Histopathological and diagnostic aspects of glanders based on a case series from Brazil

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
A.F.C. Nassar , D.P. Chiebao , C. Del Fava , S. Miyashiro , V. Castro , R.A. Ogata , J.M. Yamamora , C.A.S. Monteiro , E.J.B. Monteiro
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引用次数: 0

Abstract

Glanders is a zoonotic disease of equids caused by the bacterium Burkholderia mallei, responsible for considerable economic loss. This study aimed to describe the clinical manifestations, pathological findings, and also bacteriological and molecular methods for agent detection in naturally infected animals (16 adult horses and one fetus) detected by serological survey from three glanders outbreaks. Of the 16 horses, 6 (37.5%) did not show clinical signs. After necropsy,samples of organs, lymph nodes, lesions and secretions were collectedfor histopathology, bacterial isolation, and PCR. The clinical and gross alterations mainly comprised nasal and respiratory forms in the three outbreaks, and less of the cutaneous form. All tested animals were positive by PCR (100%, n=17) on at least one sample. Of 121 samples analyzed 8.2% (10/121) isolated B. mallei and 41.3% (50/121) were polymerase chain reaction (PCR) positive. This work highlights the importance of combining diagnostic techniques, such as histopathology microbiological culture and PCR,to confirm cases and characterize the morbidity of glanders as well as considering seropositive animals without glanders clinical signs as potential carrier animals affecting disease control programmes.
组织病理学和诊断方面的腺体基于一个病例系列从巴西。
腺病是马科动物的一种人畜共患疾病,由马氏伯克霍尔德菌引起,造成相当大的经济损失。本研究通过血清学调查,对三次腺疫的自然感染动物(16匹成年马和1个胎儿)进行了临床表现、病理表现、细菌学和分子检测方法的研究。16匹马中,6匹(37.5%)无临床症状。尸检后取脏器、淋巴结、病变及分泌物标本进行组织病理学、细菌分离及PCR检测。临床和肉眼的改变主要包括鼻和呼吸形式的三次爆发,皮肤形式较少。所有实验动物至少有一个样本经PCR检测呈阳性(100%,n=17)。在121份样本中,8.2%(10/121)分离到马来芽孢杆菌,41.3%(50/121)聚合酶链反应(PCR)阳性。这项工作强调了结合诊断技术的重要性,如组织病理学微生物培养和聚合酶链反应,以确认病例和描述腺体发病率,以及考虑没有腺体临床症状的血清阳性动物作为影响疾病控制计划的潜在携带者动物。
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来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
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