Nanopaper Integrated Smart Device: An Opto-Electrochemical Biosensor for Real-Time Dual On-Field Detection of Organophosphorus Pesticides

IF 8.2 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Amir Reza Sharifi, Vincenzo Mazzaracchio, Leonardo Duranti, Ludovica Gullo, Simone Brannetti, Mohammad Peyravian, Mohammad Ali Kiani, Fabiana Arduini
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Abstract

The frequent and excessive use of organophosphorus pesticides in the agriculture industry raises persistent concerns regarding their environmental protection and public health implications. Addressing these issues requires the development of affordable and reliable sensing platforms for on-field monitoring to mitigate their adverse impacts promptly. This study utilizes nanocellulose papers (bacterial and TEMPO-oxidized) combined with butyrylcholinesterase to create a novel reagent-free and orthogonal nanobioplatform featuring smart opto-electrochemical dual outputs. An integrated nano-PAD, preloaded with enzymes and enzymatic substrates, is fabricated using wax-printing and screen-printing technologies. The nano-PAD measures opto-electroactive products, specifically indoxyl and thiocholine, whose concentrations correlate directly with the enzymatic inhibition caused by paraoxon, used as the organophosphate model. To enhance user convenience and meet the requirements for smart real-time point-of-need detection, integration of the nano-PAD with a smartphone-operated miniaturized potentiostat and a self-developed portable smart optical reader is achieved. The developed bioanalytical platform, further supported by a self-developed Android application, enables accurate and efficient quantification of dual signals in real time. The system covers a wide detection range of paraoxon (20–100 ppb) and demonstrates reliable recovery levels (ranging from 98 to 107%) in a real matrix, specifically wastewater. Given these demonstrated capabilities, this innovative biosensing strategy holds substantial potential for practical application in environmental surveillance, facilitating timely and informed environmental management decisions, particularly in resource-limited settings where traditional analytical tools are inaccessible.

Abstract Image

纳米纸集成智能装置:用于有机磷农药实时双现场检测的光电电化学生物传感器
在农业工业中频繁和过度使用有机磷农药引起了人们对其环境保护和公共健康影响的持续关注。解决这些问题需要开发负担得起和可靠的传感平台进行现场监测,以迅速减轻其不利影响。本研究利用纳米纤维素纸(细菌和tempo氧化)与丁基胆碱酯酶结合,创建了一种具有智能光电双输出的新型无试剂正交纳米生物平台。采用蜡印和丝网印刷技术制备了一种集成的纳米pad,预装有酶和酶底物。纳米pad测量光电活性产物,特别是吲哚和硫胆碱,其浓度与对氧磷引起的酶抑制直接相关,用作有机磷酸盐模型。为了提高用户的便利性,满足智能实时点检测的需求,实现了纳米pad与智能手机操作的小型化恒电位器和自主开发的便携式智能光学读取器的集成。开发的生物分析平台,进一步由自主开发的Android应用程序支持,可以实时准确高效地定量双信号。该系统覆盖了对氧磷的广泛检测范围(20-100 ppb),并在实际基质(特别是废水)中显示出可靠的回收率(范围从98到107%)。鉴于这些展示的能力,这种创新的生物传感战略在环境监测方面具有巨大的实际应用潜力,促进及时和知情的环境管理决策,特别是在资源有限的环境中,传统的分析工具是无法获得的。
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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