Amphiphilic self-assembling peptides: formulation and elucidation of functional nanostructures for imaging and smart drug delivery.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Alice Am, Laura Trapiella-Alfonso, Charlotte Izabelle, Bruno Saubamea, Bich-Thuy Doan, Fanny d'Orlyé, Anne Varenne
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引用次数: 0

Abstract

The rational design of self-assembled peptide-based nanostructures for theranostics applications requires in-depth physicochemical characterization of the peptide nanostructures, to understand the mechanism and the interactions involved in the self-assembly, allowing a better control of the objects' physicochemical and functional properties for theranostic applications. In this work, several complementary characterization methods, such as dynamic light scattering, transmission electron microscopy, circular dichroism, Taylor dispersion analysis, and capillary electrophoresis, were used to study and optimize the self-assembly of pH-sensitive short synthetic amphiphilic peptides containing an RGD motif for active targeting of tumor cells and smart drug delivery. The combined methods evidenced the spontaneous formation of nanorods (L = 50 nm, d = 10 nm) at pH 11, stabilized by β-sheets. To complement with imaging properties for diagnosis, a new strategy was developed by designing an optimized peptide sequence to allow for efficient functionalization with a contrast agent, while preserving the self-assembling properties. Co-assemblies of the peptide and its derivatives, after peptide modification with a gadolinium complex, exhibited similar nanorod structures and required properties for drug delivery and imaging applications in vivo.

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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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