Spatiotemporal evolution of heterogeneous structures in agarose gels revealed by particle tracking†

IF 2.9 3区 化学 Q3 CHEMISTRY, PHYSICAL
Soft Matter Pub Date : 2024-12-03 DOI:10.1039/D4SM01122H
Naoya Yanagisawa, Takemi Hara and Miho Yanagisawa
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引用次数: 0

Abstract

Upon decreasing the temperature, agarose solution exhibited gelation and phase separation, forming a cloudy gel consisting of agarose-rich and agarose-poor phases. Both phenomena contribute to the formation of a heterogeneous gel structure, but the primary influence of both processes on this heterogeneity remains unclear. In this study, we defined the specific gelation and phase separation temperatures of an agarose solution and examined the resulting gel structures with and without phase separation. Microscopic observation and colloid diffusion analysis revealed that phase separation leads to inhomogeneities several micrometers in size. Furthermore, we found that the distributions of colloidal diffusion coefficients and particle displacements strongly reflected the heterogeneity primarily induced by phase separation and gelation. Our findings contribute to the physicochemical understanding of the heterogeneous structures of various (bio) polymer gels associated with the phase separation of polymers.

Abstract Image

琼脂糖凝胶中非均相结构的时空演化
降低温度后,琼脂糖溶液发生凝胶化和相分离,形成由富琼脂糖相和贫琼脂糖相组成的浑浊凝胶。这两种现象都有助于形成非均质凝胶结构,但这两种过程对这种非均质性的主要影响尚不清楚。在这项研究中,我们定义了琼脂糖溶液的特定凝胶化和相分离温度,并检查了有和没有相分离的凝胶结构。显微观察和胶体扩散分析表明,相分离导致了几微米大小的不均匀性。此外,我们发现胶体扩散系数和颗粒位移的分布强烈地反映了主要由相分离和凝胶化引起的非均质性。我们的发现有助于对与聚合物相分离相关的各种(生物)聚合物凝胶的非均相结构的物理化学理解。
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来源期刊
Soft Matter
Soft Matter 工程技术-材料科学:综合
CiteScore
6.00
自引率
5.90%
发文量
891
审稿时长
1.9 months
期刊介绍: Soft Matter is an international journal published by the Royal Society of Chemistry using Engineering-Materials Science: A Synthesis as its research focus. It publishes original research articles, review articles, and synthesis articles related to this field, reporting the latest discoveries in the relevant theoretical, practical, and applied disciplines in a timely manner, and aims to promote the rapid exchange of scientific information in this subject area. The journal is an open access journal. The journal is an open access journal and has not been placed on the alert list in the last three years.
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