A novel ratiometric electrochemical aptasensor based on graphene quantum dots/Cu-MOF nanocomposite for the on-site determination of Staphylococcus aureus

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2024-12-13 DOI:10.1016/j.jhazmat.2024.136845

Xiaofeng Lin, Chenghao Liu, Qiaowen Lei, Xinru Nan, Yunxiao Zhu, Jing Liao, Zhizhi Du, Chenyi Ye, Yixian Xiong, Min Yang, Xiaojun Fang, Yan Luo, Qitong Huang

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Abstract

The sensitive detection of Staphylococcus aureus (S. aureus) holds great practical importance for ensuring public health and food safety. In this study, a sensitivity and stability ratiometric electrochemical aptasensor using graphene quantum dots/[Cu_2.5 (benzotriazole-5-COO)_1.5 (benzotriazole-5-COOH)_0.5 (μ-Cl)_0.5 (μ₃-OH)-(H₂O)]·3H₂O nanocomposite (GQDs/Cu-MOF) was constructed for S. aureus detection. The GQDs enhanced the sensitivity of the electrochemical aptasensor due to their excellent conductivity and provided stability through their abundant carboxyl groups. The Cu-MOF, possessing electrical activity, not only enhanced the performance of the electrochemical aptasensor but also served as a signal label. The single-stranded DNA1 (S1) was immobilized on the surface of a GQDs/Cu-MOF/screen-printed carbon electrode (S1/GQDs/Cu-MOF/SPCE) as the sensing interface. Subsequently, the S1/GQDs/Cu-MOF/SPCE was hybridized with the probe DNA-ferrocene (S2-Fc), resulting in the generation of electrochemical signals from Cu-MOF (I_Cu-MOF) and S2-ferrocene (I_S2-FC) within the system. However, the electron transfer performance of DNA at the sensing interface was compromised, leading to a reduction in the I_Cu-MOF. When S. aureus was present in the system, S2-Fc reacted with it and detached from the sensing surface, resulting in a gradual decrease in I_S2-FC and an increase in I_Cu-MOF. Then a ratiometric electrochemical aptasensor was established for S. aureus detection with remarkable sensitivity (0.97 CFU·mL^-1), excellent stability, and a broad linear range. Furthermore, the aptasensor was successfully applied to detect S. aureus in tap water, milk, Lonicera japonica, urine, and Zhangjiang River. Additionally, this aptasensor design can be adapted for the detection of other foodborne pathogens, which indicates that the design scheme of the aptasensor has good universality.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.