Effect of dietary fat source on the composition of the cecal microbiome in maturing broiler chicken.

IF 4 2区 生物学 Q2 MICROBIOLOGY
Frontiers in Microbiology Pub Date : 2024-11-27 eCollection Date: 2024-01-01 DOI:10.3389/fmicb.2024.1462757
Vidya V Jadhav, Yewande Fasina, Paul C Omaliko, Jian Han, Scott H Harrison
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引用次数: 0

Abstract

Diet has been found to significantly influence gut microbiota throughout various life stages, and gut microbiota have been increasingly shown to influence host physiology, health, and behavior. This study uses 16S rRNA sequencing to examine the effects of six different fat-supplemented diets (canola oil, coconut oil, fish oil, flaxseed oil, lard, and olive oil) on broiler chicken cecal microbial composition and predicted function in comparison with a common and inexpensive fat source (poultry fat). Groups of broilers were fed each of these diets and then evaluated on day 41 and day 55 of age. For both 41- and 55-day samples, Firmicutes and Bacteroidetes phyla were the dominant bacteria in the ceca accounting for 99% of the microbial community. Across the 41- and 55-day samples, treatment time was associated with a stronger and more significant microbiota shift (p < 0.001) than differences in dietary treatment alone (p = 0.117), but dietary treatment combined with treatment time is a significant factor as well (p = 0.047). Sparse partial least squares discriminant analysis was used to explore the more discriminating taxa for each treatment group. For identified species, butyrate production appears to be affected in a diet-specific manner, with many butyrate-producing species being evident for the fish-based diet at day 41 and a few of these species for the flaxseed-based diet at day 55. Predicted functions, as conducted with PICRUSt2, were significant for comparisons between the control and the flaxseed-based dietary treatment group at day 55, with indications of host health benefit for the flaxseed-based diet. Predicted functions found to be significant were for enzymes and pathways such as propionate CoA ligase, aminobutyraldehyde dehydrogenase, vitamin B12-transporting ATPase, thiamine kinase, acetylneuraminate epimerase, and L-tryptophan biosynthesis. This study provides insight surrounding specific dietary fat-based treatments to be investigated further and highlights the importance of polyunsaturated fat sources in poultry feed that may offer a favorable cecal microbial modulation compared to saturated fat sources.

饲粮脂肪来源对成熟肉鸡盲肠微生物组组成的影响。
饮食已被发现在生命的各个阶段显著影响肠道微生物群,肠道微生物群已越来越多地被证明影响宿主的生理、健康和行为。本研究使用16S rRNA测序技术,研究了6种不同脂肪补充日粮(菜籽油、椰子油、鱼油、亚麻籽油、猪油和橄榄油)对肉鸡盲肠微生物组成的影响,并与一种常见且廉价的脂肪来源(家禽脂肪)进行了比较。各组肉鸡分别饲喂这些饲粮,并在第41天和第55天进行评估。在41天和55天的样本中,盲肠中的优势细菌是厚壁菌门和拟杆菌门,占微生物群落的99%。在41天和55天的样本中,治疗时间与更强、更显著的微生物群变化相关(p p = 0.117),但饮食治疗与治疗时间相结合也是一个显著因素(p = 0.047)。采用稀疏偏最小二乘判别分析,探索各处理组判别性较强的分类群。对于已确定的物种,丁酸盐的产量似乎以特定的饮食方式受到影响,在第41天,许多产丁酸盐的物种在以鱼为基础的饮食中表现明显,而在第55天,少数产丁酸盐的物种在以亚麻籽为基础的饮食中表现明显。在第55天,PICRUSt2预测的功能在对照组和以亚麻籽为基础的饮食处理组之间的比较中是显著的,有迹象表明以亚麻籽为基础的饮食对宿主的健康有益。预测功能发现重要的是酶和途径,如丙酸辅酶a连接酶,氨基丁醛脱氢酶,维生素b12运输atp酶,硫胺素激酶,乙酰神经胺酸酯酶和l -色氨酸生物合成。该研究为进一步研究特定的膳食脂肪处理提供了见解,并强调了家禽饲料中多不饱和脂肪来源的重要性,与饱和脂肪来源相比,多不饱和脂肪来源可能提供有利的盲肠微生物调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.70
自引率
9.60%
发文量
4837
审稿时长
14 weeks
期刊介绍: Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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