The Effect of Copper Limitation on the Bioavailability of Cu-Organic Complexes

IF 10.8 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Meizhen Li, Liangliang Kong, Neil M. Price
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Abstract

We examined the bioavailability of synthetic organic Cu complexes (Cu-L) and inorganic Cu species (Cu′) to Thalassiosira oceanica growing under Cu-limiting and Cu-inhibiting conditions. Copper bioavailability depended on the phytoplankton Cu nutritional state and whether Cu′ diffusion to the cell surface was sufficient to meet the cellular demands for growth. Under Cu-limiting conditions when [Cu′] was less than the diffusion concentration threshold (DCT: 10–14.13 M), growth rate was a hyperbolic function of [Cu-L]. Increasing [Cu′] above the DCT caused growth rate to increase proportionally, but growth rate also increased with increasing [Cu-L] so that both Cu′ and Cu-L were bioavailable. Short-term photosynthesis assays conducted under Cu-limiting conditions showed a similar response to Cu speciation. In contrast, the growth rate of Cu-inhibited cells at high [Cu′] varied inversely with [Cu2+] and was independent of [Cu-L], as previously reported. The change in Cu-L bioavailability correlated with expression levels of genes encoding the reduction-dependent, high-affinity Cu uptake pathway, which was regulated by [Cu′]. Our analysis shows that at [Cu′] typical of the open sea, T. oceanica-like phytoplankton are diffusion limited and must rely on Cu-organic complexes to fulfill their Cu requirements for growth.

Abstract Image

铜限制对cu -有机配合物生物利用度的影响
研究了合成有机Cu配合物(Cu- l)和无机Cu种(Cu ')对生长在Cu限制和Cu抑制条件下的海洋盐藻的生物利用度。铜的生物利用度取决于浮游植物的Cu营养状态以及Cu向细胞表面的扩散是否足以满足细胞生长的需要。在Cu限制条件下,当[Cu ']小于扩散浓度阈值(DCT: 10-14.13 M)时,生长速率与[Cu- l]呈双曲函数关系。在DCT以上增加[Cu ‘]使生长速率成比例增加,但随着[Cu- l]的增加,生长速率也随之增加,从而使Cu ’和Cu- l都是生物可利用的。在Cu限制条件下进行的短期光合作用试验显示出对Cu形态形成的类似响应。与此相反,在高[Cu ']条件下,Cu抑制细胞的生长速率与[Cu2+]成反比,与[Cu- l]无关。Cu- l生物利用度的变化与[Cu ']调控的还原依赖性高亲和力Cu摄取途径编码基因的表达水平相关。我们的分析表明,在典型的公海[Cu ']中,T. oceanica样浮游植物的扩散有限,必须依靠Cu有机配合物来满足其生长对Cu的需求。
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来源期刊
环境科学与技术
环境科学与技术 环境科学-工程:环境
CiteScore
17.50
自引率
9.60%
发文量
12359
审稿时长
2.8 months
期刊介绍: Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences. Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.
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