3D Culture System for Human Adrenal Glands That Uses a Sequential Processing Medium to Facilitate Cortical-Medullary Cell Development.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2024-12-01 DOI:10.7754/Clin.Lab.2024.240719

Yajie Guo, Bingqian Guo, Yan Huang, Yu Ye, Hongfei Chen, Liying Zhang, Minxi Li, Yifu Wang, Yufang Lv, Jinling Liao, Yang Chen, Yu Long, Jinghang Jiang, Zhongyuan Chen, Yi Guo, Zengnan Mo, Yonghua Jiang

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引用次数: 0

Abstract

Background: The human adrenal gland is composed of the cortex and the medulla, which contain different function cells. The aim of this study was to build a 3D culture system for human adrenal glands.

Methods: Human fetal adrenal tissues were digested into a cell suspension culture and processed in three-phase 3D cultures.

Results: Apparent spheroids could be seen from the 4th day on. After 21 days of 3D culture, steroid synthesis cells were evident via CYP17A1+ immunohistochemical staining and flow cytometry analysis. Electron microscopy analysis showed that these cells were present in lipid droplets in the cytoplasm. Meanwhile, TH+ cells represented catecholamine-producing cells, and these cells exhibited electron density particle gathering in the cytoplasm. Dehydroepiandrosterone and epinephrine syntheses were further confirmed via enzyme-linked immunosorbent assay.

Conclusions: We established a 3D culture system for human adrenal glands by using a sequential processing medium to facilitate cortical-medullary cell development.

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使用顺序处理介质促进皮质-髓细胞发育的人肾上腺三维培养系统。

背景：人肾上腺由皮质和髓质组成，两者含有不同的功能细胞。本研究的目的是建立人肾上腺三维培养系统。方法：将人胎儿肾上腺组织消化成细胞悬浮培养，并进行三相三维培养。结果：第4天起可见明显的球体。3D培养21天后，通过CYP17A1+免疫组化染色和流式细胞术分析可见类固醇合成细胞。电镜分析显示，这些细胞以脂滴形式存在于细胞质中。同时，TH+细胞代表儿茶酚胺产生细胞，这些细胞在细胞质中表现出电子密度粒子聚集。通过酶联免疫吸附试验进一步证实脱氢表雄酮和肾上腺素的合成。结论：利用序贯处理培养基建立了人肾上腺三维培养体系，促进皮质-髓细胞的发育。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.