Elucidating the role of the genus Pseudomonas involved in coumarin degradation.

Abstract

Coumarin, a synthetic chemical and phytotoxin, exhibits hepatotoxicity and carcinogenicity, posing threats to both human health and environmental safety. Microbial degradation effectively mitigates environmental contamination. In this study, a coumarin-degrading bacterial consortium designated as XDS-7 with Pseudomonas as the key degrader was obtained. However, there is a lack of comprehensive perspective on the key role of the genus Pseudomonas involved in coumarin degradation. We employed the consortium XDS-7 as a model system to investigate the critical role of the genus Pseudomonas involved in coumarin degradation. Metagenomic binning analysis indicated that bin 14 (Pseudomonas sp.) contains the full complement of genes required for coumarin degradation. A coumarin-degrading bacterium, Pseudomonas sp. strain X4, was isolated from consortium XDS-7 using a traditional enrichment method supplemented with chloramphenicol. Genomic analysis demonstrated that strain X4 carries a suite of genes to completely degrade coumarin. Bioinformatics analysis revealed that putative coumarin-degrading bacteria are widely distributed across diverse bacteria of the genus Pseudomonas. In addition, strain X4 completely removed 100 mg kg^-1 of coumarin from contaminated soil within 48 h and 100 mg L^-1 of coumarin from contaminated wastewater within 4 h. This study will greatly enhance our understanding and utilization of these valuable bioresources.