Is dragging a drag or is trapping a trap? A comparison of two methods for collecting Amblyomma americanum ticks in sites near the species range boundary.

Abstract

The incidence of tick-borne diseases in the United States has more than doubled since the early 2000s. Research on ticks is a priority to mitigate the spread of tick-borne diseases. Thus, it is important to understand how to efficiently collect large numbers of ticks for studies of genetics, behavior, physiology, vector competence, tick repellants, and acaricides. In this study, we compared the efficiency of two methods-dragging and CO₂-baited trapping-for collecting lone star ticks (Amblyomma americanum) across two distinct regions of its expanding range. We performed simultaneous dragging and trapping collections at six sites, three in Oklahoma and three in New York and New Jersey, USA. Our results demonstrate that dragging was more efficient than trapping for collecting lone star tick nymphs. However, dragging and trapping were similar in efficiency for collecting adult males and females. There were no regional differences in trapping or dragging efficiency. Additionally, we discuss material and labor costs of each method to inform researchers who need to rapidly collect as many ticks as possible with the most efficient and cost-effective method.