Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2024-12-10 DOI:10.1007/s11104-024-07133-4

Junlong Xu, Yudie Shao, Xinhao Rao, Jinliang Tang, Christopher Rensing, Hend Alwathnani, Madeha Ayed Alonazi, Jinlin Zhang, Liming Zhang, Shihe Xing, Wenhao Yang

{"title":"Silicon enhanced phytoextraction of Sedum alfredii Hance by improving growth, Cd tolerance, and remolding rhizospheric properties","authors":"Junlong Xu, Yudie Shao, Xinhao Rao, Jinliang Tang, Christopher Rensing, Hend Alwathnani, Madeha Ayed Alonazi, Jinlin Zhang, Liming Zhang, Shihe Xing, Wenhao Yang","doi":"10.1007/s11104-024-07133-4","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aims</h3><p>Silicon (Si) has been shown to alleviate cadmium (Cd) toxicity in plants, but the effect of Si on hyperaccumulators such as <i>S. alfredii</i> has not been fully elucidated. This study evaluated the impact of exogenous Si on growth, Cd accumulation, and rhizosphere properties of <i>S. alfredii</i>.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Si was applied to <i>S. alfredii</i> and various parameters were assessed, including biomass, shoot Cd content, chlorophyll, carotenoid levels, malondialdehyde (MDA) content, antioxidant enzyme activity, cell wall composition, soil nutrient availability, and rhizosphere enzyme activities. A thorough transcriptomic analysis was also conducted.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Si significantly increased shoot biomass (21.47–104.35%) and Cd accumulation (144.60–747.11%) in <i>S. alfredii</i>. Si also enhanced chlorophyll levels, reduced the malondialdehyde (MDA) content, increased antioxidant enzyme activities, and mitigated Cd-induced oxidative stress. Si boosted cell wall components, thereby facilitating Cd transport in roots. In rhizosphere soil, Si improved alkaline hydrolysis of nitrogen (AHN), available phosphorus (AP), available potassium (AK), dissolved organic carbon (DOC), and increased available Cd content. Si enhanced rhizospheric enzyme activities. Positive correlations were observed between rhizosphere nutrient availability and plant biomass, enzyme activities and Cd accumulation. Transcriptome analysis showed a higher expression of stress responsive transcription factors (WRKY, basic leucine zipper (bZIP), MYB, NAC) and genes encoding functions involved in metal transport (Heavy Metal ATPases (HMA), Copper Transporter (COPT), chelation (Phytochelatins (PCs), Metallothioneins (MTs), Glutathione (GSH)), and cell wall biosynthesis (Cellulose synthases (Ces), Pectin Methylesterases (PME)).</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Si enhanced the tolerance and accumulation of Cd in <i>S. alfredii</i>, underscoring its potential in phytoextraction applications.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"37 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-07133-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and aims

Silicon (Si) has been shown to alleviate cadmium (Cd) toxicity in plants, but the effect of Si on hyperaccumulators such as S. alfredii has not been fully elucidated. This study evaluated the impact of exogenous Si on growth, Cd accumulation, and rhizosphere properties of S. alfredii.

Methods

Si was applied to S. alfredii and various parameters were assessed, including biomass, shoot Cd content, chlorophyll, carotenoid levels, malondialdehyde (MDA) content, antioxidant enzyme activity, cell wall composition, soil nutrient availability, and rhizosphere enzyme activities. A thorough transcriptomic analysis was also conducted.

Results

Si significantly increased shoot biomass (21.47–104.35%) and Cd accumulation (144.60–747.11%) in S. alfredii. Si also enhanced chlorophyll levels, reduced the malondialdehyde (MDA) content, increased antioxidant enzyme activities, and mitigated Cd-induced oxidative stress. Si boosted cell wall components, thereby facilitating Cd transport in roots. In rhizosphere soil, Si improved alkaline hydrolysis of nitrogen (AHN), available phosphorus (AP), available potassium (AK), dissolved organic carbon (DOC), and increased available Cd content. Si enhanced rhizospheric enzyme activities. Positive correlations were observed between rhizosphere nutrient availability and plant biomass, enzyme activities and Cd accumulation. Transcriptome analysis showed a higher expression of stress responsive transcription factors (WRKY, basic leucine zipper (bZIP), MYB, NAC) and genes encoding functions involved in metal transport (Heavy Metal ATPases (HMA), Copper Transporter (COPT), chelation (Phytochelatins (PCs), Metallothioneins (MTs), Glutathione (GSH)), and cell wall biosynthesis (Cellulose synthases (Ces), Pectin Methylesterases (PME)).

Conclusions

Si enhanced the tolerance and accumulation of Cd in S. alfredii, underscoring its potential in phytoextraction applications.

查看原文本刊更多论文

硅通过改善生长、镉耐受性和重塑根际特性来促进景天的植物提取

背景与目的硅（Si）已被证明可以减轻植物体内镉（Cd）的毒性，但硅对S. alfredii等超蓄积体的影响尚未完全阐明。本研究评价了外源硅对紫花苜蓿生长、Cd积累和根际特性的影响。方法采用si对紫苏进行生物量、地上部Cd含量、叶绿素、类胡萝卜素、丙二醛（MDA）含量、抗氧化酶活性、细胞壁组成、土壤养分有效性和根际酶活性进行测定。还进行了彻底的转录组学分析。结果si显著提高了紫荆茎部生物量（21.47 ~ 104.35%）和Cd累积量（144.60 ~ 747.11%）。硅还能提高叶绿素水平，降低丙二醛（MDA）含量，增加抗氧化酶活性，减轻cd诱导的氧化应激。硅提高了细胞壁成分，从而促进了镉在根中的运输。在根际土壤中，Si改善了氮（AHN）、速效磷（AP）、速效钾（AK）、溶解有机碳（DOC）的碱性水解，提高了有效镉含量。硅提高了根际酶活性。根际养分有效性与植物生物量、酶活性和Cd积累呈显著正相关。转录组分析显示，胁迫响应转录因子（WRKY、碱性亮氨酸zip （bZIP）、MYB、NAC）和金属转运（重金属atp酶（HMA）、铜转运蛋白（COPT））、螯合（植物螯合蛋白（PCs）、金属硫蛋白（MTs）、谷胱甘肽（GSH））和细胞壁生物合成（纤维素合酶（Ces）、果胶甲基化酯酶（PME））编码功能基因表达较高。结论si增强了紫花苜蓿对Cd的耐受性和积累能力，提示其在植物提取中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.