{"title":"Reusable EWOD-based microfluidic system for active droplet generation.","authors":"Suhee Park, Jaewook Ryu, Ki-Ho Han","doi":"10.1039/d4lc00744a","DOIUrl":null,"url":null,"abstract":"<p><p>Droplets are essential in a wide range of microfluidic applications, but traditional passive droplet generation methods suffer from slow response speed and the need for precise flow rate adjustment. Here, we present an active droplet generation method through electrowetting-on-dielectric (EWOD). Electrowetting is a technique that uses an electric field to change the wettability of a surface. In our method, we apply an electric field to the laminar flow of the dispersed and continuous phases in a microchannel, which induces the discretization of the dispersed thread and leads to droplet formation. A key feature of the proposed active droplet-generating microfluidic device is the reusability of the EWOD actuation substrate, dramatically reducing operational costs. In addition, this approach offers significant advantages over passive methods, including fast response speeds, a wider range of droplet sizes, and greater control over droplet size. In addition, the ultrathin polymer film used in this device allows for a low electrowetting voltage, which helps to prevent damage to encapsulated cells. We believe that our active droplet generation method is a promising new method for generating droplets in microfluidic applications. It is faster, more versatile, and more precise than passive methods, making it ideal for a wide range of applications, including single-cell genomics and drug discovery.</p>","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":" ","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d4lc00744a","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Droplets are essential in a wide range of microfluidic applications, but traditional passive droplet generation methods suffer from slow response speed and the need for precise flow rate adjustment. Here, we present an active droplet generation method through electrowetting-on-dielectric (EWOD). Electrowetting is a technique that uses an electric field to change the wettability of a surface. In our method, we apply an electric field to the laminar flow of the dispersed and continuous phases in a microchannel, which induces the discretization of the dispersed thread and leads to droplet formation. A key feature of the proposed active droplet-generating microfluidic device is the reusability of the EWOD actuation substrate, dramatically reducing operational costs. In addition, this approach offers significant advantages over passive methods, including fast response speeds, a wider range of droplet sizes, and greater control over droplet size. In addition, the ultrathin polymer film used in this device allows for a low electrowetting voltage, which helps to prevent damage to encapsulated cells. We believe that our active droplet generation method is a promising new method for generating droplets in microfluidic applications. It is faster, more versatile, and more precise than passive methods, making it ideal for a wide range of applications, including single-cell genomics and drug discovery.
期刊介绍:
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.