusion: Early LELC is a distinct subtype of EGC with more frequent deep submucosal invasion but less lymphatic invasion and LNM than WD or MD EGCs. ESD may be considered curative for patients with early LELC confined to mucosa or shallow submucosa, given its negligible rate of LNM.
PP-02-271
Aberrant right hepatic artery (ARHA) with dominant supply during pancreaticoduodenectomy: We report you decide
Duminda Subasinghe1,2, Nimeshana Bandara2 and Sivasuriya Sivaganesh1,2
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Aberrant right hepatic arteries (ARHA) occur in 15-20% of the population. Most arise from the superior mesenteric artery (SMA) and pass posterior to the head of the pancreas (HoP) and less commonly through or anterior to the HoP. Aberrant RHAs may be accessory or replaced (rARHA).
Case description: A 61-year-old male with a bleeding duodenal malignancy prepared for a pancreaticoduodenectomy had a replaced RHA passing posterior to the HoP on CT. This was confirmed at surgery with early isolation and preservation. Postoperative recovery was uneventful, and histopathology confirmed an R0 resection. The key challenges when operating with ARHAs are preservation of arterial flow to the right lobe of the liver and biliary tract and obtaining R0 resection. Meticulous preoperative CT imaging and evaluation of the vasculature by the surgeon himself, as well understanding of surgical anatomy and surgical planning are crucial to achieving the above outcomes.
Discussion: Identification and preservation of an rARHA is mandatory during pancreatico-biliary resections. Failure could lead to ischaemia of the right hepatic lobe and biliary tree with early bilio-enteric anastomotic breakdown or late strictures. Pre-operative evaluation of arterial anatomy and planning surgical strategy prevents inadvertent injury. A ventral instead of conventional dorsal approach, early right to left retro-pancreatic exposure of the SMA are advocated. There are 4 options to manage ARHA. Preservation, Transection and reconstruction, Sacrifice (presence of s dominant RHA) and Pre-operative embolization to facilitate collateral formation.
PP-02-272
Forgotten remedy for duodenal tumours: Surgical ampullectomy in the era of interventional endoscopy
Duminda Subasinghe1,2, Nimeshana Bandara2, Nilesh Fernandopulle1,2 and Sivasuriya Sivaganesh1,2
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Ampullary or duodenal adenomas are uncommon digestive tract tumours. Conventional treatment with surgical ampullectomy and pancreaticoduodenectomy has been largely superseded by endoscopic resection. However, large, broad-based tumours still require surgical excision.
Case description: Two females aged 28 (patient 1) and 61 years (patient 2) investigated for dyspeptic symptoms had large polyps in the 2nd to 3rd parts of the duodenum. Endoscopic resection was not done due to their size and broad-based attachment. P1 had an open transduodenal ampullectomy and P2 a local papillectomy. Access was via right subcostal and midline laparotomies followed by Kocherisation and a longitudinal duodenotomy. Submucosal saline injection was performed to facilitate mucosal excision.
P1 had a tumour arising from the ampulla of Vater enclosing the common pancreatico-biliary channel. She underwent an ampullectomy and a papilloplasty. P2 had an anterior wall polyp of the 2nd and 3rd part of the duodenum and underwent a polypectomy A full thickness defect resulting from excision was primarily closed. The duodenotomies were closed in 2 layers. Both had uneventful post-operative periods. Both patients had tubular adenoma with low grade dysplasia.
Discussion: Open transduodenal excision is a safe and effective therapeutic option for large, benign duodenal polyps not amenable to endoscopic resection. Caution should be exercised for lesions arising in the pancreatico-mesenteric aspect to avoid challenges with closure.
PP-02-273
Atypical Hepatic Metastasis of Pancreatic Adenocarcinoma Unveiled Through Persistent and Unresolved Pyrexia
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka;3Department of Pathology, University of Colombo, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The diagnosis of neoplastic fever poses a considerable diagnostic challenge due to the necessity of excluding identifiable etiologies. Fever as a predominant clinical manifestation of pancreatic adenocarcinoma is an uncommon occurrence, with limited instances documented in the medical literature. Herein, we present an atypical case of metastatic pancreatic carcinoma primarily characterized by pyrexia of unknown origin (PUO).
Case Description: A 63-year-old male of Sri Lankan origin, a non-smoker with a medical history notable for diabetes, hypertension, and dyslipidemia, presented with a two-month history of fever, anorexia, and weight loss. Despite completing treatment for positive serology for paratyphi, his symptoms persisted, and inflammatory markers remained elevated, while all other infectious screening tests returned negative results. Subsequent evaluation revealed hypodense lesions in the distal pancreas and multiple liver lesions with ring enhancement on imaging studies. Histopathological examination obtained via CT-guided biopsy confirmed the diagnosis of pancreatic adenocarcinoma with liver metastasis. Despite receiving symptomatic management for fever and palliative care, the patient ultimately succumbed to the disease.
Discussion: In cases where atypical liver metastases are observed with evidence of ring enhancement on computed tomography (CT) imaging, biopsy becomes imperative for accurate diagnosis and subsequent treatment planning. Hence, in the setting of atypical metastatic pancreatic adenocarcinoma, the recommended approach entails palliative chemotherapy alongside pharmacological management of fever symptoms.
PP-02-274
Association between intestinal fibrosis and clinical recurrence after ileocolonic resection in stenotic Crohn's disease patients
Tianyi Che, Jiaxin Wang, Yao Zhang, Yubei Gu and Duowu Zou
Department of Gastroenterology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: We aim to investigate the association between fibrosis at the site of intestinal stenosis and clinical recurrence after ileocolonic resection in patients with stenotic Crohn's disease.
Materials and Methods: A retrospective, single-center study was conducted on stenotic Crohn's disease patients who received ileocolonic resection between January 2020 and December 2022. The most representative pathological slides from the resected stenotic intestinal tissues were selected. We used Masson's trichrome staining to visualize collagen fibers and calculated the proportion of collagen fiber area in the slides using the Image-Pro-Plus software to assess the degree of intestinal fibrosis. Baseline information and postoperative clinical recurrence were collected through electronic medical record systems and telephone follow-up. Survival analysis and Cox regression models were used to assess the impact of intestinal fibrosis on clinical recurrence.
Results: Sixty-nine patients were included in this study. 29 (42%) of the patients experienced clinical recurrence during the follow-up period. Medium (HR 16.545, 95%CI 2.164-126.464, P=0.007) and high (HR 32.997, 95%CI 4.304-252.986, P=0.001) degree of intestinal fibrosis were associated with clinical recurrence after surgery in patients with stenotic Crohn's disease. This association remained significant when considering fibrosis only in the mucosa, submucosa, or muscular layers.
Conclusion: The degree of fibrosis at the site of intestinal stenosis was significantly associated with clinical recurrence after ileocolonic resection in patients with stenotic Crohn's disease. Intestinal fibrosis should be considered in postoperative treatment and follow-up decision-making.
PP-02-275
Recurrence after ileo-caecal resection in Crohn’s disease – Lower with post-operative colonoscopy within 12 months
Millicent DeVries and Rajan Patel
Christchurch Hospital, Christchurch, New Zealand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Ileo-caecal (IC) resection for patients with small bowel Crohn’s disease is common. Complications of disease recurrence at the anastomosis can be prevented with optimised post-operative management. We aimed to investigate the incidence of disease recurrence following IC resection and identify associated factors.
Materials and Methods: Retrospective review of the most recent 50 patients who underwent IC resection for Crohn’s disease with at least 12 months follow up. Data obtained from Business Intelligence Data & Analytics from 2018-2023. Electronic records reviewed for demographics, post-operative colonoscopy findings and medications initiated post-surgery. Statistical analysis performed using Fishers Exact test.
Results: Cohort comprised 50 patients with median age 39 years (range 16-76); 42% female. Mean follow-up duration from surgery 1268 days (range 401 - 2085). Seventy-four percent of resections were laparoscopic, and 88% received prophylactic metronidazole. Post operative thiopurine or biologic initiated in 44% of patients (18% received thiopurine only, 82% biologic +/- thiopurine). Post-operative colonoscopy performed in 62% of patients, with mean time to endoscopy 19 months (range 6-55 months); 22% had colonoscopy within 12 months.
Among 44 patients assessed endoscopically or radiologically, 45.6% showed anastomotic recurrence. Recurrence did not differ based on post-operative metronidazole use (p=0.5). However, patients who underwent colonoscopy within 12 months of surgery had a significantly lower recurrence rate (p=0.04). No repeat resections occurred during the follow-up period.
Conclusion: Post-operative metronidazole is not associated with reduced recurrence rates in the biologic era. Post-operative colonoscopy, and not radiological assessment, within the first year of surgery correlates with disease remission.
PP-02-276
Clinico-pathological characteristics of patients with malignant liver lesions: preliminary results from an HPB surgical unit
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2Department of Anaesthesiology and Critical care, University of Colombo, Colombo, Sri Lanka;3University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Liver malignancies are the fourth commonest cause of cancer-related deaths globally. Of these, secondary liver malignancies are more common, especially colorectal metastases. This study studies the demography, clinicopathological features and management of malignant liver lesions (MLL) in a tertiary hepatobiliary center.
Materials and Methods: A prospective study was conducted on patients with MLL (n=44) presenting from May 2023 – May 2024. Data on clinicopathological and radiological features were obtained from a custom-made database, followed by a descriptive analysis.
Results: The majority were males (84%) with a mean age of 63.9 (38-82) years. Most patients (47.7%) were from the Colombo district. Constitutional symptoms were common (59%). Alcohol (n=25,56.8%) and diabetes (n=24,54.5%) were major associations, with (n=11,25%) diagnosed with chronic liver disease. Most lesions on CT were multifocal (n= 23, 52.2%), followed by solitary lesions (n= 17,38.6%). Primary malignancies included HCCs (n=19,43.1%), intrahepatic-cholangiocarcinomas (n=3,6.8%) and combined HCC/Cholangiocarcinoma (n=2,4.54%). Metastases were mainly from the pancreas (n=6,13.6%), large bowel (n=5,11.3%) and distal CBD (n=4,4.54%). Management included palliation (n= 20,45.4%), hepatectomy (n=13,29.5%), TACE (n=6,13.6%), and ablative therapy (n= 3, 6.8%). Surgery included right hepatectomy (n=4, 30.7%), left lateral sectionectomy (n=4,30.7%), non-anatomical resection (n=2,15.3%), segmentectomy (n=2,15.3%) and parenchymal sparing hepatectomy (n=1,7.69%).
Conclusion: The clinical and demographic spectrum of malignant liver tumors observed in this cohort are similar to that in Asia. However, liver secondaries in Asia predominantly originates from lung and colorectal primaries compared to pancreatic and colorectal origins in our cohort, which affects survival rates.
PP-02-277
Successful management for necrotizing fasciitis due to colon cancer with intracorporeal jejunocolic bypass
Rio Miyashita and Tadayoshi Yamaura and Yuki Tokuyama and Masasaaki Moriyama and Makoto Kawase and Satoshi Kanto and Yosuke Kinjo and Nobukazu Kuroda
National Hospital organization Himeji Medical Center, 68, Honmachi, Himeji, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Necrotizing fasciitis (NF) is a severe infectious disease. NF derived from colon cancer perforation is rare. The treatment for this type of NF needs balancing cancer and infection treatment. Nevertheless, keeping this balance is difficult, especially in the elderly.
Case report: The patient is a 90–year–old female with dementia. The abdominal wall bulge had appeared from 2 weeks ago. Abdominal CT scan and colonoscopy revealed ascending colon cancer that invaded the abdominal wall and right external iliac artery. Additionally, colon perforation causes NF mainly in right side abdominal wall, spreading beyond the midline on ventral side, to the left side on dorsal side. As initial therapy, she was treated with antibiotic administration, and not extensive surgical debridement because of her age. After 4 weeks of this, her condition became better. However, she needed the prevention of colonic juice to flow into an abdominal wall for oral intake and infection control. Extended resection of cancer was hard for her. Stoma management was also hard due to her dementia. Finally, we performed laparoscopic intracorporeal jejunocolic bypass with all trocars placed the left side of abdominal wall: normal healthy side. On 24 postoperative days, she was discharged without any complications including surgical site infection.
Discussion: The strategy for NF due to unresectable cancer has no absolute answer. We believe that less invasive treatment for this severe and poor prognosis disease improves their quality of life. We present successful management for NF owing to colon cancer in an extremely old patient.
PP-02-278
Caterpillar hump of right hepatic artery at laparoscopic cholecystectomy: Picture Story based on case report
Duminda Subasinghe1,2, Nimeshana Bandara2 and Sivasuriya Sivaganesh1,2
1Division of HPB/GI Surgery, Department Of Surgery, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, The National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Anomalies in and around the hepatocystic triangle are common (20-50%) with variations in the origin, course and branches of the RHA documented. A sinuous, tortuous RHA coursing through the hepatocystic triangle is a less common but potentially dangerous anomaly. This morphology of the RHA is referred to as the caterpillar or Moynihan’s hump.
Case Description: A 26-year-old female presenting with biliary pain and elevated bilirubin levels was found to have choledocholithiasis. She underwent endoscopic retrograde cholangiopancreatography (ERCP) and ductal clearance followed by a laparoscopic cholecystectomy 6 weeks later. During the dissection of the Calot’s triangle, a prominent tortuous artery was observed passing anterior to a short cystic duct. This was identified as a single loop caterpillar or Moynihan’s hump of the right hepatic artery (RHA) from which a short cystic artery originated (Figure 1,2). Cholecystectomy was completed after the demonstration of the critical view of safety and division of the cystic artery and duct between clips.
Discussion: The incidence of the RHA caterpillar hump is 7%, with 40% passing anterior and 60% passing posterior to the cystic duct. Single and double loop configurations account for 55% and 45% respectively. A short cystic artery arising from this hump may be avulsed or the RHA may be accidentally divided during dissection in the Calot’s triangle.
Demonstrating the critical view of safety will minimize bilio-vascular injuries at laparoscopic cholecystectomy. Awareness and anticipation of vascular anomalies in the hepatocystic triangle and careful dissection will minimize inadvertent injuries.
PP-02-279
The safety of hepatobiliary-pancreas surgery in elderly patients : retrospective analysis using comprehensive frailty assessment
Mirang Lee
Asan Medical Center/Division of Hepatobiliary and Pancreatic Surgery, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: In the field of surgery, frailty is an independent risk factor for mortality, morbidity, length of stay, and postoperative complication. In an aging society, as the age of patients undergoing hepatobiliary-pancreas surgery increases and the incidence increases, we aim to investigate the impact of frailty on the occurrence of postoperative complications.
Materials and Methods: From June 2021 to April 2024, we retrospectively analyzed 227 patients who underwent comprehensive frailty assessment among patients who underwent hepatobiliary-pancreas surgery at Asan Medical Center. We measured a 34-item frailty index constructed using Rockwood’s deficit accumulation approach. Demographics, clinicopathologic, and surgical data were retrieved for analysis.
Results: Out of 227 patients, there were 25 frail patients and 202 non-frail patients. In the frail group, 36.0% and in the non-frail group, 5.9% received preoperative interventions including nutritional support, drug adjustment, and exercise education through preoperative geriatric consultation. There were no differences between frail and non-frail patients in complications (32.0% vs. 36.1%, p=0.684), ICU admission (20.0% vs. 15.3%, p=0.548), postoperative stay (12.6 vs. 13.7 days, p=0.576), readmission (9.1% vs. 7.6%, p=0.806).
Conclusions: While frailty is an important factor that increases the burden after surgery, if appropriate preoperative evaluation and subsequent perioperative intervention are conducted, it should not be the absolute contraindication for hepatobiliary-pancreas surgery.
PP-02-280
Impact of Roux-en-Y Gastric Bypass on Esophageal Motility in Morbidly Obese Patients
1Faculty Of Medicine Chulalongkorn University, Bangkok, Thailand;2Department of Physiology, Faculty of Medicine, Chulalongkorn University, Thailand;3Center of Excellence in Neurogastroenterology and Motility, Faculty of Medicine, Chulalongkorn University, Thailand;4Department of Surgery, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Thailand;5Treatment of Obesity and Metabolic Disease Research Unit, Faculty of Medicine, Chulalongkorn University, Thailand;6Division of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Thailand
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Obesity increases the risk of gastroesophageal reflux disease (GERD). Roux-en-Y gastric bypass (RYGBP) effectively treats GERD in morbid obesity. This study examined esophageal motility changes before and after RYGBP.
Materials and Methods: Morbidly obese patients (BMI ≥40 kg/m² or ≥35 kg/m² with comorbidities) planned for RYGBP were prospectively enrolled. Gastrointestinal symptoms and high-resolution esophageal manometry (HRM) were evaluated before and after surgery. Ten swallows of a standardized 5 mL liquid were performed in supine and upright positions. The Manoview software version 3.0 (Medtronic Inc., MN, USA) analyzed parameters: lower esophageal sphincter (LES), integrated relaxation pressure (IRP), distal latency (DL), and distal contractile integral (DCI).
Results: Nine patients (age 42±11 years, BMI 44.53±4.7 kg/m²) were enrolled. Preoperatively, 40% experienced nausea and heartburn. Post-surgery, heartburn resolved. HRM was conducted with a median follow-up of 42±25 days. Patients had a significant BMI decrease (36.8±5.7 kg/m², p=0.028). Pre-surgery manometry showed normal findings with median supine IRP slightly elevated (13.35 mmHg) but normal in the upright position. Post-surgery, all manometric parameters tended to decrease without statistical significance. Preoperative LES pressure was 32.50±6.3 mmHg (upright) and 43.50±21.8 mmHg (supine); IRP was 8.73±2.6 mmHg (upright) and 13.35±6.3 mmHg (supine). Postoperative LES pressure was 24.95±6.3 mmHg (upright) and 33.35±16.1 mmHg (supine); IRP was 2.85±3.7 mmHg (upright) and 7.85±10.8 mmHg (supine). No significant change in normal peristalsis (p<0.05).
Conclusion: There were no changes in esophageal motility post-RYGBP in morbidly obese patients. GERD symptom improvement may result from other mechanisms such as decreased intragastric pressure or accelerated gastric emptying.
PP-02-281
Gut Microbiota as Potential Biomarker for Metabolic dysfunction-associated fatty liver disease (MAFLD): A Systematic Review
Hery Djagat Purnomo1, Cecilia Oktaria Permatadewi1, Kevin Tandarto2, Hesti Triwahyu Hutami1, Didik Indiarso1, Agung Prasetyo1 and Hirlan Hirlan1
1Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Semarang, Indonesia;2Department of Internal Medicine, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Metabolic dysfunction-associated fatty liver disease (MAFLD) is the new global epidemic. Previous studies revealed the relationship between dysbiosis and MAFLD's progression via immune dysregulation and metabolic shifts. This review seeks to establish the role of gut microbiota as a biomarker for fibrosis in MAFLD.
Method: A comprehensive search will be performed across multiple electronic databases including PubMed, Scopus, and Web of Science. The search strategy will combine relevant Medical Subject Headings (MeSH) terms and keywords related to "gut microbiota", "MASLD", “NAFLD”, “MAFLD”, "fatty liver disease", “Steatohepatitis”, and "biomarker".
Results: A total of 16 studies were included in this review. Recent studies have extensively explored the gut microbiota's composition across various stages of MAFLD. Studies highlighted that Proteobacteria, Actinobacteria, and Verrucomicrobia were positively correlated with liver steatosis, whereas Firmicutes and Euryarchaeota showed negative correlations. Another investigation using 16S rRNA sequencing noted a decrease in Clostridium sensu stricto abundance with the onset of steatosis and fibrosis, with Escherichia/Shigella potentially serving as a discriminating microorganism for fibrosis. In Asia, a study in China using shotgun metagenomic sequencing found reduced Methanobrevibacter and Phascolarctobacterium levels, and increased Slackia and Dorea formicigenerans in MAFLD. Recent study from South Korea revealed that ET-P dominated by the genus Prevotella, constitutes a high-risk group for MAFLD in the Asian population. Japanese research noted the decreased Ruminococcaceae and Faecalibacterium, suggesting these changes as biomarkers for disease severity in Asian populations.
Conclusion: Increasing evidence from clinical research underscores the significant involvement of gut microbiota and their metabolites in MAFLD progression
PP-02-284
Therapeutic Potential of Probiotics in Radiation-Induced Gastrointestinal Toxicity in Cervical and Rectal Cancer: A Meta-Analysis
Ninda Septia Yuspar, Eddy Yuristo and Suyata Suyata
Sriwijaya University, Palembang, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Radiation-induced gastrointestinal (GI) toxicity is a significant adverse effect in patients undergoing radiotherapy for cervical and rectal cancer, often leading to severe diarrhoea and compromising treatment outcomes. Probiotics have been proposed as a therapeutic option to mitigate these toxicities.
Objectives: To systematically review and quantitatively synthesize the evidence on the therapeutic potential of probiotics in reducing radiation-induced GI toxicity, specifically focusing on the incidence of diarrhoea and severe diarrhea in patients with cervical and rectal cancer.
Methods: A comprehensive literature search was conducted in PubMed, Embase, and Cochrane Library databases to identify studies assessing the impact of probiotics on radiation-induced GI toxicity. Inclusion criteria were studies reporting on the incidence of diarrhoea and severe diarrhoea, defined by the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) as grade ≥3. Data were extracted and pooled using a random-effects model, and odds ratios (OR) were calculated. Heterogeneity was assessed using the I² statistic.
Results: A total of 7 studies, including 1103 patients, met the inclusion criteria. The pooled analysis showed that probiotics insignificantly reduced the incidence of diarrhoea (OR 0.71, 95% confidence interval [CI] 0.38-1.33) and had a significant effect on reducing severe diarrhoea (OR 0.22, 95% CI 0.07-0.69). Heterogeneity among studies was moderate (I² = Z%).
Conclusion: This meta-analysis indicates that probiotics have potential therapeutic benefits in reducing radiation-induced GI toxicity in patients with cervical and rectal cancer, particularly in decreasing the incidence of severe diarrhea.
Compassion fatigue, burnout and professional quality of life among organ transplant coordinators: A descriptive study
Aye Su Mon
National University Centre for Organ Transplantation, Singapore
Objectives: To access compassion fatigue, burnout and professional quality of life among liver and kidney transplant coordinators.
Materials and Methods: A non-experimental, exploratory descriptive study was carried out by using Professional Quality of Life Scale Version 5 (ProQOL-V) among liver and kidney transplant coordinators in one of the transplant centers in Singapore. A total of 11 transplant coordinators participated in this study.
Results: Based on the responses, there is moderate level of compassion fatigue, burnout is present among the transplant coordinators. But, on the other hand, there is an average level of compassion satisfaction for being able to help patients as transplant coordinators.
Conclusion: Healthy and supportive work environment play an essential role in the mental well-being of healthcare professionals which in turn contributes to overall patients’ care quality, enhanced job satisfaction and organizational success. Studies with a greater number of participants will be required for better understanding and for implementation of necessary changes.
PP-02-314
Numb mitigates radiation-induced intestinal epithelial cell senescence via a Polo-like kinase 1-dependent pathway
Yongtao Yang1, Xiao Hu1, Yuwei Pan1 and Tao Liu2
1Department of Gastroenterology, Chongqing University Cancer Hospital, Chongqing, China;2Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to explore the impact of Numb on senescence of intestinal epithelial cells and injury to the intestinal mucosal barrier by modulating Plk1 activity following radiation.
Materials and Methods: C57BL/6J mice and colonic cells were exposed to radiation to simulate radiation-induced intestinal injury in vivo and in vitro. Intraperitoneal injection of Numb antisense oligodeoxynucleotides was used to downregulate Numb expression in mouse intestinal epithelial cells. Intestinal tissue pathology was evaluated through histological staining. Intestinal mucosal barrier permeability was evaluated through FITC-dextran assay and serum DAO concentration. Gene and protein expression levels were assessed using quantitative RT-PCR, immunoblotting and immunohistochemistry, while flow cytometry was employed to analyze apoptosis rates and cell cycle progression.
Results: Inhibiting Numb expression in intestinal epithelial cells exacerbated histopathological damage to the intestinal mucosa post-radiation, leading to increased intestinal mucosal barrier permeability. This inhibition also resulted in elevated levels of γH2AX, p21, and SA-β-Gal in intestinal epithelial cells, along with enhanced expression of inflammatory factors. In vitro experiments revealed that interfering with Numb expression led to an accumulation of colon cells in the G2 phase, promoting cell senescence and upregulating inflammatory factors. Additionally, interfering with PLK1 expression was found to enhance radiation-induced intestinal cell senescence, while overexpressing PLK1 could reverse this senescence induced by Numb knockdown.
Conclusion: This study highlights the crucial role of Numb in safeguarding intestinal mucosal barrier function by inhibiting radiation-induced senescence of intestinal epithelial cells. Modulating the Numb-PLK1 signaling pathway may present a promising therapeutic target for radiation enteritis.
PP-02-315
Clinicopathological characteristics, immunohistochemical findings, and genetic mutations in carcinoma of the pancreatic neck
Yoshihiro Hamada and Yusuke Ishida and Masatoshi Kajiwara
Fukuoka University Hospital, Fukuoka, Japan
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: The pancreatic neck (Pn) is an important region because it is located next to important blood vessels like the portal vein, superior mesenteric vein, common hepatic artery, and gastroduodenal artery, and it forms the resection line in pancreatoduodenectomy or distal pancreatectomy for pancreatic neoplasms. We analyzed the clinicopathological characteristics, immunohistochemical findings, and genetic mutations of IDCPn.
Methods: Among 487 patients diagnosed with IDCP, 4 (5.2%) had tumors located in the Pn with diameter <2 cm.
Results: The IDCPn patients comprised two men and two women with an age range of 67–75 years. Two patients underwent pancreatoduodenectomy, and two patients underwent total pancreatectomy. All four tumors were moderately differentiated adenocarcinoma. The four tumors had obvious carcinoma in situ (CIS) infiltration (24–56 mm). One patient had regional lymph node metastasis. The tumor cells showed p53 abnormalities were recognized in all patients, but neither p16 inactivation nor DPC4 inactivation was observed. KRAS mutations on codons 12 and 61 were not detected in the patients. Despite a lack of intraductal papillary mucinous neoplasm (IPMN) findings on preoperative imaging or pathological examinations, GNAS mutations at codon 201 were detected in two patients. One patient died of liver metastasis, two patients remained alive without recurrence, and one patient died of another cause.
Conclusion: Surgical resection of IDCPn should be conducted carefully because IDCPn can have extended CIS. IDCPn showed typical morphological findings of IDCP, but its carcinogenesis and progression may differ from IDCP at other anatomical sites based on the genetic mutation analysis.
PP-02-316
Morphological severity in the chronic pancreatitis could guess the severity of the pancreatic exocrine insufficiency
Min Kyu Jung1 and Byung Ik Jang2
1Kyungpook National University School Medicine, Daegu, South Korea;2Yeungnam University College of Medicine, Daegu, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: The association between pancreatic exocrine insufficiency (PEI) and morphologic findings in chronic pancreatitis has not been fully studied yet. Thus, the aim of this study was to investigate the correlation between PEI severity and computed tomography (CT)-based morphological severity in patients with chronic pancreatitis.
Methods: This multicenter retrospective study included 180 patients with chronic pancreatitis aged 18 years or older between January 2018 and December 2021. PEI severity was measured by PEI questionnaire (PEI-Q). Morphological severity was measured using a CT-based scoring system including pancreatic duct caliber, pancreatic duct stricture or intraductal obstructing calculus, pancreatic atrophy, and pancreatic calcification. In addition, 35 patients who received pancreatic enzyme replacement therapy (PERT) were evaluated by PEI-Q to determine whether PEI improved after PERT.
Results: PEI severity was normal (n = 89), mild (n = 69), moderate (n = 14), or severe (n = 8). Severities of pancreatic duct caliber and pancreatic duct stricture or intraductal obstructing calculus had small but significant associations with PEI severity (Cramer’s V = 0.121 and 0.141, respectively). Severities of pancreatic atrophy and pancreatic calcification were not significantly associated with PEI severity. PEI severity showed a significant improvement after PERT (P < 0.001).
Conclusions: PEI severity had significant associations with CT-based morphological severities, including severities of pancreatic duct caliber and pancreatic duct stricture or intraductal obstructing calculus. In addition, PEI-Q could be a useful indicator for evaluating therapeutic effect of PERT in clinical practice.
PP-02-317
Natural History of Infected Pancreatic Necrosis(IPN) - Prospective evaluation of management challenges in clinical practice
Asian Institute Of Gastroenterology, Hyderabad, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: One critical factor in deciding the outcome of Infected Pancreatic Necrosis(IPN) is the incorporation of an appropriate treatment strategy at the right time in the course of Acute Necrotizing Pancreatitis(ANP). We aimed to evaluate the management strategies followed using a standard treatment protocol in a cohort of IPN patients.
Methods: This was a prospective observational study that included consecutive patients of ANP with IPN from January-December 2022. Patients with IPN who didn’t improve with antibiotics were considered for drainage. Assessment for EUS-drainage with LAMS(Lumen Apposing Metal Stents) was attempted as a first preference. If EUS-drainage not feasible, percutaneous catheter drainage(PCD) was considered, as a part of step-up approach. The outcomes measured - treatment strategy adopted, timing of intervention, clinical outcome, and mortality.
Results: 117 patients were included in the study (males 62%, mean age-37.46 years). 38 patients(32%) could be managed conservatively, remaining 79(68%) patients required intervention. Initial drainage performed was EUS-guided in 30 (38%) while 49 (62%) underwent PCD. Early drainages(<4 weeks) were performed in 30/79 patients (38%), of which EUS drainage was possible in 8, while the majority (22) underwent PCD (10% vs 28%; p=0.06). The overall mortality was 20.5%. There was no significant difference in mortality between EUS drainage (22.2%) and PCD patients (30%).
Conclusion: PCD remains the primary modality both in early(< 4 weeks) and overall drainages in IPN despite efforts to consider EUS drainage. Adopting the specific modality of drainage is decided by the feasibility rather than choice in real world clinical practice.
PP-02-318
Analysis of bile acid composition in recurrent bile duct gallstone patients
Kihyun Ryu and Taehee Lee
Konyang University Hospital, Daejeon, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Abstract Body
Background/Aims: Among bile duct gallstone patients encountered in clinical practice, patients who recur after initially removing bile duct gallstone account for approximately 20%. The purpose of this study is to determine whether differences in the composition of bile acids affects the recurrence of bile duct gallstone.
Methods: From 2019 to 2022, at a single institution at Konyang University Hospital, the composition of bile from patients who underwent bile drainage for biliary tract diseases was investigated for 15 types of bile acids. Five of them are unconjugated bile acids; cholic acid (CA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA) and lithocholic acid (LCA). The remaining 10 are conjugated bile acids formed by combining with taurine and glycine.
Results: Of the total 184 patients with bile duct gallstone, 155 were in the first-diagnosis group and 29 were in the recurrent group. The mean age (years) was significantly older in the recurrent group than the first-diagnosis group (77.79 vs. 68.96, P=0.005). Among the conjugated bile acids, the quantitative values (ppm) of tauro-CA, tauro-DCA, glyco-CA, glyco-DCA, and glyco-LCA were significantly higher in the first-diagnosis group.
Conclusion: The recurrence of bile duct gallstone was significantly higher in older patients, and significant differences were shown in the composition of several bile acids. The low amount of bile acid production through the classical pathway in the recurrent group means that the classical pathway mainly occurs in the liver, so the amount of bile acid produced in the liver is especially low.
PP-02-328
Effectiveness of Saroglitazar in MASLD associated steatosis and fibrosis-An open label, real world study
Manoj Kumar Agarwal1, Sandeep Gupta1, Abhinav Agarwal2 and Chandni Agarwal1
1Belle Vue Clinic, Kolkata, India;2SMSMC, Jaipur, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Saroglitazar, a dual PPARα/γ agonist, has shown promising potential in the management of MASLD and MASH (metabolic dysfunction-associated steatohepatitis). We primarily aimed to evaluate the efficacy of Saroglitazar 4 mg OD on fibrosis and steatosis by assessing changes in liver stiffness measurement(LSM) and controlled attenuation parameter(CAP) using transient elastography(TE).
Materials and Methods: A cohort of MASLD patients (n=114, 68.4% male, 60.5% with F3-F4 fibrosis[LSM>10kPa], and mean age 51.9±12.4 years) on Saroglitazar were assessed for changes in fibrosis(LSM), steatosis(CAP), and liver enzymes at 24 and 52 weeks. The baseline mean LSM was 13±6.4 kPa, with 69(60.5%) having advanced(F3-F4) fibrosis. The paired sample t-test was used to assess changes from baseline.
Results: The study showed statistically significant improvements in LSM(kPa) by 2.23(17.2%, 13 ±6.4 to 10.8 ±4.6, p <0.05) and 3.7(28.1%, 13 ±6.4 to 9.4 ±3.7, p<0.001), in CAP(dB/m) by 30.3 (9.5%, 317.8 ±45.9 to 287.5 ±44.4, p<0.001) and 36.4(11.5%, 317.8 ±45.9 to 281.3 ±40.8, p<0.001), in ALT by 38.8 (46.6%, 83.3 ±47.3 to 44.5 ±24.8, p<0.001) and 51.7 (62.1%, 83.3 ±47.3 to 31.6 ±14.02, p<0.001), and in AST by 36.9(45.6%, 80.8 ±42.2 to 43.9 ±20.9, p<0.001) and 46.6 (57.7%, 80.8 ±42.2 to 34.2 ±12.8, p<0.001), at 24 and 52 weeks respectively.
Conclusion: Saroglitazar was found to be effective in reducing LSM and CAP, as well as liver enzyme levels SGOT and SGPT. It was also found to be equally effective in advanced stages of MASH-related fibrosis. Future well-designed randomized controlled studies will provide more strength to these findings.
PP-02-331
Increasing awareness among primary care physicians about MASLD reduced unnecessary referral rates
Eileen L Yoon1, Sang Bong Ahn2 and Dae Won Jun1
1Hanyang University, Seoul, South Korea;2Eulji University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: This study aimed to assess the impact of an online educational program on MASLD for physicians and its influence on modifying their clinical practice patterns.
Methods: A total of 869 physicians [72 physicians at referral centers and 797 primary care physicians (PCPs)], who consented to receive educational materials on MASLD, participated in this study. They completed an initial survey regarding their clinical practices for patients with MASLD, followed by a second online survey eight weeks after receiving a series of seven weekly educational materials on MASLD.
Results: Frequency of utilizing noninvasive tests for liver fibrosis, used as the first tier evaluation in both physicians at referral centers and PCPs, was low at 6.9% and 6.9%, respectively, and there was no difference between the two groups. Regarding barriers to the management of MASLD, specialists in referral centers considered the ‘short consultation time’ as the primary challenge, while PCPs cited the ‘the absence of a fee for this service’ as the major barrier. Additionally, the proportion of PCPs considering noninvasive tests testing for patients in the 'at-risk group' increased significantly for those with diabetes (32.6% to 38.5%), cardiovascular disease (9.2% to 16.1%), and ischemic stroke (8.4% to 11.5%). After education, the percentage of PCPs who immediately referred patients to a specialist after a MASLD diagnosis decreased from 15.4% to 12.3% (P value = 0.042).
Conclusions: Increasing awareness among PCPs about MASLD reduced unnecessary referral rates and an increased cardiovascular evaluation.
PP-02-332
Diagnostic performances of FIB-4 and NFS in MASLD/MAFLD in primary care clinic of Asia
Eileen L Yoon1, Sang Bong Ahn2 and Dae Won Jun1
1Hanyang University, Seoul, South Korea;2Eulji University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and aims: We aimed to explore extent to which individuals previously diagnosed with nonalcoholic fatty liver disease (NAFLD) meet the criteria fulfilled with the new nomenclature, metabolic dysfunction-associated steatotic liver disease (MASLD), within an Asian primary care cohort. Additionally, we assessed the reliability of the diagnostic performance of FIB-4 and NFS for MASLD within the primary care clinic cohort.
Methods: This retrospective cross-sectional study included participants who underwent 6,740 magnetic resonance elastography (MRE) and abdominal ultrasonography during their health checkups at nationwide health promotion centers.
Results: The prevalence rates of NAFLD and MASLD diagnosed based on ultrasonography results were 36.7% and 38.0%, respectively. Notably, 96.8% of patients in the NAFLD cohort fulfilled the new criteria for MASLD. A small proportion of patients with NAFLD (n = 80, 3.2%) did not meet the MASLD criteria. Additionally, 168 patients (6.6%) were newly added to the MASLD group. The areas under the receiver operating characteristic curves for diagnosing advanced hepatic fibrosis for FIB-4 (0.824 in NAFLD vs. 0.818 in MASLD, P = 0.891) and NFS (0.803 in NAFLD vs. 0.781 in MASLD, P = 0.618) were comparable between the MASLD and NAFLD. Furthermore, the sensitivity, specificity, positive and negative predictive value of FIB-4 and NFS for advanced fibrosis in MASLD were also comparable to those in NAFLD.
Conclusions: Almost patients (96.8%) previously diagnosed with NAFLD fulfilled the new criteria for MASLD in an Asian primary clinic cohort. Diagnostic performance of FIB-4 in the primary care MASLD cohort demonstrated satisfactory results.
PP-02-337
Anti-inflammatory effect of MLKL ATP binding inhibitor via necroptosis independent pathway in alcoholic liver disease.
Eileen L Yoon1, Sang Bong Ahn2 and Dae Won Jun1
1Hanyang University, Seoul, South Korea;2Eulji University, Seoul, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Mixed Lineage Kinase Domain Like Pseudokinase (MLKL) induces cell death through the stimulation of necroptosis. Recent studies have demonstrated that the necroptosis-independent pathway exhibits anti-inflammatory effects. Our objective is to investigate whether an MLKL inhibitor can attenuate liver disease through the necroptosis-independent anti-inflammatory pathway.
Method: Cell viability was observed following the induction of necroptosis in RAW cells. Additionally, the expression of chemokines and adhesion molecules was analyzed after inducing necroptosis in RAW cells. Compound-4, identified as an MLKL inhibitor binding to the ATP binding site, was administered to animal models with non-alcoholic fatty liver disease and alcoholic liver disease, despite lacking the ability to inhibit necroptosis.
Results: In contrast to HT29 cells, RAW cells demonstrate the ability to evade cell death in response to necroptosis stimuli. RAW cells exhibit no MLKL phosphorylation and activation after necrotic stimulation; however, they can induce necrosis-associated cell death following demethylation. While an MLKL ATP pocket binding inhibitor does not prevent cell death induced by necroptosis in RAW cells, it does reduce the expression of CXCL2, ICAM, and VCAM resulting from necroptosis. Notably, this inhibitor diminishes the expression of CXCL2, ICAM, and VCAM by inhibiting the IKKb and NFkB pathways without inducing cell death through necroptosis. Furthermore, the MLKL ATP binding inhibitor, Compound-4, demonstrated attenuation of hepatic inflammation in alcoholic animal models.
Conclusion: MLKL ATP pocket binding inhibitor has an anti-inflammatory effect through the necroptosis independent pathway in liver disease.
PP-02-339
Sorafenib Resistance in Hepatocellular Carcinoma: A Bibliometric Analysis from 2008 to 2024
1The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China;2The First Affiliated Hospital of Gannan Medical University, Ganzhou, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Resistance to sorafenib in hepatocellular carcinoma (HCC) treatment is common, and a systematic and comprehensive presentation of the research trends and hotspots in this field can assist researchers in developing more comprehensive treatment strategies.
Materials and methods: Using the keywords "hepatocellular carcinoma", "sorafenib" and "resistance", we searched the Web of Science database for relevant literature published between 2008 and 2024. Eligible articles in this field were included, and quantitative analysis and visualization were performed using bibliometric analysis software VOSviewer and Citespace. The contributions of countries, institutions, authors, and journals in this field were analyzed, as well as highly cited articles, research trends, and hotspots. Figure 1 shows the detailed information in the literature collection and analysis.
Results: A total of 1,534 eligible literatures were included in the study. The annual publication and citation counts gradually increased and reached a peak in 2022. China is the country with the largest contribution in this field, with Fudan University as the institution with the highest number of publications and Chen Kuen-Feng as the most influential researcher. Current research hotspots include cancer stem cells and stemness, hypoxia and glycolysis, autophagy and ferroptosis, tumor microenvironment, and immune checkpoint inhibitors. The research frontiers are tumor microenvironment and immunotherapy.
PP-02-340
Diagnostic Value of Multivalent Serum Aptamers for Early Hepatocellular Carcinoma
Mao-sheng Liu, Jin-Ke Wang, Ding-Fan Guo, Qi Wen, Yun-Hui Liang, Ting Wang and Kun-He Zhang
The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: The current blood-based diagnostic methods for early-stage hepatocellular carcinoma (eHCC) are inadequate for clinical application due to their suboptional performance. Previously, we generated a group of aptamers against HCC sera. In this study, we aimed to evaluate the diagnostic value of nanoparticle-based multivalent HCC serum aptamers for eHCC.
Materials and methods: Serum samples and clinical data were collected from eHCC patients (n=104), chronic liver disease patients (cirrhosis and hepatitis) (n=184), and healthy controls (n=96) at our hospital. Using our previously developed aptamer-based triple serum fluorescence intensity (ATSFI) assay, we sequentially measured the autofluorescence of 5μL serum, the fluorescence after adding 5μL EvaGreen dye (16×), and the fluorescence after adding 5μL multivalent HCC serum aptamers (0.2pmol/μL). The diagnostic performance of the original and derived fluorescence indicators for eHCC was assessed using the area under the receiver operating characteristic curve (AUROC).
Results: The highest AUROCs among single fluorescence indicators were 0.863 for distinguishing eHCC from chronic liver disease, 0.919 for distinguishing eHCC from healthy controls, and 0.887 for distinguishing eHCC from non-HCC individuals (chronic liver disease and healthy controls). The AUROC of AFP was 0.732 for eHCC vs. non-HCC. Subgroup analysis showed AUROCs of 0.903, 0.889, and 0.907 for distinguishing AFP-negative eHCC, small eHCC, and poorly differentiated eHCC from non-HCC individuals, respectively. Logistic stepwise regression modeling using the ATSFI indicators yielded corresponding AUROCs of 0.936, 0.948, and 0.950, respectively.
Conclusion: Multivalent HCC serum aptamers are valuable for the diagnosis of eHCC, including AFP-negative, small, and poorly differentiated eHCCs.
PP-02-341
A Comparison of Safety and Efficacy of Elobixibat and Lubiprostone in Chronic Constipation:A Systematic Review
Yulisa Tanjung
Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, M, Medan, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
A Comparison of Safety and Efficacy of Elobixibat and Lubiprostone in Chronic Constipation: A Systematic Review
YULISA Astari Tanjung [1]; MASRUL Lubis [2]; IMELDA rey [3]; TAUFIK Sungkar [4]; ILHAMD Ilhamd [5]; DARMADI Darmadi [6]
1-Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; 2-Division of Gastroenterohepatology, Department of Internal Medicine
Abstract
Objectives: Constipation is one of major gastrointestinal disorders worldwide. Chronic constipation impairs patients' quality of life. Previous treatment options for chronic constipation show unsatisfactory outcome. This systematic review aims to compare efficacy and safety between elobixibat and lubiprostone in patients with chronic constipation.
Materials and methods: Literature searching was conducted in multiple databases. Inclusion criteria were studies involving patients with chronic constipation, utilization of elobixibat or lubiprostone, and reporting efficacy and safety outcomes. Data was analyzed using Microsoft Excel software.
Results: A total of 6 studies with 2,949 patients was included in this study. A total of 3 studies reported symptom’s improvement after administration of lubiprostone compared to placebo while 2 studies showed similar findings after administration of elobixibat. Adverse events were less common in elobixibat compared to lubiprostone.
Conclusion: Elobixibat and lubiprostone are effective in treating chronic constipation. Elobixibat shows superiority in safety profile compared to lubiprostone.
Chronic Abdominal Pain Due to Liver Infarction Initially Suspected as Hepatoma : A Rare Case
Nyimas Maida Shofa1, Tri Asih Imro'ati2, Budi Widodo1 and Titong Sugihartono1
1Division of Gastroentero-Hepatology, Department of Internal Medicine, Airlangga University – Dr. Soetomo General Hospital, Surabaya, Indonesia;2Department of Internal Medicine University Airlangga Hospital, Surabaya, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Introduction: Liver infarction are extremely rare because the liver receives blood from various sources. Liver infarction has an unknown cause. The lack of distinct imaging appearances and clinical signs typically results in a misdiagnosis and poor prognosis. It is unclear how best to treat these infarctions once they are diagnosed
Case Description: A 66-year-old man complained of persistent pain in the right upper abdomen, nausea and appetite loss. Abdominal ultrasonography demonstrated irregular edge hypoechoic nodules size 2.2cm in diameter with suspected hepatoma. Liver function and virus marker tests within normal limits. Computed tomography examination resulted in a 2.4x3.6x1.6 cm hypodensities lesion in the 6th segment of the right lobe of the liver which, when given contrast, was neither wash in nor wash out contrast on the intralesion. Seen arterial enhancement on in the extralesion, consistent with hepatic infarction. Anticoagulant therapy was chosen as the treatment strategy. Patients were given rivaroxaban. Swift resolution of the abdominal pain was noted within a month. The patient continued treatment for six months
Discussion: The causes of liver infarction are complex, and can even be fatal. Simple imaging, such as ultrasound, CT scan, or magnetic resonance imaging, is sometimes insufficient. In addition, detailed history taking, physical examination, and related serum studies are also crucial. We should also be aware of the clinical characteristics, imaging appearance, and serum results of other liver lesions, such as liver cancer. Anticoagulant therapy can be considered in the treatment of liver infarction of unknown etiology.
PP-02-343
Efficacy of Saroglitazar on Liver fibrosis in MASLD:An open label real world study
Sujit Chaudhuri and Agnibha Dutta
Manipal Hospital, Broadway, Kolkata, India
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Liver stiffness measurement (LSM) by transient elastography(TE) is a valuable tool in the non-invasive assessment and management of liver fibrosis in chronic liver diseases(CLD). Saroglitazar, a dual PPARα/γ agonist, is approved in India for management of MASLD(metabolic dysfunction associated Steatotic liver disease)with evidence of fibrosis. We evaluated the effect of Saroglitazar in different fibrosis stages of MASLD.
Materials and Methods: 133 documented MASLD patients on Saroglitazar treatment(male 70.3%(92), non-diabetic 52.3%(68), non-hypertensive 46.1%(59), non-dyslipidemic 71.2%(89), 38.3%(51) with LSM>14 kPa) were evaluated for changes in mean liver stiffness(LSM) and steatosis (CAP) from baseline to follow-up visits till 96 weeks. Paired sample t-test at 95% CI was applied and p<0.05 is considered as statistical significant. Safety was assessed at every follow up visit based on the frequency and severity of any adverse events.
Results: The mean improvement in LSM was 6.9kPa(42.6%,p<0.001) from 16.2±12.4 to 9.3±4.8 and in CAP was 59.7dB/m(19.3%,p<0.001) from 309.6±48.9 to 249.8±49.8, after 96 weeks. In cohort analysis based on fibrosis stages(F0-F4), mean LSM improvement in cohort1(G1,LSM<7.5kPa(F0-F1),n=20) was 0.61(9.3%,p=0.03) from 6.5±0.68 to 5.9±1.04; in cohort2(G2,LSM7.5-10kPa(F2),n=39) was 1.73(20.1%,p<0.001) from 8.7±0.67 to 6.9±2.6; in cohort3(G3,LSM10-14kPa(F3),n=23) was 3.35(28.1%,p<0.001) from 11.95±0.9 to 8.6±3.7; and in cohort4(G4,LSM>14kPa(F4),n=51) was 15.3(54.6%,p<0.001) from 28±13.3 to 12.7±5.3.There was no major side effect during the treatment except 2 brief incidences (skin rash and loose motion).
Conclusion: Saroglitazar is found to be effective in fibrosis reduction across the stages from F0-F1 to F4 in MASLD. Further well controlled randomized studies are needed to support the finding of this study.
PP-02-344
Early and Late-Onset Colorectal Cancer in African Americans During COVID-19
Hassan Brim1, Lakshmi Chirumamilla1, Challa Suryanarayana Reddy1, Adeyinka Laiyemo1, Reza Oskrochi, Mrinalini Deverapall1, Rumaisa Rashid, Rabia Zafar1 and Mudasir Rashid1 and Babak Shokrani1 and Hassan Ashktorab1
1Howard University, Washington, United States;2American Middle East University, Kuwait
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: The incidence of early-onset colorectal cancer (EOCRC, <45 years of age at onset) is on the rise among adults, including African Americans (AA).
Aim: To examine differences between EOCRC and late-onset colorectal cancer (LOCRC) among AA patients and any effect during COVID by comparing data during pre-covid (2015-2019) and the COVID era (2020-2023).
Methods: We conducted a retrospective review of Howard University Hospital records from 2015 to 2023 for CRC patients that included demographics, clinicals, pathology, and colonoscopy records. A three-year interval analysis was performed to compare post-COVID era (2020-2023) to preceding years to discern temporal trends.
Results: 138 LOCRC and 13 EOCRC cases of which >80% of patients were AA. Compared to pre-COVID, LOCRC cases increased in number from 55 to 83, and EOCRC cases increased from 6 to 7 during COVID. There was no change in mean age for LOCRC (64.7 vs. 65.3 years) but mean age increased for EOCRC (37.3 vs. 41.5 years). Males predominated in both groups particularly during the pandemic. More than 65% of LOCRC patient colonoscopies were for diagnostic purposes. GI bleeding as a colonoscopy indication and reduced bowel preparation quality were increased during the pandemic. EOCRC patients showed a shift from stage 4 (49.2%) to stage 2 (30%) and LOCRC patients staging trends changed from stage 4 (40%) to stage 3 (28.6%).
Conclusion: We report increase in CRC cases during the COVID-19 era, especially among young AA males. EOCRC and LOCRC patients showed distal location predominance, most commonly in recto-sigmoid region.
PP-02-345
Comparative diagnostic performance of RUT with the sweeping versus conventional method after Helicobacter pylori eradication
Kee Lee and Jae Keun Kim and Choong Kyun Noh and Kil Ho Lee and Byung Moo Yoo
Ajou University School Of Medicine, Suwon, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background and Aims: The rapid urease test (RUT) is widely used to detect Helicobacter pylori (H. pylori) infection; however, it is not preferred as a monitoring strategy following eradication owing to its low sensitivity. In this study, we evaluated the diagnostic performance of RUT using the sweeping method, which overcomes the limitations of conventional tissue sampling methods following eradication.
Methods: Patients who received H. pylori eradication treatment were enrolled. Each of the sweeping and conventional methods was performed on the same patients to compare diagnostic performance. Urea breath test (UBT), histology, and PCR were performed to determine true infection. Logistic regression analysis was conducted to investigate reasons for discrepancies between the results of the two methods.
Results: In 216 patients, the eradication success rate was 68.1%, sensitivity and specificity of the sweeping method were 0.812 and 0.912, respectively, whereas those of the conventional method were 0.391 and 0.993, respectively (P<0.05 for all). AUROC for the sweeping method was higher than that for the conventional method (0.862 vs. 0.692; P<0.001). The mean time to H. pylori detection for the sweeping method was 4.7±4.4 min and 12.3±16.1 min for the conventional method (P<0.001). The risk for inconsistent results between the two methods was the highest in the UBT value 1.4–2.4‰ (OR 3.8, P=0.016).
Conclusions: The RUT with the sweeping method could potentially replace the tissue sampling method as a test to confirm H. pylori eradication and be an alternative option to UBT for patients requiring endoscopy.
PP-02-346
Slc26a9 governs gastric parietal cell identity and has an important role in the injury-induced metaplasia
Bei Ji1, Zhiyuan Ma1, Zilin Deng1, Enqing Wu1, Jiaxing Zhu1, Biguang Tuo1, Taolang Li2 and Xuemei Liu1
1Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China;2Department of general surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Gastric injury induces metaplasia is based on parietal cells loss and reprogramming chief cells into proliferative, mucin-rich spasmolytic polypeptide-expressing metaplasia (SPEM) cells. Slc26a9 is a key regulator to maintain mucosal homeostasis. However, the exact role of Slc26a9 in injury-induced metaplasia is need further investigated.
Methods: PCR and histopathological and immunofluorescence (IF).
Results: We first demonstrated that Slc26a9 is expressed in all early gastric progenitors and strongly expressed in mature parietal cells. In addition mice with selective Slc26a9 deficiency in parietal cells exhibited SPEM based on autoimmune atrophic gastritis and significant up-regulation of parietal death-related markers including GSDMD, Caspase- 1, ASC, IL-1β, IL-18, and NLRP3 expression, with activated GSDMD leading to mitochondrial mediators of ACO2 leading to the death of parietal cells, followed Tuft cell migration and release of the alerting hormone IL-25, which activates ILC2 cells and promotes the activation of the IL-25-ILC2-M2 macrophage axis, resulting in the development and progression of SPEM. Also in human SPEM tissues, Slc26a9 significantly downregulated and the IL-25-macrophage M2 polarization marker CD163+ was significantly upregulated. These results suggest that deletion of the gastric parietal cell-specific Slc26a9 gene leads to parietal cell death by regulating GSDMD-mediated mitochondrial damage; and the development of SPEM through the modulation of IL-25-ILC2-M2-type macrophage polarization.
Conclusions: We identify Slc26a9 as a potential key regulatory gene for parietal cells. Slc26a9 plays an essential protective role in stabilizing gastric mucosal immunity and gastric epithelial cell homeostasis during injury-induced chemotaxis.
PP-02-347
Comparison of Synergistic Sedation With Midazolam and Propofol Versus Midazolam and Pethidine in Colonoscopies
Hyun-soo Kim, Dong Hyun Kim, Jae Woong Lim, Min Jae Kim and Gang Han Lee
Chonnam National University Hospital And Medical School, 42, Jebong-ro, Dong-gu, Gwangju, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background: Colonoscopy is crucial for the early detection of colorectal cancer. However, discomfort associated with this procedure often necessitates sedation, and the optimal sedation method remains unclear.
Methods: We conducted a prospective randomized controlled study in which patients undergoing colonoscopy were randomly assigned to one of two treatment regimens. Group A received midazolam and propofol, while group B received midazolam and pethidine.
Results: A total of 51 patients were included in the analysis, with 23 and 28 patients in groups A and B, respectively. Adverse events did not significantly differ between the two groups. Additionally, there were no differences in cecal intubation and total procedural times. However, group A demonstrated a lower frequency of postural changes (1.0±0.7 vs. 1.5±0.7, p=0.02) and a reduced rate of manual compression (52.2% vs. 82.1%, p=0.02). There were no significant differences between the two groups in terms of subjective pain or satisfaction.
Conclusions: Both sedation methods demonstrated similar safety profiles and satisfactory outcomes. The combination of midazolam and propofol showed comfortable insertion by minimizing the need for patient repositioning and manual compression during colonoscopy.
PP-02-348
Outcomes of Colorectal Endoscopic Submucosal Dissection According to the Size of Colorectal Neoplasm
Hyun-soo Kim and Dong Hyun Kim
Chonnam National University Hospital And Medical School, 42, Jebong-ro, Dong-gu, Gwangju, South Korea
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Background/Aim: Endoscopic submucosal dissection (ESD) is a valuable technique for treating early colorectal neoplasms. However, there are insufficient data concerning the treatment outcomes in relation to the size of colorectal neoplasms.
Patients and Methods: The data on ESD for colorectal epithelial neoplasms between January 2015 and December 2020 were retrospectively collected from five tertiary medical centers. Colorectal neoplasms were stratified into groups based on their longitudinal diameter: <20 mm as Group 1, 20–39 mm as Group 2, 40–59 mm as Group 3, and 60 mm or more as Group 4.
Results: Of the 1,446 patients, 132 patients were in Group 1 (<20 mm), 1,022 in Group 2 (20–39 mm), 249 in Group 3 (40–59 mm), and 43 in Group 4 (≥60 mm). There was an observed trend of increasing age from Group 1 to Group 4, accompanied by a corresponding increase in the Charlson Comorbidity Index. Procedure time also exhibited a gradual increase from Group 1 to Group 4. Similarly, the length of hospital stay tended to increase from Group 1 to Group 4. The predictive model, using restricted cubic spline curves, revealed that as the size of lesion exceeded 30 mm, complete resection steadily decreased, and major complications notably increased.
Conclusion: As the size of colorectal neoplasms increases, the rate of complete resection decreases and the rate of complications increases.
PP-02-349
Unusual cause of upper Gastro-Intestinal Bleeding with gastric and duodenal ischemia: A case report
Dalouny Sihalath1, Sengdao Vannarath1 and Thitsamay Luangxay2
1Mahosot Hospital, Vientiane, Lao People's Democratic Republic;2National Cancer Center Laos, Vientiane, Lao People's Democratic Republic
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Idiopathic Catastrophic Anti-Phospholipid Syndrome (CAPS) is a rare condition with multiple complications to end organ. We report a rare case of CAPS, a 58-year-old Laotian female presented to the emergency department with coffee ground emesis for 2 days and postprandial abdominal fullness for 3 weeks. Physical examination revealed mildly abdominal tenderness. The patient was hemodynamically stable. Laboratory findings were total white blood cell was normal, hemoglobin level of 9.7 g/dL, platelet count of 238,000/cu.mm, normal creatinine level, Prothrombin Time was 18 seconds with an INR level of 1.1.
Esophagogastroduodenoscopy revealed an edematous purplish discoloration of the mucosa with multiple subepithelial hemorrhages from the proximal gastric body to second part of the duodenum. Abdominal computed tomography showed thickening of gastric and duodenum mucosa walls and decreased enhancement of the wall without pneumatosis. Multiple sites of thrombosis were found at the gastro-duodenal and splenic arteries, superior mesenteric vein thrombosis, splenic vein, and splenic infarction. Further findings included of a C-Reactive protein 95.018 mg/dL, and work-up for hypercoagulable states such as Anticardiolipin antibodies and lupus anticoagulant were normal. Additionally, previous use of oral contraceptive drugs was noted in the patient’s history.
The final diagnosis was acute gastro-duodenal ischemia due to suspected Idiopathic CAPS and managed with bowel rest, parenteral nutrition, and conservative with Anticoagulant therapy. She gradually improved abdominal pain. Although, CAPS might be rare a medical condition, it can manifest as a bleeding complication and could be fatal. More in-depth study needs to be conduced this syndrome has scarce information.
PP-02-350
ASSOCIATION BETWEEN ENDOSCOPY FINDINGS AND THE DEGREE OF INFLAMMATION FROM BIOPSY RESULTS IN GASTRITIS PATIENTS
I Komang Wisuda Dwija Putra, I Ketut Mariadi, Gde Somayana and Komang Agus Wira Nugraha
Rsup I.G.N.G Prof Ngoerah, Denpasar, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Gastritis is an inflammation of the gastric mucosa which can be acute or chronic. Gastritis may be erosive (superficial, deep, or hemorrhagic) or non-erosive. Endoscopy and stomach tissue biopsy are the most accurate screening method in assessing the severity of gastritis and evaluating the risk of metaplasia. Level of inflammatory cell infiltration and Helicobacter pylori infection proven to be related to increased risk of gastric cancer. By knowing the pattern of severity gastritis in patients, then the level risk of stomach cancer in an area can be predicted. Objectives: evaluating the association between endoscopy findings and inflammation level in gastritis patients.
Materials and methods: This study uses a retrospective cohort design. Subjects were sampled using consecutive random sampling. Patients with gastritis diagnosis, age 18 - 80 were included in this study. The subject's biopsy material was taken from endoscopy examination. Univariate analysis was carried out to measure the frequency distribution. Bivariate analysis was carried out using chi square test.
Results: The average age of the subjects was 47.08 ± 14.46 years old with a range of 19 – 79 years old. There was no statistically significant relationship between degree and activity level, H.pylori, atrophic condition, intestinal metaplasia, inflammation level with a p value of 0.353; 0.267; 0.142; 0.512; 0.864. There was also no statistically significant relationship between location and inflammation level with a p value of 0.479.
Conclusion: There were no significant relationship between endoscopy findings and the degree of inflammation from biopsy results in gastritis patients.
PP-02-351
Fecal Bifidobacterium is Associated with Improvement of Liver Fibrosis and Survival in Virus-related Liver Cirrhosis
Komang Agus Wira Nugraha1, I Ketut Mariadi1, Gde Somayana1 and Cokorde Istri Yuliandari Krisnawardani Kumbara2
1Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia;2Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Badung, Indonesia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objective: Imbalance of gut microbiota, has been linked to inflammation and the progression of liver diseases. Investigating the function of the Bifidobacterium in liver cirrhosis holds promise for identifying therapeutic targets that could revolutionize the management of liver cirrhosis and improve the survival. This study aimed to investigated the role of fecal Bifidobacterium in improvement of liver fibrosis and survival.
Materials and Methods: This prospective cohort study recruited virus-related liver cirrhosis patients in Prof. Dr. I.G.N.G. Ngoerah Hospital from May 2021. All subjects received antiviral therapy for Hepatitis B or C. The fecal Bifidobacterium (CFU/g) was examined using DNA isolation and then categorized as decreased (<0.78 CFU/g) and normal-increased (≥0.78 CFU/g). Liver fibrosis (kpA) was determined using transient elastography. After 6 months of follow up, we evaluated the improvement of liver fibrosis and survival.
Results: A total of 78 patients were enrolled in this study, and the mean age of subjects were 51.37 years (SD 11.87). The Logistic Regression analysis showed that normal-increased Bifidobacterium was associated with improvement of liver fibrosis (AOR: 11.19, 95% CI: 3.58-34.93, p<0.001). The Kaplan-Meier curve revealed that the median survival of subjects with a normal-increased Bifidobacterium (22 weeks, 95% CI 20.24-23.75) was higher than the subjects with a low Bifidobacterium (12 weeks, 95% CI 9.15-14.84)(p=0.003). A normal-increased Bifidobacterium also had an association with survival (HR: 0.084, 95% CI 0.011-0.66, p=0.019), after adjustment to confounding variables in Cox Regression analysis.
Conclusion: The normal-increased level of Bifidobacterium is associated with improvement of liver fibrosis dan survival.
PP-03-001
Choledochal cyst type ii with cystolithiasis managed with ercp and papillotomy in an adult male
Luigi Dan Alit and Ike Minerva
West Visayas State University Medical Center, Iloilo City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Choledochal cyst is a rare biliary tree anomaly, with type 2 comprising only 2% of the different types. It usually presents during childhood and is four times more common in females. Associated complications are cystolithiasis, pancreatitis, and malignant transformation. Simple excision or diverticulectomy is the treatment approach. In this case, work up of an adult male showed choledochal cyst with intracystic stones successfully managed with ERCP and papillotomy.
Case description: A 33-year-old, male, occasional alcoholic beverage drinker consulted due to one month epigastric pain associated with nausea. No reported fever, jaundice, or palpable mass. Initial work up revealed mild leukocytosis and slightly elevated bilirubin but with normal serum amylase, alkaline phosphatase, and aminotransferases. Initial work up using whole abdomen ultrasound revealed acalculous cholecystitis and non-obstructing right nephrolithiasis. Further imaging studies with abdominal CT scan considered choledochal cyst type II and pancreatic head cyst. MRCP and ERCP confirmed the presence of choledochal cyst with intracystic stones. Papillotomy and biliary stent placement were performed for biliary drainage. Biliary exploration with possible transduodenal excision of cyst was contemplated prior to discharge. On reevaluation after eight months, ERCP showed no choledocholithiases nor distal CBD cyst lithiases.
Discussion: Choledochal cysts type II is a rare biliary duct anomaly, more so in adults. They may present with nonspecific manifestations which may delay diagnosis, thus, a high index of suspicion is needed. Complications such as cystolithiasis and pancreatitis may necessitate papilla sphincterotomy and/or biliary drainage while awaiting definitive management.
PP-03-002
Metastatic Renal Cell Carcinoma Presenting as Gastrointestinal Bleeding: A Case Report
Vivien Ang and Porciuncula Vicente II
St. Luke’s Medical Center Quezon City, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Renal Cell Carcinoma (RCC) is infamous for its propensity for widespread metastasis. However, metastasis to the gastrointestinal tract discounting the liver, is extremely rare, comprising only 0.2-0.7% of cases.
Case description: This is a case of a 44-year-old female who was recently diagnosed with Renal Cell Carcinoma Stage IV (Metastatic to the Lungs, Bones, Lymph Nodes, Adrenals and Ovary by PET-CT scan) presenting with hematochezia. Patient underwent esophagogastroduodenoscopy showing multiple gastric polyps with central ulceration and duodenal ulcers (Forrest Class III), as well as a colonoscopy revealing non-bleeding colon ulcers at the ileocecal valve and ascending colon. Biopsies all revealed ulcerating poorly differentiated carcinoma. Immunohistochemical staining was requested and was positive for Cytokeratin, PAX8, CD10, Vimentin and WT-1, which are compatible with Renal Cell Carcinoma. The patient was managed with blood transfusions and was subsequently started on Lenvatinib.
Discussion: RCC is a primary renal malignancy which accounts for an estimated 3% of all adult cancers. Metastasis is uncommon in the gastrointestinal tract, with less than 30 cases reported in literature. Colon involvement is rarer compared to the stomach and small bowel- all of which are present in our patient’s case. Symptoms for gastrointestinal tract involvement are mainly gastrointestinal bleeding and iron deficiency anemia. Management options for gastrointestinal metastatic lesions include endoscopic resection, embolization, chemotherapy and immunotherapy, but optimal treatment remains controversial. Our case outlines the importance of careful endoscopic evaluation and histologic examination of RCC patients presenting with gastrointestinal bleeding, since although rare, metastasis should be a consideration.
PP-03-003
Hereditary Hemorrhagic Telangiectasia in an Elderly Filipino Female: A Rare Case of Gastrointestinal Bleeding
Ferdinand Anzo and Edgardo Bondoc
St. Luke's Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Hereditary hemorrhagic telangiectasia (HHT) or Osler-Weber-Rendu syndrome is a rare genetic disorder characterized by small vascular lesions in the oral cavity, lips, fingers, and mucosa of the nasal cavity and gastrointestinal tract. Due to its systemic complications, a multidisciplinary management approach is essential. This case report will discuss a patient with multiple telangiectasia following a clinical diagnosis of HHT, based on the Curaçao diagnostic criteria.
Case Presentation: This is the case of an 81-year-old Filipino female, known hypertensive, who was admitted due to anemia presenting with epistaxis and melena. Upon physical examination, multiple punctiform telangiectasia on the lips, oral mucosa, and hands. Gastroscopy revealed multiple arteriovenous malformations lacking intervening capillaries in the esophagus, stomach, and duodenum. Argon plasma coagulation was performed. The patient kept NPO while receiving Pantoprazole 40mg IV twice daily and was gradually reintroduced to a diet 24hour post-procedure. On hospital day 4, she was discharged and advised to follow up after one week with a recommendation to undergo repeat gastroscopy.
Conclusion: Despite not undergoing genetic testing due to a lack of funds, patients present with abnormal blood vessels in several areas of the body (such as the lips, tongue, hands, esophagus, stomach, and duodenum) along with signs of bleeding (epistaxis and melena). This presentation is consistent with HHT as defined by the Curaçao criteria. Additionally, having first-degree relatives with the same condition constitutes the classic triad of the syndrome; however, it is unknown whether the patient is the first in their family to have this syndrome.
PP-03-004
Insulinoma Diagnosis: Uncovering a Rare Pancreatic Lesion
Ferdinand Anzo, Ronald Vallar and Jan Axel Yusi
St. Luke's Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Insulinoma is an uncommon pancreatic neuroendocrine tumor characterized by disproportionately high levels of insulin or C-peptide during a 72-hour fast-induced hypoglycemia. Diagnostic techniques commonly used to identify pancreatic tumors include CT, MRI, and EUS. Surgical excision are preferred treatments for solitary tumors. This study presents a rare case of insulinoma detected via EUS, despite normal findings on CT and MRI.
Case Presentation: This is a 74-year-old female with a history of Multinodular toxic goiter, who presented with persistent hypoglycemia, accompanied by loss of consciousness and subsequent recovery following intravenous glucose administration. Work-up revealed elevated C peptide (8.28 ng/mL) and fasting insulin (281.90 uU/mL). A whole abdominal CT scan showed unremarkable findings. A subsequent abdomen MRI revealed a small hepatic and renal cyst, with the pancreas appearing unremarkable. Despite these findings, due to the persistence of hypoglycemia, an EUS was performed, revealing a 3.5 mm hypoechoic to almost anechoic well-defined lesion at the pancreatic body. FNAB confirmed a round cell neoplasm. Immunohistochemical staining showed positivity for Synaptophysin, Chromogranin, with a Ki67 index of 1.3% and retained nuclear expression of SMAD 4. The patient subsequently underwent pancreatic enucleation, which confirmed a neuroendocrine tumor. Following surgery, the patient has reported no further episodes of hypoglycemia.
Conclusion: In summary, for suspected cases of insulinoma, imaging techniques like CT and MRI are initial steps, but invasive modalities such as EUS are notably superior for localizing lesion. Laparoscopic enucleation is the preferred surgical technique, ensuring complete removal of the tumor capsule to prevent recurrence.
PP-03-005
Neuroendocrine Tumor presenting as cystic lesion in the Liver and Pancreas
Ferdinand Anzo, Juliet Cervantes and Jessika Iza Chua
St. Luke's Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Neuroendocrine tumors are indeed uncommon compared to other types of tumors, but they can occur in various organs, including the pancreas and liver. While solid masses are predominantly common presentations with higher incidence in males, cystic forms do occur, though less frequently. Neuroendocrine tumors of the pancreas and liver to be discussed underscores the challenge in diagnosing and determining the specific location of tumor origin involving an adult female presenting with abdominal pain.
Case Presentation: A 47-year-old female presented with intermittent right upper quadrant abdominal pain initially managed as cholecystolithiasis. Medications provided partial relief, leading to hepatobiliary ultrasound revealing an enlarged right liver lobe with a 19.9 x 18.3 x 18.4 cm complex mass and another 12.2 × 13.9 × 11.1 cm mass in the mid upper abdomen. Laboratory tests showed normal liver function but elevated lipase (3x) and CA19-9 (55.36). CT scans revealed hepatomegaly with cirrhotic changes and multiple peripherally enhancing hypodense lesions in the liver, and an atrophic pancreas with a large, complex cyst. Aspiration and drainage were performed, with negative cultures and cytology. She underwent exploratory laparotomy and wedge resection confirming a Grade 2 Well-Differentiated Neuroendocrine Tumor
Conclusion: Cystic neuroendocrine tumors of the pancreas and liver are rare and pose diagnostic challenges, often overlooked due to nonspecific symptoms, leading to delayed diagnosis and treatment. Prognosis varies based on factors such as tumor grade, stage at diagnosis, presence of metastasis, and hormone secretion status (functional vs. non-functional).
PP-03-006
Clinical Outcomes of Achalasia after Per-oral Endoscopic Myotomy from a Tertiary Hospital in the Philippines
Ferdinand Anzo and Marc Julius Navarro
St. Luke's Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Achalasia is an uncommon neurogenic esophageal motility disorder characterized by incomplete relaxation of the lower esophageal sphincter, coupled with the absence of organized peristalsis. POEM is a hybrid technique for treating achalasia and preferred and current endoscopic treatment of choice due to lower complication rate compared to laparoscopic Heller myotomy. Since POEM has only been introduced to the Philippines recently, there are few centers who offer this procedure. So in this study, we will focus on the patients who underwent POEM and their clinical outcomes.
Case Presentation: Each of the five female patients sought consultation for dysphagia associated with typical clinical manifestation of esophageal motility disorder. All patients underwent EGD and high resolution esophageal manometry (HREM) as part of the pre evaluation of POEM. It revealed retained food particles in the esophageal lumen, dilated esophagus with narrowing of the esophagogastric junction (EGJ) with puckered appearance and type 1 achalasia in HREM. A barium esophagogram was also done to all patients with consistent findings of achalasia. Pre-POEM and Post-POEM Eckardt score with reflux symptoms were evaluated using GERDQ system with positive outcomes. All patients who underwent repeat EGD noted a good myotomy site and the EG junction. No complications were noted for all patients after POEM.
Conclusion: Achalasia is a rare motility condition. There is a good clinical outcome of patients diagnosed with achalasia after POEM in spite of different variables. All five subjects showed symptoms improvement and weight gain underscoring POEM’s important role in the management of achalasia.
PP-03-007
The Effectiveness of Left Lateral Decubitus Sleeping Position in Gastroesophageal Reflux Disease (GERD): An EBCR
Fhathia Avisha and Rima Karlina
Department Of Internal Medicine, University Of Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: GERD is a disorder in which gastric contents experience repeated reflux into the esophagus, causing disturbing symptoms and complications.
Objective: To determine the effectiveness of non-pharmacological therapy for left lateral decubitus (LLD) and right lateral decubitus (RLD) and supine sleeping positions on reflux symptoms in adult patients with GERD.
Methods: A literature search was conducted on five databases, namely Pubmed, Cochrane, Scopus, and Sciencedirect on February 19, 2024 using the keywords “Gastroesophageal Reflux Disease,” and “Sleep Position” or “Left Lateral Decubitus” or “Right lateral Decubitus or Supine AND nocturnal reflux symptoms or Symptoms”. There were seven studies on Pubmed, no study on Cochrane, one study on Scopus, and eighteen studies on Sciencedirect. The articles found were screened based on the inclusion and exclusion criteria.
Results: There were significant differences in acid exposure time (AET) and acid clearance time (ACT) in patients with LLD sleep position compared to RLD and supine sleep position. Two non-randomized studies showed decreased AET and ACT in LLD sleep position compared to RLD (mean difference [MD] -2,03 [95% CI: -3,62 to -0,45]; -81,84 [95% CI: - 127,48 to -36,20],) and supine position (MD -2,71 [95% CI: -4,34 to -1,09]; -74,47 [95% CI: - 116,26 to -32,69], respectively).
Conclusion: LLD sleeping position significantly reduced acid exposure time and acid clearance time among patients with GERD compared to RLD and supine sleeping positions. LLD sleep position is recommended for GERD patient to reduce reflux symptoms.
Keywords: Sleep positions, Left Lateral Decubitus, Nocturnal Gastroesophageal Reflux.
PP-03-008
Recurrent severe anaemia related to rare oesophageal and gastric lymphangioma treated with haemostatic radiation therapy
Muhammad Khifzhon Azwar, Amalia Nur Pratiwi, Leonard Nainggolan, Amanda Pitarini Utari and Saskia Aziza Nursyirwan
Faculty Of Medicine, Universitas Indonesia - Department Of Internal Medicine, Cipto Mangunkusumo Hospital, Jakarta Central Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Review of cases published in English in electronic databases showed only 30 cases of oesophageal lymphangioma and 24 cases of gastric lymphangioma ever reported. We aimed to report successful management of oesophageal and gastric lymphangioma case presenting with recurrent severe microcytic anaemia due to gastrointestinal bleeding and iron deficiency.
Case Description: A 21-year-old lady came with chief complaint of worsening fatigue since 3 days before admission. Vomiting of fresh blood preceded the worsening of fatigue, accompanied by epigastric pain and passing black stools. The complaints have recurred since 3 years before admission. We observed malnutrition and unilateral preaxial polydactyly of the upper extremity. Full blood count on admission often showed haemoglobin levels between 2 and 4 g/dL. Fatigue episodes improved with packed cells transfusions. Oesophagogastroduodenoscopy showed proliferative mass starting from distal oesophagus, extending to oesophagogastric junction and gastric cardia. Pathological examinations suggested gastric polyp, as well as gastric and oesophageal lymphangioma. Combination of dietary modification per nasogastric tube, packed cells transfusion, iron repletion, and symptomatic treatments did not result in lasting improvement. Patient received 10 fractions of 2 Gy haemostatic intensity-modulated radiation therapy (IMRT) and subsequently achieved two-month clinical, radiological, and laboratory-based improvements.
Discussion: Anaemia and gastrointestinal bleeding were considered rare clinical presentations of oesophageal and gastric lymphangioma. There may be genetic component contributing to the occurrence of lymphangioma in our case report patient. Haemostatic IMRT is proven to be safe and effective for this case.
PP-03-009
Hepatitis A presenting with Acute Liver Failure and Severe Acute Pancreatitis: A rare case report
Rajat Bansal1, Hitendra Garg1, Mohammad Irtaza1, Arun Kumar2, Sudeep Khanna1 and Neerav Goyal2
1Department of Gastroenterology and Hepatology, Indraprastha Apollo Hospital, New Delhi, India;2Department of Liver Transplant, Hepatobiliary and Pancreatic Surgery, Indraprastha Apollo Hospital, New Delhi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Acute liver failure (ALF) and severe acute pancreatitis (SAP) are rare but serious complications of hepatitis A virus (HAV) infection, particularly in adolescents. This report presents a case of a young male diagnosed with both ALF and SAP secondary to acute HAV infection.
Case Report: A 17-year-old male presented with fever for three days followed by severe abdominal pain, vomiting, jaundice, and altered mental status for two days. Investigations showed elevated liver enzymes, hyperbilirubinemia, coagulopathy, and raised ammonia levels. Serum lipase, amylase were markedly elevated, confirming acute pancreatitis. HAV IgM antibodies were positive. Imaging showed hepatomegaly without biliary obstruction and diffuse pancreatic inflammation with peripancreatic collection. He had encephalopathy and respiratory failure and was intubated and managed in ICU with supportive therapy, N-acetyl-cysteine, and CRRT. Due to worsening sepsis, percutaneous drainage of peripancreatic collection was done. He later underwent retroperitoneal debridement and necrosectomy for infected peripancreatic collections. He gradually improved and was discharged in stable condition.
Discussion: Hepatitis A typically causes self-limiting illness, but rarely, it can lead to ALF and SAP. ALF occurs in less than 1% HAV cases. Acute pancreatitis has been reported to occur mostly without fulminant hepatitis (94%) in HAV and is severe in 9% cases. The pathophysiology is not fully understood. HAV is proposed to cause ALF from an overwhelming immune-mediated response leading to massive hepatocyte necrosis and pancreatitis due to immune-mediated mechanisms, direct viral injury, and ampullary edema causing pancreatic fluid outflow obstruction. Early recognition and supportive management are essential for favorable outcome.
PP-03-010
Pediatric recurrent acute pancreatitis with choledochal cyst, pancreatobiliary maljunction and ansa pancreatica: A Case Report
Rajat Bansal, Mohammad Irtaza, Anupam Sibal and Hitendra Garg
Indraprastha Apollo Hospital, New Delhi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Recurrent acute pancreatitis (RAP) in children is rare and often poses a diagnostic challenge. We report a pediatric case of RAP with a triple whammy of pancreaticobiliary anomalies: choledochal cyst, pancreaticobiliary maljunction (PBM), and ansa pancreatica.
Case Description: A 11-year-old female presented with acute abdominal pain and a history of recurrent pancreatitis since age 4. Initial MRCP revealed choledochal cyst, cholelithiasis and choledocholithiasis. She had undergone ERCP, CBD clearance, and later, Roux-en-Y hepaticojejunostomy with cholecystectomy. Despite surgery, she experienced recurrent episodes of pancreatitis requiring hospitalizations. This episode of pancreatitis was mild and managed conservatively. MRCP showed dilated remnant CBD with intraluminal defects and peripancreatic inflammation in head of pancreas. ERCP revealed a dilated residual CBD stump with small filling defects and a long common pancreaticobiliary channel, and balloon sweeps retrieved small calculi and sludge. Pancreatic duct cannulation via minor papilla revealed an ansa pancreatica loop. Minor papilla sphincterotomy was done but pancreatic stent placement was not possible. The patient had no further pain episodes on follow-up.
Discussion: In pediatric patients, anatomical anomalies should be considered as a cause of RAP. PBM leads to continuous reflux between pancreatic juice and bile, causing pancreatitis and is often associated with choledochal cyst. Ansa pancreatica, a rare anatomic variant, is characterized by a curved communicating duct between the ventral and dorsal ducts. It has been reported with choledochal cysts and can predispose to pancreatitis due to poor pancreatic duct drainage. Treatment typically involves supportive measures, with ERCP and sphincterotomy considered in refractory cases.
PP-03-011
Insidious colitis: amebiasis
Dmitriy Baranov and Anna Poddymova and Evgeniy Solonitsyn
Almazov Nmrc, Saint-Petersburg, Russia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Currently, inflammatory bowel diseases (IBD) are widespread and well-known, but another pathology may be hiding under this mask.
Materials and methods: We present the case of a white 61-year-old man who, against the background of complete well-being, had an episode of abdominal pain, rectal bleeding, without fever and features of an epidemiological history.
Colonoscopy with colon biopsy was performed. There is ulcerative lesion in the dome of the cecum, EGDS – without features. Laboratory tests for bacterial, viral and parasitic diseases are negative. ANCA, ASCA is the norm. Anemia of mild degree (Gb 122 g/l). Morphology was performed in three different laboratories, only one revealed amoebas. The patient received a course of Metronidazole for 14 days, was sent to an infectious disease specialist: "amoebiasis" was excluded by the doctor. However, inflammatory changes and anemia persisted. On repeated clonoscopies– ulceration is preserved. The diagnosis was made – Crohn's disease of the colon, treatment was started (Prednisone, Azathioprine) – without effect.
A year later, the deterioration of the condition, laboratory parameters. Hospitalization, diagnosis – myeloma. Colonoscopy, morphology: signs of amoebiasis. 5-day course of Paromomycin
Results: At the control colonoscopy: no ulcers, morphology – no amoebas.
The patient is currently being treated for myeloma.
Conclusion: Often a completely different disease is hidden under the mask of one disease, and doctors can make mistakes. In diagnostics, it is important not only to perform analyses, but also to double-check them, despite the opinions of other specialists.
PP-03-012
A successful ERCP outcome in a pregnant patient with acute choledocholithiasis in severe cholangitis
Bautista Carmelo III and Caesar Noel Palaganas and Marie Ellaine Velasquez and Jonathan Viernes
Baguio General Hospital Medical Center, Baguio City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Gallstone-related disease occurs in <1% in pregnancy. This rare non-obstetric emergency may threaten the fetus and mother’s life and requires a multidisciplinary approach. In this report, we aimed to describe clinical and morphological features of acute cholangitis in our pregnant patient and discussed appropriate management.
Case description: This case is a pregnant 28-year-old Filipino female at 29-weeks-age-of-gestation who presented with jaundice and increasing epigastric pain. Ultrasound showed dilated CBD at 1.4cm with a 1.7cm stone in its distal segment. Physical exam showed a gravid, tympanitic and soft abdomen with a positive Murphy’s sign. A diagnosis of obstructive jaundice secondary to choledocholithiasis in severe cholangitis was made. Pertinent laboratories revealed leukocytosis, transaminitis and hyperbilirubinemia. ERCP showed multiple choledocholithiasis and acute purulent cholangitis. Cholangiogram revealed dilated CBD and CHD, with multiple filling defects in the distal CBD, the largest ~18mm. Bile fluid showed growth of Stenotrophomonas maltophilia, TB-PCR was negative. Lastly, a long biliary stent was inserted showing good egress of fluid. No complications were encountered during the procedure.
Discussion: During pregnancy, acute lithiasis cholangitis is rare and present with classic Charcot’s triad. Among imaging studies, abdominal ultrasound remains the first-line examination.Using more specific modalities i.e. CT and MRI may be limited and crucial in pregnancy. Hence, a multidisciplinary approach is necessary. With this, ERCP with sphincterotomy and stone extraction in acute cholangitis among pregnant women is the better and safer therapeutic option. The approach may be challenging for the gastroenterologist but early recognition without delay of complications is necessary.
PP-03-013
Esophageal Stent Insertion: A Bridge to the Treatment of Tuberculous Bronchoesophageal Fistula
Jan Bendric Borbe, Mark Anthony De Lusong and Carlo Jay Dejelo
Philippine General Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Infection and trauma are among the leading benign causes of bronchoesophageal fistulas. Conventionally, bronchoesopahgeal fistulas are treated by surgical resection of the fistulous tract. However, infectious causes, including tuberculosis, have been described in literature to resolve with appropriate anti-microbial treatment. This case describes a patient who underwent esophageal stenting while undergoing anti-Koch’s treatment.
Case Description: The patient is a 37/M, known case of HIV WHO Clinical Stage IV on anti-retroviral agents. He presented with a 1 month history of dysphagia with post-prandial coughing episodes prompting admission. CT scan done on admission showed a bronchoesophageal fistula at the left main bronchus. An upper endoscopy was subsequently performed. An ulcer with a fistulous opening at 31cm from the oral incisors was seen. Segments of the bronchial tree can be visualized at the distal portion of the fistulous tract. Multiple biopsy samples taken over the ulcer showed granulation tissue with marked acute on chronic inflammation. Samples sent for TB GeneXpert came back positive. A fully covered 22x100mm esophageal stent was placed over the fistula. Upon completion of anti-Koch’s treatment, a repeat upper endoscopy was done to remove the esopahgeal stent. The previously noted defect was seen to have re-epithelialized.
Discussion: The case highlights the utility of esophageal stenting in patients with bronchoesophageal fistulas with a tuberculous etiology. It provides an opportunity to allow spontaneous closure of the fistula while the patient is receiving treatment. Furthermore, esophageal stenting provides an enteral access to improve nutritional status and quality of life.
PP-03-014
Treatment of advanced synchronous hepatocellular carcinoma and rectal cancer: A successful case
Quang Loc Bui1, DucHuy Tran2, MauThai Nguyen3, ThangNgoc Nguyen4 and Akiko Tomonari1
1Hanoi French Hospital, Hanoï, Viet Nam;2Bach Mai Hospital, Hanoi, Viet Nam;3VietNam National Cancer Hospital, Hanoï, Vi ệ t Nam;4Central Military Hospital 108, Hanoï, Vi ệ t Nam
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: The frequency of synchronous multiple primary cancers is rare, from 2 to 17% in literature.
Herein, we report the successful treatment of synchronous hepatocellular carcinoma (HCC) and rectal cancer (RC).
Case Report: A 72 years old male, with a history of two small bowel resections for firearm injuries and a cured hepatitis C, presented with multiple HCC’s. The largest lesion was 130x77 mm with portal vein tumor thrombus. AFP was 852.6 ng/ml. Subsequently, an adenocarcinoma was found 5 cm from the anal verge, cT3bN0. CEA was unelevated.
HCC was prioritized. Atezolizumab and bevacizumab (AB) were chosen for the first line treatment. At 4 months after initiating AB, HCC showed partial response with a decrease of the largest lesion to 56x67 mm and dissapearances of the smaller lesions. AFP was decreased to 6 ng/ml.
Following this course, radio-chemotherapy for rectal cancer with CAP-50 protocol was started with concomitant atezolizumab monotherapy. Resection for the rectal cancer was not performed due to the past history of multiple abdominal surgeries. AB was restarted after radiotherapy, which continued to show an excellent response with mRECIST CR for HCC and PR for RC.
Discussion: Synchronous double advanced cancers are rare and can be challenging for prioritizing the target and choosing the optimal agents.
In this case, using bevacizumab concomitant with radiation increased the risk of fistula formation; thus, we decided to use atezolizumab for HCC and CAP-50 protocol for rectal cancer. Conclusively, we were able to achieve sufficient response with no major adverse events.
PP-03-015
A rare case of Dysphagia
Nazish Butt, Ghulam Mohiuddin and Gulzar Khan
Jinnah Postgraduate Medical Centre, Karachi, Pakistan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Esophageal leiomyomas are the most common benign tumors of the esophagus, being pedunculated polyp presentation is very rare. A 65-year-old female having symptoms of troublesome dysphagia was found, incidentally on Esophagogastroduodenoscopy (EGD) as pedunculated polyp of approximately size 3 ×1.7×1.5 cm in size covered by normal looking mucosa. The polypoid lesion was then removed successfully with hot polypectomy snare. The histopathological diagnoses of the polyp turned out as leiomyoma. Patient symptoms were relieved after successful removal.
PP-03-016
A large liver mass turned out to be a rare tumor of Liver
Nazish Butt, Ghulam Mohiuddin and Gulzar Khan
Jinnah Postgraduate Medical Centre, Karachi, Pakistan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Follicular Dendritic Cell sarcoma (FDS) of the liver is a rare entity. It mostly presents in the lymph nodes, extra lymph nodal presentation is very rare A 40 -year-old male having no known comorbidities presented to us with complaints of abdominal pain and abdominal distension for the last 2 months, Triphasic CT scan abdomen showed large irregular mass of approximately size of 17.7 × 15.7×15.9 cm in the right lobe of the Liver with thrombus in the Portal Vein, splenomegaly and mild abdominal ascites representing atypical Hepatoma , the biopsy was done which was consistent with Follicular Dendritic Cell Sarcoma (FDS), a very rare tumor.
Conversion therapy with Durvalumab and GemCis in advanced intrahepatic cholangiocarcinoma achieved an extremely-long recurrence-free period
Yajuan Cao, Jin Peng and Decai Yu
Division of Hepatobiliary and Transplantation Surgery, Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Biliary tract cancer (BTC) is an aggressively invasive malignancy often diagnosed at an advanced stage, precluding surgical intervention and conferring a dire prognosis. The TOPAZ-1 study demonstrated that the combination of durvalumab (D) plus gemcitabine and cisplatin (GC) yielded significant survival benefits. We present a case of stage IV intrahepatic cholangiocarcinoma (ICC) that underwent successful conversion with D plus GC, achieving a recurrence-free status 19 months post-surgery.
Case Description: A 51-year-old male was diagnosed with stage IV ICC, with left supraclavicular lymph nodes metastasis. Following four cycles of GC and two doses of durvalumab, the target lesions shrank by 30% (RECIST 1.1), with the disappearance of distant lymph nodes and a notable reduction in regional lymph nodes, achieving partial remission (PR). The patient was subsequently downstaged and underwent laparoscopic radical liver resection and regional lymph nodes dissection. Postoperative pathology revealed ICC with tumor necrosis rate of approximately 90%. Adjuvant therapy with durvalumab was administered for six months post-surgery. The patient has remained disease-free for over 19 months.
Discussion: Radical surgery remains the cornerstone of treatment for BTC, offering the best chance for long-term survival. The patient had high expression of PD-L1. The addition of PD-L1 inhibitor to the previous standard treatment (GC) had significant therapeutic effects and provided the patient with the opportunity for radical surgery. Continuing adjuvant therapy post-surgery has prolonged his disease-free interval to 19 months. The D+GC regimen presents a promising alternative for conversion therapy in advanced BTC patients, underscoring further exploration in extensive clinical trials.
PP-03-018
Eosinophilic gastrointestinal disease involving multiple segments of the gi tract with ascites: a case report
Jobel Feliz Castillo-Corañez and Eulenia Nolasco
Manila Doctors Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Eosinophilic gastrointestinal disease (EGID) is a rare inflammatory condition characterized by eosinophilic infiltration of specific segments of the gastrointestinal tract, leading to diverse symptoms. This case report discusses a 53-year-old female with an uncommon EGID presentation involving multiple segments from both the upper and lower GI tract. She also presented with ascites, another rare manifestation.
Case description: The patient presented with reflux, heartburn, bloatedness, epigastric pain, vomiting, and loose watery stools. Symptoms persisted despite initial treatment for GERD and infectious diarrhea. Investigations revealed significant eosinophilia in peripheral blood and was also noted to have eosinophilic ascites. Endoscopy and colonoscopy were done, with multiple biopsies, and confirmed eosinophilic infiltration in the esophagus, stomach, small intestine, and colon. The patient was treated with montelukast and corticosteroids, which resulted in rapid and marked improvement of all her symptoms.
Discussion: EGID’s clinical manifestation is dictated by the specific segment (esophagus, stomach, intestine, or colon) and the layer of the GI wall (mucosa, muscularis, serosa) involved. This case underscores the rare and complex presentation of EGID in our patient involving multiple GI segments consistent with her symptoms. Likewise, the presence of ascites suggests deep eosinophilic infiltration to the serosal layer, which is the least common layer affected. This case contributes knowledge to the literature that despite multiple segments and bowel wall layer involvement, the condition is very responsive to therapy, emphasizing the importance of awareness of this condition among clinicians for early diagnosis and appropriate treatment.
PP-03-019
An Extremely Rare Case of Secondary Aorto-enteric Fistula in a Filipino Patient with Herald Bleed
Alyssa Chan
St Luke's Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Secondary aortoenteric fistula (SAEF) is a fistulous communication between the duodenum and the aorta. SAEF is a rare yet lethal complication of abdominal aortic reconstruction which arises months to years after aortic surgery. Herald bleeding refers to a bleeding episode with abdominal pain that occurs before a severe hemorrhage. This is a case of SAEF in a young male who presented with massive hematochezia.
Clinical Presentation: 32-year-old male with past medical history of abdominal aortic aneurysm repair presented with herald bleeding of 3 weeks’ duration. Endoscopy showed unremarkable results. Examination revealed tachycardia, generalized pallor and epigastric tenderness. Tests showed hemoglobin of 7.9 mg/dL, platelets of 251,000 /μL, and INR of 1.26.
Results: Tagged RBC scan was negative. Antegrade Push Enteroscopy showed an SAEF with eroding graft at segment D3-D4. Exploratory laparotomy revealed the secondary aortoduodenal fistula at D3 segment with multiple pockets of necrotic tissue and densely adherent to the native aorta and graft. The patient underwent SAEF repair and post-operative course was complicated by sepsis and DIC and eventually went into arrest on his fourth week.
Significance: This is the first case of SAEF documented at our institution, and possibly within the nation as well. It is extremely rare with an estimated annual incidence of 0.036–1.6%.
Recommendations: Every patient presenting with UGIB with a history of aortic graft surgery should be presumed to have an aortoenteric fistula until proven otherwise. All patients should undergo early endoscopy as prompt surgical intervention is the key to survival among these patients.
PP-03-020
Case Report: AI-Assisted Capsule Endoscopy Rapidly Detecting Obscure Gastrointestinal Bleeding in High-Risk Cardiac Patients
Wah Loong Chan, Sivaraj Xaviar, Esther Jara Edmund Nyipa and Stanley Khoo
University Of Malaya, Kuala Lumpur, Malaysia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: This report details our experience using Artificial Intelligence (AI)-assisted capsule endoscopy in diagnosing obscure gastrointestinal bleeding (GIB) in a high-risk patient with a recent non-ST elevation myocardial infarction (NSTEMI).
Case description: A 79-year-old man with coronary artery disease presented with NSTEMI and underwent percutaneous coronary intervention (PCI). He was initiated on antiplatelet therapy but he developed melena and his haemoglobin dropped until 6.4g/dL. Conventional diagnostic methods, including upper endoscopy, colonoscopy, and CT angiography, failed to locate the bleeding source. His hemoglobin continued to decline by 1 g/dL daily despite transfusions. We employed AI-assisted capsule endoscopy (NaviCam® SB System with ProScan™ AI) to identify the cause of his persistent anemia.
Initial colonoscopy revealed clots and blood suggesting small bowel bleeding, but further examinations, including a second colonoscopy, showed no active bleeding. AI-assisted capsule endoscopy quickly detected mid-jejunal bleeding within minutes upon initiation of its ProScan™ AI feature, displaying a much faster analysis than the manual review methods. This led to surgical intervention, where an exploratory laparotomy and on-table enteroscopy confirmed an arteriovenous malformation 70 cm beyond the duodenojejunal flexure, which was successfully resected.
Discussion: Our experience highlights the transformative potential of AI-assisted capsule endoscopy in managing obscure GIB. The technology’s quick and precise detection was crucial for timely surgical intervention, enabling prompt management and continuation of essential antiplatelet therapy in this high-risk patient.
PP-03-021
A case of ulcerative colitis associated with Hypersensitivity Ectodermal Dysplasia
Tsubomi Cho and Shingo Tsuda
Tokai University, Hachioji, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Ectodermal dysplasia is a genetic disorder that causes dysplasia of tissues derived from the ectoderm, such as skin appendages, teeth, and nails. Among them, anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) is caused by mutations in the gene encoding nuclear factor-κB essential modulator protein (NEMO) and abnormalities of the inhibitor of NFκB α (IKBA) gene have been reported.
Case report: The patient reported here is an 18-year-old man with an EDA-ID–like phenotype, including ectodermal dysplasia with sparse hair (hypotrichosis), abnormal conical teeth, and hypo hidrosis. He had recurrent hemorrhoids fistula since childhood. In 20XX, he visited hospital due to fever, diarrhea, and bloody stools. Colonoscopy was performed and referred to our department because ulcerative colitis was suspected. In colonoscopy, revealed edema, redness, inflammation and pus are appearance in the sigmoid colon and rectum. Pathology showed diffuse inflammatory cell infiltration, crypt abscess, goblet cell depletion, and abnormal glandular arrangement. The diagnosis of ulcerative colitis was made.
Upper gastrointestinal endoscopy and small bowel angiography were performed and no abnormalities were found. He was treated with mesalazine and prednisolone which provided symptom improvements and discharged in Day28.
Discussion: In this case, his father and aunt had the same disease, so NEMO gene abnormality (X-linked recessive heredity) is assumed to be present. This type of EDA-ID is estimated to be 1 in 250,000 individuals. Patients who have NEMO gene abnormality with ulcerative colitis is rare condition.
PP-03-022
Gastrointestinal Tuberculosis Appearing as a Colon Mass
Franz Jeryl Liangco, Nicole Allyson Chua, Gina Tecson and Kenji Sato
Cardinal Santos Medical Center, San Juan City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal tuberculosis (GI TB) accounts for approximately 1% to 3% of all the tuberculosis cases worldwide. Early diagnosis remains difficult, due to the nonspecific clinical presentation of TB, which can mimic other gastrointestinal diseases and may vary from an acute to a chronic abdomen in areas endemic for TB. A delay in diagnosis and treatment can result in significant morbidity and mortality.
Case Description: This is a case of a Filipino male in his early 50s, with no known comorbidities, with 1-year history of 11-kg weight loss, and a strong family history of colon cancer, who underwent screening colonoscopy revealing an ulcerating, friable, granular mass at the ascending colon approximately 1-1.5cm in widest diameter. Magnified chromoendoscopy suggests non-neoplastic features. Multiple biopsies taken and showed negative TBGeneXpert as well as absence of acid-fast bacilli but showed chronic granulomatous inflammation with multinucleated Langhans type giant cells as well as acute-on-chronic colitis with mucosal erosion and architectural distortion; positive for crypt abscess. Patient was then started on empiric anti-tuberculosis treatment. The patient completed a 6-month course of the anti-tuberculosis regimen with repeat colonoscopy revealing mucosal healing of the intestinal lesion, as well as weight gain of 2-kg post-treatment.
Discussion: Gastrointestinal tuberculosis poses a diagnostic challenge as it typically presents with non-specific clinical, radiologic and endoscopic features. High index of clinical suspicion and appropriate use of investigative modalities, can aid in early recognition, diagnosis and prompt treatment thereby reducing morbidity and mortality.
PP-03-023
Gastrointestinal Tuberculosis Mimicking Ulcerative Colitis in a Young Filipino Female
Vernon Chuabio
Philippine General Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal tuberculosis (TB) can mimic numerous conditions like inflammatory bowel disease (IBD). A usual dilemma is differentiating between gastrointestinal TB and Crohn disease since both commonly involve the ileocecal region, unlike ulcerative colitis (UC). This case describes a patient initially assessed to have UC but eventually diagnosed with gastrointestinal TB.
Case Description: A 23-year-old Filipino female with no known comorbidities and unremarkable family history presented with 1-month watery diarrhea, rectal pain, and weight loss. She had no fever, abdominal pain, or hematochezia. On physical examination, she had bitemporal wasting, pink palpebral conjunctivae, and soft nontender abdomen. A firm, blood-tinged, nodular mass was noted on digital rectal examination. Contrast-enhanced abdominal CT scan showed a 6 x 6.2-cm circumferential, enhancing rectal mass. Colonoscopy revealed a 10 cm-long friable mass-like ulcerating lesion in the rectum, involving the anal verge. Transversely oriented clean-based ulcers 3-4 cm-long were seen in the right colonic side and sigmoid. The remaining colonic segments and terminal ileum were normal-looking, while the ileocecal valve was competent and also normal-looking. Mesalazine was started given an overall presumptive diagnosis of UC. Biopsies finally showed chronic granulomatous inflammation with caseation necrosis. Sputum tests also microbiologically confirmed TB. With the revised TB diagnosis, mesalazine was shifted to anti-tuberculosis therapy.
Discussion: A lack of ileocecal involvement does not rule out gastrointestinal TB, which may still be considered in left-sided colonic disease that can mimic UC, especially in TB-endemic regions. Correlation between clinical, endoscopic, and histologic findings is crucial for proper diagnosis and treatment.
PP-03-024
Varied Presentations of Adult Gastrointestinal Tuberculosis: A Case-series Of 5 Filipino Patients
John Derek Clutario
Makati Medical Center, Pasig, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Tuberculosis (TB) showed an increasing rate from 2020 to 2022. Among the cases of TB, 15-25% was estimated to be extrapulmonary. Among these, gastrointestinal tuberculosis, the most common form of abdominal TB, has shown a rising trend in recent years. It poses significant diagnostic and therapeutic challenges due to the non-specific and varied nature of its symptoms, limited diagnostic test accuracy, impaired drug absorption with GI involvement, and drug resistance. Timely diagnosis and treatment are essential to prevent severe morbidity, prolonged hospitalization, and the need for surgical intervention.
Case description: This is a case series of 5 patients showing the wide array of presentations of GI TB like severe abdominal pain, dysphagia, gastrointestinal bleeding, gastric outlet obstruction and even a palpable mass.
Case 1: 44-year-old female with SLE on immunosuppressants presented with dysphagia and gastrointestinal bleeding. Treatment with an anti-TB regimen and endoscopic hemostasis controlled the bleeding. Case 2: Initially presented with symptoms of gastric outlet obstruction and vomiting. Case 3: A 20-year-old female presented with severe abdominal pain. Case 4: A 24-year-old female presented with right upper quadrant pain and a palpable mass. Case 5: 60-year-old male with a history of pulmonary TB presented with severe abdominal pain and partial bowel obstruction.
Discussion: This study describes the epidemiology, risk factors, and current diagnostic and treatment strategies of GI TB in the given cases. This case series underscores the diverse clinical manifestations of gastrointestinal TB and highlights the need for heightened awareness and timely intervention to improve patient outcomes.
PP-03-025
Non-cirrhotic portal hypertension from a portal vein fusiform aneurysm with arteriovenous malformation: A Case Report
John Derek Clutario
Makati Medical Center, Pasig, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Disorders associated with noncirrhotic portal hypertension are categorized based in the site of involevement. It can be prehepatic, intrahepatic or posthepatic. Our case involves both a prehepatic and intrahepatic cause of noncirrhotic portal hypertension.
Case description: A 16 year-old male presented with severe right lower quadrant and hematochezia managed as a case of Ruptured diverticulosis and underwent Diagnostic Laparoscopy, Peritoneal Lavage, Drainage of Pelvic Abscess. He was readmitted for left lower quadrant pain now associated with loose watery stools. This event was assessed as another episode of ruptured diverticulitis. Five weeks later, he was readmitted for loss of consciousness and anemia. Endoscopy revealed esophageal varices. Rubber band ligation was done which resolved the anemia and was discharged stable. Three months after discharge, he had another episode of loss of consciousness and recurrence of anemia. Imaging revealed portal hypertension associated with a large aneurysm involving the confluence of the common hepatic artery with fistula formation to the right portal vein. He underwent another endoscopy still revealing esophageal varices where rubber band ligation was again done. The patient was stable and advised to undergo whole exome sequencing to assess for a possible connective tissue disorder particularly vascular Ehler-Danlos syndrome.
Discussion: We have a patient presenting with abdominal pain, loss of consciousness and anemia. The search for the cause of anemia revealed esophageal varices leading to further imaging showing portal hypertension from a common hepatic artery aneurysm and arteriovenous malformation.
PP-03-026
Overt gastrointestinal bleeding from a pseudoaneurysm complicating a case of necrotizing pancreatitis: A case report
John Derek Clutario
Makati Medical Center, Pasig, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Acute pancreatitis is a common disease with multiple known risk factors and triggers. Mild cases have good prognosis however when severe, could lead to several complications. This case report discusses severe pancreatitis with several complications including the fistula formation and pseudoaneurysm.
Case description & discussion: The patient is a 59 year-old male initially managaed as a case of gallstone pancreatitis and underwent subsequent cholecystectomy. Two days after discharge, he was readmitted for recurrence of epigastric pain now managed as acute pancreatitis with peripancreatic fluid collection with some necrotic component. He was medically managed and was discharged stable. Five days after, he had isolated back pain, chills and vomiting episodes. CT revealed acute pancreatitis on top of necrotizing pancreatitis with hemorrhagic component which was initially managed with antibiotics. Repeat scans revealed progression of acute hemorrhagic pancreatitis to necrotizing pancreatitis. Due to the multiloculated nature of the fluid collection, drainage was held off and medical management was maximized where the patient recovered. Two weeks after he was readmitted for hematochezia. An endoscopy was done which revealed an ulcerating friable mass with an oozing duodenal bleed where argon plasma coagulation was done. Post procedure the patient continued to be unstable hence a mesenteric angiogram was done which revealed a pseudoaneurysm and subsequent embolization was done. Clinical status was stable and repeat endoscopy with endoscopic ultrasound was done revealing a fistulous opening oozing with purulent discharge. Medical management was maximized and a nasoduodenal tube was inserted to bypass the lesions and D2 segments.
PP-03-027
TWIST OF FATE: Small Bowel Intussusception secondary to Peutz-Jegher Syndrome
Rial Juben De Leon, Christine Marianne Sy, Ma Teresita Gamutan and Esparanza Grace Santi
De La Salle University Medical Center, Dasmarinas, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Acute intestinal intussusception in adults is rare, accounting for only 5% of all cases of intussusception and contributing to 1-5% of intestinal obstructions.
This study reports a case of a 33-year-old Filipino male without comorbidities who presented with sudden, severe abdominal pain, post-prandial vomiting, hematochezia, and obstipation. His family history was notable for hamartomatous polyps in his father and sibling. PE revealed hyperpigmented macules on lips and feet, hypoactive bowel sounds, and rebound tenderness in the upper abdomen.
Plain abdominal CT scan revealed small bowel intussusception. Laboratory findings were unremarkable except for mild anemia and leukocytosis. Emergency laparotomy confirmed the jejuno-jejunal intussusception and revealed palpable lesions in the colon, prompting referral to gastroenterology. Colonoscopy uncovered multiple varied sized polyps with grossly dysplastic features in the ascending and sigmoid colon. Histopathology of excised intestinal segments and colonic polyps revealed hamartomatous polyps establishing the diagnosis of Peutz-Jegher Syndrome (PJS). Post-operatively, the patient showed improvement and was discharged after a 10-day hospital stay.
PJS is characterized by progressive polyp growth, leading to complications such as pain from polyp infarction, chronic bleeding, and intestinal obstruction. It is also associated with a significantly increased risk of both gastrointestinal and extra-intestinal cancers. Treatment primarily involves surveillance and symptom management, with surgical intervention necessary for complications like intussusception. Regular surveillance every 2-3 years is recommended post-diagnosis to mitigate risks associated with PJS, including bleeding, obstruction, and cancer development. While no cure exists for PJS, early detection and intervention can significantly improve patient outcomes and quality of life.
PP-03-028
Unveiling the enigma: banti‘s syndrome and the mysteries of congestive splenomegaly
Rial Juben De Leon and Rona Marie Lawenko
De La Salle University Medical Center, Dasmarinas, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
An 18-year-old, non-alcoholic, Filipino female was hospitalized following an acute episode of hematemesis and melena. No history of prior NSAID, steroids, or herbal use. With no significant prior medical conditions, the patient had pallor with signs of hypovolemia. There was no jaundice, skin lesions, abdominal pain, edema, altered sensorium, or fever. Abdominal PE was unremarkable. Severe anemia (54 g/L) and hypoalbuminemia (15 g/L) were noted. Transaminases and other liver function tests were normal. APRI score was 0.5. PBS was negative for atypical cells or malarial parasites. Viral hepatitis panel and ANA were negative. Ultrasound revealed normal-sized liver, mild splenomegaly with no signs of cirrhosis, mass, or ascites. The patient was initially started on Omeprazole and Octreotide drip and IV Ceftriaxone. Blood transfusion was done. Subsequent esophagogastroduodenoscopy revealed grade 3 esophageal varices with mild portal hypertensive gastropathy. No gastric/duodenal varices seen. Rubber band ligation was done and later on, she was started on Propanolol for secondary prophylaxis. She was eventually discharged improved.
The diagnosis of Banti's syndrome was established which is characterized by persistent splenic enlargement leading to blood cell destruction with subsequent anemia, and signs of portal hypertension without any liver pathology. While the exact pathophysiology remains unclear, the syndrome typically presents with gastrointestinal hemorrhage and splenomegaly resulting to pancytopenia. In emergency situations, endoscopic sclerotherapy and/or variceal band ligation can be performed, and beta-blockers are recommended for primary prophylaxis in portal hypertension, irrespective of cirrhosis, while splenectomy may be necessary for recurrent bleeding and severe anemia requiring multiple transfusions.
PP-03-029
Empyema Thoracis from Transdiaphragmatic Extension of Pyogenic Hepatic Abscess, A Case Report
Christopher Delima, Lady Angela Robles and Melissa Estur
Divine Word Hospital, Tacloban City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Empyema thoracis as a complication of pyogenic hepatic abscess is a rare condition yet lethal. It is unusual with an incidence rate ranging from 0.5% to 0.8%.and recent literatures are limited to only 23 cases.
Case Report: A 53-year-old female experienced right upper quadrant abdominal pain and fever two weeks prior to admission. It was associated with dyspnea. Patient is newly diagnosed with Type 2 Diabetes Mellitus. Whole abdominal computed tomography scan with contrast revealed irregular thick walled multiloculated lesion in the right hepatic lobe considering hepatic abscess with signs of capsular and diaphragmatic rupture extending to right pleural space. Patient undergone emergency exploratory laparotomy, evacuation of hepatic abscess, diaphragm repair, and closed tube thoracostomy, right. Blood and abscess cultures noted no growth. Patient observed to be clinically improving. Antibiotic therapy completed. Closed tube thoracostomy removed and discharged.
Discussion: Pyogenic hepatic abscess is both a diagnostic and therapeutic challenge as clinical signs are nonspecific despite modern imaging techniques. Type 2 diabetes mellitus remains a strong risk factor for its development. Drainage of both the hepatic abscess and empyema with antibiotic therapy is recommended to achieve good prognosis as drainage of only one site will not be adequate for resolution of the disease Multidisciplinary management and early detection are essential to achieve good outcomes as evidence is still scarce. This case requires standard clinical guidelines and further investigation.
PP-03-030
Resolving Buried Bumper Syndrome with Balloon-Assisted Percutaneous Endoscopic Gastrostomy Placement: A Case Report
Ma. Regina Dimaculangan, Laurence Laurel, IV Federico Peralta and Alexandra Laya
St. Luke's Medical Center - Global City, Taguig, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Buried bumper syndrome is a serious complication associated with long-term PEG tube placement, and management often involves endoscopic interventions. These procedures can be challenging in certain patients, hence the Balloon-assisted PEG placement (BAG) emerged as a promising option. This technique involves the use of an inflatable balloon catheter to help dilate the gastrostomy site, facilitating easier passage of the new PEG tube, and potentially reducing complications.
Case Description: A 91 year old male with known chronic cerebrovascular disease was admitted as a case of Buried Bumper Syndrome. The PEG tube - Mushroom type, was fixed, anchored, and cannot be pushed into the stomach lumen, confirming the CT Scan findings of the PEG tube located within the anterior abdominal wall. Since the patient was unstable to undergo EGD guided PEG replacement, the team decided to try a bedside approach. A guidewire was first placed coursing through the existing PEG site. A catheter was advanced over the wire, and water soluble contrast was flushed. Spot x-ray image revealed contrast in the small bowel. The catheter was then removed, and a CRE Balloon Dilator was advanced over the wire, and inflated sequentially from 8-10mmHg to achieve the desired tract dilation. A F24 Balloon PEG Tube was then advanced and secured in place. Contrast was again flushed, and radiographs confirm its intra-gastric placement. Patient tolerated the procedure well with no untoward events.
PP-03-031
Ileo-colic Intussusception secondary to Primary Diffuse Large B-cell Lymphoma of the Cecum: A Case Report
Ma. Althea Kathrine Elinzano
East Avenue Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Ileo-colic intussusception refers to the invagination of the ileum into an adjoining segment of the ascending colon. Intussusception in adults is rare and is often associated with malignancy. Primary colorectal lymphoma is a rare phenomenon among malignancies affecting the colon.
Case report: We present a case of a 67-year-old Filipino male with one month history of vague abdominal pain and unintentional weight loss. The medical history was unremarkable. He was advised colonoscopy wherein a bulky, partially obstructing, nodular mass at the cecum was noted. Histopathology and immunohistochemical staining revealed lymphoma of the diffuse large B-cell type. Contrast CT scan of the abdomen showed a segment of the terminal ileum pulled into the ascending colon. Right hemicolectomy was done. There was a 6.5x6.0x4.0 cm tan brown, doughy mass at the cecal segment, completely obstructing the lumen. Surgical margins were negative for tumor invasion, however, there was lymph node involvement. The patient was advised for chemotherapy.
Discussion: Primary cecal lymphoma presenting with intussusception is extremely rare and requires a multi-disciplinary approach. Surgical intervention is recommended without attempting prior reduction, given the likelihood of encountering malignancy and the associated risks of perforation and tumor spread.
PP-03-032
Autoimmune Hepatitis and Systemic Lupus Erythematosus Overlap Syndrome: A Case Report
Ma. Althea Kathrine Elinzano
East Avenue Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Autoimmune hepatitis (AIH) and systemic lupus erythematosus (SLE) are distinct autoimmune disorders characterized by aberrant immune responses targeting various organs and tissues. However, overlap between these conditions, poses diagnostic and management challenges due to its rarity and heterogeneous clinical presentation.
Case report: We present a case of a 40-year-old female with a 5-year history of joint pains, malar rash, undocumented fever, and jaundice. Initial laboratory tests showed elevated liver enzymes. ANA was positive, with a titer of 640, and serum IgG was elevated. She underwent liver biopsy and was compatible with autoimmune hepatitis. She was maintained on prednisolone, however, due to resolution of symptoms, the patient was lost to follow-up and discontinued her medications for two years. Subsequently, she developed recurrence of fever, jaundice, joint pains, with associated increasing trends of liver enzymes. She was diagnosed to have a relapse of autoimmune hepatitis type I, with systemic lupus erythematosus. Contrast CT scan of the abdomen and Magnetic Resonance Cholangiopancreatography (MRCP) revealed an unremarkable liver and biliary tree. Serum ammonia, liver enzymes, bilirubins, and INR were elevated. The patient underwent Double Plasma Molecular Adsorption System (DPMAS) for impending liver failure. Methylprednisolone pulse therapy was initiated and was subsequently tapered to prednisone. Clinical and biochemical improvement was noted prior to discharge from the intensive care unit. Normalization of liver enzymes and resolution of jaundice was noted on follow-up.
Discussion: This case underscores the importance of multidisciplinary collaboration and individualized care in managing this complex autoimmune syndrome.
PP-03-033
Sinistral Portal Hypertension Secondary to Chronic Pancreatitis in a 20-year old Male: A Case Report
Ma Althea Kathrine Elinzano
East Avenue Medical Center, Quezon City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Sinistral portal hypertension is a rare condition characterized by the obstruction or thrombosis of the splenic vein, leading to increased pressure in the splenic circulation. Chronic pancreatitis (CP) is a progressive inflammatory disease of the pancreas characterized by irreversible damage to pancreatic tissue, leading to persistent abdominal pain, malabsorption, and impaired quality of life.
Case report: We present a case of a 20-year-old male with a 4-year history of recurrent epigastric pain radiating to the back. He had prior multiple admissions for recurrent necrotizing pancreatitis. Contrast CT scan of the abdomen showed severe necrotizing pancreatitis, splenomegaly, and multiple dilated perisplenic and gastric collateral vessels. Splenic doppler ultrasound revealed splenic vein thrombosis. An isolated gastric varix at the cardia was seen in esophagogastroduodenoscopy. Endoscopic ultrasound showed a diffusely hypoechoic pancreatic body, with stranding, hyperechoic foci and lobulations at the pancreatic head, consistent with chronic pancreatitis. The patient was maintained on propranolol and apixaban. Hematologic work-up for JAK2 mutation and myeloproliferative disorders were unremarkable. ANA and IgG4 were within normal. The patient was advised further genetic testing for a hereditary etiology of chronic pancreatitis.
Discussion: Although rare, understanding the less common complications of chronic pancreatitis is crucial for clinicians to effectively diagnose and manage patients with this condition, ultimately improving patient outcomes and quality of life.
PP-03-034
Adhesion-induced gastric stenosis four years after adjustable gastric band removal surgery
Sang Soo Eom and Seok In Kang
Inje University College Of Medicine, Ilsan Paik Hospital, Gyunggi, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Laparoscopic adjustable gastric band surgery (LAGB) was previously performed as a treatment for obesity, but recently, due to various complications, it is gradually being ruled out. Although various complications have been reported after LAGB such as band erosion, reports of gastric stenosis after gastric band removal surgery are extremely rare. Herein, we report a rare case of adhesion-induced gastric stenosis 4 years after adjustable gastric band removal.
Case Description: A 60-year-old female presented to the emergency department with recurrent vomiting. She had undergone LAGB 6 years prior, and the adjustable gastric band was removed 4 years ago. The laboratory results showed elevated serum creatinine (2.92 mg/dL), indicating acute kidney injury, and hypokalemia (2.6 mmol/L). Gastric stenosis was diagnosed by pre-operative computed tomography (CT) and esophagogastroduodenoscopy (EGD) (Figure 1). Laparoscopic surgery was performed to resolve stenosis, severe fibrosis encircling mid body of the stomach was in the surgical field; lymph node station 4sb and 7 were adhered to the anterior wall and posterior wall of the stomach, respectively, due to adhesion (Figure 2). Subsequently, adhesiolysis was performed, resolving gastric deformity. Post-operative CT and EGD confirmed the restoration of the stomach’s normal anatomical structure (Figure 3).
Discussion: Despite its rarity, adhesion-induced gastric stenosis should be considered as a differential diagnosis even after removal of adjustable gastric band, necessitating adhesiolysis.
PP-03-035
Budesonide for Immune Checkpoint Inhibitor-associated Gastritis: A Case Series
Scott Mackay1, Stephen Wolman2 and Flavio Habal3
1Toronto General Hospital UHN, Toronto, Canada;2University of Alberta, Edmonton, Canada;3Toronto General Hospital UHN, Toronto, Canada
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Immune checkpoint inhibitor (ICI) therapies are used to treat a variety of malignancies and have well-established adverse effects throughout the gastrointestinal tract.1 “ICI-associated gastritis” is a less recognized complication of ICI therapy which can lead to significant complications of inflammation and bleeding and intolerance to oral intake.2 Literature to date reports attempts at treatment of “ICI-gastritis” with proton pump inhibitors, gastrointestinal protectants, and systemic glucocorticoids (i.e., prednisone, methylprednisolone), which are often not successful. There remains no specific guidelines for management.3 This case series summarizes four patient cases in which budesonide (Entocort®) led to dramatic symptomatic clinical resolution.
Case Series: Four patients receiving ICI therapy developed ICI-associated dyspeptic symptoms. Their symptoms included nausea, vomiting, anorexia, weight loss, and epigastric discomfort with onset ranging from one cycle to four years after initiation of ICI therapy. Endoscopic findings were varied with having normal appearing mucosa or erythema and erosions. Pathology consistently showed increased intraepithelial lymphocytes and varying severities of inflammation. All patients reported symptomatic improvement within two to four days of starting budesonide 9 mg oral daily. Endoscopic appearance was unchanged.
PP-03-036
Unique chlorophyllum molybdites poisoning : role of polymeric protein in molybdophyllysin
Angkasa Hamdan and Dolvy Girawan and Muhammad Begawan Bestari and Nenny Agustanti and Palar Wijaya
Universitas Padjadjaran, Kota Bandung, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract
Background: Since time immemorial, identifying edible mushroom for consumption becomes the main problem of mushroom poisoning, even for experienced person. Mushroom toxin consist of protoplasmic, neurotoxin, and gastrointestinal irritation. However, Chlorophyllum molibdites sp. poisoning can manifest in peculiar fashion due to its unique properties of protein.
Case Report: There were three cases presented, who suffered from various clinical manifestations after mushroom consumption during camping at the same time. A 36 years old male experienced altered consciousness and elevated level of transaminase (SGOT/SGPT: 1804/4139). Unusual ascites was found. There was no decline in renal function despite indication of acute liver failure. A 20 years old male experienced elevated level of transaminase (SGOT/SGPT: 8233/7521), jaundice, hypoalbumin and unusual ascites without altered consciousness. Lastly, a 26 year old male experienced severe diarrhea with moderate elevation of transaminase (SGOT/SGPT: 2108/2632).
Discussion: Chlorophyllum molybdites causes most of mushroom poisoning in Indonesia. Polymeric protein of Chlorophyllum sp consist of complex arrangements which can be influenced by even demographic area and climate its found. We identified a patient with severe liver failure who didn’t manifested any decline in his kidney function, in contrast to most severe mushroom poisoning.
Department of gastroenterology and hepatology, Tokyo medical university, Shinjyuku-ku, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: In symptomatic pancreatolithiasis, treatment options include Extracorporeal Shock Wave Lithotripsy (ESWL) and Endoscopic Retrograde Cholangiopancreatography (ERCP). The effectiveness of pancreatic stone fragmentation through laser lithotripsy (LL) under peroral pancreatoscope (POPS) has been reported. We present a case where pancreatic stone removal was performed using a novel slim POPS following LL.
Case Description: An 80-year-old male with chronic pancreatitis experienced recurrent acute pancreatitis due to a 10mm pancreatic stone detected via endoscopic ultrasound in April 20XX. In May, ERCP revealed stones in the pancreas head. A drill dilator was used to penetrate the stones and advance into the pancreatic duct distal to the stones, where the POPS was then inserted. The stones were fragmented using a Yttrium aluminum garnet (YAG) laser. Due to removal difficulties, a pancreatic stent was placed. Subsequent ESWL and YAG laser treatments fragmented residual stones. However, removing these smaller fragments under fluoroscopy was challenging; thus, under POPS guidance, a retrieval basket was deployed distal to the stones, allowing for the extraction of these smaller fragments into the intestinal tract along with the POPS.
Discussion: The use of POPS facilitates reliable stone removal and prevents basket impaction. The novel slim design allows treatment in cases with narrow pancreatic ducts, ensuring safe and definitive stone removal.
PP-03-038
A case of local recurrence of the cholangiocarcinoma 10 years after surgery
Kento Hisamatsu, Kotaro Takeshita, Yuma Fujita and Satoshi Asai
Tane General Hospital, Osaka, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: In the gastrointestinal malignancies, postoperative follow-up is commonly completed after 5 years without recurrence. we experienced a case of distal cholangiocarcinoma that recurred 10 years after surgery.
Case Description: The patient is a 67-year-old Japanese male. He underwent pancreatoduodenectomy and Billroth-II reconstruction for distal cholangiocarcinoma. The chemoradiotherapy, the follow-up was completed without recurrence at 5 years after surgery. 5 years later, he came to our hospital with a fever. CT scan showed a liver abscess and antibiotics was started. However, the contrast-enhanced CT scan showed an enlarged abscess, so percutaneous transhepatic abscess drainage (PTAD) was performed on the 14th day. Although the abscess was almost improved, contrast through the PTAD showed the abscess and posterior branch of the bile ducts were in communication and the bile drainage of the PTAD did not decrease, ERCP was performed on the 18th day. There was a stenosis at the bile duct jejunal anastomosis, which was suspected to be a recurrence. Cholangiography showed bile duct stenosis at various points in the intrahepatic ducts. Since stent placement in the posterior bile duct was a failure due to severe stenosis, stenting was endoscopically completed assisted by a PTAD route the other day. A biopsy of the stenosis at the anastomosis revealed adenocarcinoma pathologically similar to the cholangiocarcinoma of 10 years ago.
Discussion: We report here a rare case of local recurrence of distal cholangiocarcinoma 10 years after surgery.
PP-03-039
Linitis Plastica in a patient with breast cancer: primary gastric cancer or breast cancer metastases?
Jonas Ho
Singapore General Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Linitis plastica is a challenging diagnosis in the setting of a patient with previous breast cancer.
Case Description: A 74-year-old Chinese Female with a history of left breast invasive ductal cancer (ER+, PR+, HER+) 4 years prior, and completed neoadjuvant surgery and chemotherapy, with clinical remission on letrozole.
She had presented with epigastric pain, nausea, early satiety and weight loss of 5kg and an Oesophageogastroduodenoscopy (OGD) which revealed that gastric folds were thickened and prominent with overlying pangastritis associated with mucosal oedema and sloughing.
Gastric biopsies done demonstrated gastric mucosa with features of poorly differentiated adenocarcinoma with signet ring cells. Immunohistochemistry demonstrated CDX2 positivity in tumour cells. CK7 and CD20 show variable positivity in tumor cells. Staining for GATA3 and ER were performed which was negative. TTF-1 was also negative. The degree of HER2 overexpression was equivocal. The overall findings are conclusive for a primary gastric adenocarcinoma.
A computer tomography of the thorax and abdomen was performed which showed diffuse mural thickening around the distal oesophagus and stomach with enlarged perigastric lymph nodes. She subsequently underwent a diagnostic laparoscopy revealing peritoneal metastases, and was started on systemic chemotherapy.
Discussion: Differentiating primary gastric adenocarcinoma and metastatic breast cancer is of pivotal importance given the downstream implications for treatment and prognosis. Immunohistochemistry remains the definitive means of ascertaining this where positivity for GATA3 and ER and CK7 is consistent metastatic breast cancer.
Therefore, deep biopsies or even endoscopic ultrasound guided needle biopsies are warranted when the index of suspicion is high.
PP-03-040
Amebic Colitis presenting with Chronic Iron-Deficiency Anaemia
Naung Latt Htun and Dilip Thottacherry
Suri Seri Begawan Hospital, Ministry Of Health, Kuala Belait, Brunei Darussalam
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Entamoeba histolytica is an intestinal protozoan that causes a wide range of intestinal problems. It is common in many parts of the world and is transmitted by faecal-oral route. It can cause chronic iron-deficiency anaemia due to bleeding from an amoebic ulcer or colitis.
Case Description: A thirty-year-old man presented with dizziness and fatigue. The examination was unremarkable, apart from mild conjunctival pallor. The blood tests showed haemoglobin of 10.7 with MCV 87 and transferrin saturation of 5. He underwent OGD and colonoscopy, which revealed a small duodenal ulcer and numerous 5—to 10-mm shallow ulcers in the ascending colon and caecum. A colon biopsy showed focal areas of erosions associated with mild to moderate acute inflammatory cell infiltrate. PAS histochemical stain highlights organisms with features compatible with Entamoeba histolytica. He was treated with Metronidazole for ten days.
Discussion: As amebiasis comes with non-specific GI symptoms like diarrhoea and abdominal pain, there can be a delay in getting a diagnosis. Chronic intestinal amebiasis can lead to iron deficiency anaemia, intestinal perforation, or liver abscess. Therefore, it is essential to consider amoebic colitis as the cause of chronic iron-deficiency anaemia.
PP-03-041
Cured intermediate stage hepatocellular carcinoma (HCC) after single session of transarterial chemoembolization
Boy Hutaperi1 and Putut Bayupurnama2
1Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia;2Division Of Gastroenterology and Hepatology, Gadjah Mada University School of Medicine, Sardjito Hospital, Yogyakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Intermediate stage HCC comprises wide variety of diease phenotype. TACE is the standard of care, instead of refinement in technique and patient selection, it remains non-curative.
Case report: A sixty-two years old female suffered dull pain around right abdomen for one month. She was controlled diabetic and negative for neither hepatitis B nor C. Physical examination found pale skin, tender hepatomegaly. Icteric, ascites and other sign of chronic liver were negative. Complete blood count showed hemoglobin 8.6 g/dL, negative finding of HBsAg, Anti-HBc and Anti-HCV, normal transaminase while liver and renal function preserved. AFP level was 1.79 ng/mL. MSCT showed right lobe liver mass, involving segmen 5,6,7 aproximately 9,0 x 8.0 x 10.0 cm fulfilled LIRADS 5 criteria, without portal vein thrombus or metastasis. Liver biopsy revealed hepatocellular carcinoma.
Selective TACE was opted as treatment modality using doxorubicin and cisplatin. Two month after TACE, MSCT showed necrotic area developed at mass location in segmen 6, next TACE was holded. Subsequent four month MSCT revealed the mass was disappeared. Patient stated as cancer free.
PP-03-042
Ruptured Abdominal Aortic Aneurysm Masquerading As Upper Gastrointestinal Bleeding: A Case Report
Edward Louie Ignacio, Tristan Colasito and Herlyn Abasolo-Dumaguin
Cebu Velez General Hospital, Cebu City (Capital), Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Abdominal aortic aneurysm (AAA) is the weakening and dilatation of the abdominal aorta and is usually asymptomatic. When ruptured, it usually presents with severe abdominal pain and hypotension requiring emergency repair. However, gastrointestinal bleeding is an uncommon presentation for ruptured AAA unless with confounding complication of a rare aortoenteric fistula.
Case Description: Presenting a case of 71-year-old female who came in with multiple episodes of hematemesis associated with epigastric abdominal pain, managed initially as upper gastrointestinal bleeding wherein esophagogastroduodenoscopy was done revealing a large submucosal extrinsic mass compressing the proximal gastric wall. CT scan of whole abdomen was done revealing a ruptured AAA with large paraaortic hematoma closely adjacent to the stomach. Immediate surgical referral for repair of ruptured aneurysm was done, however due to financial constraints and poor prognosis, opted to sign advanced directives. Patient eventually expired.
PP-03-043
Recurrent GI Bleeding in a Probable Hereditary Hemorrhagic Telangiectasia: A Rare Case
Fauzan Illavi and Muhammad Firhat Idrus
Departement of Internal Medicine, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia;Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Hereditary Hemorrhagic Telangiectasia (HHT) is a rare genetic disorder with myriad bleeding manifestations. The manifestations encompass epistaxis to GI or even intracranial bleeding.
Case Illustration: A-33-year-old male came with recurrent melena and severe microcytic anemia. The melena was intermittent without other bleeding manifestations. He had microcephaly without arterio-venous malformation (AVM) in his brain. His sister also had multiple melena episodes and microcephaly. Laboratory panel showed a very low Hb (Hb 5 g/dL) and mild azotemia. Upper and lower GI endoscopy showed no source of bleeding. Enteroscopy showed enteritis and multiple telangiectasias throughout jejunum and proximal ileum without active bleeding. Abdominal CT angiography did not show any abnormality. No genetic examination was performed. Intravenous PPI was administered until the bleeding subsided and the patient was discharged.
Discussion: Small bowel bleeding is a rarer GI bleeding and should be considered as one of the HHT manifestations. The diagnosis of HHT can be made using Curacao criteria: recurrent epistaxis, mucocutaneous telangiectasia, GI telangiectasia or AVM in any organ (lung, liver, brain, spine), and a first degree relative of HHT. This patient met 2 criteria, thus classified as Probable HHT. The gene mutation in HHT includes Endoglin (ENG, type I HHT) and Activin receptor-like kinase-1 (ACVRL1, type 2 HHT). Unfortunately, genetic mutation assessment could not be assessed due to inavailability of these panels. HHT is a rare genetic disorder that might cause recurrent GI bleeding. The use of Curacao Criteria is vital to diagnose this syndrome, especially when genetic mutation is not available.
PP-03-044
Chronic Radiation Proctitis with Giant Ulcer of The Rectum: A Delayed and Fatal Radiotherapy Complication
Fauzan Illavi and Rabbinu Rangga Pribadi and Saskia Aziza Nursyirwan
Departement of Internal Medicine, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Bekasi, Indonesia;Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Chronic radiation proctitis (CRP) is a delayed radiotherapy complication in many gynecological cases. CRP may induce colon ulceration, GI bleeding, and perforation if left untreated.
Case Illustration: A-49-year-old female came to the ER with worsening bloody, abdominal pain and preshock condition. She had endometrial cancer with history of debulking total hysterectomy, chemotherapy, and external beam radiotherapy (EBRT). Intermittent diarrhea appeared six months after she completed EBRT and progressed to hematochezia. Laboratory panels showed severe microcytic anemia (Hb 5.6 g/dL), mild thrombocytopenia, and low eGFR (20.8). Colonoscopy revealed multiple telangiectasia from sigmoid to the rectum, multiple ulceration, and giant ulcer of the rectum. Histopathology displayed ulcerative tissue with accumulation of acute and chronic inflammatory cells which tested negative for Tuberculosis. Sucralfate enema was administered and hematochezia resolved, however the patient’s condition worsened because of sepsis due to urinary tract infection.
Discussion: Radiotherapy in gynecological cancer therapy can lead to acute or chronic proctitis. The diagnosis might be delayed as in CRP the symptoms usually occur three months after the first radiotherapy. The patient presented with late onset of proctitis radiation, manifesting as CRP with severe anemia requiring blood transfusion. The grading of CRP in this patient was considered severe because it had caused deep ulceration, bleeding requiring transfusion, and abdominal pain. Argon plasma coagulation can be performed if active bleeding is found during colonoscopy. As no clinical colon perforation was present, surgery was not warranted and close monitoring was undertaken to monitor this complication.
Keywords: radiation, proctitis, hematochezia, endometrial cancer
PP-03-045
The efficacy of alternative therapies for glucocorticoid-resistant cases of IgG4-related disease: Two case studies
Ken Ito1, Takehisa Ogura2, Michihiro Saito1, Yuto Yamada1, Shinya Tajima1, Masataka Kurihara1, Yousuke Okamoto1 and Manabu Watanabe1
1Division of Gastroenterology and Hepatology, Toho University Ohashi Medical Center, Tokyo, Japan;2Division of Rheumatology, Toho University Ohashi Medical Center, Tokyo, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: IgG4-related disease (IgG4-RD) typically responds well to glucocorticoid therapy, but retroperitoneal fibrosis (RPF) may develop during the course. Here, we report two cases where alternative therapies effectively managed for IgG4-RD.
Case Description
Case One: A 62-year-old male presented with a 67mm pancreatic head mass and enlarged lymph node. Diffusion-weighted imaging MRI suggested pancreatic cancer, but EUS-FNA revealed no malignancy. IgG4 level was 493mg/dl, diagnosing Type 1 autoimmune pancreatitis and treated with glucocorticoids. Prednisolone (PSL) initially initially improved symptoms. However, tapering to 5mg caused retroperitoneal fibrosis (RPF) and left hydronephrosis. The PSL dose was increased again, but azathioprine was added since there was no change in RPF and hydronephrosis. Currently, RPF has decreased, with stable hydronephrosis and renal atrophy.
Case 2: An 87-year-old male with post-gastric cancer surgery had CT findings of pancreatic lesions. EUS-FNA found no malignancy, but imaging confirmed locally advanced pancreatic ductal cancer. Treatment with gemcitabine and nab-paclitaxel showed no tumor progression. During treatment, transient salivary gland swelling, RPF, and IgG4 elevation (394 mg/dl) were observed, leading to a diagnosis of IgG4-RD. We selected intravenous cyclophosphamide treatment due to diabetes mellitus and glaucoma but discontinued it because of the development of peripheral neuropathy. Treatment was then switched to a combination therapy of PSL and mycophenolate mofetil (MMF). Currently, RPF has significantly improved, and observation continues with MMF monotherapy.
Discussion: When RPF appears during glucocorticoid tapering or observation in IgG4-RD, it is important to consider other options, such as azathioprine or MMF.
PP-03-046
Duodenal Ulcer Developed as a Complication of Tsutsugamushi disease, A Korean Case
Jin Ook Jang
Pusan Nation University Yangsan Hospital, Yangsan, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Scrub typhus, or Tsutsugamushi disease, is caused by the transmission of Orientia tsutsugamushi usually through chigger mite bites. Most cases present with fever, chills, and a skin rash, and respond well to antibiotics. In cases of severe tsutsugamushi infection, various organs may be affected. We report a case of duodenal bleeding following a tsutsugamushi infection.
Case Description: In November 2023, a patient presented to the emergency department with recurrent abdominal pain and diarrhea, which had persisted for seven days. Computed tomography imaging demonstrated wall thickening of the colon, admitted to the gastroenterology department for suspected infectious diarrhea.
Following hospitalization, a skin rash and eschar were observed, leading to the suspicion of O. tsutsugamushi. This was confirmed by the detection of O. tsutsugamushi Ab (IFA) Positive (1:5120), and doxycycline was promptly initiated. The diarrhea improved as the fever disappeared.
On the 4th hospital day, he complained of heartburn and melena. A gastroscopy revealed the presence of a semicircular ulcer in the duodenal bulb. Despite the administration of a high-dose proton pump inhibitor (PPI), the melena persisted. On the following day, exposed blood vessels were identified, and hemostasis was performed. A follow-up endoscopy conducted one week later demonstrated the absence of further bleeding, and the patient was subsequently discharged.
Discussion: It is possible for patients to develop duodenal ulcers as a result of tsutsugamushi infections. Even after antibiotic treatment has been initiated and the fever has subsided, they may continue to experience heartburn and duodenal ulcer bleeding.
PP-03-047
Diagnostic and management dilemmas in stump appendicitis; A retrospective series of cases
Shehan Koshila Jayaweera, A Jayathilake, S Shrishankar and S Thalgaspitiya and K Senanayake
University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Stump appendicitis is a rare complication in post-appendectomy patients. We report four cases presented with stump appendicitis in a review of diagnosis and management in a resource-scarce setting.
Cases: A 48-year-old male patient with a history of open appendectomy 8 years ago was admitted with classic clinical symptoms and signs of acute appendicitis with elevated inflammatory markers. An ultrasound scan of the abdomen revealed gross inflammatory changes with fluid collection in the right inguinal fossa region. He underwent completion open appendectomy and uneventful post-operative recovery.
A 70-year-old female with multiple co-morbidities, who underwent open appendectomy 10 years back admitted with similar symptoms. She had elevated inflammatory markers and the ultrasound was unremarkable. Since she had persistent symptoms despite supportive care a CT scan was done and revealed stump appendicitis with surrounding inflammation. She was treated with 5 days of IV antibiotics and complete recovery achived.
Two male patients aged 18 and 25 respectively who underwent laparoscopic and open appendectomy 8 months and 3 ½ years back, presented with the same clinical presentation had elevated inflammatory markers and ultrasonographic inflammatory changes in RIF suggestive of stump appendicitis. Both were treated with intravenous antibiotics and completely recovered.
Discussion: The exact clinical diagnosis of stump appendicitis is supported by sonographic evidence which is even imperative due to difficulty in adequate visualization since most appendicular stumps <5mm in size. Even though Open/laparoscopic completion appendectomy is considered the treatment of choice, depending on patient-related risk-benefit factors conservative management with antibiotics can also be considered.
PP-03-048
Importance of multi-disciplinary approach in management of patients with Familial Adenomatous Polyposis (FAP) syndrome
Shehan Koshila Jayaweera, K Senanayake, SPB Thalgaspitiya, S Shrishankar and AB Jayatillake
University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Familial Adenomatous polyposis (FAP) syndrome is a rare genetic disease of autosomal dominant inheritance characterized by >100 polyps in the colon with a high risk of developing colorectal carcinoma. Diagnosed patients with classic FAP syndrome need to be evaluated for extra-colonic and extra-intestinal manifestations. Multi-disciplinary team (MDT) involvement in deciding non-surgical and surgical management, arranging family screening, and close follow-up is beneficial.
Case report: A 33-year-old previously healthy male patient presented with frequent fresh per rectal bleeding and passage of fleshy parts during defecation. He also had symptomatic moderate anemia with recent onset weight loss of >10 kilograms and a paternal side family history of deaths due to unevaluated abdominal malignancies. Numerous size polyps >100 involving the whole length of the colon were noted in colonoscopy and diagnosis of FAP was established. Multiple small benign gastric polyps during Oesophagoduodenoscopy, benign-looking pancreatic cysts in MRCP, and congenital retinal pigment hypertrophy on eye screening were detected as other manifestations. He underwent laparoscopic-assisted total proctocolectomy with ileoanal pouch anastomoses and currently has a satisfactory quality of life. MDT involvement and patient and family concerns are considered in all steps of management, screening, and follow-up plans. His sister was diagnosed having the same syndrome and referred to the oncology team for novel non-operative management options to minimize disease progression since she didn’t consent to surgery.
Discussion: Since patients with FAP syndrome carries a high risk of numerous malignancies, multi-disciplinary involvement in decision-making is always beneficial in formulating overall management, screening, and follow-up plans.
PP-03-049
Adult Ileocolic intussusception: A rare presentation of a common malignancy
Shehan Koshila Jayaweera, K Senanayake, AB Jayatillake, SPB Thalgaspitiya and S Shrishankar
University Surgical Unit, Teaching Hospital Anuradhapura, Anuradhapura, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Ileo-colic intussusception is a rare cause of intestinal obstruction in adults. In contrast to children which is mostly idiopathic, in adults, this condition is always associated with a definitive etiology. Clinical presentation can vary depending on the cause and individual symptoms.
Case report: A 44-year-old female patient with a background history of hypothyroidism presented with recent onset blood and mucous diarrhea, vomiting with moderate abdominal distension, and fever for 3 days duration admitted to medical casualty for further management of clinically suggestive dysentery. She had moderate anemia and slightly elevated inflammatory markers. Since she was poorly improved with antibiotics and supportive therapy, an abdominal ultrasound scan with plain X-rays was done and features of small bowel obstruction were noted. Urgent contrast-enhanced CT scan abdomen revealed evidence of ileocolic intussusception and she underwent emergency exploratory laparotomy and right hemicolectomy. Histological assessment of the resected part of the bowel showed 2cm size moderately differentiated invasive mucinous carcinoma with clear resection margins and 1 out of 14 lymph nodes containing tumor deposits in the specimen. She was referred for oncological management following uneventful post-surgical recovery.
Discussion: Clinical diagnosis of adult intussusception is relatively difficult due to non-specific symptoms upon initial presentation. Radiological diagnosis with contrast CT scans of the abdomen is helpful in definitive diagnosis. Timely surgical interventions are essential.
PP-03-050
Epidermoid cyst in the Ileocolic junction presented as a large abdominal mass during pregnancy
Shehan Koshila Jayaweera, K Senanayake, SPB Thalgaspitiya, AB Jayatillake and S Shrishankar
University Surgical Unit, Teaching Hospital Anuradhapura, Colombo, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Epidermoid cysts are benign encapsulated lumps filled with keratin materials that are usually found in skin. This is a rare case of an epidermoid cyst found in ileocolic area adjacent to appendix presented during pregnancy.
Case report: A 36 year old multiparous female in her first trimester of third pregnancy presented with gradually enlarging mobile right sided abdominal lump with on and off pain for 3 weeks duration. On examination there was a mobile, firm palpable lump measuring 8cm×6cm in the right hypochondriac and lumbar regions. It was neither attached to skin nor solid organs clinically. She underwent ultrasound assessment and suggested Magnetic Resonant (MRI) scan for further evaluation to rule out Gastro-intestinal stromal tumor (GIST). MRI scan was done and diagnosis of duodenal duplication cyst was given. Patient underwent diagnostic laparoscopy in her second trimester due to rapid enlargement of the concerned lump.
Intraoperatively, large well circumscribed mass measuring 13cm×12cm attached to ileo-colic and appendicular wall was noted and converted to open mini laparotomy. Lesion was carefully dissected from the serosa of the concerned bowel and appendix, and sent for histology. Histology revealed a benign epidermoid cyst arising from Ileocaecal wall filled with keratin material.
Discussion: Epidermoid cyst related to ileocaecal wall and appendix is unique with only few related cases in published literature. It is important as a rare benign differential diagnosis in mobile abdominal lumps with rapid enlargement.
PP-03-051
Treatment of Pyoderma Gangrenosum as an Extraintestinal Manifestation of Ulcerative Colitis
Alvin Johan and Saskia Aziza Nursyirwan
Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Pyoderma gangrenosum (PG) is an uncommon neutrophilic inflammatory skin condition. Up to 70% of reported PG cases are associated with autoinflammatory conditions such as inflammatory bowel disease (IBD) or inflammatory arthritis.1 The treatment of PG remains challenging due to the lack of generally accepted therapeutic guidelines.2,3
Case Description: A 19-year-old woman came to the emergency department with complaints of bloody stool and diarrhea that started one week before admission. She also experienced abdominal pain, polyarthralgia, and fever. There were painful skin ulcers in these last two weeks. On physical examination, we found tachycardia and a fever of 39,2 °C. There were well-defined ulcerations with a surrounding zone of erythema on the left chest and left groin. Laboratory examination showed anemia with hemoglobin of 7.9 g/dL and leukocytosis of 13,910 cells/μL. Stool examination showed stool with mucous and blood, with high leukocytes and erythrocytes. Colonoscopy revealed proctocolitis with continuous lesion extending from rectosigmoid to transverse colon with polypoid appearance. Tests for possible bacteria or parasite infections were negative. Histopathology examination found ulcers with distorted crypts. The swab on the ulcer bed found no bacterial growth. Diagnosis of ulcerative colitis with pyoderma gangrenosum was made. Hence, she was started on a course of oral steroids.
PP-03-053
Clinical case of radiofrequency ablation of recurrent ampullary adenoma
Valeriia Kamalova, Evgeniy Solonitsyn, Sabina Seyfedinova and Dmitriy Baranov
Almazov Nmrc, Saint Petersburg, Russian Federation
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Ampullary adenomas is extremelly rare but all of them should be removed if detected. The range of surgical interventions is extremely wide from pancreaticoduodenal resection to ablation techniques depending on the clinical situation.The presented case describes a long-term recurrence of an ampullary adenoma with successful resolution after RFA. 83 years old patient has a complaint of recurrent epigastric pain. He was diagnosed with ampullary adenoma in 2009 and underwent duodenotomy with excision of adenoma.After 8 years recurrence was noted and endoscopic ampulectomy was performed. In 2024 he underwent gastroscopy on which resected area was seen with scarring changes and enlarged villi. Biopsy was taken and tissue was suspected on adenoma with high grade dysplasia. EUS was performed to assess intraductal spread where dilated common bile was seen with hypoechogenic tissues in the terminal part.A decision was made to perform intraductal radiofrequency ablation. ERCP with intraductal ablation was performed and a plastic biliary stent was installed. In the postoperative period transient hyperamylasemia without clinical manifestations was noted. At the repeated ERCP cholangiocopy was performed on which scar tissues with enhanced vascular pattern was seen. A forceps biopsy was performed and a short plastic stent was placed. At pathological examination the samples consist of focal fibrosis so there were no data for adenoma recurrence.The patient is under outpatient observation now, he has no complaints. The described clinical case demonstrates the use of the latest endoscopic techniques for preoperative diagnosis and minimally invasive intervention for successful treatment of recurrent course of ampullary adenoma.
PP-03-054
Chronic Dysphagia due to an Esophageal Stricture: A Case Report on Lymphocytic Esophagitis
Xuan Yu Tan1, Sagar Sharma1, Heather Heng2 and Garrett Kang3
1Yong Loo Lin School of Medicine, National University of Singapore, Singapore;2Department of Pathology, Sengkang General Hospital, Singapore;3Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Lymphocytic Esophagitis (LE) is a rare form of chronic esophagitis, commonly presenting with dysphagia. Features of LE endoscopically include esophageal rings, linear furrows, whitish exudates, and esophageal strictures. It is characterised histologically by increased peripapillary/intraepithelial lymphocytes. The pathophysiology and aetiology of the disease are poorly understood, but has been associated with inflammatory bowel disease.
Case Description: We present a case of a 67-year-old female who previously presented to another hospital in 2018 with chronic dysphagia. Her upper endoscopy and barium meal were normal. High-resolution manometry indicated non-specific esophagogastric junction outflow obstruction, and endoscopic ultrasound showed no abnormalities at the gastro-esophageal junction.
The patient now presented again to our hospital in 2023 with worsening daily esophageal dysphagia. She underwent an esophagogastroduodenoscopy (Figure 1) which revealed a stricture in the upper oesophagus, and patchy esophagitis in the mid and lower esophagus. Biopsy was negative for malignancy, and showed increased intraepithelial lymphocytes without significant eosinophils or neutrophils. There was minimal spongiosis. GMS stain was negative for fungal organisms.
Taking into account the overall findings, our patient was treated for LE with a course of omeprazole and offered endoscopic dilatation.
PP-03-055
Dysphagia from Esophagitis Dissecans Superficialis in a patient with strong smoking history, improved with omeprazole
Rui Kyi Ng1, Ethel Ng1, Issam Jajeh2 and Garrett Kang3
1Yong Loo Lin School of Medicine, National University of Singapore, Singapore;2Department of Pathology and Laboratory Medicine, Sengkang General Hospital, Singapore;3Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Esophagitis dissecans superficialis (EDS) is a rare and benign disease, usually presenting with dysphagia. It is characterized by sloughing of the esophageal mucosa. There are associations of EDS with various medications, chemical irritants, dermatological and autoimmune diseases, but it can also be idiopathic.
Case Description: Our patient is a 53-year-old female presenting with daily esophageal dysphagia over the past 2-3 years without heartburn or regurgitation. She is a chronic smoker of at least 15 pack years and consumes a bottle of wine daily. Esophagogastroduodenoscopy (Figure 1) revealed sloughy mucosa and whitish desquamation seen in the lower & mid esophagus. Biopsy revealed basal hyperplasia, severe hyper parakeratosis and splitting. There was no dysplasia, fungal stain was negative. She was started on a course of omeprazole 20mg twice daily and had significant symptomatic improvement, now with symptoms occurring only 2-3 times a month.
PP-03-056
Phlegmonous gastritis in an immunosuppressed cirrhotic patient treated with antibiotics
Zihan Song1, Shu Ting Chong1 and Garrett Kang2
1Yong Loo Lin School of Medicine, National University of Singapore, Singapore;2Department of Gastroenterology & Hepatology, Sengkang General Hospital, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Phlegmonous gastritis (PG) is a rare and potentially fatal infection of the stomach wall, with a 10-54% mortality rate. Patients often present with epigastric pain, fever, vomiting, or bleeding. Although the pathophysiology is unknown, associated risk factors with PG include alcohol and immunodeficiency. Antibiotic treatment is often successful, although surgery may be required.
Case Description: Our patient is a 52-year-old male, with a background of recently diagnosed ANCA & PR3 positive vasculitis with IgA nephropathy, on treatment with prednisolone & cyclophosphamide. He also had a recent diagnosis of Child B NASH cirrhosis. Esophagogastroduodenoscopy (Figure 1A) was performed for variceal screening and bloating, which revealed small varices and pangastritis. On physical examination, the patient’s abdomen was distended but non-tender. Biopsies of the stomach showed subepithelial congestion with mixed acute & chronic inflammatory cell infiltrate with neutrophils seen, and destruction of gastric glands. This was suggestive of active gastritis suspicious for PG. Non-contrast Computed Tomography of the abdomen showed diffuse thickening of the mid to distal stomach wall (Figure 1B).
PG was diagnosed based on a combination of imaging, endoscopic and histological findings. In view of the patient’s immunosuppressed status, he was treated with a course of antibiotics with ciprofloxacin and metronidazole for 2 weeks. Our patient remained well clinically on follow up.
Discussion: This case demonstrates PG in a well but immunosuppressed patient with cirrhosis and vasculitis, that resolved with antibiotics therapy. As PG is potentially fatal, early diagnosis and treatment is crucial, even in an asymptomatic and well-appearing patient.
PP-03-057
Nintedanib-related colitis mimicking inflammatory bowel disease improved with Beclomethasone dipropionate
Jong Kyu Kim, Yong Sung Choi, Wan Jung Kim, Hyung-Joong Jung and Eun Joo Kim
Daehang Hospital, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Nintedanib is an intracellular inhibitor that targets multiple tyrosine kinases and has been shown to reduce the progression of idiopathic pulmonary fibrosis. However diarrhea was reported the most common adverse effect and led to discontinuation and reduction of nintedanib. Some case reports suggest that steroid may be a treatment for nintedanib-related colitis.
Case Description: A 35-year-old male was diagnosed with idiopathic pulmonary fibrosis 4 years ago. He had been taking nintedanib (150mg twice daily) after acute exacerbation 10 months ago. 3 months later, diarrhea and abdominal pain started. His symptoms had persisted despite taking anti-diarrheal medications. Infectious diseases were excluded from blood test and stool test. Colonoscopy revealed longitudinal linear ulcerations with friability on the transverse colon mimicking Crohn’s disease. Histopathology showed hyperplastic mucosa with chronic colitis and erosion. Nintedanib continued for idiopathic pulmonary fibrosis. He was prescribed beclomethasone dipropionate (5mg once daily). After 1 month, diarrhea improved without anti-diarrheal medications. Follow up sigmoidoscopy showed mild diffuse edematous mucosal change from transverse colon to sigmoid colon.
Discussion: Nintedanib was effective for idiopathic pulmonary fibrosis. However the adverse effect of nintedanib was mainly gastrointestinal problems, particularly diarrhea. Although the mechanism of diarrhea was unclear, metabolites of nintedanib could be directly caused damage to the intestinal epithelium. In some reports, systemic steroid was effective for nintedanib-related colitis. Beclometasone dipropionate is synthetic glucocorticosteroid which has a powerful local anti-inflammatory effect but little systemic effect. Beclometasone dipropionate may be an option for treatment of nintedanib-related colitis ineffective to anti-diarrheal medications.
PP-03-058
Complete CLOSURE USING MANTIS CLIPS After Endoscopic Submucosal Dissection (ESD) for duodenal adenoma
Kitakyushu Municipal Medical Center, Kitakyusyu-shi, Fukuoka, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Endoscopic submucosal dissection (ESD) can lead to bleeding and perforation, especially in the thin-walled duodenum. Secure closure of mucosal defects is crucial. We report a case of successful complete closure using Mantis clips after duodenal ESD.
Case Description: A 62-year-old male underwent upper gastrointestinal endoscopy, revealing a semi-circumferential adenoma in the descending duodenum, adjacent to the papilla. ESD was deemed feasible despite the challenging location.
Procedure: A pancreatic stent was placed to prevent pancreatitis. ESD was performed under general anesthesia, including circumferential dissection. The dissected area was closed using 11 Mantis clips and 6 Sure Clips, avoiding the papilla.
Postoperative Course: The patient experienced pain but no serious complications. Oral intake began on day 10, and discharge occurred on day 17. Follow-up endoscopies at 2 weeks and 2 months showed mild narrowing, but a standard endoscope passed without difficulty.
Discussion: Clipping is crucial for preventing complications after duodenal ESD. Mantis clips, designed for sealing mucosal defects, offer easier and quicker application compared to conventional clips. They can approximate larger wound areas, enabling treatment of even large, near-circumferential duodenal tumors. This case demonstrates the potential of Mantis clips in achieving complete closure after duodenal ESD, preventing complications in challenging cases.
PP-03-059
EUS-Choledochoduodenostomy as Salvage Therapy for Failed ERCP in Malignant Biliary Obstruction
Hendra Koncoro
Tzu Chi Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Endoscopic retrograde cholangiopancreatography (ERCP) is the treatment of choice for biliary obstruction. However, endoscopic biliary drainage may be unsuccessful in some patients. EUS- guided biliary drainage (EUS-BD) has emerged as an alternative to percutaneous transhepatic biliary drainage (PTBD) with a high technical and clinical success rate, low risk of complications and a better quality of life.
Case description: We described a case of endoscopic ultrasound-guided choledochoduodenostomy (EUS-CDS) from the first portion of the duodenum using self-expandable metal stent (SEMS) in a patient with distal malignant biliary obstruction in who ERCP was failed due to neoplastic invasion of the distal bile duct. A 75-year-old woman with a complaint of abdominal pain and jaundice was referred to our polyclinic from oncologist. The patient was diagnosed with stage IV urinary bladder cancer metastasis to vertebra and distal bile duct. The patient was suffering from obstructive jaundice and underwent endoscopic biliary drainage. However cannulation was not achieved on this setting and EUS- BD from the first portion of the duodenum was performed (Figs 1, 2).
Discussion: Transhepatic biliary drainage has been the most common procedure for the treatment of malignant biliary obstruction in cases which ERCP fails due to tumor infiltration of the distal CBD. During the last decade the development of EUS has implied an alternative for biliary drainage in cases of failed ERCP, demostrating advantages over trans hepatic biliary drainage.
PP-03-060
Hidden danger: Aortoenteric fistula as the cause of gastrointestinal bleeding
Hendra Koncoro, Andrew Jackson and Sugianto Santoso
St Carolus Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Aortoenteric fistula (AEF) is a rare and potentially fatal condition where the aorta forms an abnormal connection with the gastrointestinal (GI) tract, often leading to severe GI bleeding. Prompt identification and treatment are crucial for improving patient outcomes.
Case Report: A 46-year-old man presented at St. Carolus Hospital’s Emergency Unit with nausea, upper abdominal pain, and dizziness persisting for 9 hours. He reported occasional black, blood-mixed stools over the past month. On examination, he was conscious and hemodynamically stable with anemic conjunctiva and epigastric tenderness. Lab results showed anemia (Hb 8 g/dL) leukocytosis (14,970/uL) and hypokalemia (K 3.3 mmol/L). Gastroscopy and colonoscopy ruling out active bleeding. CT abdomen with contrast detected an abdominal aortic dissection extending subdiaphragmatically to the bifurcation, alongside a left abdominal aortic aneurysm with suspected thrombosis. CT angiography confirmed the dissection from the left subclavian artery to above the iliac bifurcation (DeBakey III, Stanford B). Collaboration with vascular thoracic surgeons for endovascular aortic repair (EVAR) improved his condition, leading to discharge.
Discussion: AEF diagnosis requires a high index of suspicion, as the classic clinical triad of bleeding, abdominal pain, and palpable abdominal mass may not be present. AEF should be considered in AAA patients presenting with GI bleeding. Timely diagnosis is challenging without prior AAA identification. EVAR is recommended for patients unfit for complex open surgery. Untreated AEF is almost always fatal, highlighting the need for early diagnosis and intervention.
PP-03-061
Autoimmune Pancreatitis with Pancreatic Pleural Effusion Successfully Treated with Steroid Therapy: Two Case Reports.
Hoshu Kurebayashi1, Hiroaki Kitae1, Kyoka Takatsuka1, Koki Yamamoto1, Yusaku Yokotani1, Mika Mazaki1, Naoto Watanabe1, Takaharu Yo1, Naoaki Akamatsu1, Tsugitaka Ishida2, Nobuhiko Yonekura2, Masanobu Katayama2 and Hiroaki Yasuda2
1Omihachiman Community Medical Center, Omihachiman, Japan;2Saiseikai Shiga Hospital, Ritto, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: There are few reports of autoimmune pancreatitis (AIP) with pancreatic pleural effusion. This report presents two cases of AIP with pancreatic pleural effusion successfully treated with steroid therapy.
Case Description:
Case 1: A male in his fifties with a history of alcoholic acute pancreatitis presented to the ER. A CT scan showed recurrent acute pancreatitis with massive pleural effusion in the left thoracic cavity. A chest drainage tube was inserted, but its efficacy was limited. Elevated amylase levels were confirmed by thoracentesis. MRCP and EUS revealed diffuse pancreatic swelling and a high serum IgG4 level, leading to the diagnosis based on the Japanese Clinical Diagnostic Criteria for AIP 2018. After prescribing prednisolone (50mg/day, initial dose), pleural effusion was rapidly resolved.
Case 2: A male in his seventies was referred to the outpatient with an elevated serum amylase level. A CT scan revealed pancreatic swelling and right pleural effusion. Elevated serum IgG4 and antinuclear antibody levels were also observed. ERCP and MRCP findings were consistent with AIP. Thoracentesis in right thoracic cavity proved elevated amylase level. Prednisolone (30mg/day, initial dose) was prescribed as a steroid trial. Two weeks later, a CT scan demonstrated the resolution of the right pleural effusion.
Discussion: Management of pancreatic pleural effusion may require various strategies, such as thoracentesis, endoscopic pancreatic duct drainage, administration of octreotide, and pleural surgery in challenging cases. This report suggests that diagnosis by thoracentesis to confirm elevated amylase levels and early steroid therapy can be beneficial in avoiding unnecessary interventions.
PP-03-062
Effective Endoscopic Submucosal Dissection of a Huge Esophageal Liposarcoma: A Case Report
Moonwon Lee1, Hyun Ji Lee2, Gwang Ha Kim1, Bong Eun Lee1, Sunghoon Kim1, Dong Chan Joo1, Deajin Jung1 and Cheol Woong Kim1
1Pusan National University Hospital, Busan, South Korea;2Pusan National University Yangsan Hospital, Yangsan, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
This case report presents the successful endoscopic submucosal dissection (ESD) of a well-differentiated esophageal liposarcoma in a 51-year-old male with persistent dysphagia. The cause was initially diagnosed as a 10 cm pedunculated lesion extending from the upper esophageal sphincter to the mid-esophagus. An ESD was chosen over traditional surgery because it is less invasive. The procedure involved a precise submucosal injection and excision with special techniques to manage bleeding from a central vessel. Despite the extraction challenges owing to the size of the lesion, it was successfully removed orally. A histopathological examination of the 8.3×4.2×2.3 cm specimen revealed the characteristic features of a well-differentiated liposarcoma, including MDM2 and CDK4 positivity. The follow-up revealed no recurrence, and active surveillance has been performed since. This report highlights the versatility of ESD in treating significant esophageal tumors and provides evidence for its efficacy as a minimally invasive alternative.
PP-03-063
A Gastric Magnetic Foreign Body Incidentally Detected Several Years after Ingestion
Moonwon Lee1, Hyun Ji Lee2, Gwang Ha Kim2, Bong Eun Lee1, Dong Chan Joo1, Deajin Jung1 and Sunghoon Kim1
1Pusan National University Hospital, Busan, South Korea;2Pusan National University Yangsan Hospital, Yangsan, South korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Foreign body ingestion is commonly seen in children. However, occasionally it may also be seen among adults and is often associated with intellectual disability, psychiatric disorders, and alcoholism. Ingestion of a magnetic foreign body may cause complications such as gastrointestinal tract perforation, wherein emergency endoscopic removal of the foreign body is generally required. Here, we report a rare case of a 59-year-old male with an intellectual disability and psychiatric disorder in whom metallic objects in the stomach cavity were accidentally discovered during abdominal CT. Esophagogastroduodenoscopy revealed several metallic objects attached to two magnets, which had been ingested several years before and had remained in the stomach cavity. The magnets and metallic objects were safely removed endoscopically using rat-tooth forceps without complications.
PP-03-064
Dissecting through the diagnostic complexities of solid-cystic pancreatic tumours
Wei-Qiang Leow and Samuel Jun Wei Tho
Singapore General Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: We dissect through the differential diagnoses for a solid-cystic pancreatic tumour, using a case of a pancreatic acinar cell carcinoma, to highlight morphological similarities and differences. We also discuss on use of immunohistochemistry, particularly BCL-10 and its pitfalls.
Case Report: We present a diagnostic dilemma of a forty-four year-old gentleman who presented with abdominal pain secondary to an 18cm solid-cystic pancreatic tumour. Histologically, the tumour was composed of epithelioid tumour cells with abundant granular cytoplasm and prominent nucleoli. Extensive necrosis and cystic degeneration was also noted. We discuss a list of differential diagnoses including neuroendocrine tumour, solid pseudopapillary neoplasm and acinar cell carcinoma. Initial immunohistochemistry revealed focal positivity for neuroendocrine markers and a moderately high cell proliferative index (Ki-67) of up to 35%. Although BCL-10 performed in our laboratory was negative, a second consultation at an overseas laboratory confirmed the diagnosis of pancreatic acinar cell carcinoma with a positive trypsin immunohistochemical stain.
Discussion: We discuss the morphological similarities and differences of solid-cystic pancreatic tumours, including the classical immunohistochemical profiles. We delve deeper into the reasons for the discordant BCL-10 immunohistochemistry findings.
Andhiky Raymonanda Madangsai and Muhammad Firhat Idrus
Pertamina Jaya Hospital, Jakarta, Indonesia;Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Familial adenomatous polyposis is an autosomal dominant polyposis syndrome characterized by mutations in the adenomatous polyposis coli gene1. If high-grade dysplasia is detected in any gastric polyp, endoscopic or surgical resection is recommended1. Endoscopic resection (ER) with bipolar snare, in which the electric current only passes through the tissue between the device’s two electrodes, is a prominent method used to prevent perforation due to electricity potentially. ER with bipolar snare is expected to have good treatment outcomes in 10–15 mm colorectal lesions, with high safety even without submucosal injection2.
Case Report: A 56-year-old female came with worsening epigastric pain around 3 years before hospital admission. The pain felt continuously, radiates from the back, chest, and lower abdomen. The patient complained about heartburn since a year ago, but it has gotten worse since 2 weeks. Nausea and vomiting occur when the pain is coming, food intake is around quarter portion meals per day. On March 2022, this patient underwent an EGD and the result was found polyp gaster. Patient referred to Cipto Mangunkusumo Hospital for esophagogastroduodenoscopy for polypectomi.
Polypectomy was performed with hot snare technique on the 10 largest polyps (size varies around 5-10mm). There was no active bleeding after the polypectomy.
Conclusion: In this case, the familial adenomatous polyposis being resected by hot snare (size approximately around 5-15 mm). By this case, hot snare resection techniques can be effectively used for polypectomy. However, more studies are necessary to evaluate any other resection methods.
PP-03-066
Surgery vs Non-Surgery Treatment for Diverticulosis in Elderly
Andhiky Raymonanda Madangsai and Haya Harareed
Pertamina Jaya Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Diverticulosis is a clinical condition in which multiple sac-like protrusions (diverticula) develop along the gastrointestinal tract. The majority of individuals with diverticulosis are asymptomatic but some other have symptom like diverticular bleeding1. Surgery is required for unwell patients or those with advanced disease2. The non-operative management begins with stabilization of the patient, obtaining pertinent history, and an appropriate work-up, which often includes technetium-tagged red blood cell scans, mesenteric angiography, and colonoscopy. For colonoscopy, these include cautery, epinephrine injection, and endoclips. These modalities may be utilized as a bridge to surgery, or in select instances as a definitive therapy obviating the need for surgery3.
Case Report: An 84-year-old female came with melena since 3 days before hospitalized. On 16th May 2024, this patient underwent an EGD and the result came with colon diverticulosis (suspected source of bleeding from diverticula, but currently no active bleeding) and third grade of internal haemorrhoids. The patient is planned to undergo a hemoclip or Argon plasma coagulafion (APC) if recurrent bleeding occurs. Laboratory result shows hemoglobin levels only 5,9 g/dL. 4 packs PRC transfusion was performed to this patient.
Conclusion: In this case, colonoscopy ligation performed for diverticulosis in elderly. Surgery is not the main option for this patient because the elderly are at high risk to do surgery. From this case, further research in medical management for diverticulosis in elderly still needs to be studied further.
PP-03-067
Twice tried, Twice failed… What next?
Rishikesh Malokar, Vishal Malokar, Sanjay Chandnani and Pravin Rathi
Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction and Aim: POEM usually has excellent clinical success.
In a study, the long-term clinical success of POEM in years 1, 2, 5, and 7 was 98%, 96%, 92%, and 91%, respectively. Two percent are non-responders at the primary level after the procedure. We describe a case of Achalasia Cardia Type II, who, in spite of undergoing POEM twice, did not have relief of symptoms and had clinical failure.
Case Description: 35 Y/ M Dysphagia x 1 yr UGIScopy (Outside) - Achalasia Cardia, Eckardt Score 8, HRM ( Outside), Basal LES pressure – High, IRP 43. Underwent Posterior POEM (Outside). Symptoms recurred after one month. In our hospital, ECKARDT- 6. UGI Scopy - Mild resistance at GE Junction Achalasia Cardia HRM- Raised IRP 17.5mmHg, ( Achalasia Cardia II) Underwent Anterior POEM. Symptoms recurred after one month. ECKARDT score 5 Current HRM Median IRP 14.36 mmHg, Ineffective oesophagal motility Planned for Pneumatic balloon dilatation.
If it fails, 3rd POEM…?
Discussion: Pathophysiology in recurrence could be due to the development of fibrosis and regeneration of fibres. Third POEM is technically challenging as there’s an issue with the unnatural scope orientation of submucosal fibrosis and an increased risk of bleeding.
Here, in this case we had exhausted our options and gave an option of PD.
In a multicentric study by Ichkhanian Y et al., clinical success of Repeat POEM> PD> LHM after failed POEM
Conclusion: Pathophysiology of recurrence and optimal management of such cases is still unclear. Data regarding3rd POEM is sparse
PP-03-068
Reversible cause of Gastroparesis: Disseminated Koch
Topiwala National medical college and BYL Nair Ch hospital, Mumbai, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Idiopathic gastroparesis includes broad category of aetiologies. Viral infections like EBV, CMV, Varicella, and HSV are known to cause gastroparesis. The complex mechanisms include neuroimmune response against the enteral nervous system and post-infectious dysautonomia. Tuberculosis causing gastroparesis is rare entity. We describe a case of Reversible Gastroparesis due to disseminated tuberculosis improved completely post anti-tubercular therapy
Case description: A 24-year-old female presented with abdominal pain and persistent vomiting for 1 year in 2019.
She also had intermittent fever, cough, and weight loss of 15kg in 1 year (2019)
HRCT chest and CT abdomen showed pulmonary Koch with periportal lymph nodes with urogenital Koch. Upper GIScopy (2019) showed food residue in stomoch.
Gastric emptying was markedly prolonged for both solid and liquid on scintigraphy.
Patient had repeated hospitalization for persistent vomiting. She received NJ feed initially then oral gastroparesis diet. No improvement. MTB with HR resistance detected in sputum.
The patient was started on a bedaquiline-based regimen with dietary modifications. The patient improved slowly over two years of therapy. Repeat gastroscopy, gastric emptying study (2024), and electrogastrography (EGG) (2024) were normal.
Discussion: In idiopathic gastroparesis, pathophysiology could be due to
Neuroimmune processes targeting ENS.
Systemic dysautonomia.
Condition is rarely reversible and requires lifelong dietary modifications and prokinetics.
Tuberculosis is a rare cause of gastroparesis. Gastroparesis needs to be considered a D/D for persistent vomiting. Antitubercular treatment can improve the neuroimmune process and motility.
Further microscopic, autoantibody and gastric motility studies required.
Conclusion: Disseminated Koch is reversible cause of gastroparesis
PP-03-069
CROHN’S DISEASE MIMICKING A PENETRATING SIGMOID TUMOR
Intan Aaroni Md Isa and Syuhada Dan Adnan
Gastroenterology and Hepatology Unit, Department of Internal Medicine, Hospital Sultanah Nur Zahirah, Kuala Terengganu, Malaysia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Crohn’s disease has been known to imitate other illnesses such as tuberculosis, diverticulitis and others including tumors. The usual site of colonic Crohn’s disease, as observed in the past, is at the ileocecal area and it is usually presented as a stricturing disease. Tumefactive Crohn’s disease is mostly infrequent, what more in the sigmoid area.
Case Description: We present a rare case of penetrating sigmoid pseudotumor of Crohn’s disease of a 59 years old gentleman who came with a 6 months history of diarrhoea and constitutional symptoms. Initial colonoscopy found mild colitis in the rectosigmoid area and biopsy revealed an active colitis with subtle chronicity where he was treated for infection. Subsequently, he developed clinical features of intestinal obstruction which emergency laparotomy done unveiled a tumor at sigmoid with infiltration to the bladder, and diversion transverse colostomy was decided intra-operatively. Colonoscopy post-operation found a rectosigmoid mass 20 cm from the anal verge and the biopsy showed an active colitis with mild chronicity. With the differential diagnosis of malignancy in mind, a repeated colonoscopy and biopsy were done. However, the verdict remained ambiguous. Consequently, the serial biopsy samples were sent for a second opinion from an IBD-Histopathologist which concluded the features of Crohn’s disease
Discussion: Dealing with tumefactive sigmoid lesion should warrant a higher clinicians’ suspicion of possibility of Crohn’s Disease, other than malignancy. Added to that, multiple biopsies reviewed by experienced pathologists would tremendously contribute for diagnosis.
PP-03-070
Endocytoscopy for optical biopsy of pediatric eosinophilic esophagitis
Anastasiya Merkulova
Clinical And Research Institute Of Emergency Pediatric Surgery And Trauma, Moscow, Russian Federation;Institute of continuing medical education and professional development, Pirogov Russian National Research Medical University, Moscow, Russian Federation;Morozov Children’s City Clinical Hospital, Moscow, Russian Federation
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Eosinophilic esophagitis (EoE) is one of the most common causes of dysphagia in adults and children. Pathomorphological diagnosis (at least 15 eosinophils) is essential and needs at least 6 biopsies from esophageal mucosa. The same - at follow-up endoscopies. Endocytoscopy claims to be the novel diagnostic technique for EoE verification in vivo.
Case report: A 13-year-old boy with complaints of abdominal pain and moderate swallowing difficulties was admitted to CRIEPST. Esophagogastroduodenoscopy was held twice: before the treatment and 1 month after. At initial endoscopy active EoE (E1R0E2F1S0), erosive HP-gastritis and acute ulcer of duodenal bulb were detected. After chromoscopy of the esophageal mucosa with 1% methylene blue endocytoscopy with optical magnification up to x520 was performed. Multiple cells with blue bilobed or bean-shaped nuclei and non-stained periphery (eosinophils) were identified among squamous epithelial cells (Fig.1). Biopsy was taken at the site of endocytoscopic observation for histological confirmation of EoE, which revealed eosinophilic infiltration (36-54 eosinophils) of the esophageal mucosa (Fig.2). After therapy with proton pomp inhibitors follow-up endoscopy with endocytoscopy detected isolated single eosinophils in esophageal mucosa (Fig.3). Histology confirmed the effective outcome: there were 0-4 eosinophils in biopsy samples from esophageal mucosa (Fig.4).
Discussion: This case demonstrates that esophageal eosinophilic infiltration in children can be revealed by endocytoscopy. It allows to decrease the number of biopsies, exclude false negative histological findings, estimate the effectiveness of treatment in children with EoE. This case is by far the first implementation of endocytoscopy in children in Russia. Further research is needed.
PP-03-071
An autopsy case of pancreatic cancer with bone marrow carcinomatosis : a case report
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Bone marrow carcinomatosis is very rare in pancreatic cancer, which can cause disseminated intravascular coagulation (DIC) and has poor prognosis. We report herein a case of pancreatic cancer with rapid clinical course in which bone marrow carcinomatosis was found at autopsy.
Case report: A 75-year-old man visited emergency department of our hospital with upper abdominal pain and fatigue lasting 10 days. Vital signs were normal. Laboratory test revealed abnormal liver function, thrombocytopenia, the presence of erythroblasts and postmyelocytes, and DIC. Computed tomography (CT) showed the mass of pancreatic body, multiple hepatic masses and only a few nodular shadows at lungs. Pancreatic cancer was suspected, but pathological examination could not be performed due to thrombocytopenia. Hypoxemia of unknown cause was gradually progress, and he died on the third day of admission. Autopsy showed a 35 mm mass in the body of the pancreas, and pathology showed poorly differentiated carcinoma. Similar tumor cells were found in the liver and lungs, and the bone marrow was replaced by tumor cells. In addition, there was tumor embolization in small intrapulmonary arteries, which could cause progressive hypoxemia. We diagnosed bone marrow carcinomatosis in pancreatic body cancer, which was considered to cause DIC and lead to hypoxemia and rapid death.
Discussion: This is the case that had a typical clinical course of bone marrow carcinomatosis, but we didn’t diagnose it before death. In case of pancreatic cancer with DIC, the possibility of bone marrow carcinomatosis should be should be considered in the differential diagnosis.
PP-03-072
Probiotics Effect in Liver Cirrhosis Patient : An Evidence Based Case Report
Dewi Mustikarani
Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Liver cirrhosis can progress to a neurological complication named hepatic encephalopathy. Cirrhosis kills 1.3 million individuals a year and affects over 160 million people. It is critical to discover efficient therapies to stop severe liver damage. Studies have shown that the progression of cirrhosis is associated with inflammatory changes and dysbiosis.
Case Description: A 22-year-old male patient with a previous history of liver cirrhosis was admitted to the emergency room with an altered mental status. The patient was previously given lactulose from his last admission two weeks ago, but he stopped taking lactulose for three days on his account and has not been able to defecate ever since. The patient’s has edema peritibial and massive ascites. The patient was then diagnosed with a third-degree hepatic encephalopathy. The patient was previously advised to take probiotics but has not yet taken them.
Discussion: The inclusion and exclusion criteria were met by two meta-analyses conducted by Yang et al. (2024) and Wibawa et al. (2023). Probiotics significantly reverse minimal hepatic encephalopathy (MHE), as demonstrated by Yang's research (RR 1.54, 95% CI: 1.03 to 2.32). Probiotics have been shown by Wibawa to considerably reduce serum ammonia as compared to a placebo (p = 0.04, 95% CI: –50.21 to –1.66).
Conclusion: The results showed that compared to a placebo or no treatment, probiotics significantly reverse MHE and reduce serum ammonia levels. Further research is encouraged to establish probiotics as one of the routine drug choices in liver cirrhosis.
Cytomegalovirus (CMV) Esophagitis Presenting as Upper Gastrointestinal Bleeding: A case report
Rangga Novandra, Rabbinu Rangga Pribadi and Robert Sinto
RSUPN Ciptomangunkusumo, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Cytomegalovirus disease of the gastrointestinal tract is a major cause of morbidity and mortality in immunocompromised patients. Esophagitis is the most common GI manifestation of CMV infection after colitis. Standard diagnosis requires biopsy and pathologic verification. Current medical treatment in this disease still consist of ganciclovir and valganciclovir.
Case Illustration: Male patients, 58 y.o, came to emergency department with complaints of hematemesis and melena. Patient denied any complaints of odynophagia and dyspaghia. He reported abdominal pain and jaundice since 5 months. The patient has a history of hematemesis, melena, and history of repeated hospitalization for transfusion since 10 years. He had no history of taking antiplatelet, oral anticoagulan or, using long-term NSAID.
In laboratory examination shows anemia, elevated serum transaminase and bilirubin. Result of HBs Ag, Anti-HCV, and Anti-HIV are nonreactive.
From endoscopic examination show solitary single transversal ulcer in the distal esophagus with firm demarcation, biopsy sample was taken with real time PCR quantitative CMV value 79.6 x 104. Patient received ganciclovir 500 mg iv/day, devide in 2 doses.
Discussion: Diagnosis of CMV esophagitis is based on clinical history, endoscopic features, and histopathologic features. Clinically, the most common symptoms of CMV esophagitis are odynophagia, dysphagia, and chest pain. Histopathology with specific immunohistochemical stains or deoxyribonucleic acid polymerase chain reaction using tissues are required for definitive diagnosis of CMV esophagitis.
Although histopathology with specific IHC stains is the gold standard for the diagnosis of CMV esophagitis, endoscopic features are important for empirical treatment prior to histopathologic diagnosis.
PP-03-074
Systemic Lupus Erythematosus in a Patient with Inflammatory Bowel disease: Co-existing or SLE in Evolution?
Bea Regine Panganiban, Janika Adrienne Balane and Ma. Lourdes Daez
Philippine General Hospital, Ibaan, Batangas, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE) are chronic immune disorders marked by relapses and remissions. The coexistence of these conditions is rare, with an estimated 0.4% prevalence of ulcerative colitis in SLE patients, and much less with Crohn’s disease. It represents a diagnostic challenge, as they have many similar gastrointestinal manifestations.
Case Description: A 20-year-old female was diagnosed with IBD in 2019 after presenting with persistent diarrhea, abdominal pain, and pallor. Initial workup revealed hemolytic anemia and thrombocytopenia, leading to a presumptive diagnosis of autoimmune hemolytic anemia. Despite negative autoantibody tests, high clinical suspicion of SLE prompted further evaluation. Radiologic and endoscopic findings were consistent with IBD, and biopsy showed chronic colitis without granulomas. Bacterial, parasitic, viral, tuberculous and malignant etiologies were also ruled out. Treatment with 5-ASA and steroids led to symptom resolution. However, in 2022, the patient was readmitted with edema and laboratory evidence of SLE, including positive ANA, elevated anti-dsDNA, decreased complement, and active urinary sediment. Although planned for pulse steroid therapy, the patient died from nosocomial pneumonia.
Discussion: This case highlights the uncommon association between IBD and SLE, prompting the possibility that our patient, despite initially negative autoantibodies, may have had a developing SLE that evolved into complete lupus over time. It underscores the importance of maintaining a high index of suspicion for SLE in IBD patients with complex or atypical presentations, as timely identification can significantly impact prognosis. Studies are needed to justify surveillance for autoimmune antibodies in patients with IBD.
PP-03-075
Navigating Risks: A Case of Esophageal Perforation During OTSC Application for ERCP-Related Duodenal Perforation
Bea Regine Panganiban, Carina Samone Gregorio and Eric Yasay
Philippine General Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Perforations during ERCP is a rare but dreadful complication. The advent of over-the-scope clips (OTSC) has enabled effective endoscopic management of iatrogenic perforations, achieving high success rates. However, complications can arise from its use.
Case Description: A 94-year-old female with hypertension and chronic kidney disease presented with abdominal pain and jaundice, and was diagnosed with choledocholithiasis on imaging. Initial attempts at ERCP failed, necessitating percutaneous transhepatic biliary drainage (PTBD). A year later, due to discomfort with PTBD, conversion to internal biliary stenting was desired. ERCP was performed, however intra-procedure, cholangiography demonstrated an abnormal perinephric gas shadowing prompting cessation of ERCP. Subsequent endoscopy confirmed a 2.0x1.5 cm perforation at the lateral duodenal wall. Attempts to repair the perforation with endoscopic hemoclips were unsuccessful, hence OVESCO was deployed, achieving good tissue apposition. Shortly after, the patient developed signs of pneumothorax, requiring chest tube insertion. On follow-up endoscopy, a 15mm esophageal mucosal tear was identified and closed with e hemoclips. The patient was conservatively managed and showed no signs of leakage on gastrointestinal series by day 6. She remained stable and was discharged on day 10, tolerating a full diet.
Discussion: With only two cases reported in literature to date, this case illustrates the rare occurrence of esophageal perforation due to OTSC deployment, emphasizing the risks associated with advanced endoscopic techniques particularly in elderly patients with comorbidities. While OVESCO clips are promising for closing gastrointestinal perforations, this case highlights the necessity for meticulous technique and recognition of potential complications.
PP-03-076
Rare Occurrence of Gastric and Gallbladder Metastases in Non-Small Cell Lung Carcinoma: A Case Report
Federico Iv Peralta, John Erwin Dumagpi, Raiza Rodriguez and Gerardo Cornelio
St. Luke's Medical Center - Global City, Taguig, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Gastrointestinal metastasis from lung cancer (GMLC), particularly non-small cell lung carcinoma (NSCLC), is a rare and complex condition. NSCLC primarily targets the lungs, but it can metastasize to the gastrointestinal tract, with gastric and gallbladder involvement being particularly uncommon. The rarity of gastrointestinal metastasis complicates diagnosis as it can mimic benign conditions, leading to potential delays in treatment. Symptoms such as gastrointestinal perforation, hemorrhage, and obstruction may occur, although many patients remain asymptomatic. Diagnostic tools including endoscopy, CT scans, and PET scans are essential for accurate identification. Despite advancements in diagnostics and treatment, the prognosis for NSCLC patients with gastrointestinal metastases remains poor.
Case Presentation: This report describes an 80-year-old female with Stage IVB NSCLC, who developed new abdominal symptoms. A routine surveillance PET scan revealed a new hypermetabolic lesion at the lesser curvature of the stomach and a distended gallbladder. Subsequent esophagogastroduodenoscopy and surgery identified a gastric ulcer and an inflamed gallbladder which were biopsied and removed. Histopathological examination revealed adenocarcinoma. Immunohistochemical staining (CK7, CK20, TTF1, and Napsin A) confirmed the diagnosis of gastric and gallbladder metastases from primary lung cancer. Following these findings, the patient’s treatment was adjusted to include Pemetrexed chemotherapy alongside continued Osimertinib.
Discussion: This case highlights diagnosing GMLC's clinical challenge due to its rarity and non-specific symptoms. Heightened clinical awareness and comprehensive diagnostics are crucial for NSCLC patients with new gastrointestinal symptoms. Understanding clinical presentations, diagnostic challenges, and management strategies for NSCLC gastrointestinal metastases are vital for improving outcomes and guiding future research.
PP-03-077
Herpes simplex oesophagitis in immunocompetent individuals
Zeeshan Pervaiz, Ahtsham Zafar, Asif Yasin and Ali Qamar
Walsall Healthcare Nhs Trust, Walsall, United Kingdom
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Herpes esophagitis commonly affects immunocompromised patients, especially those who have undergone organ transplants and are on antitumor drugs. Symptoms include chest pain, difficulty swallowing, and nausea or vomiting. Timely diagnosis is crucial for effective treatment, typically involving antiviral medications like acyclovir. Although rare in immunocompetent individuals, stress can reduce immunity and lead to herpes skin infections. Few cases of herpes esophagitis have been documented in immunocompetent patients.
Case Report: A 51-year-old man with a history of mild erosive gastritis and psychosis presented with a 5-day history of nausea and vomiting and was found unresponsive with a low GCS score. Initial assessment revealed electrolyte imbalance, acute kidney injury, elevated lactate levels, and raised inflammatory markers. A CT scan identified a cystic mass causing partial gastric obstruction. The patient required intensive care and was started on antifungal medication and acyclovir. An esophagogastroduodenoscopy revealed multiple superficial volcano-like ulcers, and biopsies confirmed herpes simplex virus (HSV) esophagitis. He was treated with intravenous acyclovir and fluconazole.
Discussion: HSV esophagitis typically affects patients with compromised immunity, but this patient had no clinical signs of immunosuppression. The severity of HSV infection is influenced by the site of involvement, immune status, and whether the infection is primary or recurrent. Endoscopy often reveals lesions in the distal or mid-esophagus. HSV-1 is primarily associated with herpes esophagitis, though rare cases of HSV-2 have been reported.
Conclusion: This case underscores the rare occurrence of herpes simplex esophagitis in immunocompetent individuals. Prompt antiviral therapy can effectively alleviate symptoms.
PP-03-078
A Unique Case of a Triple Primary Gastrointestinal Malignancy in a Filipino Male
Matthew Joseph Po and Jenny Limquiaco
Chong Hua Hospital, Cebu City (Capital), Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: The occurrence of multiple primary malignancy (MPM) is an uncommon scenario that is characterized by the presence of two or more malignancies that are histologically distinct. Although the frequency of MPM is increasing, cases of three primary gastrointestinal malignancies are exceedingly rare. This case report presents an unprecedented case involving hepatocellular carcinoma (HCC), gastric gastrointestinal stromal tumor (GIST), and pancreatic adenocarcinoma.
Case Presentation: A 68-year-old Filipino male was incidentally diagnosed with a hepatic mass during routine ultrasound. Contrast CT scan and percutaneous liver mass biopsy were both consistent with HCC. Surgical resection successfully removed the liver mass, but a gastric nodule was seen intraoperatively. The nodule was resected and sent for histopathology and immunohistochemical staining. Results were consistent with a gastric GIST, for which the patient received adjuvant treatment with Imatinib. Sixteen months later, a metachronous pancreatic adenocarcinoma was discovered. Radical surgery and adjuvant chemotherapy were administered, yielding a favorable postoperative course.
Discussion: Although there have been previous reports of MPM in the gastrointestinal system, none have reported the combination of the tumors seen in this case. In terms of risk factors, there is minimal overlap between those seen in these cancers, most particularly advanced age. Likewise, apart from individual surgical resection of each of the tumors, there is no overlap in the management of these malignancies, quite possibly making it a challenge. Fortunately, the early diagnosis of this case prompted surgical resection of the tumors, along with selected adjuvant chemotherapy.
PP-03-079
Antibiotic treatment in acute cholangitis geriatric patient with septic shock: Case report and therapeutic insights
Taswin Prawira1 and Dadang Makmun2
1Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia;2Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Central Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Acute cholangitis is a serious infection of the bile ducts, typically caused by an obstruction, often due to gallstones. Immediate treatment and choice of antibiotics, particularly in geriatric patients, are critical as acute cholangitis can lead to severe complications, including sepsis and death.
Case Description: An 83-year-old female with severe abdominal pain and fever was admitted to the emergency department. She had progressively increased jaundice within a week and severe abdominal pain a few hours before admission. Physical examination revealed decreased sensorium, low blood pressure, and icteric sclera. Severe tenderness of the right upper quadrant of the abdomen was also noted. Laboratory examination revealed a haemoglobin level of 10.9 g/dL, WBC count of 26,600 cells/mm³, platelet count of 50,000 cells/mm³, and D-Dimer of 5.07 mg/L. CT scan of whole abdomen revealed multiple choledocholithiasis in the common bile duct, causing obstruction and dilation of intrahepatic and extrahepatic bile ducts. Patient was diagnosed as acute cholangitis. Meropenem was initially administered as an antibiotic, followed by an ERCP procedure with sphincterotomy to remove stones and drain lots of pus. A culture of bile aspirate revealed Pseudomonas aeruginosa and Extended-spectrum beta-lactamase-positive Klebsiella pneumoniae with multi-drug resistance. A novel antibiotic, Ceftazidime-avibactam was administered in accordance with the sensitivity test, and patient's condition was improved significantly. She was discharged in good condition after seven days of administration of antibiotics.
PP-03-080
Eosinophilic Enteritis Disorder presented as chronic abdominal pain- a rare case report
Hendri Priyadi, Muhammad Bestari, Dolvy Girawan, Nenny Agustanti and Eka Nugraha
Division of Gastroentero-hepatology, Department of Internal Medicine, Faculty of Medicine, University of Padjadjaran, Hasan Sadikin General Hospital, Bandung, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Eosinophilic enteritis (EE) is a rare gastrointestinal disorder characterized by eosinophilic infiltration of the intestinal wall, leading to a spectrum of clinical manifestations including abdominal pain, nausea, vomiting, and diarrhea. Diagnosis of EE requires a high index of suspicion, often triggered by peripheral eosinophilia and confirmed through endoscopic biopsies demonstrating eosinophilic infiltrates in the intestinal mucosa. Management strategies predominantly involve corticosteroids to mitigate inflammation, with variable treatment responses observed among patients.
This case report presents a detailed description of a 20-year-old woman admitted to the emergency department with a history of chronic gastrointestinal symptoms over one year. She reported episodes of greenish vomiting, abdominal pain, and chronic diarrhea without fever, weight loss, or rash. Physical examination and initial laboratory tests revealed marked leukocytosis due to eosinophilia, peripheral blood smear confirming hypereosinophilia, and elevated immunoglobulin E levels. Abdominal ultrasound showed findings consistent with gastritis and duodenitis, corroborated by histopathological examination of biopsied tissues demonstrating eosinophilic infiltrates.
Treatment with methylprednisolone 20 mg/day resulted in significant improvement of symptoms. This case underscores the diagnostic challenge posed by EE and highlights the importance of considering this rare entity in the differential diagnosis of chronic gastrointestinal complaints with eosinophilia. Further research is warranted to enhance understanding of EE's pathophysiology and refine therapeutic approaches, particularly in cases resistant to conventional treatments.
This abstract emphasizes the critical role of early recognition and tailored management in optimizing outcomes for patients with EE, underscoring the need for increased clinical awareness and comprehensive diagnostic evaluation in similar clinical presentations.
PP-03-081
An interesting case of a vanishing polyp during colonoscopy due to ileocolic intussusception
Rasyiqatul Raminey and Sumitro Kosasih and Norwani Dewi Basir and Kian Chai Lim and Vui Heng Chong
Gastroenterology Unit, Department of Medicine, RIPAS Hospital, Bandar Seri Begawan, Brunei Darussalam
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Altered bowel habit associated with abdominal pain and weight loss are warning symptoms and requires urgent evaluation given the increasing incidence of colorectal neoplasms including colorectal cancer. A 55-year-old man with a background history of hypertension, dyslipidemia, diet-controlled diabetes mellitus and thalassemia trait presented with a 2 months history of recurrent abdominal pain accompanied with loose stool. He also has weight loss of 3 kg over the 2 months. In between episodes of abdominal pain, his bowel was normal. On admission he had microcytic anemia (Hb 8.5 gm/dL and MCV 53.5). Upper gastrointestinal endoscopy was normal but colonoscopy was abandoned due to poor bowel preparation. A repeat colonoscopy interestingly showed a large elongated polyp seen upon reaching the ascending colon and in the attempt to search for the base of this polyp, the polyp vanished and was not seen on withdrawal. After the polyp vanished, the cecum and the ileocecal valve were seen and noted to be normal. A computed tomography scan confirmed intussusception of an ileal lipomatous polyp. The patient was referred to the surgical department for further management. This case highlights an interesting case of ileocolic intussusception that was reduced during colonoscopy.
PP-03-082
Endoscopic ampullectomy for a well to moderately differentiated ampullary adenocarcinoma: case report
Romielle Grazel Joi Ramos and James Crisfil Fructuoso Montesa
Manila Doctors Hospital, Las Pinas City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Early ampullary cancers present with good prognosis. Pancreaticoduodenectomy has been standard treatment for ampullary cancers, but postoperative complications rate remains high. Therefore, raises a discussion on the role of local ampullectomy for early ampullary cancers.
Case Report: A case of a 72-year-old male, known hypertensive and coronary artery disease (s/p PTCAx1) who presented with 1 week history of jaundice and tea-colored urine. On review of systems, patient also had unintentional weight loss of 10kg within 1 month. On evaluation, patient was noted to have generalized jaundice and icteric sclerae. Abdomen was soft, nondistended and nontender without palmar erythema and spider angiomata. CA 19-9 was noted to be 324x elevated (value: >12000). MRI and MRCP of abdomen revealed presence of a 1.6 cm periampullary soft tissue thickening, with moderate to severe upstream dilatation of the intrahepatic and extrahepatic ducts, and in the pancreatic duct. No liver metastasis and lymphadenopathies were noted. Snare ampullectomy performed via hot snare using Endo Cut Q alternate with soft coagulation. Ampullary mass retrieved measuring 2cm x 1cm x 1.5cm and sent for histopathology. Histopathology revealed ampullary adenocarcinoma. Monthly follow-up showed no recurrence of jaundice and decreasing bilirubin levels. Currently, patient is being treated with adjuvant chemotherapy.
PP-03-083
Post-cholecystectomy hepatic subcapsular biloma: a case report
Romielle Grazel Joi Ramos and James Crisfil Fructuoso Montesa
Manila Doctors Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Hepatic subcapsular biloma is an uncommon yet serious complication after laparoscopic cholecystectomy, marked by bile collecting beneath the liver capsule. Even though it is rare, identifying this condition is essential due to its potential for causing morbidity.
Case report: Case of a 65-year-old female with a medical history of hypertension and dyslipidemia. Patient is a known case of chronic calculous cholecystitis and underwent an uncomplicated laparoscopic cholecystectomy. Postoperatively, she developed intermittent crampy, generalized abdominal pain and constipation. Upon diagnostics, MRI and MRCP of Upper Abdomen revealed moderate-sized loculated fluid collection with some fluid-filled levels and mild surface enhancement of the liver along the right hepatic lobe, leading to the diagnosis of hepatic subcapsular biloma. The biloma was managed successfully with percutaneous drainage. This exhibits a rare complication managed effectively without the need for endoscopic retrograde cholangiopancreatography (ERCP).
PP-03-084
Strongyloidiasis induced duodenal ulcer: an unusual cause of dyspepsia
Kimchhay Ro1 and Khang Chea2
1Calmette Hospital, Phnom Penh, Cambodia;2Ekip medical centre, Phnom Penh, Cambodia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Strongyloidiasis is a common helminth infection in tropical and subtropical regions. Majority of infected patient are asymptomatic or experience dyspepsia. We report a case of strongyloidiasis induced duodenal ulcer in an immunocompetent patient presented with dyspepsia.
Case Description: A 63-year-old non-smoker woman without underlying disease, presented with chronic epigastric pain with postprandial distress syndrome. There were no alarm feature. Initial treatment with proton pump inhibitors (PPI) and prokinetics yielded no improvement. Physical examination and laboratory test were unremarkable. She underwent upper endoscopy which showed a large ulcer surrounded by edematous mucosa at duodenal bulb. Histological examination identified strongyloides stercoralis larva with acute inflammatory cells infiltration and increase eosinophil. Stool examination also confirmed the presence of strongyloides stercoralis ova. The patient was treated with Ivermectin resulting in significant symptomatic improvement. Parasitological stools examination was negative 1 month later.
PP-03-085
Pseudotumor lesion of primary eosinophilic colitis: a rare endoscopic finding
Kimchhay Ro1,2 and Khang Chea1
1Calmette Hospital, Phnom Penh, Cambodia;2Ekip medical centre, Phnom Penh, Cambodia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Eosinophilic colitis is an uncommon disorder in adults with unclear pathogenesis. There is lack of consensus concerning diagnostic criteria. Colonoscopy were normal in more than 70% of case. This current study reported a rare form of primary eosinophilic colitis mimicking primary colon cancer
Case Description: A 59-year-old man without underlying disease, presented 3-months history of abdominal pain with non-bloody diarrhea, and significant weight loss. Laboratory investigation showed elevated eosinophils (30.3%) otherwise normal. An abdominal computed tomography scan suggested a malignant mass at the hepatic flexure. Colonoscopy revealed a circumferential mass-like lesion at the hepatic flexure with multiple erosions surrounded by edematous mucosa, other segments of colon were normal. Histological examination reported hyperplastic glandular component with dense eosinophils. No dysplasia or malignancy seen. Patient was treated with 20mg/day of corticosteroid for 4 weeks then tapered progressively over 4 weeks. Significant clinical improvement within first week of treatment. Complete mucosal healing was confirmed by colonoscopy at 12 weeks follow-up.
Discussion: Endoscopic characteristic of eosinophilic colitis is variable and non-specific. Mucosal lesions usually show nonspecific erythema or ulceration. Pseudotumor lesion is a rare endoscopic finding which could mimic primary colon cancer. Diagnostic establishment rely on the presence of prominent eosinophilic infiltration in colonic mucosa and hypereosinophilia.
PP-03-086
Novel ASD device closure for post esophagectomy leak: two cases with follow up, unique healing
Githma Wimalasena1, Randima Nanayakkara3, Janaka De Silva4, Ishan De Zoysa1,2, Nilesh Fernandopulle1,2 and Dulanja Senanayake1
1Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;3Colombo North Teaching Hospital, Ragama, Sri Lanka;4Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Anastomotic leaks are a notable complication seen in 5% -29% of patients undergoing esophagectomy for malignant indications. Teatment modalities include surgical repair, diversion and exclusion, endoscopic and conservative management. A novel approach involves the utilization of an atrial septal defect (ASD) closure devices, designed for cardiac applications, to seal off leakage sites post-oesophagectomy. Only few case reports of this approach are available in worldwide literature and longterm follow up data are scarce. We present two cases of succesful closure of post esophagectomy anastomotic leaks with an ASD device with 3 years follow up.
Case report: Two male patients, aged 39 and 72 years underwent laparoscopic oesophagectomy for gastroesophageal junction tumour. Both patients were found to have anastomotic leakage within 10 days after surgery. Conventional management approaches failed and ASD closure devices (11mm and 15mm respectively) were successfully placed. Successful leak closure was observed in both cases. Follow up at 6 weeks with CECT revealed no recurrences and the devices were found to be dislodged. At 36 months post-procedure both patients were asymptomatic despite the devices being dislodged.
Discussion: Despite limited use in gastrointestinal interventions, ASD closure devices offer minimally invasive, successful leak closure, potentially superior to conventional methods, providing a promising alternative, especially in refractory cases.
These cases underscores the efficacy of ASD closure devices in managing post-oesophagectomy leaks, highlighting their potential as valuable additions to leak management strategies. Further research into comparative effectiveness is warranted to delineate their optimal role in leak management post-oesophagectomy.
PP-03-087
Submucosal tunneling endoscopic resection (STER) for esophageal submucosal tumors: pioneering surgery in Sri Lanka
Githma Wimalasena1, Nilesh Fernandopulle1,2 and Dulanja Senanayake1
1Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Submucosal tumours (SMTs) are defined as masses originating below the mucosa and protruding into the gastrointestinal lumen. Often asymptomatic, SMTs larger than 3 cm should be resected according to the American Society for gastrointestinal endoscopy (ASGE) guidelines, while smaller non-malignant SMTs can be monitored. Traditional surgical methods are required for deeper lesions, but STER has emerged as a less invasive alternative, combining techniques from endoscopic submucosal dissection, and peroral endoscopic myotomy.
Case report: A 52-year-old female with a 2-year history of dysphagia worsening over 5 months, was found to have a polypoidal growth at the gastroesophageal junction, of which histopathology confirmed a leiomyoma. Endoscopic ultrasound revealed an additional 42mm by 25mm submucosal lump in the mid-esophagus. The patient, classified as ASA class II, underwent STER under general anaesthesia. The procedure involved creating a submucosal tunnel, dissecting the lesion and removing it through the opening, which was closed by endoclips. There was minimal blood loss, no intraoperative complications and the patient recovered without issue, being discharged four days after surgery.
Discussion: SMTs are increasingly detected due to advanced imaging and endoscopic techniques, with a reported incidence of 3%. Histological diagnosis calls for endoscopic ultrasound guided fine needle aspiration. Surgical resection although effective, is invasive with higher morbidity. STER, offering high en bloc resection rates and fewer complications, stands out as a safer alternative, with reduced hospital stays and adverse events.
PP-03-088
Sinistroposition left-sided gallbladder: A rare incidental intraoperative biliary tract variation during laparoscopic cholecystectomy
Githma Wimalasena1, Albert Joseph3 and Duminda Subasinghe2,3
1Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;3University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract
Introduction: The gallbladder is typically located in the hepatic segments IV and V. Left-sided gallbladder, defined as a gallbladder situated to the left of the falciform ligament, is a rare biliary anomaly. It can be, due to situs inversus, or true left-sided gallbladder which is further subdivided into medioposition and sinistroposition. True left-sided gallbladder is exceptionally rare, seen in only 0.04% of cholecystectomy patients. Laparoscopic cholecystectomy in these cases has a higher risk of bile duct injury compared to normally positioned gallbladders.
Case: A 42-year-old obese (BMI=36.5 kg/m²) female presented with a five-month history of recurrent biliary colics. Her liver function tests were normal. Preoperative ultrasound revealed a solitary stone in the gallbladder neck. During laparoscopic cholecystectomy, a left-sided gallbladder (Type III) was found on segment IVB with the right hepatic artery running anterior to the common bile duct. There was an impacted stone in Hartmann’s pouch and two cystic arteries. Despite these challenges, a complete laparoscopic cholecystectomy was performed with a critical view of safety. The operation time was 120 minutes, and histopathology indicated chronic cholecystitis. The patient recovered uneventfully and was well three months post-surgery.
Discussion and conclusions: Left-sided gallbladder is a rare anomaly with a prevalence of 0.1-0.7%. Conventional ultrasound often fails to detect it, surprising surgeons during laparoscopy. Successful removal requires modifications during surgery such as patient repositioning. Left-sided gallbladder, and its associated anormalies increase the risk of bile duct injury. Awareness of these anormalies is crucial for safe laparoscopic cholecystectomy.
PP-03-089
Gastric per-oral Endoscopic Myotomy for Treating Pylorospasm after Sleeve Gastrectomy
Li Wang1,2, Zu-Qiang Liu1, Quan-Lin Li1 and Ping-Hong Zhou1
1Zhongshan Hospital, Fudan University, Shanghai, China;2Endoscopy Center, Shanghai Geriatric Medical Center, Shanghai, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: A 44-year-old man was admitted because of acid reflux and heartburn for three years. The patient had undergone sleeve gastrectomy (SG) and tension-free repair of esophageal hiatal hernia three years and one year before admission, respectively. He was scheduled to undergo gastric per-oral endoscopic myotomy (G-POEM) after gastroscopy and gastric emptying examination, which showed esophagitis, pylorospasm (A), and gastric emptying disturbance.
Case Description: After submucosal injection, a 2-cm mucosal incision was made 5cm above the antrum pylorus (B). After the submucosal tunnel was extended to 3cm below the pylorus, the muscle bundle was completely transected using the hybrid knife (C). After hemostasis with hot biopsy forceps, the mucosal entry on the stomach was closed with clips. After myotomy, the pylorus was remarkably enlarged, and the gastroscope passed smoothly without resistance (D). The patient recovered uneventfully and was discharged on the postoperative day 2. The patient did not experience any discomfort during the two-year postoperative follow-up.
Discussion: Pylorospasm is a rare adverse event after SG. G-POEM after SG was more complicated and riskier than normal due to distortion of the gastric cavity and limited space for the procedure. This case provides the possibility of applying G-POEM to treat pylorospasm after SG.
PP-03-090
Conquering Challenging Common Bile Duct Stones – A Case Report
Louis Wang and Andrew Boon Eu Kwek and James Weiquan Li and Kenneth Weicong Lin and Nicholas Chin Hock Tee and Tiing Leong Ang
Changi General Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Common bile duct (CBD) stones are prevalent and result in severe complications such as acute pancreatitis and cholangitis in untreated patients. Extraction of difficult CBD stones during endoscopic retrograde cholangiopancreatography (ERCP) is challenging.
Case Report: A 43-year-old Malay male with no prior medical history presented with painless obstructive jaundice of three weeks, with serum total bilirubin 511 umol/L, direct bilirubin 461 umol/L, alkaline phosphatase 274 U/L. CT abdomen revealed marked biliary dilatation. MRCP confirmed two 18mm stones with distal CBD narrowing. A decision was made for ERCP with cholangioscopy-guided lithotripsy. Initial attempts using electrohydraulic lithotripsy (EHL) were ineffective in stone fragmentation. Laser lithotripsy (LL) was performed as a rescue procedure with successful stone fragmentation.
Discussion: This case illustrates several characteristics of difficult CBD stones, including larger size (>1.5cm), increased number, hardness, impaction, and presence of distal ductal narrowing. While papillotomy with large-balloon dilation is advocated as first-line therapy for difficult CBD stones, cholangioscopy-assisted lithotripsy is increasingly shown to be more superior for CBD clearance with comparable safety. Although the efficacy of EHL is supposedly comparable to LL, single session fragmentation and ductal clearance rate is significantly higher with LL. Each laser impulse is more focused and of greater intensity than EHL. In calcified stones with failed EHL, LL can be successfully used as rescue therapy. It may be that LL, rather than EHL, should be preferentially used to treat calcified stones. This case highlights the considerations in managing difficult CBD stones, advocating for tailored approaches to enhance therapeutic outcomes.
PP-03-091
A rare cause of occult gastrointestinal (GI) bleeding: Small bowel metastases from malignant melanoma
Ying Wei1, Alyssa and Shin Yee Sim1 and Christopher and Tze Wei Chia2
1Tan Tock Seng Hospital, Singapore, Singapore;2Woodlands Health Campus, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Metastases of primary malignant melanoma to the small bowel occur in nearly 50% of patients but typically remain asymptomatic and undetectable until complications such as abdominal pain, bleeding, or anemia arise. Our case highlights an unusual instance of occult gastrointestinal bleeding due to small bowel metastases in a patient with primary vaginal melanoma.
Case Report: A 48-year-old female with primary vaginal melanoma, diagnosed and resected 15 months prior, presented with severe symptomatic iron deficiency anemia (hemoglobin 5.6 g/L, ferritin 4 ug/L), weight loss, and abdominal bloating. Initial diagnosis and follow-up surveillance 6 months ago showed no distant metastases. A repeat Computed Tomography(CT) scan revealed new small bowel wall thickening and enlarged mesenteric and retroperitoneal lymphadenopathy. Antegrade double-balloon enteroscopy confirmed an ulcerated proximal jejunal mass with associated bleeding. Biopsies identified spindle and epithelioid cells with granular intracytoplasmic melanin pigment, and immunohistochemical stains confirmed recurrent metastatic melanoma. Her hemoglobin levels stabilized with intravenous iron and transfusion, and there was no overt GI bleeding. She was planned for systemic immunotherapy.
Discussion: Although small bowel metastatic melanoma is a rare cause of GI bleeding, it should always be suspected in patients with a history of melanoma presenting with iron deficiency anemia and/or gastrointestinal symptoms. Early investigation in these cases can lead to prompt diagnosis and improved outcomes, especially with the evolving role of immunotherapy.
PP-03-092
Yellow Man: A Case Report About A Patient With Extremely High Bilirubin
Shiran Appuhamy and Chamila Ranawaka
Colombo North Teaching Hospital, Ragama, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: This case report describes a 33-year-old male with a complex medical history including glucose-6-phosphate dehydrogenase (G6PD) deficiency, chronic haemolysis, multiple endocrine disorders and gall stone disease. He was presented with right upper abdominal pain, deep jaundice and fever. He was found to have common bile duct (CBD) stones. Endoscopic Retrograde Cholangiopancreatography (ERCP) successfully identified and cleared multiple CBD stones, resulting in significant clinical improvement.
Case description: A 33-year-old male was admitted with acute right upper abdominal pain, deep jaundice, fever and pallor for five days duration. He is a known patient with G6PD deficiency with chronic haemolysis. He had a past history of cholecystectomy, hypoparathyroidism and hypogonadism. He had consanguineous parents.
Physical examination revealed deep jaundice, pallor, right hypochondrial (RHC) tenderness and splenomegaly. His blood pressure was 90/60 mmHg and pulse rate was 100 bpm. Initial laboratory tests were suggestive of biliary sepsis with cholestasis and ongoing haemolysis. Initial full blood count revealed pancytopenia. Other investigations were as follows;
Total bilirubin - 1205.9μmol/L
Direct bilirubin - 709.1 μmol/L
Indirect bilirubin - 496.8μmol/L
CRP - 23 mg/dL
Ultrasound scan abdomen showed CBD stones causing biliary obstruction with dilated CBD. He underwent urgent ERCP and three large stones were extracted.
Discussion: Our patient already had chronic haemolytic anaemia due to G6DP deficiency and developed CBD stones over time. CBD stones were complicated with cholangitis, and it triggered acute haemolysis. Both acute haemolysis and cholestasis due to CBD obstruction could the possible explanation for the unusually high indirect and direct bilirubin levels.
PP-03-093
Endoscopic prevention of retrograde biliary drainage stent insertion for post-endoscopic retrograde cholangiopancreatography cholangitis
Yeong Heon Han, Eui Joo Kim, Huapyong Kang and Yeon Suk Kim
Gachon University Gil Medical Center, Incheon, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Antibiotic prophylaxis may reduce the risk of infectious complications following endoscopic retrograde cholangiopancreatography (ERCP). However, the efficacy of endoscopic prophylactic measures for preventing post-ERCP cholangitis remains a subject of debate.
Methods: In 2023, patients with choledocholithiasis who underwent ERCP were retrospectively reviewed at a single tertiary medical center. Data collected included patient demographics, diagnostic information, laboratory results, and ERCP-related details. Patients were classified as having post-ERCP cholangitis if they exhibited jaundice, fever, abdominal pain, and/or purulent drainage during a repeat ERCP. Bile duct stones were removed from all patients, and the clinical effect of a 5 Fr endoscopic retrograde biliary drainage (ERBD) stent after successful biliary stone removal was analyzed.
Results: Data from 261 patients with choledocholithiasis were included. The mean age of the patients was 70.1 years, with 47.1% being women. All patients received prophylactic antibiotics prior to ERCP. Univariate analysis indicated that the 5 Fr ERBD stent was effective in reducing total bilirubin levels 4 hours after ERCP (P<0.023); however, there was no statistically significant effect on total bilirubin levels 24 hours after ERCP (P=0.105). There were 4 patients (1.3%) with post-ERCP cholangitis, and no differences were observed between patients who had the 5 Fr ERBD stent inserted and those who did not (1.7% VS 1.3%, P>0.999).
Conclusion: In patients whose biliary stones have been completely removed and who are administered prophylactic antibiotics, the insertion of a 5 Fr ERBD stent does not confer additional prophylactic efficacy in reducing post-ERCP cholangitis.
PP-03-094
Diagnostic and Therapeutic Utility of SpyGlass Cholangioscopy in Biliary tract diseases: A single center experience
Leah Anne Legaspi and Evan Ong
Metropolitan Medical Center, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: Biliopancreatic ductal lesions pose diagnostic and therapeutic dilemma due to limited endoscopic access. SpyGlass cholangioscopy has provided an avenue for investigating these lesions through direct visualization. We aimed to review and summarize our single center experience.
Materials and Methods: Fifty-nine (59) patients underwent SpyGlass cholangioscopy at our center between June 2022 to December 2023. Demographics, indications, technical and clinical success, difficulties, complications, and outcome were determined and assessed.
Results: Fifty-nine patients underwent 69 SpyGlass cholangioscopies. The average age is 58 years (55±16), with 54% males. Diagnostic indication includes – ductal stricture in 41 cases (59%), ductal clearance documentation in 22 (32%), adjunct to SEMS insertion in 5 (6%), Holmium lithotripsy in 2 (2%) and intraductal fractured guidewire tip removal in 1 (1%). Majority 23 (60%) of strictures were hilar and common hepatic duct in location. Intraductal biopsy was employed in 32 strictures. Sixty-nine percent (25 biopsies) had benign histopathology while premalignant/malignant cases were seen in 31% (11 biopsies). The per-oral route was used in 65 cases (94%) and percutaneously in 4 cases (6%). Both technical and clinical success was achieved in 69 procedures (100%) and 40 procedures (100%) respectively. Difficulties are mainly inadequate visualization. Complication rate was 9% with mild to moderate severity, including cholangitis, pancreatitis, pain and malaise, all managed medically.
Conclusion: SpyGlass cholangioscopy is a promising and a potentially useful tool in diagnosis and treatment of pancreaticobiliary diseases through direct visualization and targeted therapeutics. It has a high technical and clinical success with low periprocedural complication.
PP-03-095
A novel design of sphincterotome for patients with surgically altered gastric anatomy
Mai Sorachi and Toshinobu Izumi
NHO Himeji Medical Center, Himeji City, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: It is difficult to control the orientation of a sphincterotome in patients with surgically altered gastric anatomy. Sphincterotomes available in this setting are rarely directed as intended. We designed sphincterotome to obtain the correct axis of the upside-down papilla. We report experiences about the new device.
Materials and Methods: Sphincterotomy was performed in 10 cases with altered gastric anatomy (4 with Roux-en-Y reconstruction and 6 with Billroth II) from August 2021 to May 2024. Double-balloon endoscopes were used for Roux-en-Y and side-viewing duodenoscopes for Billroth II except a case using a double-balloon endoscope. We adopted a push-type sphincterotome. Its tip was curled by hand with the cutting wire allowed to run along outside of the bowed tip (Figure 1).
Results: The new sphincterotomes steadily pointed to the proper alignment of the inverse papilla, resulting in completion of the procedure in all the patients. Side-viewing duodenoscopes tended to offer better views of the cutting site than forward-viewing balloon endoscopes. No significant complications including bleeding, pancreatitis and perforation were not found.
Conclusion: When previous gastric surgery turns the anatomy of the papilla ‘upside down’, sphincterotomy could be performed steadily by using the new instrument.
PP-03-096
Metal stent versus plastic stent in endoscopic ultrasound-guided hepaticogastrostomy for unresectable malignant biliary obstruction
Daiki Yamashige, Susumu Hijioka and Takuji Okusaka
National Cancer Center Hospital, Chuo-ku, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: Whether metal stents (MSs), which are commonly used, or plastic stents (PSs) yield better outcomes for malignant biliary obstruction (MBO) in endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) is controversial. Thus, we aimed to compare outcomes of initial EUS-HGS performed with MSs or PSs.
Methods: In this single-center retrospective study, we included patients (MS/PS groups: n=158/77) with unresectable MBO and performed multivariable analysis. The landmark date was defined as day 100 and used to evaluate the time to recurrent biliary obstruction (TRBO).
Results: The clinical success rate was similar in both the groups. The mean total bilirubin percentage decrease at week 2 was significantly higher in the MS group than in the PS group (-45.5% vs. -27.3%, P=0.03). Median TRBO was significantly different between the MS and PS groups (183 and 92 days, respectively, P=0.048). TRBO within 100 days was comparable in both the groups but was significantly shorter only after 100 days in the PS group (adjusted hazard ratio [HR] 14.7, P<0.001). Adverse events (AEs) were significantly more common in the MS group than in PS group (24.7% vs. 9.1%, P=0.005), although AE risk was higher with PS in the cholangitis subgroup (Pinteraction=0.02). After endoscopic re-intervention (ERI), TRBO tended to be longer with revision PS (HR 0.64, P=0.26).
Conclusion: Although MS provides early improvement of jaundice and long stent patency, PS provides a better safety profile, comparable stent patency until 100 days, and favorable stent patency after ERI. PS can also be an adequate and optimal palliation method in EUS-HGS.
PP-03-097
Efficacy and tolarability of the split and the standard bowel preparation regime, for colonoscopy
1Teaching Hospital Peradeniya, Kandy, Sri Lanka;2University of Peradeniya, Kandy, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Optimal bowel preparation is vital for the safety and efficacy of colonoscopy. The efficacy and tolerability of bowel preparation remains a problem worldwide. Most centers in Sri Lanka use 4L of Polyethylene glycol (PEG) interspersed with similar amount of clear liquids as a single dose. Some centers use split dose regime where 2L of PEG is prescribed in the evening prior to the study and the other 2L is prescribed next morning. To compare the efficacy and the tolerability of the split dose regime in comparison to the single dose regimen.
Materials and Methods: An interventional study design was used. Patients awaiting colonoscopy were assigned in to the two arms.Those who had previous large bowel resections, diagnosed patients with bowel motility disorders were excluded. They were admitted day prior to procedure and administered the bowel preparation accordingly. One of the investigators inquired regarding compliance and the tolerability. During the procedure, the colonoscopist graded the bowel preparation in each colonic segment according to a validated scale, Boston Bowel Preparation Score (BBPS)
Results: Fifty-four patients were in each group. Thirty-four out of them were females. Mann-Whitney U test was used to compare the distributions. Split dose regime showed a statistically significant difference in BBPS in all 3 segments of the colon and tolerability compared to continous regime (right: p=0.005, transverse: p<0.001, left: p<0.001)
Conclusions: The split dose bowel preparation regime is superior to continuous regime in its efficacy of bowel preparation in all colonic segments and tolerability as well.
PP-03-098
Diagnostic value of artificial intelligence computer assisted diagnosis (cad eye function) for colorectal polyps
Hendra Asputra, Achmad Fauzi, Hasan Maulahela and Cleopas Martin Rumende
Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To assess diagnostic value of AI CAD eye function for colorectal polyps
Material & Methods: This study used a crosssectional design and was conducted at the RSCM Gastrointestinal Endoscopi Center in January-May 2024 on adult patients with of colorectal polyps.
Result: A total of 60 patients with 100 polyps were involved in this study. The median age was 61.5 (20–76) years with 50% males and 50% females, the majority was Javanese (46.7%), 12% obese, 30% had IBD, 20% had diabetes, 13.3% had a family history of polyps, and 10% had a family history of colorectal cancer. The majority of polyps are 1-5 mm in size (56%),in the rectosigmoid (51%) and have a sessile morphology (75%). Based on the results of the examination, it was found that the AI CAD eye function examination had a sensitivity 79.17%, specificity 75%, PPV 89.06%, NPV 58.33% and accuracy of 78%. In polyps with dimminutive size, sensitivity 86.27%, specificity 60.00%, PPV 95.65%, NPV 30.00% and accuracy 83.93% were obtained. Meanwhile, in polyps with a nondimminutive size, sensitivity 61.90%, specificity 78.26%, PPV 72.22%, NPV 69.23%, and accuracy 70.45% were obtained. In sessile polyps, sensitivity was 81.54%, specificity 50.00%, PPV 91.38%, NPV 29.41% and accuracy 77.33%. Meanwhile, in non-sessile polyps, sensitivity was 57.14%, specificity 88.89%, PPV 66.67%. NPV 84.21%, and accuracy 80.00%
Conclusion: AI CAD eye function examination has a high sensitivity value in dimmunitive and sessile polyps and high specificity in nondimmunitive and non sessile polyps.
Keywords: AI, CAD eye function, colorectal polyps
PP-03-099
Characteristics of colorectal polyp patients at the gastrointestinal endoscopy center RSCM Jakarta period January-May 2024
Hendra Asputra
Faculty Of Medicinie University Of Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To assess the demographic features, risk factors, and characteristics of colorectal polyps at PESC Cipto Mangunkusumo Hospital from January to May 2024.
Material & Methods: This descriptive-analytic study was conducted at the PESC RSCM from January to May 2024 on adult patients with colorectal polyps.
Result: A total of 60 patients with 100 polyps were included in this study. Median (IQR) age was 61.5 years (20-76 years) most are within the range of 60-69 years (38.3%), gender are equal between men and women, which were 50% and 50%, the majority are of Javanese ethnicity (46.7%), followed by Batak and Chinese ethnicity each by 15%, a total of 12% of patients were obese, and 30% patients had a history of IBD, 20% had a history of diabetes mellitus, and 13.3% had a family history of polyps, and only 10% had a family history of colorectal cancer. The majority of polyps were 1-5 mm in size (56%), most located in the rectosigmoid (51%) and the majority of sessile morphology (75%).
Conclusion: The majority were rectosigmoid sessile polyps, age 60-69, Javanese ethnicity, not obese, no history of IBD, no history of diabetes mellitus, no family history of polyps and colorectal cancer. The appearance of polyps in this study was most likely spontaneous.
Computer-Aided Detection Versus Conventional Colonoscopy For Proximal Colon Polyps: Single Centre Randomized Tandem Colonoscopy Study
Nur Nadia Azman, Rafiz Abdul Rani, Thevaraajan Jayaraman and Muhammad Ilham Abdul Hafidz
Universiti Teknologi MARA (UiTM), Sungai Buloh, Malaysia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: Screening colonoscopy has had limited impact on improving outcomes for right-sided colorectal cancers. Computer-aided detection (CADe) was shown to improve detection of colorectal polyps. We aimed to evaluate adenoma detection rate (ADR) in the right colon using CADe vs conventional white light imaging (WLI).
Methodology: This was a prospective randomized, tandem colonoscopy study conducted at Universiti Teknologi MARA. Screening colonoscopy patients aged 50-70 years were enrolled and randomly assigned to either first undergo CADe (CAD EYE®, Fujifilm Co, Japan) or WLI from caecum till splenic flexure, followed immediately by the other procedure in tandem and by a different endoscopist blinded from the first procedure.
Results: Fourty four patients met the inclusion criteria with mean age of 63.6 ±6.2 years, majority female (56.8%) and ethnicly mainly Malay (68.2%). 52.3% and 56.8% patients scored Boston Bowel Prep Score of 3 at ascending colon and transverse colon respectively. The median (IQR) withdrawal time for CADe in the right colon was higher 8 (6,10) mins vs 7 (5,10) minutes. CADe had a higher ADR (27.3% vs. 20.5%, p <0.001) with higher total number of polyps detected (85 vs 72, p<0.001). CADe had higher Polyp Detection Rate (PDR 72.7% vs. 56.8%, p <0.0054). All polyps were <1cm. CADe detected more protruded polyps (Paris 0-Ip, 0-Is) (31vs 28, p<0.001) and flat polyps (Paris 0-IIa) (47 vs 37, p<0.001).
Conclusion: CADe had a higher ADR and a higher number of total polyps detected in the right colon compared to conventional WLI colonoscopy.
PP-03-101
Topical Formalin Instillation vs. Argon Plasma Coagulation in Chronic Radiation Proctopathy: A Meta-analysis
Jan Bendric Borbe and Eric Yasay
Philippine General Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Radiation proctopathy is a recognized complication of radiation therapy of pelvic tumors which often presents as rectal bleeding. While argon plasma coagulation (APC) is presently considered the treatment of choice for chronic radiation proctopathy (CRP), its use may be limited by availability especially in developing countries. The objective of this study is to assess the efficacy of topical formalin instillation compared to argon plasma coagulation in the management of CRP.
Materials and Methods: Various electronic databases were searched from inception to December 2023. Randomized controlled trials comparing the effect of argon plasma coagulation and topical formalin instillation in the treatment of CRP were included in the study. The analysis performed using Revman version 5.4.
Results: A total of 2 studies with a total of 57 patients were included in the quantitative analysis. Pooled analysis showed no statistically significant difference in the pre- and post-treatment change in hemoglobin between the formalin and the APC group 0.53 g/L (p = 0.11). In terms of the number of sessions to achieve the desired therapeutic endpoint, pooled analysis of the studies showed no statistical difference between the two groups with a mean difference of 0.06 days (p = 0.87) with no significant heterogeneity (I² 0%).
Conclusion: The results suggest that topical formalin and APC are both effective treatments for chronic radiation proctitis with comparable rates of decreasing rectal bleeding as evidenced by improvement in post-treatment hemoglobin values. Similarly, the number of sessions required to achieve therapeutic success are comparable
PP-03-102
Lactulose vs. Polyethylene Glycol as Bowel Preparation Agent for Colonoscopy: A Metaanalysis
Jan Bendric Borbe, Eric Yasay, Anton Elepano and Glen Anarna
Philippine General Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Lactulose is a non-absorbable disaccharide listed under the World Health Organization’s essential medicines. We aimed to summarize the evidence on the efficacy and tolerability of lactulose as an alternative bowel cleansing regimen to polyethylene glycol (PEG) for colonoscopy.
Materials and methods: Literature search in databases and registers was done in April 2023. Included studies were randomized trials involving adult patients which compared lactulose to PEG for bowel preparation regardless of the indication for colonoscopy. Outcomes of interest were the quality of bowel preparation measured using ordinal scales, polyp detection, palatability of solution, and adverse events.
Results: A total of four studies which comprised 536 patients were included in the analysis. Risk of bias is low in terms of participant selection, but there were issues on participant blinding and heterogeneity on the preparation of the interventions.
In all four studies, the quality of bowel cleanliness with lactulose was non-inferior to that of PEG. Odds of polyp detection favored lactulose over PEG (odds ratio 3.10; 95% confidence interval, 1.42 to 6.74) in one study. Meanwhile, palatability was also better in the lactulose group (odds ratio 0.10; 95% confidence interval 0.03 to 0.39) based on three studies. Usual adverse effects with use of lactulose were nausea, vomiting, abdominal pain, bloatedness, and dizziness.
Conclusion: This systematic review showed that lactulose oral solution is both efficacious and well-tolerated for pre-colonoscopy bowel preparation. In settings where it is readily accessible, it may be a viable substitute bowel preparation agent to PEG for colonoscopy.
PP-03-103
Long-term oncological outcome of colonic stenting as bridge-to-surgery in malignant large bowel obstruction
Lee Yoong Elizabeth Chen, Jun Ming Samuel Lim, Andrew Boon Eu Kwek, Mun Wang Lai, James Weiquan Li, Kenneth Weicong Lin and Tiing Leong Ang
Changi General Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Self-expandable metal stents (SEMS) as bridge-to-surgery (BTS) for malignant large-bowel obstruction (MBO) has increased in recent years, with high success-rates. However, the long-term oncological impact of colonic stenting has received mixed reviews, with concerns regarding metastatic recurrence-risk. We performed an audit in a tertiary centre examining outcomes over a 10-year period following SEMS insertion as BTS.
Materials and methods: Data of patients who underwent SEMS insertion for MBO between September 2013-December 2023 were reviewed. Overall survival, metastases-free survival, metastatic occurrence, and association with AJCC staging and adjuvant chemotherapy were analysed.
Results: Of 132 patients, we excluded 31 who had metastases at diagnosis and five who were lost to follow-up. Of the remaining 96, overall survival and disease-free survival were 90.9% and 66.2% respectively. Mean overall survival was 86.8 months (95% CI [75.1-98.6]), mean metastases-free survival 95.6 months (95% CI [88.7-102.5]). 24 patients (31%) developed distant metastases: 20 (83.3%) were AJCC stage 3, 11 (45.8%) completed adjuvant chemotherapy, 3 (54.1%) declined or had attenuated doses. Using Kaplan-Meier estimates, the association between lack of completion of adjuvant chemotherapy and metastatic occurrence was insignificant (P-value 0.114). Metastatic recurrence was positively associated with higher AJCC staging (P-value 0.037).
Conclusion: Rate of occurrence of distant metastases was similar to prior reported literature. Majority who developed metastases were AJCC stage 3 who declined or had attenuated adjuvant chemotherapy. The data suggests that colonic stenting as BTS does not worsen long-term oncological outcome. Metastatic recurrence was related to disease-stage and lack of effective adjuvant chemotherapy.
PP-03-104
Use Of Gel Immersion Technique In Colonic Diverticular Bleeding
Ronell Lee, Patricia Te and Willy Alba
Chinese General Hospital And Medical Center, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives
• To demonstrate the consistency of the gel
• To compare the visibility of water from the gel once mixed with fresh blood
• Demonstrate the effectiveness of gel immersion technique in active colonic diverticular bleeding
Materials and methods
Materials: Viscoclear gel #1, 50cc Syringe filled with fresh blood #1, Liter Glass bottles #2, Water
Methods: Two different 1.0 Liter glass bottles were filled with same amount of water in one bottle and viscoclear in another bottle. Fresh blood in a 50cc syringe was poured on both bottles consecutively. The mixtures were observed up to 1 hour.
Clinical: An 87 year old male with a Lower Gastrointestinal bleeding from an ascending colon diverticulosis underwent colonoscopy. Gel was flushed to the bleeding site.
Results: Water immediately mixed with blood upon contact. Gel appeared to hold and prevent blood from scattering throughout the gel medium. After 1 hour, water appeared to mix thoroughly with blood while the blood in the gel did not scatter.
Clinical: The bleeding lesion was easily identified because the bleeding was slowly oozing from the site and did not scatter. A hemoclip was deployed on the bleeding site. Hemostasis was achieved afterwards.
Conclusion
• The gel immersion technique is an effective method of improving endoscopic visualization during active colonic diverticular bleeding
PP-03-105
Improving Colorectal Polyp Size Measurement with Artificial Intelligence: Prospective, Preliminary Comparison of Accuracy
1Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, China;2Department of Colorectal Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Accurate polyp size measurements are crucial for risk stratification, selection of polypectomy techniques, and follow-up interval assignments. This study aimed to compare the accuracy of a deep learning-based system (EndoDASS) to visual estimation, non-calibrated tools (biopsy forceps or snares), and calibrated forceps in measuring polyp size during real-time colonoscopies.
Materials and methods: Visual estimation, non-calibrated tools, calibrated forceps-based estimation, and EndoDASS were compared using a 3D-printed colorectal model with 10 polyps of defined sizes. Ten endoscopists estimated polyp sizes visually, using non-calibrated tools, and calibrated tools. Repeated measurements were performed one week apart to evaluate measurement consistency. Additionally, measurements of 24 colorectal polyps during clinical colonoscopies were compared. The primary outcome was relative accuracy in polyp size measurement.
Results: EndoDASS exhibited a relative size measurement accuracy of 96.3%, significantly surpassing that of visual estimation (75.7%, P<0.001), non-calibrated tools (86.4%, P<0.001), and calibrated forceps (80.8%, P<0.001) in the 3D-printed model. EndoDASS demonstrated superior measurement consistency, with an intraclass correlation coefficient of 0.999 for two repeated measurements, surpassing visual (0.577, P<0.001), non-calibrated tools (0.807, P<0.001), and calibrated forceps (0.774, P<0.001). During real-time colonoscopies, EndoDASS achieved a significantly higher relative accuracy (86.7%), compared to visual (54.1%, P<0.001) and non-calibrated tools (69.2%, P=0.008). EndoDASS demonstrated a significantly higher percentage of accurate size measurements within 25% of the true size (95.8%), compared to visual estimation (45.8%, P<0.001) and non-calibrated tools (54.2%, P<0.001).
Conclusion: The novel deep learning-based system, EndoDASS, demonstrated significantly higher accuracy in measuring colorectal polyp size compared to other common sizing methods.
PP-03-106
Results of colonoscopic resection of large ( ≥ 2 cm) pedunculated polyps with endoloop-assisting device
Thuy Tran Ngoc1 and Tuyet Le Thi Anh2
1Thai Nguyen Medical College, Thai Nguyen, Viet Nam;2108 Military Central Hospital, Ha Noi, Viet Nam
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate efficacy and safety of cutting large pedunculated colorectal polyps using an Endoloop-assisting device.
Materials and Methods: Prospective study on 88 patients with pedunculated colorectal polyps ≥ 2 cm in size. These polyps were ligated with an Endoloop and cut using an electric snare through flexible endoscopy in 108 Military Hospital, Ha Noi, Viet Nam, from April 2023 to June 2024.
Results: The average age of the study patient group was 59.8 ± 14.4 years old. The male/female ratio was 2.4/1. The number of polyps ≥ 2 cm was 88 polyps in 88 patients. The main location was in the left colon (48.9%). The average size of polyps was 2.5 ± 0.7 cm. The average length of the polyp stalk was 1.2 ± 0.6 cm. The average polyp stalk diameter was 1.2 ± 0.3 cm. Successful polyp removal results were 100%. The average polyp removal time was 21.2 ± 8.8 minutes. Post-resection complications: secondary bleeding rate was 2.4% due to enlarged blood vessels feeding the polyp, additional bleeding was stopped with clips, post-polyp removal abdominal pain rate was 20%. The rate of patients staying in hospital < 24 hours after polyp removal was 84.0%.
Conclusion: Removing pedunculated colorectal polyps ≥ 2 cm with the support of Endoloop is an effective, safe method with few serious complications.
PP-03-107
Lemmel’s syndrome as uncommon cause of obstructive jaundice : a rare case report
Iqbal Habibie1, Vesri Yoga2, Arnelis2 and Saptino Miro2
1Faculty of Medicine, Andalas University, Padang, Indonesia;2Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine, Andalas University, Padang, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Lemmel’s syndrome is obstructive jaundice without choledocholithiasis or pancreaticbilliary tumors. This syndrome was first reported in 1934 by Lemmel, who described the presence of periampullary duodenal diverticulum (PAD). Periampullary duodenal diverticulum (PAD) is usually asymptomatic, and is found incidentally in 22% of the population, only 5-10% of patients show complications. This syndrome of obstructive jaundice of duodenal diverticulum is called Lemmel’s syndrome.
Case Description: A 64-year-old woman presents to the emergency room with jaundice and abdominal pain. Abdominal ultrasound showed dilatation of the central intrahepatic bile duct without a demonstrable cause of obstruction. An ERCP decision is taken, in which to confirm the diagnosis.
Discussion: Endoscopic retrograde cholangiopancreatography (ERCP) showed giant diverticula in the second part of the duodenum. Cholangiography showed dilatation of the CBD without a filling defect, indicating the absence of stones or tumors in the bile duct. After stent placement, all symptoms improved. Recognizing this condition is important because misdiagnosis can lead to mistreatment and delays in therapy. Lemmel’s syndrome should always be included in the differential diagnosis of obstructive jaundice if PAD is present.
Keywords: Lemmel’s syndrome, PAD, ERCP
PP-03-108
Hybrid Peripheral Nerve Sheath Tumor Detected by Endoscopic Full-Thickness Resection for Gastric Submucosal Tumors
Mai Fukuda
Department Of Endoscopythe Jikei University School Of Medicine, Tokyo, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Case Description: Hybrid peripheral nerve sheath tumors are exceedingly rare, especially those located in the stomach. Here, we present a case of an enlarged gastric submucosal tumor (SMT) treated with endoscopic full-thickness resection (EFTR), leading to a pathology diagnosis of a hybrid peripheral nerve sheath tumor. A 50-year-old woman presented with a 10-mm SMT on the posterior wall of the upper gastric curvature. The tumor, contiguous with the fourth layer of the gastric wall, appeared as a low-homoechoic area on endoscopic ultrasound (EUS). Following a boring biopsy, the tumor tested positive for S100 and SOX10 protein immunostaining, initially diagnosed as a granular cell tumor. However, six months later, the tumor had grown to 15 mm in size, prompting endoscopic resection. Due to the tumor's involvement of the muscle layer, the procedure was performed under general anesthesia, with the possibility of surgical conversion. Despite the challenge posed by the large, kyphotic lesion, resection was successfully achieved across all layers contiguous to the muscle layer. Post-resection, the ulcer, including the perforated area, was closed with clips. The patient's postoperative course was favorable, and she was discharged on the 7th day. Pathology results confirmed the diagnosis of hybrid peripheral nerve sheath tumor. EFTR was not only effective as a treatment but also served as a diagnostic method. This case suggests that EFTR may be an effective diagnostic and therapeutic option for small subepithelial lesions (SELs).
PP-03-109
Endoscopic submucosal dissection after chemoradiotherapy for locally advanced rectal cancer
Chun-Sheng Shen1,2,3,4, Ping-Ju Wu2,3,5 and Ching-Chun Li1,2,3
1Kaohsiung Municipal Siaogang Hospital, Kaohsiung City, Taiwan;2Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan;3Kaohsiung Medical University, Kaohsiung City, Taiwan;4Kaohsiung Medical University Gangshan Hospital, Kaohsiung City, Taiwan;5Graduate Institute of Clinical Medicine Kaohsiung Medical University, Kaohsiung City, Taiwan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: We report an elderly case with locally advanced rectal cancer underwent chemoradiotherapy then resected by endoscopic submucosal dissection.
Case Description: An 85-year-old male with rectal cancer, 2cm from anal verge, adenocarcinoma, grade 2, cT2N0M0, stage I, who underwent 3 cycles of chemotherapy with FOLFOX6 during October 2023 till November 2023 and radiotherapy (tomotherapy) during October 2023 till December 2023, total dose 5000cGy/25fr. He later took oral form Xeloda from December 2023 till May 2024. Follow-up of colonoscopy revealed regression of the rectal cancer in April 2024. As he has coronary artery disease with stenting in 2020 and heart failure, he is not a surgical candidate. After multiple-discipline team discussion, he underwent endoscopic submucosal dissection (ESD) with only local anesthesia. The ESD expanded 40 minutes, the pathology of specimen consisted of one tissue fragment measuring 4.2 x 2.0 cm in size with only adenomatous hyperplasia and no residual adenocarcinoma. There was no intra-procedure and delayed complication. He discharged safely 3 days later and has been followed up in the out-patient clinic recently.
Discussion: ESD for locally advanced rectal cancer is an option as local excision rather than wait-and see strategies. For elderly patient who is vulnerable to traditional surgery, this procedure might be the last resort for providing pathology result of proper treatment and might be a curative intervention.
PP-03-110
Diagnostic Dilemma of Biliopancreatic Contrast-Enhanced Harmonic Endoscopic Ultrasonography
Takahiro Abe and Masayuki Kato and Nana Shimamoto and Keisuke Kanazawa and Masafumi Chiba and Kimio Isshi and Kazuki Sumiyama
The Jikei University School Of Medicine, Tokyo, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The effectiveness of contrast-enhanced harmonic endoscopic ultrasonography (CH-EUS) in the biliopancreatic region is limited because it relies heavily on the endoscopist's skill. This study aimed to validate CH-EUS's efficacy in clinical practice.
Methods: From January 2018 to March 2019, 301 patients who underwent CH-EUS were enrolled. The diagnostic performance of CH-EUS was compared with dynamic computed tomography (CT), magnetic resonance imaging (MRI), and all combined methods using a Bonferroni correction. Multiple logistic regression was used to identify diseases where CH-EUS matched the final diagnosis.
Results: No significant differences in diagnostic performance were found among dynamic CT, MRI, and CH-EUS (P = 1.00). However, combining all methods significantly improved diagnostic performance (P < 0.001). Only intraductal papillary mucinous neoplasm (IPMN) showed significant agreement with the final diagnosis (P = 0.006).
Conclusions: CH-EUS can diagnose IPMN in clinical practice. For other biliopancreatic diseases, a comprehensive diagnosis using multiple methods is needed, rather than relying on CH-EUS alone.
This research has been published in the (Diagnostics 2022).
PP-03-111
Endoscopic Ultrasound Guided Gastroduodenal Artery Embolization- A Case of Refractory Bleeding Ampullary Carcinoma
Deborah Chia Hsin Chew1,4, James Emmanuel2, Wing Hang Woo and Nisha Puvanendran1 and Tiong See Lee3
1National University of Malaysia Medical Centre, Cheras, Malaysia;2Queen Elizabeth Hospital, Kota Kinabalu, Malaysia;3Selayang Hospital, Batu Caves, Malaysia;4GUT Research Group, Cheras, Malaysia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
A 62-year-old lady with a background of ampullary cancer with liver metastasis post hepaticogastrostomy due to failed ERCP and palliative chemotherapy presented to the emergency unit with malena. She was in hypovolemic shock with a blood pressure of 85/46 mmHg and a heart rate of 112 beats per minute. Haemoglobin level was 3.5 g/dL. Oesophagogastroduodenoscopy performed revealed a bleeding ampullary tumour. APC temporarily achieved hemostasis, however, she developed a recurrence of Upper GI bleeding the following day. Computed tomography angiography of the abdomen showed dense material within the duodenum in keeping with blood clots however there was no active bleeding identified. Another session of APC was performed unsuccessfully. An EUS was performed immediately to localize the feeding vessel. The proximal aspect of the Gastroduodenal artery was identified from D1 of duodenum via EUS guided Color-doppler and was punctured with a 22G needle followed by injection of 0.5cc histoacryl glue, monomeric n-butyl-2-cyanoacrylate into the vessel. No further Doppler signal was noted from the GDA post embolization and haemostasis was achieved evidenced on the endoscopic view of the echoendoscope. Patient tolerated the procedure well with no evidence of complications. There were no further episodes of rebleed following the embolization procedure and the patient was discharged after 2 days. EUS-guided injection of sclerotherapy offers a less invasive yet effective approach for achieving haemostasis in cases of refractory bleeding AC. This method can be considered as a potential alternative when standard treatments have failed or are not suitable for the patient.
PP-03-112
Early Chronic Pancreatitis Findings by Endoscopic Ultrasonography (EUS) in Asymptomatic Patients with Pancreas Divisum
Masatsugu Nagahama, Yuichi Takano, Fumitaka Niiya, Tetsushi Azami, Jun Noda and Naoki Tamai
Division of gastroenterology, Showa University Fujigaoka Hospital, Yokohama, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Pancreatic divisum (PD) is a congenital malformation associated with chronic and recurrent acute pancreatitis. Although PD often presents asymptomatically, the extent to which early chronic pancreatitis (ECP) changes occur in asymptomatic patients with PD remains unclear. This study aimed to evaluate endoscopic ultrasonography (EUS) findings indicative of ECP in asymptomatic patients with PD and investigate the relationship between these findings and background factors, such as age, sex, main pancreatic duct diameter, and alcohol intake.
Materials and Methods: We retrospectively analyzed 17 asymptomatic patients diagnosed with PD at the Showa University Fujigaoka Hospital between January 2016 and May 2024. EUS was used to assess the pancreatic parenchyma and ductal features, and the findings were classified according to the Rosemont Classification (RC) and the Japan Pancreas Society (JPS) 2019 criteria for ECP (JDCECP2019). Statistical analyses were performed to examine the association between EUS findings and patient background factors.
Results: EUS findings of ECP were observed in eight of 17 asymptomatic patients with PD (47%) according to both the RC and JDCECP2019 criteria. Hyperechoic foci without shadowing or strands were the most common findings, present in 82% of patients. No significant associations were found between EUS findings and background factors of age, sex, main pancreatic duct diameter, or alcohol intake.
Conclusions: A considerable proportion of asymptomatic patients with PD exhibited EUS findings suggestive of early chronic pancreatitis. These findings indicate that early changes in chronic pancreatitis may be accompanied by asymptomatic patients with PD.
PP-03-113
Endoscopic ultrasound (EUS) guided diagnosis of pancreatic lesions – Single center Experience in Sri Lanka
1Department of Surgery, Faculty Of Medicine, University Of Colombo, Colombo, Sri Lanka;2University Surgical Unit, National Hospital of Sri Lanka, Colombo, Sri Lanka;3Division of HPB/GI Surgery, Department of Surgery, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka;4Colombo North Teaching Hospital, Ragama, Sri Lanka;5Colombo South Teaching Hospital, Kalubowila, Sri Lanka;6Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: EUS-FNA allows histopathological diagnoses of pancreatic tumours to be made when radiological imaging is inconclusive or when histological conformation is required before neoadjuvant therapy for inoperable pancreatic cancers. Previously, these patients were subjected to surgery or chemotherapy solely based on radiology and tumour markers which have their own inherent risks. We aimed to assess the diagnostic accuracy of EUS-FNAB in patients with suspected pancreatic tumours where histological confirmation was required.
Material and Methods: A total of 297 patients with suspected pancreatic masses were referred for EUS to University Surgical Unit, National Hospital of Sri Lanka ,where EUS-FNAB was done.
Results: The mean age of the population was 54.91 years (SD – 15.07 years) and 156 (52.5%) were males. A majority of the lesions (161,54.2%) were solid and found in the head (57.5%). Complications included pancreatitis and infection in 2 patients each (0.67%) which were managed medically. 115 (61.2%) were malignanct lesions, 37 (19.7%) inflammatory and 36 (19.1%) benign. Out of the malignant lesions, 68 (59.1%) were adenocarcinomas and 19 (16.5%) neuroendocrine tumors. Solid masses were significantly associated with malignancy (p<0.001). Adenocarcinomas were more likely to be seen in males (p=0.013), and in the head of the pancreas (p=0.009).
Conclusion: In an era where personalized medicine is advocated, EUS-FNAB of pancreatic neoplasms allows effective and accurate pathological diagnoses to be made permitting oncologists to provide personalized oncotherapy to patients, which cannot be achieved by imaging alone. Single use of needles and onsite histopathology (ROSE) will further improve diagnostic yield.
PP-03-114
Comparative study on clinical changes in patients taking bowel cleansers
Young Cheon Ra
Division of Gastroenterology And Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: There were not many studies that compared blood test results with each bowel cleansers in real world. In addition, several studies have been published on safety and efficacy in elderly patients, but no conclusions have been reached. so it was necessary to check what kind of results actually come out in the real world. In this regard, we attempted to conduct a retrospective comparative study obtained from patients within each group when using oral sulfate tablets compared to previously existing bowel cleansing agents.
Materials and methods: Data collection was prospectively conducted on patients aged 18 to 80, who visited for colonoscopy, who have taken OST or Low PEG or Very low PEG over the past year (Jan 2022 - Jan 2023). The evaluation variables are electrolyte changes, BUN/Cr, BBPS score, adenoma detection rate, adverse effects, and survey after colonoscopy.
Results: There were no significant differences in baseline characteristics and no significant difference in adenoma detection rate. there were no life-threatening side effects. When patients took Oral Sulfate Tablet , they often took them all, and it was found that taking them was much easier.
Conclusion: In real world, compared to classical bowel cleansing agents such as Low PEG and Very low PEG, OST’s efficacy and safety is not inferior. Based on this study’s survey, if this is a group of patients who had difficulty with bowel preparation due to previous PEG, and additional colonoscopy is required, it would be a good idea to consider OST for the next examination.
PP-03-115
Feasibility of a novel unassisted single-channel transcolonic endoscopic appendectomy for the treatment of appendiceal lesions
Li Wang
Zhongshan Hospital, Fudan University, Shanghai, China;Endoscopy Center, Shanghai, Geriatric Medical Center, Shanghai, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Transcolonic endoscopic appendectomy (TEA) is rapidly evolving and has been reported as a minimally invasive alternative to appendectomy. We aimed to characterize the feasibility and safety of a novel unassisted single-channel TEA.
Materials and Methods: We retrospectively investigated 23 patients with appendicitis or appendiceal lesions who underwent TEA from February 2016 to December 2022. We collected clinicopathological characteristics, procedure-related parameters, and follow-up data and analyzed the impact of previous abdominal surgery and traction technique.
Results: The mean age was 56.0 years. Of the 23 patients with appendiceal lesions, fourteen patients underwent TEA, and nine underwent traction-assisted TEA (T-TEA). Eight patients had previous abdominal surgery. The En bloc resection rate was 95.7%. The mean procedure duration was 91.1± 45.5 minutes, and the mean wound closure time was 29.4 ± 18.6 minutes. The wounds after endoscopic appendectomy were closed with clips (21.7%) or a combination of clip closure and endoloop reinforcement (78.3%), and the median number of clips was 7 (range, 3-15). Three patients (13.0%) experienced major adverse events, including two delayed perforations (laparoscopic surgery) and one infection (salvage endoscopic suture). During a median follow-up of 23 months, no residual or recurrent lesions were observed, and no recurrence of abdominal pain occurred. There were no significant differences between TEA and T-TEA groups and between patients with and without abdominal surgery groups in each factor.
Conclusions: Unassisted single-channel TEA for patients with appendiceal lesions has favorable short- and long-term outcomes. TEA can safely and effectively treat appendiceal disease in appropriately selected cases.
PP-03-116
Management of Delayed Bleeding of Peroral Endoscopic Myotomy: A retrospective single-center analysis and Brief Meta-analysis
Li Wang1,2, Shao-bin Luo1, Zu-Qiang Liu1, Quan-Lin Li1 and Ping-Hong Zhou1
1Zhongshan Hospital, Fudan University, Shanghai, China;2Endoscopy Center, Shanghai Geriatric Medical Center
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Delayed bleeding is a rare but important major adverse event (mAE) after endoscopic submucosal tunneling procedures, which is scarcely reported. We aimed to characterize the clinical characteristics of delayed bleeding and provide better management of this mAE.
Materials and Methods: From August 2010 to October 2022, we reviewed 3,852 patients with achalasia receiving peroral endoscopic myotomy (POEM) and 1,937 patients with upper GI tumors receiving submucosal tunneling endoscopic resection (STER). Among these, records of 22 patients (15 POEM, 7 STER) with delayed bleeding were collected. Clinical characteristics, treatment, and outcomes of delayed bleeding were analyzed.
Results: The mean age was 43.6 years. Ten patients (45.5%) were intratunnel bleeding, seven (31.8%) were intratunnel bleeding accompanied by mucosal bleeding, and 5 (22.7%) were mucosal bleeding. The most common accompanied symptoms were hematemesis, fever, and melena. The most common accompanied mAEs were fistula, pulmonary inflammation, and pleural effusion with atelectasis. The mean duration from ESTP to endoscopic intervention was 5.3±4.9 days. Active bleeding was identified in 21 patients (95.5%). The bleeding was successfully controlled by electrocoagulation (19 cases), endoscopic clipping (6 cases), and Sengstaken-Blakemore tube insertion (3 cases) and no patient required surgical intervention. The mean hemostatic procedure duration was 61.8±45.8 minutes. The mean post-bleeding hospital stay was 10.0±6.2 days. A brief meta-analysis of previous studies showed the pooled estimate delayed bleeding rate after POEM, STER, and G-POEM was 0.4%.
Conclusions: Delayed bleeding is uncommon and could be effectively managed by timely emergency endoscopic procedures without requiring subsequent surgical interventions.
PP-03-117
GERD-A Novel predictor,In hoarseness(Of voice) patients with assessment of impedance pH and acoustic voice analysis
Jay Chudasama
Topiwala National Medical College, Mumbai, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
A study of reflux and voice disorders suggests that up to 55% of patients with hoarseness have laryngopharyngeal reflux. Exact incidence of gastroesophageal reflux disease(GERD) is not known in these sets of patients. Relation of acoustic parameters with GERD is also not studied well. Aim of the study was to investigate frequency of GERD in patients with hoarseness of voice and to study acoustic voice parameters & 24-hour impedance pH-metry finding in hoarseness with GERD patients.
Methods: 406 patients identified with hoarseness of voice of more than 6 weeks in ENT OPD, speech clinic and gastroenterology OPD combined.On basis of history, indirect laryngoscopy and speech parameters assessment ,374 patients were excluded for smoking, neurological, infectious causes of hoarseness.Remaining 32 patients underwent upper GI scopy, oesophageal manometry and 24-hour pH-metry.GERDwas diagnosed on basis of Lyon consesus.
Results: After exclusion,32 individuals with hoarseness of voice were evaluated further. Most common associated typical symptom were regurgitation (67%) and heart burn (50%). Mean DeMeester Score was 14.20 ±16.6. Mean AET was 3.96± 5.05 %. Mean No.Of Reflux Episodes 99± 84. Total 19 individuals diagnosed with GERD by using endoscopy(3) and impedance pH analysis(16) criteria.
12 acoustic parameters were studied in relation to GERD.
On univariate analysis, Jitter %, Absolute jitter, Pitch perturbation quotient, Shimmer were significantly associated with GERD (p<0.05). On multivariate analysis, Absolute jitter and Shimmer( P < 0.05) were independent predictors for GERD.
Conclusions: GERD was cause of idiopathic hoarseness of voice in 7.88 % patients.Speech parameters absolute Jitter and Simmer(db) are independent predictors of GERD.
PP-03-118
Hiccups-you can’t think this one !
Jay Chudasama
Topiwala National Medical College, Mumbai, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
50 years old male presented with c/o Hiccups (Daily, Nocturnal, Not responding to PPI, Baclofen), Dysphagia(Solid, Liquid), Weight loss (4-5 kgs), Heartburn since 2 years without any previous comorbidities. Upper GI scopy showed LA Grade C esophagitis without any food residue with Narrowing at GEJ(Ekcardt score–2) Impression was kept as ?Peptic esophageal stricture. Stricture was dilated with SG dilator(14 mm) and PPI was given for 1 month. 6 months later, No improvement in symptoms were noted with persistent hiccups and heartburn with progression of dysphagia from occasional to with every meal with Weight loss of additional 5 kgs. Repeat endoscopy showed normal esophagus with passage of scope across GEJ with moderate difficulty(Ekcardt score–5).High resolution manometry was performed which showed Mean IRP of 10 wet swallows -7.9 mm Hg,LES pressure – 14.1 mm Hg,40 % swallows showed premature contraction(DL <4.5 sec). Barium swallow showed luminal narrowing at GEJ with hold up of barium column in lower half of esophagus s/o outflow obstruction. Diagnosis was made as Distal esophageal spasm. Patient was managed with POEM with long myotomy (8 cm).2 weeks post dilatation, patient was symptom free, hiccups disappeared(Ekcardt score-0).HRM(Post 2 weeks) showed Mean IRP of 10 wet swallows -11.27mm Hg, LES pressure – 40.1 mm Hg, normal distal latency > 4.5 sec with Normal study. It was a First case of distal esophageal spasm presenting as hiccups. It requires high index of suspicion for diagnosis. HRM and TBE should be attempted in intractable hiccups
PP-03-119
Understanding Barrett's Esophagus in Systemic Sclerosis: Insight into Phatogenesis and Clinical Management
Masyfuk Zuhdi Jamhur1, Fhathia Avisha2, Crisdina Suseno3 and Rizki Trismimanda4
1General Practitioner, Medical Faculty Of Andalas University, Padang, Indonesia;2Departement of Internal Medicine, Ciptomangunkusumo Hospital, Jakarta, Indonesia;3General Practitioner, Mentawai Island District Hospital, Mentawai Regency Island, Indonesia;4General Practitioner, Kudungga District Hospital, East Kutai, Indonesia
Introduction: Gastrointestinal involvement in systemic sclerosis is heterogeneous, ranging from asymptomatic to significant dysmotility. Severity of GI involvement has shown to be a predictor of worse prognosis and mortality in patients with systemic sclerosis. An increased risk of Barrett esophagus in systemic sclerosis due to chronic reflux. We report a case of Barrett’s Esophagus in patients with systemic sclerosis.
Case Description: A 35-year-old female with a long history of diffuse cutaneous systemic sclerosis (mRSS 27) treated with mycophenolate sodium. The patient comes with chief complaints of heartburn, regurgitation, chest pain, and dysphagia for one year. The esophagogastroduodenoscopy examination showed esophagitis, pangastritis, and hiatal hernia. An abdominal ultrasound examination showed complex ascites. Biopsies confirmed Barrett’s esophagus. The patient was treated with lifestyle modification, PPI, Pro-motility agents, and mucoprotective. The Patient showed clinical improvement in GERD symptoms
Discussion: Barrett's esophagus is a transformative process wherein acid exposure causes the endogenous stratified squamous epithelium in the lower region of the esophagus to change into a metaplastic columnar epithelium. In systemic sclerosis, esophageal involvement can result from vasculopathy and tissue hypoxia, leading to dysphagia and GERD. Persistent acid reflux increases the risk of Barrett’s esophagus which can progress to adenocarcinoma. Patients with Barrett’s esophagus should be treated with PPI. The management is mainly aimed at alleviating the symptoms and preventing complications. Early detection is very important to prevent malignancy. Routine screening for Barrett’s esophagus is essential because of the high risk of neoplastic progression.
Esophageal Gastrointestinal Stromal Tumor: Incidental Finding During Urologic Workup of a Filipino Patient
Dovenne Jade Cania and Jenny Limquiaco
Cebu Doctors' University Hospital, Cebu City, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. It most commonly arises from the stomach and less commonly from the small intestine and colorectum. GIST which arises from the esophagus is a very rare occurrence and is only seen in <1% of all GISTs. Most cases are asymptomatic and is discovered incidentally for workup of another entity.
Clinical Presentation: Index case is a 65 year-old male businessman from Cebu who presented with an incidental finding of submucosal distal esophageal mass on CT-scan during work-up for a urologic pathology. Patient has no known comorbidities other than benign prostatic hypertrophy. He has no gastrointestinal complaints and has largely unremarkable physical examination. Contrast abdominal and chest CT-scan confirmed and delineated the distal esophageal mass. Patient underwent abdominal laparoscopic excision with Nissen fundoplication. Post-operative course was unremarkable as well and patient was discharge in stable condition. Final histopathology of the esophageal mass was consistent with gastrointestinal stromal tumor with a positive immunostaining for CD117. Patient was then referred to oncology service and is currently undergoing imatinib therapy.
Discussion: Esophageal GIST is a rare disease entity and is usually asymptomatic. As in all subepithelial tumors, prompt removal of tumor is part of definitive management. While large evidences are still lacking, it has been recommended that imatinib be given for all extra-gastric GISTs.
PP-03-121
Prognostic Factors Of Mortality In Biliary Tract Cancer : A Systematic Review
Fernando Dharmaraja1, Felix Wijovi1, Angeline Tancherla1, Ignatius Bima Prasetyo2 and Andree Kurniawan2
1Univesitas Pelita Harapan, Tangerang, Indonesia;2Internal Medicine Department Siloam Hospitals lippo Vilage, Tangerang, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: Biliary tract cancer is a rare gastrointestinal cancer. Despite known prognostic factors, the mortality rate remains high. This paper reviews and evaluates prognostic factors affecting mortality rates in biliary tract cancer patients.
Materials and Methods: Studies from PubMed, PMC, and ScienceDirect were collected using keywords “Biliary Tract cancer,” “Prognostic Factor,” and “Mortality rate.” Only English full-text studies published in the past five years were included. Data on prognostic factors and overall survival (OS) were analyzed. The risk of bias was assessed using the Quality in Prognosis Studies (QUIPS) tool.
Results: Twenty-five studies involving 8,414 patients were reviewed. Significant prognostic factors for OS were identified: demographic factors (old age, male sex), nutritional factors (low pre-operative cachexia index, low prognostic nutritional index, radiological sarcopenia), and laboratory factors (elevated serum lactate dehydrogenase, neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, platelet-to-lymphocyte ratio, CEA > 10 μg/L, CA19-9 > 39 IU/ml, reduced albumin levels, elevated CRP). Types of treatment (RFA, radiotherapy, lymphadenectomy, FFP infusion) and tumor characteristics (poor differentiation, gallbladder tumor, diameter > 5 cm, multiple tumors, ALB ≥ 35 g/L, lymph node metastasis, lymphovascular invasion, proximal ductal margin) were also identified. Tumor expression (β-catenin, DKK1+, Tropomyosin receptor kinase+, pyruvate kinase M2 upregulation, CD3+, CD8+, Foxp3+, HER2+, Leucine-Rich α-2-Glycoprotein-1 upregulation, low ribosomal receptor for activated C-kinase 1 expression), genetic factors (CDKN2B-AS1 overexpression, Transforming growth factor β CircLTBP2), and comorbidities (infectious complications) were significant as well.
Conclusion: This review highlights common and newly identified prognostic factors, expected to guide patient-centered clinical management to reduce biliary tract cancer mortality.
PP-03-122
Hematological Parameters Comparison In Colorectal Cancer, Non Colorectal Cancer Patients: Focus in NLR, PLR, LMR
Ety Febrianti
Sriwijaya University, Palembang, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract
Introduction: Colorectal cancer (CRC) is a gastrointestinal malignancy with high morbidity and mortality rates in Indonesia. Histopatology remains the gold standard for CRC detection and screening. Chronic inflammation and hosts immune responses are known to play important roles in tumorigenesis and cancer progression. This inflammation affects the results of hematological examination. Therefore, parameters such as Neutrophil-Lymphocyte Ratio (NLR), Platelet-Lymphocyte Ratio (PLR), and Lymphocyte-Monocyte Ratio (LMR) are expected to provide information on tumor cell development.
Objective: This study aims to evaluate the role of NLR, PLR, and LMR in distinguishing CRC and non-CRC at hospital Dr. Mohammad Hoesin Palembang.
Methods: The study was conducted using a cross-sectional design with a total of 141 suspected CRC patients, with 71 CRC patients and 70 non-CRC patients undergoing colonoscopy and histopathology examinations from April to August 2024.
Results: There was a comparison of the mean NLR ratio in the CRC group (4.6 ± 6.4) and non-CRC (3.9 ± 4.07). Comparison of the mean PLR ratio in the CRC group (19.3 ± 16.05) and non-CRC (20.8 ± 29.36). Comparison of the mean LMR ratio in the CRC group (3.18 ± 1.85) and non-CRC (3.3 ± 1.8).
Conclusion: An increased NLR ratio and a decreased PLR and LMR ratio can be potential parameters in differentiating CRC and non-CRC patients.
Keywords: NLR, PLR, LMR, CRC, Non-CRC
PP-03-123
Clinicopathological Profile of Gastric Cancer Patients at Cipto Mangunkusumo National Hospital: A Five-Year Review (2018-2023)
Yovita Gotama1, Steven Alvianto1 and Rabbinu Rangga Pribadi2
1School Of Medicine And Health Sciences, Atma Jaya Catholic University Of Indonesia, Jakarta, Indonesia;2Division of Gastroenterology, Pancreatobiliary, and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The study highlights gastric cancer's critical demographic and clinical characteristics at Cipto Mangunkusumo National General Hospital between January 2018 and December 2023.
Materials and Methods: A retrospective study was conducted using medical records of patients diagnosed with gastric cancer at Cipto Mangunkusumo National General Hospital between January 2018 and December 2023. Data collected included demographic information (gender, age, ethnicity, religion), clinical presentation (symptoms), type of gastric cancer, location, metastatic, and treatment modalities. Statistical analysis was performed to identify trends.
Results: A total of 300 patients were diagnosed with gastric cancer during the study period. The patients were divided into 60 patients with early onset (≤45 years) and 240 patients with conventional (>45 years). There was a significantly higher proportion of the conventional group comorbid hypertension compared with the early-onset group (0 and 13.75%; p=0.000) and also the symptoms of abdominal pain (35.4% and 13.3%, p=0.001). Otherwise, in the early-onset group, significantly higher abdominal distention compared to the conventional group (13.3% and 5%, p=0.037). The most common tumour location for both groups is non-cardia and the histological type from WHO classification is intestinal type. The majority of metastasis is liver (18.3%), lung (5.7%), and peritoneum (2.3%). The modality treatment for resectable cancer through surgery is also 46.3% of the total patients.
PP-03-124
Comprehensive meta-analysis of cd44 as diagnostic marker, prognostic and targeting therapy for colorectal cancer
Burhan Gunawan and Raymond Sebastian Purwanta
Sumber Waras Hospital, West Jakarta, Jakarta Barat, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: Activation number of signalling pathway by CD44 linked to tumour growth, migration, chemoresistance. But its role in CRC remains unclear. Aim of this study was to investigate CD44 expression, relationship to prognosis, and quantify clinical effect various drug targeting CD44 in CRC.
Method: The expression level of CD44 in colorectal cancer was analysed in TCGA, GEPIA2, HPA, and GEO database. The Kaplan-Meier Plotter database was used to analyse the relationship between CD44 expression and prognostic value (OS, PFS, PPS). The clinical study used CD44 targeting drug for colorectal cancer included in network meta-analysis using Revman Cochrane Library, following PRISMA guideline.
Result: The median expression value of CD44 in COAD tumour sample was 151.88 and in normal sample was 49.38, and CNV CD44 found in 78/453 (17.21%) subject. The CD44 significantly highly expressed in colon cancer sample compare to normal adjacent tissue sample (p=0.008). Immunohistochemically stained tissue sampel showed that CD44 significantly overexpressed in tumor tissue. The PFS of CRC patient with high CD44 expression were significantly poor (p=0.0098). Total 10 RCT Phase 1-3 Studies, use CD44 Targeting Drug or Antibody Monoclonal, with total 2627 subjects, included in meta-analysis. The PFS mean difference was 2.40 (95% C.I. 1.15-3.65) month, p=0.0002, I² = 100%.
Conclusion: CD44 is highly expressed in CRC which can be used as diagnostic and prognostic marker. This is also the first meta-analysis study about CD44 as targeting therapy in human CRC. The study shows the potential drug to regulating CD44 expression in the future for CRC.
PP-03-125
Berberine exhibits the capacity to impede the progression of gastric cancer by modulating oxidative stress
Xiaodong Han and Xin Chen
Tianjin Medical University General Hospital, Tianjin, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract
Objective: The goal of this study is to elucidate the mechanisms underlying the anti-gastric cancer effects of berberine using network pharmacology and subsequently validate these findings through in vitro experiments.
Materials and Methods: Target information for the effects of berberine and gastric cancer were collected from databases, and the intersection of these targets were used to construct a "drug-disease-target" network as well as a protein-protein interaction (PPI) network. The shared targets underwent GO functional and KEGG pathway enrichment analyses. In vitro experiments were conducted to investigate the impact of berberine on the activity, migration ability, and intracellular ROS levels of AGS and MKN45 gastric cancer cells. Western blot analysis was utilized to examine changes in the Nrf2/HO-1, HIF-1α, and EMT pathways.
Results: The network pharmacology analysis identified 224 common targets for berberine and gastric cancer. The GO analysis revealed that the top biological processes involving these targets included responses to oxidative stress, oxygen levels, oxidative stress in cells, reactive oxygen species, and hypoxia. The KEGG analysis indicated that pathways such as PI3K-AKT and HIF-1 may be key signaling pathways for the anti-gastric cancer effects of berberine. The in vitro experimental results demonstrated that berberine significantly suppressed the activity and migration ability of gastric cancer cells, elevated intracellular ROS levels, and inhibited the expression of proteins involved in the Nrf2/HO-1, HIF-1α, and EMT pathways in the cells.
Conclusion: Modulating oxidative stress may represent a crucial mechanism through which berberine inhibits the progression of gastric cancer.
PP-03-126
Incidence and Mortality of Cancer of Rectum and Anus in Chinese: a trend analysis
Junjie Huang, Shui Hang Chow, Chenwen Zhong, Sze Chai Chan and Martin Wong
The Chinese University Of Hong Kong, Sha Tin, Hong Kong
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background and Aims: This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of rectum and anus cancer.
Methods: Data on new cases and deaths related to rectum and anus cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).
Results: In 2020, a total of 1,898 new cases of rectum and anus cancer were reported in Hong Kong, with an ASR of 11.6. The incidence was significantly higher among males (ASR=15.1) compared to females (ASR=8.4), and in the older population aged over 50 years (ASR=49.1) compared to the younger population (ASR=2.2). There were 745 newly reported rectum and anus cancer-related deaths in Hong Kong, with an ASR of 4.3. The mortality was also notably higher in males (ASR=5.2) than in females (ASR=3.1), and in the older population (ASR=17.4) compared to the younger population (ASR=0.3). Trend analysis revealed a significant decreasing incidence of rectum and anus cancer in the older age group (AAPC: -1.6, 95% CI: -3.1, -0.01, P=0.05) and among females (AAPC: -1.8, 95% CI: -3.1, -0.4, P=0.02).
Conclusion: This population-based study demonstrated a decreasing trend in the incidence of rectum and anus cancer, particularly among the older population and females in Hong Kong. However, the overall disease burden remains significant, highlighting the need for further reducing the burden of rectum and anus cancer in the region.
PP-03-127
Incidence, Mortality and Trends of Oesophageal Cancer in Chinese: a population-based study
Junjie Huang, Ching Fung Yat, Chenwen Zhong, Sze Chai Chan and Martin Wong
The Chinese University of Hong Kong, Sha Tin, Hong Kong
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background and Aims: This study aimed to provide a comprehensive analysis of the oesophageal cancer burden, including the incidence and mortality trends by sex and age group.
Methodology: Data on new oesophageal cancer cases and deaths were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated using the Segi-Doll world population as the standard. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC) in the incidence and mortality of oesophageal cancer, stratified by sex and age group.
Results: In 2020, there were 399 newly reported oesophageal cancer cases (ASR=2.3) and 318 oesophageal cancer-related deaths (ASR=1.8) in Hong Kong. The incidence and mortality of oesophageal cancer were higher among males compared to females (incidence ASR: 4.6 vs. 0.7; mortality ASR: 3.2 vs. 0.5) and in the older population aged 50 years and above (incidence ASR: 10.6 vs. 0.2; mortality ASR: 8.14 vs. 0.18). Both the incidence and mortality trends of oesophageal cancer showed decreasing patterns over time, with a notable decline observed in the older population for both incidence (AAPC: -3.65, 95% CI: -5.01, -2.27, p<0.001) and mortality (AAPC: -3.93, 95% CI: -4.98, -2.87, p<0.001).
Conclusion: This study revealed an overall decreasing trend in the incidence and mortality of oesophageal cancer in Hong Kong, particularly among the older population. Despite this favorable trend, continued monitoring and the promotion of healthy lifestyles are essential to maintain the decreasing pattern of oesophageal cancer burden in the Hong Kong population.
PP-03-128
Proton pump inhibitors and gastric cancer: A large-scale propensity score-matched analysis using the UK Biobank
Cheal Wung Huh1, Da Hyun Jung2 and Hae-Ryong Yun1
1Yongin Severance Hospital, Yonsei University College Of Medicine, Seoul, South Korea;2Severance Hospital, Yonsei University College Of Medicine, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: We aimed to elucidate whether new proton pump inhibitor (PPI) users are at increased risk of gastric cancer compared with non-PPI users or new histamine-2 receptor antagonist (H2RA) users.
Design: A population-based cohort study was conducted using data from the UK Biobank from 1990–2016. We compared the incidence rates of gastric cancer after a minimum of 1-year drug exposure between PPI users and non-PPI or H2RA users using Cox proportional hazards models. Secondary analyses assessed duration and dose–response associations. Sensitivity analyses, incorporating various matching ratios, extended lag periods, and propensity score stratification, were performed.
Results: After large-scale propensity score matching, we included 42,732 new PPI, 42,732 non-PPI, and 4,762 new H2RA users. During a median follow-up of 6.7 years, PPI users had a 2.32-fold higher risk of gastric cancer than non-PPI users (HR 2.324, 95% CI 1.646–3.282), with numbers needed to harm of 488 and 442 at 2 and 4 years, respectively. Compared with H2RA users, PPI users also showed a significantly higher incidence of gastric cancer (HR 5.343, 95% CI 1.557–18.335). The risk of gastric cancer tended to increase with prolonged cumulative duration and higher cumulative doses of PPI use. Moreover, these results remained robust after adjustments for various lag periods, matching ratios, and propensity score stratification.
Conclusion: PPI use was associated with a higher incidence of gastric cancer, albeit with a low absolute risk. Therefore, long-term PPI use should be approached cautiously, even in regions with a low risk of gastric cancer.
PP-03-129
FOLFIRINOX Versus Gemcitabine/Nab-paclitaxel in Metastatic Pancreatic Cancer: A Real-World Data Analysis Including NGS Panel Insights
Jeehoon Kim, Galam Leem, Hee Seung Lee, Moon Jae Chung, Jeong Youp Park, Seungmin Bang, Seung Woo Park, Si Young Song and Jung Hyun Jo
Yonsei University College of Medicine, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: FOLFIRINOX and Gemcitabine/Nab-paclitaxel are widely used 1st-line regimens in metastatic pancreatic cancer. We would like to present real-world data of 12 years of experience with both regimens including genomics insights.
Aims & Methods: Patients with metastatic disease were retrospectively included from January 2010 to December 2022. Outcomes were overall survival (OS) and progression free survival (PFS). Median survival time was calculated using Kaplan-Meier estimates adjusted with 1:1 Propensity Score Matching (PSM). Tissue Next Generation Sequencing (NGS) panel data was analyzed.
Results: A total of 521 patients, 251 patients received FOLFIRINOX and 270 patients received Gemcitabine/Nab-paclitaxel as a first-line regimen. FOLFIRINOX group were younger (61.8 vs. 65.9, p<0.001). Before PSM, FOLFIRINOX group showed prolonged median OS (11.1 vs 7.8 months, p=0.002) and PFS (8.8 vs. 5.8 months, p=0.002) compared to Gemcitabine/Nab-paclitaxel group. After PSM, FOLFIRINOX group still showed prolonged median OS (11.1 vs 9.0 months, p=0.010) and PFS (9.2 vs 6.4 months, p=0.001) than Gemcitabine/Nab-paclitaxel group. NGS was available from 31 and 22 patients in the FOFLIRINOX and Gemcitabine/Abraxane groups, respectively. The most frequently mutated genes were KRAS (94.3%), TP53 (84.9%), CDKN2A (24.5%), SMAD4 (24.5%), ARID1A(13.2%) and RNF43(9.4%). On Cox Regression Analysis, RNF43 mutation was correlated with poor PFS with Gemcitabine/Nab-paclitaxel (HR, 4.849; 95% CI, 1.150-20.450, p=0.032).
Conclusion: FOLFIRINOX showed prolonged OS and PFS compared to Gemcitabine/Nab-paclitaxel. The NGS data suggest RNF43 could be a potential predictive marker for the response to Gemcitabine/Nab-Paclitaxel, however, a larger size confirmation study is needed to validate.
PP-03-130
Predictive factors for malignant lesions among colonoscopy cohorts in a tertiary hospital in indonesia
Alvin Johan1 and Saskia Aziza Nursyirwan2
1Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia;2Gastroenterology Division, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To determine factors that may predict malignant lesions among patients who underwent colonoscopy in Indonesia
Materials and methods: This retrospective cohort study used colonoscopy registry data at Dr. Cipto Mangunkusumo General Hospital during the 2018-2022 period. Binomial logistic regression was performed to ascertain the effects of age, sex, education, job status, smoking history, unhealthy dietary habits, and gastrointestinal symptoms on the likelihood that participants have a malignant lesion on colonoscopy.
Results: Lesions suggestive of malignancy were found in 301 of 1,694 colonoscopy reports (17.7%).
Linearity of the continuous variables concerning the logit of the dependent variable was assessed, and a Bonferroni correction was applied using all 11 terms in the model resulting in statistical significance being accepted when p < .0045. Age as the continuous independent variable was linearly related to the logit of the dependent variable. There were no residuals. The logistic regression model was statistically significant, χ²(10) = 129.171, p < .001. The model explained 12.1% (Nagelkerke R2) of the variance in colorectal malignancy and correctly classified 82.5% of cases.
The positive predictive value was 57.69% and the negative predictive value was 82.9%. Of the nine predictor variables, six were statistically significant: older age, joblessness, gastrointestinal bleeding, and weight loss were associated with higher odds of malignant lesions. Female sex and higher education were associated with lower odds of malignant lesions. (as shown in the table)
Conclusion: Age, sex, job status, education level, symptoms of gastrointestinal bleeding, and weight loss were predictive of malignant lesions on colonoscopy.
PP-03-131
Long-term outcomes of gastric mucosa-associated lymphoid tissue lymphoma A multi-center retrospective study in Honam, Korea
Jin Won Kim1, Seon-Young Park1, Ga-Ram You2, Wan-Sik Lee2, Byung-Chul Jin3, Seung-Young Seo3, Jung-In Lee4, Young-Dae Kim4, Chan-Guk Park4, Dong-Han Yeom5 and Suck-Chei Choi5
1Chonnnam National University Hospital, 42, Jebong-ro, Dong-gu, Gwangju, South Korea;2Chonnam National University Hwasun Hospital, 322, Seoyang-ro, Hwasun-eup, Hwasun-gun, South Korea;3Jeonbuk National University Hospital, Jeonju-si, South Korea;4Chosun University Hospital, Gwangju, South Korea;5Wonkwang University Hospital, Iksan-si, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: MALT lymphoma constitutes 50% of all lymphomas in the stomach and is linked to Helicobacter pylori (H. pylori). This Study explores treatment effectiveness, remission, and relapse factors in gastric MALT lymphoma patients under various treatments
Methods: A retrospective review was performed of 398 patients with gastric MALT lymphoma from 5 hospitals in Honam Province over a 15-year period with a minimum follow-up of 48 weeks.
Results: Mean age at diagnosis was 55.5 years with 176 males. H. pylori infection was in 293 (73.6%) patients, isolated lesion in 229 (57.5%) and stage IE in 363 (91.2%). There were no significant differences in age, sex, and stage at the diagnosis between H. pylori-positive group (HPP) and H. pylori-negative group (HPN). In the HPP group, 290 patients received eradication, 74 radiotherapy and 18 chemotherapy. In the HPN group, 77 received eradication, 56 radiotherapy and 19 chemotherapy. Overall complete remission rate was 97.0% [98.3% in HPP and 93.3% in HPN (P=0.018)]. HPP had higher complete remission rate compared to HPN (aOR 4.1). 32(8.3%) patients relapsed [23(7.9%) in HPP and 10(10.2%) in HPN]. Non-IE Modified Ann Arbor Stage (HR 2.4), presence of LAP (HR 1.9) and lesions in multiple locations (HR 2.1) were correlated with higher cumulative incidence of relapse (CIR). The presence of LAP was correlated with higher CIR in multivariate Cox regression analysis (aHR 2.9).
Conclusions: H. pylori infection status is associated with complete remission rates and presence of LAP is associated with the relapse.
PP-03-133
Burden of young-onset colorectal cancer: The India perspective from Global Burden of Disease study 2021
Laalithya Konduru1,4, Simranjeet Singh Dahia1,2 and Arsh Garg3
1Flinders University, Adelaide, Australia;2The University of Adelaide, Adelaide, Australia;3Government Medical College, Patiala, India;4Sri Jagannath Health Care and Research Center, Dhanbad, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Colorectal cancer (CRC) is the third highest cause of death worldwide. The Global Burden of Disease (GBD) data reveals a rising trend in young-onset CRC (YOCRC; CRC presenting in patients aged 15-49 years) globally. However, the burden of YOCRC in India remains unknown. We aimed to investigate the burden of YOCRC in India using data from GBD 2021.
Materials and Methods: The GBD India Compare tool (1990-2021) was queried for “colon and rectal cancer” as the “cause” and “incidence,” “deaths,” and “DALYs” as the “measure” for the 15-49 years age group. The national and state-wise burden of YOCRC in India was determined, and the Human Development Index (HDI) was used to evaluate associations between human development and YOCRC burden. The YOCRC burden attributable to leading dietary, behavioral, and metabolic risk factors was also estimated.
Results: National YOCRC incidence, mortality, and DALY rates increased significantly, particularly among males, between 1990 and 2021. In 2021, YOCRC incidence was highest in Uttarakhand, Uttar Pradesh, and Kerala, with Uttar Pradesh showing the highest YOCRC burden among females nationwide. HDI was not associated with YOCRC burden. This contrasts with global trends, which showed that YOCRC burden increases with increasing HDI. Diets low in milk, whole grains, and calcium remained the most important risk factors of YOCRC from 1990 to 2021.
Conclusions: India's YOCRC burden is increasing, regardless of HDI, driven by dietary, behavioral, and metabolic risk factors. The findings highlight the need for targeted interventions and greater awareness to address this public health challenge.
PP-03-134
Deciphering Changes in Immune Cells Associated with H. pylori-Induced Gastric Carcinogenesis via Single-Cell RNA Transcriptomics
Yonghwan Kwon
School Of Medicine/kyungpook National University, Daegu, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Backgrounds: Tumor microenvironment (TME) complex cellular components and heterogeneity have not been fully resolved in gastric carcinogenesis. Helicobacter pylori (H. pylori) is known to be the major cause of gastric carcinogenesis, the relation between H. pylori and TME were not fully elucidated.
Methods: we performed single-cell profiling of H, pylori related normal gastric mucosa, gastric dysplasia (GD), and early gastric cancer (EGC), especially intestinal type, identifying alterations in TME cell states and compositions.
Results: Transcriptome profiles of 70483 cells in the H. pylori infected 7 normal, 5 gastric adenoma, and 4 EGAC tissue samples were analyzed from 12 patients. After dimension reduction and clustering, 10 clusters of cells were identified. In the analysis of macrophages decoded cellular divergence in premalignant and malignant tumors, we identified 10 key genes were overexpressed in EGC compared with normal and GD and SPP1+/INHBA+ macrophages were significantly overexpressed in EGC is a central coordinator of H. pylori related gastric carcinogenesis (Fig 1). the proportion of exhausted CD8+ T cells and regulatory T cells increased. The ratio of cytotoxic CD8+ T cells decreased as GD progressed to EGC.
PP-03-135
Malignancy Predicting Model of Pancreatic Cystic Neoplasm in Contrast-Enhanced CT by Machine-Learning and Radiomics
Hong Sik Lee and Jae Min Lee and Jong Jin Hyun and Jeong Whan Choi and Kang Won Lee
Korea University Anam Hopsital, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Accurate identification of PCNs is crucial due to their diverse characteristics. To improve diagnostic precision, this study employs radiomics and machine learning to develop a predictive model for PCN malignancy risk, utilizing CT imaging data.
Materials and Methods: This retrospective study included 71 pts who underwent op for PCN and had CT scans within 90 days before op. PCNs were categorized as benign or malignant based on pathological results. We conducted texture analysis on segmented cystic fluid using an image processing program, calculating the standard deviation of gray-scale histograms(SDGSH) within the segmented area to detect increased heterogeneity. From the volume of interest in PCNs, 824 radiomics features were extracted. Radiomics and clinical features were selected using a random forest regressor, and the PCN classification model was trained and validated using a random forest classifier with 20-fold stratified cross-validation.
Results: The SDGSH representing texture heterogeneity, increased progressively from the non-dysplasia group to the invasive carcinoma group, with significant differences noted. The SDGSH demonstrated good predictive power for malignancy in pre-op CT images, with an AUC of 0.81 (95% CI 0.67-0.95). An optimal cut-off value of 17.597 yielded 87.5% sensitivity and 69.6% specificity. In internal validation, the radiomics-based machine learning model showed a meaningful AUC of 0.83 (95% CI 0.72-0.94), with a sensitivity of 81.25% and a specificity of 64.10%.
Conclusions: Radiomics technology, specifically SDGSH correlating with textural heterogeneity, and machine learning models analyzing cyst texture heterogeneity in CT images, were found to be good predictors of malignancy risk in PCN.
PP-03-136
Association between nlr, mlr, plr, la, and cea levels in colorectal cancer patients with metastasis
Muhammad Nur Ikhsan Liwang1,2, Rini Rahmawarni Bachtiar1,2, Muhammad Luthfi Parewangi1,2, Nu’man AS Daud1,2, Fardah Akil1,2, Susanto H. Kusuma1,2 and Amelia Rifai1,2
1Division of Gastroentyerology-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia;2Centre of Gastroenterology-Hepatology HAM Akil, DR.Wahidin Sudirohusodo General Hospital, Makassar, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract
Objectives: Colorectal cancer (CRC) is the third most common cancer worldwide. Early-stage diagnosis with enhanced screening methods is vital as metastases and recurrences increase mortality. The study aims to determine the predictive value of NLR, MLR, PLR, LA, and CEA levels as the metastases predictor of colorectal cancer (CRC).
Materials and methods: A case-control study was conducted using secondary data (patient medical records) of colorectal cancer patients, CEA and CBC data at Dr Wahidin Sudirohusodo Hospital Makassar between January 2023 and June 2024. The data were grouped and then statistically analyzed using SPSS version 29.0 for Windows.
Results: The number of patients consisted of 34 males (55.7%) and 27 females (44.3%), the most age >60 years (49.2%), the most tumour location in the rectum (54.1%), with mean ± SD NLR: 3.06 ± 0.56, MLR: 2.26 ± 0.58, PLR 258.92 ± 98.7, LA 4574 ± 1230, and CEA level 49.98 ± 69.18. NLR cut-off value 2.56, PLR 258, MLR 2.38, LA 4413, and CEA 10 ng/mL. NLR, PLR and CEA values higher than the cut-off had a higher incidence of metastasis (p<0.05), while lower LA had a higher metastasis rate (p<0.05).
Conclusion: There is an association between NLR, PLR, CEA, and LA in the presence of metastasis in colorectal cancer.
PP-03-137
Cold Snare Polypectomy for Pouch Polyps After Colectomy in Familial Adenomatous Polyposis: A Case Report
Tatsuya Matsumoto
Kitakyushu Municipal Medical Center, Fukuoka, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Familial Adenomatous Polyposis (FAP) is an autosomal dominant disorder caused by APC gene mutations, characterized by multiple colorectal adenomas. Main causes of death in FAP patients include colorectal cancer, desmoid tumors, and upper gastrointestinal cancers. Even after total colectomy, adenoma and cancer risks persist in residual rectal mucosa and ileal pouch. This report presents a case where Cold Snare Polypectomy (CSP) effectively treated multiple ileal pouch polyps in an FAP patient post-subtotal colectomy.
Case: A 62-year-old male with FAP underwent total colectomy, subtotal rectal resection, and ileal J-pouch anal anastomosis at 34. For 10 years post-surgery, endoscopy revealed only solitary rectal polyps. At 53, multiple polyps were detected in residual rectal mucosa. Large polyps were removed by Endoscopic Submucosal Dissection (ESD), but numerous polyps subsequently appeared in residual rectal mucosa and ileal pouch.
CSP was performed every 6-12 months for newly developed polyps. No rectal or small intestinal cancer has been detected to date. At 61, the patient underwent endoscopic papillectomy for duodenal ampullary adenoma, with no high-risk tumors or cancer developing in the duodenum.
Discussion: CSP was the primary method for controlling multiple ileal pouch polyps, proving safe and minimally invasive. This approach may potentially avoid surgical intervention. However, further research is needed to establish long-term effectiveness and prognosis of CSP in such cases. This case suggests CSP may effectively control residual gastrointestinal tumors in FAP patients post-total colectomy, offering a promising alternative to more invasive treatments.
PP-03-138
Bioelectrical Impedance Analysis Study of Colorectal Cancer Patients in Indonesian Tertiary Hospital
Dewi Mustikarani
Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Various studies have linked the severity of cancer and a decrease in body composition. The use of Bio Impedance Analysis (BIA) in determining body composition analysis has been considered the better and more feasible choice in hospital settings. This study aims to find the association between BMI, fat mass (FM), fat mass index (FMI), free fat mass (FFM), free fat mass index (FFMI), cachexia, and cancer staging in Indonesian tertiary hospital.
Materials and methods: This is a cross-sectional study. The analysis was performed using BIA from Seca. The baseline data was taken during the patient's last visit in 2021-2024. No significance was found among BIA results, cachexia, and cancer staging. The data were analyzed using SPSS version 25.
Result: There are 22 colorectal patients in this study. The mean age is 52 years, and 11 patients are male. With CI 95%, there are significant associations between age and cancer stage (p=0.006), BMI and fat mass (p<0.001), BMI and FFM (p=0.004), and BMI and FFMI (p<0.001). There is no significance found among BMI, cachexia, and cancer staging.
Conclusion: The progressivity of the cancer and its treatment can cause metabolic changes that affect body composition. BMI correlates with fat mass and free fat mass in colorectal cancer patients is multifactorial, involving pre-existing body composition, metabolic changes, the presence of nutritional interventions, and physical activity levels. Further study with a bigger population is encouraged.
Keywords: colorectal cancer, BIA, BMI, fat mass, fat mass index, free fat mass, free fat mass index, cachexia
PP-03-139
Fat Mass and Free Fat Mass Index as a Determinant of Colorectal Cancer Stage Progression
Dewi Mustikarani and Saskia Nursyirwan
Dr. Cipto Mangunkusumo/faculty Of Medicine Universitas Indonesia, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The intricate relationship between body fat metrics, such as fat mass (FM) and free fat mass index (FFMI), and the progression of colorectal cancer (CRC) has garnered increasing interest. This study explores the association between FM and FFMI with cancer staging in a colorectal cancer population compared to non-cancerous patients in an Indonesian tertiary hospital.
Matherials and Methods: This cross-sectional study utilized Bioelectrical Impedance Analysis (BIA) tools from Seca to assess body composition. Data were randomly collected from colorectal cancer outpatients between 2021 and 2024. A comparison group of non-CRC patients was also included. Statistical analysis was performed using SPSS version 25.
Results: A total of 51 participants were included, with 25 diagnosed with colorectal cancer. The study revealed significant associations between FM and FFMI with the cancer stage (p=0.022 and p=0.001, respectively). Additionally, significant correlations were found between FM, FFMI, and the presence of cancer (p=0.000).
Conclusion: Our findings demonstrate that FM and FFMI, as measured by Bioelectrical Impedance Analysis, are significantly associated with CRC staging. We also found a significant association between cancer and non-cancerous groups. This supports the hypothesis that cancer progression influences body composition. Further research with larger cohorts is recommended to validate these findings and explore the interplay between body composition, cancer stage, and other factors such as age and gender.
Keywords: colorectal cancer, staging, Bioelectrical Impedance Analysis, fat mass, free fat mass index
PP-03-140
UGT1A1 Genotype and Irinotecan Efficacy in Colorectal Cancer: a Systematic Review
Cecilia Oktaria Permatadewi2, Kevin Tandarto1, Hery Djagat Purnomo2, Budi Setiawan3, Hesti Triwahyu Hutami2, Didik Indiarso2, Agung Prasetyo2 and Hirlan Hirlan2
1Department of Internal Medicine, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia;2Division of Gastroentero- Hepatology, Internal Medicine Department, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia;3Division of Hematology and Medical Oncology, Internal Medicine Department, Faculty of Medicine Diponegoro University, Dr.Kariadi Hospital, Semarang, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Colorectal cancer (CRC) remains a significant health burden globally. Irinotecan, a key chemotherapeutic agent, is metabolized by UDP-glucuronosyltransferase 1A1 (UGT1A1). This systematic review aims to elucidate the impact of UGT1A1 genotype on irinotecan outcomes in CRC patients.
Methods: A comprehensive search of Google Scholar, PubMed, Embase, and Cochrane Library databases was conducted following PRISMA 2020 guidelines. Studies assessing the association between UGT1A1 genotype and irinotecan response in CRC patients were included. Data extraction and quality assessment were performed independently by two reviewers.
Results: A total of 572 articles were screened from the database. Published studies revealed a significant association between UGT1A1 *28 homozygosity and increased risk of severe irinotecan-induced toxicity. Additionally, UGT1A1 *28 homozygotes exhibited inferior progression-free survival and overall survival compared to wild-type or heterozygous individuals. Despite variations in study characteristics, the overall findings consistently supported the role of UGT1A1 *28 genotype in modulating irinotecan outcomes in CRC. Variations in UGT1A1 genotype, notably the *28 allele, have been implicated in irinotecan toxicity and efficacy discrepancies.
Conclusion: Homozygosity for the *28 allele confers a higher risk of severe adverse events and poorer clinical outcomes. Testing for the UGT1A1 genotype allows for personalized dosing of irinotecan to minimize adverse effects and optimize therapeutic efficacy.
PP-03-142
Efficacy and safety of fecal microbiota transplantation in amytrophic lateral sclerosis
Jingshuang Yan, Yunsheng Yang, Jing Cui, Huixin Chen, Lihua Peng, Xiaoyang Lan and Shengyuan Yu
The First Medical Center, Chinese Pla General Hospital, Beijing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Amyotrophic lateral sclerosis(ALS) is a fatal, progressive neurodegenerative disease with unclear pathogenesis. The feasibility of fecal microbiota transplantation(FMT) for ALS with respiratory failure had been reported in two patients. Large clinical validation are needed.
Materials and methods: A single-arm clinical trial was performed. ALS patients were enrolled to receive FMT by colonoscopy for three times with 6-week-interval. Evaluations were carried out one day before each FMT. The primary measure was the Revised ALS Functioning Rating Scale(ALSFRS-R), and response was defined as the score decreased by ≤ 0 points. Secondary measures were the ALS Assessment Questionnaire(ALSAQ-40), 36-Item Short Form Survey(SF-36), Self-rating Depression Scale(SDS) and Self-rating Anxiety Scale(SAS). Safety was assessed by the number and severity of adverse events.
Results: Fifty-nine patients were recruited. A total of 34 patients were analyzed at the end point with 22 males and 12 females. The onset age was 52.88±10.20 years, disease duration of 23.12±15.84 months, ALSFRS-R of 36.97±7.36 at baseline.
Responses occurred in 55.88% of the patients after the first FMT, and 61.76% after the second FMT. The slope of ALSFRS-R was -0.60±0.50 points/month at baseline, -0.53±0.35 after the first FMT, and -0.50±0.34 after the second FMT. Measured by ALSFRS-R subitems, FMT treatment ameliorated the symptoms of bulbar and respiratory function.
The scores of ALSAQ-40, SF36, SDS also presented an improvement tendency. No adverse events were reported during the trial.
Conclusion: FMT showed efficacy and safety for ALS with slower functional decline. Large double-blinded randomized controlled trials are needed to further verification.
PP-03-143
Dual therapy for type 2 diabetes H.pylori infection and the effect on the glycated hemoglobin
Xiaoyong Wang and Xin Cao
The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Patients with diabetes are at a higher risk of failure of H. pylori eradication. This study aimed to evaluate the efficacy and safety of vonoprazan-amoxicillin (VA) dual therapy for the treatment of H. pylori infection in patients with T2DM and to assess the influence of H. pylori eradication on glycated hemoglobin A1C (A1C) level.
Materials and Methods: This prospective, single-arm clinical trial enrolled 75 patients with T2DM diagnosed with H. pylori infection. Patients were treated with VA dual regimen comprising vonoprazan (20 mg twice daily) and amoxicillin (750 mg thrice daily) for 14 d (14-d VA dual therapy). 13C urea breath test was performed at least 4 weeks after completion of treatment. The A1C levels were measured 3 months post-treatment.
Results: The eradication rates in the intention-to-treat analysis and per-protocol analysis were 84.0% (63/75) and 87.14% (61/70), respectively. Adverse events occurred in 10 patients (13.3%), with none of the patients developing any severe adverse event. Good compliance was noted in 93.3% (70/75) of patients. Cigarette smoking (P=0.011) and high A1C levels (P=0.019) were associated with failure to eradicate H. pylori. Patients in the successful eradication group showed a significant decrease in the A1C level 3 months post-treatment compared with pre-eradication level (7.70±1.05 vs 7.23±1.00%, P=0.006). The A1C levels in the treatment failure group showed no significant difference before and after treatment (8.06±0.61 vs 7.23±1.16%, P=0.117).
Conclusion: VA dual therapy is a safe and effective regimen in patients with T2DM. Eradication of H. pylori improves glycemic control in these patients.
PP-03-144
1 / 2 Outcome predictors for vedolizumab therapy in patients with ulcerative colitis
Ayoung Cho
Sevrance Hospital, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Little is known about the prognostic factors related to clinical outcomes during vedolizumab therapy in ulcerative colitis patients. We aimed to investigate outcome predictors for vedolizumab during induction and maintenance therapy in UC
Method: 73 patients with moderate to severe UC who received vedolizumab treatment at Severance-Hospital between 2017 and 2021 were included. The medical records of patients were retrospectively reviewed. For analysis, logistic regression analysis was used to analyze primary non-response during induction therapy, while Cox regression was used to analyze risk factors for relapse during maintenance therapy. Variables with a p-value < 0.1 in the univariate analysis were included in the multivariate analysis.
Results: The average age was 43.6 ± 15.7 years, and 61.6% were male. After 14th week of drug administration, primary non-responders were identified in 15(20.5%), with high baseline CRP(> 6 mg/dl) identified as a risk factor(odds ratio 4.181, 95% confidence interval [CI] 1.028-17.013). Meanwhile, among the 41 who achieved clinical remission, clinical relapse occurred in 12%, 30%, 42% at 1year, 3years, 5years during maintenance. In multivariate analysis, vedolizumab dose intensification (hazard ratio 2.952, 95% CI 1.054-8.267) and absence of past anti-TNF use (hazard ratio 4.272, 95% CI 1.443-12.651) were identified as independent predictors of clinical relapse.
Conclusion: UC patients who receiving vedolizumab, high CRP(> 6 mg/dl) was identified as a risk factor for primary non-response. Meanwhile, vedolizumab dose intensification and no previous anti-TNF use were risk factors for clinical relapse during maintenance. These prognosticators will be useful in selecting appropriate patients for vedolizumab treatment.
PP-03-145
Outcome predictors for vedolizumab therapy in patients with ulcerative colitis: A single-center retrospective cohort study
Ayoung Cho
Sevrance Hospital, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Little is known about the prognostic factors related to clinical outcomes during vedolizumab therapy in ulcerative colitis patients. We aimed to investigate outcome predictors for vedolizumab during induction and maintenance therapy in UC
Method: 73 patients with moderate to severe UC who received vedolizumab treatment at Severance-Hospital between 2017 and 2021 were included. The medical records of patients were retrospectively reviewed. For analysis, logistic regression analysis was used to analyze primary non-response during induction therapy, while Cox regression was used to analyze risk factors for relapse during maintenance therapy. Variables with a p-value < 0.1 in the univariate analysis were included in the multivariate analysis.
Results: The average age was 43.6 ± 15.7 years, and 61.6% were male. After 14th week of drug administration, primary non-responders were identified in 15(20.5%), with high baseline CRP(> 6 mg/dl) identified as a risk factor(odds ratio 4.181, 95% confidence interval [CI] 1.028-17.013). Meanwhile, among the 41 who achieved clinical remission, clinical relapse occurred in 12%, 30%, 42% at 1year, 3years, 5years during maintenance. In multivariate analysis, vedolizumab dose intensification (hazard ratio 2.952, 95% CI 1.054-8.267) and absence of past anti-TNF use (hazard ratio 4.272, 95% CI 1.443-12.651) were identified as independent predictors of clinical relapse.
Conclusion: UC patients who receiving vedolizumab, high CRP(> 6 mg/dl) was identified as a risk factor for primary non-response. Meanwhile, vedolizumab dose intensification and no previous anti-TNF use were risk factors for clinical relapse during maintenance. These prognosticators will be useful in selecting appropriate patients for vedolizumab treatment.
PP-03-146
Efficacy and Safety of Mirikizumab in Ulcerative Colitis: A Systematic Review
Mohamed Karam Allah Elkholy1, Mazen Negmeldin Aly Yassin2, Dina Osama Yehia Ibrahim3, Duha Milad Abdullah Abuklish4, Amro Mamdouh Abdelrehim5 and Safia Elshennawy
1College of Pharmacy Zagazig University, Zagazig, Egypt;2College of Medicine, Cairo University, Cairo, Egypt;3College of medicine Fayoum University, Fayoum, Egypt;4Cardiology department, Tripoli hospital center, internal medicine;5College of Medicine, Misr University for Science and Technology, Egypt
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Ulcerative Colitis management is challenging. Despite the availability of several medication classes, about 15% of patients may still need colectomy. Mirikizumab, a novel monoclonal antibody directed against the p19 subunit of IL23 selectively, has demonstrated promising results in clinical trials. This review examines the efficiency and safety of mirikizumab in patients with moderate-to-severe active ulcerative colitis.
Materials and Methods: Our literature search covered PubMed, Scopus, Web of Science, and Cochrane. We included only the clinical trials. The primary outcomes were clinical response, clinical remission, symptomatic remission, and safety profile.
Results: Of the 394 studies identified, 4 were included. In the induction phases, evaluating efficacy at week 12, showed that 61% of patients achieved clinical response, 23% achieved remission, and 46% achieved symptomatic remission after intravenous mirikizumab at different doses every 4 weeks.
During the extended induction phases, evaluating efficacy at week 24, showed 52% clinical response, 11% clinical remission, and 38% symptomatic remission in patients who did not achieve a clinical response during the induction phases.
In the maintenance phases, evaluating efficacy at week 52, 73% of patients achieved clinical response, 45% achieved remission, and 66% achieved symptomatic remission with subcutaneous mirikizumab every 4 weeks.
The incidence of adverse events leading to discontinuation varied from 1.6% in the initial induction phases to 3.3% in the extended induction phases and 1.9% in the maintenance phases.
Conclusion: This is the first systematic review of mirikizumab for UC patients. The results showed promising efficacy and acceptable tolerability, but further investigations are needed.
PP-03-147
Unhealing wound with enterocutaneous fistula as a presentation of Crohn's disease
Rendy Sidik and Mario Stefanus and Muhammad Firhat Idrus
Division of Gastroenterology, Department of Internal Medicine University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Inflammatory Bowel Disease, in this case Crohn's disease(CD), has the potential to cause extraintestinal complications such as fistulas and the occurrence of colorectal cancer. Fistula complications increase morbidity and mortality, sepsis, and malnutrition. Fistulas formed in CD pose a higher and urgent management challenge, requiring a multidisciplinary approach.
Case Report: A 52-year-old man was referred from a previous hospital with complaints of pus discharge from a non-healing wound on the right side of the abdomen for the last 2 weeks. Infected wound on the right side of the abdomen that comes and goes, accompanied by diarrhea 4-5 times a day for the past year. Incision drainage abscess surgery was done a year ago. Abdominal CT scan showed a localized abscess in the right abdomen region with fistulation with the ascending colon and the skin of the right abdomen wall. Colonoscopy revealed an irregular mass with ulcers and fistulation in the cecum area up to stenosis. Histological tissue examination showed high-grade dysplasia with negative TB PCR results. The patient was continued on antibiotic therapy and underwent surgery.
Discussion: Chronic and recurrent abdominal wall wounds after surgery should be suspected of having inflammatory bowel disease, in this case, Crohn's disease. The prevalence of Crohn's disease with enterocutaneous fistula occurs in 10% of cases, although it commonly occurs in the perianal region. With optimal therapy using a combination of surgery and biologic agents, we hope to achieve a better total healing rate and reduce the risk of colorectal cancer.
PP-03-148
Clinical Outcomes and Prognostic Factors in Crohn’s Disease Patients with Internal Fistulas
Su Ji Woo, Soo Jung Park, Jae Hee Cheon, Tae Il Kim and Jae Jun Park
Severance Hospital, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Although internal fistulas in Crohn's disease patients frequently result in complications and require surgical intervention, their outcome predictors are rarely known. This study investigated the long-term clinical outcomes and prognostic factors for CD patients with internal fistulas.
Methods: Data were retrospectively collected from CD patients diagnosed with internal fistulas between March 2003 and August 2023. Clinical outcomes, including the occurrence of complications with or without surgical intervention and fistula closure, were assessed as primary endpoints. Statistical analysis methods, including Kaplan-Meier analysis with the log-rank test and Cox regression analysis, were used.
Results: A total of 72 CD patients were included in the study, with a mean follow-up period of 1280 days. Fistula locations included entero-enteric or entero-colic (n=70, 97.2%), entero-vesical (n=2, 2.8%), and entero-vaginal (n=2, 2.8%). Regarding complications with or without surgical intervention, occurrences were 34.6% at one year, 53.0% at three years, and 54.7% at five years. Multivariate Cox analysis showed that immunomodulator use reduced the occurrence of complications (hazard ratio 0.395, 95% confidence interval [CI] 0.207-0.754), while a history of previous bowel resection was associated with an increased risk of complications (hazard ratio 1.874, 95% CI 1.001-3.516). Meanwhile, among 56 Crohn's disease patients with internal fistulas treated with immunomodulators or anti-TNF agents, fistula closure was confirmed in 10 patients (17.9%) during the follow-up period.
Conclusion: The use of immunomodulators was associated with a reduction in complications. Our results suggest that close monitoring is necessary, and that the appropriate use of immunomodulators can be beneficial in these patients.
PP-03-149
Fecal siderophore genes are potential biomarker for ulcerative colitis
Jingshuang Yan, Rongrong Ren, Zhengpeng Li, Wanyue Dan, Xiaohan Zhang, Xiaoyan Chi, Lihua Peng and Yunsheng Yang
The First Medical Center, Chinese PLA General Hospital, Beijing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Gut microbiome-related biomarkers have considerable potential for disease assessment and therapeutic guidance in UC. This study aims to explore the potential value of fecal siderophore genes as a non-invasive biomarker to evaluate UC activity.
Methods: We included 166 patients with active UC (37 mild, 83 moderate, and 46 severe cases) and 165 healthy controls (HCs). Quantitative real-time PCR were used for the detection of eight siderophore genes, including enterobactin (entF, fepA), salmochelin (iroB, iroN), aerobactin (iucA, iutA), and yersiniabactin (irp1, fyuA).
Results: Patients with UC showed higher siderophore gene total copy number than HCs (1182.49 vs. 176.44 copies/ng). A threshold of 2877.68 copies/ng distinguished patients with active UC from HCs, with 90.3% specificity and 33.1% sensitivity.
The total copy number of siderophore genes was significantly higher in patients with severe active UC than in those with moderate (3111.03 vs 1183.54 copies/ng) and mild (3111.03 vs 672.60 copies/ng) active UC. Patients with severe endoscopic activity also exhibited higher total copy number than that in patients with mild-to-moderate endoscopic activity (1318.02 vs. 723.10 copies/ng). A threshold of 10298.63 copies/ng achieved 92.5% specificity and 43.5% sensitivity in identifying severe active UC, and 94% specificity and 22.5% sensitivity in identifying endoscopic severe active UC. In parallel test with C-reactive protein, the sensitivity and specificity increased to 89.1% and 75.8% for the clinical severe active stage.
Conclusion: Total siderophore gene copy number was positively correlated with clinical and endoscopic disease activity in UC, and serve as a potential non-invasive biomarker for UC activity.
PP-03-150
Fecal siderophore gene potentially predict the responsiveness of fecal microbiota transplantation for active ulcerative colitis
Jingshuang Yan, Guanzhou Zhou, Rongrong Ren, Xiaohan Zhang, Zikai Wang, Lihua Peng and Yunsheng Yang
The First Medical Center, Chinese Pla General Hospital, Beijing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Predictive markers for the outcomes of fecal microbiota transplantation (FMT) in ulcerative colitis (UC) are poorly defined. We aimed to explore the potential value of siderophore genes as non-invasive biomarker for predicting FMT responsiveness.
Materials and methods: We enrolled patients with active UC (Mayo score ≥ 3) who underwent two FMT procedures. Fecal samples were collected before and 8 weeks after each FMT session and determined the total copy number of eight siderophore genes, including enterobactin (entF, fepA), salmochelin (iroB, iroN), aerobactin (iucA, iutA), and yersiniabactin (irp1, fyuA). The relationship between total siderophore genes copy number and FMT efficacy was analyzed.
Results: Seventy-two patients with UC underwent FMT. The UC clinical response and remission rates were 62.5% and 19.4% after the first FMT, which increased to 71.7% and 43.4%, respectively, after the second FMT. Compared with baseline, the total siderophore genes copy number significantly decreased in the responder group, from 1557.32 copies/ng to 251.90 copies/ng after the second FMT. In the non-responder group, the total copy number of siderophore genes showed an increasing trend after the second FMT, from 65.46 copies/ng to 330.78 copies/ng. The total baseline copy number was significantly higher in responders than in non-responders (P < 0.01). A baseline total copy number cutoff value of 289.63 copies/ng showed 85.5% sensitivity and 88.2% specificity in predicting FMT responsiveness.
Conclusion: The total copy number of fecal siderophore genes in patients with UC correlates with FMT response, providing a promising biomarker for predicting FMT responsiveness.
PP-03-151
The swollen gut: a case report of gastrointestinal amyloidosis
Kunhan Chiam
Tan Tock Seng Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Amyloidosis is an infiltrative disorder due to extracellular deposits of amyloid fibrils. They distort the tissue architecture and function. Incidence is 1 case per 100000 person-years in Western countries and classified into: Systemic and localized amyloidosis. We report a patient with amyloidosis localized to the GI tract complicating with malabsorption, hypoalbuminemia, and anasarca.
Case Description: A 64 year old male presented with 9 months of lower limb swelling, bloating, and found to have iron deficiency anemia with hypoalbuminemia. Computed tomography imaging showed thickening throughout the small bowel loops from duodenum to terminal ileum, with pleural effusions. Endoscopy showed swelling and friable mucosa in the duodenum, and transverse colon. Biopsies were taken and histology of the duodenum and transverse colon showed deposition of extracellular, amorphous eosinophilic materials in the lamina propria, and Congo red stains of the eosinophilic material exhibit apple green birefringent staining consistent with amyloidosis. Bone marrow aspirate with trephine, and abdominal fat pad biopsy were done which did not show any amyloid deposits. This was conclusive of localized GI amyloidosis without systemic involvement.
Relevant investigations: Hb 5.9g/dL, Ferritin 4ug/L, albumin <15 g/L
PP-03-152
Achalasia or not Achalasia-Is it as simple as we think ?
Jay Chudasama
Topiwala National Medical College, Mumbai, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
70 years old male presented with Difficulty in swallowing(Solid+, Liquid +) with Retrosternal pain without regurgitation, weight loss and any significant past history since 6 month.(Ekcardt score - 4)Upper GI scopy showed Normal study.36 channel solid state HRM showed Median IRP 16.9 mm Hg with premature peristalsis with compartmental pressurization in >50 % swallows. Impression was given EGJ outflow obstruction with compartmentalized pressurization. HRCT thorax was normal. Trial of PPI given and repeat HRM was advised after 6 months. Took PPI for 2 months but
Symptoms were persistent. HRM(36 channel water perfusion)preformed, On wet swallows, showed mean IRP of 10 wet swallows -10 mm Hg, LES pressure – 14.1 mm Hg, 40 % swallows showed Ineffective peristalsis(DCI <450).On cookie swallow mean was IRP -9 mm Hg, 100% swallowed showed ineffective peristalsis(DCI < 450).Rapid drink challenge(supine position) showed median IRP-14.9 mm Hg, LES pressure – 42 mm Hg, presence of Pan esophageal pressurization. Timed barium esophagogram showed Smooth narrowing at distal esophagus with 11 cm height of barium column at 2 minutes s/o outflow obstruction/achalasia. Achalasia Cardia type II(In evolution) diagnosis was made and managed with 35 mm pneumatic balloon dilatation[Ekcardt score – 0 (2 weeks post dilatation ),symptom free].EGJ outflow obstruction can be found in normal individuals, persistent symptoms should be thoroughly investigated. Provocative tests can be helpful in a diagnosis of inconclusive manometric findings. Supportive tests like TBE/EndoFLIP should be performed in such cases. Pneumatic balloon dilatation should be used to manage dysphagia in doubtful cases of achalasia.
PP-03-153
Gastric amyloidosis- a case report
Md. Musab Khalil
Sheikh Russel National Gastroliver Institute And Hospital, Dhaka, Bangladesh
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Abstract:
Introduction: Amyloidosis are abnormal misfolded proteins that are resistant to degradation. Due to defective metabolism, they may be deposited in various organs causing organ dysfunction. Localized amyloidosis is a rare disorder.
Case Presentation: We are describing a case of isolated Gastric amyloidosis who presented with recurrent hematemesis and melena due to gastric ulcers. Gastroscopy revealed multiple gastric ulcers of variable sizes and shapes. Biopsy followed by histopathology with Congo red staining revealed Gastric amyloidosis.
Conclusion: Amyloidosis is a rare disorder. Localized amyloidosis is much rarer compared to systemic amyloidosis. Compared to systemic amyloidosis, localized amyloidosis has a better prognosis. High level of suspicion is needed to diagnose amyloidosis as a rare case of gastric ulcer.
PP-03-154
A case of phlebosclerotic colitis
Fujuan Luan
The First Affiliated Hospital Of Soochow University, Suzhou, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: A man was admitted due to recurring abdominal pain present for 4 months. After undergoing an abdominal CT scan and colonoscopy, he was diagnosed with phlebosclerotic colitis (PC). Subsequent subtotal colectomy and postoperative pathological analysis confirmed this diagnosis.
Case Description: A 71-year-old man was admitted due to recurrent abdominal pain in the lower right quadrant for a period of four months. Physical examination revealed tenderness in the lower right quadrant without rebound. Abdominal CT scan showed thickening of the wall in the ascending colon and hepatic flexure, with multiple "thread-like" calcifications observed in both the colon wall and adjacent mesenteric area. Colonoscopy revealed dark bluish mucosa in the ascending colon and edematous erosive mucosa in the transverse colon. Despite two weeks of conservative treatment, the patient's abdominal pain did not relieve, leading to consideration of exploratory laparotomy. During surgery, we discovered a dark blackened colon wall extending from cecum to descending colon, ultimately resulting in subtotal colectomy being performed. Pathological examination revealed extensive fibrosis and scattered calcification within both submucosal and serosal layers. The venous walls within these layers exhibited thickening along with diffuse fibrosis, hyaline degeneration, and calcification.
Discussion: PC also known as idiopathic mesenteric venous sclerosis or idiopathic mesenteric venous enteritis, is characterized by extensive calcification of the branch of the superior mesenteric vein and the wall of the colonic vein, along with thickening of the right half of the colonic wall. PC is a type of ischemic colitis that is non-thrombotic and non-embolic.
PP-03-155
Protective effect of 1,2-propanediol on radiation-induced intestinal injury in mice
Jiwei Zhao and Gang Sun
Chinese PLA General Hospital, Beijing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to investigate the protective effect of 1,2-propanediol on radiation-induced intestinal injury in mice.
Materials and Methods: 1、Mice were divided into an irradiation group and a drug administration group. The proliferation and regeneration of crypts were observed, and the intestinal damage was assessed by measuring the length of intestinal villi. The 30-day survival rate was also observed.
2、Immunohistochemistry of Olfm4 and Lysozyme, along with immunofluorescence using Lgr5-EGFP-IRES-CRE/ERT2 mice, was employed to detect the survival, proliferation, and regeneration of intestinal stem cells (ISCs).
Results: 1、The number of regenerated crypts in the irradiation group sharply decreased from 135.06±6.72 to 18.73±3.49. Compared with the irradiation-only group, the drug administration group showed an increase in the number of crypts and a significant increase in villus length, with a 30-day survival rate of 100%.
2、In the irradiation group, the number of Olfm4+ cells in the intestinal crypts decreased. Compared with the control group, the 1,2-PD administration group showed a significant reduction in Olfm4+ cells within 48 hours. After 3.5 days, proliferation and regeneration increased, inhibiting the loss of Paneth cells, and the proliferation of Lgr5-EGFP+ISCs increased.
Conclusion: 1,2-Propanediol has a protective effect against radiation-induced intestinal injury.
PP-03-156
1,2-propanediol ameliorated radiation-induced intestinal injury in mice via regulating P53
Jiwei Zhao1, Gang Sun1 and Zuyin Yu2
1Chinese PLA General Hospital, Beijing, China;2Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: This study aims to investigate the protective effect of 1,2-propanediol (1,2-PD) on radiation-induced intestinal injury in mice via the P53 pathway.
Materials and Methods: IEC-6 cells from both the irradiation group and the 1,2-PD-treated group were collected at 0 h, 3 h, 6 h, 12 h, and 24 h, and PI staining was performed to examine the cell cycle status. After 24 hours of incubation with different concentrations of 1,2-PD, proteins were extracted for immunoblotting experiments to screen for molecular targets related to cell cycle regulation.
Total protein and RNA were extracted from intestinal crypt cells of mice at 6 h and 24 h post-irradiation. Protein immunoblotting and RT-qPCR assays were conducted to detect molecules related to the P53-PUMA pathway. Additionally, survival observations and intestinal BrdU immunohistochemical detection were performed using P53-/- mice.
Results: After 12 hours of 1,2-PD administration, IEC-6 cells exhibited cell cycle arrest. With increasing concentrations of 1,2-PD, phosphorylation at the Rb S87/811 sites of the cell cycle regulatory protein Rb showed a gradient inhibition effect, leading to the dephosphorylation of Rb protein into an active state, which inhibits cell cycle progression.
Administration of 1,2-PD significantly inhibited the expression of genes and proteins related to the P53 pathway, as well as their downstream target proteins such as PUMA, Bax, and P21, at 6 hours and 24 hours post-irradiation.
Conclusion: 1,2-Propanediol exerts protective effects against radiation-induced intestinal injury by inhibiting apoptosis of crypt cells mediated by the P53-PUMA pathway
PP-03-157
Ascitic fluid and serum homocysteine as a potential biomarker in spontaneous bacterial peritonitis: A meta-analysis
Rukesh Yadav
Maharajgunj Medical Campus, Institute Of Medicine, Tribhuvan University, Kathmandu, Nepal
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: A polymorphonuclear leukocyte cell count of greater than 250/mm³ is the primary diagnostic criterion for spontaneous bacterial peritonitis (SBP). This approach is invasive and may not be diagnostic for all SBP variants. Accessible biomarkers are therefore required for the diagnosis of SBP. The purpose of the meta-analysis was to evaluate the potential diagnostic utility of homocysteine for SBP.
Materials and Methods: From the inception to January 2024, the Pubmed, Google Scholar, and Embase were searched for studies that assessed homocysteine concentrations of cirrhotic patients with and without SBP in the serum and the ascitic fluid. Results comprised the mean difference (MD) in the homocysteine concentrations in the serum and ascitic fluid of the two groups with a 95% Confidence Interval (CI). According to the heterogeneity, the homocysteine values were analyzed using the Revman 5.1 program using either a fixed or random effect.
Results: In this meta-analysis, four studies with 533 cirrhotic patients were included. 182 had SBP and 351 did not have SBP. Based on three studies, the analysis revealed a significantly higher ascitic fluid homocysteine in SBP patients as compared to non-SBP patients (MD: 3.25, CI: 2.26-4.23, I²=78%, P value <0.00001, figure 1) Furthermore, serum homocysteine in SBP patients was significantly higher than the non-SBP patients (MD: 6.22, CI: 4.47-7.96, I²=0%, P value <0.00001).
Conclusions: This meta-analysis shows that patients with SBP have significantly higher serum and ascitic homocysteine levels than individuals without SBP. For any SBP variants, serum homocysteine may offer a trustworthy and noninvasive diagnostic value.
PP-03-158
MAFLD in Older Adults is Associated with Atrial Fibrillation and Physical Disability But Not Mortality
Daniel Clayton-Chubb1,2, Stuart Roberts1,2, Ammar Majeed1,2, Robyn Woods2, Andrew Tonkin2, Mark Nelson3, Andrew Chan4, Joanne Ryan2, Ms Cammie Tran2, Alexander Hodge2, John Lubel1,2, Hans Schneider1,2, Amy Brodtmann2, John McNeil2 and William Kemp1,2
1Alfred Health, Melbourne, Australia;2Monash University, Melbourne, Australia;3University of Tasmania, Australia;4Massachusetts General Hospital, Boston, USA
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The impact of Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) on older adults is understudied particularly in relation to cardiovascular disease-related outcomes. Therefore, we aimed to evaluate the impact of MAFLD on key health outcomes for older adults using data from the ASPirin in Reducing Events in the Elderly (ASPREE) trial and ASPREE-XT cohort.
Materials and Methods: ASPREE included 16,703 community-dwelling Australian participants aged ≥70 years without cardiovascular disease, dementia, or independence-limiting physical disability; 9,846 had complete data for this analysis. Anthropometric, biochemical, and questionnaire data were collected at baseline. Hepatic steatosis (HS) was defined as a Fatty Liver Index (FLI) score ≥ 60. MAFLD was defined as HS along with usual cardiometabolic criteria. FLI < 30 was used as a no-HS comparator.
Results: Of the 9,846, 38.0% (3,742) had MAFLD (mean age 75.0 ± 4.2 years, 53.2% female). MAFLD is strongly associated with persistent physical disability (aHR 1.46 [95% CI 1.19 – 1.80]) and atrial fibrillation (aHR 1.44 [95% CI 1.11 – 1.86]). While it’s associated with major adverse cardiovascular events (MACE) on unadjusted analyses (HR 1.42 [95% CI 1.17 – 1.71]), this association is lost when fully adjusted (aHR 1.18 [95% CI 0.95 – 1.47]). MAFLD is not associated with mortality (aHR 1.03 [95% CI 0.88 – 1.20]).
Conclusion: MAFLD is common in older adults, is related to atrial fibrillation, MACE, and physical disability, but does not confer an excess risk of all-cause mortality. These data have important public health implications.
PP-03-159
Disease Prevalence and Current Therapies for Metabolic Dysfunction-Associated Steatotic Liver Disease in India
Geeta S Desai1, Sandesha Ghorpade2 and Santosh Hajare3
1Department of Gastroenterology JNMC, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India;2Department of Gastroenterology, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India;3H.O.D., Department of Gastroenterology, KLES Dr Prabhakar Kore Hospital and MRC, Belagavi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The increasing global prevalence of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD), requires effective treatments. Saroglitazar, Vitamin E, Omega-3 fatty acids, and lifestyle modifications have shown varying effectiveness. This study aims to determine MASLD prevalence and evaluate treatment efficacy in North Karnataka.
Methods: From 2021, patients aged 18-55 without liver disease history were screened on various parameters excluding alcoholic and viral causes. Fatty liver on imaging indicated MASLD/MASH. We assessed treatments (Diet and Exercise, Saroglitazar, Vitamin E, Omega-3 fatty acids) in 100 MASLD/MASH patients in 1:1:1:1 ratio. Liver stiffness, transaminases, and triglycerides were monitored at baseline, 24 weeks, and 52 weeks.
Results: Total 3,348 patients screened, 1410 were diagnosed with MASLD/MASH. Among 1,026 analyzed patients, 12.34% had abnormal liver function tests, 45.53% had abnormal CAP, 27.23% had abnormal LSM, and 14.89% had abnormal LFTs. Among MASLD/MASH patients, 17.58% had diabetes and 2.55% had lean MASH. Among Treatment group age averages (in years): Diet and Exercise 43.04 ± 7.31, Saroglitazar 47.73 ± 6.52, Vitamin E 40.44 ± 8.03, Omega-3 FAs 41.72 ± 10.75. Over 52 weeks, liver stiffness changes were: Saroglitazar -5.18 KPa (-42.91%, P=0.001), Vitamin E -2.12 KPa (-19.40%, P=0.001), Diet and Exercise -1.33 KPa (-11.88%, P=0.001), Omega-3 FAs -0.45 KPa (-4.24%, P=0.342). Significant improvements in transaminases and triglycerides were noted for Saroglitazar and Vitamin E. No adverse events were reported.
Conclusion: The 42.11% prevalence of MASLD and MASH in North Karnataka underscores the urgent need for lifestyle awareness. Saroglitazar effectively improves liver health markers in MASLD, warranting further investigation.
PP-03-160
New Onset Metabolic Syndrome Associated with MAFLD in A Young Adult: A Case Report
Yovita Gotama, Anak Agung Istri Kumala Dewi, I Made Suma Wirawan and Made Wirama Diyana
Department of Internal Medicine, Wangaya Regional Hospital, Denpasar, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Metabolic-associated fatty liver disease (MAFLD) is increasingly recognized as a significant health issue linked to metabolic dysfunction – with an estimated global prevalence of 37%. Metabolic syndrome is a cluster of conditions, including obesity, insulin resistance, hypertension, and dyslipidemia. This case report explores a new onset of MAFLD in young adults diagnosed with metabolic syndrome, emphasizing the need for early recognition and intervention.
Case Description: A 33-year-old female with intermittent right upper abdominal pain. She has a medical history of well-controlled hypertension, but no history of diabetes or family history of diabetes. No history of alcoholism. The patient had a BMI of 26.6 kg/m2, categorized as grade I obesity, and a waist circumference of 100 cm. Laboratory tests revealed a fasting blood glucose of 240 mg/dL, HbA1C of 9.8%, HDL of 28 mg/dL, and triglycerides of 339 mg/dL. Liver function test results AST 48 (0-37) U/L and ALT 55 (0-42) U/L. USG examination showed a grade III fatty liver, cholelithiasis, and cholecystitis. The medication includes diet and healthy lifestyle, long-acting insulin combination with antidiabetic drugs, antihypertension, antibiotics, fibrate, and ursodeoxycholic acid.
Discussion: The patient presented with metabolic syndrome and liver function tests and USG results confirmed MAFLD. This case highlights MAFLD manifesting in younger populations, generally considered at lower risk. The mechanisms linked to insulin resistance, chronic inflammation, and lipid metabolism dysfunction. It is essential to assessed metabolic status and implementation of lifestyle interventions and medical management. Early intervention is needed to manage and mitigate the progression of MAFLD.
PP-03-161
Plasma Proteomic Signatures revealed altered Lipid/cholesterol metabolism, Coagulation and Complement pathways associated with Nonalcoholic steatohepatitis
Abhishak Gupta1,2,3, Puja Sakhuja2 and Shiv K Sarin3
1Artemis Hospitals, Gurugram, India;2G B Pant Hospital, DELHI, India;3Institute of Liver and Biliary Sciences, Delhi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Fat accumulates in the liver and serves as a defining feature of non-alcoholic fatty liver disease (NAFLD). Plasma is a reflection of the body's metabolic changes and a useful tool for identifying biomarkers in the disease state. Objectives: We aimed to standardize low abundance plasma proteins in order to find possible biomarkers for NAFLD/NASH.
Methods: Plasma proteomic profiles of a total of 74 plasma (25controls+54 NAFLD/NASH) were analyzed after depleted major proteins (globulins and albumin) using two-dimensional gel electrophoresis (2D-Gel) and coupled with MALDI-TOF. The differentially expressed proteins between the comparable groups were quantified and validated by ELISA.
Results: After depleting major plasma proteins, we observed ≈300 proteins on 2D-Gel electrophoresis. Sixty differentially expressed (2-4 folds) protein spots were identified by the Mascot search database. Our integrated analysis show seven (n=7) significantly different plasma proteins (p<0.05), such as Transferrin(TF), Transthyretin(TTR), Complement C3, Fatty acid binding protein(FABP), Apolipoprotein-A1(APO-A1), Haptoglobin(Hp) and Fibrinogen(FBG) were involved in lipid/cholesterol metabolism, coagulation and complement pathways in NASH patients. Two-proteins, TF and TTR, were down-regulated and others were up-regulated in NASH. Further, plasma A-FABP level was significantly higher in NAFLD patients as compared with controls (47.3±14.8 vs. 13.7±9.8ng/ml; p<0.001). We also observed significant correlation between circulating A-FABP levels and BMI in the subgroup of NAFLD patients who had BMI≥25 kg/m2(n=36,74%) as compared with those BMI≤25kg/m2 (n=18,26%)(42.4±19.2vs. 33.7±15.2ng/ml;P<0.01), respectively.
Conclusions: Plasma proteomic signatures reflect altered coagulation/complement pathway, cholesterol/lipid metabolism in NASH patients. A-FABP may also be a useful biomarker for prognosis and assessing disease severity.
PP-03-162
Correlation of Non-Invasive Fibrosis Scores with Vibration-Controlled Transient Elastography in MASLD Patients
KK Rashid, Jesse Jacob Skariah, S Sreekumar, A Shanid, Srijaya S Sreesh and Krishnadas Devadas
Government Medical College, Thiruvananthapuram, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Fibrosis is a key indicator of disease severity in Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD). Non-invasive tests (NITs) offer safer, more convenient alternatives to liver biopsy for assessing fibrosis. This study compares the NAFLD Fibrosis Score (NFS), Fibrosis-4 (FIB-4) index, and Aspartate Aminotransferase to Platelet Ratio Index (APRI) against Vibration-Controlled Transient Elastography (VCTE) in MASLD patients.
Materials and Methods: This retrospective observational study included 1169 MASLD patients who underwent VCTE. Fibrosis was staged by the APASL (Asian Pacific Association for the Study of the Liver) grading system based on VCTE. APRI, NFS, and FIB-4 index were calculated. The diagnostic performance of APRI, NFS, and FIB-4 in identifying significant fibrosis and above was assessed by calculating the area under the receiver operating characteristic curve (AUROC).
Results: Among the patients, 643 (55%) were males. Fibrosis stages were F0-F1 (65%), F2 (11%), F3 (16%), and F4 (8.2%). Correlation analysis between VCTE and other parameters, including APRI, FIB-4, and NFS, showed statistically significant correlations (Pearson correlation coefficients, r= 0.349, 0.475, and 0.542, respectively). ROC curve analysis demonstrated the predictive capability of various parameters for fibrosis (VCTE > 8.2), with AUROC values of 0.713 for APRI, 0.799 for FIB-4, and 0.852 for NFS.
Conclusion: Non-invasive scoring systems like APRI, FIB-4, and NFS are effective bedside tools for diagnosing liver fibrosis in MASLD patients. These tests offer a streamlined approach to diagnosis, enhancing patient care by reducing the need for invasive procedures in MASLD
PP-03-163
Investigating the Relationship Between Hepatic Steatosis and Fibrosis in Chronic Hepatitis B Patients
K Cokorde Istri Yuliandari Krisnawardani1, Ketut Mariadi2, Gde Somayana2 and Komang Agus Wira Nugraha2
1Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/Udayana University Hospital, Denpasar, Indonesia;2Gastroenterohepatology Division, Internal Medicine Departement, Faculty of Medicine, Udayana University/RSUP Prof. Dr. I.G.N.G. Ngoerah, Denpasar, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: With the rising prevalence of obesity, hepatic steatosis often coexists with chronic hepatitis B (CHB) infection and may influence liver-related outcomes. This study aims to investigate the effect of hepatic steatosis on fibrosis progression in CHB patients.
Materials and Methods: This cross-sectional, single-center study included 303 CHB patients. Exclusions were chronic hepatitis C, alcoholic liver disease, hepatocellular carcinoma, and autoimmune liver disease. Anthropometric measurements (weight and height) were taken. Liver stiffness and controlled attenuation parameter (CAP) for hepatic steatosis were measured using transient elastography. Liver fibrosis was defined as TE stage F2-4, and hepatic steatosis and severe steatosis were defined as CAP ≥248 dB/m and ≥280 dB/m, respectively. Appropriate statistical analyses were conducted.
Results: The median age of 303 CHB patients (63.7% male) was 49 years old. Hepatic steatosis prevalence was 29.7%. Patients with steatosis had a higher BMI than those without (p<0.001). There was no significant difference in liver fibrosis between patients with and without steatosis (9.9 kPa vs. 10.41 kPa, p=0.371), even with increasing steatosis severity (p=0.452). Some patients in this study had already on nucleoside analogs therapy for a median duration of 6 months, yet no difference in fibrosis was found between the treated and untreated groups (9.2 kPa vs 11.9 kPa, p=0.941). Multivariate logistic regression showed increasing age and male sex were independently associated with liver fibrosis (AOR: 1.04, 95% CI: 1.01-1.06, p<0.001 and AOR: 3.41, 95% CI: 1.97-5.93, p<0.001 respectively).
Conclusion: The presence of hepatic steatosis was not associated with fibrosis in CHB patiens.
PP-03-164
Probiotics Improve Liver Function in Non-Alcoholic Fatty Liver Diseases: A Systematic Review and Meta-Analysis
Meti Metiani1, Idn Wibawa2 and Hasani Farhan3
1Department of Internal Medicine, Hermina Arcamanik Hospital, Bandung, Indonesia;2Department of Internal Medicine, Balimed Hospital, Denpasar, Indonesia;3Bandung Islamic University, Bandung, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: This review aimed to systematically analyze the effect of probiotics on improving liver function in NAFLD treatment.
Methods: The authors searched for best quality studies (RCTs) up to 1 January, 2012 in 3 databases (Pubmed, Cochrane Library, and Google Scholar) on administering probiotics to NAFLD patients. The risk of bias was assessed and the results were combined using specialized software. Mean differences between groups were analyzed with 95% confidence intervals. Heterogeneity between studies was examined to determine appropriate statistical models. Results are presented visually and interpreted with a significance level of p<0.05
Results: We analyzed 14 studies with 841 participants. The mean differences (MD) for the benefits of probiotics to reduce ALT were -3.05 (confidence interval [CI] -5.00, -1.10), to reduce AST were -0.56 (confidence interval [CI] -2.21, 1.09), to reduce GGT were -0.64 (confidence interval [CI] -163, 036), and to reduce ALP were 0.34 (confidence interval [CI] -4,99, 5.67). In addition, the benefits of probiotics on inflammatory markers were tested with TNF alpha were 0.46 (confidence interval [CI] -0.88, 1.81) and IL-6 were 0.42 (confidence interval [CI] -0.13, 0.96).
Conclusion: Probiotics improved liver enzyme values and inflammatory markers in NAFLD, but only the reduction in ALT levels was statistically significant.
PP-03-165
Probiotics as a Potential Treatment for Non-Alcoholic Fatty Liver Diseases: A Systematic Review and Meta-Analysis
Meti Metiani1, I Dewa Nyoman Wibawa2 and Hasani Farhan3
1Department of Internal Medicine, Hermina Arcamanik Hospital, Bandung, Indonesia;2Department of Internal Medicine, Balimed Hospital, Denpasar, Indonesia;3Bandung Islamic University, Bandung, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: To evaluate the effect of probiotics as a potential treatment for non-alcoholic fatty liver disease (NAFLD).
Materials and Methods: A systematic search was conducted in PubMed, Cochrane Library, and MEDLINE Complete for randomized control trials (RCTs) of probiotics in patients with NAFLD from 2011-2024. The results were meta-analyzed using RevMan 5.4 software and the Cochrane Risk-of-Bias 2 tool.
Results: The study included 18 RCTs with 865 patients diagnosed with NAFLD. The results showed that the administration of probiotics reduced liver enzyme levels; although only ALT (MD = −1.65, 95% CI [-2.39, -0.91], P < 0.0001) and GGT (MD = −5.81, 95% CI [-7.68, -3.93], P < 0.00001) were significantly decrease. Probiotics were found to decrease liver steatosis; however, the reduction was only significant for grade 1 (OR = 0.49, 95% CI [0.27, 0.89], P = 0.02) and grade 2 (OR = 0.33, 95% CI [0.18, 0.61], P = 0.0004). Improvement in liver fibrosis was assessed by transient elastography (Fibroscan) (MD = -0.65, 95% CI [-1.10, -0.20], P = 0.005), and probiotics were also shown to reduce levels of inflammatory markers; however, only TNF-α (MD = -1.46, 95% CI [-1.86, -1.07], P < 0.00001) and IL-6 (MD = −0.94, 95% CI [-1.30, -0.58], P < 0.00001) showed a significant decrease.
Conclusion: Probiotics have been shown to have a positive effect on NAFLD by normalizing liver enzyme, improving fibrosis, and reducing steatosis and inflammatory markers. Further study is needed to confirm the effect of probiotics and evaluate long-term efficacy and safety.
PP-03-166
Lifestyles associated with MASLD
Hirofumi Mikami and Akio Moriya
Mitoyo General Hostipal, Kanonji, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: To clarify what specific lifestyle habits affect MASLD.
Subjects and Methods: The subjects were 5866 participants who underwent health checkups including abdominal ultrasound examination between April 2014 and June 2022. We performed logistic regression analysis of the association with MASLD for exercise habits (>30 min), weight gain (more than 10 kg since age 20), high physical activity, walking speed, eating speed, dinner time, snack, skipping breakfast, alcohol consumption, and smoking.
Results: The prevalence of MASLD in the subjects was 16.4%; the median BMI was 23.0, the median age was 54 years, and 55% were male. Overweight or obesity was present in 53%, glucose intolerance in 62%, hypertension in 41%, and dyslipidemia in 34%.
Results of logistic regression analysis adjusted for age, gender, and cardio metabolic risk factors showed that weight gain (odds ratio, 2.109; 95% confidence interval, 1.781-2.502) and high physical activity (0.830, 0.701-0.981) were the associated factors. When stratified by BMI, weight gain was an associated factor in normal weight, overweight, and obesity, excluding underweight. For normal weight only, high physical activity (odds ratio 0.668 for 95%, 0.450-0.978) and skipping breakfast (odds ratio 0.422 for 95%, 0.183-0.850) were also associated factors.
Conclusion: Weight gain (more than 10 kg since age 20) was a particularly important factor associated with MASLD. High physical activity and skipping breakfast were also associated with MASLD only in the normal weight group.
PP-03-167
Beyond biopsy:Comparing Vibration-controlled transient elastography and 2D-shear wave elastography in Metabolic dysfunction-associated steatotic liver disease
S Sreekumar, Arjun Haridas, Krishnadas Devadas and Srijaya Sreesh
Government Medical College, Thiruvananthapuram, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Vibration-controlled transient elastography(VCTE) and two-dimensional shear wave elastography(2D-SWE) are non-invasive techniques for liver stiffness measurement(LSM). We aimed to analyze the reproducibility of LSM on repeated VCTE and to compare LSM using 2D-SWE and VCTE in Metabolic dysfunction-associated steatotic liver disease(MASLD).
Materials and Methods: 275 patients from MASLD clinic at Government Medical College, Thiruvananthapuram were included. LSM using VCTE with FibroTouch-FT100 were performed twice, 2 hours apart, in fasting patients. LSM using 2D-SWE with SuperSonic Imagine’s-Aixplorer was also performed on the same day.
Results: The correlation coefficient between LSM on repeated VCTE was 0.96. The median LSM was 7.8(6.6-10.1)kPa and 8.3(6.8-10.4)kPa using VCTE and 2D-SWE respectively. The median difference in LSM between VCTE and 2D-SWE was 1.0kPa(0.5–1.5). In fibrosis ≤F2 and ≥F3, the difference was ±1.1kPa and ±0.9kPa respectively. Using Bland-Altman plot, significant disagreement between LSM values was noted in 21(7.63%). In multivariate analysis, central obesity and triceps skinfold thickness, were found to be independently associated with this. LSM values showed strong correlation(r=0.665). In fibrosis ≤F2 and ≥F3, correlation was 0.45 and 0.67 respectively. The area under the ROC curve for 2D-SWE in predicting ≥F3-fibrosis was 0.866. Based on Youden index, 2D-SWE cutoff of 9.9kPa had 79.5% sensitivity and 89.3% specificity in diagnosing ≥F3-fibrosis.
Conclusion: Repeated LSM using VCTE has high degree of reproducibility. The difference in LSM between both techniques was more at lower values of LSM and showed stronger correlation in higher degrees of fibrosis. Given the strong diagnostic accuracy of 2D-SWE, both techniques could be used interchangeably.
PP-03-168
Fucoidan alleviated NASH via inhibiting oxidative stress by promoting GSTA2
1Zhongshan Hospital, Fudan University, Shanghai, China;2Department of Gastroenterology and Hepatology, Zhongshan Hospital (Xiamen), Fudan University, Xiamen, China;3Shanghai Geriatric Medical Center, Shanghai, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: This study aims to explore the effect of fucoidan on non-alcoholic steatohepatitis (NASH) and the underlying molecular mechanisms by constructing a 3D NASH model based on Nucleic-Acid-nanostructures-decorated-living-Cells (NACs).
Materials and Methods: This study constructed NAC NASH models from human cell lines and murine primary cells using NACs. Effects of fucoidan on lipid accumulation, fibrosis, and inflammation in NAC NASH models were investigated through pathological, biochemical detection and ELISA assay. RNA-seq technology was used to explore the underlying molecular mechanisms.
Results: The pathological evaluation results showed that fucoidan could alleviate ballooning degeneration and cell necrosis in NAC NASH models, and could reduce lipid accumulation in liver cells and activation of stellate cell. Meanwhile, the immune assay for supernatant indicated that fucoidan could alleviate inflammation levels and collagen secretion in the NAC NASH models. The detection of reactive oxygen species (ROS), total superoxide dismutase (T-SOD) and malondialdehyde (MDA) indicated that fucoidan could reduce oxidative stress levels in the NAC NASH models. Meanwhile, transcriptome sequencing results indicated that fucoidan was involved in pathways such as TGF-β signaling pathway, inflammatory response, and reactive oxygen species metabolism. Further analysis indicated that GSTA2 was a key upregulated gene in the NAC NASH models treated with fucoidan.
Conclusion: This study highlights the favorable properties of fucoidan for NASH, making fucoidan a promising candidate for NASH treatment.
PP-03-169
Vitamin E Improves Serum Markers and Histology in Adults with MASLD: Systematic Review and Meta-analysis
Nicholas Ming-Zher Chee, Ram Prasad Sinnanaidu and Wah Kheong Chan
University Of Malaya, Kuala Lumpur, Malaysia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background and aim: Multiple clinical trials have been conducted to study the potential benefits of vitamin E for the treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). This study aimed to assess the effect of vitamin E on serum markers of liver inflammation, specifically serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and histology, including resolution of metabolic dysfunction associated steatohepatitis (MASH), in adult patients with MASLD.
Methods: A systematic literature search on randomised controlled trials published in English was conducted using electronic databases. Standardized mean difference (SMD) and mean difference (MD) were used for continuous outcomes, while risk ratio (RR) was used for dichotomous outcomes, with corresponding 95% confidence interval (CI).
Results: A total of eight studies were included in the qualitative synthesis while seven studies were included in the meta-analysis. Vitamin E significantly reduced serum ALT and AST levels with SMD of -0.82 (95% CI, -1.13 to -0.51) and -0.68 (95% CI, -0.94 to -0.41), respectively. Vitamin E significantly reduced steatosis, lobular inflammation, and hepatocyte ballooning with a MD of -0.60 (95% CI, -0.83 to -0.37), -0.34 (95% CI, -0.53 to -0.16), -0.32 (95% CI, -0.53 to -0.12), and increased MASH resolution with a RR of 1.9 (95%CI, 1.20 to 3.02). However, vitamin E did not reduce fibrosis, with a MD of -0.23 (95% CI, -0.51 to 0.05).
Conclusion: Vitamin E resulted in significant improvement in serum markers of liver inflammation and histology in patients with MASLD.
PP-03-170
Clinical Ultrasound Diagnosis of NAFLD and Complication of Metabolic Syndrome: Relationship with RTE, Attenuation Imaging
Masahiko Sugano
Sugano Internal Medicine Clinic, Himeji-City, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Aim: NAFLD is the most abdominal ultrasonography examination cases in our clinic. Complicated fibrosis is important in relation to Metabolic Syndrome (MS). NAFLD has a high MS complication:20.7%. Among 1290NAFLD examples(2018-23), NAFLD Fibrosis Score (NFS): High(>0.676) MS merger is 40.4%, Intermediate: 24.4%, Low(<-1.455): 17.6%. Hepatic steatosis is also associated with MS, we examined the relation between ultrasonic fibrosis, steatosis evaluation and MS merger.
Matherial and Method: We examined 1290 NAFLD cases (56.5y.o.; M725:F566, BMI:24.0, ALT27.0) who performed abdominal ultrasound in 2018-23 (March-May). Focusing on Strain Imaging; Real-time Tissue Elastography (Liver Fibrosis Index:LFI) and Share Wave Measurement (Vs) and Attenuation Imaging (ATT) at ultrasonic examination and Fib4index (F4i), M2BPGI (M2) were used to predict MS merger.
Correlation between each Fibrosis Index: LFI between Vs(r=0.273)/ NFS(0.252)/ M2(0.179); LFI<1.58; Vs:1.17(mean)/ NFS:-2854/ M2:0.48, 1.58-2.03; 1.18/ -2.289/ 0.53, 2.03~2.40; 1.25/ -1.840/ 0.67, 2.40~2.86; 131/ -1.533/ 0.76, >2.86; 1.50/ -1.058/ 0.90. F4i between LFI (r=0.129)/ Vs(0.227)/ M2(0.590)/ NFS(0.724);Table.
Study of MS Merger rate: LFI; <1.60 (56 cases): MS 1.8%, 1.60-1.80 (98):4.1%, 1.80-2.00 (170):4.1%, 2.00-2.20 (243):10.7%, 2.20-2.40 (267):16.9%, 2.40-2.60 (214):28.5%, 2.60-2.80 (129):43.4%, >2.80 (65):63.7%. MS cut off is considered as LFI>2.4 (sensitivity 0.69, specificity 0.74). The difference is not noticeable in Vs: (>1.25:MS25.5%; <1.25:16.1), F4i; (Low:22.5%, High:21.9). ATT:<0.62 (450):11.1%, 0.62-0.66 (184):17.9, 0.67-0.72 (190):24.2, >0.73 (206):34.5. MS C.O. is 0.67 (sensitivity 0.54, specificity 0.61).
Conlusion: LFI can numerically estimate the MS merger of NAFLD (above 2.4). For serum markers NFS was useful. In NAFLD cases, MS should be searched for with a focus on LFI and reference to Vs, NFS, and ATT.
PP-03-171
Does name matter? unraveling the implications of nafld to mafld then masld
Erika Johanna Tañada-Escanlar and Stephen Wong
University of Santo Tomas Hospital, Manila, Philippines
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: The second-most recent change in steatosis nomenclature required 2/3 weight-metabolic criteria to be met and may lead to missed diagnosis in patients who develop weight-metabolic abnormalities later on. The metabolic-dysfunction-associated steatotic liver disease (MASLD) criteria requires only one criterion and may mitigate this. We aimed to characterize our steatotic patients into: 1.) nonalcoholic fatty liver disease (NAFLD) only; 2.) MASLD only; 3.) MAFLD only ; 4.) MAFLD-MASLD.
Materials and methods: A cross-sectional analytical retrospective study reviewed 663 NAFLD patients with steatotic liver on ultrasound in a Hepatology clinic database from August 2007-July 2017 were included. Demographic, laboratories, AST/Platelet ratio (APRI), FIB4, and NAFLD fibrosis score (NFS) were compared between the groups.
Results: The 663 steatotic patients were categorized into: 4(0.9%) NAFLD, 8 (1.2%) MASLD only, 325(49%) and 651(98.2% ) belongs to MAFLD-MASLD overlap. All MAFLD-MASLD diagnosed patients are diabetic while none of the MASLD and NAFLD groups are diabetic. More patients from the MASLD and NAFLD groups have hepatitis B than the MAFLD-MASLD group. On the other hand, more MAFLD-MASLD patients have hypertension (74.0%), dyslipidemia (83.1%), HCC (6.0%), cirrhosis (4.5%), and CAD/CVD (17.2%) than the other two groups. They all have high fibrosis risk with 14.6% likely to have advanced fibrosis.
Conclusions: Majority (98.2%) of patients with steatosis on ultrasound meet MAFLD MASLD criteria which means that changing nomenclature has little difference on epidemiology. Additional longitudinal studies are needed to further illuminate on the implications of the terminology changes.
PP-03-172
Liver Stiffness in Metabolic-Associated Fatty Liver Disease With and Without Hepatitis: A Single Center Experience
1Department of Internal Medicine, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia;2Department of Anatomy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia;3Department of Internal Medicine, Gastroenterology and Hepatology Division Pantai Indah Kapuk Hospital, Jakarta, Indonesia;4Department of Radiology Pantai Indah Kapuk Hospital Jakarta Indonesia, North Jakarta, Indonesia;5Department of Internal Medicine, Cardiology and Vascular Division Pantai Indah Kapuk Hospital, Jakarta, Indonesia;6Division of Hepatobiliary, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Magnetic Resonance Elastography (MRE) is an advanced imaging technique used to assess fat accumulation and fibrosis in the liver, particularly in Metabolic-Associated Fatty Liver Disease (MAFLD). Hepatitis infection may contribute to the progression of liver fibrosis. This study aims to explore the correlation between liver stiffness in MAFLD patients with and without hepatitis infection.
Material and Methods: This retrospective cross-sectional study included 46 patients with clinically diagnosed fatty liver who underwent MRE examination between 2021 and 2022. The study evaluated the correlation between baseline variables, including hepatitis infection, and liver stiffness.
Results: Liver stiffness ranged from 1.45-7.80 kPa, with a geometric mean of 3.26 kPa and a coefficient of variation of 40.6%. No significant correlation was found between hepatitis infection and liver stiffness in MAFLD patients (p = 0.174). However, liver stiffness showed significant correlations with age (p = 0.047, r = 0.295), fibrosis markers (FIB-4 score, p < 0.001, r = 0.720; platelets, p < 0.001, r = -0.559), and indicators of hepatocellular damage (SGOT, p < 0.001, r = 0.615; GGT, p = 0.002, r = 0.533; ALP, p = 0.002, r = 0.573). Additionally, metabolic markers related to glucose control (HbA1c, p = 0.015, r = 0.501) and fat metabolism (total cholesterol levels, p = 0.011, r = -0.519) were correlated with liver stiffness.
Conclusion: Hepatitis infection was not correlated with liver stiffness in MAFLD patients. In Indonesian fatty liver patients, liver stiffness was significantly correlated with age, liver fibrosis markers, hepatocellular damage, and metabolic dysfunction.
PP-03-173
Impact of Glucagon-like Peptide-1 Agonists on MASH/ MASLD: Meta-Analysis of 14 Studies
Daniel Yi Nin Tung1, Etham Tham2, Pojsakorn Danpanichkul3, Jieling Xiao2 and Mark Muthiah1
1Division of Gastroenterology and Hepatology, Department of Medicine, National University Hospital, Singapore, Singapore;2Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore;3Department of Internal Medicine, Chiang Mai University, Chiang Mai, Thailand
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: This study aimed to evaluate the impact of GLP-1 agonists on patients with MASH/ MASLD via a systematic review and meta-analytic approach.
Materials and methods: Medline and Embase were searched for studies which were selected based on evaluation of the effects of GLP-1 agonists on outcomes such as resolution of MASH, liver fat content, fibrosis-4 index, fibrosis, weight, transaminases, cholesterol and triglycerides. Data was analysed to obtain odds ratio or standardised mean difference (MD) comparing use of GLP-1 to patients without GLP-1 usage.
Results: A total of 14 articles were included in the overall analysis. Patients treated with GLP-1 agonists had lower liver fat content (MD -3.82 CI -5.4 to -2.23; p<0.01), lower triglyceride levels (MD -39.68, CI -61.63 to -17.73; p<0.01), lower total cholesterol levels (MD -18.79, CI -35.19 to -2.39; p<0.01), lower LDL levels (MD -6.73, CI -11.45 to -2; p<0.01), lower HbA1c (MD -1.28, CI -1.66 to -0.91). They also had higher rates of resolution of MASH (OR 4.95 CI 3.6 to 6.82; p <0.01) and 1 or more point reduction in fibrosis with no worsening of MASH (OR 1.76, CI 1.29 to 2.42; p <0.01).
PP-03-174
Psyllium fiber improves hangovers and inflammatory liver injury by inhibiting intestinal drinking
Keungmo Yang1, Beom Sun Chung2 and Tom Ryu3
1The Catholic University of Korea, Seoul, South Korea;2Yonsei University Wonju College of Medicine, Wonju, South Korea;3Soonchunhyang University College of Medicine, Seoul, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Excessive alcohol intake often results in hangovers and inflammatory liver damage, posing a significant health concern. Current treatment options for hangovers are still insufficient, highlighting the urgent need for new therapeutic approaches. Psyllium fiber (PF) is well-known for its gastrointestinal benefits, but its effect on hangovers is less explored. We utilized a mouse model with a single binge drinking (4 g/kg) to induce hangover and inflammatory liver injury. Intestine and liver injury were serologically and histologically estimated. Hangover symptoms were assessed using cylinder and footprint tests to objectively quantify hangover symptoms in mice. Binge drinking significantly activated alcohol-metabolizing enzymes in the small intestine and liver, leading to inflammatory damage. Concurrently, there was a rise in alcohol metabolites such as acetaldehyde and acetone, which exhibited a positive correlation with hangover symptoms in mice. Interestingly, the oral administration of PF (100 mg/kg) alongside alcohol consumption significantly reduced the activity of these enzymes and lowered the levels of alcohol metabolites. Mice treated with PF exhibited a considerable improvement in hangover symptoms and a reduction in hepatic inflammation, compared to control groups. Furthermore, in vitro experiments using HepG2 cell lines and semipermeable membranes demonstrated that PF effectively inhibits alcohol absorption into the body. In conclusion, PF demonstrates a potential protective effect against alcohol-induced hangover and liver injury by inhibiting the absorption of alcohol and lowering hangover-related alcohol metabolites. This study suggests that PF could serve as an effective therapeutic option for mitigating the adverse effects of excessive alcohol consumption.
PP-03-175
The prognostic impact of psychiatric intervention on alcohol-associated liver disease: the UK Biobank cohort study
Keungmo Yang1, Sunghwan Kim1, Hyun Yang1, Bumseok Jeong2, Hyun Kook Lim1 and Si Hyun Bae1
1The Catholic University of Korea, Seoul, South Korea;2Korea Advanced Institute for Science and Technology, Daejeon, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background/Aims: Alcohol-associated liver disease (ALD) is a public health concern. ALD patients often have psychiatric comorbidities, but the effects of psychiatric interventions on ALD are not well-established. This study explores the prognostic impact of psychiatric intervention on ALD within UK Biobank cohort.
Methods: This prospective study included a total of 502,370 participants. Psychiatric intervention was defined by a consultation with psychiatrists during hospitalization or a history of medication related to alcohol use disorder and psychiatric comorbidities. Survival analysis was conducted, incorporating propensity score matching (PSM) and doubly robust (DR) estimation, to precisely assess the impact of psychiatric intervention.
Results: Among 2,417 ALD patients in the final analysis, those with F10 codes had poorer survival outcomes. Psychiatric intervention significantly improved the outcomes of both all-cause and liver-related mortality and reduced the incidence of liver cirrhosis. In subgroup analyses or 2-year landmark analyses, psychiatric intervention consistently showed a survival benefit in ALD patients. In the multivariate analysis, psychiatric intervention was identified as a favorable prognostic factor (hazard ratio, 0.780; P = 0.002 after PSM). Furthermore, the average treatment effects from DR estimation provided detailed insights into the impact of psychiatric intervention (2.9 years, P < 0.001 after PSM).
Conclusions: This study demonstrates the favorable effect of psychiatric intervention in ALD patients with psychiatric comorbidities. These findings emphasize the importance of integrated management for ALD patients to address both their medical and psychiatric aspects. Therefore, we suggest the potential benefits of early psychiatric interventions in improving survival outcomes in ALD.
PP-03-176
Frailty, Genetic Susceptibility and Non-alcoholic Fatty Liver Disease: from Two Large Cohorts and Genetic Analyses
Han Zhang1, Lingyi Li1, Lijun Zhang1, Zheng Li2, Yuying Ma1, Wentao Huang1, Ruijie Zeng1, Dongling Luo1, Yanjun Wu1, Meijun Meng1, Felix W Leung3, Chongyang Duan4, Weihong Sha1 and Hao Chen1
1Guangdong Provincial People's Hospital, Guangzhou, Guangdong Province, China;2Shantou University Medical College, Shantou, Guangdong Province, China;3University of California Los Angeles, Los Angeles, United States of America;4Southern Medical University, Guangzhou, Guangdong Province, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To evaluate the association, causality and genetic correlation between frailty and non-alcoholic fatty liver disease (NAFLD).
Materials and Methods: UK Biobank and the National Health and Nutrition Examination Survey (NHANES) respectively employed Cox proportional hazard regression models and logistic regression models to explore the role of frailty in NAFLD. Frailty Index (FI) and Frailty Phenotype (FP) were used to assess the frailty status. The combined effects of genetic susceptibility were explored using the polygenic risk score (PRS). Mendelian Randomization, Linkage Disequilibrium Score Regression (LDSC), Genetic Covariance Analyzer (GNOVA) and Heritability Estimation from Summary Statistics (ρ-HESS) were used to explore the causal association and genetic correlation.
Results: In the FP analysis using UK biobank, pre-frail (hazard ratio [HR] 1.48; 95% confidence intervals [CI] 1.38-1.58; P<0.001) and frail individuals (HR 2.10; [95% CI 1.92-2.30]; P<0.001) had increased risks of NAFLD. In the FI, the risk of NAFLD increased in individuals under pre-frailty (HR 1.93; [95% CI 1.80-2.06]; P<0.001) and frailty (HR 3.50; [95% CI 3.24-3.77]; P<0.001). Similar results were obtained in the NHANES. Subgroup analysis based on PRS showed even pre-frail/frail people with low genetic susceptibility were also at risk for NAFLD. There was significant causality (OR 2.00; [95% CI 1.40-2.86]; P<0.001) and positive genetic correlations (LDSC: rg =0.576, P<0.001; GNOVA: rg =0.777, P<0.001; ρ-HESS: rg =0.828) between FP and NAFLD.
Conclusion: Frailty greatly increases the risk of developing NAFLD, even in individuals with lower genetic risk. There was a significant causal and positive genetic correlation between FP and NAFLD.
PP-03-177
The association between triglyceride-glucose index and its combination with obesity indicators and MAFLD: NHANES 2017-2020
Jiayin Yu2, Yutong Bai2, Bo Wang1, Yongqiang Xiong1 and Shu Zhang1
1The Second Affiliated hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;2Xi’an Jiaotong University, Xi’an, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: The relationship between the triglyceride-glucose (TyG) index and TyG combined with indicators of obesity and MAFLD has been less well defined.
Methods: This cross-sectional study included 2007 adults from the National Health and Nutrition Examination Survey (NHANES) 2017-2020.Liner regression, binary logistic regression analyses, and receiver operating characteristic (ROC) were used to analyze the relationship between TyG and its combined obesity-related indicators and MAFLD.
Results: TyG, WHtR, and TyG-WHtR were significantly and positively associated with controlled attenuation parameter and MAFLD (TyG: OR 4.269, 95%CI 3.594-5.071, WHtR: OR 3.256, 95%CI 2.871-3.692, TyG-WHtR: OR 3.843, 95%CI 3.372-4.379). ROC curves showed that these three indicators were all related to MAFLD, TyG-WHtR had more robust diagnostic efficacy than TyG and WHtR (95%CI 0.795-0.832).
Conclusions: TyG and TyG-WtHR enhance diagnostic efficacy of MAFLD. TyG-WtHR are expected to become more effective metrics for identifying populations at early risk of MAFLD.
PP-03-178
WY14643, a agonist of PPARα, attenuates HFD-fed AIH via inhibiting JNK signaling pathway
Cuijuan Zhao and Lu Zhou
Department Of Gastroenterology And Hepatology, Tianjin Medical University General Hospital, Tianjin, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: The regulation molecular mechanism of HFD on the occurrence and development of AIH by PPAR α and JNK signaling pathway.
Materials and methods: Mice were fed for 16 weeks either normal chow diet (ND) or high fat diet (HFD). Mice were intravenously administered Con A (20 mg/kg) to induce AIH. GW6471 (20 mg/kg) or WY14643 (6 mg/kg) was injected intraperitoneally for 3days. The mice and liver and spleen tissues were weighted. The histopathological analysis was performed by HE staining.The mRNA and protein levels were detected using RT-qPCR and Western blotting.
Results: HFD could exacerbate the ConA-induced AIH, including liver steatosis and inflammation. Compared with other groups, necroptosis and apoptosis-related proteins, which were expressed at abnormally high levels in liver tissues of HFD+ConA group, were significantly up-regulated by HFD treatment. Therefore, HFD could promote the necroptosis and apoptosis in AIH mice. Meanwhile, HFD could inhibit the expression of PPARα and activate the JNK signaling pathway in AIH mice. Furthermore, PPARα inhibitor GW6471 could further aggravate the necroptosis and apoptosis of AIH mice and PPARα agonist WY14643 could alleviate the deterioration of HFD in AIH mice. Importantly, PPARα agonist WY14643 could further alleviate the necroptosis and apoptosis of AIH mice through inhibiting the JNK signaling pathway.
Conclusion: PPARα could have efficacy on inhibiting HFD-fed AIH progression by inhibiting the necroptosis and apoptosis of hepatocyte and the lipid metabolism abnormity of liver. Further molecular mechanism study found that WY14643 could upregulate the PPARα and inhibit the function of activating JNK signaling pathway.
PP-03-179
Estimation of fibrosis in autoimmune hepatitis with Shear wave Elastography
Raja Taha Yaseen Khan, Vijesh Kumar, Abbas Ali Tasneem and Nasir Hassan Luck
Sindh Institute Of Urology And Transplantation, Karachi, Pakistan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: This study aimed to determine the association between histologic liver fibrosis and Shear Wave Elastography (SWE) measurements of liver stiffness in patients with autoimmune hepatitis (AIH).
Material and Methods: Conducted at the Hepatogastroenterology unit at Sindh Institute of Urology and Transplantation Karachi, this cross-sectional study included all diagnosed AIH patients who had received a liver biopsy and undergone SWE from March 2022 to December 2023. Patients with other liver diseases were excluded. Liver fibrosis was evaluated using the Metavir scoring system. SWE measurements, performed by an experienced radiologist, required patients to fast for at least 4 hours before the examination. SWE results were compared with histologic findings from liver biopsies. Data were analyzed using SPSS 23.0.
Results: The study included 162 patients (91 males, 71 females) with a mean age of 35.8 ± 16.6 years. ANA was positive in 123 patients, ASMA in 47, and anti-LKM in 4. SWE detected fibrosis stages as F1 in 50 (30.9%), F2 in 44 (27.1%), F3 in 40 (24.7%), and F4 in 28 (17.3%) patients. Liver biopsies revealed severe fibrosis in 40 patients and cirrhosis in 30. SWE measurements significantly correlated with Metavir scores from liver biopsies (p < 0.001), with higher fibrosis stages (F3-F4) showing higher SWE measurements.
Conclusion: SWE is a reliable non-invasive method for estimating liver fibrosis in AIH patients, significantly correlating with histologic findings. This technique can potentially reduce the need for liver biopsies, minimizing associated risks and improving patient care.
PP-03-180
A Case of Sustained Virological Response of Chronic Hepatitis E With Ribavirin and Zinc Therapy
Huanghuan Li1, Yiying Pei2 and Rajneesh Kumar2
1Sengkang General Hospital, Singapore, Singapore;2Singapore General Hospital, Singapore, Singapore
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Hepatitis E virus (HEV) is a one of the leading causes of hepatitis worldwide. Here, we report a case of refractory chronic hepatitis E achieving sustained virological suppression with 2 months of dual therapy of ribavirin and zinc.
72 year old Chinese female was seen in 2016 for AST of 325 U/L and ALT of 225 U/L. She has background of Evan Syndrome with immune thrombocytopenia (ITP) and Systemic Lupus Erythematous (SLE) on high dose prednisolone since 2012. Attempt was made to reduce her prednisolone dose, followed by ribavirin 400mg daily for 3 months in view of persistence of HEV viral load. Undetectable HEV and normal transaminases were noted thereafter. Seven months after stopping ribavirin, AST and ALT rose to 159 IU/ml and 129 IU/ml with detectable HEV. He was restarted on ribavirin. HEV RNA persisted around 3.4 – 7 log for the next 6 years. Fibroscan showed stage 1 fibrosis in 2022 and 2023. Zinc 50mg daily was added in Nov 2023 after 6 years of maintenance ribavirin therapy. HEV RNA was undetectable in PCR since January 2024 with normal transaminases.
There are currently no established treatment options for chronic HEV patients who could not achieve sustained virological response in spite of reduction in immunosuppressant and ribavirin use. In our case report, zinc 50mg OM was added to ribavirin therapy for 2 months. Chronic HEV was suppressed. This is one of the first few case reports on use of zinc and ribavirin dual therapy in achieving chronic HEV suppression.
PP-03-181
Performance of noninvasive seromarkers in predicting liver fibrosis in mafld patients
Annisa Zahra Mufida1, Nurike Setiyari Mudjari2 and Titong Sugihartono1
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objective: To compare Fibrosis-4 (FIB-4), AST to platelets ratio index (APRI) and NAFLD fibrosis score (NFS) to FibroScan for the assessment of hepatic fibrosis in MAFLD patients.
Material and Method: A cross-sectional study was conducted on adult T2DM patients at Dr. Soetomo Hospital, Surabaya, Indonesia. Subjects underwent laboratory and transient elastography (TE) examination. The liver stiffness measurement (LSM) and controlled attenuation parameter (CAP) determined using FibroScan (Echosense). Patient with history of alcohol, hepatitis B or C, autoimmune hepatitis, and steatogenic drugs were excluded. The variables include the APRI Score, FIB-4, NFS, and FibroScan Score. Analysis was conducted using Pearson correlation, independent t test, and ROC curve analysis.
Results: There were 80 patients in this study with an average age of 54.83 years and female patients (55.0%). Independent test results showed that there were significant differences in APRI Score, FIB-4, and NFS between patients who did not experience fibrosis (F0 and F1) and patients who experienced fibrosis (F2, F3, and F4). The results showed that NFS could diagnose liver fibrosis (F2, F3, and F4) compared to APRI Score and FIB-4 with AUC 0.693, optimal cut off -1.265, sensitivity 95% and specificity 85%. The APRI Score index obtained an AUC of 0.685, optimal cut off 0.155, sensitivity 80%, and specificity 48.3%. Meanwhile, the FIB-4 index obtained an AUC of 0.693, an optimal cutoff of 0.655, a sensitivity of 95% and a specificity of 85%.
Conclusion: NFS is the best index for diagnosing liver fibrosis compared to APRI Score and FIB-4.
PP-03-182
Risk factors for primary biliary cholangitis combined with Sjogren's syndrome: a cohort of patients from China
Yu Peng and Xiaowei Liu
Xiangya Hospital, Central South University, Changsha, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: To know about the risk factors of primary biliary cholangitis (PBC) combined with Sjogren's syndrome in the Chinese patients.
Materials and Methods: Retrospective analysis of data from resident patients diagnosed with PBC at Xiangya Hospital from January 2017 to December 2023 was performed. The patients were divided into groups combined with or without Sjogren's syndrome. A total of 42 items from clinical features to laboratory tests were included in the statistical analysis. Data were analyzed using Chi-squared test, Fisher exact test, Logistic regression.
Results: 93 consenting PBC combined with Sjogren's syndrome patients, were compared with 93 PBC patients matched for age. Among the patients, 21(11.3%) male and 165(88.7%) female. Chi-squared test showed that PBC combined with Sjogren's syndrome was related to the following factors: 1) gender (p<0.05); 2) the level of extractable nuclear antigen (ENA) (p<0.001); 3)c. the level of total bile acid (TBA) (p<0.05); 4) the level of prothrombin time (PT) (p<0.05); 5) the level of international normalized ratio (INR) (p<0.005); 6) the level of activated partial thromboplastin time (APPT) (p<0.05). The independent risk factors for PBC combined with Sjogren's syndrome was the level of INR (odds ratio (OR): 5.683, 95% confidence interval (CI): 1.401-31.719, p=0.017).
Conclusion: This pilot study suggests that the independent risk factors for PBC combined with Sjogren's syndrome in Chinese patients was the level of INR. Indeed, the pathogenic mechanisms, clinical features, and optimal therapeutic approaches for PBC and Sjogren's syndrome are not yet fully defined. This issue should be systematically investigated.
PP-03-183
Unveiling the Enigma, case of Mosaic Alpers Syndrome
Vujwal Roy
Aster Medcity, Kochi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Alpers-Huttenlocher syndrome, first received attention from Alpers in 1931 and was further described with its hepatic manifestation by Huttenlocher et al. It is a rare mitochondrial disease characterized by its classic triad of refractory seizures, psychomotor regression, and hepatopathy.
Case discussion: 38 years male, known Decompensated Cirrhosis of Liver presented to our institute with c/o progressive abdominal distension. Ascitic Fluid study was suggestive of Spontaneous Bacterial peritonitis and was started on Antibiotics and other supportive measures.
His past history revealed history of refractory seizures, hemineglect, hemianopia, Sensorineural hearing loss and hemiparesis at the age of 21 years, for which he was started on dual anticonvulsants elsewhere. MRI Brain showed hyperintense gyri suggestive of Mitochondrial disease. CSF Analysis showed lymphocytic pleocytosis with high protein, viral etiologies were ruled out. He also had high lactate levels and a diagnosis of MELAS was made and he was started on oral steroids. He persisted to have intermittent episodes of seizures for which another anticonvulsant was added. He developed pedal edema and was diagnosed to have cirrhosis at 31 years of age.
Discussion: In current admission, considering his liver disease, encephalopathy, seizures a clinical diagnosis of Mosaic Alper Syndrome was made. He has been counselled for liver transplantation and is currently awaiting donor. Alpers-Huttenlocher syndrome is a mitochondrial disorder polymerase gamma(POLG) mutations, that decrease mitochondrial DNA replication. Ultimately, POLG dysfunction induces a progressive depletion of mitochondrial DNA and evolving organ dysfunction. Mosaic Alpers is a milder form or variant of classic Alpers syndrome presentation
PP-03-184
Pyogenic hepar abscess et causa mtb and salmonella paratyphi serotype a: a case report
Arlyando Saragih
Hermina Jatinegara, Jakarta Timur, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: A liver abscess is defined as a pus-filled mass in the liver. The incidence of liver abscess is typically low, the annual incidence rate is about 2.3 cases per 100,000 people. It is essential to early detect and manage these lesions, since there is a significant mortality risk in untreated patients. One of the most common forms of liver abscess is pyogenic liver abscess.
Case Description: We report a case of liver abscess that occurred in segment IV of the liver, with non-specific complaints, with culture results showing 2 causative germs, namely Mycobacterium Tuberculosis and Salmonella Paratyphi Serotype A. The patient came with chief complaints of middle abdominal pain accompanied by nausea and intermittent fever. Radiological examination in the form of CT-Scan and USG confirmed the diagnosis of liver abscess in the fourth segment. Aspiration abscess drainage combined with antibiotic therapy and anti tuberculosis is a safe and effective therapeutic approach for this case.
Discussion: Liver abscess has a low incidence rate, and not a very common case. This case shows that suspicion is necessary for the diagnosis of liver abscess in a young woman with good socio-economic and nutritional conditions, and the importance of carrying out a complete examination of the specimen to determine the exact etiology.
PP-03-185
Clinical presentation and outcome of Hepatic Langerhans cell histiocytosis: Two-decade experience from a tertiary center
Jayendra Seetharaman, Arul Premanand Lionel, Leni Grace Mathew, Mercy Pulipatti and Leenath Thomas
Christian Medical College, Vellore, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Langerhans cell Histiocytosis (LCH) is a rare disorder of children characterised by neoplastic dissemination of CD1a and CD207 (langerin) positive cells. The liver involvement of LCH happens in isolation or as a part of multisystemic disease. The literature on clinical presentations and outcome following treatment are scarce. The study aims to assess the presentation of hepatic LCH and liver outcomes following treatment in children
Methods: Children < 18 years, diagnosed LCH between 2003-2023 with any one a. Presence of hepatomegaly/ Jaundice/ ascites b. deranged liver function tests (LFT) c. abdominal imaging suggesting liver involvement were included. The presentations and outcomes following treatment were retrospectively analysed.
Results: 66 children [mean age (21.83 ± 12.63) months, 48 boys] diagnosed hepatic LCH were included. The duration of symptoms was 5.174 ± 3.75 months. Most common presentations were fever (93.9%) followed by abdominal distention (80.3%), anemia (75.8%) and jaundice (39.4%). 65 (98.4%) underwent ultrasonogram (USG) of which 22 (33.3%) underwent computed tomography (CT) abdomen and 10 underwent liver biopsies. 5 (7.6%) had isolated liver involvement. 56 children had at least one follow-up (30.09 ± 32.53 months). 20 were cured, 30 has persistent LCH, 10 died either on or after treatment. 31 (55.3%) children had liver dysfunction (jaundice, portal hypertension, sclerosing cholangitis) during last follow-up. Characteristics of children with good and bad liver outcomes were compared (Table 1).
Conclusion: Children with hepatic LCH has high mortality and morbidity. Presence of jaundice, anaemia, elevated transaminases and abnormal parenchymal architecture at presentation predicts poor liver outcome.
PP-03-186
Unique case of Budd Chiari Syndrome with Sagittal sinus Thrombosis By Dr Zafar
Athesham Zafar
Walsall Manor Hospital, Walsall, United Kingdom
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Budd-Chiari syndrome (BCS) is a rare but serious condition characterized by the obstruction of hepatic venous outflow, leading to liver damage. This blockage can result from thrombosis, compression, or other forms of obstruction in the hepatic veins or the inferior vena cava. Early diagnosis and treatment are crucial.
Case Description: 28 year old female who attended her doctor due to 2 months of lethargy and abdominal pain. An ultrasound was performed that showed gross ascites. (CT) was arranged that showed a heterogenous liver parenchyma with faintly visible hepatic veins. It therefore was decided to transfer her to our liver unit at Birmingham.
She also complained of headache and blurring of her vision. Fundoscopy was performed it showed papilledema.
CT triple phase showed thready left hepatic artery and collapsed hepatic veins. Histology from liver biopsy found severe venous outflow obstruction with confluent perivenular necrosis, in keeping with the diagnosis of Budd Chiari Syndrome.
CT Venogram Confirmed Sagittal Sinus Thrombosis. She was prothrombotic.
Accordingly, the patient was started on low molecular weight (LMW) heparin and underwent a Transjugular intrahepatic portosystemic shunt (TIPPS) without complication.
The cause of her prothrombotic state remained uncertain and she lacked obvious risk factors. Flow cytometry identified a substantial clone of Paroxysmal Nocturnal Haemoglobinuria. She was started on Eculizumab for treatment of PNH.
Discussion: Budd-Chiari syndrome requires a high index of suspicion for early diagnosis. Management strategies for BCS include anticoagulation and Liver transplantation may be considered in cases of severe liver failure.
PP-03-187
Littoral Cell Angioma as a Cause of Non Cirrhotic Portal Hypertension with Severe Splenomegaly
Donald Boy P Purba, Budi Widodo and Titong Sugihartono
Division of Gastroentero-Hepatology, Departement of Internal Medicine, Faculty of Medicine, Dr. Soetomo General Academic Hospital, Universitas Airlangga, Surabaya, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Littoral Cell Angioma (LCA) is a rare primary tumor of the spleen arises from the lining cells of the trabeculated mesh in the reticuloendothelial system. Only 150 cases reported in the medical literature, but none of them from Indonesia.
Case Illustration: A 28year old male presented with a three month history of upper left abdominal pain, abdominal distension, nausea, decreased appetite, jaundice, and dark urine. Physical examination revealed jaundice, hepatosplenomegaly, and ascites. Laboratory tests showed leukocytosis, thrombocytosis, direct hyperbilirubinemia, and elevated transaminases. Abdominal ultrasound revealed hepatosplenomegaly, ascites, and multiple nodules on the spleen. Contrast enhanced CT scan showed hepatomegaly without cirrhosis signs, splenomegaly, ascites, and perisplenic sub lymph paraaortic portocaval lymphadenopathy. Endoscopy revealed grade III esophageal varices with bleeding signs, antral varices, moderate portal hypertension gastroduodenopathy confirming noncirrhotic portal hypertension. Spleen FNAB showed CD3 positive endothelial cells lining capillary blood vessels, with CD30 and CD34 negativity, consistent LCA. The patient was treated furosemide 40 mg, propranolol 40 mg, spironolactone 100 mg daily. splenorenal shunt surgery was performed to reduce portal pressure.
Discussion: LCA can occur across various age groups and genders. Clinical manifestations include splenomegaly of unknown etiology, accompanied by abdominal pain, sometimes incidentally discovered as single or multiple lesions on the spleen. Characteristic findings on CT, MRI, ultrasound, and Tc99m RBC scan correlate with histopathological and immunohistochemical features. Definitive diagnosis of LCA relies on morphological and immunohistochemical features distinguishing it from other vascular lesions of the spleen.
PP-03-188
Updated Burden of Stomach Cancer in Hong Kong Chinese: a time-trend analysis
Junjie Huang, Yat Ching Fung, Chenwen Zhong, Sze Chai Chan and Martin Wong
The Chinese University Of Hong Kong, Sha Tin, Hong Kong
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background and Aims: This study aimed to provide a comprehensive analysis of the disease burden, incidence trends, and mortality trends of stomach cancer.
Methodology: Data on new cases and deaths related to stomach cancer were obtained from the Hong Kong Cancer Registry. Age-standardized rates (ASRs) of incidence and mortality were calculated. Joinpoint regression analysis was conducted to evaluate the Annual Average Percentage Change (AAPC).
Results: In 2020, there were 1,197 newly reported stomach cancer cases in Hong Kong, with an ASR of 6.9. The incidence was higher among males (ASR=8.7) compared to females (ASR=5.3), and remarkably higher in the older population aged over 50 years (ASR=29.3) compared to the younger population (ASR=1.3). There were 613 newly reported deaths, with an ASR of 3.2. Similarly, the mortality was higher in males (ASR=4.1) than in females (ASR=2.4), and substantially higher in the older population (ASR=13.8) compared to the younger population (ASR=0.4). Trend analysis revealed a decreasing mortality trend for both sexes and age groups, while no significant increase or decrease was observed in the overall incidence trend. Notably, the male population was the only group with a significant decline in both incidence (AAPC: -1.5, 95% CI: -2.3, -0.6, p=0.005) and mortality (AAPC: -3.4, 95% CI: -5.8, -1.0, p=0.006) trends.
Conclusion: The findings of this population-based study suggest that while the mortality of stomach cancer in Hong Kong is decreasing, the incidence remains relatively stable. Continued efforts to promote healthy dietary habits could be crucial in lowering the overall incidence of stomach cancer.
PP-03-189
Endoscopic Ultrasonographic Diagnosis of Pancreatic Tuberculosis: A Case Report
Sri Angraeni, Saskia Aziza Nusyirwan and Hasan Maulahela
Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Departement of Internal Medicine, Faculty of Medicine, University of Indonesia, Ciptomangunkusumo Hospital, Jakarta, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: Despite the high prevalence of tuberculosis (TB) in developing countries, pancreatic TB remains a rare disease. Endoscopic ultrasound provides high resolution images of the pancreatic lesions as well as an opportunity to sample these lesions for cytological confirmation.
Case Presentation: A 23-year-old female patient came with chief complaint abdominal pain since 3 month ago, weight lost 4 kg in the last 3 months, and there was lump in the left side of the neck. Physical examination showed abdominal pain and left colli lymphadenopathy, HIV test was negative, chest x ray was normal. MRCP showed multiple loculated/ lobulated cysts in caput, uncinate and proximal of pancreatic body and multiple lymphadenopathy at para-aorta. Endoscopic ultrasound with fine-needle aspiration (EUS FNA) was performed and found peri-pancreatic lymphadenopathy, hypoechoic, with the clear boundary. We performed cytology and TB PCR test. The result of TB PCR was positive. Cytology examination from EUS FNA found granulomatous chronic inflammation, and cytology of left colli lymphadenopathy showed chronic lymphadenitis were suspected caused by tuberculosis.
The final diagnosis were pancreatic tuberculosis and tuberculous lymphadenitis. The patient is currently undergoing the 11th months out of the planned 12 months of standard treatment of anti tubercular therapy. Now the patient no longer experiences complaints of abdominal pain and lump in the neck, her weight has increased.
Discussion: Pancreatic tuberculosis is one of the rarest forms of tuberculosis and it presents with non- specific clinical manifestations. Regarding establishment of an accurate diagnosis, endoscopic ultrasound with fine-needle aspiration has an important role.
Rumah Sakit Umum Pusat Dr. M. Djamil Padang, Padang, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Ulcerative colitis with colorectal cancer has presented a series of clinical problems, with its poorer prognosis and higher mortality rate than sporadic colorectal carcinoma. Chronic inflammation is a significant contributor to the development of ulcerative colitis with colorectal carcinoma. In the management of ulcerative colitis, it can depend on the severity of the disease.
Case description: 62 year old female patient with bloody stools that had increased since 2 days ago. Diarrhea had occured since 6 months ago. Based on physical examination, BP was found to be 140/90 mmHg, anemic conjunctiva and epigastric pain. The results of supporting examinations showed that Hb was 9.3 gr/dl, a chest x-ray examination revealed cardiomegaly with a CRT > 60%. Colonoscopy showed severe ulcerative colitis with descending colon Ca, the examination was then followed by colon biopsy with results of colon carcinoma with ulcerative colitis. The patient received Mesalazine therapy 3x500 mg with prednisone 3x 10 mg. The patient also received a 1 unit PRC transfusion while the bleeding was still active. During treatment, the patient showed clinical improvement as indicated by the cessation of rectal bleeding and reduced frequency of diarrhea.
Discussion: Patients with ulcerative colitis have a two- to three-fold increased risk of developing colorectal cancer compared to the general population. Ulcerative colitis with colorectal cancer has poorer prognosis and higher mortality rate than sporadic colon carcinoma.
Celiac disease: variable presentations & social implications, data from a developing country
Talal Bhatti and Madeha Irfan and Fareed Muslim and Ali Sufyan
Szabmu, Islamabad, Pakistan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Intro: Celiac disease is an autoimmune enteropathy triggered by ingestion of gluten in genetically susceptible individuals. It has varied clinical presentations. Not much is known about presentation of this disease in Pakistani adults.
Methods: This is a cross sectional study conducted at a public sector tertiary care setup of Islamabad. Patients who were diagnosed cases of Celiac disease on the basis of serology and/or histopathology were interviewed via a structured questionnaire via non probability consecutive sampling technique. statistical analysis was performed IBM SPSS version 25.
Results: A total of 340 patients were enrolled in present study. Most predominant intestinal symptom was Diarrhea reported by 46.8% of participants followed by Abdominal pain/Bloating in 44.7% (Figure I). Most common Extra-intestinal features were Anemia reported by 51.5% of patients (Figure II). Of note constipation was reported by 21.5% and Stunted growth by 35.3% of patients. Most common utilized diagnostic modality was Serology in 62.9% of patients (Figure III). 86.8% of patients reported symptom resolution with Gluten free diet. This association was statistically significant. Patients who reported Abdominal pain/Bloating were significantly more likely to report improvement in symptoms following Gluten free diet (Figure IV). Challenges in Gluten free diet were availability issues reported by 37.5% followed by financial issues in 25%, social challenges in 19.2% and other issues including compliance and cross contamination by 40% (Figure V)
Conclusion: Celiac Disease has an extremely Variable Presentation in Pakistani patients. It can present with Classical Diarrhea and Non classical presentation with abdominal pain and bloating as well.
PP-03-258
Celiac disease- extra intestinal presentations; perspective from a developing country
Talal Bhatti and Fareed Muslim and Madeha Irfan and Ali Sufyan
Szabmu, Islamabad, Pakistan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Celiac disease (CD) is an immune mediated bowel disorder characterized by sensitivity to gluten. Diagnosis of celiac disease is made by history and clinical presentation compatible with CD, serological screening compatible with CD, histological findings, compatible with CD, obvious clinical and serological response to a gluten-free diet. Extra Intestinal manifestations in Celiac population in Pakistan in not well studied.
Methods: This is a cross sectional study conducted at a public sector tertiary care setup of Islamabad. Patients who were diagnosed cases of Celiac disease on the basis of serology and/or histopathology were interviewed via a structured questionnaire via non probability consecutive sampling technique. Age, gender, age of diagnosis, diagnostic modality, extra intestinal features, gluten free diet status response recorded while ensuring patient anonymity. Data was entered and statistical analysis was performed via IBM SPSS version 25.
Results: A total of 340 patients were enrolled in present study. Mean age of participants was 21.07 ± 12.99 years with predominantly female patients (62.9%). Most common Extra-intestinal feature was Anemia reported by 51.5% of patients (Figure I). Dermatological features and Malaise were significantly more common in patients with higher age. Anemia was significantly more likely in Female patients. Patients with stunted growth is significantly more likely to feel socially left out.
Conclusion: Celiac Disease can present with multiple extra intestinal features. Most common feature was Anemia. Extraintestinal manifestations may have gender predisposition as Anemia is significantly more likely in female gender and age as Dermatological features were more likely in relatively older patients.
PP-03-261
Capsule endoscopy: Unfolding the diagnostic dilemma
Jitender Koli
army hospital research and refferal, New Delhi, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Capsule Endoscopy: Unfolding The Diagnostic Dilemma
KOLI Jitender; DHIMAN Priyank
Army Hospital Research and Referral ,Delhi
Objective: The aim of this study was to reveal the usefulness of capsule endoscopy in diagnosing small bowel lesions in patients who presented with various indications in our Centre not diagnosed by conventional endoscopic techniques.
Material and Methods: We studied the capsule endoscopy reports of 75 patients who presented to our center in last 1 year for evaluation of obscure gastrointestinal bleed,iron deficiency anemia , unintentional weight loss and patients of abdominal pain failed to diagnosed by convention bidirectional endoscopy.
Out of the 75 patients , 61 patients were of obscure gastrointestinal bleeding, 10 of Iron deficiency anemia ,3 of abdominal pain and 1 of unintentional weight loss.
Results: In 30 patient we could not find anything and was reported as normal study.
7 out of 10 IDA patient,4 out of 4 abdominal pain,19 out of 61 obscure gastrointestinal bleed had normal study.
In 52 patient of obscure GI bleed 12 patients had duodenal and jejunal telengiectasias,6 had ileal telengiectasias,5 had small bowel erosions,4 had terminal ileal ulcers,5 had small bowel erosions,5 had jejunal ulcers,2 had jejunal polyp,2 had ascariasis and 1 had smalll bowel GVHD.
In 4 IDA patient 1 had ascariasis,2 had jejunal telengiectasia and 1 ileal ulcer.
Conclusion: Capsule endoscopy is very useful tool for the diagnosis of small bowel lesion leading to obscure gastrointestinal bleeding, anemia. Utility is limited if used in patients of unintentional weight loss.
PP-03-276
A mucosal prolapse syndrome with preoperative suspicion of cancer treated by endoscopic mucosal resection
Takanori Suganuma
Suganuma Hospital, Iida, Nagano, Japan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Conservative treatment, such as improving defecation habits, is the first choice for treatment of rectal mucosal prolapse syndrome (MPS), but in cases refractory to treatment, excision of the MPS itself is an option.
Case Description: 85 year old male. He has been bleeding hemorrhoids for a long time. Positive result of occult blood in stool at physical checkup. A total colonoscopy was performed by his previous physician. A 20 mm-sized SMT-like elevated lesion was found in contact with the dentate line in the rectum-anal canal region. The biopsy by the previous physician was Group 4, and the lesion was suspected to be a mucinous adenocarcinoma. Rectal examination revealed no mass and only a slight lump at 5 o'clock. Contrast-enhanced CT showed an area of abundant blood flow in the anal wall.
Cancer was suspected, but Miles surgery was too invasive and the patient was referred to our hospital. After a second lower endoscopy, NBI showed a Type 2B (JNET) structural pattern, and IDUS showed no evidence of deep invasion, so the patient underwent en bloc resection by EMR. Pathological examination showed varicocele formation across the dentate line, erosion and regeneration of the capsular epithelium, granulation of the stroma, and regenerative atypia of the epithelium, which was a finding of MPS associated with hemorrhoid. Hemorrhoidal bleeding improved.
Discussion: In this report, we describe a case of MPS in which cancer was suspected preoperatively and hemorrhoidal bleeding symptoms improved after endoscopic resection.
PP-03-288
Contrast-enhanced Endoscopic-ultrasound improves diagnostic performance of EUS-guided-tissue-acquisition in solid pancreatic masses arising in chronic pancreatitis
Institute of Gastrosciences and Liver diseases, Apollo Multispeciality Hospital, Kolkata, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Ancillary techniques for improving diagnostic accuracy of Endoscopic-ultrasound guided-tissue acquisition(EUS-TA) for solid pancreatic masses, are evolving. We aimed at evaluating role of contrast-enhanced EUS(CE-EUS) and EUS-Elastography(EUS-E) in improving diagnostic performances of EUS-TA for solid pancreatic masses.
Materials and Methods: Patients with solid pancreatic masses undergoing EUS-TA using either standard B-mode-EUS alone or CE-EUS guidance from July 2023 to June 2024 were analyzed. Outcome measures were i) comparison of diagnostic performance between two modalities in all solid pancreatic masses and ii) comparison of diagnostic performance in pancreatic mass with chronic pancreatitis. Strain ratio obtained by EUS-E was also recorded for masses where CE-EUS was used. Diagnostic performance of use of combined strain ratio and enhancement pattern was also analyzed.
Results: Out of total 94 patients, 62 underwent B-mode-EUS guided-TA and 32 underwent CE-EUS guided-TA.Overall for differential diagnosis, B-mode-EUS performed similar to CE-EUS. To diagnose malignant lesion in background of chronic pancreatitis, when compared to B-mode, CE-EUS had higher diagnostic accuracy(90.91%vs78.95%,p-0.0083), higher sensitivity (83.33 %vs50%,<0.0001) with similar specificity, similar positive predictive value and similar negative predictive value (table 1). Presence of combination of strain ratio above 6.24 and hypo-enhancement pattern on CE-EUS had sensitivity of 85% and specificity of 100% for predicting adenocarcinoma. When this combination was used in pancreatic masses with chronic pancreatitis, sensitivity increased to 100% for predicting adenocarcinoma.
Conclusion: Advanced EUS modalities including contrast-enhancement and elastography should be used while performing EUS-TA for pancreatic masses with chronic pancreatitis as it improves diagnostic performance over standard B-mode EUS.
PP-03-290
Alteration in Gut Microbiota after Colonoscopy: Proposed Mechanisms and the Role of Probiotic Interventions
Yong Sung Kim
Wonkwang University, Digestive Disease Research Institute, Seongnam, South Korea
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: Colonoscopy induces transient gastrointestinal symptoms and alterations in the gut microbiota. This study aims to review the alterations in the gut microbiota following colonoscopy, their possible mechanisms, and the effect of probiotics.
Materials and Methods: We conducted a thorough literature search in the PubMed database in this review. For studies investigating alterations in gut microbiota after colonoscopy, we used search terms: (colonoscopy AND (microbiota OR microbiome) AND (dysbiosis OR effect OR alter OR change)). To identify studies examining the effects of probiotic use before and after colonoscopy, we used the search terms: (colonoscopy AND (probiotics OR probiotic)). We did not restrict publication year, language, or study design. Studies were screened for relevance based on titles and abstracts, and duplicates were removed.
Results: Factors such as rapid colonic evacuation, increased osmolality, and mucus thinning caused by bowel preparation and exposure to oxygen during the procedure contribute to these alterations. Typically, the alterations revert to the baseline within a short time. However, their long-term implications remain unclear, necessitating further investigation. Split-dose bowel preparation and CO2 insufflation during the procedure result in fewer alterations in the gut microbiota. Probiotic administration immediately after colonoscopy shows promise in reducing alterations and gastrointestinal symptoms. However, the widespread use of probiotics remains controversial due to the transient nature of the symptoms and microbial alterations in the microbiota.
Conclusion: Colonoscopy induces transient dysbiosis. Probiotics may offer greater benefits to individuals with preexisting gastrointestinal symptoms. Thus, probiotic administration may be a viable option for selected patients.
PP-03-305
Colorectal cancer incidence and mortality in Mongolia, 2018–2022
Institute of medical sciences, Mongolian national university of medical sciences, Ulaanbaatar, Mongolia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: We aimed to update data on the morbidity and mortality rate of colorectal cancer (CRC) among the population of Mongolia by province between 2018 and 2022.
Materials and methods: This study was designed using a descriptive method. The data were collected from 21 general hospitals of provinces, 9 general hospitals of districts, and the National Cancer Center in 2018–2022. The incidence and mortality were calculated as mean annual numbers per 100,000 populations.
Results: In the country, a total of 1316 (634 male) new cases were diagnosed and 782 deaths were caused by CRC in the last 5 years (2018–2022). The incidence of CRC in the last 5 years was 7.9 per 100,000 populations, and the mortality rate was 4.7 per 100,000. The provinces of Orkhon (12), Khentii (11), and Central (10) reported the highest incidences of CRC, whereas the provinces of Sukhbaatar (6.9), Selenge (6.6), Dornod (6), and Darkhan-Uul (6) had the highest death rates (per 100,000 populations). The incidence of CRC didn’t differ statistically significantly between men and women. Additionally, the estimated incidence has grown dramatically with patients’ ages.
Conclusion: Our study presents evidence of a steadily increasing CRC incidence in Mongolia over the past five years. Therefore, it is necessary to determine the distribution of risk factors, learn from the experience of developed countries strategy for prevention, diagnosis, and treatment.
PP-03-312
Empirical Therapy Versus Susceptibility Testing-Guided Therapy for Refractory Helicobacter pylori Infection: A Randomized Controlled Trial
Jyh-Ming Liou, Mei-Jyh Chen, Chieh-Chang Chen and Ming-Shiang Wu
National Taiwan University Hospital, Taipei, Taiwan
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background and Aims: We aimed to evaluate whether optimized empirical therapy is non-inferior to susceptibility-guided therapy in treating refractory H. pylori infection.
Methods: We conducted a multicenter, open-label, randomized controlled trial in Taiwan. Eligible patients were allocated to receive either empirical guided therapy or susceptibility testing guided therapy. The MICs were determined using the agar dilution test. Study participants received levofloxacin-based quadruple, bismuth quadruple therapy, rifabutin-based or clarithromycin-based concomitant therapies based on previous medication history or susceptibility testing results. The status of H. pylori was determined using the 13C-UBT at least 6 weeks after completing eradication therapy. The pre-specified margin for non-inferiority was set at 9%.
Results: Among the 233 patients with refractory H. pylori infection who were randomly assigned and underwent post-eradication evaluation, the eradication rates for the empirical guided therapy group and the susceptibility testing guided therapy group were 82.6% (95% CI: 75.7%-89.5%) and 85.6% (95% CI: 79.3%-91.9%) in the ITT analysis (p = 0.533), respectively. In the PP analysis, the eradication rates were 82.5% (95% CI: 75.5%-89.4%) versus 87.1% (95% CI: 81.0%-93.2%) (p = 0.330). The difference in eradication rates between the empirical guided therapy and susceptibility testing guided therapy groups was -3.0% (95% CI: -11.0% to 5.0%, non-inferiority p = 0.107) in the ITT analysis, and -4.6% (95% CI: -12.5% to 3.0%, non-inferiority p = 0.179) in the PP analysis.
Conclusions: Optimized empirical therapy demonstrated comparable efficacy to susceptibility testing guided therapy for treating refractory H. pylori infection.
PP-03-331
Enteric Glial Induce Tr17 Cells to Exert Immunosuppressive Effects in DSS Colitis
Jiahui Yang and Shuixiang He
Department of Gastroenterology, The First Affiliated Hospital Of Xi'an Jiaotong University, Xi'an, China
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Background: RORγt+ Foxp3+ regulatory T (Treg) cells, known as Tr17 cells, are a novel subset of Tregs that modulate immune responses. The role of Tr17 cells in the pathogenesis of inflammatory bowel disease (IBD) is not well understood. Our previous studies demonstrated that enteric glial cells (EGCs), which may serve as potential antigen-presenting cells, can exert immunosuppressive effects by inducing Tregs. We further investigated the how EGCs affect Tr17 cells.
Materials and Methods: Using C57 and EGC-ablated (GFAP-HSV-TK) mice, we induced DSS colitis. Some mice received fluorocitrate to inhibit glial metabolism. Immunofluorescence assessed the spatial relationship between cells. Flow cytometry (FACS) evaluated the proportions of Tr17, Foxp3+ Treg and CD39+ CD73+ T cells in mesenteric lymph nodes and colonic lamina propria. Cell co-culture assays evaluated Tr17 cell induction, and mixed lymphocyte reactions assessed their suppressive function. RNA-seq and PCR were conducted on EGCs post-co-culture to identify relevant molecular pathways.
Results: EGCs were found in direct contact with Tr17 cells, which were significantly reduced upon EGC ablation, correlating with increased colitis severity. FC treatment did not notably affect Tr17 cell proportions or inflammation. In vitro, EGCs induced Tr17 cell through contact-dependent mechanisms, with Tr17 cells showing superior suppression of T cell activation compared to conventional Tregs. High expression of CD39 and CD73 was noted in Tr17 cells. Key molecules like I-CAM1, V-CAM1, and CCL2 and CCL7 may mediate these interactions.
Conclusion: EGCs mitigate DSS colitis through contact-dependent induction of Tr17 cells, suggesting a novel pathway for immune regulation in IBD.
PP-03-332
Can impotence caused by Adalimumab be reversed? and Crohn’s be managed with alternatives like Ustekinumab
Asif Yasin, Ali Qamar, Zeshan Pervaiz and Athesham Zafar
Walsall Manor Hospital Nhs Trust Uk, Walsall, United Kingdom
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Tumor Necrosis Factor-alpha (TNF-α) inhibitors, such as adalimumab, are widely used in treating autoimmune conditions. We present a case of erectile dysfunction (ED) induced by adalimumab, which resolved upon switching to Ustekinumab, another biologic drug.
Case Presentation: A 50-year-old male with Crohn’s disease (CD) was treated with adalimumab. He developed progressive ED following the initiation of adalimumab. The Naranjo causality assessment score indicated a probable association between the TNF-α inhibitor and the adverse event.
Intervention: Adalimumab was discontinued, and the patient was transitioned to Ustekinumab for continued disease management.
Outcome: The patient's ED resolved after switching to Ustekinumab. Follow-up assessments confirmed sustained improvement in erectile function.
Conclusion: This case suggests a potential link between TNF-α inhibitors and ED, highlighting the importance of considering such adverse events in clinical practice. The resolution of ED upon switching to Ustekinumab underscores the potential role of interleukin pathways in maintaining sexual function and suggests that ED may be specific to TNF-α inhibitors. Clinicians should remain vigilant for ED in patients undergoing TNF-α inhibitor therapy and consider alternative biologic agents when appropriate.
This case emphasizes the dynamic nature of therapeutic interventions in managing Crohn's Disease, highlighting the need for personalized treatment approaches. Further investigation into the mechanisms underlying these associations and the long-term safety profiles of different biologic agents is essential for optimizing patient care and minimizing treatment-related adverse events.
PP-03-333
Infliximab-induced seronegative Hepatitis Leading to Acute Liver Failure and liver transplant: A Case Report
Asif Yasin, Zeshan Pervaiz, Athesham Zafar and Ali Qamar
Walsall Manor Hospital Nhs Trust Uk, Walsall, United Kingdom
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Infliximab, a TNF-α inhibitor used for autoimmune disorders like ulcerative colitis (UC), can occasionally cause drug-induced liver injury (DILI) and seronegative hepatitis. Unlike typical autoimmune hepatitis (AIH), this condition often lacks autoimmune antibodies. While most cases resolve positively, severe instances may lead to acute liver failure and require transplantation.
A 50-year-old woman with UC presented with abnormal liver function tests. She had no abdominal symptoms and her appetite and weight were stable. She had recently traveled to Tenerife and was treated with two doses of infliximab, being intolerant to thiopurines. There was no history of excessive alcohol consumption. Her liver function deteriorated rapidly, with bilirubin levels exceeding 100 and elevated ALT and ALP, despite a normal liver screen. A liver biopsy revealed seronegative hepatitis, leading to an orthotopic liver transplant within four weeks. Post-transplant, she is well on immunosuppressive medications.
This case highlights the complexity of managing IBD and potential infliximab-induced liver complications. Recognizing infliximab-induced hepatitis without autoantibodies and balancing effective IBD treatment with liver injury monitoring are significant challenges. A speculative model suggests that an unknown drug metabolite may become antigenic, causing hepatitis. Early recognition and diagnosis of drug-induced liver injury are crucial, emphasizing vigilant liver function monitoring in patients on immunomodulatory therapies and the development of diagnostic pathways to prevent severe complications.
Healthcare providers should monitor hepatic complications closely in patients on infliximab, promptly investigate abnormal liver tests, and consider early referral for specialized management if DILI is suspected.
PP-03-334
Hemorrhoid Ligation as an Effective Treatment Option for Hemorrhoids: An Evidence-Based Case Report
Ahmad Nur Aulia1,2, Camelia Camelia1, Muhammad Firhat Idrus1 and Raymond Sebastian Purwanta2
1Pusat Endoscopy Saluran Cerna - RSUPN Dr. Cipto Mangunkusumo, Jakarta Pusat, Indonesia;2Bali International Hospital, IHC Pertamedika, Sanur, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Hemorrhoids are swollen veins of the hemorrhoidal plexus characterized by symptoms resulting from prolapse and dilatation. The disease often presents with inflammation, pruritus, hemorrhage, and thrombosis. Several risk factors contribute to the development of hemorrhoids, including chronic constipation, low fiber diet, excessive straining, pregnancy, heredity, age, and occupation. The treatment varies with the prolapse grade, with grades I and II treated clinically. Grades III and IV, or those unresponsive to clinical therapy, often require more extensive treatments, ranging from non-operative and minimally invasive procedures to surgical techniques.
This study demonstrated a case of an 84-year-old woman presented with recurrent bloody stool with a history of osteoporosis, hypertension, and routine consumption of NSAID. The patient underwent a colonoscopy and received band-ligation treatment for internal hemorrhoids. This case underscores challenges in the treatment strategies of hemorrhoids. Endoscopic rubber band ligation merges traditional elastic ligation with endoscopic examination, allowing for improved control, photographic documentation, and sedation within a hospital setting. Endoscopic band ligation has recently been reported as an efficient method for treating symptomatic hemorrhoids grades II and III due to its low complication rates and high safety.
Ileocolitis as a manifestation of Henoch-Schonlein Purpura: A case report
Yaditta Mirdania1, Mario Steffanus1 and Muhammad Firhat Idrus2
1Fellow of gastroenterohepatology Departement of Internal medicine Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia;2Division of Gastroenterology Departement of Internal medicine Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta Pusat, Indonesia
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Introduction: Henoch-Schonlein purpura is a form of immunoglobulin A vasculitis that is relatively rare in adults. This disease may have cutaneous and systemic manifestations, such as gastrointestinal, renal, and musculoskeletal involvements.
Case Description: A woman, aged 64 years, complained of increasing reddish spots on both legs since 1 month prior to admission. The spots initially appear as red spots, then within 12 hours they widen and increase in number, without itching or pain. The patient also complained of bloody stool since 1 week prior to admission. On physical examination, the patient was afebrile, and vital signs were normal, the lower limbs were found multiple hyperpigmented macules with no tenderness. The Skin Biopsy Results was consistent with vasculitis, with visible damaged blood vessels surrounded by inflammatory cells, neutrophils and nuclear dust.
Colonoscopy Results was Ileocolitis caused HSP manifestation. Colonic Histopathology: Histology is consistent with low grade tubular adenoma, colonic dysplasia in active chronic ileocolitis with mild distortion. In this patient, a diagnosis of HSP was made with ileocolitis caused by HSP, and steroid therapy was given. Haematoschezia was reported to improve along with improvement of the skin lesions.
Discussion: Gastrointestinal manifestations can be found in 44.7% of HSP sufferers, including abdominal pain, diarrhea and GI bleeding. Skin lesions usually be seen as the first manifestation of HSP, but in 10-15% of cases, gastrointestinal manifestation precede the skin lesions. Management of GI manifestations in HSP is oral steroids and supportive therapy.
PP-03-341
Manometric evaluation of Functional Constipation and Response to Biofeedback therapy: A tertiary Care Experience
Sujit Chaudhuri and Agnibha Dutta
Manipal Hospital, Broadway, Salt, Lake, Kolkata, India
Poster (Day 3), November 24, 2024, 8:30 AM - 5:00 PM
Objectives: In patients with refractory chronic constipation where organic causes have been ruled out, colonic transit study and anorectal manometry with balloon expulsion tests are important diagnostic tool to assess and categorise the disease. In patients with dyssynergic defecation (DSD), biofeedback therapy is treatment of choice. This study assess the prevalence of DSD in patients of Chronic idiopathic constipation and response of Biofeedback therapy in patients with DSD.
Materials and Methods: Between January 2020 and May 2024, consecutive patients attending Gastroenterology OPD at Manipal Hospitals, Broadway has been evaluated as per standard protocol. Patients suspected to have functional defecatory disorders underwent anorectal manometry with balloon expulsion test. Patients willing to undergo biofeedback therapy received the same and treatment response was assessed.
Results: Total 310 patients underwent anorectal manometry for chronic constipation. Mean age was 43±19 years. Of them 141 patients found to have DSD. Type I (129, 91,4%) was commonest. Twenty eight patients underwent biofeedback therapy with median 4 sessions (IQR=1). Type I DSD was in the majority (N=27). Twenty six patients had satisfactory response.
Conclusion: DSD is a major cause of refractory constipation. Sessions of biofeedback therapy offers good outcome in this subgroup of patients.
PP-03-346
A Post-hoc Analysis : Association Between Metabolic Dysfunction-Associated Fatty Liver Disease Subgroups and Long-term Outcomes
1Gastroenterology and Hepatology Unit, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;2Department of Pathology, Hospital Sultanah Bahiyah, Alor Setar, Malaysia
Poster (Day 2), Poster Area, November 23, 2024, 8:30 AM - 5:00 PM
Objectives: Metabolic dysfunction-associated fatty liver disease (MAFLD) encompasses a diverse patient group. This study aims to assess liver disease severity and long-term clinical outcomes in adult MAFLD patients by subgroup classification.
Materials and methods: This single-centre prospective study included MAFLD patients who underwent baseline liver biopsy. Analysis was conducted for three subgroups: MAFLD with type 2 diabetes (T2D) (group 1), MAFLD without T2D but were overweight or obese (group 2), and lean MAFLD without T2D who had at least two metabolic risk abnormalities (group 3).
Results: Among 202 patients, 67.8%, 30.7%, and 1.5% were in groups 1, 2, and 3, respectively. The data for patients in group 1 [mean age 56.5±10.0 years old; male, 47.4%] and group 2 [mean age 47.4±10.8 years old; male, 48.4%] were analysed. The median follow-up was 7 (4–8) years. Group 1 patients were more likely to have metabolic dysfunction associated with steatohepatitis (MASH) (p <0.05), fibrotic MASH (p <0.05) and advanced liver fibrosis (p <0.001) compared to group 2 patients. Cumulative incidence of liver-related events, cardiovascular events, malignancy, and mortality of patients in group 1 were 0.69, 2.22, 0.83 and 0.69 per 100 person-years of follow-up, respectively, while in group 2, were 0, 1.43, 0.24 and 0.48 per 100 person-years of follow-up, respectively. The cumulative incidences were not significantly different between patients in group 1 and 2.
Conclusion: T2D is associated with more severe liver disease in MAFLD patients, emphasizing the need for targeted screening and individualized management based on underlying metabolic abnormalities.
PP-03-348
The efficacy and safety of antidepressants and psychotherapy in irritable bowel syndrome: a meta-analysis
Zilin Ma and Guan Zhou Zhou and Xiaoyan Chi and Fei Pan
PLA General Hospital, Beijing, China
Objective: To assess the efficacy and safety of antidepressants and psychotherapy for irritable bowel syndrome (IBS) patients.
Materials and Methods: We conducted a literature search in Medline, Embase, Cochrane Library, and PubMed for randomized controlled trials (RCTs) up to June 2024, investigating the impact of antidepressants and psychotherapy on IBS versus placebo or control. A network meta-analysis with a multivariate consistency model random-effect metaregression was utilized. The primary outcome was the comparative effect of antidepressants or psychotherapy on overall IBS symptoms. Secondary outcomes included efficacy assessment based on antidepressant type or psychological therapy and adverse events.
Results: This study included 74 RCTs (10,128 participants, 69% female): 32 studies compared antidepressants to placebo, and 42 compared psychological therapies to control, encompassing 22 different antidepressants and 36 psychological therapy modalities (Figure 1). Antidepressants generally showed greater efficacy than placebo (relative risk RR 0.84, 95% confidence interval CI 0.78-0.95). Tricyclic antidepressants (TCAs) were notably more effective in reducing overall IBS symptoms (RR 0.66, 95% CI 0.52-0.79), albeit with moderate heterogeneity (I2=49%, P=0.08). Adverse events were more common with antidepressants, but none were serious. Among psychological therapies, cognitive behavior therapy (CBT) was the most efficacious for IBS (RR 0.81, 95% CI 0.68-0.95). No significant differences were observed in adverse effect rates among the various psychotherapies and control therapy.
Conclusion: Antidepressants have proven effective in alleviating IBS symptoms, with TCAs demonstrating the highest efficacy. Concurrently, sychotherapy, particularly CBT, emerges as a promising therapeutic approach. However, the evidence base is limited, potentially exaggerating reatment effectiveness.
PP-03-349
FMT is less effective in UC patients with depression, anxiety, insomnia or poor psychological resilience
Guanzhou Zhou1,2, Xiaolei Liu3, Ronghuan Jiang3, Hong Guo3, Yunsheng Yang2,4 and Lihua Peng2
1School of Medicine, Nankai University, Tianjin;2Clinical Division of Microbiota, Department of Gastroenterology and Hepatology, Microbiota Laboratory;3Department of Medical Psychology, The First Medical Center of Chinese PLA General Hospital;4National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China
Objectives: Fecal microbiota transplantation (FMT) exhibited potential for remission induction in patients with ulcerative colitis (UC) while with uncertain efficacy. Those patients also suffered from abnormal psychological condition like depression and anxiety, which affected FMT efficacy. Therefore, we conducted a series of questionnaires about mental health in patients receiving FMT for UC to predict its effectiveness.
Materials and Methods: Patients with active UC were enrolled in this study. Before the first FMT, four questionnaires were conducted and their cut-off values were based on their individual criterion. It included Patient Health Questionnaire depression scale (PHQ-9), Generalized Anxiety Disorder Scale (GAD-7), Insomnia Severity Index (ISI-7) and Connor-Davidson Resilience Scale (CD-RISC-25). The abnormal scores were defined as ≥5, ≥4, >7 and <70 individually.
Results: All sixty-one patients with active UC received at least once FMT therapy. The mean Mayo score decrease from 7.95±2.15 to 5.18±2.69 (P<0.001). Patients with abnormal PHQ-9, ISI-7 and CD-RISC-25 scores decreased less in the Mayo assessment than patients with normal scores (1.885 ±2.142 vs. 3.457±2.571, P=0.014; 1.467±2.066 vs. 3.371±2.636, P=0.017; 3.600±3.043 vs. 1.550±2.188, P=0.027) (Figure1).
Additionally, we performed a logistic regression analysis to identify predictors of FMT efficacy. The abnormal scores on PHQ-9, GAD-7, ISI-7 and CD-RISC-25 indicated a poor response to FMT therapy. The regression model showed a 76.19% of specificity and 64.29% of sensitivity with 0.806 of AUC.
Conclusion: Patients with abnormal PHQ-9, GAD-7, ISI-7 and CD-RISC-25 scores benefited less from the FMT treatment and those abnormal scores were promising predictors of poor response to FMT
PP-03-351
PROFILE OF COLORECTAL CANCER AT HAJI GENERAL HOSPITAL MEDAN IN 2019-2023
Dewi Murni Sartika1,2, Imelda Rey2, Taufik Sungkar2, Masrul Lubis2 and Ilhamd Ilhamd2
1RSU Haji Medan, Medan, Indonesia;2Haji Adam Malik General Hospital, Medan, Indonesia
Poster (Day 3), Poster Area, November 24, 2024, 8:30 AM - 5:00 PM
Background: Colorectal cancer is the third most common cancer in the world and the second leading cause of cancer – related death. According to Globocan in 2012, the incidence of colorectal cancer in Indonesia was 12.8 per 100.000 adults with a mortality of 9.5% of all cancer cases.
The prevalence of colorectal cancer continuous to increase. This study aims to determine the characteristics of colorectal cancer patients at Haji General Hospital Medan as one of the referral hospital in North Sumatera Province.
Methode: This retrospective study to the medical record of colorectal cancer patiens hospitalized at Haji General Hospital Medan in 2019-2023. The uncomplete data will be excluded from this study. The collected characteristics data was demographic, location of tumor, degree of differentiation. Univariate analysis was carried out using statistic program.
Result: Total 102 colorectal cancer patients hospitalized in the last 5 years. Most patients were males (61,8%), average age 54 years old (33-78 years old). Most cancers located in the rectum (64,7%), left sided (20,6%), and right sided (14,7%). The degree of differentiation were obtained well-differentiated (58,8%), moderate-differentiated 23,5%, and poorly differentiated (17,7%)
Conclusion: Colorectal cancer was most common among males (61,8%), located in the rectum (64,7%), and 58,8% well differentiated.
期刊介绍:
Journal of Gastroenterology and Hepatology is produced 12 times per year and publishes peer-reviewed original papers, reviews and editorials concerned with clinical practice and research in the fields of hepatology, gastroenterology and endoscopy. Papers cover the medical, radiological, pathological, biochemical, physiological and historical aspects of the subject areas. All submitted papers are reviewed by at least two referees expert in the field of the submitted paper.