Electroacupuncture alleviates cerebral ischemia injury by regulating PI3K/AKT/NF-κB signaling in microglia of ischemic stroke rats.

IF 1.6 4区医学 Q4 NEUROSCIENCES

Neuroreport Pub Date : 2025-01-08 Epub Date: 2024-12-04 DOI:10.1097/WNR.0000000000002115

Rao Rao, Lingling Gan, Rui Zhao, Yongsheng Han

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引用次数: 0

Abstract

This study aimed to investigate the potential of electroacupuncture as an intervention for inducing 'Awakening and Opening of the Brain' in rats with stroke models induced by middle cerebral artery occlusion/reperfusion (MCAO/R). The efficacy of electroacupuncture in alleviating cerebral ischemic injury was evaluated using Longa scores, triphenyl tetrazolium chloride staining, and hematoxylin and eosin staining. Non-targeted metabolomics analysis was conducted to identify differential metabolite changes before and after electroacupuncture treatment in MCAO/R rats. Network pharmacology analysis was then performed to correlate these differential metabolites with ischemic stroke. The PI3K/AKT/NF-κB signaling pathway was identified as a key target. In vivo experiments further validated the mechanism by which electroacupuncture promotes M2 microglial polarization through inhibition of the PI3K/AKT/NF-κB signaling in MCAO/R rats. This study demonstrated that electroacupuncture reduces brain damage and inhibits inflammation in MCAO/R rats by modulating the PI3K/AKT/NF-κB signaling pathway and promoting the polarization of microglia from M1 to M2.

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电针通过调节缺血性脑卒中大鼠小胶质细胞PI3K/AKT/NF-κB信号通路减轻脑缺血损伤。

本研究旨在探讨电针在大脑中动脉闭塞/再灌注（MCAO/R）脑卒中模型大鼠中诱导“脑觉醒和开放”的干预作用。采用Longa评分、氯化三苯四唑染色、苏木精和伊红染色评价电针缓解脑缺血损伤的疗效。通过非靶向代谢组学分析，确定电针治疗MCAO/R大鼠前后代谢物的差异变化。然后进行网络药理学分析，将这些差异代谢物与缺血性中风联系起来。PI3K/AKT/NF-κB信号通路被确定为关键靶点。体内实验进一步验证了电针通过抑制MCAO/R大鼠PI3K/AKT/NF-κB信号通路促进M2小胶质细胞极化的机制。本研究表明，电针通过调节PI3K/AKT/NF-κB信号通路，促进小胶质细胞从M1向M2极化，减轻MCAO/R大鼠脑损伤，抑制炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.