Aliphatic substrates-mediated unique rapid room temperature synthesis of carbon quantum dots for fenofibrate versatile analysis

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Ali Abdel-Hakim , Fathalla Belal , Mohamed A. Hammad , Abdelaziz Elgaml , Mahmoud El-Maghrabey
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引用次数: 0

Abstract

Background

The current synthetic strategies for carbon dots (CDs) are usually time-consuming, rely on complicated processes, and need high temperatures and energy. Recent studies have successfully synthesized CDs at room temperature. Unfortunately, most CDs synthesized at room temperature are obtained under harsh reaction conditions, prepared using aromatic precursors, or need a long time to generate. Therefore, an energy-free room-temperature rapid synthesis of CDs under mild conditions using aliphatic substrates is important. We aim to provide an innovative approach to synthesizing CDs to be used to develop the first fluorescence-based assay of the non-fluorescent anti-hyperlipidemic drug, fenofibrate.

Results

We report an innovative, energy-free, and room-temperature preparation of fluorescent N-doped CDs utilizing aliphatic substrates in only 20 min. The synthesis was based on a self-exothermic Schiff base condensation reaction between methylglyoxal and ethylenediamine. The prepared CDs' antibacterial properties, biocompatibility, and cell-imaging ability were investigated. The fluorescence signal of the CDs was quantitively quenched upon adding increasing concentrations of fenofibrate in the range of 0.50–15.0 μg/mL. Therefore, the prepared CDs were applied as a nanosensor to develop the first fluorescence-based assay of fenofibrate. The reliability of the synthesized nanosensor was confirmed by the successful quantification of fenofibrate in pharmaceutical dosage forms, environmental water, weight loss herbal products, and dietary supplements. The obtained recovery ranged from 95.33 to 104.58 %. In addition, the minimal environmental impact of the developed fenofibrate sensing strategy was confirmed using the recently reported metrics.

Significance and novelty

The key advantage of this work is the use of an energy-free approach to synthesize CDs rapidly under mild conditions without aromatic substrates. This opens a new window for the eco-friendly synthesis of CDs that avoids the drawbacks of the traditional methods. Additionally, it is the first fluorescence nanosensor for sensing fenofibrate in various matrices, avoiding the limitations of the previous methods, such as high cost, poor selectivity, and low sensitivity.

Abstract Image

Abstract Image

脂肪族底物介导的非诺贝特通用分析碳量子点的独特快速室温合成
目前的碳点合成方法通常耗时长、工艺复杂、需要高温和能量。最近的研究已经成功地在室温下合成了CDs。不幸的是,大多数在室温下合成的CDs是在苛刻的反应条件下获得的,使用芳香前体制备,或者需要很长时间才能生成。因此,利用脂肪族底物在温和条件下无能量的室温下快速合成CDs是很重要的。我们的目标是提供一种创新的方法来合成CDs,用于开发非荧光抗高脂血症药物非诺贝特的第一个基于荧光的检测。我们报告了一种创新的、无能量的、室温下利用脂肪族底物制备荧光n掺杂CDs的方法,仅需20分钟。甲基乙二醛与乙二胺通过自放热希夫碱缩合反应合成。研究了制备的CDs的抗菌性能、生物相容性和细胞成像能力。在0.50 ~ 15.0 μg/mL范围内增加非诺贝特浓度,定量猝灭CDs的荧光信号。因此,制备的CDs作为纳米传感器应用于非诺贝特的首次荧光检测。通过对非诺贝特在药物剂型、环境水、减肥草药产品和膳食补充剂中的定量分析,证实了所合成纳米传感器的可靠性。回收率为95.33 ~ 104.58%。此外,利用最近报告的指标证实了开发的非诺贝特传感策略的最小环境影响。本研究的主要优点是利用无能量的方法在温和的条件下快速合成CDs,而不需要芳香族底物。这为避免传统方法的缺点的环保合成cd打开了新的窗口。此外,它是第一个用于在各种基质中检测非诺贝特的荧光纳米传感器,避免了以往方法的局限性,如成本高、选择性差和灵敏度低。
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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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