Fabrication and Characterization of Agar- and Seaweed-Derived Biomembrane Films for Biomedical and Other Applications

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biopolymers Pub Date : 2024-12-10 DOI:10.1002/bip.23643

Muthiyal Prabakaran Sudhakar, Sureshkumar Ambika Nived, Gopal Dharani

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引用次数: 0

Abstract

This study focused on seaweed-based biomembrane development. The physical, mechanical, thermal, and biological properties of the fabricated films with different combinations of materials, such as agar, chitosan, poly(vinyl) alcohol (PVA), and quercetin, were characterized. The surface morphology of the films was analyzed using SEM. The maximum tensile strength (53.11 N/mm²), elongation at break (3.42%), and Young's modulus (15.52) of the biomembrane were recorded for the agar + chitosan combination. FT-Raman analysis confirmed the functional groups shift between the biopolymer and plasticizer used in this study. TG-DSC analysis of the biomembranes revealed a T_g in the range of 92.80°C–115°C. The maximum antioxidant activity was reported for quercetin (58.62%), and the maximum antimicrobial activity was observed for the chitosan and quercetin compounds against E. coli. A minimum hemolysis of 0.95% was achieved for the combination of agar + quercetin (AQ), agar + PEG (APE), Gracilaria corticata extract + PVA + quercetin (GCPQ) and agar + chitosan (AC) biomembranes. The minimum cytotoxicity of the biomembrane was 62.51% and 63.87% for Gracilaria corticata extract + PVA + quercetin (GCPQ), and agar + PVA, respectively. The proposed biomembrane films were found to be suitable for biomedical and packaging applications.

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用于生物医学和其他应用的琼脂和海藻衍生生物膜薄膜的制备和表征。

本研究的重点是海藻基生物膜的开发。考察了琼脂、壳聚糖、聚乙烯醇、槲皮素等不同材料组合制备的薄膜的物理、机械、热、生物性能。利用扫描电镜对膜的表面形貌进行了分析。琼脂+壳聚糖复合生物膜的最大拉伸强度为53.11 N/mm2，断裂伸长率为3.42%，杨氏模量为15.52。FT-Raman分析证实了本研究中使用的生物聚合物和增塑剂之间的官能团转移。生物膜的Tg - dsc分析显示Tg在92.80°C-115°C之间。槲皮素的抗氧化活性最高（58.62%），壳聚糖和槲皮素对大肠杆菌的抑菌活性最高。琼脂+槲皮素（AQ）、琼脂+ PEG （APE）、桂花提取物+ PVA +槲皮素（GCPQ）和琼脂+壳聚糖（AC）生物膜的溶血率最低为0.95%。皮葛提取物+ PVA +槲皮素（GCPQ）和琼脂+ PVA生物膜的最小细胞毒性分别为62.51%和63.87%。所提出的生物膜薄膜被发现适用于生物医学和包装应用。

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来源期刊

Biopolymers 生物-生化与分子生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Founded in 1963, Biopolymers publishes strictly peer-reviewed papers examining naturally occurring and synthetic biological macromolecules. By including experimental and theoretical studies on the fundamental behaviour as well as applications of biopolymers, the journal serves the interdisciplinary biochemical, biophysical, biomaterials and biomedical research communities.