VvNAC17, a grape NAC transcription factor, regulates plant response to drought-tolerance and anthocyanin synthesis.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI:10.1016/j.plaphy.2024.109379

Zi-Lan Jin, Wan-Ni Wang, Qiong Nan, Jia-Wen Liu, Yan-Lun Ju, Yu-Lin Fang

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引用次数: 0

Abstract

NAC transcription factors are unique to plants and play a role in stress. In this study, the VvNAC17 gene was isolated from grapes, and tested the functions of VvNAC17 under drought stress. The expression level of VvNAC17 in callus could be highly induced by drought stress. VvNAC17 overexpression in callus conferred drought-resistant phenotypes with lower MDA content, higher antioxidant enzyme activity (CAT, POD, SOD), higher monomer anthocyanin content, and higher expression levels of some drought-related genes (VvDREB1A, VvDREB2A, VvDREB2D, VvRD29A, VvPIN5) and anthocyanin-biosynthesis-related genes (VvUFGT, VvANS, VvANR, VvDFR,VvLAR). Meanwhile, the Y1H and Dual-LUC assays showed that VvNAC17 could activate VvDREB1A and VvUFGT expression by binding to its promoter. Futhermore, RNA-seq showed that VvNAC17 can affect grape growth and development by affecting the photosynthesis and metabolism of some macromolecules. Taken together, the NAC transcription factor VvNAC17 could positively regulates drought-tolerance. VvNAC17 is a promising candidate for improving drought resistance in grape.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.