A Feasible Regulation Strategy for Conjugation of Antibiotic Resistance Genes Based on Different Bacterial Quorum Sensing Inhibition Methods

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2024-12-10 DOI:10.1016/j.watres.2024.122958

Chen-Yi Jiang, Xiao-Chi Feng, Hong-Tao Shi, Shu-Hong Gao, Wen-Qian Wang, Zi-Jie Xiao, Nan-Qi Ren

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Abstract

The dissemination of antibiotic resistance genes (ARGs) poses global environmental issues, and plasmid-mediated conjugation contributes substantially to the spread of ARGs. Quorum sensing (QS), an important cell–cell communication system that coordinates group behaviors, has potential as a feasible regulation pathway to inhibit the conjugation process. We examined the promoting effects of QS signal on conjugation, and this study is the first to report that QS inhibitors 2(3H)-benzofuranone and acylase I effectively repressed conjugation frequency of RP4 plasmid to 0.32- and 0.13-fold compared with the control respectively. The investigation of underlying mechanisms of QS inhibitors revealed a significant decrease in cellular contact and the formation of transfer channels. The downregulation of sdiA gene regulating the expression of QS signal receptor contribute to conjugation inhibition. Importantly, the expression of genes related to the formation of conjugative pili, which plays a role in plasmid mating bridge formation was downregulated, indicating QS inhibitors affect conjugation mainly through regulation of the mating pair formation system. Furthermore, 2(3H)-benzofuranone and acylase I achieved 84.07% and 66.05% inhibitory effect on plasmid spread in activated sludge reactors. Collectively, our findings demonstrate the feasibility of using different bacteria quorum quenching methods to control the spread of ARGs.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.