A pilot study of the value of micronucleus count in urinary cytology samples in the follow-up of patients with urothelial carcinoma: Implications for diagnosis and prognosis

IF 2.6 3区医学 Q3 ONCOLOGY

Cancer Cytopathology Pub Date : 2024-12-10 DOI:10.1002/cncy.22923

Tuba Dilay Kökenek Ünal MD, Ayşegül Aksoy Altınboğa MD

{"title":"A pilot study of the value of micronucleus count in urinary cytology samples in the follow-up of patients with urothelial carcinoma: Implications for diagnosis and prognosis","authors":"Tuba Dilay Kökenek Ünal MD, Ayşegül Aksoy Altınboğa MD","doi":"10.1002/cncy.22923","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Nuclear protrusions such as micronuclei (MNs) and nuclear budding (NB) are morphological findings of chromosomal instability and indicators of genotoxic damage. They are increased in malignancies, and their high frequency may be used in the diagnosis of cancers and the follow-up of patients. Urothelial carcinomas are common tumors that cause morbidity and mortality, and cytology is a commonly used method for the monitoring and screening of urothelial carcinoma. Although the cytological evaluation of urinary samples is mainly based on nuclear features, there is limited research focusing on MN frequency in urinary cytology. This study aimed to investigate MN and NB counts in various diagnostic categories of urinary samples.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This study included 117 urinary cytology samples categorized according to The Paris System for Reporting of Urinary Cytology. Two observers, blinded to the diagnosis, counted the frequency of MNs and NB per 1000 cells on May-Grünwald-Giemsa– and Papanicolaou-stained slides.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>MN and NB counts significantly differed among the groups (<i>p</i> < .001 for each) with a large effect (Ɛ<sup>2</sup> = 0.509). MN and NB counts were significantly higher in cases with high-grade urothelial carcinoma (HGUC) than in control cases and in cases that were negative for HGUC or with atypical urothelial cells (<i>p</i> < .001 for each). Any MN count greater than 2.5 per 1000 cells indicated HGUC with a 55% sensitivity and 92.4% specificity.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Because increased MN and NB frequencies are closely associated with an increased risk of malignancy, these could be integrated into The Paris System for Reporting of Urinary Cytology.</p>\n </section>\n </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":"133 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Cytopathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cncy.22923","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Nuclear protrusions such as micronuclei (MNs) and nuclear budding (NB) are morphological findings of chromosomal instability and indicators of genotoxic damage. They are increased in malignancies, and their high frequency may be used in the diagnosis of cancers and the follow-up of patients. Urothelial carcinomas are common tumors that cause morbidity and mortality, and cytology is a commonly used method for the monitoring and screening of urothelial carcinoma. Although the cytological evaluation of urinary samples is mainly based on nuclear features, there is limited research focusing on MN frequency in urinary cytology. This study aimed to investigate MN and NB counts in various diagnostic categories of urinary samples.

Methods

This study included 117 urinary cytology samples categorized according to The Paris System for Reporting of Urinary Cytology. Two observers, blinded to the diagnosis, counted the frequency of MNs and NB per 1000 cells on May-Grünwald-Giemsa– and Papanicolaou-stained slides.

Results

MN and NB counts significantly differed among the groups (p < .001 for each) with a large effect (Ɛ² = 0.509). MN and NB counts were significantly higher in cases with high-grade urothelial carcinoma (HGUC) than in control cases and in cases that were negative for HGUC or with atypical urothelial cells (p < .001 for each). Any MN count greater than 2.5 per 1000 cells indicated HGUC with a 55% sensitivity and 92.4% specificity.

Conclusions

Because increased MN and NB frequencies are closely associated with an increased risk of malignancy, these could be integrated into The Paris System for Reporting of Urinary Cytology.

查看原文本刊更多论文

尿路上皮癌患者随访中尿细胞学样本微核计数价值的初步研究：对诊断和预后的影响。

背景：核突出如微核（MNs）和核出芽（NB）是染色体不稳定的形态学表现和基因毒性损伤的指标。它们在恶性肿瘤中增加，其高频率可用于癌症的诊断和患者的随访。尿路上皮癌是引起发病率和死亡率的常见肿瘤，细胞学是监测和筛查尿路上皮癌的常用方法。虽然尿液样本的细胞学评价主要基于核特征，但对尿液细胞学中MN频率的研究有限。本研究旨在探讨不同诊断类别尿液样本中MN和NB计数。方法：本研究纳入117例泌尿细胞学样本，按巴黎泌尿细胞学报告系统分类。两名不知道诊断结果的观察者在may - gr nwald- giemsa和papanicolao染色玻片上计算每1000个细胞中MNs和NB的频率。结果：组间MN、NB计数差异有统计学意义（p 2 = 0.509）。高级别尿路上皮癌（HGUC）患者的MN和NB计数明显高于对照组和HGUC阴性或非典型尿路上皮细胞的患者(p结论：由于MN和NB频率的增加与恶性肿瘤风险的增加密切相关，这些可以整合到巴黎尿细胞学报告系统中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cancer Cytopathology 医学-病理学

CiteScore

7.00

自引率

17.60%

发文量

130

审稿时长

1 months

期刊介绍： Cancer Cytopathology provides a unique forum for interaction and dissemination of original research and educational information relevant to the practice of cytopathology and its related oncologic disciplines. The journal strives to have a positive effect on cancer prevention, early detection, diagnosis, and cure by the publication of high-quality content. The mission of Cancer Cytopathology is to present and inform readers of new applications, technological advances, cutting-edge research, novel applications of molecular techniques, and relevant review articles related to cytopathology.