Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks.

IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES
Ticks and Tick-borne Diseases Pub Date : 2024-11-01 Epub Date: 2024-12-08 DOI:10.1016/j.ttbdis.2024.102421
Anna Kloc, Angelina Wójcik-Fatla, Piotr Paprzycki, Lech Panasiuk
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引用次数: 0

Abstract

Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens. In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts. DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp. In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic.

皮acentor reticulatus蜱中立克次体、类弗兰西斯菌内生体和螺原体的跨蜱传播。
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来源期刊
Ticks and Tick-borne Diseases
Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY
CiteScore
6.90
自引率
12.50%
发文量
185
审稿时长
6-12 weeks
期刊介绍: Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.
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