Prevalence and antibiotic resistance profile of UTI-causing uropathogenic bacteria in diabetics and non-diabetics at the Maternity and Children Hospital in Jeddah, Saudi Arabia.

IF 4 2区 生物学 Q2 MICROBIOLOGY
Frontiers in Microbiology Pub Date : 2024-11-28 eCollection Date: 2024-01-01 DOI:10.3389/fmicb.2024.1507505
Peter F Farag, Hamzah O Albulushi, Mohammed H Eskembaji, Mohammad F Habash, Mohammed S Malki, Muayad S Albadrani, Ahmed M Hanafy
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引用次数: 0

Abstract

Introduction: One of the most prevalent and recurrent infectious diseases that can range from moderate to fatal is urinary tract infection (UTI). Broad-spectrum antibiotics are the only management strategy for UTIs in ambulators and hospital stays. Due to the ongoing emergence of antibiotic resistance among uropathogens, there is a need for proper selection of antibiotics for empirical therapy against UTIs. This study aimed to compare the etiological profiles and antibiotic susceptibility patterns between diabetic and non-diabetic UTI female patients from the Maternity and Children Hospital in Jeddah, Saudi Arabia.

Methods: Urine samples from different age categories of female UTI patients were collected from January 2021 to June 2023. The positive urine cultures with a single pathogen were selected and all bacterial isolates were identified by MALDI-TOF/MS system. Antibiotic susceptibility testing was done using VITEK-2. Our study included 2,245 female patients, of which 1825 (81%) were non-diabetic and 420 (19%) were diabetic.

Results: The results showed a significant relationship (p = 0.00063) between the average age and the number of diabetic UTI patients. Gram-negative bacilli were more dominant (84.7%, n = 1903) than gram-positive cocci (15.3%, n = 342). Escherichia coli (40.8%) was the most prevalent pathogen identified with a significant (p < 0.0001) increase in non-diabetic (45.26%) than diabetic UTI patients (21.43%). Proteus mirabilis (10.2%) and Pseudomonas aeruginosa (8.7%) followed E. coli in pathogen distribution. Among gram-positive species, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus saprophyticus were found in all age groups of diabetic and non-diabetic UTI patients. The findings showed that the most resistant bacteria from patients with non-diabetic UTIs were found to be resistant to amoxicillin (37.7%) and ampicillin (40%), while the most resistant bacteria from patients with diabetes were found to be resistant to tetracycline (43.3%) and cephalothin (43.5%). In patients with UTIs, ciprofloxacin was found to be the most effective antibiotic against all bacterial species.

Discussion: According to the results, we concluded that the UTI etiological profiles varied among different ages. Ciprofloxacin is a safe medication with optimal sensitivity that can be used to treat both gram-positive and gram-negative bacteria.

沙特阿拉伯吉达妇幼医院糖尿病患者和非糖尿病患者尿路感染致病细菌的患病率和抗生素耐药性分析
导读:尿路感染(UTI)是最普遍和复发的传染病之一,可以从中度到致命。广谱抗生素是门诊和住院期间尿路感染的唯一管理策略。由于尿路病原体中抗生素耐药性的持续出现,需要正确选择抗生素进行针对尿路感染的经验性治疗。本研究旨在比较沙特阿拉伯吉达妇幼医院糖尿病和非糖尿病女性尿路感染患者的病因学特征和抗生素敏感性模式。方法:收集2021年1月至2023年6月不同年龄段女性尿路感染患者的尿液样本。采用MALDI-TOF/MS系统对尿培养阳性病原菌进行鉴定。采用VITEK-2进行药敏试验。本研究纳入2245例女性患者,其中1825例(81%)为非糖尿病患者,420例(19%)为糖尿病患者。结果:平均年龄与糖尿病尿路感染人数之间存在显著相关(p = 0.00063)。革兰氏阴性杆菌(84.7%,n = 1903)高于革兰氏阳性球菌(15.3%,n = 342)。病原菌最多的是大肠杆菌(40.8%),其次是奇异变形杆菌(10.2%)和铜绿假单胞菌(8.7%)。革兰氏阳性菌中,粪肠球菌、金黄色葡萄球菌和腐生葡萄球菌在糖尿病和非糖尿病尿路感染患者各年龄组均有发现。结果显示,非糖尿病尿路感染患者中耐药最多的细菌是阿莫西林(37.7%)和氨苄西林(40%),而糖尿病患者中耐药最多的细菌是四环素(43.3%)和头孢菌素(43.5%)。在尿路感染患者中,环丙沙星被发现是对所有细菌种类最有效的抗生素。讨论:根据结果,我们得出结论,尿路感染的病因在不同年龄之间存在差异。环丙沙星是一种安全的药物,具有最佳的敏感性,可用于治疗革兰氏阳性和革兰氏阴性细菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.70
自引率
9.60%
发文量
4837
审稿时长
14 weeks
期刊介绍: Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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