{"title":"Back to Basics: Unraveling the Fundamentals of Lateral Flow Assays.","authors":"Valentina Restrepo-Cano, Paola García-Huertas, Arley Caraballo-Guzmán, Miryan M Sánchez-Jiménez, Giovanny Torres-Lindarte","doi":"10.1093/jalm/jfae120","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Lateral flow assay (LFA) is a rapid analytical technique that has been implemented as a point-of-care approach for analyte detection. Given the rapid expansion of the use of LFA as a point-of-care testing strategy, LFA development has been subjected to extensive research, which has resulted in upgraded designs and technologies, improving levels of specificity and costs associated with manufacturing. This has allowed LFA to become an important option in rapid testing while maintaining appropriate limits of detection for accurate diagnoses.</p><p><strong>Content: </strong>This review focuses on the theoretical basis of LFA, its components, formats, multiparametric possibilities, labels, and applications. Also, challenges associated with the technique and possible solutions are explored.</p><p><strong>Summary: </strong>We explore LFA as a detection technique, its benefits, opportunities for improvement, and applications, and how challenges to its design can be approached.</p>","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Laboratory Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jalm/jfae120","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Lateral flow assay (LFA) is a rapid analytical technique that has been implemented as a point-of-care approach for analyte detection. Given the rapid expansion of the use of LFA as a point-of-care testing strategy, LFA development has been subjected to extensive research, which has resulted in upgraded designs and technologies, improving levels of specificity and costs associated with manufacturing. This has allowed LFA to become an important option in rapid testing while maintaining appropriate limits of detection for accurate diagnoses.
Content: This review focuses on the theoretical basis of LFA, its components, formats, multiparametric possibilities, labels, and applications. Also, challenges associated with the technique and possible solutions are explored.
Summary: We explore LFA as a detection technique, its benefits, opportunities for improvement, and applications, and how challenges to its design can be approached.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
