Screening and validation of downstream target genes of SLC25A21 based on bioinformatics.

Q3 Medicine
遗传 Pub Date : 2024-12-01 DOI:10.16288/j.yczz.24-230
Yao Chen, Xin Wen, Fang-Yuan Yuan, Chao-Ling Peng, Cui-Zhe Wang, Jun Zhang, Ping-Ping Meng
{"title":"Screening and validation of downstream target genes of SLC25A21 based on bioinformatics.","authors":"Yao Chen, Xin Wen, Fang-Yuan Yuan, Chao-Ling Peng, Cui-Zhe Wang, Jun Zhang, Ping-Ping Meng","doi":"10.16288/j.yczz.24-230","DOIUrl":null,"url":null,"abstract":"<p><p>Solute carrier 25 member 21 (<i>SLC25A21</i>) serves as an oxodicarboxylate carrier, which mainly conveys 2-oxoadipate from the cytoplasm to the mitochondria <i>via</i> a reverse exchange mechanism. Previous studies have indicated that the capacity for glucose consumption is significantly enhanced in 3T3-L1 cells overexpressing <i>SLC25A21</i>. In this study, we upregulate <i>SLC25A21</i> in 3T3-L1 cells to further probe into the downstream key metabolic genes of <i>SLC25A21</i>. Through high-throughput sequencing combined with bioinformatics analysis, differentially expressed genes are obtained, and the expression of key genes is verified by qRT-PCR. The results demonstrat that: (1) There are 26 up-regulated genes and 66 down-regulated genes in the adipocytes overexpressing <i>SLC25A21</i>; (2) GO (gene ontology) analysis indicates that the biological functions of differentially expressed genes are predominantly involved in lipid synthesis and metabolism, and KEGG (Kyoto encyclopedia of genes and genomes) and GSEA (gene set enrichment analysis) analyses reveal that differentially expressed genes are mainly concentrated in sphingolipid metabolism, secretion and synthesis of insulin and glucagon-like peptide 1; (3) By means of cytoHubba, 10 key genes with the highest scores, such as <i>GRB2, SOS1, SHC1, CBL, HRAS, SOS2, EGFR, MET, PLCG2</i> and <i>KRAS</i>, were screened out and they are mainly involved in the sugar and lipid metabolism processes of cells; (4) <i>SLC25A21</i> is overexpressed in adipocytes, and the qRT-PCR verification results show that the mRNA expression levels of other genes increased correspondingly, except for <i>KRAS</i> expression, which exhibits no significant change. These results provide a theoretical basis for further investigations on the role and mechanism of SLC25A21 in the process of glucose and lipid metabolism.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 12","pages":"1055-1065"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"遗传","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.16288/j.yczz.24-230","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Solute carrier 25 member 21 (SLC25A21) serves as an oxodicarboxylate carrier, which mainly conveys 2-oxoadipate from the cytoplasm to the mitochondria via a reverse exchange mechanism. Previous studies have indicated that the capacity for glucose consumption is significantly enhanced in 3T3-L1 cells overexpressing SLC25A21. In this study, we upregulate SLC25A21 in 3T3-L1 cells to further probe into the downstream key metabolic genes of SLC25A21. Through high-throughput sequencing combined with bioinformatics analysis, differentially expressed genes are obtained, and the expression of key genes is verified by qRT-PCR. The results demonstrat that: (1) There are 26 up-regulated genes and 66 down-regulated genes in the adipocytes overexpressing SLC25A21; (2) GO (gene ontology) analysis indicates that the biological functions of differentially expressed genes are predominantly involved in lipid synthesis and metabolism, and KEGG (Kyoto encyclopedia of genes and genomes) and GSEA (gene set enrichment analysis) analyses reveal that differentially expressed genes are mainly concentrated in sphingolipid metabolism, secretion and synthesis of insulin and glucagon-like peptide 1; (3) By means of cytoHubba, 10 key genes with the highest scores, such as GRB2, SOS1, SHC1, CBL, HRAS, SOS2, EGFR, MET, PLCG2 and KRAS, were screened out and they are mainly involved in the sugar and lipid metabolism processes of cells; (4) SLC25A21 is overexpressed in adipocytes, and the qRT-PCR verification results show that the mRNA expression levels of other genes increased correspondingly, except for KRAS expression, which exhibits no significant change. These results provide a theoretical basis for further investigations on the role and mechanism of SLC25A21 in the process of glucose and lipid metabolism.

求助全文
约1分钟内获得全文 求助全文
来源期刊
遗传
遗传 Medicine-Medicine (all)
CiteScore
2.50
自引率
0.00%
发文量
6699
期刊介绍: Hereditas is a national academic journal sponsored by the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences and the Chinese Society of Genetics and published by Science Press. It is a Chinese core journal and a Chinese high-quality scientific journal. The journal mainly publishes innovative research papers in the fields of genetics, genomics, cell biology, developmental biology, biological evolution, genetic engineering and biotechnology; new technologies and new methods; monographs and reviews on hot issues in the discipline; academic debates and discussions; experience in genetics teaching; introductions to famous geneticists at home and abroad; genetic counseling; information on academic conferences at home and abroad, etc. Main columns: review, frontier focus, research report, technology and method, resources and platform, experimental operation guide, genetic resources, genetics teaching, scientific news, etc.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信