Oral administration of crocin reverses memory loss induced by ethanol and nicotine abstinence in adolescent male rats

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-01-18 DOI:10.1016/j.neulet.2024.138077

Samaneh Kakhki , Ali Abbaszade-Cheragheali , Seyyed Pouria Tafti , Atefeh Shirinzadeh Feizabadi , S. Mohammad Ahmadi-Soleimani , Farimah Beheshti

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Abstract

Purpose

Regarding a wide variety of researches conducted with various therapeutic effect of crocin, the main constituent of saffron, the current study aims to assess the efficacy of crocin to improve learning and memory impairment caused by withdrawal following concurrent usage of ethanol (Eth) and nicotine (Nic) in adolescent male rats.

Methods

In order to test memory fucntion, Morris water maze and passive avoidance methods were applied in male Wistar rats undergone adolescent Nic-Eth withdrawal and the effect of crocin treatment was assessed at both behavioral and biochemical levels. The biochemical parameters included the inflammatory cytokines, indicators of oxidative stress and cholinergic metabolism within the hippocampla tissues. Animals were divided into 7 experimental groups as follows: 1) control (saline + saline), 2) nicotine + ethanol, 3–5) nicotine + ethanol + crocin (three doses), 6) nicotine + ethanol + bupropion + naloxone and 7) saline + crocin.

Results

Results indicated that crocin treatment effectively prevented the Nic-Eth withdrawal induced behavioral manifestations of memory impairment when assessed by Morris water maze and passive avoidance tests. In addition, the biochemical alterations (in inflammatory, oxidative and cholinergic parameters) induced by Nic-Eth withdrawal were also ameliorated in rats treated by crocin. Interestingly, the mentioned ameliorative effect of crocin was found to be dose-dependent in most experiments and almost equipotential to that of bupropion and naloxone co-administration, when administered at high doses.

Conclusion

We would like to suggest the crocin treatment as an alternative medication for the management of Nic − Eth withdrawal, however, further studies are required to assess the unknown side effects and high dose tolerability of the drug in human subjects.

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口服藏红花素可逆转乙醇和尼古丁戒断引起的青春期雄性大鼠记忆丧失。

目的：对藏红花的主要成分藏红花素的各种治疗作用进行了广泛的研究，本研究旨在评估藏红花素对青少年雄性大鼠同时使用乙醇（Eth）和尼古丁（Nic）后戒断引起的学习和记忆障碍的改善效果。方法：采用Morris水迷宫法和被动回避法对青春期戒断Nic-Eth的雄性Wistar大鼠进行记忆功能测试，并从行为和生化两方面评价藏红花素治疗的效果。生化指标包括海马组织内炎症因子、氧化应激指标和胆碱能代谢指标。动物被分成7个实验小组如下:1)控制(盐水 + 盐水),2)尼古丁 + 乙醇,乙醇3 - 5)尼古丁 +  + 藏红花素(三剂),6)尼古丁 + 乙醇 + 安非他酮 + 纳洛酮和7)盐水 + 藏红花素。结果：Morris水迷宫和被动回避实验结果显示，藏红花素可有效预防Nic-Eth戒断所致的记忆障碍行为表现。此外，藏红花素还能改善大鼠Nic-Eth戒断引起的生化改变（炎症、氧化和胆碱能参数）。有趣的是，在大多数实验中，发现藏红花素的上述改善作用是剂量依赖的，并且在高剂量时，与安非他酮和纳洛酮共给药几乎相同。结论：我们建议将藏红花素治疗作为治疗Nic - Eth戒断的一种替代药物，但需要进一步的研究来评估未知的副作用和药物在人类受试者中的高剂量耐受性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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