Junpeng Yu, Ze Wu, Xinyue Liu, Qianqian Fang, Xue Pan, Sujuan Xu, Man He, Jinxing Lin, Nianjun Teng
{"title":"LoBLH6 interacts with LoMYB65 to regulate anther development through feedback regulation of gibberellin synthesis in lily","authors":"Junpeng Yu, Ze Wu, Xinyue Liu, Qianqian Fang, Xue Pan, Sujuan Xu, Man He, Jinxing Lin, Nianjun Teng","doi":"10.1093/hr/uhae339","DOIUrl":null,"url":null,"abstract":"The homeostasis of gibberellin (GA) is crucial for the normal development of anthers, but its underlying regulatory mechanisms are not clear. The GA induced v-Myb myeloblastosis viral oncogene homolog (MYB) transcription factor LoMYB65 is involved in anther development. In this study, we screened and identified an interacting protein of LoMYB65, Lilium Oriental Hybrids BEL1-Like Homeodomain6 (LoBLH6). LoBLH6 was localized in both the nucleus and cytoplasm, and it interacted with LoMYB65 through its BELL domain, exhibiting transcriptional repression activity. LoBLH6 was continuously expressed during anther development, with particularly high expression in the mid and late stages. In situ hybridization revealed high expression of LoBLH6 in the tapetum and microspores, with the same tissue specificity as LoMYB65. Silencing of LoBLH6 in lilies resulted in abnormal anther development, reduced pollen, and increased GA content. The application of GA induced phenotypes in the anthers and pollen of lily that were similar to the silencing of LoBLH6. Further research showed that LoBLH6 directly binds to the promoter of Lilium Oriental Hybrids GA 20-oxidase1 (LoGA20ox1) to suppress its expression, and co-expression with LoMYB65 enhances this repression. Additionally, GA treatment enhanced the interaction between LoBLH6 and LoMYB65 and their complex's inhibitory effect on downstream target genes. During the transition from microspores to mature pollen grains in lily anthers, GA levels maintain a steady state, which is disrupted by silencing LoBLH6, leading to abnormal pollen development. Overall, our results reveal that the interaction between LoBLH6 and LoMYB65 regulates anther development through feedback regulation of GA synthesis.","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"154 1","pages":""},"PeriodicalIF":8.7000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/hr/uhae339","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
The homeostasis of gibberellin (GA) is crucial for the normal development of anthers, but its underlying regulatory mechanisms are not clear. The GA induced v-Myb myeloblastosis viral oncogene homolog (MYB) transcription factor LoMYB65 is involved in anther development. In this study, we screened and identified an interacting protein of LoMYB65, Lilium Oriental Hybrids BEL1-Like Homeodomain6 (LoBLH6). LoBLH6 was localized in both the nucleus and cytoplasm, and it interacted with LoMYB65 through its BELL domain, exhibiting transcriptional repression activity. LoBLH6 was continuously expressed during anther development, with particularly high expression in the mid and late stages. In situ hybridization revealed high expression of LoBLH6 in the tapetum and microspores, with the same tissue specificity as LoMYB65. Silencing of LoBLH6 in lilies resulted in abnormal anther development, reduced pollen, and increased GA content. The application of GA induced phenotypes in the anthers and pollen of lily that were similar to the silencing of LoBLH6. Further research showed that LoBLH6 directly binds to the promoter of Lilium Oriental Hybrids GA 20-oxidase1 (LoGA20ox1) to suppress its expression, and co-expression with LoMYB65 enhances this repression. Additionally, GA treatment enhanced the interaction between LoBLH6 and LoMYB65 and their complex's inhibitory effect on downstream target genes. During the transition from microspores to mature pollen grains in lily anthers, GA levels maintain a steady state, which is disrupted by silencing LoBLH6, leading to abnormal pollen development. Overall, our results reveal that the interaction between LoBLH6 and LoMYB65 regulates anther development through feedback regulation of GA synthesis.
期刊介绍:
Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.