Assessment of detoxification enzymes in pyrethroid-resistant Listronotus maculicollis Kirby suggests additional mechanisms

IF 2 3区 农林科学 Q2 AGRONOMY
Crop Science Pub Date : 2024-12-09 DOI:10.1002/csc2.21419
Garrett Y. Price, Benjamin A. McGraw
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Abstract

Insecticide resistance is a pressing issue in urban pest management. The annual bluegrass weevil (ABW), Listronotus maculicollis Kirby, is a highly destructive turfgrass pest in northeastern North America. The overuse of pyrethroids has led to resistance in some ABW populations in the northeastern United States. Understanding ABW resistance mechanisms is pivotal for sustaining turf quality and minimizing environmental impact. This study investigated the potential for enzymatic detoxification, a common resistance mechanism in insects, to be the mechanism behind ABW tolerance to pyrethroids. Different ABW populations’ resistance levels to bifenthrin were determined through dose–response Petri dish assays. Cytochrome P450 activity and protein concentrations were measured following bifenthrin exposure. Results indicated golf course-specific effects on enzyme and protein levels but found no direct correlation between these levels and bifenthrin dosage. P450 activity varied among different golf course populations, showing no dependence on bifenthrin dosage. These findings question prior studies suggesting P450 involvement in bifenthrin detoxification in populations of all resistance levels. This study establishes a foundation for comprehending ABW resistance mechanisms and devising effective management strategies. However, further research is needed to investigate additional detoxification enzymes and other resistance mechanisms.

Abstract Image

对拟除虫菊酯抗性黄斑绒螯虾解毒酶的评估提示了其他的机制
杀虫剂抗药性是城市有害生物治理中亟待解决的问题。一年一度的蓝草象鼻虫(ABW)是北美东北部草坪草的一种极具破坏性的害虫。在美国东北部的一些ABW种群中,过度使用拟除虫菊酯导致了抗药性。了解ABW抗性机制对于维持草坪质量和减少对环境的影响至关重要。本研究探讨了酶解毒(昆虫常见的抗性机制)可能成为ABW对拟除虫菊酯耐受的机制。采用剂量反应培养皿法测定不同种群对联苯菊酯的抗性水平。接触联苯菊酯后测定细胞色素P450活性和蛋白浓度。结果表明,高尔夫球场对酶和蛋白质水平有特异性影响,但发现这些水平与联苯菊酯剂量之间没有直接关联。P450活性在不同高尔夫球场人群中存在差异,与联苯菊酯剂量无关。这些发现质疑了先前的研究,这些研究表明P450参与了所有抗性水平种群的联苯菊酯解毒。本研究为理解ABW抗性机制和制定有效的管理策略奠定了基础。然而,需要进一步研究其他解毒酶和其他抗性机制。
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来源期刊
Crop Science
Crop Science 农林科学-农艺学
CiteScore
4.50
自引率
8.70%
发文量
197
审稿时长
3 months
期刊介绍: Articles in Crop Science are of interest to researchers, policy makers, educators, and practitioners. The scope of articles in Crop Science includes crop breeding and genetics; crop physiology and metabolism; crop ecology, production, and management; seed physiology, production, and technology; turfgrass science; forage and grazing land ecology and management; genomics, molecular genetics, and biotechnology; germplasm collections and their use; and biomedical, health beneficial, and nutritionally enhanced plants. Crop Science publishes thematic collections of articles across its scope and includes topical Review and Interpretation, and Perspectives articles.
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