A pilot study of the relationship between salivary microbial characteristics and endocrine and immune function in patients with burning mouth syndrome.

IF 3.1 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Qianqian Zhang, Xinghan Li, Zhiyi Wang, Shijiao Shen, Junzhe Wang, Junyang Chen, Xiaoran Shi, Shichen Wang, Jun Cao, Yongqiang Deng, He Meng, Lin Ma
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引用次数: 0

Abstract

Objectives: To explore the potential role of oral bacteria in the pathogenesis of burning mouth syndrome (BMS).

Materials and methods: Fifteen patients with BMS and 15 healthy controls were enrolled in this study. The relative abundance and diversity of salivary bacterial strains were analyzed using metagenomic DNA sequencing. The functionality of non-redundant genes was obtained by comparing the mmseqs2 with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. BMS relating salivary protein markers were detected using enzyme-linked immunosorbent assay (ELISA).

Results: Partial least-squares discriminant analysis (PLS-DA) revealed that the salivary bacterial profiles of the BMS group (mean age: 38.9 ± 2.2 years) differed significantly from those of the control group (mean age: 34.1 ± 2.3 years) in terms of the abundance of dominant phyla, classes, orders, families, genera and species. Functional analysis revealed distinct endocrine and immune functions in the BMS group. The salivary cortisol and α-amylase levels were significantly higher, and salivary interleukn-6 levels were significantly lower in the BMS group than in the control group. Species contribution analysis of endocrine and immune functions revealed that Streptococcus mitis was an important contributor to endocrine and immune functions in the BMS group.

Conclusions: Salivary dysbiosis related to endocrine and immune functions may be a possible cause of BMS.

Clinical relevance: These findings could assist in the diagnosis and therapy of BMS.

Clinical trial number: Not applicable.

灼口综合征患者唾液微生物特征与内分泌和免疫功能关系的初步研究。
目的:探讨口腔细菌在灼口综合征发病机制中的潜在作用。材料与方法:选取15例BMS患者和15例健康对照者。采用宏基因组DNA测序技术分析唾液细菌菌株的相对丰度和多样性。通过将mmseqs2与京都基因与基因组百科全书(KEGG)数据库进行比较,获得非冗余基因的功能。采用酶联免疫吸附试验(ELISA)检测BMS相关唾液蛋白标志物。结果:偏最小二乘法判别分析(PLS-DA)显示,BMS组(平均年龄38.9±2.2岁)与对照组(平均年龄34.1±2.3岁)在优势门、纲、目、科、属和种的丰度方面存在显著差异。功能分析显示BMS组具有明显的内分泌和免疫功能。BMS组唾液皮质醇和α-淀粉酶水平显著高于对照组,唾液白细胞介素-6水平显著低于对照组。内分泌和免疫功能的物种贡献分析显示,炎链球菌是BMS组内分泌和免疫功能的重要贡献者。结论:与内分泌和免疫功能相关的唾液生态失调可能是BMS的原因之一。临床意义:这些发现有助于BMS的诊断和治疗。临床试验号:不适用。
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来源期刊
Clinical Oral Investigations
Clinical Oral Investigations 医学-牙科与口腔外科
CiteScore
6.30
自引率
5.90%
发文量
484
审稿时长
3 months
期刊介绍: The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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