Harnessing light-harvesting chlorophyll a/b-binding proteins for multiple abiotic stress tolerance in Chlamydomonas reinhardtii: Insights from genomic and physiological analysis.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Ali Raza, Yiran Li, Hafiz Muhammad Rizwan, Asadullah Khan, Yuqi Peng, Chunli Guo, Zhangli Hu
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Abstract

Light-harvesting chlorophyll a/b-binding proteins (LHC) of photosystem II perform key functions in various processes, e.g., photosynthesis, development, and abiotic stress responses. Nonetheless, comprehensive genome-wide investigation of LHC family genes (CrLHCs) has not been well-reported in single-cell alga (Chlamydomonas reinhardtii). Here, we discovered 61 putative CrLHC genes in the C. reinhardtii genome and observed that most genes demonstrate stable exon-intron and motif configurations. We predicted five phytohormones- and six abiotic stress-interrelated cis-regulatory elements in promoter regions of CrLHC. Likewise, 19 miRNAs targeting 42 CrLHC genes from 16 unique families were discovered. Besides, we identified 400 transcription factors from 13 families, including ERF, GATA, CPP, bZIP, C3H, MYB, SBP, Dof, bHLH, C2H2, G2-like, etc. Protein-protein interactions and 3D structures provided insight into CrLHC proteins. Gene ontology and KEGG-based enrichment advocated their role in light responses, photosynthesis, and energy metabolisms. Expression analysis highlighted the shared and unique roles of many CrLHC genes against different abiotic stresses (UV-C, green light, heat, nitric oxide, cadmium, nitrogen starvation, and salinity). Under salinity stress, antioxidant enzyme activity, reactive oxygen species markers, photosynthesis-related traits and pigments were significantly affected. Briefly, this comprehensive genomic and physiological study shed light on the impact of CrLHC genes in abiotic stress tolerance and set the path for future genetic engineering experiments.

利用捕光叶绿素a/b结合蛋白促进莱茵衣藻耐多种非生物胁迫:来自基因组和生理分析的见解。
光系统II的光捕获叶绿素a/b结合蛋白(LHC)在光合作用、发育和非生物胁迫响应等多种过程中发挥关键作用。尽管如此,在单细胞藻类(莱茵衣藻)中,对LHC家族基因(crlhc)的全面全基因组研究尚未有很好的报道。在这里,我们发现了61个假定的CrLHC基因,并观察到大多数基因表现出稳定的外显子-内含子和基序结构。我们在CrLHC的启动子区域预测了5种植物激素和6种非生物胁迫相关的顺式调控元件。同样,我们还发现了来自16个独特家族的42个CrLHC基因的19个mirna。此外,我们从13个家族中鉴定出400个转录因子,包括ERF、GATA、CPP、bZIP、C3H、MYB、SBP、Dof、bHLH、C2H2、G2-like等。蛋白质相互作用和3D结构提供了对CrLHC蛋白的深入了解。基因本体和基于kegg的富集主张它们在光响应、光合作用和能量代谢中的作用。表达分析强调了许多CrLHC基因对不同非生物胁迫(UV-C、绿光、热、一氧化氮、镉、氮饥饿和盐度)的共同和独特作用。在盐胁迫下,抗氧化酶活性、活性氧标记物、光合相关性状和色素受到显著影响。总之,这项全面的基因组和生理学研究揭示了CrLHC基因在非生物胁迫耐受中的影响,为未来的基因工程实验奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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