Application of Human Papillomavirus-DNA Test on Salivary Gland Fine Needle Aspiration Cytology Samples Confirms the Absence of the Virus in Primary Neoplasms and Demonstrates for the First Time Its Presence in Salivary Intraglandular Cysts

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Diagnostic Cytopathology Pub Date : 2024-12-10 DOI:10.1002/dc.25425

Immacolata Cozzolino, Andrea Ronchi, Marco Montella, Raffaella Ruggiero, Rosaria Cappiello, Giovanni Savarese, Giuseppe Colella, Renato Franco

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Abstract

Background

The correlation between Human Papillomavirus (HPV) and salivary gland neoplasms is still controversial. Data in the literature are conflicting, reporting the presence of the HPV-DNA in a significant percentage of cases or none. We investigated the presence of HPV in a series of salivary gland neoplasms using fine needle aspiration cytology (FNAC) samples to explore its potential oncogenic role in salivary gland tumor development.

Methods

The study included 66 salivary gland lesions from 65 patients. For all cases, residual cytological material in the needle hub, once direct smears were obtained, was fixed by rinsing the needle in Cytolyt hemolytic and preservative solution (Hologic Inc. Marlborough, MA 01752 USA). The samples fixed in the Cytolyt hemolytic and preservative solution (Hologic Inc. Marlborough, MA 01752 USA) were centrifuged at 252 g, then the sediment was transferred to the Preservcyt solution and used for the detection of HPV-DNA.

Results

We found evidence of the presence of HPV in two salivary intraglandular cysts lined by squamous cell epithelium. Furthermore, we also found HPV in three metastatic oropharyngeal squamous cell carcinomas (SCC) located in the parotid gland. Regarding primary salivary gland tumors, the HPV test produced negative results in all cases.

Conclusion

HPV testing produced negative results in all primary salivary gland tumors, failing to confirm the potential involvement of HPV infection in the pathogenesis of salivary gland tumors. Instead, the presence of HPV DNA in samples of salivary intraglandular cysts, never reported before, may be an interesting fact, which however requires further evaluation.

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人乳头瘤病毒dna检测在涎腺细针抽吸细胞学样本中的应用证实了该病毒在原发性肿瘤中的不存在，并首次证实了其在涎腺腺内囊肿中的存在。

背景：人乳头瘤病毒（HPV）与唾液腺肿瘤的相关性仍有争议。文献中的数据是相互矛盾的，报告HPV-DNA的存在在很大比例的病例中或没有。我们使用细针吸细胞学（FNAC）样本研究了HPV在一系列唾液腺肿瘤中的存在，以探索其在唾液腺肿瘤发展中的潜在致癌作用。方法：对65例患者66例唾液腺病变进行研究。对于所有病例，针头中心残留的细胞学物质，一旦获得直接涂片，通过在溶血和防腐剂溶液（Hologic Inc.）中冲洗针头来固定。Marlborough, MA 01752 USA)。样品固定在溶血和防腐剂溶液（Hologic Inc.）中。Marlborough, MA 01752 USA)离心252 g，然后将沉积物转移到Preservcyt溶液中，用于HPV-DNA的检测。结果：我们发现有证据表明HPV存在于两个涎腺内囊肿衬有鳞状细胞上皮。此外，我们还在三个位于腮腺的转移性口咽鳞状细胞癌（SCC）中发现了HPV。关于原发性唾液腺肿瘤，所有病例的HPV检测结果均为阴性。结论：所有原发性唾液腺肿瘤的HPV检测结果均为阴性，无法证实HPV感染可能参与唾液腺肿瘤的发病机制。相反，在唾液腺内囊肿样本中存在HPV DNA，以前从未报道过，可能是一个有趣的事实，但需要进一步评估。

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来源期刊

Diagnostic Cytopathology 医学-病理学

CiteScore

2.60

自引率

7.70%

发文量

163

审稿时长

3-6 weeks

期刊介绍： Diagnostic Cytopathology is intended to provide a forum for the exchange of information in the field of cytopathology, with special emphasis on the practical, clinical aspects of the discipline. The editors invite original scientific articles, as well as special review articles, feature articles, and letters to the editor, from laboratory professionals engaged in the practice of cytopathology. Manuscripts are accepted for publication on the basis of scientific merit, practical significance, and suitability for publication in a journal dedicated to this discipline. Original articles can be considered only with the understanding that they have never been published before and that they have not been submitted for simultaneous review to another publication.