Transovarial transmission of Rickettsia spp., Francisella-like endosymbionts, and Spiroplasma spp. in Dermacentor reticulatus ticks

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES

Ticks and Tick-borne Diseases Pub Date : 2024-11-01 DOI:10.1016/j.ttbdis.2024.102421

Anna Kloc , Angelina Wójcik-Fatla , Piotr Paprzycki , Lech Panasiuk

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引用次数: 0

Abstract

Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp. on the efficiency of the egg-laying process and transmission of selected pathogens.

In total, 16,600 eggs were obtained under laboratory conditions from 55 females, with an average of 346 eggs per female. Adults, eggs, and hatched larvae were tested using polymerase chain reaction (PCR) for the presence of Rickettsia and endosymbionts.

DNA fragments of Rickettsia spp. were found in females (56.4 %) and in pools of eggs (72.9 %) and larvae (62.4 %). FLEs and Spiroplasma endosymbionts were confirmed in females (80 % and 14.5 %, respectively), pools of eggs (81.6 % and 26.1 %, respectively), and larvae (82.7 % and 46.2 %, respectively). Transovarial transmission was confirmed in Rickettsia raoultii, FLEs, and Spiroplasma ixodetis. No correlation was observed between the occurrence of individual endosymbionts and the efficiency of egg laying and transovarial transmission in Rickettsia spp.

In conclusion, transovarial transmission of Rickettsia spp., FLEs and Spiroplasma spp. in D. reticulatus plays an important role in their persistence and circulation in the environment. However, further research is required on this topic.

查看原文本刊更多论文

皮acentor reticulatus蜱中立克次体、类弗兰西斯菌内生体和螺原体的跨蜱传播。

对蜱为宿主和媒介的病原体经卵巢传播的研究，使人们了解了与自然疫源地中选定微生物的循环和持久性有关的机制。本研究的主要目的是探讨立克次体在网纹革蜱中经卵巢传播的可能性，以及弗朗西斯菌样内共生体和螺旋体对产蛋效率和选定病原体传播的影响。在实验室条件下，共从55只雌蚊获得16,600个卵，平均每只雌蚊获得346个卵。采用聚合酶链反应（PCR）检测成虫、卵和孵化的幼虫是否存在立克次体和内共生菌。在雌体（56.4%）、卵池（72.9%）和幼虫池（62.4%）中发现立克次体DNA片段。在雌虫、卵池和幼虫中分别发现内生菌（分别为80%和14.5%）、孢子虫（分别为81.6%和26.1%）和螺体（分别为82.7%和46.2%）。经社会传播在拉乌尔立克次体、血吸虫和杆状螺旋体中得到证实。立克次体的产蛋效率和经卵巢传播与个体内共生菌的发生无相关性。由此可见，立克次体、猪体和螺旋体在网纹田鼠体内的经卵巢传播对其在环境中的持续和循环起着重要作用。然而，这一课题还需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.