Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2024-12-24 Epub Date: 2024-12-10 DOI:10.1016/j.celrep.2024.115053

Attinder Chadha, Yu Yanai, Hiromu Oide, Yuichi Wakana, Hiroki Inoue, Saradindu Saha, Manish Paul, Mitsuo Tagaya, Kohei Arasaki, Shaeri Mukherjee

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引用次数: 0

Abstract

The bacterium Legionella pneumophila secretes numerous effector proteins that manipulate endoplasmic reticulum (ER)-derived vesicles to form the Legionella-containing vacuole (LCV). Despite extensive studies, whether the LCV membrane is separate from or connected to the host ER network remains unclear. Here, we show that the smooth ER (sER) is closely associated with the LCV early in infection. Remarkably, Legionella forms a distinct rough ER (rER) niche at later stages, disconnected from the host ER network. We discover that host small GTPases Rab10 and Rab4 and an ER protein, BAP31, play crucial roles in transitioning the LCV from an sER to an rER. Additionally, we have identified a Legionella effector, Lpg1152, that binds to BAP31. Interestingly, the optimal growth of Legionella is dependent on both BAP31 and Lpg1152. These findings detail the complex interplay between host and pathogen in transforming the LCV membrane from a host-associated sER to a distinct rER.

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军团菌利用宿主Rab GTPases和BAP31来创建一个独特的ER生态位。

嗜肺军团菌分泌大量效应蛋白，操纵内质网（ER）衍生的囊泡形成含军团菌液泡（LCV）。尽管有广泛的研究，LCV膜是否与宿主内质网分离或连接仍不清楚。在这里，我们发现平滑内质网（sER）在感染早期与LCV密切相关。值得注意的是，军团菌在后期形成了一个明显的粗糙ER （rER）生态位，与宿主ER网络断开。我们发现宿主小gtpase Rab10和Rab4以及ER蛋白BAP31在LCV从sER转化为rER的过程中发挥了关键作用。此外，我们已经鉴定出一种军团菌效应物Lpg1152，它与BAP31结合。有趣的是，军团菌的最佳生长同时依赖于BAP31和Lpg1152。这些发现详细说明了宿主和病原体之间复杂的相互作用，将LCV膜从宿主相关的sER转化为独特的rER。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.