Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells.

IF 1.7 4区 生物学 Q3 BIOLOGY
Open Life Sciences Pub Date : 2024-11-26 eCollection Date: 2024-01-01 DOI:10.1515/biol-2022-1000
Bala Baskaran Durga, Vinayagam Ramachandran, Bakthavatchalam Senthil, Vasthi Gnanarani Soloman, Mohamed Soliman Elshikh, Saeedah Musaed Almutairi, Zhi-Hong Wen, Yi-Hao Lo
{"title":"Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells.","authors":"Bala Baskaran Durga, Vinayagam Ramachandran, Bakthavatchalam Senthil, Vasthi Gnanarani Soloman, Mohamed Soliman Elshikh, Saeedah Musaed Almutairi, Zhi-Hong Wen, Yi-Hao Lo","doi":"10.1515/biol-2022-1000","DOIUrl":null,"url":null,"abstract":"<p><p>This research examines the cytotoxic consequences of thymoquinone-loaded bovine serum albumin nanoparticles (TQ-BSA NPs) on the A549 lung cancer cell line. UV-visible (UV-Vis) spectroscopy, Fourier transform infrared spectrophotometer (FT-IR), scanning electron microscopy (SEM), and dynamic light scattering (DLS) were employed to verify the biogenic TQ-BSA NPs' size, shape, and distribution. UV-Vis spectrophotometry indicated peaks at 200-300 nm, 500-600 nm, and a prominent peak at 700-800 nm, confirming the presence of TQ-BSA NPs. The polydispersity index, as confirmed by DLS, indicated a solvent distribution in water, accompanied by a zeta potential value of 126.2 ± 46.8 mV. The average size of TQ-BSA NPs was confirmed to be 187 ± 8 nm by SEM. TQ-BSA NPs reduce colony formation in the A549 lung cancer cell line in a dose-dependent manner relative to the control group. Protein expression analysis indicated that TQ-BSA NPs promoted programmed cell death by increasing pro-apoptotic levels and decreasing anti-apoptotic levels. TQ-BSA NPs demonstrated inhibition of cancer cell proliferation and promotion of apoptosis and exhibited significant efficacy against cancer cells at low concentrations. As a result, they have the makings of a promising chemotherapeutic agent for low-dose, long-term administration.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221000"},"PeriodicalIF":1.7000,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11627060/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Life Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1515/biol-2022-1000","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

This research examines the cytotoxic consequences of thymoquinone-loaded bovine serum albumin nanoparticles (TQ-BSA NPs) on the A549 lung cancer cell line. UV-visible (UV-Vis) spectroscopy, Fourier transform infrared spectrophotometer (FT-IR), scanning electron microscopy (SEM), and dynamic light scattering (DLS) were employed to verify the biogenic TQ-BSA NPs' size, shape, and distribution. UV-Vis spectrophotometry indicated peaks at 200-300 nm, 500-600 nm, and a prominent peak at 700-800 nm, confirming the presence of TQ-BSA NPs. The polydispersity index, as confirmed by DLS, indicated a solvent distribution in water, accompanied by a zeta potential value of 126.2 ± 46.8 mV. The average size of TQ-BSA NPs was confirmed to be 187 ± 8 nm by SEM. TQ-BSA NPs reduce colony formation in the A549 lung cancer cell line in a dose-dependent manner relative to the control group. Protein expression analysis indicated that TQ-BSA NPs promoted programmed cell death by increasing pro-apoptotic levels and decreasing anti-apoptotic levels. TQ-BSA NPs demonstrated inhibition of cancer cell proliferation and promotion of apoptosis and exhibited significant efficacy against cancer cells at low concentrations. As a result, they have the makings of a promising chemotherapeutic agent for low-dose, long-term administration.

百里醌-牛血清白蛋白纳米颗粒对A549肺癌细胞的细胞毒作用。
本研究探讨了载百里醌的牛血清白蛋白纳米颗粒(TQ-BSA NPs)对A549肺癌细胞系的细胞毒性后果。采用紫外可见光谱(UV-Vis)、傅里叶变换红外分光光度计(FT-IR)、扫描电镜(SEM)和动态光散射(DLS)等方法验证了生物源TQ-BSA NPs的大小、形状和分布。紫外可见分光光度法在200 ~ 300 nm、500 ~ 600 nm处有一个峰,在700 ~ 800 nm处有一个显著峰,证实了TQ-BSA NPs的存在。DLS测定的多分散性指数表明其在水中呈溶剂分布,zeta电位值为126.2±46.8 mV。扫描电镜证实TQ-BSA NPs的平均尺寸为187±8 nm。与对照组相比,TQ-BSA NPs在A549肺癌细胞系中以剂量依赖的方式减少集落形成。蛋白表达分析表明,TQ-BSA NPs通过增加促凋亡水平和降低抗凋亡水平促进程序性细胞死亡。TQ-BSA NPs具有抑制癌细胞增殖和促进细胞凋亡的作用,在低浓度下具有显著的抗癌作用。因此,它们具有低剂量、长期给药的有前途的化疗药物的成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.50
自引率
4.50%
发文量
131
审稿时长
43 weeks
期刊介绍: Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信