Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-12-12 DOI:10.1002/cre2.70039

Marzieh Danaei, Milad Mollaali, Vida Fakharmohialdini, Hamidreza Poureslami, Fatemeh Sadat Sajadi, Elham Farokh Gisour, Fatemeh Jahanimoghadam, Aida Gholampour, Mehrnaz Foroudisefat, Arezoo Mirshekari, Raziyeh Shojaeipour

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引用次数: 0

Abstract

Objectives

Lactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S. mutans and lactobacilli in dental caries using Snyder's test, colony counting, and PCR in the primary teeth of Iranian children with dental caries.

Material and Methods

A cross-sectional study was conducted in Kerman, Iran, from March to Jun 2024. After dental examinations, 120 eligible children aged 5–9 were chosen using simple random sampling and classified into four groups based on their decayed, missing, and filled (primary) teeth (dmft) index: Group 1 (dmft = 0), Group 2 (dmft = 4–6), Group 3 (dmft = 7–9), and Group 4 (dmft = 10–13). The salivary levels of lactobacilli and S. mutans were calculated using colony counting (CFU/ml). Moreover, Snyder's test was applied to evaluate caries activity. PCR was also performed for molecular detection of lactobacilli (16S rRNA gene) and S. mutans (gftB gene). Lastly, the association between bacterial counting, molecular findings, and Snyder's test was estimated through statistical methods using SPSS 27.

Results

Significant differences were found between the age and the PCR results of lactobacilli and S. mutans among all dmft Groups (p < 0.05). Moreover, positive significant correlations were observed between the counts of lactobacilli and S. mutans in dmft Group 1 compared to other dmft Groups (p < 0.05). Furthermore, the counts of these two bacteria differed significantly in Snyder's test (p < 0.05). However, Snyder's test differed significantly from the S. mutans PCR (p < 0.05), but not from the lactobacilli PCR (p > 0.05).

Conclusions

The results of the study could potentially be considered a promising and cost-effective screening program to identify children who are susceptible to dental caries.

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伊朗5 ~ 9岁儿童龋齿中变形链球菌和乳酸菌的分子和微生物检测研究

目的：乳酸菌和变形链球菌被认为是导致龋齿的细菌，但很少有研究同时检查乳酸菌和变形链球菌在儿童龋齿中的定量和定性方面。因此，本研究旨在通过Snyder’s试验、菌落计数和PCR检测伊朗龋齿患儿乳牙中变形链球菌和乳酸菌的存在。材料与方法：于2024年3月至6月在伊朗Kerman进行横断面研究。通过口腔检查，采用简单随机抽样的方法，选取符合条件的120名5-9岁儿童，根据乳牙（dmft）指数分为4组：1组（dmft = 0）、2组（dmft = 4-6）、3组（dmft = 7-9）、4组（dmft = 10-13）。用菌落计数（CFU/ml）计算唾液中乳酸杆菌和变形链球菌的水平。此外，还采用Snyder’s试验评估龋活性。采用PCR方法对乳酸菌（16S rRNA基因）和变形链球菌（gftB基因）进行分子检测。最后，通过SPSS 27的统计方法估计细菌计数、分子发现和Snyder检验之间的关系。结果：各dmft组乳酸菌和变形链球菌的年龄及PCR结果差异有统计学意义（p < 0.05）。结论：该研究的结果可能被认为是一个有前途的和具有成本效益的筛查项目，以确定易患龋齿的儿童。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.