Starvation changes the pre-rRNA accumulations in Caenorhabditis elegans

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-01-01 DOI:10.1016/j.bbrc.2024.151125

Shahriar Rahman Shovon , Tomoki Uematsu , Yuki Osaki , Tatsushi Masui , Takashi Koyama , Toshinobu Fujiwara , Chisato Ushida

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Abstract

The precursor rRNA (pre-rRNA) processing pathway in Caenorhabditis elegans remains unidentified. We have determined the cleavage site within the internal transcribed spacer 2, which generates pre-rRNA f of 5.8S rRNA and pre-rRNA g of 26S rRNA. Ribosome biogenesis is the most energetically demanding process of the cell. Under starved conditions, cells conserve energy by reconfiguring ribosome biogenesis to sustain proper growth. We investigated the short-term effects of starvation on the ribosomal RNA (rRNA) maturation in C. elegans. Northern blot analysis revealed significant changes in both small and large pre-rRNAs, with a substantial decrease in pre-rRNA c’1/27SA₂ of the large subunit and an increase in pre-rRNA d of the small subunit. Additionally, most pre-rRNAs accumulated in the nucleolar center, while the rRNA maturation factor FIB-1 accumulated at the nucleolar periphery. Nutritional replenishment restored the pre-rRNA accumulation pattern and the distribution of pre-rRNAs in the nucleolus to pre-starvation conditions.

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饥饿改变秀丽隐杆线虫的前rrna积累。

秀丽隐杆线虫前体rRNA （pre-rRNA）加工途径尚不清楚。我们确定了内部转录间隔器2的裂解位点，它产生5.8S rRNA的前rRNA f和26S rRNA的前rRNA g。核糖体的生物发生是细胞中最需要能量的过程。在饥饿条件下，细胞通过重新配置核糖体生物发生来保存能量，以维持正常的生长。我们研究了饥饿对秀丽隐杆线虫核糖体RNA成熟的短期影响。Northern blot分析显示，大小pre-rRNA均发生了显著变化，大亚基pre-rRNA c’1/27SA2显著减少，小亚基pre-rRNA d显著增加。此外，大多数前rRNA聚集在核仁中心，而rRNA成熟因子FIB-1聚集在核仁周围。营养补充使pre-rRNA的积累模式和核仁中pre-rRNA的分布恢复到饥饿前的状态。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics