Green surface modification of nylon membrane to site-specifically co-immobilize xylanase and lichenase for clean production of reducing sugar and juice clarification

IF 9.7 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Yanhong Zhou , Zijiao Yang , Yaxin Chen , Ruifang Zhang , Chun Yang , Wei Jiang , Guangya Zhang
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引用次数: 0

Abstract

The scarcity of simple and green functionalization protocols for carriers and the high purification cost of enzymes greatly hindered the application of multi-enzymes. Herein, a universal green and mild (25 °C, 1 h) carrier modification strategy for targeted immobilization of multi-enzymes without complex purification was proposed, in which only biocompatible tannic acid and Elastin-like polypeptides-SpyCatcher (ELPs-SC) were fed. The biofunctionalized nylon membranes (ELPs-SC-NMs) exhibited excellent stability and only 8.5% loss after being incubated at 4 °C for 15 days. Then, ELPs-SC-NMs were adopted to site-specifically immobilize target multi-enzymes (xylanase-SpyTag-lichenase, XTL) directly from the crude solution with the load of 46.2 μg/cm2. The retention of the XTL was up to 95.2% after 6 days of incubation at 4 °C, and the activity of xylanase in XTL was still 79.8% of the initial value after 18 days of storage, which was increased by 278.2% compared with free xylanase. In addition, the covalent immobilized XTL showed excellent reusability, which remained 74.7% (xylanase) and 77.5% (lichenase) of the initial activity after 8 uses, respectively. Moreover, the immobilized multi-enzymes were applied for clean production of reducing sugars from biomass, the substrate conversion rate and total reducing sugar content were close to those of free enzymes. They also exhibited more excellent catalytic properties than free ones in the clarification of fruit juices. The universality of our strategy was also demonstrated on poly(vinylidene fluoride) membranes. The green site-specific covalent immobilization avoids the complex enzyme purification process and significantly saves the cost, which will shield light both on co-immobilized multi-enzymes for cleaning biocatalytic applications and mild bio-based material surface functionalization.

Abstract Image

Abstract Image

对尼龙膜进行绿色表面改性,定点共固定木聚糖酶和地衣酶,用于清洁生产还原糖和果汁澄清
简单、绿色的载体功能化方案的缺乏和酶的高纯化成本极大地阻碍了多酶的应用。本文提出了一种通用的绿色温和(25°C, 1 h)载体修饰策略,用于多酶的靶向固定化,无需复杂的纯化,只投喂生物相容性单宁酸和弹性蛋白样多肽- spycatcher (ELPs-SC)。生物功能化尼龙膜(ELPs-SC- nms)在4°C孵育15天后具有良好的稳定性,仅损失8.5%。然后采用ELPs-SC-NMs直接从粗液中定点固定靶酶(木聚糖酶- spytag -地衣酶,XTL),负载为46.2 μg/cm2。在4℃条件下培养6 d后,XTL的保留率达到95.2%,贮藏18 d后,XTL的木聚糖酶活性仍为初始值的79.8%,比游离木聚糖酶提高了278.2%。此外,共价固定的XTL具有良好的重复利用性,使用8次后,木聚糖酶和地衣酶的活性分别保持在初始活性的74.7%和77.5%。此外,固定化多酶用于生物质清洁生产还原糖,其底物转化率和总还原糖含量与游离酶接近。它们在澄清果汁方面也表现出比游离的更优异的催化性能。我们的策略的普遍性也证明了聚偏氟乙烯膜。绿色位点特异性共价固定化避免了复杂的酶纯化过程,大大节省了成本,这将为清洁生物催化应用的共固定化多酶和温和的生物基材料表面功能化遮挡光。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Cleaner Production
Journal of Cleaner Production 环境科学-工程:环境
CiteScore
20.40
自引率
9.00%
发文量
4720
审稿时长
111 days
期刊介绍: The Journal of Cleaner Production is an international, transdisciplinary journal that addresses and discusses theoretical and practical Cleaner Production, Environmental, and Sustainability issues. It aims to help societies become more sustainable by focusing on the concept of 'Cleaner Production', which aims at preventing waste production and increasing efficiencies in energy, water, resources, and human capital use. The journal serves as a platform for corporations, governments, education institutions, regions, and societies to engage in discussions and research related to Cleaner Production, environmental, and sustainability practices.
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