The long noncoding RNA ALEX1 confers a functional phase state of ARF3 to enhance rice resistance to bacterial pathogens.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Meng-Qi Lei, Rui-Rui He, Yan-Fei Zhou, Lu Yang, Zhen-Fei Zhang, Chao Yuan, Wen-Long Zhao, Yu Cheng, Jian-Ping Lian, Yu-Chan Zhang, Wen-Tao Wang, Yang Yu, Yue-Qin Chen
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引用次数: 0

Abstract

Rice bacterial blight is a devastating disease worldwide, causing significant yield losses. Understanding how plants defend against microbial infection is critical for sustainable crop production. We previously identified a pathogen-induced long noncoding RNA (ALEX1). In this study, we show that ALEX1 localizes to the nucleus and directly binds to AUXIN RESPONSE FACTOR 3 (ARF3). We demonstrate that ARF3 forms condensates in the nucleus via its intrinsically disordered middle region (MR). Notably, ARF3 condensates takes on solid-like properties. We further revealed that ALEX1 directly binds to the MR of ARF3, regulating ARF3 condensate dynamics and promotes ARF3 homodimerization. The dispersed and dimeric form of ARF3, referred to as its functional phase state, enhances its ability to transcriptionally repress downstream target gene such as JAZ13, thereby modulating the jasmonic acid (JA) signaling pathway and strengthening pathogen resistance in rice. This study highlights the role of a long noncoding RNA in regulating protein condensation and assembly, contributing to pathogen defense in plants.

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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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