Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2024-12-10 DOI:10.1007/s11104-024-07133-4

Junlong Xu, Yudie Shao, Xinhao Rao, Jinliang Tang, Christopher Rensing, Hend Alwathnani, Madeha Ayed Alonazi, Jinlin Zhang, Liming Zhang, Shihe Xing, Wenhao Yang

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引用次数: 0

Abstract

Background and aims

Silicon (Si) has been shown to alleviate cadmium (Cd) toxicity in plants, but the effect of Si on hyperaccumulators such as S. alfredii has not been fully elucidated. This study evaluated the impact of exogenous Si on growth, Cd accumulation, and rhizosphere properties of S. alfredii.

Methods

Si was applied to S. alfredii and various parameters were assessed, including biomass, shoot Cd content, chlorophyll, carotenoid levels, malondialdehyde (MDA) content, antioxidant enzyme activity, cell wall composition, soil nutrient availability, and rhizosphere enzyme activities. A thorough transcriptomic analysis was also conducted.

Results

Si significantly increased shoot biomass (21.47–104.35%) and Cd accumulation (144.60–747.11%) in S. alfredii. Si also enhanced chlorophyll levels, reduced the malondialdehyde (MDA) content, increased antioxidant enzyme activities, and mitigated Cd-induced oxidative stress. Si boosted cell wall components, thereby facilitating Cd transport in roots. In rhizosphere soil, Si improved alkaline hydrolysis of nitrogen (AHN), available phosphorus (AP), available potassium (AK), dissolved organic carbon (DOC), and increased available Cd content. Si enhanced rhizospheric enzyme activities. Positive correlations were observed between rhizosphere nutrient availability and plant biomass, enzyme activities and Cd accumulation. Transcriptome analysis showed a higher expression of stress responsive transcription factors (WRKY, basic leucine zipper (bZIP), MYB, NAC) and genes encoding functions involved in metal transport (Heavy Metal ATPases (HMA), Copper Transporter (COPT), chelation (Phytochelatins (PCs), Metallothioneins (MTs), Glutathione (GSH)), and cell wall biosynthesis (Cellulose synthases (Ces), Pectin Methylesterases (PME)).

Conclusions

Si enhanced the tolerance and accumulation of Cd in S. alfredii, underscoring its potential in phytoextraction applications.

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.