Green synthesis of silver nanoparticles using Amomum nilgiricum leaf extracts: preparation, physicochemical characterization and ameliorative effect against human cancer cell lines.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI:10.1007/s10616-024-00674-7

Narasimhamurthy Konappa, Rajeshwari H Patil, Anupama S Kariyappa, Soumya Krishnamurthy, Niranjana Siddapura Ramachandrappa, Rahul Krishnappa, Srinivas Chowdappa

{"title":"Green synthesis of silver nanoparticles using Amomum nilgiricum leaf extracts: preparation, physicochemical characterization and ameliorative effect against human cancer cell lines.","authors":"Narasimhamurthy Konappa, Rajeshwari H Patil, Anupama S Kariyappa, Soumya Krishnamurthy, Niranjana Siddapura Ramachandrappa, Rahul Krishnappa, Srinivas Chowdappa","doi":"10.1007/s10616-024-00674-7","DOIUrl":null,"url":null,"abstract":"The present study to production of silver nanoparticles (AgNPs) by leaf extracts of A. nilgiricum and to evaluate the activity of anticancer by using AgNPs against cancer cell lines such as MCF-7, HEPG2, H9C2, HEK293 and H1975. The synthesized AgNPs were characterized by using UV-Vis spectroscopy, EDS, FT-IR, XRD, DLS, SEM and HRTEM with SAED patterns. The surface plasmon resonance (SPR) of AgNPs formed a peak centered at 427 nm by UV-Vis analysis. FTIR analysis reveals that existence of functional groups subjected to silver ions reduction to metallic silver. Crystalline form of the AgNPs was assessed by XRD analysis, four spectral peaks at 111, 200, 220, and 311 were formed and zeta potential peak was found at 28.5 mV indicating the higher stability. The size average diameter of the AgNPs was between 27 and 30 nm by TEM and SEM analysis was reveals the morphology of AgNPs as elongated, irregular and aggregated and some particles are spherical. EDX analysis confirmed the elemental composition of AgNPs with 81.43% Ag. The average diameter of AgNPs was found 21.49 nm in diameter and width was about 12.01 nm by DLS analysis. Cytotoxicity of AgNPs was investigated by using MTT, SRB assay and comet assay was performed as a genotoxicity. The results revealed that AgNPs decreased the viability of cancer cells in a concentration dependent pattern (50 to 350 µg/ml). The influence of AgNPs on cell cycle stop was studied on H1975, HEP-G2 and MCF-7 cells and found that AgNPs could induce sub G0 cell cycle arrest. The AgNPs was also induced DNA fragmentation confirms the DNA damage in nanoparticles treated cell lines. The anticancer action of nanoparticles was analyzed using proapoptotic and antiapoptotic caspase 8 and caspase 3 mRNA expression levels. Finally the results suggested that AgNPs is an effective anticancer agent which induces apoptosis in H1975, HEP-G2 and MCF-7 cells. Based on our studies, further identification of the major compounds of leaf extracts is acceptable.Supplementary information: The online version contains supplementary material available at 10.1007/s10616-024-00674-7.","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 1","pages":"16"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631834/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10616-024-00674-7","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The present study to production of silver nanoparticles (AgNPs) by leaf extracts of A. nilgiricum and to evaluate the activity of anticancer by using AgNPs against cancer cell lines such as MCF-7, HEPG2, H9C2, HEK293 and H1975. The synthesized AgNPs were characterized by using UV-Vis spectroscopy, EDS, FT-IR, XRD, DLS, SEM and HRTEM with SAED patterns. The surface plasmon resonance (SPR) of AgNPs formed a peak centered at 427 nm by UV-Vis analysis. FTIR analysis reveals that existence of functional groups subjected to silver ions reduction to metallic silver. Crystalline form of the AgNPs was assessed by XRD analysis, four spectral peaks at 111, 200, 220, and 311 were formed and zeta potential peak was found at 28.5 mV indicating the higher stability. The size average diameter of the AgNPs was between 27 and 30 nm by TEM and SEM analysis was reveals the morphology of AgNPs as elongated, irregular and aggregated and some particles are spherical. EDX analysis confirmed the elemental composition of AgNPs with 81.43% Ag. The average diameter of AgNPs was found 21.49 nm in diameter and width was about 12.01 nm by DLS analysis. Cytotoxicity of AgNPs was investigated by using MTT, SRB assay and comet assay was performed as a genotoxicity. The results revealed that AgNPs decreased the viability of cancer cells in a concentration dependent pattern (50 to 350 µg/ml). The influence of AgNPs on cell cycle stop was studied on H1975, HEP-G2 and MCF-7 cells and found that AgNPs could induce sub G0 cell cycle arrest. The AgNPs was also induced DNA fragmentation confirms the DNA damage in nanoparticles treated cell lines. The anticancer action of nanoparticles was analyzed using proapoptotic and antiapoptotic caspase 8 and caspase 3 mRNA expression levels. Finally the results suggested that AgNPs is an effective anticancer agent which induces apoptosis in H1975, HEP-G2 and MCF-7 cells. Based on our studies, further identification of the major compounds of leaf extracts is acceptable.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-024-00674-7.

查看原文本刊更多论文

利用砂砂叶提取物绿色合成纳米银：制备、理化性质及对人类癌细胞的改善作用。

本研究探讨了用银纳米粒子（AgNPs）制备银纳米粒子，并利用AgNPs对MCF-7、HEPG2、H9C2、HEK293和H1975等肿瘤细胞株的抗癌活性。利用UV-Vis光谱、EDS、FT-IR、XRD、DLS、SEM和HRTEM对合成的AgNPs进行了表征。AgNPs的表面等离子体共振（SPR）在427 nm处形成一个中心峰。FTIR分析表明，银离子还原成金属银后存在官能团。通过XRD分析发现，AgNPs在111、200、220和311处形成4个谱峰，在28.5 mV处发现zeta电位峰，表明AgNPs具有较高的稳定性。TEM和SEM分析表明，AgNPs的平均粒径在27 ~ 30 nm之间，形貌呈细长状、不规则状和聚集状，部分颗粒呈球形。EDX分析证实AgNPs的元素组成为81.43% Ag。DLS分析发现AgNPs的平均直径为21.49 nm，宽度约为12.01 nm。采用MTT法、SRB法和comet法研究AgNPs的细胞毒性。结果显示，AgNPs以浓度依赖性模式降低癌细胞的活力（50 ~ 350µg/ml）。在H1975、HEP-G2和MCF-7细胞上研究了AgNPs对细胞周期阻滞的影响，发现AgNPs可以诱导亚G0细胞周期阻滞。AgNPs还诱导DNA断裂，证实了纳米颗粒处理细胞系的DNA损伤。采用促凋亡和抗凋亡caspase 8和caspase 3 mRNA表达水平分析纳米颗粒的抗癌作用。结果表明，AgNPs是一种有效的抗癌药物，可诱导H1975、HEP-G2和MCF-7细胞凋亡。根据我们的研究，进一步鉴定叶提取物的主要化合物是可以接受的。补充信息：在线版本包含补充资料，可在10.1007/s10616-024-00674-7获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.