Description of Hyphococcus formosus sp. nov. and Hyphococcus lacteus sp. nov., isolated from coastal sediment, and reclassification of Marinicaulis flavus as Hyphococcus luteus nom. nov. and Marinicaulis aureus as Hyphococcus aureus comb. nov

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-01-01 DOI:10.1016/j.syapm.2024.126575

Yu-Qi Ye , Xin-Yue Zhang , Hong-Nan Gong , Meng-Qi Ye , Zong-Jun Du

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引用次数: 0

Abstract

During a study on sediment bacterial diversity in coastal China, three bacterial strains, DH-69^T, EH-24, and ECK-19^T, were isolated from coastal sediments off Xiaoshi Island, Weihai. These strains were Gram-staining-negative, aerobic, and coccoid to rod-shaped with prosthecae and flagella. Comparison of the 16S rRNA gene showed that they shared the highest identity values with Hyphococcus flavus MCCC 1K03223^T (96.2–97.6 %), followed by Marinicaulis flavus SY-3-19^T (95.2–96.8 %) and Marinicaulis aureus HHTR114^T (95.2–96.2 %). Genome comparisons using average nucleotide identity (ANI) and average amino acid identity (AAI) suggested that the three novel strains and the three related strains belonged to the same genus, with strains DH-69^T, EH-24, and ECK-19^T identified as two distinct novel species. Pan-genome analysis revealed that 995 core genes were shared among 23 Hyphococcus genomes/MAGs. Secondary metabolites analysis identified a biosynthesis gene cluster for microsclerodermin, a potent antifungal peptide, in the novel strains. Moreover, these newly isolated strains were detected in various ecosystems, with a particular prevalence in marine environments, based on analysis of 500,048 amplicon datasets, underscoring their ecological preference. Based on polyphasic characterizations, strains DH-69^T and EH-24 represent a novel species of the genus Hyphococcus, for which the name Hyphococcus formosus sp. nov. is proposed with the type strain DH-69^T (= MCCC 1H00436^T = KCTC 8010^T). Strain ECK-19^T represents another novel Hyphococcus species, for which the name Hyphococcus lacteus sp. nov. is proposed with the type strain ECK-19^T (= MCCC 1H00435^T = KCTC 8009^T). Furthermore, Marinicaulis flavus and Marinicaulis aureus are proposed to be reclassified as Hyphococcus luteus nom. nov. and Hyphococcus aureus comb. nov., respectively, accompanied by an emended description of the genus Hyphococcus.

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描述从海岸沉积物中分离出的台湾双球菌和乳双球菌，并将黄双球菌重新分类为黄双球菌，将金黄色双球菌重新分类为梳状金黄色双球菌。11月。

在对中国沿海沉积物细菌多样性的研究中，从威海小石岛沿海沉积物中分离到3株细菌DH-69T、EH-24和ECK-19T。这些菌株革兰氏染色阴性，需氧，球型到杆状，有前体和鞭毛。16S rRNA基因与黄葡萄球菌MCCC 1K03223T的同源性最高（96.2 ~ 97.6%），其次是黄葡萄球菌SY-3-19T（96.2 ~ 96.8%）和金黄色葡萄球菌HHTR114T（96.2 ~ 96.2%）。利用平均核苷酸同源性（ANI）和平均氨基酸同源性（AAI）进行基因组比较，发现3株新菌株和3株亲缘菌株属于同一属，其中菌株DH-69T、EH-24和ECK-19T被鉴定为两个不同的新种。泛基因组分析显示，23个球菌基因组/ mag共有995个核心基因。次级代谢物分析鉴定了新菌株中微硬皮蛋白（一种有效的抗真菌肽）的生物合成基因簇。此外，基于对500,048个扩增子数据集的分析，这些新分离的菌株在各种生态系统中被检测到，特别是在海洋环境中普遍存在，强调了它们的生态偏好。根据多相特征，菌株DH-69T和EH-24代表了Hyphococcus属的一个新种，并与型菌株DH-69T （= MCCC 1H00436T = KCTC 8010T）一起命名为Hyphococcus formosus sp. nov。菌株ECK-19T代表了另一种新型的Hyphococcus，它与类型菌株ECK-19T （= MCCC 1H00435T = KCTC 8009T）一起被命名为Hyphococcus lacteus sp. nov。此外，建议将黄金黄色葡萄球菌和金黄色葡萄球菌重新分类为黄金黄色葡萄球菌和梳状金黄色葡萄球菌。分别于11月11日，并附有对Hyphococcus属的修订描述。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: