Antibiotic-Resistant Pseudomonas aeruginosa from Market Meat in Peru.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-12-10 DOI:10.1089/fpd.2024.0052

Fernando Guibert, Beatriz Rojo-Bezares, Kathya Espinoza, Carla A Alonso, Rosario Oporto-Llerena, María López, Clara Taboada-Blanco, Yolanda Sáenz, Maria J Pons, Joaquim Ruiz

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引用次数: 0

Abstract

Unconsidered microorganisms, such as Pseudomonas aeruginosa, may be often recovered from food samples. This study aimed to characterize seven P. aeruginosa recovered from traditional market chicken meat in Lima, Peru. Antimicrobial susceptibility to 18 antimicrobial agents as well as the presence of amino acid changes in fluoroquinolone targets, 10 mediated colistin resistance (mcr) genes and integrons were analyzed. Clonal relationships were determined through pulsed-field gel electrophoresis (PFGE). Serotype by agglutination, multilocus sequence typing and the presence of 14 virulence factors (VFs) were established. Two isolates were multidrug-resistant, all being fluoroquinolone-resistant and exhibited the amino acid changes GyrA T₈₃I and ParC S₈₇L. No mcr gene was detected in the colistin-resistant isolates. The isolates showed identical PFGE patterns, and the selected P6 isolate belonged to the serotype O:4 and the sequence type 1800, and presented 12 VFs (all but exoU and exlA). The present study highlights the presence of multidrug and virulent P. aeruginosa in market chicken meat, and suggests cross-contamination during meat manipulation.

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秘鲁市场肉类中的耐抗生素铜绿假单胞菌。

未被考虑的微生物，如铜绿假单胞菌，可能经常从食品样品中回收。本研究旨在描述从秘鲁利马传统市场鸡肉中回收的7种铜绿假单胞菌。分析了18种抗菌药敏感性及氟喹诺酮类药物靶点、10个介导的粘菌素耐药（mcr）基因和整合子的氨基酸变化情况。通过脉冲场凝胶电泳（PFGE）确定克隆关系。通过凝集分型、多位点序列分型和14种毒力因子（VFs）的存在建立血清型。两株菌株均为多重耐药菌株，均对氟喹诺酮类药物耐药，并表现出氨基酸变化GyrA T83I和ParC S87L。耐粘菌素分离株未检出mcr基因。所选P6分离物均为o4血清型和1800序列型，存在12个VFs（除exoU和exlA外）。本研究强调市场鸡肉中存在多药毒性假单胞菌，并提示在肉类加工过程中存在交叉污染。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.